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Enzymes . Chemicals . Solutions .
www.enzymicals.com
NEWPROT Kick-off meeting: 25.01.2012
Partner profile Enzymicals AGDr. Rainer Wardenga
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Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes (CEO) Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof. Uwe Bornscheuer (Chairman)Dr. Dagmar BraunDr. Jürgen Eck
Ownership structure
Shareholders and strategic investor
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Northern Germany, Biotechnikum Greifswald
Company Info
www.enzymicals.com
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Company Info
www.enzymicals.com
Northern Germany, Sinetific Campus Greifswald
Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes (CEO) Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof. Uwe Bornscheuer (Chairman)Dr. Dagmar BraunDr. Jürgen Eck
Ownership structure
Shareholders and strategic investor
![Page 4: Enzymes. Chemicals. Solutions. N EW P ROT Kick-off meeting: 25.01.2012 Partner profile Enzymicals AG Dr. Rainer Wardenga](https://reader035.vdocuments.mx/reader035/viewer/2022062516/56649e395503460f94b2aee7/html5/thumbnails/4.jpg)
Management and staff
Dr. U. Menyes (Chemist), CEO
Dr. R. Wardenga (Biologist and Biotechnologist), CSO Dipl. S. Weber (Economist), finance and controlling
Biotechnologists (main focus molecular biology)
Biotechnologist (main focus biocatalysis)
Chemical technical assistant
Biological technical assistant
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Company Info
www.enzymicals.com
Scientific staff
Company management
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Business Segments
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Company Info
www.enzymicals.com
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Institute of Biochemistry at the University of Greifswald (Prof. Bornscheuer)
B.R.A.I.N. AG
HERBRAND PharmaChemicals GmbH
Institute of Chemistry at the University of Rostock (Prof. Kragl)
Institute of Marine Biotechnology e.V. Greifswald
www.enzymicals.com
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Partners
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Research & Development Along the Value Chain
www.enzymicals.com
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Core competence in protein science and development of biocatalytic process
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www.enzymicals.com
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Research & Development Along the Value Chain
Identification of suitable catalysts
Method development and routine application of HPLC, CE, GC and GC-MS
Design and application of spectrophotometric assaysSynthesis of screening substrates
Screening of enzymes or strain collections Search in literature or pathway databasesSearch of known or novel motives in sequence
databases
Amplification of novel genes from genomic DNADe-novo genome sequencing and analysis
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www.enzymicals.com
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Cloning, expression and purification
Fully equipped molecular laboratoriesState of the art expertise in molecular cloningHuge repertoire of expression vectors
Well-founded knowledge of a range of recombinant expression systems
Approved solutions for proteins which are difficult to express
Screening of refolding conditions for aggregated proteins
Production capacity up to 30 L-scaleCatalysts are available in different grades of purity
Purification via affinity-chromatography or fusion-tags
Research & Development Along the Value Chain
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www.enzymicals.com
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Cloning, expression and purification in collaboration with B.R.A.I.N AG
Peptide and Protein Production in
· Escherichia coli / Bacillus sp.
· Pseudomonas stutzeri / Pichia pastoris
· Streptomyces sp.
· Aspergillus sp.
Production Systems:
Optimized Production of Peptides or Proteins in Industrial Scale
Research & Development Along the Value Chain
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www.enzymicals.com
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Biochemical, physiochemical and structural characterization
Determination of catalytic properties and kinetic parameters
Characteristics in temperature- and pH-profilesInvestigation of storage and process stability
Properties via gel-electrophoresis and blottingSecondary structure via CD-spectrophotometry
Screening for crystallization conditions via commercial kits
Access to MALDI-TOF, 2D-gel-electrophoresis
General immobilization studies
Research & Development Along the Value Chain
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www.enzymicals.com
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Protein engineering
Selection of best engineering approaches for designated targets
Approaches often inspired by structural alignments or molecular modeling
Homology-model construction and hot-spot identification Rational design of variants by site-directed mutagenesis
Directed evolution with high quality variant librariesSelection assays and library screening with high-
throughput systems
HT-screening of mutant-librariesAccess to 3-laser flow cytometer with cell sorter
Research & Development Along the Value Chain
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www.enzymicals.com
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Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Catalysis in multi-phase systems or ionic liquids Membrane processes for catalyst recovery and
downstream processing
Research & Development Along the Value Chain
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www.enzymicals.com
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Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Catalysis in multi-phase systems or ionic liquids Membrane processes for catalyst recovery and
downstream processing
Scope of our biocatalytic tollbox
Research & Development Along the Value Chain
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www.enzymicals.com
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NEWPROT: Objectives
(R)-selective amine-transaminases
Catalyze the transfer an amino group from a primary amine to a prochiral ketone or aldehyde
Usable for the resolution of racemic amines or the synthesis of chiral amines
Pyridoxal-5‘-phosphate dependent
Typical amines : alanine, ethylamine, 1- and 2-propylamine, 1- and 2-butylamine ….
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www.enzymicals.com
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(R)-selective amine-transaminases
Catalytic tool box for the evaluation of synthesis routes
Conversion of aliphatic, arylaliphatic and aromatic ketones with strict (R)-selectivity
pH-optima range between 7.0 and 8.5
Alanine serves as amine donor
Products obtained by asymmetric synthesis with ECS-ATA01-07.
NEWPROT: Objectives
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www.enzymicals.com
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Baeyer-Villiger monooxygenases
Flavoenzymes from the class of oxidoreductases
Oxidation of linear, cyclic and aromatic ketones to esters or lactones
Usable with cofactor regeneration or as whole cell system
Known applications:
Biooxidation of functionalized cycloketones
Biooxidation of bicyclic ketones or chiral b-substituted cycloketones
Kinetic resolution of racemic a-substituted cycloketones
Regiodivergent biooxidation of fused bicyclobutanones
NEWPROT: Objectives
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www.enzymicals.com
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Baeyer-Villiger monooxygenases
Catalytic tool box for the evaluation of synthesis routes
Baeyer-Villiger oxidation of aromatic and aliphatic ketones, able to perform sulfur oxidations
High regio- and enantioselectivity, NAD(P)H-dependent
Scope of the ECS biooxidation-toolbox. Examples of products obtained from the corresponding ketones with ECS-M01-07.
O
HN O
O
ee > 99%
OO
OO
MeMe
ee > 99%
ee > 99%
S
O
O
O
Me
ee > 98%
O
H
H
O
ee > 99%
O
ee > 98%
O
Cl
O
HN O
O
ee > 99%
OO
OO
MeMe
ee > 99%
ee > 99%
S
O
O
O
Me
ee > 98%
O
H
H
O
ee > 99%
O
ee > 98%
O
Cl
NEWPROT: Objectives
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www.enzymicals.com
Thank you for your attention
Enzymicals AG,
Walther-Rathenau-Straße 49a17489 GreifswaldFon: +49(0)3834 515-47-0Fax: +49(0)3834 515-47-3