Download - 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure
![Page 1: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/1.jpg)
5.1 Proteomics tools on ExPASy
![Page 2: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/2.jpg)
5.2 (Part 1) Primary, secondary, and tertiary protein structure
![Page 3: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/3.jpg)
5.2 (Part 2) Primary, secondary, and tertiary protein structure
![Page 4: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/4.jpg)
5.3 (Part 1) Protein structural profiles
![Page 5: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/5.jpg)
5.3 (Part 2) Protein structural profiles
![Page 6: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/6.jpg)
5.3 (Part 3) Protein structural profiles
![Page 7: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/7.jpg)
5.4 (Part 1) Two-dimensional polyacrylamide gel electrophoresis
![Page 8: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/8.jpg)
5.4 (Part 2) Two-dimensional polyacrylamide gel electrophoresis
![Page 9: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/9.jpg)
5.5 Affinity chromatography
![Page 10: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/10.jpg)
5.6 (Part 1) Basis of mass spectrometry peptide sequencing
![Page 11: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/11.jpg)
5.6 (Part 2) Basis of mass spectrometry peptide sequencing
![Page 12: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/12.jpg)
5.6 (Part 3) Basis of mass spectrometry peptide sequencing
![Page 13: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/13.jpg)
5.7 (Part 1) Peptide sequencing by tandem mass spectrometry
![Page 14: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/14.jpg)
5.7 (Part 2) Peptide sequencing by tandem mass spectrometry
![Page 15: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/15.jpg)
5.8 (Part 1) Quantitative proteomics using ICAT reagents
![Page 16: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/16.jpg)
5.8 (Part 2) Quantitative proteomics using ICAT reagents
![Page 17: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/17.jpg)
5.8 (Part 3) Quantitative proteomics using ICAT reagents
![Page 18: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/18.jpg)
5.8 (Part 4) Quantitative proteomics using ICAT reagents
![Page 19: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/19.jpg)
5.9 (Part 1) Antibodies and immunohistochemistry
![Page 20: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/20.jpg)
5.9 (Part 2) Antibodies and immunohistochemistry
![Page 21: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/21.jpg)
5.10 Protein microarrays
![Page 22: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/22.jpg)
5.11 Self-assembling NAPPA protein microarrays
![Page 23: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/23.jpg)
5.12 (Part 1) Yeast two-hybrid (Y2H) screens
![Page 24: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/24.jpg)
5.12 (Part 2) Yeast two-hybrid (Y2H) screens
![Page 25: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/25.jpg)
5.13 Applications of structural proteomics
![Page 26: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/26.jpg)
5.14 Flow diagram for solving a protein structure
![Page 27: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/27.jpg)
5.15 The Drosophila Engrailed homeodomain dimer bound to DNA
![Page 28: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/28.jpg)
5.16 (Part 1) Fold recognition
![Page 29: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/29.jpg)
5.16 (Part 2) Fold recognition
![Page 30: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/30.jpg)
5.17 Frequency of mutations recovered in “saturation” screens
![Page 31: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/31.jpg)
5.18 (Part 1) The three major types of mutagen
![Page 32: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/32.jpg)
5.18 (Part 2) The three major types of mutagen
![Page 33: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/33.jpg)
5.18 (Part 3) The three major types of mutagen
![Page 34: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/34.jpg)
5.19 (Part 1) F1 and F3 genetic screens
![Page 35: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/35.jpg)
5.19 (Part 2) F1 and F3 genetic screens
![Page 36: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/36.jpg)
5.20 (Part 1) Targeted mutagenesis
![Page 37: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/37.jpg)
5.20 (Part 2) Targeted mutagenesis
![Page 38: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/38.jpg)
5.21 (Part 1) Inhibitory RNA expression (RNAi)
![Page 39: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/39.jpg)
5.21 (Part 2) Inhibitory RNA expression (RNAi)
![Page 40: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/40.jpg)
5.22 Targeted loss- and gain-of-function genetics
![Page 41: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/41.jpg)
5.23 Deficiency mapping
![Page 42: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/42.jpg)
5.24 Transposon mutagenesis and enhancer trap screens
![Page 43: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/43.jpg)
5.25 Floxing mice
![Page 44: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/44.jpg)
5.26 Genetic fingerprinting
![Page 45: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/45.jpg)
Box 5.1 (Part 1) Hidden Markov Models in Domain Profiling
![Page 46: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/46.jpg)
Box 5.1 (Part 2) Hidden Markov Models in Domain Profiling
![Page 47: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/47.jpg)
Box 5.2 (Part 1) Biological Networks In Genome Science
![Page 48: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/48.jpg)
Box 5.2 (Part 2) Biological Networks In Genome Science
![Page 49: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/49.jpg)
Box 5.2 (Part 3) Biological Networks In Genome Science
![Page 50: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/50.jpg)
Box 5.2 (Part 4) Biological Networks In Genome Science
![Page 51: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/51.jpg)
Box 5.3 (Part 1) Transgenic Animals and Plants
![Page 52: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/52.jpg)
Box 5.3 (Part 2) Transgenic Animals and Plants
![Page 53: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/53.jpg)
Exercise 5.1 Structural annotation of a protein
![Page 54: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/54.jpg)
Exercise 5.2 (Part 1) Identification of a protein on the basis of a MS profile
![Page 55: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/55.jpg)
Exercise 5.2 (Part 2) Identification of a protein on the basis of a MS profile
![Page 56: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/56.jpg)
Exercise 5.3 Formulating a network of protein interactions
![Page 57: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/57.jpg)
Exercise 5.4 Designing a genetic screen
![Page 58: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure](https://reader036.vdocuments.mx/reader036/viewer/2022062518/56649e2b5503460f94b19c9f/html5/thumbnails/58.jpg)