4N6FLOQSwabs™ for DNA Collection at the Crime Scene
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Presentation Outline
Introduction
4N6FLOQ Swabs™ Overview
4N6FLOQ Swabs™ for Crime Scene
Ordering Information
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DNA Collection at the Crime Scene
“Having proper supplies on the scene—
and the knowledge of how to properly
use them—is critical to the collection
of usable DNA samples.”
Joseph Blozis – NYPD Detective Sergeant (Retired) Using DNA to Fight Property Crime, Evidence Technology Magazine, September-October 2012
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Critical DNA Evidence Supplies
Swabs
Swab pouches or tubes
Water vial
Plastic pipettes
Gloves
Envelope for transport
Evidence seal
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4N6FLOQSwabs™ Overview
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Excellent recovery of DNA
Maximum efficiency in collection capacity
Available with different anatomical and ergonomic design
Human DNA, Dnase and RNase-free certified
ETO-treated (Ethylene Oxide)
Pre-scored breaking point
Introducing the 4N6FLOQSwabs™
Patented technology to maximize DNA collection and elution efficiency
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Flocked Swab Swab Structure Comparison
Cotton Swab
2 km microfiber 6 m microfiber
Sample stays entrapped in the
fiber wand
Sample is released quickly and in
higher amounts
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4N6FLOQSwabs™ for Human Identification
Crime Scene Reference Samples
Designed to meet the needs of forensic and paternity laboratories
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4N6FLOQSwabs™ for Crime Scene
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4N6FLOQSwabs™ for Crime Scene
• Anatomically and ergonomically shaped • Optimized for challenging samples • Treated with antimicrobial agents • No need for drying
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Detect & Analyze Amplify Quantify
Workflow for Casework Samples
Extract Collect
GeneAmp® PCR System 9700 Cycler Veriti® 96-Well Thermal Cycler
PCR Amplification Kit
3130/3130xl Genetic Analyzer
3500/3500xL Genetic Analyzer
GeneMapper® ID-X software
Excellent profiles with limited or trace amount of DNA
4N6FLOQSwabs™
AutoMate Express™
PrepFiler™ kit Quantifiler® Duo
7500 Real-Time PCR System
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Applications Part Number 4N6FLOQSwabs™ Application
4479429 Regular head tip, in peelpouch, with Antimicrobial Action
Multi-use swab for collection of traces from crime scene. After collection the swab must be stored in a sterile microtube.
4479427 Regular head tip, in plain tube, with Antimicrobial Action
Multi-use swab the collection of traces from crime scene. After collection the swab can be stored in its own plastic tube.
4479438 Scalpel shape tip, in plain tube, with Antimicrobial Action
Multi-use swab with a scalpel head shape, designed for collection of traces in sharp corners. After collection the swab can be stored in its own plastic tube.
4479430 Regular mini head tip, in plain tube, with Antimicrobial Action
Multi-use swab with a thin head shape designed for collection of traces in narrow opening such as cracks or fissures. After collection the swab can be stored in its own plastic tube.
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Applications Part Number 4N6FLOQSwabs™ Application
4479435 Regular head tip, in plastic short tube, with Antimicrobial Action
Multi-use swab for collection of traces from crime scene. The shorter swab shaft and the wider tube diameter reduces the risks of sample loss.
4479434 Dual Swab Regular size tip in plain tube, with Antimicrobial Action
Dual-head swab for collection of traces from crime scene designed to facilitate sample retention. After collection the swab can be stored in its own plastic tube.
4479437 Subungual Shape, in plain tube, with Antimicrobial Action
Swab designed for collection of traces under the nails or narrow openings like metal watch bands. The shorter swab shaft and the wider tube diameter reduces the risks of sample loss.
4479428 Round Shape, in plain tube, with Antimicrobial Action
Swab designed for the collection of traces on rounded surfaces such as bottles. The shorter swab shaft and the wider tube diameter reduces the risks of sample loss.
4479441 Mini tip, in plain tube, with Antimicrobial Action
Multi-use swab with a thin head shape designed for collection of traces in narrow opening such as cracks or fissures. The shorter swab shaft and the wider tube diameter reduces the risks of sample loss.
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Collection Steps
1) PREPARATION Hold the plastic tube with both hands while wearing gloves; twist the black handle counterclockwise to break the seal, then remove the swab from the tube.
3) STORAGE Place the 4N6FLOQSwab inside the plastic storage tube. Avoid touching the inner sides of the tube with the swab. Seal the tube with a tampering control label and label the sample.
Insert swab in the tube Seal and label the tube
2) COLLECTION Wet samples: collect the sample by swabbing the area using the tip the 4N6FLOQSwab. Roll swab until the sample is completely collected. Dry samples: place one drop of sterile water (or phosphate buffer solution) on the side of the swab tip. Then swab the area by rotating the swab so that the entire surface of the swab is used. Reswab the area with a dry sterile swab1.
Wet sample
Rotate the swab Swab the area
1. Using DNA to Fight Property Crime, Evidence Technology Magazine, September-October 2012
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Tips for Improving DNA Sample Collection
SWABBING THE AREA • Excessive rolling may result in sample loss. • Avoid touching any other surface after the sample has been collected. • Maximize swab coverage: one swab for every 15 square centimeters of surface area1
IRREGULAR GRAINY SURFACE • Swab with the grain • Swab back and forth, rotating the swab surface to ensure that the sample area is not reused
USE OF 4N6FLOQSwabs™ IN PEEL POUCH • Insert the swab in a microtube • Break the shaft at the swab breaking point • Seal and label the tube Insert swab
in microtube Break Shaft Seal and label
SWAB MOISTENING • Avoid using too much water (∼30 µL) which may result in slow-drying swabs and diluted samples1
Using DNA to Fight Property Crime, Evidence Technology Magazine, September-October 2012
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Ordering Information
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4N6FLOQSwabs™ - Crime Scene 4N6FLOQSwabs™ Part Number Quantity
Regular head tip, in peelpouch, with Antimicrobial Action 4479429 Case of 100
Regular head tip, in plain tube, with Antimicrobial Action 4479427 Case of 100
Scalpel shape tip, in plain tube, with Antimicrobial Action 4479438 Case of 100
Regular mini head tip, in plain tube, with Antimicrobial Action 4479430 Case of 100
Regular head tip, in plastic short tube, with Antimicrobial Action 4479435 Case of 50
Dual Swab Regular size tip in plain tube, with Antimicrobial Action
4479434 Case of 100
Subungual Shape, in plain tube, with Antimicrobial Action
4479437 Case of 50
Round Shape, in plain tube, with Antimicrobial Action 4479428 Case of 50
Mini tip, in plain tube, with Antimicrobial Action 4479441 Case of 50
For Forensic or Paternity Use Only
The trademarks mentioned herein are the property of Life Technologies Corporation and/or its
affiliate(s) or their respective owners. FLOQSwabs and 4N6FLOQSwabs are trademarks of Copan Flock Technologies.
©Copyright 2012. Life Technologies Corporation. All rights reserved.