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Figure S1. Growth of A. niger AXP8-1 (padA1+) (), AXP6-2.21a (padA1) () and AXP6-2.21aRa (padA1+, restored strain) (▼). ACM (pH 4.0) was inoculated with 104 conidia/ml and the cell dry weight yield (g/l) was recorded. Results presented are from independent cultures (n = 3 for each)
Figure S2. Decarboxylation of sorbic acid by A. niger AXP8-1 (padA1+), AXP6-2.21a (padA1) and AXP6-2.21aRa (padA1+, restored strain). Results presented are from independent cultures (n = 2 for each)
AXP8-1 AXP6-2.21a AXP6-2.21aRa
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BY4741 (wt) Y05833 VTU-padA1
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Figure S3. The decarboxylation of sorbic acid to 1,3-pentadiene by S. cerevisiae. By4741 = the wild-type (PAD1+), Y05833 = pad1, and VTU-padA1 = Y05833 (pad1) expressing the A. niger padA1 under the control of the ADH1 promoter