68 Abstracts

#242

8.4

A COMPARATIVE ANALYSIS OF ANTI-GLOBULIN (KAPPA), FACS~ AND CONVENTIONAL CROSS-~3~TCIIES. M Fotino~ A Menonj E Olasot P Sahajpal~ and M Suthan- thiran~ Immunoganetics Laboratory, The Rogosin Institute~ New York~ NY.

In order to evaluate the relative sensitivity of anti-kappap FACS and conventional cross-matshes~ 40 kidney recipients with differential graft outcome were selected: 30 with functioning grafts (14 with ) 2 years of graft function and 16 with • I year of graft function) and 10 who rejected their grafts within 2 months. Conventional cross-matches and the majority (34) of FACS were performed prior to transplantation; anti-kappa, Amos 3 washes and few FACS (6 cases) ware done using frozen° thawed cells and serao The results of the cross-matches were:

Graft Number NIII(long) Amos (2W) Amos(3W) Anti-kappa FACS Function + - + - + - + - + .......................................................................

> 1 Yea 16 0 16 0 16 0 16 1 15 1 15 > 2 Years 14 0 14 0 14 0 14 I 13 I 13 < 2 MOS 10 0 10 0 10 0 10 0 10 I 9

Moreover, the antibody specificity analysis of the 2 cases with positive anti-kappa and FACS cross-matches revealed the presence of donor specific anti-HLA class I antibodies: anti-B8 (IgM) in one case and anti-BW62 (IgG) in the other.

Conclusion: (I) Anti-kappa and FACS cross-matches detect minimal amounts of HLA antibodies not recognized by the conventional cross- matches; (2) It appeors that such reduced amounts of antibodies do not promote graft rejection, since the two recipients with do,Lor-specific anti-HLA class I antibodies retained excellent graft function; (3) Early rejection of the grafts was not always predicted by anti-kappa or FACS cross-match results.

#243 IO.1

CULTURAL ORIGINS OF HAPLOTYPES IN ~4ERICANS AND CARIBBEANS OF BLACK AFRICAN PESCENT. ~ , C.A. Alperr E.J.Yunis and J.E. Holloway, The Center for Blood Research, Harvard Medical School, Boston, MA, Department of Pan-African Studies, California State University-Northrop

MHC haplotypes (HLA-A, B, complement and DR alleles) cluster by ethnic or cultural groups within races. Haplotypes of black African origin form distinct clusters which we e~plain on the basis of the interrelationship of linguistic groupings to genetic markers and cultural groups. In our families, 14/48 {29 %) have HLA-Bw42 haplotypes. HLA-A30, Bw42, FC(I,9O)0, DRwI8 (7/14) is restricted to southeastern U.S. kindreds, while other more hetero- geneous BLA-Bw42 haplotypes only occur in Caribbeans (eg. HLA-A30, Bw42, FC1O, DRwlS, BLA-A3Ù, Bw42, FC31, DRw8, HLA-A3, Bw42, SC31, DRw18 and HLA-A23, Bw42, SC31, DRw6). HLA-Bw52 and/or -Bw53, FC(1,90)0, DRw18 haplotypes are found in both groups.

The languages of West African cultural groups and the Bantu belong to the Niger-Congo language family. Central African Bantu were 30% of the southeastern U.S. slave imports. The remainder came from many west African cultural groups (eg. Mande, Wolof, Akan). Caribbean slaves were almost exclusively from west African cultural groups. We hypothesize that: I.) The HLA-A3O, Bw42 and FC(1,9O)0, -DRw18 fragments are from primordial haplotypes which antedate subdivision of the Niger-Congo language family; 2.) HLA- A30, Bw42, FC(1,90)0, DRw18 is a Central African Bantu haplotype; 3.) Other HLA-Bw42 haplotypes observed in Afro-Caribbeans arose during the early division between the Mande and the other the Niger-Congo groups; and 4.) BLA-Bw52/Bw53, FC(1,90)0, DRw18 haplotypes are from Non-Mande west African cultural groups.