CASIROZ Fall MeetingCambridge 2004
Participant group 2
Manuela C. BlumenrötherW. Oßwald
Forest Phytopathology, TU Munich
0
4
8
12
16
20
15-Apr 15-May 14-Jun 14-Jul 13-Aug 12-Sep 12-Oct
month
Glu
cose
[mg
* g D
W-1
]
1xO3 sun 1xO3 sha 2xO3 sun 2xO3 sha
*
***
Seasonal pattern of carbohydrates 2003
0
20
40
60
15-Apr 15-May 14-Jun 14-Jul 13-Aug 12-Sep 12-Oct
month
Fruc
tose
[mg
* g D
W-1
]
1xO3 sun 1xO3 sha 2xO3 sun 2xO3 sha
* *
Higher glucose and fructose concentrationsin 2xO3 sun leaves at most time points
mean values per branch ± SE
Seasonal pattern of carbohydrates 2003
0
20
40
60
80
100
15-Apr 15-May 14-Jun 14-Jul 13-Aug 12-Sep 12-Oct
month
Suc
rose
[mg
* g D
W-1
]
1xO3 sun 1xO3 sha 2xO3 sun 2xO3 sha
**
**
0
2
4
6
8
10
12
15-Apr 15-May 14-Jun 14-Jul 13-Aug 12-Sep 12-Oct
Sta
rch
[mg
gluc
ose
equi
vale
nt *
g D
W-1
]
1xO3 sun 1xO3 sha 2xO3 sun 2xO3 sha
****Lower sucrose and starch concentrations in 2xO3 sun and shade leaves at most time points
mean values per branch ± SE
Diurnal pattern of carbohydrates July 2003: Total soluble sugars & starch
100
110
120
130
140
150
12:00 18:00 00:00 06:00
time points
sol.
suga
rs [m
g*gD
W-1
]
1 x O3 2 x O3
0
2
4
6
8
10
12
14
12:00 18:00 0:00 6:00
time points
sta
rch
[mg
gluc
ose
equi
vale
nt *
g D
W-1
]
1 x O3 2 x O3
***100
110
120
130
140
150
12:00 18:00 00:00 06:00
time points
sol.
suga
rs [m
g*gD
W-1
]
1 x O3 2 x O3
Carbohydrates used for repair in 2 x O3 trees
Significantly reduced starch concentrations in 2 x O3 sun leaves at midday
Specific activity of RubisCO and PEPC [nkat*mg protein-1], June 2003; mean values ± SD
0
2
4
6
8
10
12
14
1 x O3 2 x O3 1 x O3 2 x O3
sun shade
treatment & exposition
[nka
t*m
g pr
otei
n-1
]
PEPc RubisCO
no clearly visible effect of ozone on enzyme activity of RubisCO and PEPC
0
4
8
12
16
20
410 412 443 480 410 412 443 480
shade sun
tree no. & exposition
star
ch [m
g gl
ucos
e eq
uiva
lent
*g D
W-1
]
Aug 03
0
4
8
12
16
20
410 412 443 480 410 412 443 480
shade sun
tree No. & exposition
star
ch [m
g gl
ucos
e eq
uiva
lent
*g D
W-1
]
May 03 Aug 03 May 04
Amax360 on selected trees in August 2003
-2
0
2
4
6
8
10
12
Am
ax36
0 [µ
mol
m-2
s-1]
Aug 03
Amax360 on selected trees in August 2003 & May 2004
-2
0
2
4
6
8
10
12
Am
ax36
0 [µ
mol
m-2
s-1]
Aug 03May 04
Parallel assessment ofphotosynthesis andcarbohydrate content(August 03 & May 04)
Pho
tosy
nthe
sis
Sta
rch
2 x O32 x O3 1 x O3
1 x O3
2 x O32 x O31 x O3 1 x O3
Carbohydrates in relation to CO2 uptake (parallel assessment August 2003)
y = -0.7078x + 11.072R2 = 0.3936
0
2
4
6
8
10
12
14
16
-2 0 2 4 6 8
Amax360 [µmol m -2s-1]
Glu
cose
[mg*
g D
W-1
]
Glucose
y = -0.7078x + 11.072R2 = 0.3936
0
2
4
6
8
10
12
14
16
-2 0 2 4 6 8
Amax360 [µmol m -2s-1]
Glu
cose
[mg*
g D
W-1
]
Glucose
y = -3.5935x + 42.861R2 = 0.6733
0
10
20
30
40
50
60
-2 0 2 4 6 8
Amax360 [µmol m -2s-1]
Fruc
tose
[mg*
g D
W-1
]
Fructose
Carbohydrates in relation to CO2 uptake (parallel assessment August 2003)
y = 4.0683x + 61.991R2 = 0.2678
0
10
20
30
40
50
60
70
80
90
100
-2 0 2 4 6 8
Amax360 [µmol m -2s-1]
Suc
rose
[mg*
g D
W-1
]
Sucrosey = 0.1593x + 0.7601
R2 = 0.2562
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
-2 0 2 4 6 8
Amax360 [µmol m -2s -1]
Sta
rch
[mg
gluc
ose
equi
vale
nt*g
DW
-1]
Starch
Carbohydrates in relation to CO2 uptake (August 2003)y = -0.7078x + 11.072
R2 = 0.3936
0
2
4
6
8
10
12
14
16
-2 0 2 4 6 8
y = -3.5935x + 42.861R2 = 0.6733
0
10
20
30
40
50
60
-2 0 2 4 6 8
y = 4.0683x + 61.991R2 = 0.2678
0
10
20
30
40
50
60
70
80
90
100
-2 0 2 4 6 8
y = 0.1593x + 0.7601R2 = 0.2562
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
-2 0 2 4 6 8
Suga
r co n
c en t
rati o
n [m
g *g
DW
-1]
A max [µmol m-2 s-1]
Glucose
Starch
Fructose
Sucrose
Carbohydrates in relation to CO2 uptake (August 2003)
0
2
4
6
8
10
12
14
16
-2 -1 0 1 2 3 4 5 6 7
Amax360 [µmol m-2s-1]
Glu
cose
[mg*
g D
W-1
]
1xO3 sha Aug 032xO3 sha Aug 031xO3 sun Aug032xO3 sun Aug03
0
10
20
30
40
50
60
-2 -1 0 1 2 3 4 5 6 7
Amax360 [µmol m-2s-1]
Fruc
tose
[mg*
g D
W-1
]
1xO3 sha Aug 03 2xO3 sha Aug 03 1xO3 sun Aug03 2xO3 sun Aug03
Carbohydrates in relation to CO2 uptake (August 2003)
0
10
2030
40
50
60
70
80
90
100
-2 -1 0 1 2 3 4 5 6 7
Amax360 [µmol m-2s-1]
Suc
rose
[mg*
g D
W-1
]
1xO3 sha Aug 032xO3 sha Aug 031xO3 sun Aug032xO3 sun Aug03
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
-2 -1 0 1 2 3 4 5 6 7
Amax360 [µmol m-2s-1]
Sta
rch
[mg
gluc
ose
equi
vale
nt
* g D
W-1
]
1xO3 sha Aug 03 2xO3 sha Aug 03 1xO3 sun Aug03 2xO3 sun Aug03
Sucrose in relation to CO2 uptake (August 03 vs. May 04)
CO2 uptake [µmol m-2 s-1]
Sucr
ose
[mg*
g D
W-1]
Sucrose in relation to CO2 uptake (August 03 vs. May 04)Su
c ros
e [m
g*g
DW
-1]
CO2 uptake [µmol m-2 s-1]
August 03
1 x O3
2 x O3
Sucrose in relation to CO2 uptake (August 03 vs. May 04)Su
c ros
e [m
g*g
DW
-1]
CO2 uptake [µmol m-2 s-1]
May 04
1 x O3
2 x O3
Sucrose in relation to CO2 uptake (August 03 vs. May 04)
CO2 uptake [µmol m-2 s-1]
Sucr
ose
[mg*
g D
W-1]
August 03 May 04
shade
sun
Sucrose in relation to CO2 uptake (August 03 vs. May 04)
CO2 uptake [µmol m-2 s-1]
Sucr
ose
[mg*
g D
W-1]
y = 4.0683x + 61.991R2 = 0.2678
y = 4.0931x + 25.041R2 = 0.2271
0
20
40
60
80
100
-2 0 2 4 6 8 10 12 14
Aug-03 May-04
Starch pattern in sun leaves; October 2003
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
399 408 409 410 412 437 439 443 480 482
tree No.
star
ch [m
g gl
ucos
e eq
uiva
lent
*g d
w-1
]
07.10.2003 16.10.2003
1 x O3 2 x O3
Thank you!!!
Seasonal pattern of carbohydrates in young trees; MV per treatment ± SE
0
20
40
60
80
100
Jun-03 Jul-03 Aug-03 Sep-03
month
sucr
ose
[mg*
gDW
-1]
0
4
8
12
16
20
star
ch [m
g gl
ucos
e eq
uiva
lent
*g D
W-1
]
suc 1xO3 sun suc 2xO3 sun suc 1xO3 sha suc 2xO3 shasta 1xO3 sun sta 2xO3 sun sta 1xO3 sha sta 2xO3 sha
0
10
20
30
40
50
Jun-03 Jul-03 Aug-03 Sep-03
month
gluc
ose
[mg*
g D
W-1
]
0
10
20
30
40
50
fruc
tose
[mg*
g D
W-1
]
glu 1xO3 sun glu 2xO3 sun glu 1xO3 sha glu 2xO3 shafru 1xO3 sun fru 2xO3 sun fru 2xO3 sha fru 1xO3 sha