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  • D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\
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artsandscience.usask.ca/profile/JMcBeth Joyce M McBeth, Viorica (Ibi) F Bondici, Scott D Colville Geological Sciences, University of Saskatchewan, Saskatoon, SK Kris Bradshaw, Jay Grosskleg, and Trevor Carlson Federated Co-operatives Ltd., Saskatoon, SK Wenhui Xiong, Chris Mathies, Marshall Pachal, and Robert Bechard Stantec Consulting Ltd., Saskatoon, SK Microbial community response to biostimulation in a hydrocarbon contaminated site SEIMA SustainTech, 12 April 2016

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Page 1: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

artsandscience.usask.ca/profile/JMcBeth

Joyce M McBeth, Viorica (Ibi) F Bondici, Scott D ColvilleGeological Sciences, University of Saskatchewan, Saskatoon, SKKris Bradshaw, Jay Grosskleg, and Trevor Carlson Federated Co-operatives Ltd., Saskatoon, SKWenhui Xiong, Chris Mathies, Marshall Pachal, and Robert BechardStantec Consulting Ltd., Saskatoon, SK

Microbial community response to biostimulation in a hydrocarbon contaminated site

SEIMA SustainTech, 12 April 2016

Page 2: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Intro: what is in situ bioremediation and why use it? Site overview Hypotheses and objectives Research plan and approach Timeline and milestones

Talk Plan

Page 3: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

In situ bioremediation process where natural microbial communities at acontaminated site are bio-stimulated to degrade pollutants, such as petroleum hydrocarbons (PHCs) Why would we use in situ bioremediation?minimally invasive in comparison with other treatment methods (e.g. excavation, ex situ remediation)

Page 4: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

In situ bioremediation Challenges Site specific biogeochemical conditions Selection of amendments (type and concentration) Establish and maintain optimal conditions that favourhydrocarbon degradation

Page 5: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Field Site: 33rd Street and Avenue P North, Saskatoon, SK

View of convenience store and gas station, looking north• Gas station active from 1950s through present day• Leaky underground storage tanks from 1950s through 1980s• Contamination extends to the south of the station across the road and under an

apartment block

View of apartment block and field site during fall 2015 monitoring well drilling, facing west

Page 6: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Field Site: 33rd Street and Avenue P North, Saskatoon, SK

• Major effort by FCL over the past 3 years to remove PHC from the site: physical removal using multiphase extraction and bioventing. • Some remaining contamination that is recalcitrant to removal.• Next step: stimulate indigenous microbes to attempt to speed up natural biodegradation of the PHCs

Page 7: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

HypothesesBiostimulation will speed up degradation of PHCs at the field siteBiostimulation will lead to measurable changes in the microbial community, groundwater quality, and mineralogy at the field site

Page 8: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

ObjectivesTo characterize microbiological, mineralogical, and geochemical conditions in site groundwater and soils:

Before biostimulation After biostimulation

To develop a biogeochemical model for in situ bioremediation of this field siteTo contribute to improved approaches for remediation of PHC sites in Western Canada (SIRCA initiative)

Page 9: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Research approach and timeline Sample pre-biostimulation conditions (Fall 2015, Spring 2016) Groundwater sampling:

a) Existing infrastructure (7 monitoring wells)b) Additional monitoring wells (6 wells drilled Fall 2015) – also took soil/sediment samples at this time

Biostimulation phase 1 (NSERC Engage, March – Sept 2016): a) add biostimulation amendments at field site (nitrogen and phosphorus sources), sample groundwater at monthly intervalsb) Selection of amendments informed by previous work by SIRCA members (Peak, Siciliano, FCL)

Biostimulation phase 2 (Summer, Fall 2016):a) Lab-based microcosm studies to test the efficacy of surfactant additions prior to field testingb) Field testing surfactant influence on biostimulation

Page 10: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Initial field sampling and drilling, Fall 2015Soil sediment cores Groundwater sampling

Page 11: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Methods

Field study:Initial conditions and post biostimulation amendments

Microbial diversity

Groundwater microbiology:Filter

Extract genomic DNAHigh throughput amplicon sequencing

Sequence data processingqPCR

Soil sediment microbiology:Extract genomic DNA

High throughput amplicon sequencingSequence data processing

Soil mineralogy and physical characterization

PXRD: mineralogy STXM: relationship of PHCs to clays

BMIT: CT of pore spacesGroundwater geochemistry

Geochemical parameters: BTEX, PHC F1/F2, TOC, COD, Fe, Mn, N, NH3, P, Sulfide, routine H2O analysesMicrocosm studies:Testing efficacy of different surfactants

tracking geochemical parameters (PHC, Fe, etc)

Page 12: D ] } ] o } u u µ v ] Ç } v } ] } ] u µ o ] } v - SEIMA 2016... · d z } )lhog vwxg\ ,qlwldo frqglwlrqv dqg srvw elrvwlpxodwlrqdphqgphqwv 0lfureldo glyhuvlw\ *urxqgzdwhu plfurelrorj\

Thank you!

artsandscience.usask.ca/profile/JMcBeth

McBeth NSERC Discovery Grant, Engage GrantFederated Cooperatives LtdCanadian Light Source (beamtime)StantecThis project is part of the Sustainable In Situ Remediation Cooperative Alliance (SIRCA)


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