Anatomy & Neurobiology
ANA 812 Lab Session 7 - 2005
Muscle Tissue
Katherine FreedmanJamie Lewis
Jennifer MooreAndrea ThomasAndrew Usery
Anatomy & Neurobiology
Slide #29 Skeletal Muscle – Human (H&E) at 4X
• Cross section
• Longitudinal section
Anatomy & Neurobiology
Slide #29 Skeletal Muscle – Human (H&E) at 40X
• Perimysium• Endomysium• Peripheral Nuclei• Fascicle• Epimysium
4x
Anatomy & Neurobiology
Slide #29 Skeletal Muscle – Human (H&E) at 40X
• Endomysium
• Perimysium• Nuclei• Fascicle• A, I, & Z
bands
Anatomy & Neurobiology
Slide #73 Infant Tongue – Human (H&E) 40X
• Fibers oriented at right angles to each other
Anatomy & Neurobiology
Slide #20 Vertebra – Dog (H&E) 40X
• Skeletal Muscle
• Intrafusal Fibers
• Fluid Filled Space
• External Capsule (Connective Tissue)
Anatomy & Neurobiology
Slide #30:Cardiac muscle – human (H&E)
Intercalated discs
Bi-nucleated cell
Mono-nucleated cell
Striations
Branching cells
40x
Anatomy & Neurobiology
Slide #32:Cardiac muscle – rat (H&E)
Branching cell
Striations
Bi-nucleated cell
Mono-nucleated cell
40x
Anatomy & Neurobiology
Slide #37 Heart, Right Atrium - Human (H&E) 10x
• Longitudinal section
• Cross section
Anatomy & Neurobiology
Slide #37 Heart, Right Atrium - Human (H&E) 40x
• Longitudinal section
• Nuclei: one per cell and centrally located
• Intercalated discs
Anatomy & Neurobiology
Slide #38 Heart- Sheep (Masson’s Tri-Chrome) 4x
• Cross section
• Longitudinal section
Anatomy & Neurobiology
Slide #38 Heart- Sheep (Masson’s Tri-Chrome) 40x
• Cross section
• Longitudinal section
• Intercalated discs
Anatomy & Neurobiology
Slide #38 Heart- Sheep (Masson’s Tri-Chrome) 40x
• Purkinje fibers– Pale staining in center of
cytoplasm– Peripherally located
myofibrils– Round nuclei
Anatomy & Neurobiology
Slide #93 Colon-Monkey (H& E) 10X
•Smooth Muscle Cross-Section•Muscularis Externa
•Inner Layer (longitudinal)•Outer Layer (Cross-section)
Anatomy & Neurobiology
Slide #93 Colon-Monkey (H& E) 40X
•Cells packed closely together•No striations•Minimal Intracellular Space•Centrally Located, Ovoid Nuclei•Muscularis Externa
•Inner Layer (longitudinal)•Outer Layer (Cross-section)
Anatomy & Neurobiology
Slide #87 Gastroduodenal Junction-Human (PAS &
Hematoxylin) 2X
•External Lamina
•Smooth Muscle Cells
Anatomy & Neurobiology
Slide #87 Gastroduodenal Junction-Human (PAS &
Hematoxylin) 4X•Smooth Muscle Cells•External Lamina
•Glycoasminoglycans•Reticular Fibers
Anatomy & Neurobiology
Slide #87 Gastroduodenal Junction-Human (PAS &
Hematoxylin) 10X
•External Lamina
•Smooth Muscle Cells
Anatomy & Neurobiology
Similarities and Difference in Muscle Types (H & E) 4x
• Long Fibers Lack Branches
• Discernable Fiber Bundles
• Striations Lend Banded Appearance
• Fibers Branch and Anastomose
• Striations and Intercalated Discs are Indiscernible at Low Power
•Less Uniform Structure
•Lack Striations And Discs
•Tightly Packed Fibers
Skeletal Muscle Cardiac Muscle Smooth Muscle
Anatomy & Neurobiology
Similarities and Differences In Muscle Types (H & E) 40X
• Discernable A & I Bands
• Multinucleated Fibers
•Long Fibers that Do Not Branch or Anastomose
•Striations Apparent, But Less Defined
•Mono- or Bi- Nucleated Cells
•Fibers Branch and Anastomose with less linear structure
• Fibers Lack Striation
• Single, Centrally Located Nucleus
• Short, Spindle Shaped Cells that Do Not Branch
Skeletal Muscle Cardiac Muscle Smooth Muscle