Do Now What are the functions of these macromolecules:

1. Carbohydrates (polysaccharides)

2. Lipids

3. Proteins

4. Nucleic Acids

Biochemistry Classwork ActivitySection 1: (DO THIS WELL)On your paper, put together the correct monomers to create a:1.Polysaccharide2.Protein3.Lipid4.Nucleic acid

Draw the correct parts together. Write the name of the macromolecule (large compound) and the building blocks (monomers) that make it up

Sections 2 & 3 = directions on sheet

DO NOW1. Enzymes, hormones and cell receptors are

examples of which type of macromolecule?

2. How are enzymes, hormones and cell receptors different from one another?

**Remember all LAB corrections and LABS due THURSDAY for a GRADE!!

Enzymes & pH

http://phet.colorado.edu/sims/html/ph-scale/latest/ph-scale_en.html

Do Now

1. How is a cell receptor similar to an enzyme?

2. How is a cell receptor different from an enzyme?

3. Are enzymes organic? Why?

EnzymesHow can we break down a piece of pepperoni pizza?

What is the pizza broken down into?

Can those _______________ be synthesized into new molecules? How?

Chemical reactions with enzymes are used to break molecules down and…

Carbohydrate Carbohydrate Monosaccharide + Monosaccharide Monosaccharide + Monosaccharide

Lipid Lipid Fatty acid + Fatty acid Fatty acid + Fatty acid

Protein Protein Amino acid + Amino acid Amino acid + Amino acid

Nucleic Acid Nucleic Acid Nucleotide + Nucleotide Nucleotide + Nucleotide

Chemical reactions with enzymes are used to break molecules down and put them together!

Monosaccharide + Monosaccharide Monosaccharide + Monosaccharide Carbohydrate Carbohydrate

Fatty acid + Fatty acid Fatty acid + Fatty acid Lipid Lipid

Amino acid + Amino acid Amino acid + Amino acid Protein Protein

Nucleotide + Nucleotide Nucleotide + Nucleotide Nucleic Acid Nucleic Acid

Wait, what’s a chemical reaction?When one or more substances reacts to form new substances.

Na

Silver solid

+ Cl

Green gas

NaCl

Table Salt

Na Cl NaCl+

Reactants Products

The substances which react.

The substances which are produced.

Why do we need enzymes??Isn’t water all you need to break down & synthesize molecules?Lets take a look: (Think about these 2 questions while observing)http://www.lpscience.fatcow.com/jwanamaker/animations/Enzyme%20activity.html

1.Will molecules breakdown or synthesize without enzymes?

2.Why are enzymes important for chemical reactions?

Hmmmm…

1. Does a Jolly rancher or Starburst dissolve faster in your mouth or a glass of water?

2. Why might this happen?

+ H20

How could you speed up this chemical reaction?

Enzyme doing Hydrolysis

Do Now 1. Why are enzymes necessary for living organisms?

2. How are enzymes different from one another?

3. Can chemical reactions occur without enzymes? Explain.

**All missed, not turned in and failed labs will be a ZERO if not handed in or corrected by Thursday end of extra help!

EnzymesEnzymes:

Are organic catalysts that speed up chemical reactions

Catalyst – something that speeds up a reaction

Example: Carbon Dioxide and Water come together to make Carbonic Acid

– Happens in red blood cells, but only about 200 molecules of carbonic acid form an hour

– Add the enzyme carbonic anhydrase, and an estimated 600,000 molecules of carbonic acid are now formed every second!

EnzymesEnzymes are very specific.

Each enzyme only has one reaction it will help

Example: Lactose (a disaccharide), will only be broken down by the

enzyme lactaseMaltose will only be broken down by maltaseStarch (amylose) will only be broken down by amylaseProteins will only be broken down by protease (peptidase)

Wait a minute… what do you notice about many enzyme names?

Protease, amylase, maltase, lactase, lipase…

Using words alone, how can you tell which word is a sugar and which is an enzyme? (label the sugar and enzyme)

Amylase vs. Amylose

Why do enzymes only help catalyze (speed up) only one type of reaction?

Enzyme shape allows only certain reactants (substrates) to bind to the enzyme

– Substrate: the specific reactants that an enzyme acts on– Active site: the part of the enzyme where the substrate

connects. Specific to only one substrate! • Substrate – active site = like a lock and key!

Specific, Picky Enzymes(Definition of playing hard to get!)

Lets take a look at how enzymes are specific:http://www.lpscience.fatcow.com/jwanamaker/animations/Enzyme%20activity.html

When watching, keep in mind these questions:

1. How are the enzymes shown different?

**Remember: SUBSTRATE - ENZYME

Label your diagram

Enzyme (2x)Substrate Products

Reactants Active SiteEnzyme-Substrate Complex

Word Bank… don’t get used to this

2. Exit Question – Write a sentence or two stating the conclusion or relationship between the active site and the specific substrate.

3. Bonus question – can this enzyme be used again for the same reaction? For a different reaction?

1. Look at the active site. Did it change after the reaction?

Do NowDo Now (4)

1. Write sentences describing the relationship between: Active Site / Specific Substrate

Enzyme / Breaks down, Synthesis

Enzymes / “ASE”

Enzyme / Catalyst

Bonus Question

Example: Amylase only breaks down starch (amylose)– Which molecule is the enzyme and which is the

substrate?

Enzymes are Reused Over and Over and Over again

1. What type of reaction is this (break down or synthesis)?

2. What changed in this reaction?

3. What stayed the same?

Enzymes are Reused Over and Over and Over again

Your conclusion?

http://www.lpscience.fatcow.com/jwanamaker/animations/Enzyme%20activity.html

Enzymes = Specific, Picky and High Maintenance

The rate at which enzymes catalyze their reactions changes as the conditions inside the cell change!

Conditions that effect enzyme reaction rate are:1. Temperature 2. Relative concentrations of enzyme and substrate 3. pH (acidic, basic, neutral)

Temperature affects Enzyme Function

To cold = enzyme works to slowTo hot = enzyme begins to denature!

– Denature = proteins begin to break apart at high temperatures changing the active site!!

– Why is this bad?

Every enzyme has an optimal temperature it works best at!

Why is a very high fever considered dangerous where you have to go to the hospital?

http://www.lpscience.fatcow.com/jwanamaker/animations/Enzyme%20activity.html

Do Now 6

pH affects Enzyme Function

What is pH?– Measures how acidic or basic a solution is

Acids – compounds that release H+ ions in solution (corrosive)Bases – compounds that remove H+ from solution

pH scale - measures how acidic a solution is– pH = usually between 0 -14 – pH 0 = very acidic (high H+ concentration)– pH 14 = very basic (low H+ concentration)– pH 7 = neutral (not basic or acidic)

pHWhat is basic and what is neutral?

What is the pH of human blood?

What is the pH of water?

Acids vs. Bases

How do we test the pH of a solution?

Using litmus paper!

Litmus paper turns this color when:1. Acidic2. Basic

What color did the litmus paper turn with:•Lemon juice – •Bleach –

pH affects Enzyme FunctionpH affects Enzyme FunctionJust like temperature, enzymes (proteins) will start to breakdown permanently if the pH is not what the enzyme needs!

1.Do all enzymes work best at the same pH?

2.What is the optimum rate for Gastric Protease in graph B?

BMost enzymes work best in neutral solutions

pH affects Enzyme FunctionpH affects Enzyme Function• Humans need to keep their pH within a very

narrow range around neutral (pH 7.0). – Some organisms need different pH ranges

• The azalea plant thrives in acidic (4.5) soil and has a microorganism called Picrophilus that survives best at an extremely acidic pH of 0.7.

Enzyme-Substrate ConcentrationEnzyme-Substrate ConcentrationEnzymes can only catalyze so fast.

– If you keep adding substrates, the enzymes reaction rate will go up

– At a certain point, enzymes can not work any faster and the rate will not increase! It will level off!

Why does the reaction rate line level-off or flatten out?

Summary of Diagrams you will see

Questions 1. Do all enzymes react best at the same temperatures?

2. Do all enzymes react best at the same pH?

3. What is pH a measure of?

4. What happens to an enzyme when it is to hot or acidic?