cytospin 3 (74010101 iss. 02) - udh.med.sa · the service manual for the cytospin 3 bench top...

CYTOSPIN 3

74010101 Issue No 02

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CELL PREPARATION SYSTEM

SlWNDONI(>I”

Shandon Scientific Limited is an IS0 9001 Approved Company.

SERVICE MANUAL

I information contained in this document is intended for use by qualified personnel only. The calibration, scheduled servicing and the removal and replacement of parts should only be undertaken by personnel who have been formally trained in the appropriate servicing techniques and procedures.

I

LIFE SCIENCES INTERNATIONAL (EUROPE) LTD

SERVICE IidANUAL RECEIPT SKEET

When you receive this Cytospin 3 Service Manual, please complete the following:

Company/Agent Name: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Address: .....................................................................................................

........................................................................ ........................................... . .

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Signature: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Please return this completed Receipt Sheet to the Service Department at Life Sciences international (Europe) Ltd by fax (0928 565845) or by letter post to:

Life Sciences international (Europe) Ltd International Service Department Chadwick Road, Astmoor Runcorn, Cheshire, WA7 IPR England

These sheets will be used to complete our records and ensure you receive any amendments as and when they become available.

International Service Manager

SELANDONCYTOSPIN 3

MICROPROCESSOR CONTROLLED CENTRIFUGE

SERVICE: HANUAL

This manual has been designed as an aid to efficient servicing and fault diagnosis on the Cytospin 3 centrifuge machine.

In order to get the best results from the Cytospin 3 please consult the operators manual.

The service manual for the Cytospin 3 bench top centrifuge is designed to provide the locally based engineer with all the information he needs to install and maintain the Cytospin in good running order. It is essential that the maintenance routines are followed so that the operator gets the most out of the Cytospin.

In the event of a problem arising, the section on Fault Diagnosis should be referred to. This section contains information designed to help the service engineer locate and repair simple faults. For a more detailed description of the operation of the Cytospin 3, the Principles of Operation section should be referred to.

If you think you have identified and resolved the cause of a fault, remember to carry out any calibration of the part you have repaired or replaced, if necessary. Before handing over the Cytospin to the operator, make sure you have checked it out thoroughly using the serviceability test.

Finally, the manual provides you with a comprehensive list of any parts you may need to order.

Whilst every care has been taken to ensure that the information contained in this manual is correct, no liability can be accepted by Shandon Scientific Limited for any loss, damage or injury caused by any errors in, or omissions from, the information given.

All rights reserved. No part of this manual may be reproduced or recorded, including photo-copying, without the express written permission of Shandon Scientific Limited.

ii

AMENDMENTS

Each of these manuals is numbered and listed against a manual holder. Any further copies required for the service area you are responsible for will be listed under the same holder with a different sequential number.

As changes in the machine design necessitate changes in the manual, amendments will be sent to you to update the text: These amendments will involve adding and/or changing pages, the amendment log will help ensure all are up-to-date.

These manuals can only be obtained through Runcorn International Service Office.

AMENDMENT LOG

Each time an amendment is issued, fill in the number and date.

Amendment Number Date Signature

740-1-g 1 24.4.91 J J Curran

i i i

INDEX

ChaDter Title Page No

INSTALLATION 1

PROGRAMMING THE CYTOSPIN 3 2

SERVICEABILITY TEST 8

DAILY AND WEEKLY MAINTENANCE 11

SAFETY 14

STATIC DAMAGE TO MOS DEVICES 14

FAULT DIAGNOSIS AND REPAIR 15

FITTING, TESTING AND CALIBRATION 29

Motor 29 Lid 30 Drip tray 31 Damper adjustment 31 Guard barrier 32 Lid catch 32 Out of balance switch 33 Power supply 33 Heatsink 34 Nosecone 34

(a) Main PCB (b) Proximity switch (c) Front panel

34 34 35

9

10

11

APPENDIX

EXPLODED DIAGRAMS AND PARTS LIST 36

LIST OF DRAWINGS 48

CIRCUIT DIAGRAMS 49

PRINCIPLES OF OPERATION 51

(1) (2) (3) (4) (5) (6) (7) (8) (9)

(10)

Overview of System General Power Supply Microprocessor System Display and Keyboard Circuits Motor Drive Circuit Solenoid and Relay Drive Circuits Speed Watchdog and Out of Balance m, m, Hardware Watchdog and Open Collector Logic Motor, Opto Disc, Lid Lock and Balance-Switch

51 51 51 52 52 53 53 54

55

55

iv

1 INSTALLATION

1 Unpack the unit and carefully untie the mains lead (electrical cord).

2 Check the instrument for signs of damage in shipment (broken glass, broken plastic, dents, etc). If something is missing or damaged notify Shandon or your supplier immediately. Quote the order number(s), inspection number, serial number, date and number of the invoice.

3 Where necessary, fit a suitable plug to the mains lead (electrical cord). Make sure that the earth wire is connected and that the mains supply has an earth (ground) connection.

Instruments supplied to France, Germany and North America are fitted with plugs during manufacture. Other customers should connect the mains lead to a suitable rated fused plug as follows :

Brown wire - Live (L or L2) terminal Blue wire - Neutral (N or Ll) terminal Green/Yellow wire - Earth (E or Ground) terminal

Make sure that the voltage rating on the specification plate at the rear of the Cytospin and on thezinspection tag are the same as the mains supply rating.

Switch the instrument on by pressing the power switch.

4 Press the ‘Open Lid I button and open the safety cover. In the event of a power failure, the safety cover cannot be opened using the ‘Open Lid’ button. The small white cap on the left-hand side of the instrument should be removed. A pencil or rod pushed into the hole and pressed firmly will open the safety cover. Remove the sealed head and set it on the bench beside the instrument. Remove all internal packing.

5 Unpack the basic kit and check against the items list in the Operator Manual.

2 PROGRAHHING THE CYTOSPIN 3

This section illustrates how the Cytospin 3 is programmed and how programs may be stored, viewed and run. Please go through each example completely to gain a full understanding of how to effectively use your unit. Ensure that the Cytospin lid is closed before running this unit.

N

(a) Entering and Running a Program

Speed required = 1500 rpm, Time required = 5 minutes, Acceleration rate = low.

Speed Time Accel Audible Operation Display Display Display Signal

1 Switch 'ON' 2 Press 'SET TIME' 3 Press ‘5' 4 Press 'ENTER' 5 Press 'SET SPEED' 6 Press ‘1' 7 Press ‘5' 8 Press ‘0' 9 Press ‘0' 10 Press 'ENTER' 11 Press 'LOW'

0 0 0 0 0 1 15 150 1500 1500 1500

0 0 5 5 5 5 5 5 5 5 5

HIGH 3 Set Audible HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone LOW valid key tone

The Cytospin 3 is now programmed. This program may now be initiated, ie run (Operation step 12) and/or it may be stored in memory with a designated program number (see (b) below).

12 Press 'START' 0 5 LOW valid key tone Lid Locked 13 Acceleration Period O-1500 5 LOW Lid Locked 14 Once Speed Attained 1500 5-o LOW Lid Locked 15 Deceleration Period 1500-O LOW Lid Locked 16 Head Stops Rotating 0 : LOW 3 Set Audible Lid Unlocked 17 After 3 Set Audible Signal 1500 5 LOW Lid Unlocked

(b) Storing (Saving) a Program in Memory

W

Program Number 1 designated

Operation

1 Press ‘SAVE’ 2 Press Program ‘1’ 3 Press ‘ENTER’

Program Speed Display Display

0 1500 1 1500 1 1500

Time Display

5 5 5

Accel Display

LOW LOW LOW

Program Number 1 is now entered into the memory. This may be done either before or after.operation Step 12 above. (Not during running of the Cytospin).

(c) Running a Program From Memory

Program Number 1 Program Speed Time Accel

Operation Display Display Display Display

1 Press ‘LOAD’ 0 - previous program entered - 2 Press Program ‘1’ 1 1500 5 LOW 3 Press ‘ENTER’ 1 1500 5 LOW 4 Press ‘START’ 1 0 5 LOW

Program Number 1 has now been recalled from memory and initiated or run.

(d) Viewing Previously Entered Program(s) ‘.

The operator having stored several programs in memory (up to 9) may wish to later view these previously entered programs to inspect speed/time/accel parameters to act as a reminder in selecting the desired one.

Let us assume that Section 2 (a) and (b) have been followed entering 9 different programs in memory, and that the desired parameters are speed: 800 rpm, time: 8 minutes, accel: med, but the actual program number cannot be immediately remembered by the operator.

Program Speed Time Accel Operation Display Display Display Display

1 Press ‘LOAD’ 0 0 0 HIGH 2 Press Program ‘1’ 1 1500 5 LOW 3 Press Program $2’ 2 800 8 MED

As you can see in this example, having sequenced through the above steps, Program Number 2 contains the desired operational parameters (speed/time/accel) and can now, if desired, be run. Simply follow operational section 2 (c) 3 through 4.

GUIDANCE NOTES

1 To Repeat a Particular Program

The operator has the choice of either,

(a> storing a program in memory for future use and subsequently running this program from memory, as detailed in section 2 (b) and (c) or

(b) simply closing the lid following the previous run (with new samples correctly loaded) and pressing ‘START’ - as detailed in Section 2 (a) steps 12 - 17 inclusive.

2 To Alter a Program Mode Parameter (by using the ‘CANCEL’ button)

(a) Press ‘CANCEL’ after ‘SET TIME’ or ‘SET SPEED’ to cancel time or speed previously entered into memory. Please note it is essential to then select alternative speed/time parameter(s).

(b) Alternatively pressing ‘CANCEL’ systematically after having selected the ‘ENTER’ button for a time/speed parameter, will revert both the speed and time displays to zero.

Pressing the ‘CANCEL’ button again will cause the speed/time/accel displays to revert back to the previous program parameters entered.

3 If Program Number 1 is required but the Lid is not closed correctly:

Speed Time Accel Audible Operation Display Display Display Signal

1 Press 'START' 1500 5 LOW -

2 Close Lid 1500 5 LOW -

3 Press 'START' 0 5 LOW -

From this point follow steps 12 - 17 in Section 2 (a).

Lid Alarm Lock LED Display

Unlocked Lid Lock & Open Illuminated Unlocked Lid Lock No Longer

Illuminated Locked Lid Lock No Longer

Illuminated

4 If Program Number 1 is required but the centrifuge head has been loaded incorrectly and is out of balance

Operation Speed Time Accel Audible Lid Alarm Display Display Display Signal Lock LED Display

1 2 3

Close lid 1500 Press 'START' 0 Acceleration Period 'OUT OF 0 BALANCE' detected Deceleration Period O-1500

5 5 5

LOW Unlocked LOW Locked LOW Alarm Locked

4 5 LOW Alarm Locked

5 Head Stops Rotating o-1500* 0 LOW Alarm Locked

6 Switch 'OFF' 7 Switch ‘ONI 0 8 Re-select Program 1 1500 9 Correct 'OUT OF BALANCE' 1500 10 Press 'START' 0 11 Follow Section 2 (a) step 12 on.

LOW 3 set Unlocked LOW Unlocked LOW Unlocked LOW Unlocked

Balance Illuminated Balance 'Illuminated Balance Illuminated

5 Invalid Soeeds Lower Than 200 mm

The Cytospin 3 sample chamber assembly will not 200 rpm is achieved.

'TILT' into the upright position unless a speed of The program memory will reject as INVALID any speed lower than 200 rpmr as

illustrated in this example when 190 rpm is entered.

Operation Speed Time Accel Audible Display Display Display Signal

1 Press 'SET TIME' 2 Press ‘5' 3 Press 'ENTER' 4 Press 'SET SPEED' 5 Press '1' 6 Press '9' 7 Press ‘0' 8 Press 'ENTER'

0 0 0 0 1 19 190 0

HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH valid key tone HIGH invalid speed

Action to be taken i select and enter a value of 200 rpm or higher.

6 Invalid Speeds Higher Than 2000 rpm

The Cytospin 3 program memory will reject any speed entered greater than 2000 rpm. As an illustration, examine the following procedures when 2010 rpm is programmed.

Operation Speed Time Accel Audible Display Display Display Signal

1 As Number 5 above Steps l-4 0 5 HIGH 2 Press ‘2'

valid key tone 2 5 HIGH

3 Press ‘0' valid key tone

20 5 HIGH valid key tone 4 Press ‘1' 201 5 HIGH valid key tone 5 Press ‘0' 2010 5 HIGH 6

valid key tone Press 'ENTER' 0 5 HIGH invalid speed

Action to be taken - select and enter a value of 2000 rpm or lower.

7 Invalid Speed/Time Parameters

The Cytospin 3 will not start without a valid speed and time entered and displayed. Similarly a program cannot be saved if the speed and time are not valid.

8 ‘!’ Light (Overspeed Indication/Rotor Jam)

The ‘! ’ light is illuminated in the event that speed control becomes irregular during a run, due to electrical interference or instrument malfunction.

Similarly should the sealed head assembly fail to start rotating due to mechanical or electrical malfunction, then the ‘!I light will be illuminated after approximately 3 seconds. An audible alarm signal will sound, and the ‘drive’ to the sealed head motor will be disconnected.

Turn the power off, and then refer to Section 2, No 4, Steps 7, 8, 10, 11.

SWVICEABILITYTEST

Introduction

This procedure describes the test which can be carried out, at any time, on the Cytospin 3, to ascertain if the machine is functioning correctly. In the following tests a sealed head is fitted without cell or cell holders.

Instruments Required

One Multimeter, one 1OOOV Megger, one Stopwatch.

Insulation Tests

Before applying power to the machine the following insulation tests must be carried out. Using the multimeter, check the resistance path between the earth wire and any metal parts of the machine, this resistance is to be less than ORl. Connect the 1OOOV megger between the live and neutral wires on the mains power lead. With the power switch in the off position, the resistance should be greater than 100 megohms. Short the live and neutral wires of the mains power lead together. With the power switch in the ON position the resistance between the live/neutral wires and the earth should be greater than 25 megohms. If above is correct, apply power to machine.

Procedure

When power is applied to the Cytospin 3 thejdisplay panel should initialise to the following (if not, consult Fault Diagnosis Section and/or Principles of Operation) and the alarm should sound for three seconds.

Program Display Speed Display Time Display HIGH MED LOW STOP START BAL ! LID LOCK OPEN LID

0 0 0 ON OFF OFF ON OFF OFF OFF OFF OFF

Keyboard Tests

Press SET SPEED, check speed 0 flashes.

Press 0 - 9 in turn and check that digit appears on the right hand side of the speed display and "rolls round".

Press CANCEL, check that display reads 0 flashing.

Press CANCEL, check that display reads 0.

8

Press SET TIME, check that display reads 0 flashing.

Press 0 - 9 in turn and check that digit appears on right-hand side of time display and "rolls round".

Press CANCEL, check that display reads 0 flashing.

Press CANCEL, check that display reads 0.

Press LOAD, check program 0 flashes.

Press 0 - 9 in turn and check that the digit appears in the program window.

Press CANCEL, check program 0 flashes.

Press CANCEL, check program displays 0.

Operation Tests

Press SET SPEED.

Press 2000.

Press ENTER, display should read 2000.

Press SET TIME.

Press 10.

Press ENTER, display should read 10.

Press START.

The Start LED should now come on and the Stop LED go off. The sealed head will begin to rotate and accelerate quickly until the SET SPEED is reached. The speed display will now read the actuaZ speed of the sealed head in rpm and time display will begin to count down towards 0.

When the time display reaches 0 the sealed head will come to a halt. The Start LED will go off and the Stop LED will come on. The speed display will now read the SET SPEED (ie 2000) and the time display will reset from 0 to 10. The audible alarm will sound at the end of operation.

Press SET SPEED.

Press 200.

Press ENTER, display will now read 200.

Remove the cells and cell holders from the sealed head, replace the sealed head in the machine and close the lid.

Press SET TIME.

Press 30.

Press ENTER, the display should read 30.

Press HIGH ACCEL, the HIGH ACCEL LED should come on and the LOW ACCEL LED should go off.

Press START, the sealed head should begin to rotate.

Using the stopwatch, check that the timer counts down in 1 minute intervals. Check that the speed display remains at 2000 rpm and that the head remains quiet throughout the 30 minute program.

When the timer reaches 0 the machine should stop and the audible alarm sound. The time display should reset to 30.

If the Cytospin 3 passes the above functional tests it can be considered to be serviceable. If the machine fails to pass any of the tests then reference must be made to the appropriate part of the servicing manual.

10

DAILY AND WEEKLY MAINTENANCE

CLEANING AND STERILISING THE CYTOSPIN 3

All the components of the Cytospin 3 which are likely to become contaminated have been designed to be easily cleaned using mild detergent solutions, and then sterilised using a wide variety of agents.

In the interest of laboratory safety, Shandon recommends that suggested sterilisation procedures are adhered to.

All the components of the sealed head, the centrifuge bowl liner, safety cover and front panel, are designed to be easily cleaned.

Each component part is dealt with individually and if the instructions are followed, no damage will occur. This is particularly important , since any chemical attack on the components of the sealed head or hinged safety cover will weaken <;-iz -p:?rial and create a serious hazard.

When handling, cleaning or sterilising any component, it is advisable to wear gloves as they offer protection against infection and chemical effects on the skin due to the detergent and/or disinfectants being used. After cleaning and sterilising any component, it should be examined for damage. If in doubt as to its state, it should not be used on the centrifuge until inspected by your Safety Officer.

IMPORTANT NOTE

If you are in doubt as to the type of disinfectant you are using, many brands do not declare the nature of the active ingredients , check with the manufacturer before using the solution with any component part of the Cytospin 3.

HYPOCHLORITE based disinfectants should NEVER be used to sterilise centrifuges of any type.

FRONT PANEL

Frequency:

Clean :

Sterilise:

Notes: a)

b)

Weekly and after any spillage.

Use a damp cloth to clean panel; do not use abrasive powders, these will damage the surface of the panel.

Use 2% glutaraldehyde prepared according to manufacturer’s instructions.

Never clean the front panel with Xylene, Toluene or similar solvents.

The front panel can be polished if required with a liquid or spray furniture polish.

11

HINGED SAFETY COVER

Frequency: Daily and after any serious spillage.

Clean: Use warm soapy water, DO NOT use abrasive powders, these will scratch the surface and make it more difficult to clean and also create a potential source of cross- contamination.

Sterilise: Use 2% glutaraldehyde prepared according to manufacturer's instructions.

Note: DO NOT clean the hinged cover with any solvents - these could weaken the safety cover.

BOWL LINER

Frequency: Daily and after any serious spillage.

Clean: Use warm soapy water, do not use abrasive powders, they will create scratches which may harbour micro-organiss and make sterilisation difficult.

Sterilise: Use 2% glutaraldehyde prepared according to manufacturer's instructions.

SEALED HEAD ASSEMBLY

Frequency: Daily and immediately after any-serious spillage.

Sterilise: The head assembly can be sterilised as a whole unit b autoclaving at 121OC (250'F) for 15 minutes. It is necessary to unlock the lid so that the pressure ins&&z the head will be equalised with those in the autoclave and to allow the free entry of steam into the interior of the head.

Clean: After sterilisation of the whole head, all components should be removed, washed in warm soapy water and dri& in an oven. (Maximum temperature 65'C or 149'F) befon re-assembly.

SEALED HEAD BASE

Frequency: Daily and immediately after any serious spillage.

Sterilise: Autoclave at 121OC (250OF) for 15 minutes. Wipe with2% glutaraldehyde or a phenolic disinfectant prepared according to manufacturer's instructions.

Clean:_ After sterilisation clean using warm soapy water, riiae in clean water and dry. DO NOT USE hard brushes to clean this component.

12

SEALED HEAD SLIDE CLIP ASSEMBLY SUPPORT PLATE

Frequency: Daily.

Sterilise: As Sealed Head - Base.

Clean: As Sealed Head - Base.

Note: This component is easily removed from the Sealed Head Base by removing two thumb screws. This enables the under surface of the support plate to be cleaned easily and also facilitates cleaning of the inside surface of the sealed head base. The support plate can be totally immersed in 2% glutaraldehyde disinfectant if required-

SEALED HEAD LID

Frequency: Daily.

Sterilise: As Sealed Head - Base.

Clean: As Sealed Head - Base.

Note: The silicone rubber seal around the rim of the lid cau be easily removed and replaced. It is advisable to remove this seal at weekly intervals to enable all the surface of the lid to be cleaned. A new seal should Be fitted at intervals of 12 months.

CYTOCLIP

Frequency: Daily.

Sterilise: Autoclave at 121OC (250'F) for 15 minutes or immerse in 2% glutaraldehyde solution according to manufacturer's instructions.

Clean: Rinse in clean water before use and hot air dry.

Note: It is advisable to ensure that the pressure spring is unlocked, releasing any pressure from the-spring durQg sterilisation. It is also important to ensure that ube face of the slide clip is clean as any raised particles could cause microscope slides to break under the appl&ed 'gr force.

SAMPLE CHAMBER

Frequency: After use.

13

5 SAFETY

1) There are potentially lethal voltages in the Cytospin 3. Take all necessary precautions when working on live machines.

2) Beware of mechanically moving parts. They may move unexpec- tedly under the control of the microcomputer system.

6 STATIC DAHAGE TO HOS DEVICES

Some of the components used on the Cytospin 3 electronics are MOS devices and are sensitive to static damage.

1) Take all necessary precautions when handling.

2) Treat all unknown devices as MOS.

3 Remember, statically damaged devices may take weeks or months to fail.

DO NOT TAKE CHANCES

1.4

7 FAULT DIAGNOSIS AND REPAIR

If the Cytospin 3 stops working it will be because either a fault condition has occurred or a safety circuit has operated. Refer to the list of fault conditions below until you find the symptoms of your fault. Turn to the relevant page and work through the possible causes, methods of diagnosis and solutions. To retest the Cytospin after each repair or replacement, follow the serviceability test.

If the symptom of your fault is not listed below refer to principles of operation and the relevant circuit diagram.

Fault Condition

1

2

3

9

10

Front Panel LED's not illuminated

SPEED, TIME, PROG, ACCEL and STOP display LED's will not initialise on 'Power On'.

Page

16

18

STOP, START, HIGH and LOW ACCEL LED's will not initialise on 'Power On'.

19

Cytospin 3 will not accept program commands.

Lid lock LED lights, when Start button pressed.

Motor will not run.

Balance LED lights.

Balance LED fails to light (with out-of-balance sealed head).

20

21

23

24

26

! LED lights and motor stops (failure of motor speed control loop).

27

Lid solenoid not working. 28

the

THERE ARE POTENTIALLY LETHAL VOLTAGES IN THE MACHINE SO CARE MUST BE TAKEN WHEN TESTING OR MAKING ADJUSTMENTS ON LIVE MACHINES.

15

FAULT CONDITION 1: FRONT PANEL LED’S NOT ILLUMINATED

Possible Cause Diagnosis Solution

1.1 Mains supply has failed. Check mains supply with suitable Wait for supply to return or find meter or by examining another an alternative power source. appliance. Retest.

1.2 Incorrect mains power

supply l

Check that supply for 240V Cytospin is between 220V and 25OV, for 1lOV machine between 1OOV and 120V.

If not, connect to correct supply and retest using serviceability test.

1.3 Plug and fuse FSl not intact.

Check plug. Check that fuse FSl is in place and intact (Drawing E740-l-l), 0.8 amp for 24OV, 2 amp for 1lOV.

If not, fix properly or replace. Retest. If fuse blows again, investigate short circuits, earth (ground) leakage etc.

1.4 Faulty cable, switch or (a) Check there is mains input If not, inspect the cable and wiring. voltage at the input terminals wiring. Replace as necessary.

of SW1 (Drawing E740-l-l). Retest.

(b) Ensure SW1 is closed and check If not, replace SWl. Retest. if mains input voltage is present at output side of SW1 (Drawing E740-l-l).

FAULT CONDITION 1: FRONT PANEL LED'S NOT ILLUMINATED


1.5 Power supply from transformer plate.

Check at socket for the following nominal voltages (Drawing E740-l-l)

(a) AC Voltages (a) If not, test transformer Between pins 1 and 2: 24V AC windings for continuity (Drawing Between pins 5 and 6: 15V AC E740-l-1).

8V AC Typical resistances

Between pins 10 and 12: for windings are: Between pins 11 and 12: 8V AC Primary 16.0 R

Secondary 24V 0.4 R Secondary 15V 1.4 R Secondary 8-0-8V 0.5 9

(b) DC Voltages Between pins 7 and 8: 24V DC Between pins 9 and 8: 24V DC

(b) If not, check across bridge rectifier Bl for 24V DC and 24V AC (Drawing E740-l-l).

If correct, inspect and test associated plugs and'wiring for open or short circuits, earth (ground) leakage etc (Drawing E740-1-1). Replace as necessary. Retest.

FAULT CONDITION 2: SPEED, TIME, PROG, ACCEL AND STOP DISPLAY LED'S WILL NOT INITIALISE ON 'POWER ON'


2.1 Incorrect power supplies Check that 12V, 5V, -5V and OV DC If not, check through relevant to mains PCB. rails are available on Main PCB. supply circuit and replace as

necessary (Drawing F740-1-2, E740-l-l). Retest.

2.2 Mains PCB faulty. Check main PCB by replacing with serviceable PCB. Before replacing PCB check that the correct voltages are available. (Drawing E740-l-l) (Refer to voltage table, Principles of Operation).

If correct, leave new PCB in place.

FAULT CONDITION 3: STOP, START, HIGH AND LOW ACCEL LED’S WILL NOT INITIALISE ON ‘POWER ON’


3.1 Fault on main PCB. Check main PCB power supplies (voltage table, Principles of Operation). If correct, replace main PCB with serviceable main PCB (after checking voltages).


3.2 LED’s not functioning. Press the Act switch and check If correct, test the LED’s which that the Lo Act LED lights. are not functioning. Replace as Program the Cytospin and press necessary. Retest. the Start switch, check that the start LEd lights and that the Cytospin operates correctly.

3.3 Wiring or connection fault.

Check associated wiring and connectors for continuity, open or short circuits, earth (ground) leakages (Drawing F740-1-2, E740-l-l).

If not, replace as necessary. Retest.

FAULT CONDITION 4: CYTOSPIN 3 WILL NOT ACCEPT PROGRAM COMMANDS


4.1 Main PCB. Replace PCB with serviceable PCB. Before replacing PCB check that supply voltages are correct (voltage table, Principles of Operation).

If now correct, leave new PCB in place.

4.2 Fault on Cytospin 3 Check keyboard by fitting front If.not, replace as necessary. keyboard (front panel). panel to serviceable Cytospin. Retest.

4.3 Fault on molex connections between main PCB and keyboard

Check connectors and wiring. Replace as necessary. Retest.

FAULT CONDITION 5: LID LOCK LED LIGHTS WHEN START BUTTON PRESSED


5.1 Lid not closed properly. Check lid fully closed. If correct, retest.

5.2 Lid lock switch faulty.

(SW5) Check operation of SW5 as follows: If not, replace SW5 as necessary. With the lid fully closed, connect Retest. a continuity tester between pins 11 and 12 of socket 3 (Drawing E740-1-1) and check for continuity. Manually operate the solenoid (Sol 1) until the lid catch opens and the lid raises slightly (you should be able to hear the catch opening). There should now be an open circuit between pin 11 and 12 of socket 3.

5.3 Magnetic proximity switch Check the operation of SW3 as (SW3) faulty. follows:

Place a magnet on the top trim in such a position that it will close the proximity switch. Connect a continuity tester between pin 7 on PL2B and pin 11, PL4A (Drawing E740-1-1) continuity. Remove magnet and check that there is now an open circuit between these pins.

If not, replace SW3 as necessary. Retest.

If correct, operate Cytospin with external magnet in position. If machine now functions correctly (ie defect not apparent) this indicates that lid magnet is not closing proximity switch.

lo N

FAULT CONDITION 5: LID LOCK LED LIGHTS WHEN START BUTTON PRESSED


5.4 Fault on lid lock circuit Check associated wiring wiring. (Drawing E740-l-l).

Replace as necessary.

5.5 Main PCB fault. Replace main PCB with serviceable PCB. Before replacing PCB check that correct voltages are


available (voltage table, Principles of Operation).

FAULT CONDITION 6: MOTOR WILL NOT RUN


6.1 Motor fault. Check coil of motor for continuity (Drawing E740-l-l).

If not, replace motor as necessary. Retest.

6.2 Incorrect power supplies Check power supply circuits and If not, replace as necessary. to motor. components (Drawing E740-1-1). Retest.

6.3 Fault on Relay A. Check Relay A for correct operation (Drawing E740-1-1).

If not? replace as necessary. Retest.

6.4 Transistor (TRl) fault. Check transistor TRl for correct If not, replace as necessary. operation (Drawing E740-l-l). Retest.

6.5 Main PCB Replace main PCB with serviceable PCB . Before replacing PCB check that correct voltages are available (voltage table, Principles of Operation).


FAULT CONDITION 7: BALANCE LED LIGHTS

Possible Cause Diagnosis solution

7.1 Sealed head loaded incorrectly.

Check that sealed head is loaded correctly.

If correct, retest.

7.2 Vibration switch (SW4) Adjust vibration switch (refer to If correct, retest. incorrectly adjusted. adjustment procedure Testing and

Calibration section).

7.3 Vibration switch (SW4) Check operation of SW4 as follows: If not, replace SW4 as necessary. faulty. Using a continuity tester, test for Retest.

open circuit between pins 10 and 12, socket 3 (Drawing E740-1-1). With tester still connected physically, tilt the Cytospin to the side in order to close SW4. Viewing the Cytospin from the front, SW4 is positioned laterally at the rear of the machine.

FAULT CONDITION 7: BALANCE LED LIGHTS


7.4 Main PCB Fault Check main PCB by replacing with If correct, leave new PCB in place. serviceable PCB. Before replacing PCB check that correct voltages are available (voltage table, Principles of Operation).

FAULT CONDITION 8: BALANCE LED FAILS TO LIGHT (with sealed head out of balance)


8.1 Vibration switch (SW4) Adjust vibration switch (refer to If correct, retest. incorrectly adjusted. adjustment procedure Testing and

Calibration section).

8.2 Vibration switch (SW4) or Check switch and wiring (refer to associated wiring faulty. Drawing E740-l-l and Fault

Diagnosis, paragraph 8.3).


8.3 Faulty LED. Test LED using diode tester (Drawing E740-1-2).


8.4 Main PCB fault. Check main PCB by replacing PCB with serviceable PCB. Before replacing PCB check that correct voltages are available (voltage table, Principles of Operation).


N -4

FAULT CONDITION 9: ! LED LIGHTS AND MOTOR STOPS (failure of motor speed control loop)


9.1 Fault on control opto sys tern.

With ‘Power On’ (but start switch not pressed) connect voltmeter between pins 1 and 2 on sensor PCB plug (Drawing E74@-1-1). Slowly turn the sealed head assembly (or drive boss) by hand and check that the output from the opto is present.

If not, check opto transistor, disc encoder, wiring etc. Replace as necessary. Retest.

9.2 Main PCB fault. Check main PCB by replacing with serviceable PCB. Before replacing PCB check that correct voltages are available (voltage table, Principles of Operation).


FAULT CONDITION 10: LID SOLENOID WILL NOT OPERATE


10.1 Fault on transistor T12 With solenoid button pressed check (a) If correct, check solenoid and and T13 (main PCB). output of T12 and pin 1 PL2B main PCB catch assembly. Replace as

(Drawing F740-1-2). necessary. Retest.

(b) If not, check transistor T12 and T13, microswitch and wiring. Replace as necessary. Retest.

8 FIT'UXG, TESTING AND CBLIBRATION

All internal screws and nuts to be fixed with Loctite Screwlok, WITH THE EXCEPTION OF ANY IN CONTACT WITH PLASTICS, where Araldite (eg lid) must be used.

(1) MOTOR REMOVAL AND REPLACEMENT

a>

b)

c>

d)

e>

f>

g)

h)

i>

j)

With the access door open and the sealed head removed, isolate the machine from the electrical supply.

Loosen the M3 grub screw in the side of the taper, pull the drive taper off the motor shaft. Remove “V” ring seal from the motor shaft. Do not lose the key in the motor shaft, it should be kept for fitting into the new motor shaft.

Remove the four countersunk screws securing the steel fixing plate to the bowl and motor. The bowl is now released from the motor. (Retain these special screws for refitting new motor).

Remove the outer two countersunk screws securing the front of the bowl to the instrument case. (These screws are under the silicone rubber seal behind the nosecone trim; care should be taken not to damage the seal).

Close and secure the access door. (A protecting cover, ie foam, should be placed over the door).

Remove the four screws securing the nosecone to the plinth (base) and carefully pull the nosecone away from the case. The nosecone will fit face down in front of the plinth (place some protection on the bench for the control panel). Disengage the three connectors , remove the M5 earth nut and leads. The nosecone can now be taken from the machine.

Turn the machine upside down and remove the six securing screws from the plinth (base). The plinth can now be tilted up and to one side for removal of the M5 earth nut and leads (up and to the left when the machine is upside down and facing front). The plinth can now be removed.

Loosen the two screws securing the encoder bracket to the guard barrier and slide the bracket back as far as possible, retighten the screws. Release the M2.5 grub screw fixing the encoder disc to the motor shaft and carefully pull the disc off the shaft, after removing the grip ring from the motor shaft.

Remove the nut retaining the motor leads to the guard barrier.

Remove the four countersunk screws that fix the motor housing to the guard barrier, the motor will now be free to remove from the guard barrier once the leads have been desoldered. (When fitting the new motor ensure correct wiring of motor leads).

29

k) The motor is now removed and the motor housing released from the motor by removal of the four countersunk screws.

Fitting the new motor is generally the reverse of the removal, but the following points should be noted:

a) It is important to use the original screwlok screws in and around the motor. LOCTITR SEX1 LOCK HUST NOT COKR INTO CONTACT WITH ANY PLASTIC OR PAINTED COXPONENT.

b) When re-fitting the encoder disc and bracket, the disc should turn freely near the centre of the gap between the encoder heads, and the bracket should be fixed so that the encoder assembly is as close as possible to the edge of the disc without any risk of contact between the two. Refit the grip ring to the motor shaft.

c) When re-fitting the plinth (base).do not tighten the six screws.

The machine is turned upright and the access door opened (manually operate the solenoid to release the catch).

Replace the PTFE seal along the top edge of the nosecone. Care must be taken when re-fi t ting the nosecone that the two lugs fit smoothly under the bowl seal. Loosely fit the two countersunk screws.. Fit the nosecone so that the front lower edge of the nosecone lines up with the front top edge of the plinth (base). Fix the nosecone using the four screws. Do not over-tighten the two countersunk screws in the bowl. Pull the case forward to meet the nosecone and tighten the six screws..

d) Fit the “V” ring seal to the motor shaft with the lip facing down. Push the seal just past the keyway in the shaft. Fit the key into the keyway on the motor shaft. The drive taper is then fitted and fixed 1 mm from the steel fixing plate (the seal will be pushed down into position by the drive taper).

Reconnect the electrical supply and test the functions of the instrument.

(2) LID REMOVAL AND REPLACEMENT

a) Before isolating the machine from the electrical supply open the lid.

b) Remove the right hand screw (looking from the front of the machine) fastening the lid hinge to the pivot (do not lose the spacing washer or the shakeproof washer). Remove the two M3 countersunk socket screws holding the right hand hinge plate to the lid frame and remove the hinge plate. (Support the lid while removing these parts).

30

c) The lid can now be moved to the left and the left-hand pivot will slide out of the bearing block. The lid can WV be dismantled.

d) Replacing the lid is the reverse of the removal.

e) Forward adjustment of the lid is achieved by slackening the two hinge pivot screws and sliding the lid forward or back.

f) To raise or lower the lid loosen the four screws securing the two bearing blocks at the rear of the machine (two either side) and adjust the lid height accordingly. Remember to also adjust the plunger height in order to keep the lid on the seal correctly.

(3) DRIP TRAY AND LID SEAL REMOVAL AND REPLACEMENT -

a) Before isolating the machine from the electrical supply open the lid.

b) Remove the seventeen countersunk screws retaining the seal and the drip tray to the case of the machine.

c) Remove the drive taper from the motor shaft (M3 grub screw) retain the key from the motor shaft. Remove the ‘V’ ring seal from the motor shaft.

d) Remove the four countersunk screws in the clamp plate and remove the plate. The drip tray can now be removed.

e) Inspect the rubber extrusion on the lid of the guard barrier and replace if necessary. When removing only the lid seal, the seventeen screws need to be removed and a sew seal can be fitted around the drip tray, ensure that the lower lip of the seal fits over the case of the machine and that the seal sits properly on the drip tray.

Replacement of the drip tray is the reverse of the removal. The motor shaft ‘V’ ring seal must be fit.ted with the lip facing down, push the seal just past the keyway in the shaft. Fit the key into the keyway on the motor shaft, the drive taper is then fitted 1 mm from the steel clamp plate (the seal will be pushed down into position by the drive taper). Secure the drive taper.

(4) LID DAMPER ADJUSTMENT

a) Remove the drip tray as described in Section 3.

b) The damper is found on the right hand side of the main case.

c) To increase damping , screw the nylon adjuster into the damper body.

31

d) i To remove the damper from the machine, remove the right hand hinge screw fixing the hinge plate to the pivot.

ii Remove the two M5 nuts securing the damper body to the case. The damper assembly can then be removed.

iii The link arm is separated by removal of the retainer on the damper arm.

(5) GUARD BARRIER REMOVAL AND REPLACEMENT

a) Refer to section (l), step a and step c.

b) After closing and protecting the lid, turn the machine upside down and remove the ten (10) M4 screws securing the plinth (base) to the case and nosecone. The plinth will then lift off to one side of the machine.

c) Remove the M5 earth nuts, leads and plugs joining the plinth to the case and remove the plinth.

d) Disconnect the wiring to the motor, optics and out of balance switch.

e) Remove the eight springs supporting the guard barrier (loop a piece of cable through the outer loop of the spring, restrain the machine with one hand and pull the spring free with the other).

f) The guard barrier can now be removed.

g) Replacement is the reverse of the removal.

(6) LID CATCH MECHANISM

a) If the whole assembly is to be removed, the guard barrier has to be removed first (see section (5))

b) Once the guard barrier is extracted, remove the four (4) M3 screws securing the aluminium angle carrying the catch assembly. The assembly can now be inspected.

c) The catch assembly, the microswitch and the solenoid can be removed without removing the aluminium angle but care must be taken when refitting that the components are correctly fitted.

d) In order to function correctly the solenoid plunger must always be fully home when the solenoid is energised, also the catch must clear the lid plunger with sufficient clearance (until it is flush with the side of the lid plunger hole). Once the solenoid is fully home and the catch is flush with the hole sides, the catch must then be free to move 0.5 to 1.0 mm further out.

32

(7) OUT OF BALANCE SWITCH ADJUSTMENT

a) Remove the round access plug on the back of the instrument.

b) The out of balance switch can be seen through this access hole.

cl the switch is mounted on a rectangular plate held by two screws. Loosen both of these screws.

d) One of the screws is in a slot. Slide the rectangular plate counter-clockwise a small amount - approximately loo. Retighten both screws.

e) Test for out of balance using all 12 chambers and clip assemblies. Once you are satisfied the machine does not go out of balance, proceed to step f).

f) Using 10 chambers and clip assemblies, load the sealed head (see Operator Manual). Ensure that the two positions left vacant are not directly opposite each other.

Select high acceleration and 2000 rpm and start machine. The out of balance should operate at a point between 1000 and 1500 rpm.

Repeat using low acceleration.

g) Make certain the screws are tight; replace the access plug and retest.

(8) POWER SUPPLY REMOVAL AND REPLACEMENT

a) Isolate machine from the electrical supply.

b) Protect the lid (use foam etc).

c) Turn the machine upside down and remove the ten (10) M4 screws.

d) Lift the plinth up and to the left side (looking from the front). Disconnect the 3 plugs and ground leads, and the plinth is then free to remove.

e) Disconnect the electrical leads from the power supply, and supporting the plinth and power supply, remove the four fixing screws from the underside of the plinth. The power supply is now free of the plinth.

f) Replacement is the reverse of the removal instructions.

33

(9) HEATSINK

Two different types of heatsink may be found

a) Internal b) External

a) Internal

i> Remove the plinth [as in Section 8, paragraphs a) to d)].

ii) Disconnect and remove the heatsink retaining nuts, desolder the transistor leads and disconnect the wiring fixed to the plinth. The plinth can now be removed.

b) External

i> Remove the two outer M3 screws and extract the heatsink from the machine.

ii) The transistor mounting body is secured using two M3 screws. Remove these and the transistor can be seen. Desolder and remove from the machine.

iii) In both cases the replacement is the opposite of the removal.

(10) NOSECONE ASSEMBLY

Removal and Replacement Main PCB

a) Remove the nosecone, (see Section 1, paragraphs a, d and f).

b) Disconnect the main PCB from its retaining pillars, and partially extract it through the back of the nosecone. Take care not to damage any of the components on the board.

c) Disconnect PCB plugs.

Remove the main PCB.

Replacement is the reverse of removal.

NB: Replace the PTFE seal along the top edge of the nosecone.

Proximity Switch

a> Remove the nosecone (Section 1, paragraphs a, d and f).

b) Remove the main PCB.

34

c> Remove the two M4 screws retaining the nosecone trim and remove the trim.

d) Remove the two M3 nuts and remove the proximity switch.

Note: It is possible to remove the trim.without removing the main PCB using a screw retaining screwdriver, but great care must be taken to avoid damage to any components on the PCB.

Replacement is the reverse of removal, but when positioning the proximity switch it must be adjusted so that the machine will not start when the lid is slightly open. The switch must be out of the magnetic field before the lid plunger releases the lid catch microswitch.

NB: Replace the PTFE seal along top edge of the nosecone.

Front Panel

a) Remove the nosecone (Section 1, paragraphs a, d and f).

b) Remove the main PCB.

c) Remove the eight (8) M3 nuts and remove the front panel from the front of the nosecone.

Replacement is the reverse of removal.

NB: Replace the PTFE seal behind the front panel. Also replace the PTFE seal along the top edge of the nosecone.

35

PARTS LIST IDENTIFICATION CYTOSPIN 3

Ident No Part No Description From Ser No Issue

1 59930096 Top Cap Locking Pin MA 1000 07P

2

3

4

5 G, 4

6

7

8 PO9698 Thumb Nuts (2)

9

10

11

59930097

PO7595

59930095

Handle - Sealed Head Assembly

Seal - Silicone - Sealed Head

Locking Pin - Locking Device

PO7437 Top Cover Mouldings

59930094

PO9638

Main Body - Locking Device

Ball Bearing %" dia (2)

59920047

PO7431

59930093

Cell Plate

Seal V Ring

Hub Sealed Head

1

3

1

5

1

5

1

1

3

1

4

PARTS LIST IDENTIFICATION


12

13

14

15

16

2;;:

17

18 59930068 Fixing Collar

19

20

21

22

PO6007

PO7525

PO7516

59920046

59930063

PO7432

PO7596

59920033

PO7512

PO7402

Grub Screw M3 x 8 (2) MA 1000 07P

0 Ring

Seal

Inner Bowl

Drive Boss

V Ring - Motor Shaft

Moulded Seal

Drip Tray

Key 2 mm x 2 mm x 12

Motor

1

1

1

1

4

1

4

1

2

1

1



23 59920034 Outer Bowl MA 1000 07P 7

24 PO9610 Spring Retention Pin (8) II 1

25 PO7441 Spring Barrier (8) 11 1

26 59920038 Bracket - Vibration Switch 11 2

27 59930072 Clamp Plate

28 PO7524 Mercury Position Sensor

29 59930070 Support Pillar (3)

30 59930064

31 59930059

32 PO7444

33 59930060

Support Collar

Carrier Plate

Encoder Disc

Clamp Plate

11 1

II 1

II 2

II 5

II 2

11 1

II 1



34

35

36

36

37

&

37

38

39

40

41

41

PO7522

59920127

PO7467

59920131

59930154

59930155

59910131

PO6979

59930051

PO4249

59930162

Grip Ring MA 1000 07P

Mounting Bracket II

Opt0 Interrupter (2)

Opto Interrupter PCB

Angle Bracket Right

Angle Bracket Left

Motor Suppresser Plate

12 Way Receptacle Housing

Mounting Block

5 mm Nut

Locknut

i

1

1

2

1

1

2

1

1

1

3



42

43

44

45

45

s

46

47

48 59930152 Hinge Block - Right

48 59930167 Hinge Block - Left

49

50

59930161

74010051

74030053

74030052

74030051

59930071

59930055

59920032

PO7430

Plunger Lid Catch MA 1000 07P

Lid Panel Assembly 11

Screw - Hinge Plate (2)

Hinge Plate - Right Hand

Hinge Plate - Left Hand

Tapping Strip (2)

Hinge Pivot

Damper Arm Assembly

Rubber Buffer

II

II

II

11

I,

II

II

II

II

2

1

1

1

1

4

2

3

3

5

1



51 PO9696 Damper Rod MA 1000 07P

52 PO7426 Damper I,

53 PO7763 Plug Cover (Mercury Switch) II

54 74020030 Maincase ,I

55 59930044 Guide Bracket 11

56 59930160 Mounting Angle II

57 59930042 Mounting Plate Solenoid II

58 PO6741 Plug 318" Black II

59 PO7526 Spring Solenoid II

60* PO6148 Solenoid II

* NOTE: Items 60 and 61 are bonded together and should not be ordered separately.

1

1

1

1

1

1

1

1

1

5



61* 59930043 Adaptor - Solenoid - Spindle Catch MA 1000 07P 3

62 , 59910121

63 PO7764

64 59930037

67

68

69

70

76

77

59920128 Proximity Switch Assembly

PO2969 Grommet

P11993 Proximity/Switch Pillar

PO7476 Voltage Regulator

74030070 Top Trim

74020061 PCB Mainboard

74020064 EPROM for above

Items 60 and 61 are bonded together and should not be ordered separately. * NOTE:

Catch Assembly

Microswitch

Microswitch Bracket

II

I,

,I

II

,I

,I

,I

I,

II

II

4

1

3

1

1

1

1

1

1

1



79

81

82

83

84

z

85

86

89 59920009 Heatsink

90

91

92

74020060

74030020

PO6979

PO6976

PO9407

PO9254

59930005

74020001

PO1517

PO7882

Nosecone

Front Panel Cytospin 3

Receptacle 12 Way

Plug Housing 12 Way

Transistor PMD16K60

Transistor Mounting Kit

Transistor Mounting Block

Plinth

Clamp Plate - Mains Lead

Fuse Holder

MA 1000 07P i

11 1

II 1

II 1

‘1 1

'1 1

I' 1

1' 3

II 1

II 1

II 1



95 PO6755

96 59930009

97 PO7155

98 PO6102

99 PO7462

100 59920041

93

94

PO4654

P11130

PO7884

Fuse Marking Plate MA 1000 07P

Fuse 0.8 Amp Slow Blow

Fuse Cap 240V FEIT 031-1663

PO7828 Fuse 2 Amp Slow Blow

PO7883 Fuse Cap 1lOV FEIT 031-1661

Input Block Mains

Terminal Block Pad

Relay 60 73 IML

Capacitor Clip

Capacitor 4700 yF

Tagboard Assembly 240V

II

II

II

II

‘1

II

‘1

1

1

1

1

1

1

1

1

1

1

5



100

101

102

103

104

105

106

107

108

59920043

P11944

PO6979

P11109

59930001

PO7807

59930116

59930171

PO7602

59930156

Tagboard Assembly 1lOV

Rocker Switch

Receptacle 12 Way

Mains Filter

Transformer Plate

Diode Bridge Rectifier

Transformer

Spacer

Black Neoprene Buffer 1%" x 'h" Thick

Hinge Adjustment Screw

MA 1000 07P

II

‘1

‘1

II

4

1

1

1

4

1

1

2

1

2

RECOHMENDEDPIRST LINE REPAIRKIT - CYTOSPIN 2

Part No

59920041 59920043 59920131 59930094 59930095 59930096 59930097 59930116 74010004 74010005 74020061 74020062 74020063 74020064 74030020 PO5606 PO6148 PO7155 PO7402 PO7431 PO7432 PO7437 PO7444 PO7462 PO7467 PO7476 PO7516 PO7524 PO7525 PO7595 PO7596 PO7763 PO7764 PO7807 PO9254 PO9351 PO9407 PO9638 P11109 P11944

Description

Tagboard Assembly 240V Tagboard Assembly 1lOV Opto PCB Main Body - Locking Device Locking Pin -. Locking Device Top Cap - Locking Pin Handle - Sealed Head Transformer Transformer Plate Assembly 1lOV Transformer Plate Assembly 240V PCB Mainboard Programmed PAL Programmed PAL Programmed Eprom Front Panel Proximity Switch (SW3) Solenoid Relay - LO 73 IMO Motor - Type 55M Spec Seal - V Ring Seal - V8 - Motor Shaft Top Cover Moulding Disc Encoder Capacitor 4700 nF Opt0 Interrupter Voltage Regulator Seal Switch Mercury (SW4) '0' Ring Seal - Silicone - Sealed Bead Seal Moulded Plug and Cover - Mercury Switch Microswitch (SW5) Diode Bridge Rectifier Mounting Kit Brush Assembly - Motor Transistor - Darlington - PMB 16K60 Ball Bearings Mains Filter Switch - Rocker (SWl)

47

10 LIST OF DRAWINGS

Page No

Cytospin 3 llOV-250V Circuit Diagram E740-1-l 49

Circuit Diagram - Main PCB F740-1-2 50

Note: The PCB used on the Cytospin 3 is a common board, not all component locations will be used.

48

110 ? a I n I

I’ I I I - I I

CIRCUIT DIAGRAM-MAIN PCB F740-1-2

SHANDON SOUTHERN PRODUCTS LTD CYTOSPIN III

40 41 42 43

108

28 26 27

I :

T

I

-

I

PLGh SK11

--

- GY

RD

GYM

, GYM CY@K

1 WTlcY

z-w)--

3-m) WTXXI

4-m) W-MN

5-W)--

6--m)--

7-r )--

8-w)--

9-m)---

lo-==>-

11-m)--

12-my ) WTM

PL2%

1 f: WTNL

2 RD

RD RD

GY GY

RD RD

BK BK

BK BK

, , RD

GY 0 RD 1 -3

GY@K _ RD tl

1-L

Cl

-7 47mtJf -*

I 1 ’ I

GYM I

I- -------

[GYBK I

PLGI SK12

4 '

51 -RD I

I

I f

I I

RIA 3&; 1 WXN 1 I

L-cl 4 5.a --

1 8 p --

10 11 - -

4

5

6

i 1 0

9

10

11

I2

1 I t

SENSOR P.C.B.

wlm w4>--

i

5 ) WTrcR

WlflD 6)

WTIRD

7 )--

I wT/8N ln2 VIBRATKX WTBL SWITCH

WTBK 1 SW4

LIDLOCKEDSWIICH SW5

CIRCUIT DIAGRAM-CYTOSPlN(llO-250V)

APPENDIX

PRINCIPLES OF OPERATION

This description uses drawing numbers:

F740-1-2 Circuit Diagram Main PCB E740-l-1 Circuit Diagram Cytospin (110-250V)

(1) OVERVIEW OF SYSTEM

The Cytospin 3 is a bench-mounted centrifuge with a range of speeds from 200 - 2000 rprn. The time and speed required are entered and displayed digitally by means of a keyboard and LED displays.

The Cytospin 3 incorporates a number of safety features:

1 Overspeed detection 2 Out of balance detection 3 Electronic failure detection

The Cytospin 3 has been designed to provide maximum protection to the operator from biological specimens.

(2) GENERAL (Ref F740-1-2)

The Cytospin 3’s electronic circuit occupies one PCB in the nosecone assembly. It is connected to the main instrument by means of a detachable loom. This PCB replaces the earlier versions as fitted to the Cytospin 2 and is not retrofittable.

The PCB is partitioned into well defined functional areas which are described below. The PCB is factory calibrated and requires no analogue setting-up procedure.

(3) POWER SUPPLY

Mains power is supplied to the machine via SW1 (the main on/off switch) which is mounted on the plinth. The internal circuitry is protected by FSl, a slow-blow fuse, 0.8A for 250V operation - 2A for 1lOV operation. Interference protection, both from interference getting into the machine from the external mains supply and vice-versa, is provided by FLl.

The low voltage supplies for the machine are derived from Tl, this is a toroidal transformer to minimise space and temperature rise. It has two primaries which are connected in series for 240V operation or in parallel for 1lOV operation, as shown on drawing E740-1-l. Two voltage dependent resistors Sl, S2 are connected across the primaries to suppress any voltage spikes which are not attenuated sufficiently by FLl. Tl has three secondaries, 24V AC at 4A rms, 8-0-8V AC at 2A rms and 15V AC at 0.58 rms.

51

The 24V winding is used to supply power to the motor, lid solenoid and motor control relay, it is rectified by diode bridge Bl and smoothed by Cl, a 4700uF capacitor. Both Bl and Cl are situated on the transformer plate assembly in the plinth.

From the transformer, 8V AC is fed to the main PCB, rectified by Dll, D12 and C36, then regulated by VR3 to provide a +5V supply * The 12V AC winding is unused on the Cytospin 3.

(4) MICROPROCESSOR UNIT

The microprocessor system on the Cytospin 3 is centred around IC6, a Hitachi HD63A03Y 8-bit microcomputer. The clock signal for the microcomputer is provided by the 4.9152 Mhz quartz crystal at X2 together with capacitors C245 and C25.

The system ROM is contained in IC7 while the PLA at IC8 is used for address decoding. In the event of a power failure, all stored programs are retained in memory by the EEPROM at IC4. Power failure detection and software watchdog facilities are provided by the MAX690 at IC2 and the PLA at IC5 respectively.

(5) DISPLAY AND KEYBOARD CIRCUITS

The display and keyboard driving is multiplexed. The LED’s 1 - 8 are scanned by a walking zero on port six of the microcomputer, as are the seven segment displays. This allows for increased intensity of display for reduced current consumption.

The walking zero is used to switch on one of the transistors T16 to T23. The transistor supplies the common anode of the seven segment display and another LED with 5V. The other end of the LED’s in the seven segment display and the separate LED are pulled low through RN2 and R41. This is done selectively by the pattern being fed through from the darlington driver chip ICll.

ICll is memory mapped and driven by the processor to power the correct display segments. All the seven segment displays are mounted in tuned pin sockets for easy replacement.

The keyboard is of standard touchpanel construction being arranged in three rows of eight. The rows of eight going vertically down the keyboard are scanned by port 6 of the microcomputer at a frequency of about 100 Hz, therefore response is immediate.

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The three horizontal rows are monitored by IC13 which is memory mapped. Connection to the keyboard is through a single in line 18-way Berg connector (PL3) via a short tail to the top of the keyboard.

LED 9 is driven by IC14 and supplied from pull up resistor R40.

(6) MOTOR DRIVE CIRCUIT

The motor control output provides a regulated current to the base of the motor drive power darlington transistor TRl.

v This current is approximately -CD

- 1.5

300 amp, where V,, is the

voltage at the collector of transistor Tll and 300 is the value in ohms of R28. V,, is controlled by a unity gain long-

tailed pair amplifier made up from T8, T9 and TlO. The amplifier compares the collector voltage V,, with an input

taken from a reservoir capacitor C26 to adjust the base current of Tll. The voltage appearing at capacitor C26 is controlled by a series of one millisecond pulses which are generated at pin 14 of the microprocessor. These pass through inverter IC8c and a resistor R27, which provide the correct charge level and rate control for the capacitor.

(7) SOLENOID AND RELAY DRIVE CIRCUITS

The solenoid which is activated to allow the lid to be opened and the relay which is driven to allow motor current to reach the motor both operate from +24 volts and have similar drive circuits. Because of the inductive nature of both devices, by-pass diodes D6 and D7 are fitted to re-circulate switched current.

The solenoid is activated by taking IC6 pin 23 low and the relay by taking IC6 pin 15 low. In each case this switches on a pnp transistor (T15 and T13) connected to +5 volts, which provides base current to an npn transistor (T14 and T12), which itself powers the device. The base current to the drive transistor in each case can be clamped to ground to prevent the transistor switching on. For the relay, this is done using an open collector handgate IC17 driven by KEs and mRT providing a safety interlock.

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The lid release solenoid can only be driven under software control. Should the software control fail, the open lid key will not operate. In an emergency the lid release solenoid can be opened manually. This is done by turning off the mains supply and inserting a thin screwdriver through the hole provided in the left side panel. The hole is covered by a plastic cap.

(8) SPEED WATCHDOG AND OUT OF BALANCE

The out of balance detector mercury switch is used to generate an mAT by being latched on the NM1 decode/general interface PAL (IC5). This will cause the drive to the motor to be cut via IC17 and the plinth relay to release, causing regenerative braking.

In addition, the BAL LED will light showing an extreme fault condition. The Cytospin 3 has to be switched OFF then ON to recover from the situation. Other signals that cause the same sequence of events include hardware overspeed. The ability to detect imminent power fail is not used.

The Cytospin 3 has a software based overspeed detection circuit that will cut the drive, cause regenerative braking and light the ! LED. Recovery again is by switching OFF then ON.

The microprocessor monitors the angular speed by monitoring the output of the slotted opto mounted beneath the motor. This signal is fed to the board PL2a pin 3 then squared up using the Schmitt inverter IC18 and fed into the microprocessor’s interval timer input on IC6 pin 9.

Running in parallel to this software overspeed detector is a true hardware overspeed detector. This circuit is comprised of IC3 and its associated discrete analogue components. Essentially the circuit is a frequency to voltage converter.

The squared up opto signal is differentiated using C18, IC3 and R3 to produce a pulse for every edge detected. This pulse is then rectified using D2 and used to charge C19, should the speed be too high Cl9 will be unable to discharge to a low voltage. The average of the voltage on Cl9 is generated across C20 using R4, it is this voltage that is a reflection of the speed.

The final stage using POT2 and IC3 is a comparator producing a low when overspeed is detected. This signal is latched by the PAL IC5 creating an NHI and the usual machine shutdown. POT2 is used to set the voltage threshold and hence the frequency threshold. Due to the variation in component value and the variation in the Vf of D2, the circuit must be calibrated using a known frequency of 2.6 KHz on the spin input to produce the overspeed warning.

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The circuit once calibrated should never require recalibration. A feature of the software is the anti-jam mechanism. This checks to see if any pulses are being generated on the rotor speed opto interrupter input line. If the microprocessor expects rotor rotation because power is applied to the motor and none is detected, then drive power will be removed after a period of approximately 4 seconds. This is to prevent motor damage if the motor is jammed, but also acts as a safeguard against opto interrupter failure.

(9) NMI, RESET, HARDWARE WATCHDOG AND OPEN COLLECTOR LOGIC

The FU3% has the same action on the open collector logic as the NRT signal. The two signals being added together in IC8 to produce the CVT signal driving IC17, cutting drive and driving the plinth relay RLl via T14 to cause motor drive isolation and regenerative braking.

The m signal will be produced by the MAX690 (IC2) in one of two situations. Should the power fail, prior to the ILogic levels being affected, at 4.5 volts the microprocessor is reset and will not be released from reset until such time as the power rail has risen above 4.5 volts and a further 1.6 seconds has elapsed.

The NRT signal will also be produced should a watchdog signal not be produced on IC6 pin 22. This signal is an indication that the software is operating normally, its absence indicates software corruption.

(10) MOTOR, OPT0 DISC, LID LOCK AND BALANCE SWITCH

The motor used is a 24V permanent magnet type, mounted in line with the rotor assembly. The power to the motor is controlled by relay RLA and transistor TRl (E740-l-l), these components are mounted on the plinth. When the machine is stopped or de-energised, power to the motor is removed and it is short circuited by contacts RLAl, RLA2 (connected in parallel) of relay RLA. Thus, if the machine is stopped the motor is regeneratively braked (ie it acts like a generator with a short circuit output). When the machine is started RLA is energised and power can be supplied to Ml via TRl. Because this transistor under certain conditions is called upon to dissipate up to 6OW, it is mounted on a heatsink which projects out of the rear of the machine. The control signal for TRl is derived from the main PCB and is described above.

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The lid of the Cytospin is locked by means of a mec.hanical latch which can only be released by means of a solenoid Sol 1 (E740-l-l), the control signal for which comes from the main PCB; So as to inform the microprocessor control system if the lid is locked or not, two switches are incorporated in the circuit, SW3 and SW5. Switch SW3 is a reed relay proximity switch which is activated by a magnet in the lid. The switch itself is mounted in the trim on top of the nosecone. As the lid is closed the magnet is brought closer to SW3 and when it is within range the switch will close. To ensure that the lid is fully locked, a microswitch is incorporated in the lid latch assembly (SW5), this switch detects if the lid latch is not fully home. Since SW3 and SW5 are in series, a signal (5V) will only be present to the main PCB when the lid is fully shut (F740-l-l).

In the event that the head is loaded with a non-balanced load and the machine started, an out of balance switch is incorporated to inform the microprocessor to switch the machine off. This switch, SW4, consists of two contacts situated above a small bead of mercury. If the machine vibrates the mercury is agitated and bridges the gap between the contacts, this signal is latched on the main PCB and stops the machine. Because the mechanics of the suspension system exhibit different characteristics depending upon whether the rotor is accelerating or decelerating, the switch is then taken out of the circuit by RLA3 when the motor is turned off.

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cytospin 3 (74010101 iss. 02) - udh.med.sa · the service manual for the cytospin 3 bench top...

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