![Page 1: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/1.jpg)
A Timed Presentation - do not click the mouse.
Approximate Run Time - 4 minutes
![Page 2: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/2.jpg)
Gel is 22.5 x 22.5 cm in size
Comb produces 28 wells
![Page 3: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/3.jpg)
![Page 4: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/4.jpg)
Uncut: DNA 18.75 μl10 x Buffer 15 μlWater 116.25 μl 6 x Loading Buffer 30 μl
30 μl/well – approximately 1.25 μg DNA/well
Single cut: DNA 37.5 μl10 x Buffer 22.5 μl
BamHI 15 μlWater 150 μl 6 x Loading Buffer 45 μl
30 μl/well – approximately 1.67 μg DNA/well
Bouble cut:DNA 37.5 μl10 x Buffer 22.5 μlBamHI 11.25 μlEcoRI 11.25 μ lWater 142.5 μl 6 x Loading Buffer 45 μl
30 μl/well – approximately 1.67 μg DNA/well
Each sample of DNA was incubated for 1 hour at 37°C.
Each sample and the ladder was then loaded into each well as outlined in the following slides.
pBabe/cpp326000 bp
BamHI
EcoRI
900 bp
5100 bp
pBabe plasmid donatedby Dr. Tang
![Page 5: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/5.jpg)
Lane 1
Lane 10
Lane 20
Lane 28
![Page 6: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/6.jpg)
![Page 7: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/7.jpg)
![Page 8: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/8.jpg)
![Page 9: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/9.jpg)
![Page 10: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/10.jpg)
![Page 11: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/11.jpg)
![Page 12: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/12.jpg)
![Page 13: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/13.jpg)
![Page 14: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/14.jpg)
![Page 15: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/15.jpg)
![Page 16: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/16.jpg)
![Page 17: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/17.jpg)
![Page 18: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/18.jpg)
![Page 19: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/19.jpg)
![Page 20: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/20.jpg)
![Page 21: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/21.jpg)
![Page 22: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/22.jpg)
![Page 23: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/23.jpg)
![Page 24: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/24.jpg)
![Page 25: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/25.jpg)
![Page 26: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/26.jpg)
![Page 27: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/27.jpg)
![Page 28: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/28.jpg)
![Page 29: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/29.jpg)
![Page 30: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/30.jpg)
![Page 31: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/31.jpg)
![Page 32: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/32.jpg)
![Page 33: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/33.jpg)
![Page 34: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/34.jpg)
2
3
1
BufferStain
![Page 35: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/35.jpg)
![Page 36: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/36.jpg)
27
17
LadderRows:
1
![Page 37: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/37.jpg)
![Page 38: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/38.jpg)
![Page 39: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/39.jpg)
![Page 40: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/40.jpg)
![Page 41: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/41.jpg)
![Page 42: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/42.jpg)
![Page 43: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/43.jpg)
![Page 44: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/44.jpg)
![Page 45: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/45.jpg)
![Page 46: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/46.jpg)
![Page 47: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/47.jpg)
![Page 48: A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes](https://reader037.vdocuments.mx/reader037/viewer/2022103122/56649ce45503460f949b186c/html5/thumbnails/48.jpg)
The SBI4U class ofHill Park Secondary School
wish to thank
Dr. D. Tang
(McMaster University/St. Joseph’s Hospital)and his lab technician
for donating the DNA, Restriction Enzymesand Ladder and for calculating the amounts
of material required to complete this procedure successfully.