Transcript
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Chapter 10 Gene Isolation and Manipulation
Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.
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Finding specific nucleic acids by using gel electrophoresis and
blotting
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Polymerase chain reaction (PCR)
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Sequencing
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The structure of 2’,3’-dideoxynucleotides
Sanger Sequencing (Dideoxy Sequencing)
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The dideoxy sequencing method
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Reading the DNA sequence from an automatic sequencer
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How to amplify an interesting gene
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Making a cDNA clone
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Introduction of a transgene
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Agrobacterium tumefaciens
causal agent of Crown Gall
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The Ti plasmid vector
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The generation of a transgenic plant
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The generation of a transgenic plant
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Pattern of transmission of T-DNA