correction: structural and functional properties of ribosomes crosslinked with dimethyl-suberimidate
TRANSCRIPT
Correction: Structural and Functional Properties of Ribosomes Crosslinked with Dimethyl-suberimidateSource: Proceedings of the National Academy of Sciences of the United States of America,Vol. 70, No. 5 (May, 1973), p. 1618Published by: National Academy of SciencesStable URL: http://www.jstor.org/stable/62177 .
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1618 Biochemistry: Wickner et al.
The complementation assay, as described, above, is also being used to purify the dna B gene product. Partially purified fractions, isolated from dna B ts cells, are thermolabile compared to fractions isolated from dna C ts cells. Also by this complementation assay, activities that stimulate dna C and dna D receptor crude extracts have been purified. Thus, by complementation in the sX174 DNA-dependent system, it should be possible to purify the products of all the known dna genes. Because a defined DNA template is used, it should be possible to analyze the mechanism of DNA synthesis carried out by this system.
This work was supported by grants from the National Institute of Health (GM-13344) and the American Cancer Society (NP- 8900). S. W. is a Predoctoral trainee of The National Institute of Health, while M. W. is a Fellow of The Jane Coffin Childs Memo- rial Fund for Medical Research.
1. Hirota, Y., Ryter, A. & Jacob, F. (1968) Cold Spring Harbor Symp. Quant. Biol. 33, 677-693.
2. Wechsler, J. A. & Gross, J. D. (1971) Mol. Gen. Genet. 113, 273-284.
3. Carl, P. L. (1970) Mol. Gen. Genet. 109, 107-122. 4. Gefter, M. L., Hirota, Y., Kornberg, T., Wechsler, J. &
Barnoux, C. (1971) Proc. Nat. Acad. Sci. USA 68, 3150- 3153.
5. Niisslein, V., Otto, B., Bonhoeffer, F. & Schaller, H. (1971) Nature New Biol. 234, 285-286.
6. Fuchs, J. A., Karlstom, H. O., Warner, H. R. & Reichard, P. (1972) Nature New Biol. 238, 69-71.
Correction. In the article "Structural and Functional
Properties of Ribosomes Crosslinked with Dimethyl-
suberimidate," by Slobin, L. I., which appeared in the
December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3769-3773, p. 3770, right-hand column, line 3 from top, 10 mM KCI-6 mM 2-mercaptoethanol (pH 8.5) should read: 100 mM KCI-6 mM 2-mercaptoethanol (pH 8.5).
1618 Biochemistry: Wickner et al.
The complementation assay, as described, above, is also being used to purify the dna B gene product. Partially purified fractions, isolated from dna B ts cells, are thermolabile compared to fractions isolated from dna C ts cells. Also by this complementation assay, activities that stimulate dna C and dna D receptor crude extracts have been purified. Thus, by complementation in the sX174 DNA-dependent system, it should be possible to purify the products of all the known dna genes. Because a defined DNA template is used, it should be possible to analyze the mechanism of DNA synthesis carried out by this system.
This work was supported by grants from the National Institute of Health (GM-13344) and the American Cancer Society (NP- 8900). S. W. is a Predoctoral trainee of The National Institute of Health, while M. W. is a Fellow of The Jane Coffin Childs Memo- rial Fund for Medical Research.
1. Hirota, Y., Ryter, A. & Jacob, F. (1968) Cold Spring Harbor Symp. Quant. Biol. 33, 677-693.
2. Wechsler, J. A. & Gross, J. D. (1971) Mol. Gen. Genet. 113, 273-284.
3. Carl, P. L. (1970) Mol. Gen. Genet. 109, 107-122. 4. Gefter, M. L., Hirota, Y., Kornberg, T., Wechsler, J. &
Barnoux, C. (1971) Proc. Nat. Acad. Sci. USA 68, 3150- 3153.
5. Niisslein, V., Otto, B., Bonhoeffer, F. & Schaller, H. (1971) Nature New Biol. 234, 285-286.
6. Fuchs, J. A., Karlstom, H. O., Warner, H. R. & Reichard, P. (1972) Nature New Biol. 238, 69-71.
Correction. In the article "Structural and Functional
Properties of Ribosomes Crosslinked with Dimethyl-
suberimidate," by Slobin, L. I., which appeared in the
December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3769-3773, p. 3770, right-hand column, line 3 from top, 10 mM KCI-6 mM 2-mercaptoethanol (pH 8.5) should read: 100 mM KCI-6 mM 2-mercaptoethanol (pH 8.5).
1618 Biochemistry: Wickner et al.
The complementation assay, as described, above, is also being used to purify the dna B gene product. Partially purified fractions, isolated from dna B ts cells, are thermolabile compared to fractions isolated from dna C ts cells. Also by this complementation assay, activities that stimulate dna C and dna D receptor crude extracts have been purified. Thus, by complementation in the sX174 DNA-dependent system, it should be possible to purify the products of all the known dna genes. Because a defined DNA template is used, it should be possible to analyze the mechanism of DNA synthesis carried out by this system.
This work was supported by grants from the National Institute of Health (GM-13344) and the American Cancer Society (NP- 8900). S. W. is a Predoctoral trainee of The National Institute of Health, while M. W. is a Fellow of The Jane Coffin Childs Memo- rial Fund for Medical Research.
1. Hirota, Y., Ryter, A. & Jacob, F. (1968) Cold Spring Harbor Symp. Quant. Biol. 33, 677-693.
2. Wechsler, J. A. & Gross, J. D. (1971) Mol. Gen. Genet. 113, 273-284.
3. Carl, P. L. (1970) Mol. Gen. Genet. 109, 107-122. 4. Gefter, M. L., Hirota, Y., Kornberg, T., Wechsler, J. &
Barnoux, C. (1971) Proc. Nat. Acad. Sci. USA 68, 3150- 3153.
5. Niisslein, V., Otto, B., Bonhoeffer, F. & Schaller, H. (1971) Nature New Biol. 234, 285-286.
6. Fuchs, J. A., Karlstom, H. O., Warner, H. R. & Reichard, P. (1972) Nature New Biol. 238, 69-71.
Correction. In the article "Structural and Functional
Properties of Ribosomes Crosslinked with Dimethyl-
suberimidate," by Slobin, L. I., which appeared in the
December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3769-3773, p. 3770, right-hand column, line 3 from top, 10 mM KCI-6 mM 2-mercaptoethanol (pH 8.5) should read: 100 mM KCI-6 mM 2-mercaptoethanol (pH 8.5).
Proc. Nat. Acad. Sci. USA 70 (1973)
7. Moses, R. & Richardson, C. C. (1970) Proc. Nat. Acad. Sci. USA 67, 674-681.
8. Schaller, H., Otto, B., Nuisslein, V., Huf, J., Herrmann, R. & Bonhoeffer, F. (1.972) J. Mol. Biol. 63, 183--200.
9. Wickner, R. B. & Hurwitz, J. (1972) Biochem. Biophys. Res. Commun. 47, 202-211.
10. Wickner, W. T., Brutlag, D., Schekman, R. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 965-969.
11. Schekmnan, R., Wickner, W. T., Westergaard, O., Brutlag, D., Geider, K., Bertch, L. L. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 2691-2695.
12. Wickner, R. B., Wright, M., Wickner, S. & IHurwitz, J. (1972) Proc. Nat. Acad. Sci. USA 69, 3233-3237.
13. Nuisslein, V., Bonhoeffer, F., Klein, A. & Otto, B. (1972) in The Second Annual Harry Steenbock Symposium, eds. Wells, R. & Inman, R. (University Park Press, Maryland), in press.
14. Campbell, J. L., Soll, L. & Richardson, C. C. (1972) Proc. Nat. Acad. Sci. USA 69, 2090-2094.
15. Sinsheimer, R. L. (1966) in Procedures in Nucleic Acid Research, eds. Cantoni, G. L. & Davies, D. R. (Harper and Row, New York), pp. 569-576.
16. Franke, B. & Ray, D. S. (1970) Virology 44, 168-187. 17. Biucher, T. (1947) Biochim. Biophys. Acta, 1, 292-314. 18. Schaller, H., Nusslein, C., Bonhoeffer, F. J., Kurz, C. &
Neitzschmann, I. (1972) Eur. J. Biochem. 26, 474-481. 19. Sigal, N., Delius, H., Kornberg, T., Gefter, M. L. & Alberts,
B. (1972) Proc. Nat. Acad. Sci. USA 69, 3537-3541. 20. Jones, 0. W. & Berg, P. (1966) J. Mol. Biol. 22, 199-209. 21. Wickner, R. B., Ginsberg, B., Berkower, 1. & Hurwitz, J.
(1972) J. Biol. Chem. 247, 489-497. 22. Hurwitz, J., Wickner, S. & Wright, M. (1973) Biochem.
Biophys. Res. Commun., 51, 257-267.
Correction. In the article "Assembly of Rod-Shaped Virus In Vitro: Reconstitution with Cucumber Green Mottle Mosaic Virus Protein and Tobacco Mosaic Virus RNA," by Ohno, T., Inoue, H. & Okada, Y., which appeared in the December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3680-3683, p. 3861, right-hand column, the first line should read: "as 13 S, Fig. 2B); whereas. . . "; the fifth line should read: "(Fig. 2A). The results. . .".
Proc. Nat. Acad. Sci. USA 70 (1973)
7. Moses, R. & Richardson, C. C. (1970) Proc. Nat. Acad. Sci. USA 67, 674-681.
8. Schaller, H., Otto, B., Nuisslein, V., Huf, J., Herrmann, R. & Bonhoeffer, F. (1.972) J. Mol. Biol. 63, 183--200.
9. Wickner, R. B. & Hurwitz, J. (1972) Biochem. Biophys. Res. Commun. 47, 202-211.
10. Wickner, W. T., Brutlag, D., Schekman, R. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 965-969.
11. Schekmnan, R., Wickner, W. T., Westergaard, O., Brutlag, D., Geider, K., Bertch, L. L. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 2691-2695.
12. Wickner, R. B., Wright, M., Wickner, S. & IHurwitz, J. (1972) Proc. Nat. Acad. Sci. USA 69, 3233-3237.
13. Nuisslein, V., Bonhoeffer, F., Klein, A. & Otto, B. (1972) in The Second Annual Harry Steenbock Symposium, eds. Wells, R. & Inman, R. (University Park Press, Maryland), in press.
14. Campbell, J. L., Soll, L. & Richardson, C. C. (1972) Proc. Nat. Acad. Sci. USA 69, 2090-2094.
15. Sinsheimer, R. L. (1966) in Procedures in Nucleic Acid Research, eds. Cantoni, G. L. & Davies, D. R. (Harper and Row, New York), pp. 569-576.
16. Franke, B. & Ray, D. S. (1970) Virology 44, 168-187. 17. Biucher, T. (1947) Biochim. Biophys. Acta, 1, 292-314. 18. Schaller, H., Nusslein, C., Bonhoeffer, F. J., Kurz, C. &
Neitzschmann, I. (1972) Eur. J. Biochem. 26, 474-481. 19. Sigal, N., Delius, H., Kornberg, T., Gefter, M. L. & Alberts,
B. (1972) Proc. Nat. Acad. Sci. USA 69, 3537-3541. 20. Jones, 0. W. & Berg, P. (1966) J. Mol. Biol. 22, 199-209. 21. Wickner, R. B., Ginsberg, B., Berkower, 1. & Hurwitz, J.
(1972) J. Biol. Chem. 247, 489-497. 22. Hurwitz, J., Wickner, S. & Wright, M. (1973) Biochem.
Biophys. Res. Commun., 51, 257-267.
Correction. In the article "Assembly of Rod-Shaped Virus In Vitro: Reconstitution with Cucumber Green Mottle Mosaic Virus Protein and Tobacco Mosaic Virus RNA," by Ohno, T., Inoue, H. & Okada, Y., which appeared in the December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3680-3683, p. 3861, right-hand column, the first line should read: "as 13 S, Fig. 2B); whereas. . . "; the fifth line should read: "(Fig. 2A). The results. . .".
Proc. Nat. Acad. Sci. USA 70 (1973)
7. Moses, R. & Richardson, C. C. (1970) Proc. Nat. Acad. Sci. USA 67, 674-681.
8. Schaller, H., Otto, B., Nuisslein, V., Huf, J., Herrmann, R. & Bonhoeffer, F. (1.972) J. Mol. Biol. 63, 183--200.
9. Wickner, R. B. & Hurwitz, J. (1972) Biochem. Biophys. Res. Commun. 47, 202-211.
10. Wickner, W. T., Brutlag, D., Schekman, R. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 965-969.
11. Schekmnan, R., Wickner, W. T., Westergaard, O., Brutlag, D., Geider, K., Bertch, L. L. & Kornberg, A. (1972) Proc. Nat. Acad. Sci. USA 69, 2691-2695.
12. Wickner, R. B., Wright, M., Wickner, S. & IHurwitz, J. (1972) Proc. Nat. Acad. Sci. USA 69, 3233-3237.
13. Nuisslein, V., Bonhoeffer, F., Klein, A. & Otto, B. (1972) in The Second Annual Harry Steenbock Symposium, eds. Wells, R. & Inman, R. (University Park Press, Maryland), in press.
14. Campbell, J. L., Soll, L. & Richardson, C. C. (1972) Proc. Nat. Acad. Sci. USA 69, 2090-2094.
15. Sinsheimer, R. L. (1966) in Procedures in Nucleic Acid Research, eds. Cantoni, G. L. & Davies, D. R. (Harper and Row, New York), pp. 569-576.
16. Franke, B. & Ray, D. S. (1970) Virology 44, 168-187. 17. Biucher, T. (1947) Biochim. Biophys. Acta, 1, 292-314. 18. Schaller, H., Nusslein, C., Bonhoeffer, F. J., Kurz, C. &
Neitzschmann, I. (1972) Eur. J. Biochem. 26, 474-481. 19. Sigal, N., Delius, H., Kornberg, T., Gefter, M. L. & Alberts,
B. (1972) Proc. Nat. Acad. Sci. USA 69, 3537-3541. 20. Jones, 0. W. & Berg, P. (1966) J. Mol. Biol. 22, 199-209. 21. Wickner, R. B., Ginsberg, B., Berkower, 1. & Hurwitz, J.
(1972) J. Biol. Chem. 247, 489-497. 22. Hurwitz, J., Wickner, S. & Wright, M. (1973) Biochem.
Biophys. Res. Commun., 51, 257-267.
Correction. In the article "Assembly of Rod-Shaped Virus In Vitro: Reconstitution with Cucumber Green Mottle Mosaic Virus Protein and Tobacco Mosaic Virus RNA," by Ohno, T., Inoue, H. & Okada, Y., which appeared in the December 1972 issue of Proc. Nat. Acad. Sci. USA 69, 3680-3683, p. 3861, right-hand column, the first line should read: "as 13 S, Fig. 2B); whereas. . . "; the fifth line should read: "(Fig. 2A). The results. . .".
This content downloaded from 130.132.123.28 on Tue, 6 May 2014 06:58:16 AMAll use subject to JSTOR Terms and Conditions