Download - CBRN mobile lab in Italy-presentation
CBRN mobile laboratories in Italy
Giorgio MariItalian National Institute for Safety and Health (ISPESL), via Urbana 167, Roma, [email protected] tel. +39 06 9789 3307
Giampaolo Giraudi, Mariarosa Bellino, Michele Pazienza7° NBC Regiment, Italian Army, largo Acquaroni 24, Civitavecchia (RM), Italy
Claudio Garibaldi Department of Fire and Rescue Service and Civil Defense, Fire Department of Rome, NBCR, via Genova 3A, Roma, Italy
Corrado LanciaBiotrace Microsafe, via Aurora 27, Fonte Nuova (RM), Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Unconventional (NBC) terrorism threat in Rome
Symbolic importance of the town
Religious events
Political events
Sportive events
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Sampling and approach to the laboratory.(Standard NBC procedures)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Main data of the mobile laboratories
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ITALIAN ARMY MOBILE LABORATORY
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ITALIAN ARMY MOBILE LABORATORY
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ITALIAN ARMY MOBILE LABORATORY
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Technical area
- Air-conditioner
- Hepa and carbon filters
- Electrical panel
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
The shelter architecture*:
- Technical Area (Pos.1)
- Dressing room (Pos.2)
- Decontamination room (Pos.3)
- Laboratory room (Pos.4)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ROOM PRESSURE CRITERIA
POTENTIALLY CONTAMINATED AREA
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Laboratory in a safe area:- Internal pressure below atmospheric- Dispersion of biological/chemical agent is prevented- Environment is favourite- Fire Brigades (always) and Army (standard situation)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Laboratory in a potentially contaminated area:- Internal pressure above atmospheric- Penetration of biological/chemical agent from outside is prevented- Safety of operators is favourite- Army (emergency situation)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
TECHNICAL SOLUTIONS WHICH ALLOW THE PRESERVATION OF THE SAFETY OF THE LAB OPERATORS AND OF THE EXTERNAL
ENVIRONMENT AT THE SAME TIME
1.The laboratory shelter is constructed with a complete tightness in order to prevent any possible leak.2.There is an air filtration system for both air inlet and air outlet. The filtration includes both particulate (so called HEPA filter, High Efficiency Particulate Airborne) and chemical (activated carbon filter).3.There is no contact between the team responsible for collecting samples (wearing scafander) and the laboratory personnel. The ingress of the samples is done in a safe way through a pass box and the samples are delivered directly inside the isolator4.The internal pressure of the isolator is always below the pressure of the room.
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
PASS BOX FOR THE SAMPLES INGRESS
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
PASS BOX FOR THE SAMPLES INGRESS
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ISOLATOR – GENERAL CONCEPT
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
ISOLATOR WITH “NEGATIVE PRESSURE SYSTEM
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
“NEGATIVE PRESSURE” effectiveness tests
0.5 m/s minimum velocity of air inlet in the glove hole
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
AIR FILTERS
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
AIR FILTERS SAFE REPLACEMENT
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Cleaning Systems
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Cleaning Systems
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
CONTAINERS FOR MATERIALS TRANSFER
Rapid Transport Port (RTP)ISO 11933 - 3
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Rapid Transport Port (RTP)ISO 11933 - 3
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Rapid Transport Port (RTP)ISO 11933 - 3
1. approach1. approach 2. connection
isolator
glove
container
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Rapid Transport Port (RTP)
3. door opening3. door opening 4. sample transfer
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Rapid Transport Port (RTP)exterior surfaces are mainteined
uncontaminated
CLE
AN
CLE
AN
5. door closing5. door closing 6. disconnection
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
tubular film for disposal
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
tubular film for disposal
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
BIOLOGICAL ANALYSIS AN DETECTION
According to the STANAG 4632 ed.1 “Deployable NBC analytical laboratory” (STAndard Nato AGreement), inside the biological lab of the Italian Army, the biological detection is performed with two different techniques:
DNA identification by Real-time PCR (polymerase chain reaction) (virus, bacteria)
Antigen identification by Immunological and elettrochemiluminescent detection (virus, bacteria and toxin)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Real Time -PCR
Real-time PCR (polymerase chain reaction) is a laboratory technique based on the polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted DNA molecule that is typical of the requested agent. Through this method the operator can follow the reaction while the amplification is still in progress using fluorescent probes. The fluorescence is measured in real time by a LED as it is released in response to a specific radiation from the source light generated in the thermocycler.
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
BL2 area - Real Time PCR
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
IMMUNOLOGICAL DETECTION
The method is based on immunological reaction (antigen-antibody) with electrochemiluminescent detection.
The antibody is linked to a chemiluminescent label (marker). If there is the reaction antigen/antibody the complex is excited by an electrode and emits a measurable light.
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
SAMPLING TEAM
MOBILE LABORATORY
WASTES TO BE DISPOSED
- ALIQUOTATION- SAMPLES PREPARATION FOR PCR
IMMUNOLOGICAL DETECTION
AUTOCLAVE
PCR
BL 3 BL 2
MATERIAL/WORK FLOW
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
CHEMICAL ANALYSIS
GC-MS (gas chromatography–mass spectrometry system)
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
RADIOLOGICAL ASSESSMENT
The mobile laboratory (fire brigades) has a high purity Germanium (HPGe) detector cooled in a cryostat with liquid nitrogen, which is usually kept in a front-opening lead-lined container for the storage of Marinelli beakers. The detector is linked to an Ortec DigiDART 16k multichannel analyzer that can interface with a laptop PC. The softwares used are: ScintiVision-32 Gamma-Ray Analysis with NaI and GammaVision-32 Gamma-Ray Analysis with HPGe. The DigiDART can be used, if necessary, directly in the field and independently of the shelter, with battery autonomy of up to 9 hours.For drinking water and general liquid analysis there is an LSC (Liquid Scintillation Counter) Hidex mod Triathler with Beta and Alpha Counter, Gamma Counter and Luminometer functions.
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
CBRN mobile laboratories in Italy
Others lab accessories
Refrigerator
Refrigerated Centrifuge
autoclave
hood
THANK YOU !
[email protected]. +39 06 9789 3307
SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA