cytoperm & bbd 6220

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B/c

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CYTOPERM® 2CO2- AND CO2/O2 INCUBATOR

Contamination protection that’s in a class of its own

CYTOPERM® 2CO2- AND CO2/O2 INCUBATOR

Quality Products – Lifetime Care

CYTOPERM® 2 GASSED INCUBATOR – CELL CULTIVATION FOR THE 21ST CENTURY

Using our experience combined with latest technologies and practicerelated feedback from our customers,we have developed this unique CO2incubator offering 180 °C disinfection.

Applicationscytoperm® 2 meets stringent require-ments essential for research in pharma-ceutical, cancer, AIDS, vaccine produc-tion and IVF fields and other applicationsinvolving sensitive or infectious samples.

Cells and tissues, which should grow in vitro, must find comparable in vivoconditions in the cell culture. cytoperm® 2 controls temperature, CO2 content and relative humidity withexceptional precision to simulate thenatural environment of cells. As an option cytoperm® 2 can also control the O2 concentration.

The incubator's benefitscytoperm® 2 represents the synergies ofsafety and reliability for cell and tissueculture applications ■ hot air disinfection at 180 °C,

including all fittings and sensors ■ pyrolytic germ barrier for sterilization of

the humidification water■ water reservoir conveniently located

outside work space ■ humidity control■ thermo jacket heating system

CYTOPERM® 2 GASSED INCUBATOR – CELL CULTIVATION FOR THE 21ST CENTURY

The ultimate equipment:cytoperm® 2

The Heraeus® gassed incubator cytoperm® 2 offers simple handling,high protection against contami-nation and safe operation.

Disinfection routine with temperature and status display

Contamination preventionContamination by bacteria, viruses,fungal spores and mycoplasmas candestroy valuable cultures or distort testresults, causing more work. Thecytoperm® 2 gassed incubator's designincorporates measures which avoid orefficiently eliminate contamination. Thesemeasures include, for example, theinterior's design just like the automaticdisinfection routine.

180 °C hot air disinfectionA heating system operating independentlyof the incubation system raises thetemperature of the work space to 180 °Cfor disinfection. The automatic routine isinitiated by a simple key switch and canbe repeated as often as required. Allfittings and sensors remain inside theincubator during disinfection. A GLPtested laboratory, accredited in accor-dance with DIN EN 45001, confirms the180 °C hot air disinfection routine'sefficiency.

Test germs to varify the efficiency:■ Bacillus subtilis■ Bacillus stearothermophilus (USP 23)■ Enterococcus faecalis■ Escherichia coli■ Pseudomonas aeruginosa■ Aspergillus niger

Pyrolytic germ barrierWater for humidification always passesthis barrier and is sterilized at 500 °Cbefore entering the incubator. Thissystem reliably prevents spread ofcontamination.

External water reservoirThe water reservoir of the cytoperm® 2 is located outside the incubation chamber,thus there are no open water surfaceareas inside the incubator. Combinedwith the pyrolytic germ barrier, contami-nation is effectively counteracted.

Gas tight screenSix individually sealed glass doors whichallow segmented access to individualsections of the incubator are provided asstandard. This minimises any changes tothe atmosphere during opening, shor-tens recovery times significantly and alsofurther reduces the risk of contamination.

Air jacket heating systemThe air jacket heating system maintainsconstant and stable temperatures on allinterior surfaces which ensure a conden-sation free environment.

PROTECTION FOR HIGH DEMANDSPROTECTION FOR HIGH DEMANDS

Easy to clean interior

Lockable heated door and waterlevel indicator for the waterreservoir

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Reliable sensors with automatic cali-bration ensure the long term stability ofincubation parameters.

Constant environmental parametersThe cell's environmental conditionschange, for example, when the gassedincubator is opened. Permanent controland regulation of the parameters ensurethat such changes are detected and therequired in vitro conditions are re-established in the shortest possible time.This feature ensures the high degree ofstability in environmental conditionsrequired for both long and short termcultures.

Temperature controlTemperature is microprocessor controlled with a Pt 100 sensor.

Humidity controlThe relative humidity (rH) is controlledwith a microprocessor. The maintenancefree sensor operates in accordance withthe capacitive humidity measuring princi-ple. The water reservoir for humidificationis located outside the work space and iseasy to fill, empty and monitor.

CO2 control using auto-zeroCO2 levels are microprocessor controlledusing a thermal conductivity sensor withexcellent long term stability and reliabilitywith built-in humidity compensation. Afully automated calibration (auto-zero) iscarried out periodically to ensure CO2long term stability and thus stable CO2levels and a constant pH level in culturemedia with bicarbonate buffers.

IDEAL CULTURE CONDITIONS

HIGH SAFETY STANDARDS

IDEAL CULTURE CONDITIONS

The cytoperm® 2 offers a number ofsafety features:

auto-start functionThe auto-start function, which consider-ably simplifies the equipment's operation,contains the incubator's automatic start-up and the measuring systems' calibration.The incubator can be loaded immediatelyafter the start-up routine is completed.

Locking of set valuesBy locking the set values, unauthorisedalterations of the incubation conditionsare prevented. Switching the cytoperm® 2on and off and setting off the disinfectionroutine is done via a key switch.

Overtemperature protectionA second, totally independent controlsystem with an additional Pt 100 temperature sensor protects samplesfrom overtemperature.

Alarm and error diagnosisAlarm functions are provided for allcontrol parameters, giving acoustic orvisual signals when errors occur. Theerror diagnosis system identifies mal-functions, which can be queried usingthe ”i“ (information) key on the operatingpanel.

Lockable doorUnauthorised access to samples can beprevented through the lockable door.This feature is particularly importantwhen dealing with hazardous samples orduring long term experiments.

Safety during power failureAll operating parameters remain stored inthe event of a power failure. When poweris restored the unit automatically returnsto standard operation and immediatelyreinstates the set parameters.

HIGH SAFETY STANDARDS

The clear operating panelensures simple handling

Potential free contact and RS 232 interface

TECHNICAL DATA

Type Unit Value/Description

Dimensions External casing (w/h/d) mm 920 x 855 x 775Work space (w/h/d) mm 607 x 669 x 585Total volume l 220

Shelves, two piecesDimensions (w/d) mm 260 x 500No. standard/maximum 6/16Max. load per shelf/total load per unit kg 5/30

Access portAccess port Ø 20 mm rear wall bottom left

MaterialWork space and fittings stainless steel

Start-up using auto-start routineStart-up time at 37 °C set temp. h ca. 4.5

Disinfection routine verified by an accredited laboratoryDisinfection temperature on all surface areas °C/h 180/3Disinfection routine h ~12Efficiency spectrum bacteria, fungi, spores (USP 23)

Temperature thermo jacket heating systemMeasurement and control range CO2 unit °C TA

1) + 5 … 50CO2/O2 unit °C TA

1) + 7 … 50spatial2)/temporal2)3)

Temperature deviations K ≤ ± 0.1/≤ ± 0.5Heating up time to 37 °C with auto-start4) h approx. 5Ambient temperature range °C 18 … 30Recovery time5) min ≤ 3

Humidity external water reservoirControlled, measuring principle rH capacitive humidity measurementControl range % rH 60 … 95Control accuracy % rH ± 1Recovery time at 95% rH2) min ≤ 9

CO2 TCDControlled, measuring principle thermal conductivity measuring cell with

auto-start and auto-zero functions, 180 °C hot air disinfection

Measurement and control range vol % 0 … 20Control accuracy vol % 0.1Recovery time at 5% CO2

2) min ≤ 2

O2 control (option)Controlled, measuring principle zirconium oxide sensor with

auto-cal functionControl range % O2 3 … 90Supply of O2 for the range % O2 > 21 … 90Supply of N2 for the range % O2 3 … < 21Control accuracy % O2 ± 0.5Recovery time at 7% O2

2) min ≤ 15

Electrical dataRated voltage V~ 230Rated power

incubation operation at 37 °C kW 1.2disinfection operation at 180 °C kW 1.2

Rated frequency Hz 50/60

Weight (excluding accessories) kg 1071) TA = ambient temperature 4) TA = 22 °C, incubator empty2) DIN 12880, part 2/11.78 5) at 37 °C, after 30 sec open door, to 98% of the initial value3) at 37 °C

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ngMonitoring and documen-tationShould any malfunction occur,cytoperm® 2 conveys theinformation directly to a centralmonitoring system via a potentialfree contact. The Heraeus®

cytoperm® 2 is equipped withan RS 232 digital interface formonitoring and documentationof the operating parameters.The documentation softwarecomes standard with thecytoperm® 2.

cytoperm® 2 CO2/O2

This model has been designedfor cell cultivation under physio-logical and tissue typical oxygenpartial pressure conditions. The zirconium oxide sensor formeasuring oxygen is main-tenance free, can be disinfectedat 180 °C and is automaticallycalibrated (auto-cal). By supply-ing oxygen or nitrogen, the O2 content can be controlledfrom 3–90%.

TECHNICAL DATA

Operating panel of the cytoperm® 2with additional O2-control.

SERVICE FOR OUR CUSTOMERS

ORDER NUMBERS

cytoperm® 2, CO2 51011659

cytoperm® 2, CO2/O2 51011660

Accessories

Support frame, height 300 mm 50031348Support frame, height 780 mm 50029597Rack to stack two cytoperm® 2 50053628Additional stainless steel shelf, half width including 2 shelf supports 50029943Gas cylinder monitor with acoustic signal, 120/230 V~, 50/60 Hz 50046033CO2 cylinder pressure regulator 03429937N2 cylinder pressure regulator 03429942O2 cylinder pressure regulator 03429943

Your benefits at a glance■ 180 ° disinfection routine■ external water reservoir■ optional O2-control■ unique warranty and service

ORDER NUMBERS

Your Sales Contact

SERVICE FOR OUR CUSTOMERS

Your regional contact is at your disposal for fast service.

Australia Kendro Laboratory Products · Lane Cove, Sydney · NSW 2066 · Tel. +61 (2) 9936 1540 · Fax +61 (2) 9427 9765 · [email protected] Kendro Laboratory Products GmbH · Vienna · Tel. +43 (1) 801 40-0 · Fax +43 (1) 801 40 40 · [email protected] Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. 1 (203) 270-2080 · Fax 1 (203) 270-2166 · [email protected] Kendro Laboratory Products Beijing Rep. Office · Beijing · Tel. +86 (10) 6501-1573 · Fax +86 (10) 6501-4229 · [email protected]

Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. +852 2711-3910 · Fax +852 2711-3858 · [email protected] Laboratory Products Shanghai Rep. Office · Shanghai · Tel. +86 (21) 5490-0216 · Fax +86 (21) 5490-0230 · [email protected]

Denmark Kendro Laboratory Products AB · Albertslund · Tel. +45-43 62 46 47 · Fax +45-43 62 46 41 · [email protected] Kendro Laboratory Products · Courtaboeuf cedex · Tel. +33 (1) 69 18 77 77 · Fax +33 (1) 60 92 00 34 · [email protected] Kendro Laboratory Products GmbH · Hanau · Tel +49 (1805) 536 376 · Fax +49 (1805) 112 114 · [email protected] Kendro Laboratory Products (India) Pvt. Ltd. · New Delhi · Tel. +91 (11) 618 48 40 · Fax +91 (11) 618 53 97 · [email protected] AHSI S.p.A. · Cavenago Brianza · Tel. +39 (02) 95 08 11 · Fax +39 (02) 95 08 12 77 · [email protected] Nippon Kendro Co. Ltd. · Tokyo · Tel. +81 (0) 3 3517-1661 · Fax +81 (0) 3 3517-1664New Zealand Kendro Laboratory Products Pty Ltd · Auckland · Tel. +64 (9) 525 03 33 · Fax +64 (9) 525 03 37 Poland Kendro Spólka z.o.o. · Warsaw · Tel. +48 (22) 663 43 23 · Fax +48 (22) 663 43 25 · [email protected] Heraeus S.A. · Cacem · Tel. +351 (1) 912 08 65 · Fax +351 (1) 912 08 60 · [email protected] Heraeus S.A. · Madrid · Tel. +34 (91) 358 19 96 · Fax +34 (91) 358 20 67 · [email protected] Kendro Laboratory Products AB · Sollentuna · Tel. +46 (8) 585 777 50 · Fax +46 (8) 623 15 45 · [email protected] Kendro Laboratory Products AG · Zurich · Tel. +41 (1) 454 12 12 · Fax +41 (1) 454 12 99 · [email protected]

Kendro Laboratory Products SA · Carouge-Genève · Tel. +41 (22) 343 21 67 · Fax +41 (22) 342 38 31 · [email protected]./Ireland Kendro Laboratory Products Ltd. · Bishop’s Stortford · Herts · Tel. +44 (1279) 827700 · Fax +44 (1279) 827750 · [email protected] Kendro Laboratory Products · Newtown, CT · Tel. +1 (800) 522-7746 · Fax +1 (203) 270-2166 · [email protected]

All other countries in Asia Pacific North Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. +852 2711-3910 · Fax +852 2711-3858 · [email protected] Pacific South Kendro Laboratory Products · Lane Cove, Sydney · NSW 2066 · Tel. +61 (2) 9936 1540 · Fax +61 (2) 9427 9765 · [email protected], Middle East, Africa

Kendro Laboratory Products International Sales · Hanau · Germany · Tel. +49 (1805) 536 376 · Fax +49 (1805) 112 114 · [email protected] America Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. +1 (203) 270-2080 · Fax +1 (203) 270-2210 · [email protected]

Internet http://www.kendro.com

Quality Products – Lifetime Care

Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards: CE for the European Union, and UL, cUL or CSA standards forNorth America. ©2001 Kendro Laboratory Products. All Rights Reserved. Printed in Germany 2C 05/01 VN 4t Druckerei

For Ordering or Technical Information

BBD 6220 CO2 INCUBATORWITH AUTOMATIC 180 °C HOT AIR DISINFECTION

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B/B

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BBD 6220 CO2 INCUBATORWITH AUTOMATIC 180 °C HOT AIR DISINFECTION

BBD 6220 CO2 INCUBATOR –CONSISTENT SAFETYBBD 6220 CO2 INCUBATOR –CONSISTENT SAFETY

For the past 20 years HeraeusInstruments has led the field in thedevelopment of CO2 incubators with180 °C hot air disinfection. Nowexpertise, innovation and excellenceare brought together in the latestaddition to our product range – thenew BBD 6220.

With the BBD 6220, theseadvantages are assured:■ hot air disinfection at 180 °Cwithout removing and replacingsensors and fittings■ no water storage within theworking space■ sterilisation of water beforeworking space is humidified

Protection and precisionThe BBD 6220 offers exceptionallyaccurate control of temperature, relativehumidity and CO2 level, creating stableambient conditions to ensure in vitro cellgrowth.

Reliable protection against contaminationis ensured by the smooth surfaces ofthe stainless steel interior and the waterreservoir being situated outside theusable working space. In addition, theautomatic 180 °C hot air disinfectionsystem ensures safe destruction ofviruses, bacteria, mycoplasma andfungi.

The BBD 6220 is GS tested.

The BBD 6220 setsnew standards.

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THE BBD 6220 – A RANGE OF BENEFITS

Fully automatic 180 °C hot air disinfectionThe interior can be automaticallydisinfected at 180 °C without the needto remove fittings and sensors. A statusdisplay indicates whether the unit is inthe heating up, disinfecting or coolingdown phase. Following disinfection, theBBD 6220 automatically returns toincubation mode.

Controlled humidityWater is sterilised before it reaches theworking space to provide humidity. Thewater reservoir is easy to fill and emptyand is situated outside the usableworking space. Relative humidity (rH) isadjustable.

Overtemperature protectionTo safeguard samples from overtempe-rature, there is a second totally inde-pendent controller with its own tempe-rature sensor.

Easy cleaningThe rounded corners and smoothsurfaces of the stainless steel interiorensure easy cleaning.

Condensation-free interiorThe hot air jacket heating system ensuresthat the interior and wall temperaturesremain stable. Even at a high level ofhumidity, the interior remains condensa-tion-free and so the risk of contaminationis practically eliminated.

Alarm and error diagnosisBoth acoustic and visual signals informabout any possible fault, which isidentified by the error diagnosis system.

Safety in the event of a powerfailureThe BBD 6220 automatically returns tothe selected incubation conditions assoon as the power supply is restored.

Monitoring and documentationAll operating parameters can be moni-tored, controlled and documented viaan RS 232 digital interface, supplied asstandard. Heraeus Instruments can alsooffer advanced Kelvilog software.

Central monitoringThe BBD 6220 can be supplied with apotential-free contact to connect to acentral monitoring system, if required.

Humidity independent CO2 controlA reliable and stable thermal conductivitydetector acts as a CO2 sensor. TheCO2 level is controlled precisely throughperiodic auto-zero testing and humiditycompensation.

Temperature controlA microprocessor controls the tempera-ture, which is measured via a Pt 100sensor. In terms of internal temperature,time and spatial consistency areimpressive.

THE BBD 6220 – A RANGE OF BENEFITS

The smooth internal surfaces are easy to clean.

CAUTION

Awkward removal of sensors andfittings prior to 180 °C disinfection.

CAUTION

Risk of contamination duringrefitting of sensors and fittings.

CAUTION

Water pans which you have to carrythrough the laboratory to empty.

Detailed examination will reveal thatsuch systems do not eliminate the riskof contamination. To play safe, choosethe consistent quality of incubatorsmade by Heraeus Instruments. Pleasecall us if you want to eliminate risk. Wewill gladly assist you.

BEWARE OF SYSTEMS THATCOMPROMISE SAFETYTHROUGH:

BEWARE OF SYSTEMS THATCOMPROMISE SAFETYTHROUGH:

TECHNICAL DATAORDER NUMBERS TECHNICAL DATA

Type Unit Value/Description

DimensionsExternal dimensions (w/h/d) mm 920 x 855 x 775Internal dimensions (w/h/d) mm 607 x 669 x 585Total volume l 220

ShelvesDimensions (w/d) mm 560 x 500Number of shelves, standard/maximum number 3/12Maximum area load/shelf kg 5Maximum total load/unit kg 30

Weight (without accessories) kg 107

Temperature controlMeasuring and control range °C TR

1) + 5 … 50Temperature deviation (time)2) K ≤ ± 0.1Temperature deviation at 37 °C (spatial) 2) K ≤ ± 0.5Heating up time with auto-start to 37 °C(Ambient temperature 22 °C, empty unit) h ca. 4.5Ambient temperature range °C 18 … 30Recovery time3) min ≤ 3Disinfection method: hot air disinfection °C 180

CO2 controlMeasuring principle thermal conductivity detector with

auto-start and auto-zero functionMeasuring and control range % CO2 0 … 20Operating accuracy % CO2 0.1Recovery time at 5% CO2

3) min ≤ 5 (max. 1.4%/min)

Humidity controlMeasuring principle capacitative humidity controlSetting range % rH 60 … 95Setting accuracy % rH ± 1Recovery time at 95% rH3) min 15

Electrical dataVoltage/frequency V;Hz 230; 50/60 or 120; 50/60Power at 37 °C and 180 °C kW 1.34)

Power consumption at 37 °C/95% rH kWh/h 0.1650 °C/95 % rH0.22disinfection 0.59

1) TR = Ambient temperature 2) DIN 12 880, part 2/11.78 3) at 37 °C, following 30 secs. of door open, to 98% of initial value4) 1 kW at 120 V; 50/60 Hz

ORDER NUMBERS

BBD 6220, Total volume 220 l, 230 V 50047468

BBD 6220,Total volume 220 l, 120 V 50048056

With connection for central monitoring(potential-free contact)



Accessories

Kelvilog software 50047980

On request, the BBD 6220 can be retro-fitted with a six door gas-tight screen.

Australia Kendro Laboratory Products · Lane Cove DC · NSW 2066 · Tel. +61 (02) 9936 1540 · Fax. +61 (02) 9427 9765 · [email protected] Kendro Laboratory Products GmbH · Wien · Tel. +43 (1) 801 40-0 · Fax +43 (1) 801 40 40 · [email protected] Kendro Laboratory Products AB · Albertslund · Tel. +45-43 62 46 47 · Fax +45-43 62 46 41 · [email protected] Kendro Laboratory Products · Courtaboeuf cedex · Tel. +33 (1) 69 18 77 77 · Fax +33 (1) 60 92 00 34 · [email protected] Kendro Laboratory Products GmbH · Hanau · Tel +49 (6181) 35-300 · Fax:+49 (6181) 35-59 44 · [email protected] Kendro Laboratory Products (India) Pvt. Ltd. · New Delhi · Tel. +91 (11) 618 58 40 · Fax +91 (11) 618 53 97 · [email protected] AHSI S.p.A. · Cavenago Brianza · Tel. +39 (02) 95 08 11 · Fax +39 (02) 95 08 12 77 · [email protected] Kendro Spólka z.o.o. · Warszawa · Tel. +48 (22) 663 43 23 · Fax +48 (22) 663 43 25 · [email protected] Heraeus S.A. · Cacem · Tel. +351 (1) 912 08 65 · Fax +351 (1) 912 08 60 · [email protected] China Kendro Laboratory Products Shanghai · Shanghai · Tel. +86 (21) 5490 0218 · Fax +86 (21) 5490 0230 · [email protected] Heraeus S.A. · Madrid · Tel. +34 (91) 358 19 96 · Fax +34 (91) 358 20 67 · [email protected] Kendro Laboratory Products AB · Upplands Väsby · Tel. +46 (8) 59 07 21 90 · Fax +46 (8) 59 03 16 00 · [email protected] Kendro Laboratory Products AG · Zürich · Tel. +41 (1) 454 12 12 · Fax +41 (1) 454 12 99 · [email protected]

Kendro Laboratory Products SA · Carouge-Genève · Tel. +41 (22) 343 21 67 · Fax +41 (22) 342 38 31 · [email protected]./Ireland Kendro Laboratory Products Limited · Bishop’s Stortford · Herts · Tel. +44 (1279) 827700 · Fax +44 (1279) 827750 · [email protected] Kendro Laboratory Products L.P. · Newtown · Tel. +1 (800) 522-7746 · Fax. +1 (203) 270-2166 · [email protected]

All other International Sales in Europe, Middle East, AfricaKendro Laboratory Products International Sales · Hanau · Germany · Tel. +49 (6181) 35-300 · Fax +49 (6181) 35 59 44 · [email protected]

Canada Kendro Laboratory Products International Sales · Newtown · USA · Tel. +1 (203) 270-2080 · Fax. +1 (203) 270-2166 · [email protected] America Kendro Laboratory Products International Sales · Newtown · USA · Tel. +1 (203) 270-2080 · Fax. +1 (203) 270-2166 · [email protected] other International Sales in Asia Pacific

Kendro Laboratory Products International Sales · Norwalk · USA · Tel. +1 (203) 270-2080 · Fax. +1 (203) 270-2166 · [email protected]

Kendro Laboratory Products – a worldwide company formed by the merger of Heraeus Instruments and SorvallInternet http://www.kendro.com . http://www.heraeus-instruments.com

Should your country not be included in the address list, please contact Kendro Laboratory Products International Sales in Germany or USA. Subject to change without notice. product design wolf raimann wiesbaden Printed in Germany 1C 5/99 UN 4t Druckerei

Stand: 10.2005 / 50 046 772 B

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Stand: 10.2005 / 50 046 772 B

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Stand: 10.2005 / 50 046 772 B

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Hot-Air Disinfectable Gassed Incubator

BBD 6220

Read this operating instructions and keep it near the equipment for reference purposes !

Valid: 10.2003 50 079 041

Heraeus

°C

%CO2

% O2

% rH

Operating instructions GB

Kendro Laboratory Products GmbH

Robert-Bosch-Straße 1

D-63505 Langenselbold

++49 / (0)6184 / 90 - 69 40For other tel. nos. refer to An-nex (After-Sales Service, Re-placement Parts, TechnicalEnquiries)++49 / (0)6184 / 90 - 74 74

E-mail [email protected]

Copyright © Kendro Laboratory Products GmbH, 63505 Langenselbold, Germany

No part of this publication may be reproduced or transmitted in any form or by any means, including photo-copying and recording, without the written permission of Kendro Laboratory Products.

Various sections of this instruction manual may be copied only for in-house use by the equipment opera-tor, e.g. to provide employees with instructions on accident prevention measures. These sections areclearly marked in the list of contents.

Heraeus Instruments can accept no liability or responsibility for the marketability or the suitability of this in-struction manual for a certain purpose other than that specified under "Areas of application of equipment".

Kendro Laboratory Products reserves the right to change the content of this instruction manual at any timeand without prior notice.As regards foreign-language translations, the German version of this instruction manual is binding.This edition of the instruction manual applies to the BBD 6220 unit specified on the front page.

Date of publication: 10.2003

Nominal charge

Safety with regard to the protection of persons, the environment and the material under processlargely depends on the conduct of the persons using the equipment.

This instruction manual is important for your safety as well as for the setup, installation, use andmaintenance of the equipment!

To avoid errors and any resulting damage, especially personal injuries, be sure to read this man-ual carefully before using the equipment and follow all instructions.

2/43

50 079 041

1. GENERAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5/7Explanation of icons in the instructions manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Explanation of icons in the short instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6General instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

2. AREAS OF APPLICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3. SAFETY INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

4. SETUP AND INSTALLATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-12Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10Place of installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10Stacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10Clearances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11Supply connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11Gas supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12"Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

5. DESCRIPTION OF THE UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13/14Levelling the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Functional principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Gassing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Humidification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14Internal fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14Six-piece Gas-tight glass screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14Lockable outside door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14"Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

6. OPERATE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15-30Switch panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15Water supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16Mains switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16Temperature protection device (temperature limit controller) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17Control and display panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17-20Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20/21auto-start routine / auto-zero routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22/23Error code enquiry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23/25Switching functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26Switching function: Acoustic alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26Switching function: Humidification ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27Switching function: Manual zero calibration of CO2 measuring system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Switching function: Setpoint lock-in ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29Switching function: Door heater ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

LIST OF CONTENTS PAGE

50 079 041 3/43

7. START-UP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

8. OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32Guidelines for use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32Before putting the unit into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32During operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32Operation interruptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32

9. SHUTDOWN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10. MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34/35Cleaning/Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35Replacing of electrical parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

11. AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

12. TECHNICAL SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37/38

13. MATERIALS USED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

14. GAS CONSUMPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

15. PH-VALUE OF CULTURE MEDIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Basic rules of sound microbiological engineering practice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42/43General rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42Handling of pathogenes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42/43Handling of human- and animal-pathogenic biological agents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

APPENDIXStatement of conformityLog BookExplanation of confirmation of safetyInformation request form regarding maintenance and servicing contractCommunication-protocol of RS 232-device

LIST OF CONTENTS PAGE

4/43

50 079 041

EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL

In chapters of the instruction manual which deal with safety, this icon appears underthe title of the chapter. Displayed on the equipment, this icon denotes that special at-tention must be paid to the information given in the instruction manual or accompany-ing documents.

Marks information in the instruction manual for optimizing use of the equipment

"Water supply" vent/overflow

"Add/drain water" quick release couplingaqua dest

I Unit ON

0 Unit OFF

des start/stop

"Disinfection mode" key-operated switch

"des"Marks information for higher surface temperature in the disinfection mode

Overtemperature protection, temperature limit controller

Selection of switching/interlocking functions

i Error code enquiry / acknowledgment of "acoustic signal"

control "Change switching functions activated" display

auto-start Automatic startup of unit and calibration of measuring systems

auto-zero "auto-zero" calibration active display

des "Disinfection mode" display

Information on proper disposal / recovery of raw materials

1. GENERAL

50 079 041 5/43

EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS

0

ISwitch on unit (operate the main switch)

max.

min.

aqua dest. Check the water level

°C

> + 2 °C

Set the temperature limit controller

Close unit doors

&i Read the operating instructions

desstart/stop

≥ 1 sec

Start "disinfection routine"

auto-start automatical "auto-start"

30 s≥

Open unit doors for at least 30 seconds

° C

%

%

C O.

O.

%

r

H

Set the desired setpoints

≥ 5 s &start / stop

auto-start

"activate" the auto-start

auto-start End of the auto-start, bring in the samples

> 30 s

Take out the samples, open unit doors for at least30 seconds

180 °C

t

Flow chart of disinfection routine

1. GENERAL

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50 079 041

General instructions

To avoid errors and causing damage, especially personal injuries, be sure to read this manualcarefully before using the equipment, and follow all instructions.When setting up and operating the unit, be sure to comply with the instructions contained in thismanual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119,ZH 1/342, ZH 1/343, ZH 1/598).

The unit meets the following safety requirements

DIN 12880 Part 1/11.78

DIN VDE 0700 Part 1 (IEC 335-1; EN 60335-1/11.90)

EN 61010When you have an enquiry, order replacement parts or file a complaint, please state the data onthe nameplate and, if applicable, the fault code.

Equipment log book

We advise you to keep an equipment log book.

Keep a record of inspections and testing, calibration work and any major work carried out on theunit - such as maintenance, agents loaded, etc. - in this log book (refer to APPENDIX).

Operating instructions

The operator (employer) is expected to provide anyone who works on or with the equipment withwritten instructions, based on this instruction manual, for the tasks to be performed. These in-structions should be easy to understand and in the language of the persons operating the equip-ment.

This also applies to disinfecting and cleaning the unit (also refer to Chapter 10: Maintenance).

1. GENERAL

50 079 041 7/43

The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue culturesand can be used to simulate the physiological environmental conditions of cells.

The unit is generally suitable for setup and operation in the following areas:

In laboratories for microbiological and biotechnological work.

Medical and microbiological laboratories to DIN 58956.

Central laboratories in clinics and hospitals.

Laboratories operating at safety levels L1, L2 and L3.

Only organisms which comply with the requirements for safety levels L1, L2 and L3 may behandled in the unit.

The unit must be set up and operated in accordance with all applicable regulations in yourcountry.(FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

It is not permitted to handle gases or agents whose vapours are combustible or can form ahazardous, potentially explosive atmosphere when mixed with air.Be sure to comply with the applicable regulations in your country.(FRG: ZH 1/10)

2. AREAS OF APPLICATION

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50 079 041

In the case of biological incubators, biological safety with regard to the protection ofpersons, its surroundings and the load is heavily dependent on the observance of theapplicable regulations by the persons using the equipment.

Even then, however, the possibility of hazards, especially health hazards, arising cannot beruled out.

The residual risk depends on the work performed in each individual case.

To avoid errors and causing damage, especially personal injuries, be sure to carefully readthis manual before putting the equipment into operation, and follow all instructions.

Safe and reliable operation of the unit can only be guaranteed if the necessary inspections,maintenance and repair work are carried out by Heraeus Service Department personnel orby personnel authorized by our company.

Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressureset to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons(FRG: ZH 1/119).

The place of installation must be thoroughly ventilated in order to expel the gases escapingaround the pressure relief valve to the outside.

To maintain the temperature protection function, the functioning of the overtemperatureprotection device, temperatur limit controller must be checked at reasonable intervals.

Only original replacement parts authorized by the manufacturer are to be used.

Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEER-ING PRACTICE".

3. SAFETY INSTRUCTIONS

50 079 041 9/43

When setting up, installing and operating the unit, be sure to comply with all applicable regulationsin your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

Transport

Handle the unit with care during transport. Do not lift up the unit by its door.Refer to TECHNICAL SPECIFICATIONS for weights and dimensions.

Place of installation

The place of installation must be dry and draught-free.

The unit should not be placed or operated in recesses which cannot be ventilated.

To avoid or at least minimize deviations from the technical specifications, the tempera-ture at the place of installation must be in the range +18°C to +30°C.

Set up the unit on a solid, non-flammable surface (laboratory tables, subframes).

Make sure that the unit is in a stable and perfectly upright position.

Do not cover or obstruct the fresh air or exhaust vents in the unit housing(please observe the minimum clearances - see page 11).

CO2 are admitted into the gassed incubator. CO2 is a health risk hazard. The place of installa-tion must therefore be well-ventilated.

Gases discharged from the pressure relief valve must be safely expelled outside by means of theventilation systems.

It may be necessary to take special measures in order to ensure proper ventilation when several units are installed in the same room.

For information on gas flow during operation, refer to the APPENDIX.

Stacking

The unit is not suitable for stacking when operated in the manner intended.

4. SETUP AND INSTALLATION

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50 079 041

Clearances

When setting up the unit, the minimum clearances between the unit and adjacent surfaces andother units must be maintained.

Larger clearances are recommended to facilitate accessibility for installation and the supply con-nections.

Fig. 1: Minimum clearances in mm

Supply connections

Fig. 2: Supply connections (rear panel of unit)

Mains connection

Prior to connecting the unit to the mains, make sure that the power supply ratings match thosestated on the nameplate.

Your unit is equipped with a power supply cable with a grounding contact-type plug.

Fuse protection required:Use a type G 16 circuit-break switch or a type T 16 A fusible cutout.

Refer to the Chapter titled TECHNICAL SPECIFICATIONS for unit power consumption.


50 079 041 11/43

RS 232 interface

Potential-free contact

C0 gas connection2

Mains socket

0 /N (option)

2 2 gas connection

Label:supply connection

150100

300 100

Gas supply connection

The gas connection are located on the back of the unit.

The gas must be supplied to each unit via a pressure reducer with the inlet pressure setto between 0.8 and max. 1 bar.This setting must not be changed for safety reasons (FRG: ZH 1/119).

The gases must have at least 99.5% purity.If several units are placed in the same room, special ventilation measures are required.

CO2 gassingConnect the gas cylinder to the gas nozzle on the unit.O2 / N2 gassing (optional)If you intend to run the unit with an oxygen content above 21%, connect an oxygen cylin-der. If you intend to run the unit with an oxygen content less than 21%, connect a nitrogen cylin-der. To deactivate the oxygen control, set the nominal value to 21.0% (average oxygen contentof ambient atmosphere).

RS 232 interface

Serial interface for computer-aided acquisition and documentation of critical operating parameters(temperature, CO2, relative humidity, error codes, etc.). The RS 232 interface can connect a singleunit in combination with the "Kelvilog" software package, and can be expanded to connect 31units with the "Netcontrol" hard- and software package.

"Potential - free contact" connection

The "Potential-free contact" (1 changeover contact) is rated for the following circuits:

Circuit Voltage Fuse to be installed by customerMains-operated circuits max. 250 V AC max. 6 ASELV / SELV - E - circuits(cf. VDE 0100 Part 410)

25/50 V AC60/120 V DC

max. 2/1 Amax. 1/0.5 A

Fig. 3 : Application example of "Potential - free contact" connection

3 1

2

XView X

PE 3

1 2

L F1x

T2A

H1x KxS1xKx

H2x Kx A1

A2N

1s


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50 079 041

Levelling the shelves

The shelves can be aligned with the aid of a spirit level with height-adjustable feet.

Functional principle

The atmosphere in the chamber can be regulated in relation to its temperature, CO2 concentra-tion, O2 concentration (optional) and relative humidity.

Heating system

The temperature inside the unit can be regulated in the range of +7°C to +50°C, but must be atleast +5°C (approx. +7°C for the O2 version) above the ambient temperature of the unit.Condensation on the glass door is largely prevented by heating the unit door. If the unit door is leftopen for a long period of time, however, condensation cannot be ruled out.

The unit door heater can be switched off as required (refer to Chapter headed OPERATION).This enables the unit to be operated at high ambient temperatures or when the operating tem-perature is only supposed to exceed the ambient temperature by approx. +4°C.

Example:Ambient temperature Operating temp. Heating of unit door

+25°C +37°C ON+24°C +28°C OFF

+32°C to +33°C +37°C OFF

Gassing

Connection to the gas supplies (CO 2,, O2 or rather N2) are located on the rear panel of the incuba-tor.

The CO2 content of the atmosphere in the chamber can be regulated in the range 0% to20%.

The O2 concentration inside the unit can be regulated in the range 3% to 90% O2 by admit-ting N2 (< 21% O2) or O2 (> 21% O2).

The sum of the nominal values for CO2 + O2 must not exceed 90%. Example: 10% CO2 + 80% O2 = 90% (possible) 20% CO2 + 80% O2 = 100% (impossible)

Before entering the chamber, all gases pass through a filter where particles larger than 0.3 µm areretained. Filter efficiency is 99.998%.A fan integrated in the rear of the interior wall ensures that the gases and the incubator atmos-phere are thoroughly mixed.The sensors for CO2, O2 (option) and relative humidity are also located on the rear panel of the in-terior wall. These sensors measure the parameters inside the unit and transmit corresponding sig-nals to the closed-loop controller.A pressure compensation vent on the rear panel of unit avoids undesirable pressure build-up in-side the unit during the admission of gases.The place of installation must be thoroughly ventilated in order to expel the gases escapingaround pressure compensation vent.

5. DESCRIPTION OF THE UNIT

50 079 041 13/43

Humidification

The atmosphere in the chamber is humidified through the admission of steam generated in a aktivhumifier. Relative humidity (rH) can be regulated between 60% and 95%.

To ensure reliable operation of the steam generator, only fill the reservoir with fully desali-nated or distilled water! The capacity of the reservoir is approx. 4.7 ltr (difference between min. and max. levels:2.2 ltr).

Door switch

The heating system, gas supply and humidifier are switched off when the unit door is opened.

The unit door can only be closed when all glass doors are sealed properly. If the unit door isnot properly closed while the unit is in operation, a visual alarm is given (all LEDs flashing). Ifthe door is open for longer than 10 minutes, an acoustic alarm sounds when this monitoringfunction is activated by way of function level 1 (refer to Page 26).

Internal fittings

Pull-out shelves, with support brackets to prevent tilting, are arranged inside the units. The sup-port can be offset in the carrier racks at intervals of 46 mm.

Six-piece gas-tight glass screen

By virtue of the small vent cross-section, unnecessary cooling and discharge of the atmosphere inthe chamber is largely avoided when a glass door is opened of the six-piece gas-tight glassscreen.

Lockable outside door

The outside door is lockable.

Disinfection routine

This routine is for disinfecting the interior of the unit. In this process, the unit is heated to 180°Cand kept at the disinfection temperature for approx. 3 hours. The unit is then automatically cooleddown again and the nominal values for temperature, gases and relative humidity are reset. Theentire procedure lasts approx. 13 hours, after which the unit is ready for operation again.

"Potential - free contact" connection

Connection for the customer's own signalling system, e.g. telephone system, building servicesmanagement systems.If the overtemperature-protection device trips or an error is detected by the diagnosis system, afault message is sent to the customer's own signalling system.

5. DESCRIPTION OF THE UNIT

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Switch panel

(A) "Control and display panel" (refer to Fig. 7)

(1) Indicator lamp "Fault, overtemp. protection"

(2) Indicator lamp "Disinfection mode"

(3) Indicator lamp "Master Switch"

(4) Vent/overflow "Water supply"

(5) Level gauge "Water supply"

(6) Setting knob "Temperature protection device"

(7) Press button "Disinfection mode"

(8) Master switch

(9) Quick-release coupling "Add/drain water"

Fig. 4: Switch panel

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max

min

5.0

7.0

37.0autozero

des

95.0

a q u a d e s t

°C

%CO2

auto-start

control

i

0

I

d e s s t a r t / s t o p

( 1 )

( 2 )

( 3 )

( 4 )

(5)

( 6 )

( 7 )

( 9 )

(A)

( 8 )

7.095.0 %rH

% O2

Heraeus

Water supply

Level gauge (5)indicates the water level.

The water level should be in the range between "min and max". This range is equivalent toa volume of 2.2 ltr. If the water supply falls below the "min." level, a reserve of 0.5 ltr. is stillavailable.

To guarantee proper operation and avoid calcium deposits, only use distilled or fully desali-nated water.

Always rinse the filling canister supplied with the unit with distilled or fully desalinated waterprior to use.

Filling/draining the reservoir

Fill the filling canister with distilled or fully desalinated water and re-attach the screw cap.Attach the canister mount to the outside of the housing at the top left and put the filledcanister into the mount. Join the nozzle of the canister to the reservoir using the quick-release coupling (9). The water flows from the canister into the water reservoir. If waterdoes not flow out of the canister, it may be necessary to press the bellows integrated inthe hose and expel the air from the hose.

To release the quick-release coupling, depress the metal lug and disconnect.

Caution:If the water reservoir is overfilled, the excess water is discharged via the "vent/overflow"outlet (4).

As the outlet not only functions as an overflow but also as a vent, it must be unsealed whilethe unit is in operation.

To drain the empty filling canister, place it on the floor and connect it using the quick-release coupling (9). Take care that the filling canister does not overflow. Disconnect thequick-release coupling if necessary.

Always drain the water reservoir before transporting the unit.

Mains switch

0

I

Unit OFF

Unit ON (the green indicator lamp (3) indicates that unit is ready for operation).

Fig. 5: Mains switch (8)

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Temperature protection device (temperature limit controller)

A temperature protection device is built into the unit and acts as a temperature limitcontroller. It conforms to Thermal Safety Class 3.1 to DIN 12880 Part 1/11.78. In a functional state, the temperature limit controller assumes the control function if theset temperature is exceeded.The red indicator lamp (1) "Fault" lights up as soon as the temperature limit controllerresponds.

Fig. 6: Temperaturelimit controller (6)

The cutout temperature of the temperature limit controller can be adjusted in the range+20°C to +55°C using a coin or screwdriver.

Have your temperature protection device tested for proper operation at reasonable intervals.This applies in particular before prolonged work processes.

Operational test:Condition: Nominal temperature (controller) reached/constant.To run this test, turn the dial on the Temperature Limit Controller so that it shows approx.+5°C less than the temperature indicated on the temperature controller.If the Temperature Limit Controller responds and the red indicator lamp (1) "Fault" comeson, the Temperature Limit Controller is operating properly.

Now set the Temperature Limit Controller to the required value depending on the max. cutout tem-perature.

If the Temperature Limit Controller is set to the upper temperature limit, it assumes thefunction of unit protection (protection of unit and surroundings).

If the Temperature Limit Controller is set to approx. +2°C above the nominal temperatureset at the temperature controller, it assumes the function of load protection (protection ofunit, its environment and loaded material).

The load protection function is only effective above room temperature.

If "Fault" is displayed during operation:Check the settings on the Temperature Limit Controller and the controller, and correct themif necessary. If the fault cannot be rectified, contact our service department.

Control and display panel (A)

Microprocessor-controlled controller with digital temperature, CO2, O2 (optional) and relative hu-midity displays.The controller has the following functions:

Temperature "set, display and control"CO2 concentration "set, display and control"O2 concentration "set, display and control" (OPTIONAL)Relative humidity "set, display and control"Error code "scan"auto-start function "activate"Switching functions- Acoustic alarm "switch ON/OFF"- Humidification "switch ON/OFF"- Manual calibration of CO2 measuring system "execute"- Setpoint lock-in "select ON/OFF- Door heater "switch ON/OFF"

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Fig. 7: Control and display panel (A)

(1) Indicator lamp "HEATING" (11) Indicator lamp "Disinfection mode"

(2) Display "Temperature" (12) Key "Temperature setpoint"

(3) Indicator lamp "CO2 GASSING" (13) Indicator lamp "auto-zero"

(4) Display "CO2 concentration" (14) Key "CO2 setpoint"

(5) Indicator lamp "O2/N2 GASSING" (optional) (15) Key "O2 setpoint" (optional)

(6) Display "O2 concentration" (optional) (16) Key "Relative humidity setpoint" (7) Indicator lamp "HUMIDIFICATION" (17) Indicator lamp "auto-start" (8) Display "Relative humidity" (18) Key "Error code inquiry" / Start

calibration routines

(9) Key "Increase reading" (19) Indicator lamp "Control"

(10) Key "Decrease reading" (20) Key "Switching functions"

6. OPERATE

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-

+

5.0

37.0

95.0

(2)

(3)

(4)

(5)

(6)

(7)

(1)

(17)

(16)

(15)

(14)

(12)

(8)

(11)

(10)

(9)

(20)

(13)

(18)

(19)

°C

%CO2

%

%

O

rH

iauto-start

control

autozero

des

95.0

In its as-delivered condition, the unit is preset to the following values:Temperature: 37.0°CCO2 concentration: 0.0%O2 concentration: 21.0% (optional)Relative humidity: 60.0%, humidity control activated via function level 2

Setting the temperature setpoint:

Instruction Entry / Keys / Remarks Display / Remarks / StatusSwitch on unit(master switch = " I ")

All display elements come on forapprox. 15 seconds.- Auto-test of controller -

Example:

Display temperature setpoint °C press Actual setpoint is displayed anddecimal point flashes

Select temperature setpoint press&°C°C Setpoint increases

press&°C°C Setpoint decreases

Adopt NEW temperature set-point °C release Temperature inside unit is dis-

played

Setting the CO2 setpoint:

Instruction Entry / Keys / Remarks Display / Remarks / StatusDisplay CO2 setpoint %CO2 press Actual setpoint is displayed and

decimal point flashesSelect CO2 setpoint %CO2 drücken& Setpoint increases

%CO2 drücken& Setpoint decreases

Adopt NEW CO2 setpoint %CO2 release CO2 concentration inside unit isdisplayed

Setting the O2 setpoint (optional):

Instruction Entry / Keys / Remarks Display / Remarks / StatusDisplay O2 setpoint Actual setpoint is displayed, deci-

mal point flashesSelect O2 setpoint Setpoint increases

Setpoint decreases

Adopt NEW O2 setpoint O2 concentration inside unit isdisplayed

The control is deactivated when the unit is set for 21% O2 since this corresponds to the O 2 content of air.

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Setting the relative humidity setpoint:

Instruction Entry / Keys / Remarks Display / Remarks / StatusDisplay the relative humiditysetpoint

%rH press Actual setpoint is displayed anddecimal point flashes

Set the relative humidity set-point

%rH press& Setpoint increases

%rH press& Setpoint decreases

Adopt NEW relative humiditysetpoint

%rH release Relative humidity concentrationinside the unit is displayed

Disinfection routine

The entire interior of the unit together with all sensors can be disinfected with hot air at 180°C. Thedisinfection routine lasts approx. 13 hours.

Start disinfection routine:

Instruction Entry / Keys / Remarks Display / Remarks / StatusOpen the unit door for at least30 seconds

All displays flash

Remove samples and vesselsfrom the interior of the unitActivate the disinfection rou-tine (turn key in direction ofarrow)

desstart/stop

Indicator Lamp (page 18 (11))flashes and indicator lamp (page 15(2)) comes on.

Press for approx. 1 second Close the door Disinfection routine runs automati-

cally.Temperature Limit Controller is dis-abled.CO2 display indicates the phases ofthe disinfection routine."auto-start" routine begins on com-pletion of the disinfection routine .

!"des"

In the disinfektion mode (appr. 13 h) occur slightly higher temperatures on the outer sur-face, the door area and the internal door grip. When LED "des" is flashing, proceed withparticular caution.Avoid touching the door areas during the disinfection routine.

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Flow chart of disinfection routine:

Status display on CO2 display:

Heat:

The chamber is heated to 180°C; the disinfection temperature can be read off the temperaturedisplay.

Disinfect:

When the disinfection temperature of 180°C is reached, a disinfection phase with a duration ofapprox. 3 hours is started. If the door is opened during the period and the temperature dropsbelow 180°C, the disinfection routine restarts automatically.

Cool:

On completion of the disinfection phase, the unit cools down to the originally set nominal temperature.

The yellow indicator lamp "des" as soon as the unit reaches the set operating temperature(e.g. 37.0°C) is reached. The sensors are automatically calibrated (auto-start) and the unitis readjusted to the set gas / rel. humidity setpoints. The disinfection routine is completed.

If a temperature of 200°C is exceeded during the disinfection routine, the latter is cancelledand all poles of the heating system are disconnected. Error message 501 is displayed.

Cancelling the disinfection routine:

Instruction Entry / Keys / Remarks Display / Remarks / StatusCancel disinfection des

start/stop

The CO2 display indicates the cool-ing phase of the disinfection routine.

Press for approx. 1 second

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StartTime

37°C

180°C

Temp.

End

Heating phase Disinfectionphase Cooling phase

auto-start routine / auto-zero routine

An auto-start routine / auto-zero routine will have to be run when putting the unit into operation or ifthe temperature setpoint is altered by more than 1°C.

To ensure that there is only air, i.e. no CO2 in the chamber before the auto-start routine is acti-vated, ventilate the chamber by opening all doors.The auto-start can only be activated if the doors are open for at least 30 s.

The indicator lamp "auto-start" (page 18 (17)) indicates when the auto-start routine is running.Calibration can take up to 5 hours, especially if the unit is cold.

If the unit door is opened during the auto-start, the auto-start routine / auto-zero routine restartsautomatically after the unit door is closed.

On resumption of the power supply after a power failure, the auto-start routine / auto-zero routineis also restarted.

Instruction Entry / Keys / Remarks Display / Remarks / StatusOpen unit doors for at least30 seconds

All display segments flash

Adjust or check the set-points

Refer to pages 19

Activate auto-start & Indicator lamp (page 18 (17)) "auto-start" flashes

Press for approx. 5 secondsClose all unit doors Temperature reads "Actual value"

CO2 reads " 0.0"O2 reads "21.0" (optional)rel. humidity reads "Actual value"

The unit is set to the temperature and relative humidity setpoints. When temperature and relative hu-midity setpoints are achieved consistently, the CO2 measuring system is automatically calibrated andthe O2 sensor (optional) is calibrated for 21%.

Indicator lamp (page 18 (17)) "auto-start" goes out.

The auto-zero routine determines the reference value and O2 sensor (optional) is calibrated.

Gas is admitted until the CO2 /O2 setpoints are reached.

The auto-zero routine compensates for zero drift of the CO2 measuring cell. The auto-zero routineruns automatically every 6 hours. When the indicator lamp (page 18 (13)) "auto-zero" is flashing,the auto-zero routine is in progress. If the door is opened or closed during the auto-zero routine,auto-zero calibration is restarted.

If the auto-start has not been completed after 24 hours at the latest (page 18, indicator lamp (17)"auto-start" is still flashing), the auto-start will have to be cancelled manually (refer to page 23)and repeat.

6. OPERATE

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Instruction Entry / Keys / Remarks Display / Remarks / StatusCancel auto-start routine & Indicator lamp (page 18 (17)) "auto-

start" goes out and auto-zero cali-bration routine starts Press for approx. 5 seconds

Indicator lamp (page 18 (13)) "auto-zero" flashes

Cancel auto-zero routine & Indicator lamp (page 18 (13)) "auto-zero" goes out

Press for approx. 5 secondsUnit displays the current actual values

If a repeat auto-start / auto-zero routine is unsuccessful, contact the service department of Kendro Laboratory Products.

Error code request

The unit is equipped with an error diagnosis system.

This diagnosis system lets you detect and evaluate a fault during operation by means of specificcodes.

If an error occurs within a control loop, the relevant display flashes to indicate this.

Press (page 18 (18)) to display the error detected by the diagnosis system and to acknowl-edge the alarm.

When the error is cleared, the error message is automatically closed and the display stops flashing.Exception: Error 502

Instruction Entry / Keys / Remarks Display / Remarks / StatusScan error code /Acknowledge acoustic alarm

i Hold down Error code is displayed/acousticalarm is acknowledgedDisplay " - - - ":No error detected

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50 079 041 23/43

i

ERROR CODES

CODE CAUSE Error condition POSSIBLE REMEDY

88auto-start

Error during auto-start Measuring cell outside cali-bration range

Repeat the auto-start routine (refer topages 22)

99≥ 10 min

Unit doors are open Doors are open for morethan 10 minutes

Close the unit doors(refer to page 14) or just acknowledgeacoustic alarm

100Temperature belowsetpoint

Actual value < setpoint-0.5°C

Check the settings on the controllerand Temperature Limit Controller (re-fer to pages 17 and 19)

101Temperature abovesetpoint

Actual value > setpoint+1°C

Ambient temperature could be toohigh: check the circuit status of thedoor heater (refer to pages 15 and 30)

104 °C Temperature sensorfaulty

Sensor broken/short-circuited

Contact the service department ofHeraeus Instruments

200 CO2

CO2 below setpoint Actual value < setpoint -1% Check the gas supply for - cylinder content - inlet pressure - supply line and connection to unitRefer to page 12

201 CO2CO2 above setpoint Actual value > setpoint

+1%Check the gas supply for - inlet pressureRefer to page 12

204 CO2CO2 measuring cellfaulty

Sensor broken/short-circuited


300

N 2N2 below setpoint Actual O2 value <

setpoint -2%Gassing with N2

Check the gas supply for - gas cylinder content- inlet pressure- supply line and connection to unit Refer to page 13/14

301 N 2

N2 above setpoint Actual O2 value > setpoint +2%Begasung mit N2

Check the gas supply for- inlet pressureRefer to page 13/14

302

O2 O2 above setpoint Actual O2 value < setpoint -2%Gassing with O2

Check the gas supply for- gas cylinder content- inlet pressure- supply line and connection to unit Refer to page 13/14

303 O2 O2 above setpoint Actual O2 value >

setpoint +2%Gassing with O2

Check the gas supply for- inlet pressureRefer to page 13/14

304 2NO2

O2 sensor faulty Contact service

400 %Relative humidity be-low setpoint

Actual value < setpoint -5% Check the water reservoir Contact the service department ofHeraeus Instruments if necessary

404 % Relative humidity sen-sor faulty

Sensor faulty/short-circuited


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405Evaporator faulty 95°C > Actual value

> 620°CContact the service department ofHeraeus Instruments

500des "des" temperature

below setpointActual value < 180°C -10°C Repeat the disinfection process Con-

tact the service department of Her-aeus Instruments if necessary

501 des"des" temperatureabove setpoint

Actual value > 180°C+10°C


502 des

Error during disinfec-tion procedure

Power failure > 1 sec Press the des buton (Fig.4, (7)) to re-set the error and repeat the disinfec-tion procedure; check voltage supplyfor power failure

A malfunction of the unit is not the only explanation for error codes 101, 201. They can also bedisplayed after a setpoint is decreased.

Example:

When operating the unit with the setpoint entered for 10% CO2 :If the CO2 setpoint is decreased to 5% CO 2, the unit cannot reach this lower CO2 concentrationquickly enough due to its tightness. Error 201 is displayed.

The designated error messages are displayed when:Setpoint < actual value.

Setpointtemperatur < ambient temperature + 5°C

To avoid this, open the doors for a short time. This also applies to the reduced temperature.

If these measures are unsuccessful or if errors with undefined codes occur, please notify ourservice department (refer to Appendix).

When requesting our service, please state the scanned error code.

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Switching functions

Press - Key (page 18 (17)) to select the individual switching functions as required. The indicator lamp "control" (page 18 (16)) indicates when one of the switching functions specifiedbelow is selected:

Acoustic alarm ON/OFF

Humidification ON/OFF

Manual zero calibration of CO2 measuring system

Setpoint lock-in ON/OFF

Door heater ON/OFF

Switching function: acoustic alarm ON/OFF

Factory setting: acoustic alarm switched on.

Instruction Entry / Keys / Remarks Display / Remarks / StatusSelect function level 1 Press Indicator lamp "control" flashes

and current function level is indi-cated by a flashing display

+ or Press °C

Circuit status display andfactory setting:Acoustic alarm ON

Release and press again °C

CO2

Change circuit status:acoustic alarm OFF Press °C

CO2

Change circuit status:acoustic alarm ON + Press °C

CO2

Exit function level Release Actual temperature, CO2, O2 andrel. humidity values are displayedagain after 1 minute

Press any setpoint key Actual temperature, CO2, O2 andrel. humidity values are displayedagain immediately

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50 079 041

Switching function: Humidification ON/OFF

Factory setting: Humidification switched on (set to 60 % rel. humidity).

Instruction Entry / Keys / Remarks Display / Remarks / StatusSelect function level 2 Hold down Indicator lamp "control" flashes

and current function level is indi-cated by the flashing display

+ or Press °C

Display circuit status,factory setting:Humidification ON

Release and pressagain °C

CO2

Change circuit status:Humidification OFF Press °C

CO2

Change circuit status:Humidification ON + Press °C

CO2

Exit function levelPress

Actual temperature, CO2, O2 andrel. humidity values are displayedagain after 1 minute


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Switching function: Manual zero calibration of CO2 measuring system

Recommendation: Check the CO2 concentration, especially during long-term tests.If there is a large deviation (± 0.5 %) between the displayed actual value for CO2 concentrationand the nominal value, the measuring system can be recalibrated during operation.

Example: Display: 5.0 % CO2Comparative measurement: 4.2 % CO2

Instruction Entry / Keys / Remarks Display / Remarks / StatusSelect function level 3 Hold down Indicator lamp "control" flashes and

the current function level is indi-cated on the flashing display

+ or Press °C

Display zero point Release and pressagain °C

CO2

Enter determined CO2 con-centration + or Press °C

CO2

Start calibrationprocess/exit function level Release

i Press"CAL" is briefly displayed.The corrected actual value is thendisplayed

6. OPERATE

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Switching function: Setpoint lock-in ON/OFF

To prevent unintentional or unauthorized modification of the setpoints for temperature, CO2, O2and rel. humidity, the setpoints can be "locked in".The setpoints cannot be changed until the interlock has be cancelled again.Factory setting: setpoints unlocked.


current function level is indicated byflashing display

+ or Press °C

Display circuit status,factory setting:setpoints released

Release and hold downagain °C

CO2

Change circuit status:locked setpoints Press °C

CO2

Change circuit status:Release setpoints + Press °C

CO2

Exit function levelRelease



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Switching function: Door heater ON/OFF

To adapt the unit to the ambient temperature in the place of installation, the door heater can beswitched on or off (see page 13 for example).

Condensation can occur inside the unit, especially on the glass door or glass screen, when thedoor heater is switched off.

Factory setting: door heater switched on.


the current function level is indi-cated by the flashing display

+ or Press °C

Display circuit status,factory setting:Door heater ON

Release and hold downagain °C

CO2

Change circuit status:Door heater OFF Press °C

CO2

Change circuit status:Door heater ON + Press °C

CO2

Exit function levelRelease



6. OPERATE

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50 079 041

The items specified in brackets refer to Fig. 4 on page 15.

Instruction Entry / Keys / Remarks Display / Remarks / StatusOpen unit doorsRemove transit restraints andaccessories from inside the unitCarry out basic cleaningWater reservoir Fill unit with water -- observe max. level, use

destilled or fully desalinatedwater

Switch on unit Mains switch (8) = " I " All display elements come onfor approx. 15 seconds- auto-test of controller -O2 version:The O2 measuring system isstarted up automatically oncompletion of the auto test.Duration: approx. 5 min.O2 display reads "run"

Adjust setpoints Control and display panel (A) Refer to page 17-20Set temperature protection de-vice

Adjust Temperature Limit Control-ler (6)

Refer to page 17

Select switching function(s) asrequired

On control and display panel (refer to page 18 and 26 to 30)

Activate auto-start+ & Press

Indicator lamp "auto-start"flashes

Hold down for approx. 5 secondsClose all unit doors Temperature displays "actual value"

CO2 displays "0.0",O2 displays "21.0",rel. humidity displays "actual value"

auto-start routine runs automatically. Unit is set to entered temperature and relative humidity set

points.

Relative humidity is built up.

When a constant temperature and relative humidity arereached, the CO2 and O2 measuring system is calibratedautomatically.

Indicator lamp "auto-start" goes out.

"auto-zero" routine for determining reference values is exe-cuted.

Gas is admitted until the entered CO2 / O2 setpoints arereached.

7. START-UP

50 079 041 31/43

Operating control

The microbiological safety of the unit is largely dependent on the proper conduct of the personsusing the unit. Refer to the Appendix "Basic rules of sound microbiological engineering practice".

Be sure to comply with the instructions contained in this manual as well as all applicable regula-tions and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598).

Before putting the unit into operation

Put on all safety garments necessary to protect hands, face and body; remove jewelry.

Disinfect and clean the chamber and fittings regularly. Only use lint-free materials to wipesurfaces clean.

Do not use explosive disinfectants. When using alcoholic disinfectants, be sure they complywith all applicable regulations of your country (FRG: ZH 1/598).

During operation

Correct loading determines the temperature conditions in the chamber. If large objects orauxiliaries are placed inside the chamber, this can impair the temperature distribution in theunit.

When loading the unit, do not arrange articles too close to one another on the shelves(use only approx. 70 % of surface area) in order to avoid impairing air circulation and en-sure constant heat flow.

Do not impair temperature distribution. This is mainly caused by the following:

Covering the air vents.

Voluminous objects or apparatus.

Equipment releasing large amounts of heat.

Do not change the settings of the temperature protection devices as this will render loadprotection ineffective.

Operating interruptions

If operations are interrupted, make sure that no contamination hazards can occur.Remove, disinfect and clean any objects or auxiliaries put into the unit.

Disinfect and clean the chamber.

Disinfect and remove any residues.

Disinfect the unit with hot air (disinfection routine).

8. OPERATION

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50 079 041

!

The unit may only be shut down by qualified staff trained to operate this equipment.The unit must not present any risk of contamination after shutdown.

Shut off the gas supply.

Remove, disinfect and clean any objects or auxiliaries put into the unit.

Disinfect and clean the chamber. Leave the unit doors open until the chamber is dry.

Disinfect the unit with hot air (disinfection routine).

Set the mains switch to "0" and disconnect the power plug.

9. SHUTDOWN

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Cleaning / Disinfection

Be sure to disinfect the unit in compliance with the applicable regulations in your country(FRG: ZH 1/598).

The chamber and fittings are decontaminated through hot air disinfection at 180°C.The sensors can be left inside the unit during the disinfection process.Do not spray the O2 / rel. humidity sensors (porous sintered material) with disinfectant.

We recommend the following disinfectant: "Barrycidal 36".Provided that it is used correctly, "Barrycidal 36" is a highly effective disinfectant.

Order Disinfection:Remove samples, cultures, etc., from the unit.

Switch off the unit.

Remove shelves and carrier racks.

Remove safty device from the rear panel of the inner vessel with tools ( e.g. screw driver)and detach them by lifting it up.

Clean inner housing, shelves and carrier racks using a warm, skin-sensitive detergent ("Pril") and allow them to dry completely.Do not wet sensors ( 4, 6, 8) with liquids and disinfectants.

1.) Lead-through and pressure compensation vent2.) Steam outlet: Do not fill unit with any liquids -

only clean the surface3.) Recirculated air blower4.) O2 sensor (optional)5.) Pt 100 temperature sensors 6.) Relative humidity sensor. 7.) Tubular radiator for disinfection8.) CO2 measuring cell

Fig. 8: Rear wall of inner vessel

Also clean glass door and seal with detergent and allow them to dry completely.

Then spray surfaces and seals with "Barrycidal 36" and allow it to reactin accordance with the manufacturer´s instructions for use.

Then rinse surfaces with distilled water several times until all traces of disinfectant have been removed. Collect the washings.

The surfaces of the unit can also be re-rinsed or sprayed with a 70% by vol. alcohol solution (analytically pure isopropanol) if required.

Attention: Be sure to comply with the safety instructions and applicable regulations in your coun-try!

Alcohols or solutions with an alcohol content of greater than 10% can ignite or form explosivegaseous mixtures when they come into contact with air and should therefore only be used inwell- ventilated places. Do not expose to naked flame.Equipment and component parts cleaned using alcohol or alcoholic solutions must not be ex-posed to naked flames or other possible hazard sources. Wait until dry before use!

Install the parts in the reverse order of removal as appropriate.

10. MAINTENANCE

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50 079 041

1

23

5

6

78

4

!

Disinfect the unit with hot air at 180°C (disinfection routine).

After disinfection, put the unit back into operation as described and always check thatit is safe to operate prior to loading.

Testing

The serviceability and safety of the unit are only guaranteed if the necessary testing, main-tenance and repair work is carried out by the Heraeus service department or by staffauthorized by us.

The unit should be checked for safety, leakproofing and serviceability at least once a year.

Equipment log book

We recommend you keep an equipment log book (refer to APPENDIX).

Keep a record of inspection and tests, calibration work and any major work carried out on the unit(e.g. maintenance work, agents loaded, etc.) in this log book.

Replacement of electrical parts

Work on electrical components of the unit may only be carried out by Heraeus service de-partment personnel and when the unit is in a deenergized state (disconnected from themains supply).

Only use original replacement parts approved by Heraeus Instruments.

The sensors may only be replaced by authorized personnel of the operator.

10. MAINTENANCE

50 079 041 35/43

The safety and serviceability of the equipment are only guaranteed if the approvedoriginal replacement parts specified below are used.

Use of other parts presents unknown risks and is not approved under anycircumstances.

Replacement part Type Order No.Instruction manual 50 047 879Hose set for connecting unit to gas supply 26 139 129Unit foot, upper part 50 044 921Foot, height-adjustable, lower part 50 029 5872 support brackets 50 011 392Shelf 50 011 401Shelf, divided 50 011 380Glass door 50 025 445Protective panel 50 035 268Gasket for iner-casing front 03 650 374Glass door for gas-tight glass screen, left 50 030 020Glass door for gas-tight glass screen with feed-trough, left

50 030 021

Glass door for gas-tight glass screen, right 50 030 022Seal for glass door for gas-tight glass screen 50 041 536Filler canister 50 029 126Equipment fuse T 6,3 A (slow-blow)(2 pcs.) 03 002 641Sealing plug for feed-through (without hole) 03 669 008Sealing plug for feed-through (with hole) 50 029 827Drain plug for protective panel 2 pcs. 26 136 273Subframe, 300 mm high 50 031 348Subframe, 780 mm high 50 029 597Pressure reducer for CO2 03 429 937Pressure reducer for N2 03 429 942Pressure reducer for O2 03 429 943

11. AUTHORIZED REPLACEMENT PARTS AND ACCESSORIES

36/43

50 079 041

TECHNICAL SPECIFICATIONSUNITS: VALUE:

MECHANICAL:Outer dimensions (W x H x D) mm 920 x 855 x 775Inner dimensions (W x H x D) mm 607 x 669 x 585Chamber volume l approx. 220Divided Shelves (W x D)Quantity included in scope of supplyMax. quantityMax. surface loadMax. gross load of unit

mmpcspcskgkg

260 x 50031210 per shelf30

Weight of unit excluding accessories kg 107 (net)THERMAL:Ambient temperature range °C +18 ... +30Temperature CO2 - equipmentcontrol range CO2 / O2 - equipment

°C°C

Ambient temp.+5 ... +50 +7 ... +50

Temperature deviation, temporal(DIN 12 880, Part 2) K < + 0.1Temperature deviation, at: 37°Cspatial (DIN 12 880, Part 2) 50°C

KK

< ± 0.5< ± 0.7

Heating-up time with auto-start (ambient temperature 22°C, unit empty)

to: 37°C h approx. 5Cooling time (ambient temperature 22°Cunit empty) from: 37 °C to 25°C h approx. 5Heat release into surroundings at 37°C / 95%rel. humidityHeat release into surroundings at 50°C / 95%rel. humidityHeat release into surroundings during disinfec-tion process

kWh/h

kWh/h

kWh/h

approx. 0,16

approx. 0,22

approx. 0,59

Recovery time at: 37°C / 5% CO2 95% rel. humidity / 7% O2(closing the doors after they have been openfor 30 s)

TemperatureCO2

HumidityO2

minminminmin

< 3< 2 (max. 1,4 % / min)< 9< 15

12. TECHNICAL SPECIFICATIONS

50 079 041 37/43

TECHNICAL SPECIFICATIONSUNITS: VALUE:

HumidificationWater quality

min. - max. level lDistilled or fully desalinated 2.2

Water demand to humidify:from 40 % rel. humidity to 95 % rel. humidity ml 17

Water consumption ml/h 10Total capacity l 4.7Measuring range and Setting range* % r.H. 60 ... 95Control accuracy % r.H. ± 1GAS ENGINEERINGCarbon dioxide (CO2)Oxygen (O2)Nitrogen (N2)PurityInlet pressure

%bar

99.50.8 up to max. 1

Measuring range and Setting range % CO2 0 ... 20Control accuracy % CO2 ± 0,1ELECTRICAL:Rated voltage Rated frequency

VHz

1/PE AC, 230 or 1/PE AC, 12050/60 50/60

Radio interference suppression (DIN VDE0875)

Interference suppression level N

Type of enclosure (DIN 40050) IP 20Protection class IOvervoltage category (IEC 1010) IIDegree of soiling (IEC 1010) 2Rated current A 5,2 or 10,0Fuse protection:

FuseCircuit-break switch

T 16 A slow-blowG 16

Rated power consumption duringincubation/disinfection

kW 1.2 / 1.2

MISCELLANEOUSSound pressure level (DIN 45635 Part 1) dB (A) < 50max. r.H of the ambiance % r. H 80Height m NN 2.000

* Under some circumstances, eg. when working with culture vessels without covers, the controlrange will be restricted to 90-95% since below this level evaporation of the medium will lead toan increase of the relative humidity in the chamber.

12. TECHNICAL SPECIFICATIONS

38/43

50 079 041

Components MaterialOuter housing Galvanized sheet steel, painted to RAL 9002Inner vessel, shelves Stainless steel, material No. 1.4301Glass pane Sodium silicate glassDoor seal of unit Magnetic core sheathed in soft PVCGlass door seal Tempered siliconThermal insulation PU foam sheet (CFC) incorporating non-woven needled

glass fibre quilt (binder-free)Control and display membrane PolyethyleneHeating systems Silicon-sheathed resistance heating conductor and tubu-

lar heaterLeads Plastic-sheathed copper strandsOther components Sheathed electrical components coated in various plas-

tics partly mounted on printed circuit boards bonded withepoxy resin

13. MATERIALS USED

50 079 041 39/43

Gas consumption during operationof "BBD 6220 gassed incubator"

Gas consumption corresponds to the gas quantity re-leased at the back of the unit which has to be dischargedsafely into the outside air by ventilating the placementarea.

14. GAS CONSUMPTION

40/43

50 079 041

15. PH-VALUE OF CULTURE MEDIA

50 079 041 41/43

pH-value of culture mediaDependency on the CO2-concentration

6,8 7 7,2 7,4 7,60

2

4

6

8

10

12

14

16

18

pH-value

% C

O2

Media Na HCO3 g/l

DMEM 3,70BME 2,20MEM 2,20Medium 199 2,20Mc Coy 2,20F 10 1,20F 12 1,20MEM 0,85Media with Hanks Salts 0,35

3,70

2,20

1,20

0,850,35

General rules

Keep windows and doors in the working areas closed while work is in progress.

Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas.

Lab coats or other protective garments must be worn in the working area.

Oral pipetting is prohibited. Use pipetting aids.

Only use needles and syringes if absolutely necessary.

With all manipulators, be sure to avoid aerosol formation as far as possible.

On completion of work and before leaving the working area, clean hands thoroughly. Disinfect and remois-turize hands if necessary.

Keep working areas clean and tidy. Only equipment and materials actually required should be on the work-tops. Keep stocks in the areas or cabinets provided for them only.

Regularly check the identity of the biological agents used if this is a requirement for hazard potential assessment. The length of the interval between checks should be set according to hazard potential.

With regard to the handling of biological agents, employees must be issued with verbal instructions relatingto the workplace before commencing work. These instructions must be re-issued at least once annuallythereafter.

Employees inexperienced in the fields of microbiology, virology or cellular biology must be given thoroughinstructions and be supervised.

Vermin and pests must be combatted regularly if necessary.

Handling of pathogenes

Also heed the following basic rules regarding the handling of pathogenes:Disinfect all workplaces on a daily basis. It may be necessary to switch disinfectants as a precautionagainst resistant germs.

Do not wear protective garments outside the working areas.

Contaminated equipment must be autoclaved or disinfected prior to cleaning.

Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or disinfection.

If infectious substances are spilt, the contaminated area must be closed off immediately and disinfected.

If work is conducted using human pathogenes against which an effective antidote is available, all employees who are not yet immune must be vaccinated and checked regularly for immunity in the appro-priate manner.

The health of employees must be monitored by medical check-ups, i.e. employees must undergo an initialcheck-up before commencing work and subsequent annual check-ups. Medical check-ups should be conducted in accordance with the applicable employers´ liability insurance association guidelines, in particular G 24, "Skin diseases", and G 42, "Infectious diseases". As generally recognized rules of indus-trial medicine, they enable the doctor to assess, evaluate and record the results of examinations accordingto the same criteria.

* Apply to cell cultures as appropriate

Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employer´s liability insurance as-sociation for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg

BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *

42/43

50 079 041

Potentially hazardous, genetically engineered organisms, viruses and subviral agents must be handled inaccordance with employers´ liability insurance association guideline G 43 entitled "Biotechnology".

Instructions for administering first aid in the event of accidents involving pathogenic microorganisms andviruses must be immediately to hand in the working area. The person in charge must be notified of all accidents immediately.

Depending on hazard potential, it may be necessary to take further safety precautions:

Use of class I, class II (type-approved)** or class III safety workbenches (air flow pointing away from experimenter).

Restricting and supervising access to specific areas.

Use of special protective garments and breathing apparatus.

Disinfection of all materials containing pathogenes before they leave the worktop.

Maintaining a partial vacuum in the working area.

Reducing the germ count in the exhaust air by taking appropriate measures, such as using high-performance suspended-matter filters.

Handling of human- and animal-pathogenic biological agents

The following general rules also apply to the handling of human- and animal-pathogenic biologicalagents:

Human-pathogenic biological agents may only be handled with approval under the terms of the FederalEpidemics Act.

Approval under the terms of the Federal Animal Epidemics Act and the Ordinance concerning Agents ofAnimal Epidemics is required to handle agents of animal epidemics.

Pregnant women or nursing mothers are not permitted to handle infectious and human-pathogenic biological agents or materials containing these agents.

* Apply to cell cultures as appropriate

**Manufacturers´ certificates can be found in the memoranda of the Berufsgenossenschaft Chemie (employer´s liability insurance association for chemicals) entitled "Sichere Chemiearbeit" (Safe chemistry) and the Berufsgenossenschaft für Gesundheitsdienst and Wohlfahrtspflege (employer´s liability insurance association for health service and welfare). They can also be obtained on request from the test centre of the expert committee on "Health care and welfare"(Berufsgenossenschaft für Gesundheitsdienst und Wohlfahrtspflege, Prüfstelle des Fachauschusses "Gesundheits-dienst und Wohlfahrtspflege, Pappelallee 35-37, 22089 Hamburg, Germany).

Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employer´s liability insurance as-sociation for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg

BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *

50 079 041 43/43

Stand: 10.2005 / 50 079 047 A

BBD 6220

ε ε

88

302

99

303

100

304

101

400

104

404

200

405

201

500

204

501

300

502

301

iε

i i

auto-start

≥10 min

%

%

CO2

°C

CO2

des

des

CO2

N2

N2

des

O2

O2

N2O2

Stand: 10.2005 / 50 079 047 A

BBD 6220 start

�

�

�

�

1

i

>30 s

max.

min.

aqua dest.

°C

>+ 2 °C

2

3

4

5

6

7

8

auto-start

0

I

°C

auto-start

> 5 s start/stop

&

%rH

%CO2

%O2

Stand: 10.2005 / 50 079 047 A

BBD 6220 des

�

�

�

�

i

>30 s

180 °C

t

auto-start

- auto-start

1

2

3

4

5

6

7

0

I

des start/ stop

>1 sec

Dokumentationssoftware Kelvi_Log für

cytoperm 2 und BBD 6220

50047979-C 1

Beschreibung

Die Dokumentationssoftware Kelvi_Log dient zur Aufzeichnung derBetriebsparameter eines Gerätes. Die Ausgabe der Parameter kannauf dem Bildschirm eines PC´s als Zahl, analog zu den Istwertanzei-gen am Gerät, oder als Istwert / Zeit-Graphik erfolgen. Die Aktualisie-rung der Werte erfolgt in einem wählbaren Meßintervall. Der Aus-druck der Daten kann in Form einer Tabelle und / oder einer entspre-chenden Graphik erfolgen. Für die Möglichkeit einer Weiterverarbei-tung der Daten, z.B. mit Tabellenkalkulationsprogrammen, wird eineentsprechende Datei erzeugt.

Systemvoraussetzungen:1. Microsoft - Windows, Version 3.x / 95 2. IBM-kompatibler PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (bei Windows 3.1) (Hinweis: Sollen andere Programme parallel aktiv sein, wird eine stärkere Ausstattung empfohlen)

Hinweis:Kendro Laboratory Products GmbH übernimmt keine Verantwor-tung für Schäden, die durch Fehlleistung der Software oder durchfehlerhafte Anwendung der Software entstehen.

Installation und BedienungVerbinden Sie Ihren PC mit dem Gerät über ein 9-poliges Schnittstel-lenkabel mit D-SUB Stecker (Zubehör). Die RS232 Schnittstelle be-findet sich an der Geräterückseite. An Ihrem PC können Sie die Ver-bindung über die Schnittstellen COM1 oder COM2 herstellen. DieLänge der Verbindungsleitung darf 3 Meter nicht übersteigen.

50047979-C 3

+ Kendro Laboratory

Products GmbH

Robert-Bosch-Str. 1

D - 63505 Langenselbold

( Vertrieb +49 (0) 618490 - 6940

Service +49 (0) 618490 - 6940

Vertrieb +49 (0) 618490 - 7474

Service +49 (0) 6184

90 - 7474

© Kendro Laboratory Products GmbH, 63505Langenselbold, Germany

Für Übersetzungen in Fremdsprachen ist die deutsche Fassung die-ser Betriebsanleitung verbindlich.Schutzgebühr

50047979-C 2

Installation:

1. Starten Sie Ihren Computer sowie Microsoft Windows2. Legen Sie die Diskette in Laufwerk A ein und öffnen Sie den Pro-gramm-Manager3. Wählen sie "Ausführen" aus dem Menü "Datei" und geben SieA:SETUP ein Das Setup-Programm eröffnet die Programmgruppe Kelvi_Log mitdem entsprechenden Programmsymbol, von dem aus das Pro-gramm gestartet werden kann.

Das Programm ist weitestgehend selbsterklärend. Für Zusatzinfor-mationen ist eine ausführliche "Hilfe"-Funktion enthalten. DieMenüführung kann in Deutsch oder Englisch erfolgen.

Hinweis:Soll das Programm nach einem Netzausfall automatisch wiedergestartet werden, so ist der Start von Windows in die DateiAUTOEXEC.BAT und das Kelvi_Log-Startsymbol in das WindowsAUTOSTART-Fenster mit aufzunehmen.

Das Setup-Fenster läßt sich jederzeit, auch von anderen Pro-grammen aus, mit der Taste F2 aufrufen.

Bestelldaten

Bestellnummer Artikel

50048122 Schnittstellenkabel, D-SUB, 9-polig, 3 Meterlang mit Adapter 9/25-polig

50047979-C 4

Documentation software Kelvi_Log for

cytoperm 2 and BBD 6220

50047979-C 1

Description

The Kelvi_Log documentation software is used to record the workingparameters of one appliance. The actual parameters can be dis-played on the screen of a PC as a number in the same way as it ison the feetback value display on the appliance, or it can be displayedas a feedback value / time chart. The actual value is updated at spe-cific measuring intervals, which can be selected. The data can beprinted in the form of a time / feedback value table and / or as a time /feedback value chart. A file is created which is suitable for furtherprocessing of the data, e.g., with a spread-sheet program.

System requirements:1. The software is run on Microsoft Windows (Version 3.x / 95).2. IBM compatible PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (using Windows 3.1) (Please note: when other programs should be run at the same time, a powerful equipment is recommended)

Please note:Kendro Laboratory Products GmbH can accept no responsibilityfor damages occurring as a result of software failure or incorrectuse of the software.

Installation and operationConnect your PC to the appliance with a 9 pin interface cable with aD-SUB connector (accessory). The RS232 interface is on the back ofthe appliance. You can connect the cable to your PC at the COM1 orCOM2 port. The connecting lead allowed not be longer than 3 me-tres.

50047979-C 3

+ Kendro Laboratory

Products GmbH

Robert-Bosch-Str. 1

D - 63505 Langenselbold

( Sales +49 (0) 618490 - 6940

Service +49 (0) 618490 - 6940

Sales +49 (0) 618490 - 7474

Service +49 (0) 6184

90 - 7474

© Kendro Laboratory GmbH, 63505 Langenselbold, Germany

The German version is binding for all translations of this instruction ma-nual into other languages.

Nominal charge

50047979-C 2

Installation:

1. Start your computer and Microsoft Windows2. Insert the diskette in drive A and open the Program Manager3. Select "Execute" from the "File" menu and enter A:SETUP The Set-up program opens the Kelvi_Log group of programs with therelevant window, from which the program can be started.

The program is largely self-explanatory. There is, however, a compre-hensive "Help" function available for additional information. The menuscan be run in German or English.

Please note: If you wish the program to be restarted automatically after powerfailure, the Windows start should be included in theAUTOEXEC.BAT file and the Kelvi_Log start symbol should beplaced in the AUTOSTART window.

Order number Item

50048122 Iterface cable, D-SUB, 9-pin, 3 metres longincl. adapter 9/25-pin

50047979-C 4

H e r a e u sINSTRUMENTS

0

I

5 . 0

7 . 0

3 7 . 0

95.0

° C

%CO2

% r H

i


D GB


0

I

5 . 0

7 . 0

3 7 . 0

95.0

° C

%CO2

% r H

i


Alarm Table Cytoperm 2/BBD 6220 Error Code Description Time to Alarm 88 Auto Start Error Up to 24 hours 99 Door Open too long 10 minutes 100 Temperature more than 0.5 degree

below set point a) 152 minutes from

switch on or change of set point.

b) 45 minutes after the door is closed

c) 1 minute continuously below.

101 Temperature more that 1-degree

above set point. a) 152 minutes from



c) 1 minute continuously above.

104 Temperature sensor faulty 1 minute 200/201 CO2 more than – or + 1% from set

point. a) 152 minutes from



c) 1 minute continuous.

204 CO2 Measuring cell faulty 1 minute 300/301/302/303 N2/O2 more that – or + 2% from set

point. a) 152 minutes from



c) 1 minute continuous.

304 O2 sensor faulty 1 minute 400 RH more than 5% below set point. a) 152 minutes from

switch on or change of set

point. b) 45 minutes after

the door is closed c) 1 minute

continuously below.

404 RH Sensor faulty 1 minute 405 Evaporator faulty, actual value less

than 95 degrees or more that 620 degrees.

1 minute

500/501 Temperature more than – or + 10 degrees from the set point

a) 152 minutes from switch on or change of set point.

d) 45 minutes after the door is closed

e) 1 minute continuous.

502 Error during disinfection cycle. 1 minute

Edition: 04.2003 / 50077611

Assembly Instruction

Fan change cytoperm 2® / BBD 6220

Fan change cytoperm 2 / BBD 6220

Edition 04/2003 / 50077611 - 2 -

Contents

Contents ..............................................................................................................................................2

1 General information ..................................................................................................................3

2 List of tools required..................................................................................................................3

3 Preperation................................................................................................................................. 3

4 Removing the fan and motor..................................................................................................... 4

4.1 Remove from the power ........................................................................................................... 4

4.2 Remove the fan ........................................................................................................................ 4

4.3 Removal of side panel .............................................................................................................. 5

4.4 Rear panel removal.................................................................................................................. 5

4.5 Remove insulation.................................................................................................................... 7

4.6 Fan motor removal .................................................................................................................. 7

4.7 Fitting the new fan and motor ................................................................................................. 9

4.8 Wiring the motor and PCB .................................................................................................... 11

4.9 Testing.................................................................................................................................... 13

4.10 Refitting the insulation........................................................................................................... 13

4.11 Refitting the rear panel .......................................................................................................... 13

4.12 Refitting the side panel........................................................................................................... 13

4.13 Chamber reassembly.............................................................................................................. 14

4.14 Put incubator into operation ................................................................................................. 14


Edition 04/2003 / 50077611 3

1 General information

This assembly instruction describes the change of the fan motor in cytoperm 2 and BBD 6220 CO2incubators with decontamination routine.

Only Kendro Service personnel or specialized personnel authorized by Kendro may perform theconversion. Having the conversion performed by parties other than those stated above will result inthe Kendro Laboratory Products GmbH warranty being voided.

After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must beperformed.

2 List of tools required

• Screwdrivers 1+2, System Philips Recess• Box spanners 10 mm and 13 mm• Pliers or adjustable grips• Special tool for aligning the spring sealing plate (Ident. 74215576 )• Side cutters• Knife

3 Preperation

The unit should be placed on a solid work surface (table)


Edition 04/2003 / 50077611 4

4 Removing the fan and motor

4.1 Remove from the power

1. Switch off the power.

2. Remove the mains cable.

4.2 Remove the fan

1. Remove all parts from the chamber

2. Remove the inner back wall cover

3. Using a 10 mm box spanner removethe nut holding the fan blade to themotor shaft CAUTION this is a lefthand thread.Pic 1 shows direction of spannerPic 2 shows fan blade removed

Pic 1

Pic 2


Edition 04/2003 / 50077611 5

4.3 Removal of side panel

1. Remove screws from the side paneland from the rear – Pic 3

Pic 3

4.4 Rear panel removal

1. The following components must beremoved before the back panel can beremoved:

1. Over pressure tube

2. Cover of evaporater ovenexhaust

3. Fan cover

Pic 4

2. Unscrew the over pressure tube Pic 5

Pic 5

Attention: Also from the rear


Edition 04/2003 / 50077611 6

3. Remove the screws and take off theexhaust cover Pic 6

Pic 6

4. Remove the screws and take off thefan motor cover Pic 7

Pic 7

5. Remove screws and take off thecomplete back panel Pic 8

Pic 8


Edition 04/2003 / 50077611 7

4.5 Remove insulation

1. Remove the insulation from the rear ofthe incubator

Pic 9

4.6 Fan motor removal

1. Remove the electrical connectors fromthe motor 2x power, and 1x earth Pic10

Pic 10

2. Unscrew 4 x 13 mm motor mountingnuts Pic 11

Pic 11

3. Remove motor and housing

Electricalconnectors


Edition 04/2003 / 50077611 8

4. Using pliers remove the motor supportstuds.If the rockwool insulation becomesdetached affix with silicone beforefitting the new studs.

Pic 12

5. Fit the new motor support studs andtighten, hand tight Pic 13

Pic 13

Support studs


Edition 04/2003 / 50077611 9

4.7 Fitting the new fan and motor

1. Cut the grommet in the new motorhousing as shown by the cross belowPic 14

Pic 14

2. Feed the cable with the greenconnector through the grommet Pic 15

Pic 15

3. Plug the green connector into thesocket Pic 16

Pic 16

Grommet

Socket


Edition 04/2003 / 50077611 10

4. Feed the motor supply cables throughthe grommet, 1 x earth and 2 x powerPic 17

Pic 17

5. Fit the motor and housing to the motorsupport studs and tighten the nutsusing a 13 mm spanner Pic 18

Pic18

6. Push the special alignment tool againstthe spring plate and ensure that platesits flat against the motor housing wall.Pic 19

Pic 19


Edition 04/2003 / 50077611 11

4.8 Wiring the motor and PCB

1. Feed the cables from the motor intothe electronics compartment in parallelwith existing wires.

1. Cut a slot in the insulationwith a sharp knife Pic 20

2. Feed the cables through theinsulation Pic 21

Pic 20

3. Tie the new cables to theexisting cables using thewrap binding provided Pic22 Tie to the cabling in theelectronics compartmentusing a cable tie Pic 23

Pic 21

Pic 22

Pic 23

Wrap binding

Cable tie

Clamp


Edition 04/2003 / 50077611 12

2. Fit the fan speed PCB onto the mainboard using cable ties as shownPic 24

Pic 24

3. Connect the electrical cables betweenthe fan speed PCB and the main boardPic 25

Mainboard Fan speed PCB X 19 à XA 119 X 20 à XA 120

Pic 25

4. Connect the control cable from the fanmotor to connector X2 of the fan speedPCB

5. Fasten the cable with a cable tie

6. If the software version of the EPROMis older than 28.11.2002, thenexchange the EPROM

Attention: Pay attention to theorientation of the EPROM(cut out down)

Control cable X 2


Edition 04/2003 / 50077611 13

4.9 Testing

1. Test the function of the new motorwhen built into the incubator

2. Reconnect the mains supply andswitch on

3. After all the 8’s are displayed the fanmust run

4. If the fan motor fails to turn or does notrotate at reduced speed, check that theabove steps have been completedcorrectly (especially the alignment ofthe motor)

5. For aktivating the speed control infunction level 601 underlevel 3 thevalue must be enter to 10 = speed control deaktivate1 = speed control aktive

6. After the successful function test,switch the incubator off and disconnectfrom the mains

4.10 Refitting the insulation

1. Follow the steps in 5.5 in reverse order

4.11 Refitting the rear panel

1. Follow the steps in 5.4 in reverse order

4.12 Refitting the side panel

1. Follow the steps inl 5.3 in reverseorder


Edition 04/2003 / 50077611 14

4.13 Chamber reassembly

1. Install the fan1. Fit the large washer on the

motor shaft (Pic 26)2. Fit the fan3. Fasten with the nut

Bild 26

2. Refit the inner rear wall

3. Fit all other chamber parts

4.14 Put incubator into operation

1. Reconnect to mains power

2. Switch on

3. Follow instructions in operator manual

4. Enter control parameters (if main boardhas been replaced)

5. Carry out auto-start

After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must beperformed.


Edition 04/2003 / 50077611 15

Anschrift:Kendro Laboratory Products GmbHRobert - Bosch – Straße 1D - 63505 LangenselboldDeutschland

Telefon:Vertrieb + 49 (0) 1805-536376Service + 49 (0) 1805-112110

Fax:Vertrieb/Service + 49 (0) 1805-112114

Assembly Instruction Water tank change cytoperm 2® / BBD 6220

Stand 05/2003 / 50077825

Wassertankwechsel Cytoperm 2 / BBD 6220

Index

1 General information ..................................................................................................................2

2 List of tools required..................................................................................................................2

3 Preparation.................................................................................................................................2

4 Demounting the water tank.......................................................................................................3 4.1 Remove the power ...............................................................................................................3 4.2 Remove side panel ...............................................................................................................3 4.3 Remove water tank...............................................................................................................4

5 Fitting the water tank with heating ..........................................................................................5

6 Installation of regulator panel ..................................................................................................6

7 Electrical connections ................................................................................................................7

8 Fitting the tubes..........................................................................................................................9

9 Testing the water tank heating ...............................................................................................10

10 Refit the side panel...............................................................................................................10

11 Return to operation..............................................................................................................11

Stand 05/2003 / 50077825 Seite - 1 -


1 General information This assembly instruction describes the change of the water tank in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.

2 List of tools required • Screwdrivers 1+2, System Philips Recess • Ratchet spanner SW 13 • Adjustable pliers • Power drill with 3 mm drill • Side cutters • Crimp tool • Ruler

3 Preparation The instrument must be placed on a strong work surface

Stand 05/2003 / 50077825 Seite - 2 -


4 Demounting the water tank

4.1 Remove the power 1. Switch unit off

2. Remove power cable

4.2 Remove side panel 1. Remove screws from side panel (Pic 1)

Pic 1

Attention: Also the screws at the back

Stand 05/2003 / 50077825 Seite - 3 -


4.3 Remove water tank 1. Drain the water

2. Remove the tubing from the water tank (Pic 2), 2x from the water valve (Pic 3) also from the filling and overflow ports on the front panel (Pic 4)

Pic 2

Pic 3

Pic 4

3. Remove the three bolts (Pic 5) and then remove the water tank

Pic 5

Stand 05/2003 / 50077825 Seite - 4 -


4. Replace the water valve (Pic 6)

Pic 6

5 Fitting the water tank with heating The new water tank comes with an

integral heater and temperature regulators attached. The temperature and over temperature regulators are on a separate mounting assembly (Pic 7)

Pic 7

1. Fit the two tubes to the top of the water tank (Pic 8)

Pic 8

2. Mount the water tank with 3 new (longer) bolts (Pic 9)

Pic 9

Stand 05/2003 / 50077825 Seite - 5 -


6 Installation of regulator panel 1. Mark the position of the holes for

mounting the regulator panel (Pic 10)

Pic 10

2. The position of the lower hole should be 4 cm from the bottom of the support rail (Pic 11)

Pic 11

3. Drill the mounting holes with a 3mm drill (Pic 12)

Pic 12

Regulator panel

4 cm from the bottom of the support rail

Stand 05/2003 / 50077825 Seite - 6 -


4. Mount the regulator panel using 3 mm self tapping screws with star washers (Pic 13)

Pic 13

5. Attention: There should be sufficient clearence between the relay and the main board (Pic 14)

Pic 14

7 Electrical connections 1. Connect the earth wire (green/yellow)

between the earth connector on the regulator panel and the instrument ground

2. Connect the mains wires ( 2 x black) to the transformer, connector 1 & 3 (Pic 15)

Pic 15

3. 1. Remove the two wires (yellow & orange) from terminals 9 & 10 of connector X 16 on the main board

Connector 1 & 3

Stand 05/2003 / 50077825 Seite - 7 -


2. Connect the two control wires

(blue) with sleeve markings 169 & 1610 to terminals 9 & 10 of X 16 on the main board Cable 169 to X16:9 Cable 1610 to X16:10 (Pic 16)

3. Using a crimp tool connect the 2 control wires (blue) marked 1 & 2 to the orange and yellow wires previously removed from connector X16 Cable 1 to yellow Cable 2 to orange (Pic 17)

4. Take the two heating wires from the new water tank and connect to the regulator panel pcb (Pic 18)

Pic 16

Pic 17

Pic18

4. Route the cables tidily using cable ties

Stand 05/2003 / 50077825 Seite - 8 -


8 Fitting the tubes 1. Connect the tubes to the water tank

(Pic 19)

Pic 19

2. Connect the other end of tube to the water valve (Pic 20)

Pic 20

3. Fit the tubing to the fill port on the front panel. Secure using a cable tie

4. Fit the tubing to the overflow port on the front panel. Secure using a cable tie

5. Fit the tube with the capillary to the underside of the water valve, connect the other end to the evaporator oven

6. Arrange the tubes tidily inside the control panel

Stand 05/2003 / 50077825 Seite - 9 -


9 Testing the water tank heating 1. Connect the power cable and switch

the incubator on

2. The upper regulator provides primary control for the water heating and the lower regulator over temperature protection

3. Turn both regulators fully clockwise

4. Fill the water tank

5. Initiate an Auto-Start

6. Cancel the Auto-Start

7. Canceling the Auto-Start will initiate an Auto-Zero. The water heating is triggered by the Auto-Zero Signal and can be detected by listening for the sound of the water heating relay closing

8. Turn the upper regulator anticlockwise until the relay drops out – reset the regulator to the fully clockwise position

10 Refit the side panel 1. Follow 4.2 in in reverse order

Stand 05/2003 / 50077825 Seite - 10 -

Montageanleitung

Assembly Instruction Softwareaustausch cytoperm 2® / BBD 6220 Software Change cytoperm 2® / BBD 6220

Stand: 12.2004 / 50083482

Softwareaustausch Cytoperm 2 / BBD 6220

Inhaltsverzeichnis:

1 Allgemeiner Hinweis ..................................................................................................................2

2 Liste der benötigten Werkzeuge und Hilfsmittel ....................................................................2

3 Vorbereitung...............................................................................................................................2

4 Austausch der Software.............................................................................................................3

5 Softwareänderungen..................................................................................................................4

6 General Information..................................................................................................................5

7 List of Tools Required ...............................................................................................................5

8 Preparation.................................................................................................................................5

9 Changing the Software ..............................................................................................................6

10 Software Changes...................................................................................................................7

Seite - 1 -


1 Allgemeiner Hinweis Diese Montageanleitung beschreibt den Austausch des Software bei den CO2 Inkubatoren cytoperm 2 und BBD 6220 mit Dekontaminationsroutine. Die Umrüstung des Gerätes darf nur vom Kendro Service oder von Kendro autorisiertem Fachpersonal durchgeführt werden. Erfolgt die Umrüstung nicht durch den Service, entfällt die Gewährleistung seitens Kendro Laboratory Products GmbH. Nach Durchführung muss eine Elektrische Sicherheitsprüfung nach DIN VDE 0701 Teil 1 erfolgen.

2 Liste der benötigten Werkzeuge und Hilfsmittel • Schraubendreher • Ersatz EPROM 50083354 mit Software Version 50065109 – C / 09.12.2004

3 Vorbereitung Das Gerät sollte sich auf einer stabilen Unterlage (Tisch) befinden.

Seite - 2 -


4 Austausch der Software

Werte aus folgenden Funktionsebenen auslesen und protokollieren FE 644 Unterebenen 1....5 FE 645 Unterebenen 1....14 FE 646 Unterebenen 1....7 bei O2 Geräten EPROM tauschen

Markierung am EPROM

In der Funktionsebene 601 die Unterebene 2 von 0 auf 1 und Unterebene 4 von 0 auf 1 setzen. Nach dem Loslassen der Tasten wird automatisch ein RESET ausgeführt

o Nur bei O2 Geräten: In der Funktionseben 601 die Unterebene 2 von 0 auf 1 und Unterebene 1 von 1 auf 2 setzen (einstellen der Gerätevariante) Nach dem Loslassen der Tasten wird automatisch ein RESET ausgeführt

Seite - 3 -


Die Funktionsebene 601 Unterebene 3 konfigurieren.

Funktionsebene 601 Unterebene 3 auf 0 – Drehzahlregelung aus Funktionsebene 601 Unterebene 3 auf 1 – Drehzahlregelung ein Die protokollierten Werte aus den Funktionsebenen 644, 645 und 646

(bei O2 Geräten) wieder eingeben. In der Funktionsebene 999 sollen folgende Einträge zu sehen sein:

Unterebene 1 - 5 Unterebene 2 - 11 Unterebene 3 - 4 Sollwerte eingeben

Gerät mit Auto-Start in Betrieb nehmen.

5 Softwareänderungen

• Funktionsebene 1 Unterebene 2 entfällt. • Funktionsebene 601 Unterebene 4 hinzugefügt Laden von Default Parameter

nach dem Tausch des EPROMs. • Die Netznullpunkterkennung für die geregelte Drehzahlsteuerung ist optimiert. • Der Fehler 400 (Relative Feuchte unter Sollwert; Istwert < Sollwert –5%) wird nach

dem erneuten Erreichen des Fehlerbandes zurückgesetzt. • Die Fehlerbänder der Temperatur betragen –1K und +2 K. • Die Stellgröße für die Heizung ist auf maximal 30% begrenzt. • Die Türheizung wird während der Türöffnung nicht angesteuert. • Die Verdampferofen Temperatur beträgt 450°C, die Einschalttemperatur für die

Befeuchtung beträgt 420°C

Seite - 4 -


6 General Information This assembly instruction describes the change of the software in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.

7 List of Tools Required • Screwdriver • Spare EPROM 50083354 with Software Version 50065109 – C / 09.12.2004

8 Preparation The instrument must be placed on a strong work surface.

Seite - 5 -


9 Changing the Software

Enter the following function levels and record the values FL 644 Sub levels 1-5 FL 645 Sub levels 1-14 FL 646 Sub levels 1-7 for O2 Units only. Change EPROM

Notch of EPROM

In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 4 Change 0 to 1 When the button is released an automatic RESET will be carried out.

o Only for units equipped with O2 control.

In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 1 Change 1 to 2 (sets the unit variant)

When the button is released an automatic RESET will be carried out.

Seite - 6 -


Seite - 7 -

Function Level 601 Sub Level 3 configures:

FL 601 Sub Level 3 to 0 – Fan speed control off. FL 601 Sub Level 3 to 1 – Fan speed control on. Enter the previously recorded values in 644, 645

and 646 (only for O2 units). The software version may be viewed In Function Level 999 :

Sub Level 1 - 5 Sub Level 2 - 11 Sub Level 3 - 4 Enter customer set points.

Carry out Auto Start.

10 Software Changes

• Function Level 1 Sub Level 2 removed. • Function Level 601 Sub Level 4 added loading of default parameters after an

EPROM change. • Optimisation of the mains zero crossing point for the fan speed controller. • Error 400 (Relative Humidity low; current value < set value – 5%) will be reset after

the current value reaches the error band again. • The error band for temperature is now –1K and +2 K of set point. • The control variable for the heating is now limited to a maximum of 30%. • The door heating is not active whilst the door is open. • The evaporator temperature is 450°C, the starting temperaure for humidifying is

420°C

Anschrift: Kendro Laboratory Products GmbH Robert-Bosch-Strasse 1 D – 63505 Langenselbold Deutschland Telefon: Sales + 49 (0) 800-1-536 376Service + 49 (0) 800-1-112 110 Fax: Sales/ Service + 49 (0) 800-1-112 114

BETRIEBSANLEITUNGHERAline

Seite 6 von 10Datum: 03.11.00

Bearbeiter / Abteilung: Identnummer: interner Vermerk

Christina Meier / EP 1 50 055 159 50055159 dt gbvers3.doc

5. Desciption for BBD 6220 and cytoperm 2

The HERAline analog interface converts the digital values of temperature, CO2,humidity and oxygen (if installed) from the RS 232 interface of the incubator in 3 or 4analog signals, having 0....1 VDC, by using a 4 channel D/A converter together with amicroprocessor. The resolution of the D/A conversation is 10 bit for each channel,which means totally 1024 steps. Since the RS 232 will be used for the HERAlineinterface, it will not be possible to connect an additional computer to the RS 232interface of the incubator.

The analog outputs are connected to a female 15 pole D-Sub connector, which islocated at the rear of the incubator. External devices can be connected to theinterface by using the standart RGB/S video cable which commes with the HERAlineinterface.

The analog output signals are:

Red „R“ Temperatureincubation modeTemperaturedesinfection mode

20 mV/°C

5 mV/°C

0...50 °C

0...200°C

=

=

0...1 VDC

0...1 VDC

The output signal will be switched over automatically from 20 mV/°C to 5 mV/°C after starting thedesinfection mode and back from 5 mV/°C to 20 mV/°C after the desinfection mode has been finished.

Green „G“ CO2 - concentration 50 mV/% 0...20% CO2 = 0...1 VDC

Blue „B“ Humidity 10 mV/% 0...100% rH = 0...1 VDC

Black „S“ O2 - concentration 10 mV/% 0...100% O2 = 0...1 VDC

It will be possible to connect the external devices directly to a male 15 pole D-Subconnector which fits to the female output connector of the incubator.

The outputs are connected to:

Temperature Pin 1 (+)

CO2 Pin 2 (+)

Humidity Pin 3 (+)

O2 Pin 13 (+)

Ground (-) Pin 4,6,7,10 und 11 (-)





6. Mounting instructions for HERAline to BBD 6220 and cytoperm 2

1. Check the manufacturer’s number on the nameplate. For manufacturer’s numberslarger than 20 000 000, start with item 6 of this instruction. For manufacturer’snumbers lower than 20 000 000, perform an EPROM software test. Proceed asfollows:

- Press and hold down the Keyswitch. Press the ? key until 999 appears in theDisplay for °C (level 999).

- Release both keys.

- Press the Keyswitch.

The “Day” of the software appears in the Display for °C. 1 appears in theDisplay for %CO2, indicating sublevel 1.

- Press the Keyswitch..

The “Month” of the software appears in the Display for °C. 2 appears in theDisplay for %CO2, indicating sublevel 2.


The “Year” of the software appears in the Display for °C. 3 appears in theDisplay for %CO2, indicating sublevel 3.

- To exit the menu, press either the °C key; the %CO2 key, or the rH key.

If the indicated software level is prior to 07/13/98, the EPROM must bereplaced.To do this:

2. Disconnect the unit from the power supply..

3. Open the side cover of the incubator.

4. Replace the EPROM (U2) on the main PCB. (If the EPROM’s date is, forexample, 07/13/98). Note the polarity (tab).

5. Close the side cover.

6. Using a Philip’s head screwdriver, open the HERAline analog coupler and setthe bridge assignment for S1 in accordance with the included drawing 50 053075.





7. Close the analog coupler.

8. Connect the interface cable between the RS 232 port on the incubator and theHERAline (digital Input).

9. Connect the RGB/S cable to the HERAline (analog output).

10. Connect the adapter (BNC plug / banana plug) to the RGB/S cable.

11. Connect the power pack.

12. Connect the unit to the mains outlet.

13. The analog output signals can now be tapped from the laboratory sockets.




Detlef Dornseiff / DT 50 076 300 50076300 IndexA.doc

7. Desciption for BBD 6220 and cytoperm 2

The HERAline analog interface converts the digital values of temperature, CO2,humidity and oxygen (if installed) from the RS 232 interface of the incubator in 3 or 4analog signals, having 4....20 mA, by using a 4 channel D/A converter together with amicroprocessor. The resolution of the D/A conversation is 10 bit for each channel,which means totally 1024 steps. Since the RS 232 will be used for the HERAlineinterface, it will not be possible to connect an additional computer to the RS 232interface of the incubator.

The analog outputs are connected to a female 15 pole D-Sub connector, which islocated on the analog interface. External devices can be connected to the interfaceby using the standart RGB/S video cable which commands with the HERAlineinterface.

The analog output signals are:

Red „R“ Temperatureincubation modeTemperaturedesinfection mode

0,32 mA/°C

0,08 mA/°C

0...50 °C

0...200°C

=

=

4...20 mA

4...20 mA

The output signal will be switched over automatically from 0,32 mA/°C to 0,08 mA/°C after starting thedesinfection mode and back from 0,08 mA/°C to 0,32 mA/°C after the desinfection mode has beenfinished.

Green „G“ CO2 - concentration 0,8 mA/% 0...20% CO2 = 4...20 mA

Blue „B“ Humidity 0,16 mA/% 0...100% rH = 4...20 mA

Black „S“ O2 - concentration 0,16 mA/% 0...100% O2 = 4...20 mA

It will be possible to connect the external devices directly to a male 15 pole D-Subconnector which fits to the female output connector of the incubator.

The outputs are connected to:

Temperature Pin 1 (+)

CO2 Pin 2 (+)

Humidity Pin 3 (+)

O2 Pin 13 (+)

Ground (-) Pin 4,6,7,10 und 11 (-)





8. Mounting instructions for HERAline to BBD 6220 and cytoperm 2

1. Check the serial number on the nameplate. For serial numbers above than20 000 000, start with item 6 of this instruction. For serial numbers below than20 000 000, perform an EPROM software test. Proceed as follows:

- Press and hold down the Keyswitch. Press the ? key until 999 appears in theDisplay for °C (level 999).

- Release both keys.


The “Day” of the software appears in the Display for °C. 1 appears in theDisplay for %CO2, indicating sublevel 1.

- Press the Keyswitch..

The “Month” of the software appears in the Display for °C. 2 appears in theDisplay for %CO2, indicating sublevel 2.


The “Year” of the software appears in the Display for °C. 3 appears in theDisplay for %CO2, indicating sublevel 3.

- To exit the menu, press either the °C key; the %CO2 key, or the rH key.

If the indicated software level is prior to 07/13/98, the EPROM must bereplaced.To do this:

2. Disconnect the unit from the power supply..

3. Open the side cover of the incubator.

4. Replace the EPROM (U2) on the main PCB. (If the EPROM’s date is, e.g.,07/13/98). Note the polarity (tab).

5. Close the side cover.

6. Using a Philip’s head screwdriver, open the HERAline analog interface andset the jumper assignment for S1 in accordance with the included drawing50 053 075.

7. Close the analog interface.





8. Connect the interface cable between the RS 232 port on the incubator and theHERAline (digital Input).

9. Connect the RGB/S cable to the HERAline (analog output).

10. Connect the adapter (BNC plug / banana sockets) to the RGB/S cable.

11. Connect the power pack.

12. Connect the unit to the mains outlet.

13. The analog output signals are now avaiable on the banana sockets.

5

5

4

4

3

3

2

2

1

1

D D

C C

B B

A A

L

N

GND

Zeichnungsnummer RevLaboratory Products

Kendro A4Blatt

von

Bearb.Gepr.

Norm.Datum Name

LP bestückt:LP unbestückt:

Ind. DatumÄnderung Name A

50076074B

1

1

Leiterplatte Biofouling-

DickTuesday, December 17, 2002

17.12.02

50076075

50076073A

X16.9Signal AutoZeroX16.10 N

L

Schalter N1

Schalter N2

Anschluß Heizung

Netzeingang

HOLE3

HOLE4.5/8.01

K155.13.8.230.0000

85

1314

74 1 2

96 3

CR3

Crop_Mini

HOLE4

HOLE4.5/8.01

R13k3

X2

WA

GO

_236

12

X3

FS

900-

212

C R4

Crop_Mini

X4

FS

900-

212

LP1

Leiterplatte 50076073

X1

WA

GO

_236

12

HOLE1

HOLE4.5/8.01

RC10,1uF/100R

12

CR1

Crop_Mini

X5

FS

900-

212

D1BRT22H 1+6

612 4

HOLE2

HOLE4.5/8.01

X6

FS

900-

212

CR2

Crop_Mini

Leiterplatte Biofouling Revised: Tuesday, December 17, 200250076074-B

Kendro GmbHThorsten DickHeraeusstr. 12-14D-63450 HanauGermany

Item Quantity Part Hersteller Bestellbezeichnung Package Type Kommentar Reference Bestückung

1 4 Crop_Mini CR1,CR2,CR3,CR42 1 BRT22H 1+6 Siemens BRT22H Option 1+6 DIL6L 8mm D13 4 HOLE4.5/8.0 HOLE1,HOLE2,HOLE3,HOLE44 1 55.13.8.230.0000 Finder 55.13.8.230.0000 230V/3x10A K15 1 Leiterplatte 50076073A LP16 1 0,1uF/100R Röderstein 26x8mm Funkentstörglied 250VAC RC17 1 3k3 Neohm ??? 0.66W 350V 5% R18 2 WAGO_236 WAGO 236-402 WAGO Cage Clamp 2-polig X2,X19 4 FS900-2 WECO 47.870.903 2-polig X3,X4,X5,X6

Spare Parts List

Name/Name Datum/Date Benennung/Designation

Erstellt/Prepared D.Dornseiff 11.11.04Index Änderung Name/Name Datum/Date

Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2

Dokumentnr./Document No. Seite/Page

Datei/File Spare Parts list (E).doc 50 042 725 1 / 3 Weitergabe sowie Vervielfältigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrücklich zugestanden. Zuwiderhandlungen

verpflichten zu Schadensersatz. Alle Rechte für den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. © Kendro Laboratory Products GmbH, Germany

Part number

Description Qty.

Front Panel Components 03 001 359 Indicator lamp, green (mains) 1 50 047 901 Mains switch BBD 6220 1 03 002 308 Mains switch Cytoperm 2 (key switch) 1 50 011 623 Indicator lamp, red (over temperature) 1 50 011 688 Indicator lamp, white (disinfection) 1

50 047 902 50 047 903 50 047 906 50 047 907

Pushbutton, disinfection BBD 6220 (Pushbutton consists of): - Pushbutton switch - Connector - White cover - Switch cover

1 1 1 1

03 002 594 Key switch, disinfection Cytoperm 2 1 50 047 621 Front foil for BBD 6220 CO2 only. 1 50 047 622 Front foil for BBD 6220 CO2 & O2 1 50 042 579 Front foil for Cytoperm 2, CO2 only 1 50 043 159 Front foil for Cytoperm 2 CO2 & O2 1 Electrical Components 03 672 259 Main PCB, 50 Hz 1 03 672 287 Main PCB, 60 Hz 1 03 672 260 Display PCB 1 50 043 137 Transformer 1 50 047 060 Membrane Pump, Auto-Zero & Humidity 2 03 002 959 Springs for Membrane Pump ( 3 per pump) 6 50 043 508 Magnetic Valve CO2 und O2 1 & 1 50 048 298 Tube Connector for CO2 Inlet 1 50 044 831 Tube Connector for O2 Inlet 1 03 667 990 Seal for Inlet Connector 2 03 774 775 Schlauchtülle für Ventilausgang 2 50 043 214 Mains Filter 1 03 002 641 Fuse T 6,3 A 2 50 031 647 Disinfection Over Temperature Protection 1 50 031 323 Water Container Regulator 2 Water Line Components 1 50 029 129 Filling Connector (white) 1 03 002 869 Overfill Connector (black) 1 50 076 562 Water Container Complete, Including Heaters 1 50 054 606 Water Filter 1 50 052 465 Water Magnetic Valve 1







50 052 764 Water Valve Rubber Mount 2 50 054 076 Water Tubing Kit 1 50 054 606 Y Piece 1 26 136 275 Capillary Tube 1 03 672 272 Evaporator Oven 1 50 035 329 Thermo element NiCr – Ni for Evaporator

Oven 1

50 030 094 Tube for Evaporator Oven 1 Sensors (Electrical Panel & Rear Wall) 50 044 960 O2 Sensor Complete. 1 50 035 309 Humidity Sensor Complete. 1 50 035 304 Seal for Humidity Sensor 1 50 028 621 PT 100 Temperature Sensor 2 50 057 745 Silicone Grommet 2 03 672 263 CO2 Sensor 1 50 054 077 Heating Resistor for CO2 Sensor 1 50 043 902 Cable for TCD 1 50 035 294 Disinfection Heater 1 50 076 706 Ventilator fan upgrade kit 1 50 065 196 Ventilator Motor (new) 1 Outer Door / Glass Door / Gas Screen 50 025 445 Glass Door Complete 1 03 762 308 Latch for Glass Door & Gas Screen Door 1 50 046 210 6 Door Gas Screen Complete/Upgrade Kit 1 50 030 020 Glass door left for 6 door glass screen 2 50 030 021 Glass door left for 6 door glass screen with

hole 1

50 030 022 Glass door right, for 6 door glass screen 3 26 139 262 Septum 1 50 070 610 Seal for Glass Door / Gas screen 1 50 041 536 Seal for 6 door glass screen 6 03 001 322 Catch with Spring Cap, 6 Door Screen 1 03 001 332 Washer for Spring Catch, 6 Door Screen 1 50 029 928 Door Catch (Right), 6 Door Screen 3 50 029 929 Door Catch (Left), 6 Door Screen 3 50 079 959 Outer Door (with lock), BBD 6220 1 50 079 960 Outer Door (with lock), Cytoperm 2 1 50 080 010 Outer Door (without lock) BBD 6220 1 50 080 011 Outer Door (without lock), Cytoperm 2 1 50 011 356 Magnetic door Seal 1 Sonstiges 500 44 921 Foot 4 50 029 587 Foot Height Adjustable 4 50 044 920 Lock Washer 4 03 005 774 Fixing Screw for Foot 4







03 746 754 Door switch 1 50 055 142 Stopper for Access Port (with Hole) 1 03 985 223 Filter for CO2 und O2 2 50 034 771 Plug for Potential Free Alarm Contact 1 50 034 772 Angled Plug for Potential Free Alarm Contact 1 50 043 143 Mains Cable (EU) 1 50 047 099 Mains Cable (CH) 1 50 047 100 Mains Cable (GB) 1 50 068 738 Mains Cable (AUS) 1 50 072 450 Mains Cable (DK) 1 03 840 505 Mains Cable (USA) 1 50 029 126 Water Filling Canister 1 50 065 067 Seal for Water Filling Connection 1 50 062 701 Gas Tubing Set (external). 1 03 670 005 Air filter 2 03 651 850 Rubber Cover for Air Filter 2 50 069 923 Full Width Shelf (1 mm) 3 50 074 093 Strengthened Full Width Shelf (2 mm) 3 50 069 921 ½ Width Shelf 6 50 011 380 Shelf Support 6 o.12 50 011 398 Middle Support for Shelf Complete 2 Tubing Shown in the Tubing Diagram 03 650 438 Silicone tube 12 x 2 036 50 437 Silicone tube 10 x 2 50 044 922 Silicone tube 4 x 2 50 029 128 Viton tube 6 x 1.5 03 650 435 Silicone tube 4 x 1 03 650 428 Silicon tube 8 x 2 03 650 434 Silicon tube 3 x 2 50 047 880 Tube Clamp D = 10 50 047 881 Tube Clamp D = 8

Version: 07.2003 / 50078754

Accessories for CO2 - incubators

Illustrations and descriptions


Stand 07.2003 / 50078754 2

List of contents

List of contents ................................................................................................................................... 2

1 Accessories for HERAcell / HERAcell 150 ..............................................................................3

1.1 Support frames..................................................................................................................... 3

1.2 Shelves / HERAtrays ............................................................................................................ 4

1.3 Stacking adapters ................................................................................................................ 4

2 Accessories for HERAcell 240................................................................................................... 5

2.1 Support frames..................................................................................................................... 5

2.2 Shelves / HERAtrays ............................................................................................................ 6

2.3 Stacking adapters ................................................................................................................ 7

3 Accessories for cytoperm 2 / BBD 6220 ...................................................................................8

3.1 Support frames..................................................................................................................... 8

3.2 Shelves / HERAtrays .......................................................................................................... 10

3.3 Stacking adapters .............................................................................................................. 11


Version: 07.2003 / 50078754 3

1 Accessories for HERAcell / HERAcell 150

1.1 Support frames

1. Support frame 200 mmWidth: 637 mmHeight: 200 mmDepth: 600 mm

50 051 376


50 051 436

3. Support frame 185 mm (with castors)Width: 637 mmHeight: 185 mmDepth: 600 mm

50 057 161

4. Support cart with drawers andcastorsWidth: 637 mmHeight: 890 mmDepth: 600 mm

50 056 459

5. Castor set for 1 & 2 above. (Set of 4)

Information:When fitted the castors will raise thesupport frame by 90 mm.

50 052 528


Version: 07.2003 / 50078754 4

1.2 Shelves / HERAtrays

1. Shelf inc. support rails, SSShelf inc. support rails, CuWidth: 423 mm (OutsideDepth: 465 mm (Outside)Thickness: 1 mm

50 051 90950 051 910

2 HERAtray 1/3 Width SS (3 pc.)HERAtray 1/3 Width Cu (3 pc.)Width: 135 mm (Overall)Depth: 440 mm (Overall)Thickness: 1 mm

50 051 91350 051 914

3. HERAtray 1/2 Width SS (2 pc.)HERAtray 1/2 Width Cu (2 pc.)Width: 205 mm (Overall)Depth: 440 mm (Overall)Thickness: 1 mm

50 058 67250 061 050

1.3 Stacking adapters

1. Plate for stacking a HERAcell orHERAcell 150 on a HERAcell 240Width: 780 mmDepth: 772 mm

50 068 677

2. Plate for stacking a HERAcell orHERAcell 150 with a BB 16Width: 647mmDepth: 660 mm

50 051 938

3. Plate for stacking a HERAcell orHERAcell 150 on a BB 6220Width: 920 mmDepth: 670 mm

50 060 612

4. Plate for stacking a HERAcell orHERAcell 150 on a 5060 or B 5061Width: 890 mmDepth: 665 mm

50 060 736


Version: 07.2003 / 50078754 5

2 Accessories for HERAcell 240

2.1 Support frames


50 065 754


50 065 753

3. Support frame 185 mm (with castors)Width: 780 mmHeight: 185 mmDepth: 652 mm

50 067 224

4. Castor set for 1 & 2 above (set of 4)

Information:When fitted the castors will increasethe height of the support frame by 90mm.

50 052 528


Version: 07.2003 / 50078754 6


1. Standard shelfFull width inc. support rails, SSFull width inc. support rails, CuWidth: 560 mm (Overall)Depth: 500 mm (Overall)Thickness: 1 mm

50 065 79350 065 794

2. Reinforced shelf (2 mm Thick)Full width inc. 2 support rails, SSFull width inc. 2 support rails, CuWidth: 560 mm (Overall)Depth: 500 mm (Overall)Thickness: 2 mm

50 077 36750 077 365

3. HERAtray 1/4 Width, SS (3 pc.)HERAtray 1/4 Width, Cu (3 pc.)Width: 135 mm (Overall)Depth: 485 mm (Overall)Thickness: 1 mm

50 065 80750 065 808

4. HERAtray 1/3 Width, SS (3 pc.)HERAtray 1/3 Width, Cu (3 pc.)Width: 180 mm (Overall)Depth: 485 mm (Overall)Thickness: 1 mm

50 065 80550 065 806

5. Shelf for units with gas screen

Half width inc. support rails, SSHalf width inc. support rails, CuWidth: 260 mm (Overall)Depth: 500 mm (Overall)Thickness: 1 mm

50 065 79550 065 796

6. For units with gas screen

HERAtray 1/2 Width, SS (2 pc.)HERAtray 1/2 Width, Cu (2 pc.)Width: 125 mmDepth: 485 mmThickness: 1 mm

50 065 80950 065 810


Version: 07.2003 / 50078754 7


1. Plate for stacking 2 x HERAcell 240Width: 780 mmDepth: 772 mm

50 068 677

2. Plate for stacking a HERAcell 240with a BB 6220 or BBD 6220 orcytoperm 2 or B 5060 or B 5061Width: 895 mmDepth: 721 mm

Information:The cytoperm 2 or BBD 6220 shouldstand on the adaptor plate

50 066 094


Version: 07.2003 / 50078754 8

3 Accessories for cytoperm 2 / BBD 6220

3.1 Support frames

1. Support frame 300 mmWidth: 885 mmHeidht: 300 mmDepth: 645 mm

50 031 349


50 029 597

3. Stacking frame with castors forstacking 2 x BBD 6220 or cytoperm 2

Support frame for lower unit:Width: 920 mmHeight: 200 mmDepth: 670 mm

Support frame for upper unit:Width: 1080 mmHeight: 1215 mmDepth: 770 mm

Information:Alternatively another unit can bestacked in the upper position, e.g.BB6220 or HERAcell 240

50 053 628


Version: 07.2003 / 50078754 9

5. Castor set for 1 & 2 above. (Set of 4)

Information:When fitted the castors will raise thesupport frame by 90 mm.

50 052 528


Version: 07.2003 / 50078754 10


1. Shelf full width, SS, inc. support rails,Width: 560 mm (Overall)Depth: 500 mm (Overall)Thickness: 1 mm

50 029945

3. HERAtray 1/4 Width, SS (3 pc.)Width: 135 mm (Overall)Depth: 485 mm (Overall)Thickness: 1 mm

50 065 807

4. HERAtray 1/3 Width, SS (3 pc.)Width: 180 mm (Overall)Depth: 485 mm (Overall)Thickness: 1 mm

50 065 805

5. Shelf for units with gas screen

Half width, SS, inc. support railsWidth: 260 mm (Overall)Depth: 500 mm (Overall)Thickness: 1 mm

50 029 943

6. For units with gas screen

HERAtray 1/2 Width, SS (2 pc.)Width: 125 mmDepth: 485 mmThickness: 1 mm

50 065 809


Version: 07.2003 / 50078754 11


1. Plate to stack BBD 6220, cytoperm 2with a BB 6220 or HERAcell 240 orB 5060 or B 5061Width: 895 mmDepth: 721 mm

50 066 094


Version: 07.2003 / 50078754 12

Anschrift:Kendro Laboratory Products GmbHRobert – Bosch – Straße 1D - 63505 LangenselboldDeutschland

Telefon:Vertrieb/Service + 49 (0) 6184 / 906940

Fax:Vertrieb/Service + 49 (0) 6184 / 907474

Technical Information

Writer : R Bloomfield

Reviewed by : S CZWAK

Department

Tech Support

Cytoperm 2 & BBD 6220 030424 Page 1 of 1 Date: 24.04.2003

R Bloomfield 05/09/2005

Technical Information Cytoperm 2 & BBD 6220 030424 Problem: Poor reliability of circulating fan. Reason: The was only run at 32% speed, consequently any tightness in the bearings

would cause the fan to stop. Solution: Introduction of a new circulating fan with sped control and new software.

The kit will be supplied with full installation instructions in German and English.

Parts: 50076706 Retrofit Kit 50065196 Replacement fan Action: In the event of a fan motor failure in an existing unit, fit a retrofit kit. Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40258777 have been manufactured with the new fan.




Department

Tech Support



Technical Information Cytoperm 2 & BBD 6220 030425 Problem: Poor reliability of 03672272 evaporator oven. Reason: The heating element within the oven sometimes went open circuit due to

poor connection between the element and the outgoing connection wires. Solution: Introduction of a new evaporator oven, with a different heating element and

robust connections. Parts: 03672272 Evaporator Oven – the part number has not changed, the new

part will be supplied for all future orders. Action: In the event of a failure replace using the new oven. Information : The new oven can be identified by a ceramic terminal block on the right

hand side. The pictures below show new and old.

New




Department

Tech Support



Old Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40277650 have been manufactured with the new oven.




Department

Tech Support



Technical Information Cytoperm 2 & BBD 6220 030804 Problem: Contamination of the water tank and associated water tubing. Reason: Air or water borne contaminants would enter the water tank and grow

resulting in blockages in the water tubing valve and restrictor. Solution: Introduction of a new heated water tank. This tank is made from black

plastic and contains a heater that heats the water in the tank to 90 degrees every 2 hours.

Parts: 50076562 Water Tank Upgrade Kit

The kit includes full installation instructions. The installation instructions can also be found on the July 2003 release of the service CD ROM.

Action: In the event of a complaint or during a service visit offer the kit as a

chargeable upgrade. Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40307556 have been manufactured with the new heated water tank.




Department

Tech Support



Technical Information cytoperm 2 & BBD 6220 031020 Problem: Fan speed control non functional Reason: Wrong value resistor on the speed control board

(Pat No. 50056271) The voltage on terminal strip X2 pin 3 & 4 is above 2,8 V

Solution: Replace the existing 560 Ohm resistor R2 (see picture) with a 240 Ohm

resistor Parts: Resistor 240 Ohm, 0.25 W, 10% tolerance Action: After replacing the resistor, check the voltage on pin 3 & 4 of terminal strip

X2. The Voltage should be approx. 2,8 V In the event of a complaint or during a service visit replace this resistor

Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40326127 have been manufactured with the new resistor.




Department

Tech Support



Resistor R2 New: 240 Ohm

pin 3

pin 4

Terminal strip X2

picture 1




Department

Tech Support



PCB fan speed control

picture 2

Thermo Service & Support Service Bulletin

Product Line Equipment Bulletin Issue Date

Incubator - Heraeus Cytoperm 2 /

BBD 6220 0412-LSB-INC-004 21 December 2004

Author Reviewed By

R Bloomfield

S Czwak

New Software

PURPOSE:

To inform the field of a new software release for cytoperm 2 / BBD 6220

EQUIPMENT AFFECTED:

Cytoperm 2 and BBD 6220 All cytoperm 2 / BBD 6220 with serial number 40439668 and later are equipped with the new Software Version.

DESCRIPTION / INFORMATION: The new Software 50065109 – C / 09.12.2004 has following changes:

• Function Level 1 Sub Level 2 removed • Function Level 601 Sub Level 4 added loading of the default parameters after an

EPROM change • Optimisation of the mains zero crossing point for the fan speed controller • Error 400 (Relative Humidity low, current value < set value –5%) will be reset after the

current value reaches the error band again • The error band for temperature is now –1K and +2K of set point • The control variable for the heating is now limited to a maximum of 30% • The door heating is not active whilst the door is open • The evaporator oven temperature is 450°C, the starting temperature for humidifying is

420°C

ADDITIONAL INFORMATION:

The retrofit kit, including instructions, can be purchased from LSB under part number 50083483.




BBD 6220 0501-LSB-INC-005 5 January 2005

Author Reviewed By

R Bloomfield

S Czwak

Noisy Air Pump

PURPOSE:

To inform the field of a reported problem with the over humidity air pump. It has been reported that on some incubators the noise level from the humidity air pump, 50047060 (Y1), is unacceptable.

EQUIPMENT AFFECTED:

All Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION:

Over approximately the last year some air pumps have been found to be excessively noisy, this is particularly noticeable in quiet laboratories. From the date of this bulletin all new pumps supplied for production or as spare parts have been tested to ensure that the maximum sound level is below 65dB(A) (10 cm distant).

ADDITIONAL INFORMATION: If this problem is reported replace the humidity air pump with a new pump from current stocks.





Author Reviewed By

R Bloomfield

S Czwak

New Software

PURPOSE:

To inform the field of problems fixed with the new software release for cytoperm 2 / BBD 6220

EQUIPMENT AFFECTED:

Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION: The new Software 50065109 – C / 09.12.2004 has fixed the following problems:

• Fan running continuously at high speed after switch on. • Intermittent Error 400 related to the humidity recovery coming into the error band,

canceling the alarm delay and then falling back outside the band again. • Intermittent over temperature errors (Error 101) caused by repeated door openings

over a short time frame. Four changes have been made:

ADDITIONAL INFORMATION: If any of these problems have been reported update with the new software, 50065109. For further information relating to these software changes refer to Service bulletin 0412-LSB-INC-004 .





Author Reviewed By

R Bloomfield

S Czwak

Evaporator oven

PURPOSE:

To inform the field of a temporary change in the contents of the spare part 03672272 EVAPORATOR OVEN (REPLACEMENT) for cytoperm 2 / BBD 6220.

EQUIPMENT AFFECTED:

Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION: Due to a high failure rate of the heating elements in the evaporator oven, the working temperature of the oven has been changed to 450°C. For the rest of this year the evaporator oven (spare part number 03672272) will be delivered together with the latest Software Version (50065109 – C / 09.12.2004). Please fit the new Software when replacing the evaporator oven. With the new Software the evaporator oven temperature is 450°C, the start temperature for the humidity control is now 420°C.

ADDITIONAL INFORMATION: Please refer to the Software installation instruction, included in the shipment, when fitting the new Software. Please refer to Service Bulletin 0412-LSB-INC-004 for further changes in the Software.


Produktline Gerät Bulletin Datum

Inkubator - Heraeus Cytoperm 2 /

BBD 6220 0508-LSB-INC-013 30 August 2005

Autor Korrektur

F. Born

R. Bloomfield

New water tank with pump

PURPOSE: To inform the field of a new water tank with pump for cytoperm 2/BBD6220. This was developed to avoid contamination and blocking of the system because of contaminated water.

EQUIPMENT AFFECTED: cytoperm 2 und BBD 6220

DESCRIPTION / INFORMATION: The system includes filter, pump, water tank with thermostats and a new valve. Order number 50085388 RETROFIT KIT REPLACEMENT CANISTER SET NEW

ADDITIONAL INFORMATION: The new water tank with pump has been used in the production of all cytoperm 2 and BBD 6220 with a serial number 40491983 and higher. Instructions are included with the kit.


Produktline Gerät Bulletin Datum

Inkubator - Heraeus Cytoperm 2 /

BBD 6220 0508-LSB-INC-013 30 August 2005

Autor Korrektur

F. Born

R. Bloomfield

Part overview:

1a Water tank with heater 1b PCB with thermostat 2 Filling canister (new) 3 Installation instructions 4a Water valve 4b Mounting bracket with pump 5 Fixing screws for water tank 6 Quick fit connector

1a

2

3

4a

5

1b

6 4b

cytoperm & bbd 6220

Documents