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DEPARTMENT OF BIOTECHNOLOGY (U.G.)

GOVERNMENT NAGARJUN P.G. COLLEGE OF SCIENCE

RAIPUR(C.G.)-492010

Course outcome

B.Sc. Part-I

CO1-Biochemistry- This course presents all the chemical reactions & metabolic processes

taking place in the living system. It also deals with the mechanism of regulation of all these

processes. After successful completion of this course the student will understand basic structure,

classification & function of biomolecules & their metabolic pathways.

CO2-Biostatistics & Computers-This course includes basic concepts of Bostatistics &

computers. This will enable students to understand the statistical aspects of various techniques.

The knowledge of biostatistics & computers will help in understanding the methods of

organizing and analysing biological data.

CO3-Cell Biology- This course provides an insight into the world of basic unit of life, the cell,

after completion of this course, the student will understand the basic organization & function of

cell & its components.

CO4-Genetics- In this course, classical Mendelian genetics & its various concepts are

included.It also includes various genetic anomalies & abberations

CO5-Microbiology- This course includes study of micro-organisms and their different

biological processes.It also presents the industril aspect of microbiology.

Practical- Basic Microbiological & biochemical techniques like media preparation, Staining

methods, culture and estimation of biomolecules.

B.Sc. Part-II

CO6-Molecular Biology- In this course the student will understand the molecular basis

of biological activity , biosynthesis , & interactions between biomolecules in the various systems

of a cell.

CO7-Biophysics- The objective of this course is to make students understand the theories and

methods of physics that explain how biological systems work.

CO8-Recombinant DNA Technology- The objective of this course is to make students

understand the technique of inserting foreign DNA into an organism to produce desirable traits.

Practical- Isolation of DNA, RNA & biotechniques like chromatography, electrophoresis,

centrifugation.

B.Sc. Part-III

CO9-Plant Biotechnology- The objective of this course is to make students understand modern

plant biotechnological processes like tissue culture & genetic engineering for production of

improved plant varieties.

CO10-Environmental biotechnology- This course includes basic Environmental issues & their

CO11-Industrial Biotechnology- In this course, students get the basic knowledge of

bioreactors& various industrial processes

CO12-IMMUNOLOGY- Students will gain knowledge on how the immune system works and

also on the immune system network and interactions during a disease or pathogen invasion.

Practical-Plant tissue culture, environmental assessment techniques, & immunological

techniques



RAIPUR(C.G.)-492010

SCHEME OF EXAM

SESSION -2019-2020

B.Sc. PART I- BIOTECHNOLOGY

(A) THEORY

PAPER TITLE MM

I Biochemistry, Biostatistics & Computer

Paper code-1007

50

II Cell Biology, Genetics & Microbiology paper

code-1008

50

(B) PRACTICAL

Practical Exam paper code-1009 50

Total 150

B.Sc. Part I

Session-2019-20

Subject- BIOTECHNOLOGY

Paper I

Biochemistry, Maths & Computer

MM 50

Unit I

1. Introduction to Biochemistry: History, scope & development.

2. Carbohydrates: Classification, Structure & function of mono, oligo & polysaccharides.

3. Lipids: Structure, classification & Function.

UnitII

1. Amino acids & Proteins: Classification ,Structure & Proprties of Amino acids, Types of

Proteins & their Classification and Function.

2. Enzymes: Nomenclature & Classification of enzyme. Mechanism of enzyme action ,

enzyme kinetics & factors affecting the enzyme action. Immobilization of enzyme &

their Application.

Unit III

1. Hormones: Plant hormone- Auxin & Gibberelins and Animal hormone- Pancreas &

thyroid.

2. Carbohydrate, Protein & Lipid metabolism- Glycolysis, Glycogenesis, Glyconeogenesis,

Glycogenolysis & Krebs cycle. Electron transport chain & β-oxidation of fatty acids

Unit IV

1. Scope of Biostatistics, Samples & population concept,Collection of data sampling

techniques, Processing and presentation of Data

2. Measurements of Central tendencies – Mean, Median ,Mode & Standard deviations.

3. Probability calculation: Definition of Probability, Theorem on total and Compound

Probability

Unit V

1. Computers: General introduction, organization of computer, digital & analog computers,

computer algorithm.

2. Cocept of hardware & Software, Input And Output device

3. Application of computer in co-ordination of solute concentration, pH & temperature etc.

of a fermenter in operation & Internet Application.

List of Books:

1. Nelson & Cox(2005) Principles of Biochemistry, Fourth Edition.

2. Todd & Howards Mason (2004) Textbook of Biochemistry, Fourth Edition.

3. Lubert Stryer & Berg (2004) Biochemistry, Fifth Edition.

4. Diana Rain, Marni Ayers Barby(2006) Textbook on Q level Programming, 4th

Edition.

5. K. Schwartz (2006) Guide Of Microsoft, 4th

Edition

6. E. Balaguruswamy (1991) Programming in BASICS.

7. R. C. Campbell Statistics for Biologists.

8. P Cassel –Inside Microsoft Office.

9. G.W. Snedecor and W.G.Cochran –Statistical methods.

10. Practical Statistics for Experimental Biologists by A.C. Wardlaw

11. Biostatistical Analysis by J.H. zar.

12. Introduction to Biostatistics by R.r. Sokal and F.J. Rohlf.

13. L.Y. Kun (2003) Microbial Biotechnology: Principles and applications

14. Fundamental of Biostatistics by Khan and Khanum (1994)

15. Albert Lehninger: Biochemistry (2004).

16. Diana rain & Barby : Textbook on Q Level Programming (2006).

Pattern of Questions paper Section Type of Questions Word Limit No. of Questions Mark in

each

Questions

Marks in

each

Section

Section A Objective type/ MCQ - 10 (Two questions from each unit) 01 10

Section B Short Answer type 75-100 Words 5 (Two questions from each unit with internal choice) 03 15

Section C Long Answer type 250-300 Words 5 (Two questions from each unit with internal choice) 05 25

Total Marks 50

Members of Board of Studies in Biotechnology

Chair person- Dr. Sanjana Bhagat Signature

Sub. Expert- Dr S.K.Jadhav

Sub. Expert- Dr Anil Kumar Shrivastava

Sub. Expert- Dr Keshav Kant Kahu

Industrialist – Mr. Kaustubh Dharmadhikari

Deptt.member— Mrs. Deepali Rajwade

Student Nominee- Ms. Durga Verma

B.Sc. Part I

Session-2019-20


Paper II

Cell Biology, Genetics & Microbiology

MM 50

Unit I

1. Concept of Life, Cell as a basic unit of living system and cell theory.

2. Diversity of cell shape and size.

3. Prokaryotic cell structure: Functionand ultra structure of cell ( Gram positive and Gram

negative bacteria), Plasma membrane ,flagella, Pilli, Endospore and Capsule.

4. Eukaryotic cell: Plant Cell wall & plasma membrane

Unit II

1. Cytoplasm: Structure and function of Endoplasmic Reticulum, Ribosome , Golgi

complex, Lysosomes, Nucleus, Mitochondria and Chloroplast

2. Cytoskeleton: Microtubules, Microfilaments and Intermediate filaments.

3. Cell division : Mitosis and Meiosis

4. Programmed Cell Death

Unit III

1. Mendel’s Laws of inheritance.

2. Linkage & Crossing Over.

3. Chromosome variation in number and structure.: deletion, duplication, translocation,

inversion and Aneuploidy, euploidy ( monoploidy & polyploidy & its importance).

Unit IV

1. History ,scope and development of Microbiology

2. Basic techniques of microbial culture

3. Microbial growth & nutrition of Bacteria: Isolation, media sterilization – physical and

chemical agents, pure culture –pour plate method, streak plate method and spread plate

method.

4. General features and Economic importance of Fungi, Algae and Protozoa etc.

Unit V

1. Bacterial Reproduction: Conjugation, transduction & transformation.

2. Mycoplasma: History, classification, structure, reproduction & diseases.

3. Viruses: Basic features, structure, classification, multliplication, bacteriophage (

morphology, life cycle, infection, medicinal importance).

List of Books:

1. C. B. Powar: Cell Biology , 1st Edition (2005), Himalaya Publishing House.

2. Gerald Karp: Cell & Molecular Biology(2005),4th

Edition

3. P.K. Gupta: Cell & Molecular Biology (2003),2nd

Edition Rastogi Publications.

4. S.S.Purohit –Microbiology: Fundamentals and Applications ,6th

Edition (2004)

5. R.C.Dubey and D.K.Maheshwari: Practical Microbiology . S. Chand Publication

6. R.C.Dubey and D.K.Maheshwari: (2006)Microbiology S. Chand Publication

7. Tortora , Funke and Case- Microbiology- An Introduction, 6th

Edition(1995),Benjamin Cummings Publishing Company.

8. Prescott, Harley and Klein- Microbiology, 3rd

Edition(1996)

9. P. Chakraborthy-Textbook of Microbiology, 2nd

Edition (2007)

10. Microbial Genetics, David Freifelder

11. S.K.Jadhav and P.K.Mahish(2018) Prayogatmak Jaivprodyogiki awam

Sukshmjivigyan chattisgarh Hindi Granth Academy , Raipur


each

Questions

Marks in

each

Section




Total Marks 50









B.Sc. Part I

Session-2019-20


Practical List

MICROBIOLOGY AND BIOCHEMICAL TECHNIQUES

1. Laboratory rules, tools, equipment & other requirements in microbiological

laboratory.

2. Micrometry: Use of oculer & stage micrometer.

3. Counting of bacteria by counting chamber, by plate count.

4. Preparation of media & cultivation techniques

a)Basic liquid media(broth)

b)basic solid media(agar slants and deep tubes)

c) Demonstration of selective and differential media

d) Isolation and enumeration of microorganisms

e) Isolation from air and soil.

5. Smears & staining methods

a) Preparation of bacterial smear

b) Negative & positive gram staining

6. Methods of obtaining pure culture

a) Streak plate method

b) Pour plate method

c) Spread plate method

d) Broth culture

7. Growth & Biochemical Techniques

a) Determination of bacterial growth curve

b) Amylase production test

c) Cellulose production test

d) Estimation of sugar in given solution

e) Extraction & seperation of lipids

f) Estimation of protein

g) Mitosis and meiosis

8. Biostatistics

a)By manual & by computer

b) Problems on mean, mode & median

.

Scheme for Practical Examination

Time: 4 hrs MM: 50

1. .Experiment based on culture of micro-organisms 15

2. Bacterial growth and staining techniques 10

3. Biochemical techniques 05

4. Biostatistics 05

5. Spotting 05

6. Viva voce 05

7. Record/ Session 05











RAIPUR(C.G.)-492010

SCHEME OF EXAM

SESSION-2019-20

B.Sc. PART II- BIOTECHNOLOGY

(A) THEORY

PAPER TITLE MM

I Molecular Biology & Biophysics Paper

code- 2006

50

II Recombinant DNA Technology Paper

code- 2007

50

(B) PRACTICAL

Practical Exam Paper code- 2008 50

Total 150

B.Sc. Part II

Session-2019-20


Paper I

Molecular Biology & Biophysics.

MM 50

Unit I

1. DNA: Structure , types & replication

2. RNA; Structure, type & function

3. Structure of Gene- Old & New concept

Unit II

1. Genetic Code: Properties, codon assignment, secondary genetic code

2. Protein synthesis

3. Mitochondrial genome

4. Chloroplast genome

Unit III

1. Gene therapy

2. Transposable elements

3. DNA damage & repair

4. Tissue Engineering : General concept

Unit IV

1. Law of thermodynamics

2. Beer Lambart law

3. Radioisotopes rechniques

4. Autoradiography

Unit V

1. Biophysics- Introduction, scope & application

2. Principle, structure & functions of following-

a) Spectroscopy

b) Electrophoresis

c) Centrifugation

d) Colorimeter

e) Chromatography

f) ELISA

List of Books:

1. C.B. Pwar: Cell Biology, Himalay Publishing House

2. Gerald Karp: Cell & Molecular Biology(2005)

3. Lewis, Smith& Valerie: Principles of Cell & Molecular Biology(2002)

4. P.K. Gupta: Cell & Molecular Biology (2003), Rastogi Publications.

5. R. Twyaman: Advanced Molecular Biology (1998)

6. Wilson & Walker: Principle & Techniques of Biotechnology & molecular

biotechnology.

7. Upadhya & Upadhya:Biophysical Chemistry.


each

Questions

Marks in

each

Section




Total Marks 50

B.Sc. Part II

Session-2019-20


Paper II

Recombinant DNA Technology

MM 50

Unit I

1. Scope & aim of Biotechnology

2. Recombinant DNA Technology : General Concept & application, Strategies of

recombinant DNA Technology in Prokaryotes.

3. Restriction enzymes: endonucleases( type, nomenclature, restriction sequences &

cleavage pattern)

a) Modification of cut ends

b) Steps in gene cloning

c) Isolation of desired gene

4. cDNA library , genomic library

Unit II

1. vectors( animal & plant vectors)

2. Bacteriophage vectors

3. Introduction of vectors into appropriate host

Unit III

1. PCR: Procedure (denaturation , annealing, extension)

2. Types of PCR

3. Applications, advantages & limitations of PCR

Unit IV

1. Monoclonal antibodies: Structure , production & application

2. In vitro fertilization & embryo transfer

3. Genome map & genome project

4. Apoptosis

Unit V

1. Stem cell technology

2. Targeted gene transfer

3. DNA fingerprinting

4. Transgenic animals & plants

List of books

1. B.D. singh : Biotechnology, Expanding Horizons. Kalyani Publishers

2. P.K. Gupta: Biotechnology & genomics(2003), Rastogi Publications

3. Patel: Industrial Microbiology(2003)

4. Satyanarayan: Biotechnology(2005)

5. Bilgrami & fandey: introduction of Biotechnology(1998)


each

Questions

Marks in

each

Section




Total Marks 50







B.Sc. Part II

Session-2019-20


Practical List

1. Isolation of DNA

2. Isolation of RNA

3. Estimation of DNA from Plant cells

4. Laminar flow,autoclave,oven, incubator, water bath, Quebec Colony counter,

centrifuge, spectrophotometer, electrophoresis, camera lucida

5. Experiments (at least two) based on electrophoresis.

Scheme of Practical examination

Time: 4 hrs MM: 50

1. DNA isolation 10

2. RNA isolation 10

3. Practical based on Biophysics 10

4. Spotting based on Paper I & II 10

5. Viva voce 05

6. Record/ Sessional 05









DEPARTMENT OF BIOTECHNOLOGY(U.G.)


RAIPUR(C.G.)-492010

SCHEME OF EXAM

SESSION-2019-20

B.Sc. PARRT III- BIOTECHNOLOGY

(A) THEORY

PAPER TITLE MM

I General Biotechnology: Plant, Environment and

Industrial Biotechnology paper code-3006

50

II Immunology paper code-3007 50

(B) PRACTICAL

Practical Exam paper code-3008 50

Total 150

B.Sc. Part III

Session-2019-20


Paper I

Plant, Environment and Industrial Biotechnology

MM 50

Unit I

1. Plant cell & tissue culture: General introduction, history & scope.

2. Application of tissue culture

3. Concept of cellular differentiation

4. Agrobacterium Ti & Ri plasmid

5. Bt gene, molecular marker(RFLP,RAPD), edible vaccines

Unit II

1. Organogenesis, embryogenesis, protoplast isolation & fusion

2. Germplasm storage & cryopreservation

3. Anther & ovary culture

Unit III

1. General introduction & scope of Environmental biotechnology

2. Environmental pollution & its types

3. Control of Pollution through biotechnology

4. Wastewater treatment : Physical, Chemical, biological

Unit IV

1. Biofertilizer, biopesticides, IPR

2. Global environmental problems: general introduction, ozone depletion, acid rain, green

house effect.

Unit V

1. Bioreactor & its types

2. Fermentation (lactic acid, alcohol)

3. Maintenance of Industrial microorganisms

4. Food technology: Introduction, canning, packing, & food preservation.

Books:

1. Textbook of Biotechnology: Indu shekhar Thakur- I.K. International Pvt Ltd. New Delhi

2. Biotechnology( Fundamentals & Applications): S.S. Purohit- Agrobios ( India) Jodhpur

3. Fundamentals of Microbiology & Immunology: Ajit K. Bannerjee, Nirmalya Bannerjee

New central book agency(P) Ltd. Kolkata

4. Plant Biotechnology : R.S. Chawla- Oxford & IBH Publishing Co. Pvt. Ltd. New delhi

5. Plant Biotechnology: B.D.Singh Kalyani Publication, New Delhi

6. Biotechnology: Fundamentals & applications: S.s. Purohit

7. Immunology: Roitt et al

8. Plant tissue culture practical ; H.S. Chawla


each

Questions

Marks in

each

Section




Total Marks 50

B.Sc. Part III

Session-2019-20


Paper II

Immunology

MM 50

Unit I

Immunology: General concept, history & development

Immune system & immunity, organization of immune system

Antigen- antibody & its types

Unit II

Cells involved in immune system, types & cells. Basic structure & function .

Cytokines

Cell mediated immunity, Interferons, hypersensitivity

Unit III

Antigen- antibody interaction: principles & types

Immunohematology: General concept, blood group system, Rh factor, medical application of blood

groups

Unit IV

Origin & diversity in immune system

Effector mechanism

Immunity to infectious diseases, monoclonal antibodies

Unit V

Autoimmune diseases, hemolytic anaemia, rheumatoid arthritis, insuin dependent diabetes, myasthenis

gravis. Organ transplantation

Immunodeficient diseases. Cancer, AIDS

List of Books

1. Thakur: A textbook of biotechnology

2. Purohit: Biotechnology

3. Chawla:Plant Biotechnology

4. Kubey: Immunology

5. Paul: Fundamental of Immunology

6. Razdan: Plant Tissue Culture


each

Questions

Marks in

each

Section




Total Marks 50









B.Sc. Part III

Session-2019-20


Practical List

Plant:

1. Sterilization of plant material

2. Preparation of tissue culture media

3. Plant tissue culture by plant parts

Environment:

1. Determination of total dissolved solids of water

2. Determination of DO,BOD,COD of water

3. MPN test

Industrial:

1. Food preservation techniques

2. Application of biopesticides on microorganisms

3. Production of citric acid by microorganisms

Immunology

1. Blood grouping in relation to antigen antibody interaction

2. Rh factor determination

3. Widal test

4. VDRL test

5. Double diffusion experiment

6. ELISA test

Scheme for practical examination

Time:4hrs MM:50

1. Experiment based on Paper I

i) Plant tissue culture 08

ii) Environment/ industrial 07

2. Practical based on paper II 15

3. Spots 05 (based on paper I & II) 10

4. Viva voce 05

5. Record/ Sessional 05









Course Outcomes

B.Sc. Biotechnology

B.Sc. I

Course Outcome

CO1-Biochemistry

This course presents all the chemical reactions & metabolic processes taking place in the living system. It also deals with the mechanism of regulation of all these processes. After successful completion of this course the student will understand basic structure, classification & function of biomolecules & their metabolic pathways. B.Sc.Paper I-Unit I,II,III

CO2-Biostatistics & Computers This course includes basic concepts of bBostatistics & computers. This will enable students to understand the statistical aspects of various techniques. The knowledge of biostatistics & computers will help in understanding the methods of organizing and analyzing biological data. B.Sc.I –PaperI- Unit-IV & V

CO3-Cell Biology This course provides an insight into the world of basic unit of life, the cell.after completion of this course,the student will understand the basic organization & function of cell & its components. B.Sc.I –PaperII- Unit-I & II

CO4-Genetics In this course,classical Mendelian genetics & its various concepts are included.It also includes various genetic anomalies & abberations B.Sc.I –PaperII- Unit-II & III

CO5-Microbiology This course includes study of micro-organisms and their different biological processes.It also presents the industril

aspect of microbiology. B.Sc.I –PaperII- Unit-IV & V

Practical Basic Microbiological & biochemical techniques like media preparation, Staining methods, culture and estimation of

biomolecules. B.Sc. II

CO6-MOLECULAR BIOLOGY In this course the student will understand the molecular basis

of biological activity , biosynthesis , & interactions between

biomolecules in the various systems of a cell

PAPER - I Unit-I , II& III

CO7-BIOPHYSICS The objective of this course is to make students understand

the theories and methods of physics that explain how biological

systems work

PaperI- Unit-IV & V

CO8-RECOMBINANT DNA The objective of this course is to make students understand

TECHNOLOGY the technique of inserting foreign DNA into an organism to produce

desirable traits.

PaperII- Unit-I,II,III,IV,V

Practical Isolation of DNA ,RNA & biotechniques like chromatography,

electrophoresis,centrifugation.

B.Sc. III

CO9-Plant Biotechnology The objective of this course is to make students understand modern plant biotechnological processes like tissue culture

&genetic engineering for production of improved plant varieties.

B.Sc.III –PaperI- Unit-I & II CO10-Environmental biotechnology This course includes basic Environmental issues & their

management. CO11-Industrial Biotechnology In this course,students get the basic knowledge of

bioreactors& various industrial processes CO12-IMMUNOLOGY Students will gain knowledge on how the immune system

works and also on the immune system network and interactions during a disease or pathogen invasion.

Practical Plant tissue culture, environmental assessment techniques, & immunological techniques

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