Data file

imagination at work

High-throughput process development

GE HealthcareLife Sciences

28-9258-39 AF

PreDictor™ 96-well filter plates and Assist softwarePreDictor 96-well filter plates (Fig 1) are prefilled with GE Healthcare Life Sciences BioProcess™ chromatography media (resins). PreDictor plates support high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions, either in a manual or in an automated workflow. Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation. Data generated using PreDictor plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening of process conditions.

Using PreDictor plates and Assist software shortens time-to-clinic and increases productivity in the process development lab by:

• Reduced experimental time: the time-scale for performing screening experiments can be reduced from weeks to hours

• Low sample consumption: new format significantly reduces sample consumption

• Increased process understanding: exploring an enlarged experimental space allows for a significant increase in process understanding

High-throughput process development for increased process understandingDevelopment of robust purification processes requires significant time and resources. The Quality by Design initiative by the FDA may put additional demands on process development work because a higher degree of process understanding is needed. This understanding can be obtained if a larger experimental space is investigated and/or more detailed studies are performed. Employing high-throughput techniques for process development aids in addressing these challenges.

Fig 1. PreDictor 96-well filter plates.

HTPD is a way of working that shortens development time and increases the amount of information available during early process development, while keeping sample consumption low. Chromatographic conditions are evaluated in a parallel manner using 96-well filter plates. As a result, a large number of experimental conditions may be evaluated simultaneously. This allows screening of a large experimental space to identify the sub-space that is favorable with respect to one or several defined responses. Once this sub-space has been found, optimization and scale-up may be done on columns and ÄKTA™ systems. The complete conceptual workflow is shown in Figure 2.

Fig 2. Conceptual visualization of a workflow for process development. Parallel screening using PreDictor plates or PreDictor RoboColumn units makes it possible to explore a large experimental space for best conditions (left). Once these have been identified, fine-tuning and verification are carried out on columns and ÄKTA systems (middle). Finally, scale-up is done under conditions in which a robust production scale process can be operated (right).

PreDictor prefilled 96-well filter plates, Assist software, and PreDictor RoboColumn™ for

screening of conditions

ÄKTA avant systems, UNICORN™ 6, HiScreen™, and

HiTrap™ columns for optimization

ReadyToProcess™ platform and AxiChrom™ columns for

scale-up and production

Scale-up

Total characterization space Detailed studies to define design space

Area for further optimization

2 28-9258-39 AF

HTPD workflowThe HTPD workflow consists of the following steps:1. Plan: systematic approach for experimental set-up.2. Perform: testing of various experimental conditions in a

parallel manner.3. Analyze: high-throughput analysis.4. Evaluate: data analysis and evaluation.

An experimental set-up should take advantage of the possibility to test many different conditions (factors) simultaneously. Preferentially this is done by using design-of-experiment (DoE), which employs statistics to identify and define the factors having the greatest impact on the process/product. This involves construction of a carefully selected set of experiments in which all relevant factors are varied and evaluated simultaneously to maximize the information gained. However, other experimental set-ups may be used as well.Before start, the experimental objectives should be defined.

These may include:

Screening• Which factors are most important (i.e., have significant

effect on the response)?• What factor range should be explored?

Optimization• What is the optimal combination of settings for the

important factors?• If there is more than one response, is it possible to find

common factor settings that satisfy all responses, or is a compromise needed?

Robustness testing• Is the process robust (i.e., unaffected by typical process

variations)?• How much will the product specifications vary due to

process variations?

It is important to define the factors and responses to be studied. The selection of analytical techniques for the responses will affect the amount of sample needed for each experiment. We recommend replicates to allow for outlier analysis. The number of factors and the number of replicates will influence the plate set-up. Assist software suggests plate layouts for different experimental designs.

Plan: systematic approach for experimental set-up

Perform: testing of various experimental conditions in a parallel mannerThe 96-well approach allows for high-throughput screening of a large number of experimental conditions. PreDictor plates are prefilled with different GE Healthcare Life Sciences BioProcess chromatography media. Experiments are performed in batch mode which eliminates errors induced by non-optimal formats or packing procedures. The principle is shown schematically in Figure 3. Removal of liquid through the bottom filter is done by either centrifugation or vacuum filtration.

Fig 3. A batch uptake experiment occurring in the wells of PreDictor plates. The steps in PreDictor plate experiments are the same as in a typical chromatography experiment: equilibration, sample loading, wash, and elution.

The number of samples to analyze in the HTPD workflow will be numerous. Therefore, high-throughput analytical methods are preferred if available. In some cases, simple A280 measurements may be sufficient during the initial screening step.

During the initial screening phase, analyses should be limited to the most critical responses. Based on these results, selected conditions can be chosen for responses that require more advanced analytical methods.

Analyze: high-throughput analysis

Data are analyzed and evaluated by using traditional evaluation methods or dedicated software tools. Assist software supports the management and evaluation of data generated with PreDictor plates. Based on the results, further investigation in HTPD mode may be relevant before moving to column verification and fine-tuning.

Evaluate: data analysis and evaluation

Mediain well

Wash/Equilibration

Waste

Incubation

Sampleaddition

Wash1–3 times

Elution1–3 times

Vacuum filtration or centrifugationVacuum filtration or centrifugation

Analysis

Mixing Mixing Mixing Mixing

The theoretical basis for batch uptake – In a typical adsorption process,a mass transfer mechanism (responsible for protein transport) and ligand selectivity are independent of the mode of operation (i.e., are the same whether they occur in a batch system or packed column). If a column is approximated by a cascade of hypothetical stages (theoretical plates) where a separation occurs, a single well in the filter plate can be seen as a single stage in a cascade.

In a chromatography column, any separation taking place in a single stage is further magnified in the next stage in the series. Therefore, as long as a difference in adsorption capacities/rates for different constituents of a sample can be quantified in a single well, the results obtained using PreDictor plates correlate well with the same separation occurring in a column.

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Applications

PreDictor plates can be used to screen different parts of the chromatographic cycle, for example determination of binding, wash, and elution conditions. This can be done for different chromatography media simultaneously using dedicated screening plates, or more thoroughly using plates with a single medium. Regardless of which, different applications will require different amounts of chromatography media in the wells (see “PreDictor plate selection” and Table 3).

Determination of absolute amounts in the lower concentration range may be difficult since it cannot be excluded that, under unfavorable conditions, non-specific adsorption to the plate may occur.

Screening of chromatographic conditions

Adsorption isotherms can also be determined in PreDictor plates. In short, the adsorption isotherm describes the relation between the concentrations of protein(s) in the liquid and solid phases at equilibrium under a given set of experimental conditions. This helps to understand and correctly describe what happens during protein uptake under different conditions. Dedicated PreDictor plates are available to facilitate experimental setups.

Application examples are described in references that are found under the “Related literature” section.

Determination of adsorption isotherms

PreDictor product characteristics

PreDictor plates are disposable, 96-well filter plates made of polypropylene and polyethylene. Each well has a total volume of 800 μL, and each plate is prefilled with a defined amount of chromatography medium per well. Table 1 summarizes the characteristics of PreDictor filter plates. For each chromatography medium, different plates with different volumes of media are available to fit various applications (Tables 2 and 3). The plates are sealed at the top and bottom at delivery. Plates are supplied in packs of four, which is sufficient to perform 128 tests in a study measured as triplicates. For larger studies, it is preferable to use PreDictor plates from the same lot.

96-well filter plates

Table 2 lists chromatography media that are available in PreDictor plates. For details on the different media, see corresponding data files listed under “Related literature”. Recommendations on which plate to use are given in Table 3.

Prefilled chromatography media

Table 1. PreDictor plate characteristics

Plate size 127.8 × 30.6 × 85.5 mm (according to ANSI/SBS 1–2004, 3–2004 & 4–2004 standards)

Material Polypropylene (PP) and Polyethylene (PE)Number of wells 96Well volume 800 µLWorking volume/well when incubating on a microplate shaker

100 to 300 μL*

Volume sedimented medium/well

2, 6, 20 or 50 μL. For PreDictor isotherm plates different in different wells: 2, 4, 6, 8, 20 and 50 μL

Medium suspensions in total volumes

200 μL for 2 μL sedimented medium/well500 μL for 6, 20, and 50 μL sedimented medium/wellFor PreDictor isotherm plates:– 500 μL for 50 μL sedimented medium/well– 200 μL for 20 μL sedimented medium/well– 500 μL for 8 μL sedimented medium/well– 375 μL for 6 μL sedimented medium/well– 250 μL for 4 μL sedimented medium/well– 125 μL for 2 μL sedimented medium/well

Storage solution PreDictor Capto™ S, Capto SP ImpRes, Capto MMC ImpRes, SP Sepharose™ Fast Flow, CIEX1 screening, Capto S isotherm, andSP Sepharose Fast Flow isotherm: 20% ethanol + 0.2 M sodium acetateAll other PreDictor plates: 20% ethanol

Recommended storage temperature

PreDictor MabSelect™, MabSelect SuRe™, MabSelect SuRe LX, MabSelect Xtra™, and Capto L:2°C to 8°CAll other PreDictor plates: 4°C to 30°C

Working temperature 4°C to 30°C (all plates)Centrifugation recommended 300 to 500 × g (sample dependent)Centrifugation maximum 700 x gVacuum pressure recommended –0.15 to –0.3 bar (sample dependent)Vacuum pressure maximum -0.5 barMicroplate shaker mixing speed

1100 rpm with 3 mm circular centripetal movement or sufficient mixing to maintain slurried chromatography medium in wells

Bar coded Placed on one of the short ends of the filter plate and containing:Article numberLot numberIndividual identification number

1 CIEX = cation exchange

* The lower volume in this interval indicates the working volume needed for effective mixing of sample/liquid on microplate shaker. The upper limit is the limiting volume for avoiding cross contami-nation between wells during mixing on a microplate shaker without sealing the top of the filter plate.

Note: The volume and the amount of protein needed for analysis are also to be taken into consider-ation.

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For optimal results, different applications and samples will require that the correct type of plate is used. Different PreDictor plates are therefore available to support different types of studies.

Different PreDictor plates

1. Single medium plates• For binding, wash or elution studies on a single

chromatography medium.• Same volume in all wells on a plate.• For each chromatography medium 2 to 3 different single

medium plates available, each with a different medium volume per well. Select plate depending on type of study (see Table 3).

2. Media screening plates• For binding, wash or elution studies on multiple

chromatography media.• Four types of plates are available:

- Anion (Capto Q, Capto DEAE, Q Sepharose Fast Flow) and multimodal anion (Capto adhere, Capto adhere ImpRes) exchanger media plates.

- Cation (Capto S, SP Sepharose Fast Flow) and multimodal cation (Capto MMC, Capto MMC ImpRes) exchanger media plates.

- HIC screening high hydrophobicity media plate (Phenyl Sepharose 6 Fast Flow (low sub), Capto Butyl, Phenyl Sepharose 6 Fast Flow (high sub), and Capto Phenyl (high sub).

- HIC screening low hydrophobicity media plate (Butyl-S Sepharose 6 Fast Flow, Octyl Sepharose 4 Fast Flow, Butyl Sepharose 4 Fast Flow, and Capto Octyl).

• For each plate type, two different media screening plates are available, each with different media volumes per well. Select plate depending on type of study (see Table 3).

3. Adsorption isotherm plates• For binding studies performed under equilibrium conditions

to obtain fundamental thermodynamic understanding of the adsorption process.

• Contains a single medium in all wells but with different volumes in different wells. This design allows simple and rapid construction of isotherms since different phase ratios are obtained without sample manipulations.

OperationThe standardized 96-well plate format gives considerable flexibility regarding mode of operation. PreDictor plates can be used in automated workflows using robotic systems, or operated manually using multi-channel pipettes. Removal of liquid can be made by either centrifugation or vacuum. Recommendations on centrifugation speed or vacuum filtration are given in Table 1.PreDictor plates and the chromatography media are compatible with all solutions commonly used in purification of biopharmaceuticals. Repeated loadings and long incubation times using buffers or samples containing certain detergents may induce some leakage through the filter. Recommendations to minimize leakage when working with detergents are given in the PreDictor plate Instructions.

PreDictor plate selectionTable 2. Available PreDictor plate products

Product

Singlemedium

plate

Chromatographymedium volume

per well* (μL)PreDictor Capto Q Yes 2†, 20 or 50PreDictor Capto S Yes 2, 20 or 50PreDictor Capto DEAE Yes 2, 20 or 50PreDictor Capto MMC Yes 6†, 20 or 50PreDictor Capto adhere Yes 6, 20 or 50PreDictor Capto MMC ImpRes Yes 6 or 20PreDictor Capto adhere ImpRes Yes 6 or 20PreDictor Capto Q ImpRes Yes 6 or 20PreDictor Capto SP ImpRes Yes 6 or 20PreDictor MabSelect SuRe LX Yes 6, 20 or 50PreDictor Q Sepharose Fast Flow Yes 6, 20 or 50PreDictor SP Sepharose Fast Flow Yes 6, 20 or 50PreDictor MabSelect Yes 6, 20 or 50PreDictor MabSelect SuRe Yes 6, 20 or 50PreDictor MabSelect Xtra Yes 6, 20 or 50PreDictor Capto L Yes 6, 20 or 50PreDictor Butyl Sepharose 4 Fast Flow Yes 6 or 50PreDictor Butyl-S Sepharose 6 Fast Flow Yes 6 or 50PreDictor Capto Butyl Yes 6 or 50PreDictor Capto Octyl Yes‡ 6 or 50PreDictor Capto Phenyl (high sub) Yes 6 or 50PreDictor Octyl Sepharose 4 Fast Flow Yes 6 or 50PreDictor Phenyl Sepharose 6 Fast Flow (high sub) Yes 6 or 50PreDictor Phenyl Sepharose 6 Fast Flow (low sub) Yes 6 or 50PreDictor HIC Screening High Hydrophobicity§ No 6 or 506

PreDictor HIC Screening Low Hydrophobicity¶ No 6 or 507

PreDictor AIEX1 Screening** No 2/6 or 20PreDictor CIEX Screening†† No 2/6 or 20PreDictor adsorption isotherm plate No Different medium

volume in differentwells(2, 4, 6, 8, 20 and 50)

* Note that the total medium suspension volume per well is larger than the chromatography medium volume. The total medium suspension volume per well is different depending on chromatography medium volume in the well. For total medium suspension volume per well, see Table 1.† The plates with 2 and 6 μL are developed for the same application. Different volumes are used based on the properties of the media: 2 μL is sufficient for the high-capacity ion exchangers, while for the other media 6 μL is required for optimal results.‡ At this time, Capto Octyl is part of the CDM portfolio, and is produced upon customer order.§ PreDictor HIC screening high hydrophobicity contains following chromatography media: Phenyl Sepharose 6 Fast Flow (low sub), Capto Butyl, Phenyl Sepharose 6 Fast Flow (high sub) and Capto Phenyl (high sub).¶ PreDictor HIC screening low hydrophobicity contains following chromatography media: Butyl-S Sepharose 6 Fast Flow, Octyl Sepharose 4 Fast Flow, Butyl Sepharose 4 Fast Flow and Capto Octyl.

** PreDictor AIEX screening plate 2μL/6μL contains the following chromatography medium volumes per well: Capto Q 2 μL, Capto DEAE 2 μL, Q Sepharose Fast Flow 6 μL and Capto adhere 6 μL. PreDictor AIEX screening plate 20 μL contains 20 μL per well of the corresponding media.†† PreDictor CIEX screening plates 2μL/6μL contains the following chromatography medium volumes per well: Capto S 2 μL, SP Sepharose Fast Flow 6 μL and Capto MMC 6 μL. PreDictor CIEX screening plate 20 μL contains 20 μL per well of the corresponding media.1 AIEX = anion exchange

The plates can be divided into three main categories:

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Screening plates: for screening of binding conditions

Medium volume per well (μL) AIEX screening plate CIEX screening plateHIC low hydrophobicity

screening plateHIC high hydrophobicity

screening plate

2 or 6 ++ ++ ++ ++20 or 50 - - - -

Screening plates: for screening of wash/elution conditions

Medium volume per well (μL) AIEX screening plate CIEX screening plateHIC low hydrophobicity

screening plateHIC high hydrophobicity

screening plate

2 or 6 - - - -20 or 50 ++ ++ ++ ++

Isotherm plates: for determination of adsorption isothermsMedium volume per well (μL) Different in different wells: 2, 4, 6, 8, 20 and 50Media available‡ Capto Q, Capto S, Capto DEAE, Capto MMC, Capto adhere, Q Sepharose Fast Flow, SP Sepharose Fast Flow, MabSelect,

MabSelect SuRe, and MabSelect XtraNote: only one type of medium per plate

++ First choice + Possible - Not recommended NA Product not available‡ Additional media available on request.

Table 3. PreDictor plate selection guide

Single medium plates: for screening of binding conditions

Medium volumeper well (μL) Capto Q Capto S

CaptoDEAE

CaptoMMC

Captoadhere

Capto MCCImpRes

Capto adhereImpRes

Capto SPImpRes

Capto QImpRes

Q SepharoseFast Flow

SP SepharoseFast Flow

MabSelectFamily Capto L

2 ++ ++ ++ NA NA NA NA NA NA NA NA NA NA6 NA NA NA ++ ++ ++ ++ ++ ++ ++ ++ ++ ++20 - - - - - - - - - - - - -50 - - - - - NA NA NA NA - - - -

Medium volumeper well (μL)

Butyl-SSepharose 6

Fast Flow

OctylSepharose 4

Fast Flow

ButylSepharose 4

Fast Flow

PhenylSepharose 6

Fast Flow (low sub) Capto Octyl Capto Butyl

PhenylSepharose 6

Fast Flow (high sub)Capto Phenyl

(high sub)

6 ++ ++ ++ ++ ++ ++ ++ ++50 - - - - - - - -

Single medium plates: for screening of wash/elution conditions

Medium volumeper well (μL) Capto Q Capto S

CaptoDEAE

CaptoMMC

Captoadhere

Capto MCCImpRes

Capto adhereImpRes

Capto SPImpRes

Capto QImpRes

Q SepharoseFast Flow

SP SepharoseFast Flow

MabSelectFamily Capto L

2 - - - NA NA NA NA NA NA NA NA NA NA6 NA NA NA - - - - - - - - - -20* ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++50† + + + + + NA NA NA NA + + + +* The 20 μL plate is the preferred plate for the first set of experiments.† The 50 μL plate may be used for certain experiments, for example when protein concentrations are in the higher range or when there is a need for high amounts of sample for analysis.

Medium volumeper well (μL)

Butyl-SSepharose 6

Fast Flow

OctylSepharose 4

Fast Flow

ButylSepharose 4

Fast Flow

PhenylSepharose 6

Fast Flow (low sub) Capto Octyl Capto Butyl

PhenylSepharose 6

Fast Flow (high sub)Capto Phenyl

(high sub)

6 - - - - - - - -50 ++ ++ ++ ++ ++ ++ ++ ++

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Consistent well-to-well and plate-to-plate performance is obtained regardless of mode of operation and ensures high reproducibility of results. Figure 4 shows binding capacities determined in a 2 μL PreDictor Capto S plate after overloading it with protein. The relative standard deviation (RSD) calculated over several plates is 4.5%. This low variation in binding capacity clearly demonstrates the low variation in media volume between wells when compared to the variability of the UV assay (RSD = 3%).

High well-to-well and plate-to-plate reproducibility

Fig 4. Reproducibility of capacities for chymotrypsin (60 min incubation) determined in a 2 μL PreDictor Capto S plate. The solid red line corresponds to the average value, dashed lines denote ± 2 standard deviations. Columns 1 to 12 on the PreDictor plate are denoted by the alternating colored bars, indicating the absence of any significant edge effects.

0

50

100

150

200

Easy scale-up with reliable resultsThe optimized purification protocol can easily be scaled up as all chromatography media are available in prepacked columns and as bulk media. The correlation between data obtained with PreDictor plates and those obtained in column experiments is very good which makes the plates an excellent tool for initial screening of process conditions. Figure 5 shows the results from screening for optimal loading conditions for conalbumin on Capto S, with the same experiment performed both on PreDictor plates (Figure 5A) and in a packed column (Figure 5B).

Fig 5. Determination of loading conditions for conalbumin on Capto S. (A) Binding capacities at 60 min in PreDictor Capto S 2 μL plates. Error bars represent one standard deviation, based on triplicates. B) Dynamic binding capacities at 10% breakthrough. Residence time 2 min, column Tricorn™ 5/100 (CV 2 mL).

Fig 6. Overview of functionalities provided by Assist software forPreDictor plates.

Type of study

Experimental design

Plate data

Replicate plot

Plate layout

Experimental protocol

Mass balance

Adsorption isotherm

Response curve

Response surface

Lab instructions

Experimental setup Data management Data analysis

Conalbumin shows non-traditional behavior when binding to the cation exchanger (i.e., the highest binding capacity is obtained at intermediate ionic strength instead of at lowest ionic strength). The results clearly show that this non-traditional behavior is captured equally well in the plate as in the column experiment. For further details on non-traditional behavior, see Application Note, Screening and optimization of loading conditions on Capto S (28-4078-16).

In order to facilitate the high-throughput methodology, Assist software has been developed to support PreDictor plates.The software guides the user through the experimental workflow, assists in setting up the experiments, and manages and evaluates the data (Fig 6).

Assist software suggests an experimental design based on the type of study (e.g., binding, wash, elution, or determination of adsorption isotherms), and based on the experimental conditions (e.g., buffer system, pH, or salt concentration). It also documents the chosen experimental design.

Assist software for PreDictor plates

A) B)

Data evaluation includes calculation and visualization of mass balance, and calculation and visualization of response curves and surface plots. In this way, the effects of experimental conditions on responses such as binding capacity or yield can easily be identified. Table 4 summarizes the specifications for Assist software.

180

140

160

100

120

60

80

20

40

Bind

ing

capa

city

(mg/

mL)

00 0.05 0.1 0.15 0.2

Ionic strength (M)

pH 4.75

pH 5.25

pH 4.25

160

140

120

100

40

20

60

80

00 0.05 0.1 0.15 0.2

pH 4.75

pH 5.25

pH 4.25

Dyn

amic

bin

ding

cap

acity

(mg/

mL)

Ionic strength (M)

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Parallel screening in a miniaturized format allows for higher throughput, better process understanding, and low sample consumption. The availability of PreDictor 96-well filter plates, prefilled with GE Healthcare Life Sciences chromatography media, and Assist software for experimental setup and data evaluation enables high-throughput screening of downstream purification process conditions while keeping sample consumption at a minimum.

Summary

Table 4. Specifications for Assist software

Operating system Windows™ XP, Vista, or Windows 7 32-bitWorkstation Intel dual-core recommended

500 MB (1 GB recommended)Video card capable of 24-bit colorScreen resolution min. 1024 x 768 pixelsCD or DVD drive required.

E-licenses Required

Single medium plates Quantity Code No.PreDictor Q Sepharose Fast Flow, 50 µL 4 x 96-well filter plates 28-9432-71PreDictor SP Sepharose Fast Flow, 6 µL 4 x 96-well filter plates 28-9432-72PreDictor SP Sepharose Fast Flow, 20 μL 4 x 96-well filter plates 28-9432-73PreDictor SP Sepharose Fast Flow, 50 μL 4 x 96-well filter plates 28-9432-74PreDictor Butyl Sepharose 4 Fast Flow 6 µL 4 x 96-well filter plates 17-0980-16

PreDictor Butyl Sepharose 4 Fast Flow 50 µL 4 x 96-well filter plates 17-0980-17PreDictor Butyl-S Sepharose 6 Fast Flow 6 µL 4 x 96-well filter plates 17-0978-16PreDictor Butyl-S Sepharose 6 Fast Flow 50 µL 4 x 96-well filter plates 17-0978-17PreDictor Capto Butyl, 6 μL 4 x 96-well filter plates 17-5459-16PreDictor Capto Butyl, 50 µL 4 x 96-well filter plates 17-5459-17PreDictor Capto Octyl, 6 µL 4 x 96-well filter plates 17-5465-16PreDictor Capto Octyl, 50 µL 4 x 96-well filter plates 17-5465-17PreDictor Capto Phenyl (high sub), 6 µL 4 x 96-well filter plates 17-5451-16PreDictor Capto Phenyl (high sub), 50 µL 4 x 96-well filter plates 17-5451-17PreDictor Octyl Sepharose 4 Fast Flow, 6 µL 4 x 96-well filter plates 17-0946-16PreDictor Octyl Sepharose 4 Fast Flow, 50 µL 4 x 96-well filter plates 17-0946-17PreDictor Phenyl Sepharose 6 Fast Flow (high sub), 6 μL 4 x 96-well filter plates 17-0973-16PreDictor Phenyl Sepharose 6 Fast Flow (high sub), 50 μL 4 x 96-well filter plates 17-0973-17PreDictor Phenyl Sepharose 6 Fast Flow (low sub), 6 µL 4 x 96-well filter plates 17-0965-16PreDictor Phenyl Sepharose 6 Fast Flow (low sub), 50 µL 4 x 96-well filter plates 17-0965-17

Screening plates Quantity Code No.PreDictor AIEX Screening, 2 µL/6 µL 4 x 96-well filter plates 28-9432-88PreDictor AIEX Screening, 20 μL 4 x 96-well filter plates 28-9432-89PreDictor CIEX Screening, 2 µL/6 µL 4 x 96-well filter plates 28-9432-90PreDictor CIEX Screening, 20 μL 4 x 96-well filter plates 28-9432-91PreDictor HIC Screening High Hydrophobicity, 6 μL 4 x 96-well filter plates 28-9923-92PreDictor HIC Screening High Hydrophobicity, 50 μL 4 x 96-well filter plates 28-9923-97PreDictor HIC Screening Low Hydrophobicity, 6 μL 4 x 96-well filter plates 28-9923-95PreDictor HIC Screening Low Hydrophobicity, 50 μL 4 x 96-well filter plates 28-9923-98

Adsorption isotherm plates Quantity Code No.PreDictor Capto Q Isotherm* 4 x 96-well filter plates 28-9432-78PreDictor Capto S Isotherm* 4 x 96-well filter plates 28-9432-79PreDictor Capto DEAE Isotherm* 4 x 96-well filter plates 28-9432-80PreDictor Capto MMC Isotherm* 4 x 96-well filter plates 28-9432-81PreDictor Capto adhere Isotherm* 4 x 96-well filter plates 28-9432-82PreDictor MabSelect Isotherm* 4 x 96-well filter plates 28-9432-83PreDictor MabSelect SuRe Isotherm* 4 x 96-well filter plates 28-9432-84PreDictor MabSelect Xtra Isotherm* 4 x 96-well filter plates 28-9432-85PreDictor Q Sepharose Fast Flow Isotherm* 4 x 96-well filter plates 28-9432-86PreDictor SP Sepharose Fast Flow Isotherm* 4 x 96-well filter plates 28-9432-87

* Plates manufactured on request.

Ordering InformationProducts Quantity Code No.PreDictor Capto Q, 2 µL 4 x 96-well filter plates 28-9257-73PreDictor Capto Q, 20 µL 4 x 96-well filter plates 28-9258-06PreDictor Capto Q, 50 µL 4 x 96-well filter plates 28-9258-07PreDictor Capto S, 2 µL 4 x 96-well filter plates 28-9258-08PreDictor Capto S, 20 µL 4 x 96-well filter plates 28-9258-09PreDictor Capto S, 50 µL 4 x 96-well filter plates 28-9258-10PreDictor Capto DEAE, 2 µL 4 x 96-well filter plates 28-9258-11PreDictor Capto DEAE, 20 µL 4 x 96-well filter plates 28-9258-12PreDictor Capto DEAE, 50 µL 4 x 96-well filter plates 28-9258-13PreDictor Capto MMC, 6 µL 4 x 96-well filter plates 28-9258-14PreDictor Capto MMC, 20 µL 4 x 96-well filter plates 28-9258-15PreDictor Capto MMC, 50 µL 4 x 96-well filter plates 28-9258-16PreDictor Capto MMC ImpRes, 6 µL 4 × 96-well filter plates 17-3716-30PreDictor Capto MMC ImpRes 20 µL 4 × 96-well filter plates 17-3716-31PreDictor Capto adhere, 6 µL 4 x 96-well filter plates 28-9258-17PreDictor Capto adhere, 20 µL 4 x 96-well filter plates 28-9258-18PreDictor Capto adhere, 50 µL 4 x 96-well filter plates 28-9258-19PreDictor Capto adhere ImpRes, 6 μL 4 × 96-well filter plates 17-3715-30PreDictor Capto adhere ImpRes, 20 μL 4 × 96-well filter plates 17-3715-31PreDictor Capto SP ImpRes, 6 μL 4 x 96-well filter plates 17-5468-16PreDictor Capto SP ImpRes, 20 μL 4 x 96-well filter plates 17-5468-17PreDictor Capto Q ImpRes, 6 μL 4 x 96-well filter plates 17-5470-16PreDictor Capto Q ImpRes, 20 μL 4 x 96-well filter plates 17-5470-17PreDictor MabSelect, 6 µL 4 x 96-well filter plates 28-9258-20PreDictor MabSelect, 20 µL 4 x 96-well filter plates 28-9258-21PreDictor MabSelect, 50 µL 4 x 96-well filter plates 28-9258-22PreDictor MabSelect SuRe, 6 µL 4 x 96-well filter plates 28-9258-23PreDictor MabSelect SuRe, 20 µL 4 x 96-well filter plates 28-9258-24PreDictor MabSelect SuRe, 50 µL 4 x 96-well filter plates 28-9258-25PreDictor MabSelect SuRe LX, 6 μL 4 x 96-well filter plates 17-5474-30PreDictor MabSelect SuRe LX, 20 μL 4 x 96-well filter plates 17-5474-31PreDictor MabSelect SuRe LX, 50 μL 4 x 96-well filter plates 17-5474-32PreDictor MabSelect Xtra, 6 µL 4 x 96-well filter plates 28-9432-75PreDictor MabSelect Xtra, 20 μL 4 x 96-well filter plates 28-9432-76PreDictor MabSelect Xtra, 50 μL 4 x 96-well filter plates 28-9432-77PreDictor Capto L, 6 μL 4 x 96-well filter plates 17-5478-30PreDictor Capto L, 20 μL 4 x 96-well filter plates 17-5478-31PreDictor, Capto L, 50 μL 4 x 96-well filter plates 17-5478-32PreDictor Q Sepharose Fast Flow, 6 µL 4 x 96-well filter plates 28-9432-69PreDictor Q Sepharose Fast Flow, 20 μL 4 x 96-well filter plates 28-9432-70

28-9258-39 AF 03/2013

imagination at work

For local office contact information, visitwww.gelifesciences.com/contact

www.gelifesciences.com/predictor

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GE, imagination at work, and GE monogram are trademarks of General Electric Company.

ÄKTA, AxiChrom, BioProcess, Capto, HiScreen, HiTrap, MabSelect, MabSelect SuRe, MabSelect Xtra, PreDic-tor, ReadyToProcess, Sepharose, Tricorn, and UNICORN are trademarks of GE Healthcare companies.RoboColumn is a trademark of Atoll GmbH Limited. Windows and Intel are trademarks of Microsoft Corp.

Any use of UNICORN and Assist software is subject to GE Healthcare Standard Software End-User License Agreement for Life Sciences Software Products. A copy of this Standard Software End-User License Agree-ment is available on request.

© 2008-2013 General Electric Company — All rights reserved. First published Jan. 2008

All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.

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Software Code No.Assist 1.2 Software package 28-9969-17Assist 1.2 1-User License* 28-9453-97* Assist v1.2 also works with previous software versions 1.0 and 1.1.

Related ProductsAccessories Quantity Code No.Collection Plate, 500 µl V-bottom 5 × 96 well plates 28-4039-43Microplate Foil, 96-well 100 × self-adhesive,

transparent plastic foils

BR-1005-78

Prepacked columns Quantity Code No.HiScreen Capto Q 1 × 4.7 mL 28-9269-78HiScreen Capto S 1 × 4.7 mL 28-9269-79HiScreen Capto DEAE 1 × 4.7 mL 28-9269-82HiScreen Capto MMC 1 × 4.7 mL 28-9269-80HiScreen Capto adhere 1 × 4.7 mL 28-9269-81HiScreen Capto adhere ImpRes 1 × 4.7 mL 17-3715-20HiScreen Capto MMC ImpRes 1 × 4.7 mL 17-3716-20HiScreen Capto SP ImpRes 1 × 4.7 mL 17-5468-15HiScreen Capto Q ImpRes 1 × 4.7 mL 17-5470-15HiScreen MabSelect 1 × 4.7 mL 28-9269-73HiScreen MabSelect SuRe 1 × 4.7 mL 28-9269-77HiScreen MabSelect SuRe LX 1 × 4.7 mL 17-5474-15HiScreen MabSelect Xtra 1 × 4.7 mL 28-9269-76HiScreen Capto L 1 × 4.7 mL 17-5478-14HiScreen Butyl FF 1 × 4.7 mL 28-9269-84HiScreen Butyl-S FF 1 × 4.7 mL 28-9269-85HiScreen Octyl FF 1 × 4.7 mL 28-9269-86HiScreen Phenyl FF (high sub) 1 × 4.7 mL 28-9269-88HiScreen Phenyl FF (low sub) 1 × 4.7 mL 28-9269-89HiScreen Capto Phenyl (high sub) 1 × 4.7 mL 28-9924-72HiScreen Capto Butyl 1 × 4.7 mL 28-9924-73

Related literaturePredictor literature Code No.HandbooksHigh throughput process development with PreDictor plates 28-9403-58Application notesScreening of loading conditions on Capto S using a new high-throughput format, PreDictor plates

28-9258-40

High-throughput screening of elution pH for monoclonal antibodies on MabSelect SuRe using PreDictor plates

28-9277-92

Adsorption equilibrium isotherm studies using a high-throughput method

28-9403-62

High-throughput screening and optimization of a protein capture step in a monoclonal antibody process

28-9468-58

High-throughput screening and process development for capture of recombinant pro-insulin from E. coli

28-9966-22

High-throughput screening of HIC media in PreDictor plates for capture of recombinant Green FluorescentProtein from E. coli

28-9964-49

High-throughput process development for design of cleaning-in-place protocols

28-9845-64

Screening and optimization of loading conditions on Capto S 28-4078-16Mini PosterHigh-throughput screening of elution conditions on Capto MMC using PreDictor plates

28-9277-90

Additional literature Code No.Data filesPreDictor RoboColumn 28-9886-34Capto S, Capto Q, and Capto DEAE 11-0025-76Capto MMC 11-0035-45Capto adhere 28-9078-88Capto MMC ImpRes 29-0356-74Capto adhere ImpRes 29-0344-97Capto SP ImpRes and Capto Q ImpRes 28-9837-63MabSelect 18-1149-94MabSelect SuRe 11-0011-65MabSelect SuRe LX 28-9870-62MabSelect Xtra 11-0011-57Capto L 29-0100-08Sepharose Fast Flow ion exchangers 18-1020-66Capto Phenyl (high sub), Capto Butyl 28-9558-57Butyl-S Sepharose 6 Fast Flow 11-0026-34Phenyl Sepharose 6 Fast Flow (low sub), Phenyl Sepharose 6 Fast Flow (high sub)

18-1020-53

Butyl Sepharose 4 Fast Flow 18-1020-70