![Page 1: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/1.jpg)
S L I D E 1
3D Bioprinting of VesselsA new means to Improve patient Outcomes
John Geibel,MD,DSc,MSc,AGAFVice Chairman Department of SurgeryDirector of Surgical ResearchProfessor of Surgery and Cellular and Molecular PhysiologyAmerican Gastroenterological Association Fellow
![Page 2: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/2.jpg)
S L I D E 2
3D Bioprinting of Vessels, a new means to Improve patient Outcomes
• Disclosures– Organovo
![Page 3: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/3.jpg)
S L I D E 3
Three-Dimensional (3D) Printing
• Also known as additive manufacturing, refers to any of several processes involving sequential deposition of material to form a 3D objecthttps://en.wikipedia.org/wiki/3D_printing
https://chemical-materials.elsevier.com/the-world-of-3d-printing-in-2015/
![Page 4: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/4.jpg)
S L I D E 4
3D Bioprinting of Vessels, a new means to Improve patient Outcomes
• Are we ready for 3D printing in the 21st Century ?
![Page 5: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/5.jpg)
S L I D E 5
•Yes
![Page 6: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/6.jpg)
S L I D E 6
Bioprinting
• How Does it Work ?• Use Printer with 2 Print Cylinders
– Cells– Hydrogel
• Create 3D Model of Vessel
• Prepare Vessel for Implantation
![Page 7: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/7.jpg)
S L I D E 7
Schematic of the Process
![Page 8: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/8.jpg)
S L I D E 8
Creation of Rat Arterial Vessel: Elapsed time to Finished Print 3 Hours
![Page 9: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/9.jpg)
S L I D E 9
![Page 10: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/10.jpg)
S L I D E 10
Cross-Section of Print 36+ hours from Print
![Page 11: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/11.jpg)
S L I D E 11
Perfusion of New Print Technology
![Page 12: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/12.jpg)
S L I D E 12
Question of Tensile Strength
• Are the new prints able to withstand the pressures of aortic perfusion?
![Page 13: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/13.jpg)
S L I D E 13
![Page 14: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/14.jpg)
S L I D E 14
Rat Aorta
![Page 15: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/15.jpg)
S L I D E 15
Implanted full length graft
![Page 16: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/16.jpg)
S L I D E 16
Intestinal Repair with 3D Prints
![Page 17: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/17.jpg)
S L I D E 17
Larger ID multilayer robust print
• Print at 24 hours post print
![Page 18: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/18.jpg)
S L I D E 18
Process For Intestinal Patch
![Page 19: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/19.jpg)
S L I D E 19
![Page 20: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/20.jpg)
S L I D E 20
In Vivo 21 days Post Op
![Page 21: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/21.jpg)
S L I D E 21
30 Days Post Implantation of Patch
Implant Cross Section Sham Operated Cross Section
![Page 22: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/22.jpg)
S L I D E 22
Yale Instrumentation Initiative Help
• Critical Needs for your Instrumentation Work– Supplies to expand cell culture levels to move on to higher organisms– Acquisition of More incubators and TC hood to allow for more prints
• How could the Yale Instrumentation Initiative help your work
– Provide infrastructure to make facility available to more labs– Expand the printing facility to make it accessible to other labs/groups
• What can you offer to the Yale Instrumentation Community
– Consult on what is needed for successful 3D Bioprinting– Potentially print organoids for drug development using human cell
models– Work on designing Cartridges for 3D Organ replacement
• Pancreas• Liver• Kidney
![Page 23: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/23.jpg)
S L I D E 23
Acknowledgements
• Muyoka Maina(Yale)• Peter Geibel (Yale)• Brian Wengerter (Yale)• Gulus Emre (Yale)• Alan Dardik (Yale)• Hualong Bai (Yale)• Taras Lysyy (Yale)• Julian Campillo Luna(Yale)• Michael Shang(Yale)• Jack Gagne(Yale)• Organovo technical support team
![Page 24: 3D Bioprinting of Vessels - instrumentation.yale.edu](https://reader034.vdocuments.mx/reader034/viewer/2022051609/6280a137e4320e18054e22a1/html5/thumbnails/24.jpg)
S L I D E 24
Thank you!
• Questions?