and
Edited by
Biotechnology and Sustainable
Agriculture 2006 and Beyond
August 31 18, 2006 Beijing, China
Zhihong XuPeking University, China
Jiayang Li
Chinese Academy of Sciences, Beijing, China
Yongbiao Xue
Chinese Academy of Sciences, Beijing, China
Chinese Academy of Sciences, Beijing, China
Weicai Yang
Institute of Genetics and Developmental Biology,
Institute of Genetics and Developmental Biology,
Institute of Genetics and Developmental Biology,
-Proceedings of the 11th IAPTC&B Congress,
A C.I.P. Catalogue record for this book is available from the Library of Congress.
Published by Springer,
www.springer.com
Printed on acid-free paper
All Rights Reserved
No part of this work may be reproduced, stored in a retrieval system, or transmitted
and executed on a computer system, for exclusive use by the purchaser of the work.
P.O. Box 17, 3300 AA Dordrecht, The Netherlands.
© 2007 Springer
exception of any material supplied specifically for the purpose of being entered
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording or otherwise, without written permission from the Publisher, with the
ISBN 978-1-4020-6634-4 (HB)ISBN 978-1-4020-6635-1 (e-book)
v
CONTENTS
PLENARY LECTURES
Zhihong Xu Facing the Future with Pharmaceuticals from Plants…………………….13 Rainer Fischer, Richard M. Twyman, Stephan Hellwig,
Embryogenesis In Vitro…………………………………………………..35 Jim M. Dunwell
Multigene Strategies for Engineering the Phytoremediation of Mercury and Arsenic…………………………………………………..49 Richard B. Meagher, Aaron P. Smith, Melissa Pischke, Tehryung Kim, Om Prakash Dhankher, and Andrew C.P. Heaton
Molecular Control of S-RNase-based Self-Incompatibility……………...63 Jian Huang, Lan Zhao, and Yongbiao Xue The Use of Sexual Model Systems to Identify Elements of Apomixis………………………………………………………………77 Ueli Grossniklaus, Philippa Barrell, Amal Johnston, Ramaurthy Baskar, Jacqueline Gheyselinck, Lukas Brand, and Mark Curtis Agricultural Biotechnology in China: Past, Present and Future………………………………………………………………...81 Valerie J. Karplus and Xing Wang Deng
Plant Biotechnology and Crop Improvement in China……………………3
2006 Science and Technology Exhibitors…............………………….…xix
Opening Speech by Zhihong Xu, President of the IAPTC&B..………....xxi
Jürgen Drossard, and Stefan Schillberg
vi Contents
SYMPOSIA
Current Advances in Plant Tissue Culture Techniques
Regulation of Plant Morphogenesis In Vitro: Role of Ethylene and Polyamines………………………………..……….89 Eng-Chong Pua
Lingfasu and Its Use on Plant Tissue Culture In Vitro……………..…....97
for Plum Pox Potyvirus Resistance……………………………..……….103
Antonet Svircev, Daniel C. Brown, and Rui Wen
An Efficient Novel Method of Producing Virus Free
Site-Directed Homologous Recombination in Tobacco
Charles Q. Cai, W. Michael Ainley, Trevor N. Collingwood, Robbi J. Garrison, Lisa L. Schulenberg, Andrew Worden, Philip D. Gregory, Beth C. Rubin-Wilson, and Joseph F. Petolino
Generation of Genetically Modified Strawberries in Temporary Immersion Bioreactor System…………………….……..115 Kati Hanhineva, Harri Kokko, and Sirpa Karenlampi
Current Advances in Plant Molecular Biology
The Positioning of the Division Plane Is Determined by a Cortical Division Site at the Plasma Membrane…………………...121 Danny Geelen
Repression of Plant Gene Expression via Chromosomal Remodelling Using Histone Deacetylases………………………………125 Lining Tian, Keqiang Wu, Brian Miki, and Dan Brown
Xu Hong-Yuan, Xu Hong-Zhang, He Bing, and Zhou Feng-Jue
Cell Cultures via Zinc Finger Nucleases………………………..………111
Plants from Garlic Root Meristem…………………..………………….107 Md. Shahidul Haque, K. Hattori, A. Suzuki, and T. Tsuneyoshi
Lining Tian, Shuocheng Zhang, HJIPne SanfaHon,
PPV-Specific Hairpin RNAs is an Effective Method
Contents vii
Functional Genomics
Ming Luo, Mingzhu Luo, Fred Berger, E.S. Dennis, Jim W. Peacock, and Abed Chaudhury
Integrating Transcriptional and Metabolic Profiling to Unravel
Heiko Rischer, Matej Oresic, Alain Goossens, Dirk Inzé, and Kirsi-Marja Oksman-Caldentey
Plant Transformation Technology
Fang-Ming Lai, Kangfeng Mei, Luke Mankin, and Todd Jones
Goetz Hensel, Vladimir Valkov, Conny Marthe, and Jochen Kumlehn
in Molecular Biology
Use of Alfalfa In Vitro Cultures in Studies on Regulation of Cyclin-Dependent Kinase (CDK) Functions……………………..…..149 Dénes Dudits, Mátyás Cserháti, Pál Miskolczi, Attila Fehér, Ferhan Ayaydin, and Gábor V. Horváth
Phytohormone Regulation of Cotton Fiber Development In Vitro……...153 Barbara A. Triplett, Hee Jin Kim, Doug Hinchliffe, Sing-Hoi Sze, Peggy Thaxton, David Stelly, and Z. Jeffrey Chen
Patterns of Growth and Cell Death in Diploid Arabidopsis Cell Cultures with Synchronised Cell Cycle……………………............157 Ranjith Pathirana, Phillip West, and Jocelyn Eason
Efficient Agrobacterium-Mediated Transformation of Various Barley (Hordeum vulgare L.) Genotypes……………..……..143
Application of Two New Selectable Marker Genes,
DNA-METHYLTRANSFERASE 1 is a Member of FIS Polycomb
In vitro System as the Model for Discovery
Complex and is Involved in Seed Development in Arabidopsis...............131
Secondary Metabolite Biosynthesis in Plants………………………...…135
dsdA and dao1 in Maize Transformation……………………………..….141
viii Contents
Molecular Control of Abiotic Resistance
Engineering of Reactive Species Detoxification Pathways for Increasing Stress Tolerance in Plants…………………………..……163 Gábor V. Horváth, Zoltán Turóczy, Mátyás Cserháti, Petra Kis, Katalin Török, László Sass, and Dénes Dudits
Orchid Flower Fragrance Biosynthesis Pathway and Its Key Enzyme Geranyl Diphosphate Synthase…………………………..…….167 Yu-Yun Hsiao, Wen-Chieh Tsai, Chang-Sheng Kuoh, Tian-Hsiang Huang, Hei-Chia Wang, Yann-Lii Leu, Tian-Shung Wu, Wen-Huei Chen, and Hong-Hwa Chen
Cytokinin Function in Drought Stress Response and Subsequent Recovery……………………………………..………...171 M. Novakova, P. Dobrev, V. Motyka, A. Gaudinova, J. Malbeck, J. Pospisilova, D. Haisel, H. Storchova,
Xenogenomics: Bioprospecting for Genetic Determinants of Cold and Freezing Stress Tolerance in the Cryophilic Antarctic Hair Grass Deschampsia antarctica E. Desv. …………..……175
D. Gunawardana, and G.C. Spangenberg
Plant Insect Interaction
P.E. Urwin
Molecular Basis of Colorado Potato Beetle Adaptation
N. Slapar, M. Pompe-Novak, M. Buh, M. Ravnikar, B. Štrukelj, M.A. Jongsma, and K. Gruden
Molecular Mechanisms on Plant-Microbe Interactions
An Arabidopsis Nudix Hydrolase Modulates the Innate Immune Response Independent of NPR1 and Salicylate………….……199 Yiji Xia, Xiaochun Ge, Guo-Jing Li, and Huifen Zhu
J. Dobra, M.C. Mok, D.W.S. Mok, R. Martin, and R. Vankova
M.J. Kuiper, J.P. Talbot, O. Chew, E. Ribarev, D. Azria,
Biotechnological Control of Plant Parasitic Nematodes………...............181
to Potato Defence Mechanisms………………………………..........…...191
U.P. John, R.M. Polotnianka, A. Sivakumaran, L. Mackin,
Contents ix
RNA-Mediated Resistance to Cassava Geminiviruses in Transgenic Cassava…………………………………………...………201 Hervé Vanderschuren, Rashid Akbergenov, Mikhail M. Pooggin, Thomas Hohn, Wilhelm Gruissem, and Peng Zhang
The Molecular Basis of Cold-Induced Pollen Sterility in Rice………....205 Sandra N. Oliver, Xiaochun Zhao, Elizabeth S. Dennis, and Rudy Dolferus
Regulation of the Rice NADPH Oxidase by Binding of Small GTPase Rac and Ca2+ to Its N-terminal Extension……………………...209 Hann Ling Wong, Reinhard Pinontoan, Kana Hasegawa, Takashi Yaeno, Koh Iba, Ryo Tabata, Kokoro Hayashi,
Chojiro Kojima, Tsutomu Kawasaki, and Ko Shimamoto
Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza Virus Confers Resistance to the Virus in Transgenic Mexican Lime…………………………..…...211 Carmen Fagoaga, Carmelo López, Alfonso Hermoso de Mendoza, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña
Phloem Feeding Regulates the Plant Defense Pathways Responding to Both Aphid Infestation and Pathogen Infection………...215 Yinghua Huang
Metabolic Engineering
Towards Metabolic Engineering of Carotenoid Content in Sweet-orange (Citrus sinensis (L.) Osb.)………………………..…...223 Marcio G.C. Costa, Amanda F.S. Mendes, Luciana C. Cidade, Walter S. Soares-Filho, Wagner C. Otoni, and Gloria A. Moore
Engineering New Crops for Safe Castor Oil Production………..............227 Grace Q. Chen, Yeh-Jin Ahn, and Louisa Vang
Biochemical and Structural Bases for (Iso)flavonoid Biosynthetic Diversity………………………………………..…………231 Chang-Jun Liu, Wang Cheng, Joseph P. Noel, and Richard A. Dixon
x Contents
Utilization of Plant Metabolic Engineering for Production of Pharmaceuticals in Tea…………………………………………….....235 A. Borthakur, R.K. Dutta, S. Borchetia, and Sudripta Das
Improving Nutritional Quality of Crops
Success Towards Alleviating Peanut Allergy: The Major Allergen Ara h 2 is Silenced via RNA Interference (RNAi)………….…261 K.N. Konan, O.M. Viquez, F.C. Chen, and H.W. Dodo
Transgenic Poplar for Phytoremediation………………..………………265 Won-Yong Song, Young-Im Choi, Donghwan Shim, Do-Young Kim, Eun-Woon Noh, Enrico Martinoia, and Youngsook Lee
The Africa Biofortified Sorghum Project – Applying Biotechnology to Develop Nutritionally Improved Sorghum for Africa……………………………………………………..…………273 Zuo-yu Zhao
Genetic Engineering of Strawberry for Taste Improvement and Enhanced Disease Resistance by Introduction of thauII Gene………….279
Genetic Engineering of Crops for Improved Nutritional Quality……….283 Samuel S.M. Sun, Qiaoquan Liu, and Rebecca M.L. Chan
Transcriptome Profiling of Wheat Genotypes Differing in Bread Baking Quality under Different Environments……………..…289 Yongfang Wan, Rowan Mitchell, Tim Wheeler, Mike Gooding,
Salvador Gezan, Sue Welham, Christina Shenton, Tong Zhu, and Peter Shewry
Signal Transduction
Cotton 14-3-3L Gene Is Preferentially Expressed in Fiber……….….…295 Xue-Bao Li, Hai-Yan Shi, Xiu-Lan Wang, Wen-Liang Xu, Deng-Di Li, and Hong Wang
Rebecca Poole, Keith Edwards, Clare Mills, Peter Skeggs,
K.A. Schestibratov, and S.V. Dolgov
Contents xi
Inositol Polyphosphate 6-/3-kinase (AtIpk2β), an Early Auxin-Responsive Gene, Positively Regulates Axillary Shoot Branching in Arabidopsis thaliana...........................................................299
and Hui-Jun Xia
Apomixis
Genetic Analyses of Aposporous Embryo Sac Formation in Sorghum………………………………………………………………305 John G. Carman, Michelle S. Jamison, Jayasree Pattanayak, Jeff Lacey, Jeong-Soon Kim, Estella G. Elliott, Patricia Klein, Tom Ulrich, and Krishna Dwivedi
Epigenetic Regulation of Seed Development………………..……….…309 Claudia Köhler
Biotechnology in Cereals
Optimisation of Tissue Culture, Regeneration and Agrobacterium-Mediated Transformation Paramaters in Winter Wheat Cultivars (T.durum cv. K z ltan-91
M. Kavas; H.A. Öktem, and M. Yücel
Enhancement of the Brassinosteroid Biosynthesis Pathway
Chuan-Yin Wu, Shing Kwok, Sam Harris, Anthony Trieu, Parthiban Radhakrishnan, Andres Salazar, Ke Zhang, Jiulin Wang, Jianmin Wan, Shozo Fujioka, Ken Feldmann, and Roger Pennell
Yasunari Ogihara
Zai-Bao Zhang, Guang Yang, Zhen Chen, Yan Li, Fernando Arana,
ı ı
Biotechnology in Economic Crops
Isolation and Characterization of Senescence-Associated
and Helen Nair Zuliana Razali, S. Chandran, Lee Ai Ling, A.N. Boyce,
and T.aestivum cv. Bezostaja-01).............................................................315
Improves Grain Yield in Rice………………………………………..….319
Functional Genomics of Hexaploid Wheat…………………….………..323
Ethylene Genes from Dendrobium Orchids………………………..……327
xii Contents
Zeng-Yu Wang, Jeremey Bell, Xiaofei Cheng, Yaxin Ge, Kun Jun Han, Xuefeng Ma, Elane Wright, Yajun Xi, Xirong Xiao, Ji-Yi Zhang, Andrew Hopkins, and Joseph Bouton
Biotechnology in Medicinal, Woody and Horticultural Plants
Toshiaki Umezawa
Polyketide Metabolism in Hypericum perforatum
Ludger Beerhues and Benye Liu
In Vitro Selection and Molecular Markers for Early
F. Eudes, Ana Badea, A. Laroche, D. Gaudet, R. Graf, and S. Sadasivaiah
The Geo-Model for Cell Wall Formation: A Design Tool
Carolina Cifuentes and Anne Mie C. Emons
Tissue Culture for Biodiversity Preservation
Integrating In Vitro Methods for Propagating and Preserving
V.C. Pence, S.M. Charls, B.L. Plair, M.A. Jaskowiak, G.D. Winget, and L.L. Cleveland
Micropropagation and Germplasm Conservation of Staple
Virendra Mohan Verma
Efficient In Vitro Bulblet Regeneration from Immature Embryos of Endemic and Endangered Geophyte Species
Sebahattin Özcan, İskender Parmaksız, Semra Mirici,
and Neşet Arslan a
Satı Çöçü, Serkan Uranbey, Arif İpek, Cengiz Sancak, Erc ment O. S rıhan, Bilal Gürbüz, Cafer S. Sevimay, ü
Biotechnological Improvement of Forage Crops…………………..……333
Lignan and Norlignan Biosynthesis and Biotechnology………...………341
and Related Species…………………………………………..……...….345
Screening of Fusarium Head Blight Resistance Wheat………..………...349
for Plants……………………………………………………………...…353
Endangered Plants……………………………………………………....363
Food Crops and Traditional Medicinal Plants in Micronesia………..….375
in Sternbergia, Muscari and Fritillaria Genera……………………...…381
Contents xiii
Pramod Tandon
Plants as Bioreacter/Molecular Pharming
Elisabetta Lupotto and Maria Rosaria Stile
Somika Bhatnagar, Yuan Qing Deborah Hong,
Production of Recombinant Antibodies in Pea Seeds
Isolde Saalbach, Marcus Riehl, Martin Giersberg, Jochen Kumlehn, and Dieter Falkenburg
Molecular Breeding
Farhad Nazarian Firouzabadi, Geraldine Kok-Jacon,
Kirsten Jørgensen, Charlotte Sørensen, Susanne Jensen, Marc Morant, Martin Fregene, and Birger Lindberg Møller
Evaluation of GM Poplars Expressing Relevant Traits for Herbicide Tolerance, Disease Resistance and Production of Pharmaceutics: Biochemical, Molecular and Microbiological Studies on Plants
F. Mattivi, M. Bonadei, M. Gennaro, E. Quattrini, P. Calligari, F. Picco, G. Deandrea, and A. Balestrazzi
Ji Qin, Jean-Paul Vincken, Luc Suurs, and Richard G.F. Visser
Guang Yuan Wang, and Yan Hong
S. Zelasco, D. Carbonera, A. Giorcelli, M. Confalonieri,
Molecular Breeding of Low-Phytic-Acid Grains in Rice
Mio Kuwano, Makoto Suzuki, Fumio Takaiwa, and Kaoru T. Yoshida
In Vitro Strategies for Conservation of Plant Diversity in India……..…385
Molecular Farming in Plants: An Update……………………..………....389
In Vivo and In Vitro Studies on Huperzine A Producing Ferns…………397
and Their Oral Application in Piglets………………………..……….…399
Breeding for Improved and Novel Starch Characteristics in Potato...…..405
Biofortification of Cassava Using Molecular Breeding…………..….….409
and Detection of Transgene Sequences in Soil………………...........…. 413
by Using the Promoter of 18 kDa Oleosin………………………...…….419
xiv Contents
Regeneration, Micropropagation and Industrialization
A Highly Efficient Protocol for Micropropagation
Sijun Zhou and Daniel C.W. Brown
The Use of Tissue Culture for Successful
Satjaporn Chantawong, Upsorn Pliansinchai, Chalermpol Kirdmanee, and Pipat Weerathaworn
Development of Subclonal Variants from Interspecific
S. Rajeswari, Shini Sekar, and M. Krishnamurthi
Forcing and In Vitro Establishment of Softwood Shoots
Faheem Aftab and John E. Preece
Protoplasts, Haploids and Embryo Culture in Crop Improvement
Introgression Breeding Program in Lolium/Festuca
Toshihiko Yamada, Yang-dong Guo, Yuuko Mizukami, Ken-ichi Tamura, and Kazuhiro Tase
Recent Advances in Anther Culture of Coconut
P.I.P. Perera, V. Hocher, J.L. Verdeil, D.M.D. Yakandawala, and L.K. Weerakoon
Protoplast Isolation and Cultivation from Embryogenic
Jiang Lu, Xia Xu, and Judy Grosser
W.W. Guo, X.D. Cai, Y.J. Cheng, J.W. Grosser, and X.X. Deng
of North American Ginseng……………………………………………..425
and Sustainable Cane………………………………………..……..……429
Hybrids of Sugarcane……………………………………………..…….433
from Large Stem Segments of Woody Plants……………………..…....437
Complex Using Androgenesis…………………………………..………447
(Cocos nucifera L.)…………………………………………..…….……451
Suspension Cultures and Leaves of Grapevines (Vitis L.)……………….457
Protoplast Technology and Citrus Improvement…………..…………....461
Contents xv
A.M.R. Ferrie and T.D. Bethune
Integration of Cold Signal Transduction Pathway Related
Xin Zhou, Fei He, Fengxia Liu, Xue Zheng, Chao Di, Shaoxia Zhou, Hongya Gu, Wenying Xu, and Zhen Su
Rice Genetics and Genomics Information as the Ultimate
Takuji Sasaki, Jianzhong Wu, and Takashi Matsumoto
The Development of Agricultural Biotechnology Capacities in Palestine through the UNESCO Biotechnology Educational
Naim Iraki, Omar Dar-Issa, Basma Sandouka, Michael Sansour, Rami Arafeh, Amani Abu-Sa’da, and Nida’ Salah
Technology Transfer to Developing Countries
Microspore Embryogenesis in the Apiaceae………………..…………...465
to ABA 8’-hydroxylase in Arabidopsis…………………….………..…..471
Tool for Rice Improvement………………………………………...……475
and Training Center at Bethlehem University………………….……….487
Author Index………………………………………………..………….491
xvii
Komamine, Yongbiao Xue (Chair-Local Organizing Commitee, Co-Editorof In Vitro Plant), Eng-Chong Pua (Editor of In Vitro Plant).
From left: Arie Altman, Zhihong Xu (President), Indra K. Vasil, Atsushi
xviii
IAPTC&B President Zhihong Xu announcing the election of Dr. Roger Beachy of USA as President of IAPB for 2006–2010, and of St. Louis as the host city of the 12th Congress in 2010.
Indra K. Vasil on behalf of President Roger Beachy of 12th IAPB (2006–2010) receive the logo from President Zhihong Xu of 2002–2006 of IAPTC&B.
Alto Holdings Limited, New Zealand DBN Group, China Duchefa Biochemie bv, The Netherlands Eppendorf China Ltd., China Phyto Technology Laborateries, USA Sigma-Aldrich Trading Co. Ltd., China Springer, Germany Sunshine Horticulture Co., Ltd., China Yantze Deltaregion Institute of Tsinghua University, Zhejiang, China
2006 Science and Technology Exhibitors
xix
Opening Speech by Zhihong Xu,
President of IAPTC&B 2006
Good Morning, Ladies and Gentlemen!
It has been 4 years since we met in Orlando, USA in June, 2002.
On behalf of the International Association for Plant Tissue Culture and Biotechnology, I would like to express my warm welcome to all the delegates attending the 11th International Congress of Plant Tissue Culture and Biotechnology.
This Congress is certainly a world-class event with over 800 parti-cipants from 94 countries. In the following 5 days, the best and the brightest of international scientific leaders will present 14 plenary, 34 keynote and 120 symposia lectures covering the entire research field of plant biotechnology, as well as laws and policies regarding trans-genic plants and technology transfer. More than 600 posters will augment the formal lecture sessions. The IAPTC&B awarded 15 fellowships to graduate students and post-doctoral associates from developing countries.
The International Association for Plant Tissue Culture and Bio-technology is one of major international professional associations in the field of life science. The mission of the Association is to promote research in all aspects of basic and applied research in plant tissue culture and biotechnology. The theme of this Congress is ‘Biotech-nology and Sustainable Agriculture’. It is a theme that is especially timely for plant biologists working today. I hope that this Congress will further promote global research activities in the field of plant biotechnology and thus make greater contributions to the world people.
Besides scientific activities in the Congress, I hope you will also enjoy your stay in Beijing. Beijing is one of the most attractive and cosmopolitan cities in the world. It is the political and cultural center of ever-growing China, home of 11 million citizens, seat of the Chinese government, a city highly celebrated for its beautiful surroun-dings and well-preserved historic sites and cultural relics. I would
xxi