BIO341 Meiotic mapping of whole genomes (methods for
simultaneously evaluating linkage relationships among
large numbers of loci)
From: Principles of Genetics. 1997. Snustad et al.
From: Principles of Genetics. 1997. Snustad et al.
Linkage analysis of Elliptocytosis and Rh factor
From: Principles of Genetics. 1997. Snustad et al.
Summary
• LOD score analysis of linkage compares two possibilities: two loci are (a) linked at some specified recombination frequency and (b) are unlinked (rec. =50%). LOD = log10(probability of a genotype under a/ probability of the same genotype under b)
• Each progeny individual makes a positive or negative contribution to LOD.
• LOD scores of progeny from different families or crosses can be combined.
• A LOD score total of 3.0 or greater is generally considered strong evidence of linkage.
• Recombination frequency itself can be estimated by calculating LOD for different recombination values. The recombination frequency resulting in the maximum LOD score is treated as the most likely.