Download - 2.7 Factors affecting enzyme action
2.7 Factors affecting enzyme action
Learning Objectives
• Learn how the rate of an enzyme controlled reaction is measured.
• Learn how temperature affects the rate of an enzyme controlled reaction.
• Learn how pH affects the rate of an enzyme controlled reaction.
• Learn how substrate concentration affects the rate of reaction.
How to measure the progress of enzyme-catalysed reactions...
• Generally, one of two ‘outcomes’ are measured over time:
- the formation of the products of the reaction.- the disappearance of the substrate.
Draw and explain the following graphs...Vo
lum
e of
oxy
gen
prod
uced
Time
Mas
s of s
tarc
h
Time
Enzyme-catalysed reaction in which oxygen is formed due to the
action of catalase on hydrogen peroxide.
Enzyme-catalysed reaction in which starch disappears due to the
reaction of amylase.
The Effect of Temperature on the Rate of an Enzyme-Controlled Reaction
The Effect Of pH
The Effect Of pH on the Rate of an Enzyme-Controlled Reaction
The Effect Of Changing Substrate Concentration
The effect of changing the amount of substrate on the rate of an enzyme-controlled reaction. As the amount of substrate increases from 0 to B, the rate of reaction increases. As the amount of substrate increases from B to C, the rate of
reaction does not increase any further.