Christoph U. Germeier, Zofia Bulinska-Radomska, Lena Dimberg, Matthias H. Herrmann, Jean Koenig, Gerard Branlard, Danela Murariu, Ivana Polisenska,
Rita Redaelli, Valeria Terzi
Avena Genetic Resources for Quality in Human Consumption
(AVEQ)
Project Partners
Owies, Kraków 2009
AVEQ - Outline
Objectives
Work plan
Fusarium pathogens and mycotoxins in oats
Introduction
A European Project on Nutritional Quality in Avena Genetic Resources
Owies, Kraków 2009
AVEQ - Introduction
Targeted action in accordance with the Council regulation (EC) No 870/2004 establishing a Community programme on the conservation, characterisation, collection and utilisation of genetic resources in agriculture
Starting date: 01. 03. 2007
End date: 28. 02. 2011
Duration: 48 months
Owies, Kraków 2009
AVEQ - Objectives
Evaluation, characterisation and documentation of selected oat genetic resources with regard to:
Contents of protein, fat, minerals, starch, ß- glucan and antioxidants
Resistance to Fusarium infection and mycotoxin contamination
Cold tolerance
Grain weight, hull content and test weight
Owies, Kraków 2009
AVEQ:15 Partners from 9 countries
Co-ordinatorP0: Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Quedlinburg, GermanyProject leader – Dr. Christoph U. Germeier
P1: Plant Breeding and Acclimatization Institute (IHAR), National Plant Genetic Resources Centre Radzików, Poland
P2: Svalöf Weibull AB Cereal Breeding Department, Svalöf, Sweden
P3: Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany
P4: Eurofins Analytik GmbH, Hamburg, Germany
Owies, Kraków 2009
AVEQ - Partners
P 9: Vegetal Genetic Resources Bank of Suceava, Suceava, Romania
P8: CRA-GPG, ex Istituto Sperimentale per la Cerealicoltura, Fiorenzuola d'Arda, Italy
P5: Institut National de la Recherche Agronomique (INRA), UMR Amélioration
et Santé des Plantes, Clermont-Ferrand, France
P6: CRA-MAC, ex Istituto Sperimentale Cerealicoltura, Bergamo, Italy
P7: Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden
Owies, Kraków 2009
AVEQ - Partners
P11: Jõgeva Plant Breeding Institute, Jögeva, Estonia
P10: Institute of Plant Genetic Resources “K. Malkov”, Sadovo, Bulgaria
P12: Plant Breeding and Acclimatization Institute - Laboratory of Nutritional Evaluation of Plant Materials, Radzików , Poland
P14: Agricultural Research Institute Kromeriz, Ltd., Czech Republic
P15: Agrotest Fyto, Ltd., Kromeriz, Czech Republic
Owies, Kraków 2009
AVEQ - Materials
Working collection of 600 accessions of Avena genetic resources of different improvement status :
accessions of wild species
selected land races
modern cultivars
Owies, Kraków 2009
Methods – Workpackages (WP)
WP 1: Management and Coordination- Julius Kuehn Institute, Institute for Breeding
Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0)
WP 2: Project working collection - IHAR, Poland, Zofia Bulinska-Radomska (P1);
P0, P5, P6, P8, P9, P10
Plan: 80% cultivated material - 200 landraces, 200 obsolete cultivars, 100 modern cultivars
20% wild Avena species (100 accessions).
WP 2: Project working collection
Species 2008 2009
A. sativa 287 256
A. byzantina 8 16
A. strigosa 15 31
A. abyssinica 2 3
A. barbata 3 1
A. canariensis - 1
A. damascena 2 -
A. fatua 7 9
A. hirtula 1 3
A. hybrida 1 -
A. sterilis 2 3
A. wiestii 1 -
329 323
Wild species not obtained
A. agadiriana 7
A. atlantica 10
A. insularis 1
A. longiglumis 16
A. macrostachya 2
A. murphyi 16
A. occidentalis 13
A. pilosa 9
A. prostrata 13
A. vaviloviana 12
A. ventricosa 8
11
WP 2: Origin of the Project Working Collection
Legend
1 - 2
3 - 4
5 - 7
8 - 10
11 - 14
15 - 20
21 - 24
25 - 34
35 - 38
39 - 66
Owies, Kraków 2009
Methods - Workpackages
WP 3: Field experiments and sampling seeds for quality analysis
- Vegetal Genetic Resources Bank of Suceava, Romania, Danela Murariu (P9); P1, P2, P5, P8, P10, P11
Field experiments laid out as augmented block designs Plot size from 2.0m2 to 3.0m2
establishing plots, taking observations in the field and on harvested material - descriptors
13
IHAR
SVGB
IPGR
INRA
SW
CRA-ISC
PBI
Descriptors observed in field experiments
- Days to heading- Days to maturity- Plant height- Lodging- Panicle shape- Occurrence of
diseases- Colour of lemma- Hull content- Yield- Thousand kernel
weight- Test weight
Obligatory: Optional:
- Emergence- Number of fertile tillers - Number of seeds per
panicle
Owies, Kraków 2009
Owies, Kraków 2009
Methods – Workpackages
WP 4: Field experiments with artificial Fusarium inoculation
- Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany, Matthias H. Herrmann (P3); P8 (Italy), P9 (Romania), P14 (Czech Republic)
Field trials, inoculation with Fusarium at 3 locations, in Italy
natural infection Observations in the field and on harvested material Samples for mycotoxin analysis
Owies, Kraków 2009
Methods – Workpackages
WP 5: Fusarium and mycotoxin analysis - Agrotest Fyto, Ltd., Kromeriz, Czech Republic,
Ivana Polisenska (P15); P4, P6, P8
analysis of mycotoxin content (DON, T-2, HT-2): ELISA,
LC-MS/MS
determination of Fusarium species: PCR
Owies, Kraków 2009
Fusarium on oats – basic facts
the symptoms of the infection on panicles in the field are only rarely observable (Gilbert and Tekauz, 2000)
newly described species F. langsethiae (Torp and Adler, 2004; Torp and Nirenberg, 2004) is quite often present on oats - no symptoms, T-2 and HT-2 production
Parrika et al. (2006) - in Finland: F. langsethiae, F. poae, F. culmorum,
F. graminearum, F. avenaceum Imathiu et al. (2006) - in GB: mainly F. langsethiae on oats Kiecana and Perkowski; Kiecana and Mielniczuk (2001) – in Poland:
F. avenaceum, F. culmorum, F. poae, F. sporotrichioides and
F. crookwellense
Owies, Kraków 2009
AVEQ = Avena genetic resources for quality in human consumption
Why DON, T-2 and HT-2 in AVEQ?
DON ZEA (ppb) (ppb)
unprocessed cereals other than durum wheat, oats and maize 1250 100
unprocessed durum wheat and oats 1750 100
DON and ZEA have the same producer – F.graminearum and F.culmorum
Owies, Kraków 2009
Why DON, T-2 and HT-2 in AVEQ?
T-2 and HT-2 are mentioned, but without limits - till now
(31) …… the presence of T-2 and HT-2 toxin can be of concern for public health. Therefore, the development of a reliable and sensitive method, collection of more occurrence data and more investigations/research in the factors involved in the presence of T-2 and HT-2 toxin in cereals and cereal products, in particular in oats and oat products, is necessary and of high priority.
Owies, Kraków 2009
Fusarium and mycotoxins in AVEQ
Field trials, inoculation with F.culmorum, F.graminearum, F.sporotrichioides, F.langesethiae and F.avenaceum on 3 locations, in Italy natural infection
Observations in the field and on harvested material
Mycotoxin analysis – analytical problems: (ELISA vs. LC-MS/MS) – comparison study
Harmonisation of laboratories
inoculated non -inoculated
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LC-MS/MS
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Owies, Kraków 2009
ELISA vs. LC-MS/MS, DON
AVEQ standard cultivars
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Owies, Kraków 2009
ELISA vs. LC-MS/MS, T-2
inoculated non -inoculated
AVEQ standard cultivars
Owies, Kraków 2009
Fusarium spp. on oat
cv. Belinda
Owies, Kraków 2009
Analysis for protein (including Avenin patterns), fat and minerals
Analytical WP: 6 - 8
Analysis for starch, fibre and ß- Glucan
Analysis for avenanthramides and tocols
WP 9: Analysis for cold and drought tolerance- Italy, Romania
Owies, Kraków 2009
WP 10: Project documentation and Internet portal
- Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0)
http://eadb.bafz.de/aveq/
All results will be made available online in a crop portal for Avena based on the European Avena Database (EADB).
Owies, Kraków 2009
Thank you for your attention
Acknowledgements
Peter Koelln KGaA, Elmshorn, Germany
Emco Ltd., Praha, Czech Republic
Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP), Bonn, Germany
European Commission, DG AGRI council regulation 870/2004 .