![Page 1: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/1.jpg)
Dopamine in Male Sexual Behavior
Elaine M. Hull
The Florida State University
Psychology Department & Neuroscience Program
![Page 2: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/2.jpg)
Neural circuits regulating sexual behaviorNeural circuits regulating sexual behavior
OB
MeA
MPOA
NAc
BST
VTA
Brain Stem
CTF
![Page 3: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/3.jpg)
Neural circuits regulating sexual behaviorNeural circuits regulating sexual behavior
OB
MeA
MPOA
NAc
BST
VTA
Brain Stem
CTF
![Page 4: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/4.jpg)
Neural circuits regulating sexual behaviorNeural circuits regulating sexual behavior
OB
MeA
MPOA
NAc
BST
VTA
Brain Stem
CTF
![Page 5: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/5.jpg)
Neural circuits regulating sexual behaviorNeural circuits regulating sexual behavior
OB
MeA
MPOA
NAc
BST
VTA
Brain Stem
CTF
![Page 6: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/6.jpg)
Neural circuits regulating sexual behaviorNeural circuits regulating sexual behavior
OB
MeA
MPOA
NAc
BST
VTA
Brain Stem
CTF
![Page 7: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/7.jpg)
Our lab has focused on the medial preoptic area, which contains A14 periventricular DA neurons. It is the main integrative area for male sexual behavior in all vertebrate species.
![Page 8: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/8.jpg)
Model for MPOA dopamine’s influence on male sexual behavior
![Page 9: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/9.jpg)
Is dopamine released in the MPOA during copulation?
![Page 10: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/10.jpg)
Gonadally intact males and T-treated castrates had increased extracellular DA during pre-exposure to a female and during copulation. Vehicle-treated 1-week castrates that copulated also showed DA increases, but those that did not copulate did not show the DA increase.
![Page 11: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/11.jpg)
What elicits the MPOA DA release?
![Page 12: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/12.jpg)
Large lesions of the amygdala abolished copulation, which was restored by
apomorphine in the MPOA.
![Page 13: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/13.jpg)
Smaller lesions of the MeA impaired, but did not abolish mating.
![Page 14: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/14.jpg)
Basal DA levels in the MPOA were normal, but
the DA response to the female was abolished.
![Page 15: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/15.jpg)
Chemical stimulation of the MeA mimicked the MPOA DA response to a female.
![Page 16: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/16.jpg)
![Page 17: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/17.jpg)
Therefore, normal basal DA in the MPOA is sufficient for suboptimal copulation. The DA increase in response to a female facilitates
mating and is mediated by input from the MeA.
• But there are no DA neurons in the MeA. What elicits the DA increase?
![Page 18: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/18.jpg)
Glutamatergic axons from the MeA and BNST to MPOA
• Juan Dominguez, my former post-doc, showed that a few axons from the MeA, and numerous axons from the BNST, ended in the MPOA and contained glutamate.
![Page 19: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/19.jpg)
Is glutamate released in the MPOA before and during mating?
![Page 20: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/20.jpg)
Sample
BL PRE COP EJAC PEI POST
Glu
tam
ate
% c
han
ge
0
100
200
300
400**
*
Sexual activity increases glutamate in the MPOA of male rats.
Using 2 min microdialysis samples
![Page 21: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/21.jpg)
Samples
BL UPTKi PRE COP EJAC PEI POST
Glu
tam
ate
% c
han
ge
0
100
200
300
400ACSFUPTKi
0
100
200
300
400
0
100
200
300
400
500
600
EJACULATIONFREQUENCY
EJACULATIONLATENCY (sec) PEI (sec)
Reverse dialysis of glutamate uptake inhibitors into the MPOA increased glutamate levels and facilitated mating.
**
0
1
2
3
4
5 *** *
![Page 22: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/22.jpg)
Therefore, glutamate is released in the MPOA during copulation, and it facilitates mating.
• Does glutamate also affect MPOA DA levels?
![Page 23: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/23.jpg)
Sample (6 min)
DO
PA
C %
C
HA
NG
EH
VA
%
CH
AN
GE
Sample (6 min)
Exogenous glutamate in the MPOA increased DA levels, but decreased DOPAC and HVA
DA
%
CH
AN
GE
![Page 24: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/24.jpg)
Nitric oxide has been reported to inhibit DA transport and increase DA levels in the striatum.
• Could NO explain our results?
![Page 25: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/25.jpg)
L-NAME blocked the glutamate-evoked DA release and the decreases in
DOPAC and HVA.
DA
%
CH
AN
GE
Sample (6 min)
DO
PA
C %
C
HA
NG
EH
VA
%
CH
AN
GE
Sample (6 min)
Metabolite levels were lower for animals receiving glutamate alone compared with those receiving glutamate+ L-NAME.
![Page 26: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/26.jpg)
Exogenous glutamate in the MPOA increased DA levels, but inhibited
metabolites: Role for NO?POSSIBLE EXPLANATIONS FOR
THIS EFFECT:
1. Glutamate induces exocytocis of DA
2. Glutamate binds NMDA receptors, which allows for Ca2+ influx and induces NO production in NOS- containing cells.
a) Increased NO may inhibit DA uptake in neighboring terminals, prolonging DA’s effects & decreasing DA catabolism.
b) Increased NO might also increase extracellular DA by inducing vesicular leakage.
![Page 27: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/27.jpg)
MPOA dopamine release during copulationdepends on nitric oxide
L-NAME blocked mating-induced DA release in the MPOA.
![Page 28: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/28.jpg)
Microinjection of L-NAME into the MPOA impaired copulation in sexually naïve (A) and experienced (B) males.
A B
![Page 29: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/29.jpg)
Sexually Experienced Males
1. Show increased preference for being with a receptive female.
![Page 30: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/30.jpg)
Sexually Experienced Males
1. Increased preference for being with a receptive female.
2. Require less time and stimulation to achieve ejaculation.
![Page 31: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/31.jpg)
Sexually Experienced Males
1. Increased preference for being with a receptive female.
2. Require less time and stimulation to achieve ejaculation.
3. Require less time to resume copulation after ejaculating.
![Page 32: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/32.jpg)
Sexually Experienced Males
1. Increased preference for being with a receptive female.
2. Require less time and stimulation to achieve ejaculation.
3. Require less time to resume copulation after ejaculating.
4. Are more resistant to sexual impairments due to castration, brain damage, or stress.
![Page 33: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/33.jpg)
Sexually Experienced Males
1. Increased preference for being with a receptive female
2. Require less time and stimulation to achieve ejaculation
3. Require less time to resume copulation after ejaculating
4. Are more resistant to sexual impairments due to castration, brain damage, or stress
Is the MPOA implicated?Is the MPOA implicated?
![Page 34: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/34.jpg)
Stronger Activation of the MPOA in Sexually Experienced Males
Sexually experienced males had more Fos-ir in the MPOA resulting from mating to one ejaculation, than did naïve males that mated for the first time.
First Experience
Repeated Experience
![Page 35: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/35.jpg)
Does NOS in the MPOA contribute to exposure-induced enhancement of mating?
L-NAME administration before each of seven non-copulatory exposures to an estrous female blocked exposure-induced enhancements on the drug-free test day. (Preliminary data suggest that a D1 antagonist has similar effects.)
![Page 36: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/36.jpg)
NNS NS ENS ES
% NOS w/NR1P
erce
ntag
e
0
25
50
75
100
Total NOS
# Cel
ls
NNS NS ENS ES0
20
40
60
80
* **
Sexual experience increases NOS-ir in the MPOA of male
rats
NOS
NMDAR1
Overlay
Nearly all cells containing NOS also contained NMDA receptors. Sexual experience increased the number of NOS-ir cells in MPOA.
![Page 37: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/37.jpg)
Sexually Naïveand Mated
(NM)
SexuallyExperienced
and Not Mated(EC)
Sexually Naïveand Not Mated
(NC)
SexuallyExperiencedand Mated
(EM)M
EA
N D
EN
SIT
Y
(PIX
ELS
)
0
50
100
150
NC NM EC EM
* *
*
Sexual experience increases NOS protein concentration in the MPOA of male rats
![Page 38: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/38.jpg)
A major means of activating NOS is via NMDA glutamate receptors. Does an NMDA antagonist in the MPOA also impair sexual sensitization?
![Page 39: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/39.jpg)
Blocking NMDA receptors in the MPOA impaired sexual sensitization.
Microinjecting MK-801 before each noncopulatory exposures to an estrous female impaired exposure-induced enhancements of:
1. number of mounts
0
2
4
6
8
10
12
14
16
MO
UN
TS
Saline
MK801
Naive*
![Page 40: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/40.jpg)
0
2
4
6
8
10
12
14
16
18
INT
RO
MIS
SIO
NS
Saline
MK801
Naive
Blocking NMDA receptors in the MPOA impaired sexual sensitization.
Microinjecting MK-801 prior to repeated noncopulatory exposures to an estrous female impaired experience-induced enhancements on:
1. number of mounts2. number of intromissions
*
![Page 41: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/41.jpg)
0
0.5
1
1.5
2
2.5
EJA
CU
LA
TIO
NS
Saline
MK801
Naive
Blocking NMDA receptors in the MPOA impaired sexual sensitization.
Microinjecting MK-801 prior to repeated noncopulatory exposures to an estrous female impaired experience-induced enhancements on:
1. number of mounts2. number of intromissions3. number of ejaculations
*
![Page 42: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/42.jpg)
• What intracellular messenger mediates NO’s effects?
![Page 43: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/43.jpg)
Inhibition of guanylyl cyclase blocked effects of the NO donor (sodium nitroprusside).
Cyclic GMP mediates NO’s facilitation of DA release in the MPOA
![Page 44: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/44.jpg)
Cyclic GMP mediates NO’s facilitation of DA release in the MPOA
Inhibition of NOS did not affect facilitation by cGMP analog, because cGMP is downstream of NOS.
![Page 45: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/45.jpg)
Summary
NOS
NM
DA
r
Glutamate
Dopamine
Sexual stimulation
![Page 46: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/46.jpg)
NO
Summary
NOS
NM
DA
r
Glutamate
Dopamine
Sexual stimulation
![Page 47: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/47.jpg)
NO
Summary
NOS
NM
DA
r
Glutamate
Dopamine
Sexual stimulation
(Higher NOSw/ experience)
![Page 48: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/48.jpg)
Postscript: Orexin/hypocretin increases mesolimbic DA activity and facilitates
copulation
![Page 49: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/49.jpg)
Copulation increased Fos-ir in orexin-containing
neurons of perifornical LH.
Double-labeled cells in Non-copulating males
Double-labeled cells in copulating males
![Page 50: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/50.jpg)
Castration decreased
orexin-containing cells; E2
restored them.
![Page 51: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/51.jpg)
Orexin (.014 nmol) in the VTA increased firing rates
of DA neurons.
Orexin (1.4 nmol) in the VTA increased cells per track,
but not firing rates.
Orexin (140 nmol) in the VTA decreased cells per track, probably due to
depolarization block, an effect reversed by
autoreceptor stimulation by
systemic apomorphine.
![Page 52: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/52.jpg)
Red orexin-containing axons end near green TH-containing neurons that are activated (Fos-ir, black) by
copulation.
![Page 53: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/53.jpg)
• Estrogen receptor-containing cells in the MPOA, BNST, and LHA orexin-containing cells in the LHA. Axons from those cells depolarize DA-ergic cells in the VTA and, perhaps, the MPOA. Microinjection of orexin into the MPOA facilitates male sexual behavior (Gulia et al., 2003). 5-HT is released in the LHA at the time of ejaculation and inhibits mating. It also decreases DA release in the NAc (Lorrain et al., 1999) and hyperpolarizes orexin neurons (Li et al., 2002).
![Page 54: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/54.jpg)
Acknowledgments Acknowledgments
K02-MH001714R01-MH040826
Former and Present Lab Members:Bradley Lown, Ph.D Linda Rosselli-Austin, Ph.D Rosemary D’Agostino, Ph.D Deborah Kleese Edelstein, Ph.D Mary Solanto, Ph.D Richard Thomas, Ph.D J. Ken Nishita, Ph.D Daniel Bitran, Ph.D Elizabeth A. Pehek, Ph.D Linda C. Band, Ph.DTerrence Bazzett, Ph.D Vincent P. Markowski, Ph.D Robert C. Eaton, Ph.D Jason Moses, Ph.DLeslie Matuszewich, Ph.D Jianfang Du, Ph.D Susan K. Putnam, Ph.D Jon V. Riolo, Ph.DKatie Grausam
Juan Dominguez, Ph.D
Mario Gil
Gwen Lagoda
Lucy Lumley, Ph.D
Daniel Lorrain, Ph.D
Satoru Sato, Ph.D
Anna Vigdorchik
John Muschamp, Ph.D
Harvey Rattus
![Page 55: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/55.jpg)
![Page 56: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/56.jpg)
![Page 57: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/57.jpg)
SNP and 8-Br-cGMP increase DA levels in the MPOA of DHT-treated castrates.
However, SNP was somewhat more effective.
![Page 58: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/58.jpg)
Only SNP increased DOPAC in DHT-Treated Castrates
![Page 59: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/59.jpg)
Only SNP into the MPOA facilitated sexual behavior in DHT treated castrates
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
90.00
100.00
Control 8-Br-cGMP SNP
Percent that Displayed Mounts
PE
RC
EN
T
*
![Page 60: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/60.jpg)
Only SNP into the MPOA facilitates sexual behavior in DHT treated castrates
Control 8-Br-cGMP SNP
Percent that Displayed Intromissions
PE
RC
EN
T
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
90.00
100.00
*
![Page 61: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/61.jpg)
Only SNP into the MPOA facilitates sexual behavior in DHT treated castrates
Control 8-Br-cGMP SNP
Percent that Displayed Ejaculations
PE
RC
EN
T
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
90.00
100.00
*
![Page 62: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/62.jpg)
PEI (Sec)
100 200 300 400 500 600
Glu
tam
ate
% d
ecre
ase
0
100
200
300
400
500
600
Sample
BL PRE COP EJAC PEI POST
Glu
tam
ate
% c
han
ge
0
100
200
300
400
**
*
Magnitude of decrease in glutamate after ejaculation correlates with post-ejaculatory
interval.
![Page 63: Dopamine in Male Sexual Behavior Elaine M. Hull The Florida State University Psychology Department & Neuroscience Program](https://reader036.vdocuments.mx/reader036/viewer/2022062500/56649e4c5503460f94b42576/html5/thumbnails/63.jpg)
Blocking D1 Receptors Attenuated Activation of MPOA During Initial
Experience
Males receiving a D1 antagonist had less Fos-ir in the MPOA after their first sexual experience, than did males receiving vehicle.
Vehicle
D1 Antagonist