discover the uniprot blast tool. murcia, february, 2011protein sequence databases customize the...
TRANSCRIPT
![Page 1: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/1.jpg)
Discover the UniProt Blast tool
![Page 2: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/2.jpg)
![Page 3: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/3.jpg)
![Page 4: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/4.jpg)
![Page 5: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/5.jpg)
Murcia, February, 2011Protein Sequence Databases
Customize the BLAST results
![Page 6: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/6.jpg)
Look for the local alignment
![Page 7: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/7.jpg)
Highlight important positions within the alignment according to the sequence annotation
The metal binding sites are conserved
![Page 8: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/8.jpg)
HBB_HUMAN
![Page 9: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/9.jpg)
Discover the UniProt Align tool
![Page 10: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/10.jpg)
1- Select sequences of interest… (from Blast results for example)
2 - Align…
![Page 11: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/11.jpg)
Add the ‘query’ sequence(HBB_HUMAN)
![Page 12: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/12.jpg)
Conservation of the metal-binding site….
Highlight important positions within the alignment according to the sequence annotation
![Page 13: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/13.jpg)
Compare the Blast
@ UniProt and
@ NCBI
![Page 14: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/14.jpg)
Protein Sequence Databases
The ‘alternative’ sequence(s)
not ‘directly available’ for a lot of tools, including protein identification tools, Blast, depending on the server !….
Murcia, February, 2011
![Page 15: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/15.jpg)
Blast P04150 against Swiss-Prot / homo sapiens @ UniProt
Isoform sequences
Murcia, February, 2011Protein Sequence Databases
![Page 16: Discover the UniProt Blast tool. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results](https://reader030.vdocuments.mx/reader030/viewer/2022032709/56649ea35503460f94ba7f99/html5/thumbnails/16.jpg)
Protein Sequence Databases
Blast P04150 against Swiss-Prot / homo sapiens @ NCBI
The isoform sequences (from Swiss-Prot) are not present in the NCBI protein database !The .x number (P06401.4) correspond to the version number of the sequence…not to an alternatively spliced sequence !
Murcia, February, 2011