Download - Biological Applications of Crystallography
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Biological Applications of Crystallography
Joseph D. Ng
University of Alabama in HuntsvilleMay 24, 2014
www.uah.edu/nglab
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Crystallography is NOT……
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34Å
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y
x
h
sin = y/h
cos = x/h
tan = y/x
Bragg’s Law
All you need to know…for now
tan-1(y/x)
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72o
2.8mm
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y
x
h
tan-1(y/x) = 3.2
X-ray
Film
x
x=50mm
y=2.8mm
Bragg’s Law
d= 27.5Å
Let’s calculate Pitch
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cos = x/h cos = d/P P=d/cos
P= 34Å
72o
2.8mm
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y=Rsiny=Rsin(x/P)
dy/dx=Rcos(x/P)/P
So when x=0
dy/dx = R/P
dy/dx =Rcos()/P
X=0Tan (90o-)= R/P
Let’s calculate Radius!
X=0
R = 10Å
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34Å
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Hydrogen-bonding patters in the base pairs defined by Watson and Crick
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Sample of Crystals Obtained
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Advanced Photon Source Argonne National Lab
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2.0 Å
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