DO NOW: 1. Hand in the field trip brainstorming you wrote on

Friday.2. Take out your microscope Green packets from

last week.3. In your NOTES section of your binder, please list

EIGHT differences between these two pictures.

Stare at the red dot on the girl’s nose for 30 seconds.Turn your eyes to a plain surface.Blink repeatedly and quickly.

Using a Compound Light Microscope Directed Reading

Using the Microscope1.) Ocular lens (eyepiece):

2.) Low power objective:

3.) High power objective:

magnifies the image 10 x.

magnifies the image 10 x.

magnifies the image either40x or 43 x.

Using the Microscope4.) Revolving Nosepiece:

5.) Body Tube:

6.) Coarse Adjustment Knob:

holds the objective and can beturned to change from one magnification to the other.

maintains the correct distancebetween the lens and objectives

Moves the body tubeup and down to allow focusing of the

image

Using the Microscope7.) Fine Adjustment Knob:

8.) Stage:

9.) Stage Clips:

Moves the body tube slightly to bring the image into sharper

focussupports the slide

Hold the slide in place for viewing

Using the Microscope10.) Diaphragm:

11.) Light:

12.) Arm:

Controls the amount of light comingthrough the stage

A light source provides this for viewingthe slide

supports the body tube

Using the Microscope13.) Base:

14.) Inclination Joint :

supports the microscope

Allows the upper part of themicroscope to be tilted back to view the

specimenwhile seated

Proper Use of the Compound Light Microscope

1.) Describe how you would carry a microscope

 

Use both hands - one hand beneath the base - other hand to hold the armHold the microscope close to your body

Proper Use of the Compound Light Microscope

2.) How many centimeters should the microscope be from the edge of the table?

3.) What power objective should you ALWAYS begin working with?

 

5 cm

The low-power objective lens

Proper Use of the Compound Light Microscope

4.) If your microscope is not in low power, what should be adjusted?

 5.) Describe what you do with your prepared slide next

 

The revolving nosepiece

Place it over the hole in the stage and secure the slide with stage clips.

Proper Use of the Compound Light Microscope

6.) When looking through the ocular lens, describe why you adjust the diaphragm

7.) Once you are looking at the stage from eye level what you should do next?

 

To adjust the amount

Adjustment to lower the objective until it almost

of light coming through the stage.

Slowly turn the coarse

touches the slide.

Proper Use of the Compound Light Microscope

8.) What should you NOT allow the objective to do?

10.) What is responsible for raising the low-power objective?

11.) What does raising the low-power objective do?

Touch the slide!

The coarse adjustment knob

Allows the image to be in focus

Proper Use of the Compound Light Microscope

12.) Complete the sentence: “Always focus by away from the slide. Never focus ”.

13.) After the course adjustment knob initials focuses the image, describe what the fine adjustment knob does

the objectiveobjective downward.

It sharpens the focus.

raisingthe

Proper Use of the Compound Light Microscope

14.) When looking in the microscope, describe where the image should be located before switching to high power

15.) Once you have switched the microscope to high power, explain what you do with the fine and course adjustment knobs

Exactly in the center of your field of vision

Focus the image, using ONLY thefine adjustment. NEVER use the coarse

adjustmentat high power.

Proper Use of the Compound Light Microscope

16.) When you are all DONE with the microscope, what should you do next before you leave the classroom? You must remove the slide. Clean the

ocular lensand objective lenses with lens paper. Return the

Should use both hands to carry the microscope.

Microscope to its storage area. Remember, you

Making a Wet Mount Slide1.) When cleaning a glass slide, you should use sand paper to clean the slide and coverslip.When cleaning a glass slide, use lens paper to clean the slide and a coverslip.

Place the specimen you wish to observe in the center of the slide.

Making a Wet Mount Slide2.) Place the specimen you want to observe to the left of the slide.

Place one drop of water on the specimen using the medicine dropper.

Making a Wet Mount Slide3.) Place 5 drops of water on the specimen using the medicine dropper.

Hold the coverslip at the edge of the water at a 45 degree angle to the slide. Make sure the water runs along the edge of the coverslip.

Making a Wet Mount Slide4.) Hold the coverslip at the edge of the water at a 90 degree angle to the slide. Make sure the water runs along the middle of the coverslip.

Lower the coverslip slowly to avoid trapping air bubbles.

Making a Wet Mount Slide5.) Quickly lower the coverslip to trap as many air bubbles as possible.

Water might evaporate from the slide as you work. Add more water to keep the specimen fresh.

Making a Wet Mount Slide6.) Water might evaporate from the slide as you work so do not add any more water to keep the specimen fresh.

Place the tip of the medicine dropper next to edge of the coverslip. Add a drop of water.

Making a Wet Mount Slide6a.) Place the tip of the medicine dropper next to edge of the coverslip and add 4 drops of water.

Remove excess water from the slide by using the corner of a paper towel as a blotter.

Making a Wet Mount Slide6b.) Add excess water from the slide by using the corner of a paper towel as a blotter.

DO NOT lift the coverslip to add or remove water.

Making a Wet Mount Slide6c.) You must lift the coverslip to add or remove water.