Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

SAGAR KISHOR SAVALE 1

Dissolution: How to Calculate Dissolution Calculation in Excel Sheet

Step 1: Std. Calibrating curve

10 mg drug was dissolved in 100 ml pH 6.8 Phosphate Buffer, to prepared 100 ug/ml solution

0.2 ml solution is diluted with 10 ml Buffer 2 µg/ml solution

0.4 ml solution is diluted with 10 ml Buffer 4 µg/ml solution

0.6 ml solution is diluted with 10 ml water 6 µg/ml solution

0.8 ml solution is diluted with 10 ml Buffer 8 µg/ml solution

1.0 ml solution is diluted with 10 ml Buffer 10 µg/ml solution

1.2 ml solution is diluted with 10 ml Buffer 12 µg/ml solution

(Above all solutions are 10 µg/ml solution)

Step 2: Take absorbance of all solution and Plot graph in MS Excel.

Step 3: After taking absorbance, to plot graph in Excel, Click Insert Chart Scatter

Select the Graph

Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

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Step 3: To give Chart Tile or Axis Tile

Absorbance of Prepared

Concentrations (10 µg/ml)

Scatter

Inserts

Chart

Select Graph

Generated Graph

Click here to give chart title and Axis title

Title

Axis Title

Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

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Step 4: Generate the equation

To select line of the graph write click click Add Trendline

To click Format Trendline

Tic – Display equation on chart

Tic – Display R – Squared value on chart

Generate Equation

Y = mx + c

Where, Y = Absorbance,

m= Slope or gradient,

x = Concentration,

c = Y intercept,

To select line of graph

Write click

Add Trendline

Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

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To click Format Trendline

This winow is open at write side of the

computer screen

Tic – Display equation on chart

Tic – Dispay R squared on chart

Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

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Step 5: To Prepared Dissolution sheet

Time Abs Conc

µg/ml DF

Conc

µg/ml

Conc

mg/ml

Conc

mg/5ml

Conc

mg/900ml CDR %CDR

0 0 0 0 0 0 0 0 0 0

First Reading is always zero

Time: Time for dissolution is vary dosage from to dosage from.

Abs.: Taking solution after suitable time interval, to take absorbance of this solution, if absorbance is

more than 1, to dilute the solution.

Conc µg/ml: 1. To calculate Conc µg/ml with the help of Y = mx + c (Equation: std.

calibrating curve) this equation,

2. To convert this equation in x = Y-c / m (Y = Abs.)

DF: dilution factor is indicate number time to dilute the given solution (10, 100 and 1000).

Conc µg/ml: DF * Conc µg/ml to get Conc µg/ml.

Generate this equation

This winow is open at write side of the

computer screen

Dissolution – How to Calculate Dissolution Calculation in Excel Sheet

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Conc mg/ml: Conc µg/ml / 1000 to get Conc mg/ml.

Conc mg/5ml: Conc mg/ml * 5 to get Conc mg/5ml.

Conc mg/900ml: Conc mg/ml * 900 to get Conc mg/900ml.

CDR (cumulative drug release): to select reading in below the zero in CDR column *

First reading 0 in Conc mg/900ml to get CDR.

%CDR: (% cumulative drug release): (CDR*100) / Labelled Claim of drug to get

%CDR.

IMPORTANT NOTE:

1. In every calculation in Excel sheet, “Always starting the calculation with = this sine

(equal to sine)”, if you’re not used this sine calculation was not done.

2. After complete all calculation to press ENTER button to give your reading.

Mr. Sagar Kishor Savale

[Department of Pharmaceutics]

[R. C. Patel Institute of Pharmaceutical Education and Research Shirpur, 425405, MS, India]

avengersagar16@gmail.com

Always starting calculation with = this sine