control of lineage decisions in the embryonic kidney by ......sepideh sheybani deloui master of...
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Control of Lineage Decisions in the Embryonic Kidney by
Hedgehog Signalling
by
Sepideh Sheybani Deloui
A thesis submitted in conformity with the requirements
for the degree of Master of Science
Department of Physiology
University of Toronto
© Copyright by Sepideh Sheybani Deloui 2016
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Control of Lineage Decisions in the Embryonic Kidney by Hedgehog
Signalling
Sepideh Sheybani Deloui
Master of Science
Department of Physiology
University of Toronto
2016
Abstract
Foxd1-expressing cells give rise to stromal components in the mammalian embryonic kidney,
and are the major source of myofibroblasts during renal fibrosis. The signalling pathways
involved in segregation of the stromal and nephrogenic lineages from their common precursor
cells is not fully understood. Previous studies in the Rosenblum lab have shown the role of HH
signaling in expansion of cortical stromal gene expression and ureteropelvic junction
development. I present a stage-specific role for HH signalling in early renal precursors
expressing Sall1 and Osr1 in promoting stromal cell specification without affecting the
nephrogenic lineage. Temporal deletion of Ptc1 in Sall1+ and Osr1+ cells results in an early
increase in stromal cells, disorganization of the cortical stroma, medullary localization of cortical
stromal cells, decreased nephron number, and no change in cell proliferation. Mutant kidneys
exhibit UPJ obstruction at birth caused by ectopic cortical stromal cells in an activated state of
HH signalling.
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Acknowledgements
“The same wind that uproots trees
makes the grass shine.
The lordly wind loves the weakness
and the lowness of grasses.
Never brag of being strong.
The axe doesn't worry how thick the branches are.
It cuts them to pieces. But not the leaves.
It leaves the leaves alone.” ― Rumi, The Essential Rumi
My deepest gratitude goes first to Dr. Norman Rosenblum for giving me the opportunity to be
part of his research laboratory, for mentoring and guiding and me through this challenging
journey in the past two years. I would like to thank Norm for teaching me how to think outside
the box, to stay focused on my goals and to express myself confidently. Dr. Rosenblum is
certainly an exemplary role model in patience, compassion and genuinity.
An extension of my gratitude goes out to my supervisory committee members, Drs. Ian Rogers
and Martin Post. I have been immensely fortunate for having their continuing support, valuable
criticism and scientific advices. I would like to also thank Drs. Darius Bagli and Armando
Lorenzo for providing us with human patient samples from the Sickkids urology operation room.
I would like to thank each and every one of the members of the Rosenblum lab for their endless
kindness and support in the past two years. Meghan Feeney, for your patience showing me how
adorable the mice are, for always shining your smile and positivity to me. Tayyaba Jiwani for
sharing your expertise and supporting me through all the tough times I needed a hug to feel
better, for proofreading and critiquing my thesis. Priya Dhir, Chris Rowan, Samir Iskander and
Dana Kablawi for being such wonderful friends, together I made so many unforgettable
memories. Thank you for making me able to laugh when life was not so nice. Dr. Lin Chen for
being the source of courage and calmness in the lab. I would also like to thank Dr. Lijun Chi for
providing me with valuable advice and sharing her technical expertise.
I am very grateful to my family and friends for their continuing love and support. I would like to
specifically thank my parents Shirin and Mansour and my sister Sahar for encouraging me and
celebrating my achievements. Lastly, pursuit of this degree was facilitated by the NSERC
Alexander Graham Bell Canada Graduate Scholarship.
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Table of Contents
Acknowledgements .................................................................................................................................... iii Table of Contents ....................................................................................................................................... iv List of Tables ............................................................................................................................................. vii List of Figures ........................................................................................................................................... viii List of Abbreviations ................................................................................................................................. ix Publication of Thesis Work ....................................................................................................................... x Chapter 1: Literature Review ................................................................................................................. 1
1.1 Kidney Development ...................................................................................................... 2
1.1.1 Overview .......................................................................................................................... 2
1.1.2 Intermediate Mesoderm Specification and Patterning ........................................................ 4
1.1.3 Specification of the Metanephric Lineage ......................................................................... 7
1.1.4 Induction of Ureteric Bud Outgrowth and Branching Morphogenesis ............................... 10
1.1.5 Nephrogenesis ................................................................................................................. 12
1.2 Stroma............................................................................................................................ 14
1.2.1 Cellular Organization and Function of Renal Stroma ....................................................... 14
1.2.2 Developmental Origin of Renal Stroma ........................................................................... 18
1.2.3 Contribution of the Stroma to Renal Pathologies ............................................................. 20
1.3 Hedgehog Signalling ..................................................................................................... 24
1.3.1 Hedgehog Signalling Overview ...................................................................................... 24
1.3.2 Hedgehog Ligand in Vertebrates ..................................................................................... 27
1.3.3 Patched Receptor in Vertebrates ..................................................................................... 27
1.3.4 Advances in Defining the Role of Hedgehog Signalling in Kidney Development ............ 29
1.4 Ureteropelvic Junction (UPJ) Obstruction .................................................................. 30
1.4.1 Ureteropelvic Junction Obstruction in Humans ............................................................... 30
1.4.2 Underlying Causes of UPJ Obstruction ............................................................................ 31
1.5 Concluding Remarks, Hypothesis and Objectives ....................................................... 35
Chapter 2: Activated HH Signalling in Multipotent Renal Precursors Promotes Stromal Cell
Specification ....................................................................................................................... 37
2.1 Introduction .................................................................................................................. 38
2.2 Experimental Methods ................................................................................................. 40
2.2.1 Mouse Models ................................................................................................................ 40
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2.2.2 Whole Mount B-Galactosidase Staining ........................................................................... 41
2.2.3 Histology and Immunohistochemistry ............................................................................. 42
2.2.4 In situ mRNA Hybridization ........................................................................................... 43
2.2.5 RNA Isolation and Quantitative Real-Time Reverse Transcriptase- PCR ........................ 43
2.2.6 Flow Cytometry ............................................................................................................. 44
2.2.7 Automated Cell Quantification in Tissue Sections ........................................................... 45
2.2.8 Data Analysis ................................................................................................................. 45
2.3 Recombination in Sall1CreERt2 Mice is Tamoxifen Dependent and Specific to Sall1+
Cells............................................................................................................................... 46
2.4 Sall1+ Cells in the Caudal IM Contribute to Stromal and Nephrogenic Lineages ..... 48
2.5 Increased Hedgehog Signalling in Sall1+ Cells at E9.5 ................................................ 51
2.6 Increased Hedgehog Signalling in Osr1+ Cells at E9.5 ................................................ 59
2.7 Defect in Nephrogenesis in Ptc1 Mutant Kidneys ........................................................ 64
2.8 Discussion ..................................................................................................................... 68
2.8.1 Sall1 is expressed in the Precursors of Stromal and Nephron progenitors Between E9.5 and
E10.5. ............................................................................................................................. 68
2.8.2 Activation of HH Signalling in Precursors of Stromal and Nephron Progenitors Promotes
Stromal Cell Specification .............................................................................................. 69
2.8.3 Nephron Progenitor Specification and Maintenance are not affected by Induction of
Hedgehog Signalling Activity at E9.5 .............................................................................. 71
2.9 Conclusion .................................................................................................................... 73
2.10 Future Directions .......................................................................................................... 75
Chapter 3: Activated Hedgehog Signalling in Multipotent Renal Progenitors Leads to Prenatal
Hydronephrosis .................................................................................................................. 77
3.1 Introduction .................................................................................................................. 78
3.2 Experimental Methods ................................................................................................. 79
3.2.1 Intrapelvic Dye Injections ............................................................................................... 79
3.2.3 Patients’ Specimens Handling ........................................................................................ 79
3.2.4 Chromogenic Immunohistochemistry (IHC) ................................................................... 80
3.2.5 Quantification of IHC Staining ....................................................................................... 80
3.2.6 Acknowledgement of Contributing Colleagues ................................................................ 81
3.3 UPJ Obstruction and Hydronephrosis in Ptc1 Mutant Mice ....................................... 81
3.4 HH Signalling is Active in UPJ Obstruction in Mutant Mice ..................................... 86
3.5 Stromal Progenitors and Hedgehog Signalling Contribute To UPJ Obstruction in a
Subset of Human Neonates ........................................................................................... 88
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3.6 Discussion ..................................................................................................................... 93
3.7 Conclusion and Future Directions ............................................................................... 96
References ............................................................................................................................................. 98
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List of Tables
Table 1. List of primers for PCR analysis of mouse genomic day .............................................. 41
Table 2 Primer sequences used for qRT-PCR (Mus-musculus genes) ..................................... 44
Table 3. Primer sequences used for qRT-PCR (Homo Sapiens) ................................................ 80
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List of Figures
Figure 1 Overview of Mamallian Kidney Development Including Branching
Morphogenesis and Morphological Changes during Nephrogenesis .................................... 3
Figure 2 Specification of the Intermediate Mesoderm ...........................................................6
Figure 3 Development of the Rostral and Caudal Intermediate Mesoderm ..........................9
Figure 4 Structural Organization of the Prenatal Embryonic Kidney and Development of
the Renal Stroma .................................................................................................................... 16
Figure 5 Canonical Mammalian Hedgehog (HH) Signalling. ................................................ 26
Figure 6 Activated HH signalling in metanephic mesenchyme affects stromal gene
expression and leads to obstructive hydronephrosis ........................................................... 34
Figure 7 Recombination Specificity in Sall1CreERt2 Mice ......................................................... 47
Figure 8 Sall1+ cells transiently contribute to cortical stromal cells between E9.5 and E10.5
................................................................................................................................................. 50
Figure 9 Schematic of Ptc1 gene and confirmation of Ptc1 deletion and HH activity .......... 52
Figure 10 Ptc1 mutant kidneys display disorganized cortical stroma .................................. 54
Figure 11 Increased number of cortical and medullary stromal cells in Ptc1 mutant kidneys
................................................................................................................................................. 56
Figure 12 Proliferative capacity of Pbx1+ cells is not changed in Ptc1 mutants at E13.5 ....... 58
Figure 13 Foxd1 is increased in Ptc1 null Osr1+ cells .............................................................. 61
Figure 14 Stromal progenitor marker, Raldh2, is ectopically expressed in Osr1EGFPCreERt2;
Ptc1-null mutants .................................................................................................................... 63
Figure 15 Ptc1 mutant kidneys demonstrate a significant decrease in the number of
glomeruli at E18.5. ................................................................................................................... 65
Figure 16 Ptc1 mutant kidneys exhibit no marked defect in nephron progenitor
specification and maintenance .............................................................................................. 65
Figure 17 Nephron formation is modestly decreased in Ptc1 mutant kidneys ..................... 67
Figure 18 Activation of HH signalling in Sall1+ cells at E9.5 leads to abnormal pelvis and
UPJ development .................................................................................................................... 83
Figure 19 Activated HH signalling in Osr1+ cells at E9.5 leads to unilateral hydronephrosis
................................................................................................................................................. 85
Figure 20 Ectopic Ptc1 expression in the obstructed UPJ ............................................................. 87
Figure 21 Expression of stromal progenitors markers in the obstructed UPI compared to
the adjacent normal ureter in human neonates. ................................................................. 90
Figure 22 Expression of HH signalling response genes in the obstructed UPI compared to
the normal adjacent ureter in human neonates. .................................................................. 92
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List of Abbreviations
Anterior-Posterior ...................................................................................................................................... AP
Cap Mesenchyme ...................................................................................................................................... CM
Chronic Kidney Disease ......................................................................................................................... CKD
Congenital Obstructive Nephropathy...................................................................................................... CON
Cubitus Interruptus ...................................................................................................................................... Ci
Desert Hedgehog ...................................................................................................................................... Dhh
End Stage Renal Disease ...................................................................................................................... ESRD
Epithelial To Mesenchymal Transition ................................................................................................... EMT
Fluorescence Activated Cell Sorting ..................................................................................................... FACS
Glial Cell-Derived Neurotrophic Factor .............................................................................................. GDNF
Hedgehog .................................................................................................................................................. HH
Indian Hedgehog ...................................................................................................................................... IHH
Intermediate Mesoderm ............................................................................................................................. IM
Lateral Plate Mesoderm .......................................................................................................................... LPM
Lim Type Homeobox 1 ........................................................................................................................... Lhx1
Metanephric Mesenchyme ....................................................................................................................... MM
Nephric Duct ............................................................................................................................................. ND
Odd Skipped Related Gene ..................................................................................................................... Osr1
Paraxial Mesoderm ................................................................................................................................... PM
Patched ....................................................................................................................................................... Ptc
Quantitative Real-Time PCR .......................................................................................................... qRT-PCR
Red Fluorescence Protein ........................................................................................................................ RFP
Renal Vesicle ............................................................................................................................................ RV
Retinoic Acid ............................................................................................................................................ RA
Screted Frizzled-Related Protein ............................................................................................................. Sfrp
Smoothened............................................................................................................................................ SMO
Sonic Hedgehog ...................................................................................................................................... SHH
Tamoxifen .............................................................................................................................................. TAM
T-Box Transcription Factor 18 ............................................................................................................... Tbx1
Unilateral Ureteric Obstruction .............................................................................................................. UUO
Ureteric Bud .............................................................................................................................................. UB
Ureteropelvic Junction ............................................................................................................................. UPJ
Wolffian Duct .......................................................................................................................................... WD
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Publication of Thesis Work
Published Book Chapter:
1. Rowan CJ, Sheybani-Deloui S, and Rosenblum ND. Origin and Function of the Renal Stroma
in Health and Disease. In: Kidney Development and Disease, Miller RK (Ed), In Press.
Chapter one of this thesis borrows from published work #1.
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Chapter 1: Literature Review
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1.1 Kidney Development
1.1.1 Overview
The mammalian urogenital system, including the kidneys, is derived entirely from the
intermediate mesoderm (IM). The nephric duct (ND), also known as the mesonephric or
Wolffian duct (WD), arises from the rostral IM and extends caudally towards the hindlimb to
connect with the forming bladder (Little et al. 2012). ND-derived signals induce the formation of
primitive renal tubules from the adjacent nephrogenic mesenchyme in a distinct temporal
sequence along the anterior-posterior (AP) axis of the embryonic trunk, forming the pronephric
and mesonephric tubules (Figure 1) (Vainio et al. 1989). These primitive excretory structures
degenerate during mammalian embryonic life, with the remnants of the mesonephric tubules
contributing to the male gonads.
The developmental potential of the rostral IM becomes progressively restricted while a
caudal bean-shaped region of the nephrogenic mesenchyme commits to forming the metanephric
mesenchyme (MM) at the hindlimb level (Figure1) (Vainio et al. 1989). Beginning
approximately at day 11 of embryonic life (E11), the MM initiates formation of the permanent
functional kidney through a series of reciprocal signalling events with an ND- derived epithelial
structure called the ureteric bud (UB) (Wellik et al. 2002). These interactions lead to formation
of the collecting ducts, calyces, and pelvis, through the process of branching morphogenesis, as
well as formation of the glomeruli and the renal tubules, through the process of nephrogenesis
(Figure 1).
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Figure 1 Overview of Kidney Development Including Branching Morphogenesis and
Morphological Changes during Nephrogenesis
A. The pronephros (pro) and the nephric duct (ND) emerge from the cranial intermediate mesoderm. The
nephric duct extends and inserts in the forming bladder. Caudal to the pronephros is the mesonephros
(meso), which contributes to the male gonad. Metanephric mesenchyme (MM) is distinct by E10.5 and is
adjacent to the caudal end of the ND where the ureteric bud (UB) emerges. Signals from the MM induce
the UB to undergo a series of branching events to form the collecting system of the kidney B. The cells
surrounding the UB are induced to condense and form an aggregate known as the condensing
mesenchyme. A subset of cells within the condensing mesenchyme undergo a mesenchymal-to-epithelial
transition (MET), and a series of morphological changes to from the renal vesicle, comma-shaped, and S-
shaped bodies before maturing into nephrons.
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1.1.2 Intermediate Mesoderm Specification and Patterning
Due to the lack of molecular markers specific to the IM at the early post- gastrula stage,
defining the early IM remains an area of investigation. Odd-skipped related gene Osr1 encodes a
zinc-finger transcription factor expressed in the entire AP axis of the embryo and marks the
prospective lateral plate mesoderm (LPM) (Figure 2) (Ranghini et al. 2015; James et al. 2006).
Expression of Lhx1, a LIM-type homeobox gene, overlaps with that of Osr1 in the LPM and is
extended to the prospective IM (Dressler 2009; Tsang et al. 2000). Starting from E7.5-E8.75,
expression of Pax2/8 is detected exclusively in a band of cells just lateral to the paraxial
mesoderm (PM) (Bouchard et al. 2002). It was recently shown that in the absence of Pax2, IM
cells assume a paraxial mesodermal pattern of gene expression, suggesting that Pax2 may be
involved in defining the boundary between paraxial and intermediate mesoderm (Ranghini et al.
2015). Subsequent to Pax2 expression, Lhx1 becomes restricted to the IM and persists as the ND
starts forming and elongating caudally towards the hindlimb (Tsang et al. 2000). Around the
same time, Osr1 expression becomes restricted to the mesenchyme surrounding the IM and the
LPM- derivatives but is excluded from the ND itself (James et al. 2006).
Defects in formation, or absence of the ND in mice lacking either Lhx1, Osr1 or
Pax2/Pax8 highlight the critical importance of these genes in early specification and patterning
of the IM (Bouchard et al. 2002). Yet, relatively little is understood about the hierarchical
relationship and the regulatory organization among these genes. Comprehensive analyses of
knock-out mice revealed that Pax2/8 form the core of a gene regulatory network together with
the transcription factors Lhx1 and Gata3 , such that following activation by Pax2/8, the
regulatory molecules Gata3 and Lhx1 maintain each other's expression (Ranghini et al. 2015;
Boualia et al. 2013; Grote et al. 2006).
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Specification of the IM occurs not only along the AP axis, but also along the mediolateral
axis of the embryo. Activation of the aforementioned IM-specific genes, particularly Pax2/8, is
in part dependent on a balance between signals from the medial tissues, neural tube and somites
in addition to signals from the more dorsolateral ectoderm (Reviewed in (Dressler 2009;
Takasato et al. 2015). Although low concentrations of BMPs, and a proper balance of both
Activin and Retinoic acid (RA) signalling are shown to induce IM at the anterior pole, it is still
not clear whether the same set of signals also control IM induction at the posterior end (Kim et
al. 2005; Obara-Ishihara et al. 1999). It has been proposed that a specific pattern of Hox gene
expression, distinct from the established posterior Hox combination, primes the mesoderm to
respond to IM inductive signals at the anterior boundary, which initiates expression of Lhx1,
Pax2 and Pax8 (Preger-Ben Noon et al. 2009). By contrast, the Hox11 group of genes is needed
to distinguish the posterior mesoderm from the more anterior mesonephric tissue (Wellik et al.
2002). Recent evidence suggests that differential Hox gene expression may contribute to early
lineage decisions and AP patterning of the mesoderm through influencing epigenetic
mechanisms, and thus the genomic accessibility of anterior and posterior targets of the IM-
inducing signals (Soshnikova et al. 2009).
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Figure 2 Specification of the Intermediate Mesoderm
Schematic of a flattened E8.5 mouse embryo (dorsal view). A. Osr1 expression (blue) encompasses the
lateral plate mesoderm and the presumptive IM, and extends more anteriorly and more laterally than Lhx1
(yellow) in (B). B. Lhx1 (yellow) is expressed just lateral to the paraxial mesoderm (pink). Pax2 and Pax8
expression (brown) has an anterior border at approximately the sixth somite, specifically marking the IM
as it extends caudally. Following Pax2 expression, Lhx1 expression becomes restricted to the IM.
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1.1.3 Specification of the Metanephric Lineage
As previously discussed, Osr1 is expressed in the IM before any of the other MM-
regulatory genes. Osr1 expression is excluded from the ureteric epithelial lineage at E9.5, while
it remains strongly expressed in the differentiating MM from E9.5-E15.5 (James et al. 2006).
Moreover, generation of Osr1 knockout mice demonstrated the indispensable role of Osr1 in the
initial formation of the MM. Expression of several key regulators of MM development including
Six1/2, Pax2 and Sall1 are disrupted in the absence of Osr1, leading the newly formed MM to
undergo apoptosis (James et al. 2006). In addition to Osr1, there are a number of genes
expressed in the undifferentiated MM including Pax2, Six1, and Sall1, but they are all
dispensable for the initial formation of the MM, and are instead required for its subsequent
differentiation and/or survival (Gong et al. 2007; Brodbeck et al. 2004; Nishinakamura et al.
2001).
Analysis of transcription factor expression distinguishes two morphologically distinct
populations in the induced MM (E11.5): a Six2+ nephrogenic mesenchyme, and an outer layer of
Foxd1+ cortical stromal cells that emerge from the Osr1+ caudal IM (Figure 3B). These
populations form two mutually exclusive progenitor compartments shortly after the onset of
ureteric branching, as there is no compelling evidence suggesting contribution of Foxd1+ cell to
the Six2+ pool or vice versa during mammalian kidney development (Mugford et al. 2009;
Kobayashi et al. 2014). A lineage tracing study by Mugford et al.(2008) demonstrated the
contribution of Osr1+ cells pulse labelled between E8.5- E10.5 to both Six2+ nephron
progenitors and Foxd1+ stromal progenitors, while Osr1+ cells labelled at E11.5 onwards were
restricted to the nephrogenic lineage (Mugford et al. 2008). Moreover, it was uncovered that
Osr1+ cells give rise to Flk1+ endothelial progenitors, and are therefore known as the
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multipotent progenitors of the kidney (Mugford et al. 2008). However, utilizing mice lacking
OSR1 activity, OSR1 function was found to be crucial for the establishment of the nephron
progenitor pool, but not for specification of the stromal lineage. These data suggest that although
Osr1+ cells give rise to multiple lineages in the kidney, Osr1 expression itself does not regulate
lineage decisions within the IM (Mugford et al. 2008)
Investigating the origin of Osr1+ cells is key to understanding the origin of the MM.
Until recently, all the different lineages involved in kidney development, including the
nephrogenic and stromal progenitors, as well as the ureteric epithelium, were thought to originate
from the elongated rostral IM (Dressler 2006). However, investigation of Gata3, a transcription
factor expressed in the ND (between E8.5 to E10) and the ND-derived UB, challenged this
notion (Figure 4B) (Grote et al. 2006). In the absence of Gata3, the rostral IM fails to elongate
caudally and the UB fails to form, while the Pax2-expressing MM persists (Grote et al. 2006).
These data suggest that the MM develops independently of the Gata3+ ND-derived UB lineage.
Inducible labeling of caudal IM cells expressing Eya1, a transcription factor essential for the
initial formation of the MM, demonstrated the nephron-restricted fate of these cells, and no
contribution to ND-derived structures (Figure2B) (Gong et al. 2007; Jinshu Xu et al. 2014).
Brachyury (T) is a marker of immature caudal presomitic mesoderm (PSM) that persists in the
posterior end of the embryo (Figure 4A). Strikingly, investigation of the fate of T+ cells labelled
at E8.5 demonstrated the contribution of these cells to the MM. Labelled cells were not detected
in the ND, providing further evidence that the MM arises from late caudal IM marked by T
expression (Taguchi et al. 2014). Based on these data, it is now believed that caudal trunk
progenitors that lose T expression and start expressing Osr1 are the multipotent precursors of the
stromal and nephrogenic lineages within the MM field (Takasato et al. 2015).
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Figure 3 Development of the Rostral and Caudal Intermediate Mesoderm
A. A diagram illustrating the elongating anterior IM (orange) and the nascent T+ presomitic or posterior
mesoderm (green) in an E8.0 embryo. B. Cross section of an E8.5 embryo at the position marked with a
dashed line in A, illustrating the Gata3+ anterior IM (orange) and the newly formed Eya1+ posterior IM
(green) derived from the T+ posterior mesoderm. The fate map of the anterior and posterior IM is shown
from E8.5 to E11.5. The ND is derived from the elongating anterior IM by E9.5. The ND gives rise to the
UB which invades the MM and starts branching at E11.5. Osr1+ precursors of the nephrogenic and
stromal lineages are located in the forming MM derived from the posterior IM (green). Tbx18+ cells
(pink) are located adjacent to the ND and the MM, and contribute to both stromal progenitors and the
ureteric mesenchyme. At E11.5 Six2+ nephrogenic (green) and Foxd1+ stromal progenitors (red) (NP and
SP, respectively) are specified within the Osr1+ progenitor pool.
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1.1.4 Induction of Ureteric Bud Outgrowth and Branching Morphogenesis
Glial cell-derived neurotrophic factor (GDNF) is secreted by the MM adjacent to the
dorsal ND, and triggers pseudostratification and invagination of the UB (Sainio et al. 1997).
Gdnf expression is shown to be under positive regulation of several transcription factors (Eya1,
Pax2 and Six1/4) expressed within the MM (Brodbeck et al. 2004). GDNF signalling occurs
through the RET receptor tyrosine kinase (RTK) and its co-receptor GFRα1, both of which are
expressed on the ureteric epithelia (Gong et al. 2007; Chi et al. 2009). Ret expression is
necessary for initial UB formation as well as the subsequent branching morphogenesis, likely
because GDNF-driven proliferation at the UB tips is required for the formation and growth of the
ureteric branches (Costantini et al. 2006).
Since the receptors required for GDNF signalling are expressed on the entire length of the
ND, spatial restriction of Gdnf signalling to the posterior domain of the IM is critical for
specification of the UB site and proper UB outgrowth (Sainioet al., 1997). Foxc1 and Foxc2
encode two transcription factors in the uninduced MM which function to restrict cranial
expansion of Eya1 and Gdnf signalling domain (Kume et al. 2000). Moreover, SLIT2 is a large
secreted protein, strongly expressed in the anterior ND which mediates ROBO2 activation in the
nearby nephrogenic mesenchyme (Grieshammer et al. 2004). This intracellular signalling system
has been demonstrated to restrict Gdnf signalling to the posterior end through a yet unexplained
mechanism (Grieshammer et al. 2004). In addition to the fairly well characterized feed forward
mechanism of the interplay between RET and GDNF in initiating UB formation, BMP4 is
expressed in the tail bud mesenchyme surrounding the ND and MM, and has been shown to
promote UB outgrowth at the correct budding site relative to the ND (Miyazaki et al. 2000).
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Once the nascent UB invades the MM, it undergoes a process of iterative branching that
generates the urinary collecting system through reciprocal signalling interactions between the
ureteric epithelial, nephrogenic and stromal mesenchymal lineages within the kidney. The
developing ureteric tree can be segmented into ureteric tips and stalks (Figure 1A). Gdnf is
expressed in the cap mesenchyme (CM) surrounding the ureteric tips and is a potent inducer of
the Ret signaling pathway, which is essential for branching (Costantini 2010). Wnt11 is
expressed in the ureteric tips and maintains Gdnf expression in the metanephric mesenchyme to
mediate a feed-forward signaling mechanism, through a yet unknown mechanism (Majumdar et
al. 2003; Cain et al. 2009). Ureteric branching requires that UB tips remain responsive to GDNF.
β-Catenin (Ctnnb1) is expressed in the surrounding renal stroma and has been shown to mediate
canonical Wnt signalling to maintain Ret expression in the UB tips and thereby, elicit GDNF
responsiveness (Bridgewater et al., 2008). Furthermore, RA (vitamin A) signalling, via RA
receptors RARα and RARβ2, is crucial for regulation of branching morphogenesis through up-
regulation of Ret expression in the UB tips (Mendelsohn et al. 1999; Batourina et al. 2001). RA
is synthesized from retinaldehyde in the cortical stroma and the UB by the enzymes RALDH2
and RALDH3, implying an additional non-cell autonomous role of stroma in promoting
branching (Li et al. 2000; Mendelsohn et al. 1994; Rosselot et al. 2010). Several other signalling
pathways including FGF as well as canonical and non-canonical BMP signalling are also
required to regulate the branching process (reviewed in (Blake et al. 2014)). Yet, the integration
and interplay of these various pathways is not fully understood.
In addition to branching morphogenesis, elongation of the epithelial ureteric trunk to the
medullary region is essential for formation of a functional collecting duct where urine is
concentrated and excreted into the ureter. The cortico-medullary axis of kidney organization and
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function is regulated by Wnt7b signaling, whose expression is restricted to the elongating
component of the collecting duct system (Yu et al. 2009). Removal of β-catenin from the
underlying Wnt-responsive interstitium phenocopies the medullary deficiency of Wnt7b mutants,
suggesting a paracrine role for Wnt7b action through the canonical Wnt pathway (Park et al.
2007). In addition to the canonical Wnt signalling pathway, WNT9b and WNT7b act through the
non-canonical pathway to mediate the planar-cell-polarity (PCP) process responsible for
reorganization and interdigitation of the ureteric epithelial cells, allowing for UB elongation
(Karner et al. 2009; McNeill 2009).
1.1.5 Nephrogenesis
The elongating UB extends toward the source of GDNF, and meets the MM cells at around
E10.5-E11.0 (Kopan et al. 2014). This induces MM cells to organize a cohesive domain of cells
around the UB tips, called the Cap Mesenchyme (CM) (Figure 4). CM contains self-renewing
nephron progenitors, characterized by expression of key transcriptional regulators including Six2,
Cited1, Osr1 and Sall1. Lineage tracing studies have established that Six2+ nephron progenitors
contribute to podocytes as well as all the other epithelial cells of the mature nephron (Figure 4).
Ex vivo kidney cultures treated with FGF-2 and FGF2- like proteins have demonstrated the
importance of FGF signalling through the intracellular mediator RAS in establishment of the CM
cells from their precursors in the posterior IM as well as in the self-renewal of nephron progenitors
(Brown et al. 2011).
It is well established that a delicate balance of self-renewal and differentiation is required
throughout embryogenesis to ensure the formation of the full complement of nephrons.
Premature differentiation of CM cells upon deletion of Six2, or signalling factors such as Fgf9 or
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Fgf20 leads to depletion of the progenitor pool, and kidney formation is halted (Kobayashi et al.
2008; Self et al. 2006). It has been proposed that relatively high levels of nuclear β-catenin
together with reduced Six2 expression in Cited1-negative subset of nephron progenitor cells
favors MET over self-renewal (Brown et al. 2013; Park et al. 2012). By contrast, high levels of
Six2 in a transcriptional complex with members of the lymphoid enhancer factor/T cell factor
(TCF/LEF) family of transcription factors, prevent differentiation induced by β-catenin (Park et
al. 2012). Recent studies have uncovered the role of Sall1 upstream of Six2 to maintain an
appropriate level of Six2 expression in the CM, so that only a subset of cells are committed to
differentiation at each UB tip (Basta et al. 2014; Kanda et al. 2014). SALL1 also acts as a
transcriptional repressor recruiting the Mi2/NuRD chromatin remodeling complex in
differentiating nephrons to repress β-catenin transcriptional output, thus inhibiting premature
differentiation (Ohmori et al. 2015; Denner et al. 2013). Additionally, it was recently
demonstrated that OSR1 and SIX2 act synergistically to form a strong repressor complex with
TCF/LEF to prevent the activation of WNT/β-CATENIN target genes, such as Wnt4 in the CM
(Jingyue Xu et al. 2014).
Once a subset of CM cells at a new branch tip undergo MET in response to WNT9b-
dependent signals from the UB renal vesicle (RV) forms at the “arm-pit” of the ureter (Carroll et
al. 2005). Here, WNT9b acts through the canonical Wnt signalling pathway and is upstream of all
the known markers of nephrogenesis, including Wnt4, and Lhx1 (Karner et al. 2011).
Undifferentiated, GDNF-secreting CM surrounds the leading edge of the UB tip towards the
kidney periphery, thereby stimulating continued branching (Figure 1B) (Park et al. 2007). In
addition, Sall1 expression within the undifferentiated MM restricts Wnt9b expression to the
ureteric stalk, to allow for nephron progenitor differentiation without exhausting the progenitor
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pool and halting UB branching (Basta et al. 2014; Kanda et al. 2014). Subsequent differentiation
of distinct segments of the intermediate structures involves cell proliferation, extensive tubular
elongation, and proximal-distal patterning (Kobayashi et al. 2005; Karner et al. 2009; Little et al.
2012; Kanda et al. 2014). Once an RV is formed, its proximal portion elongates and fuses with
the UB tip closest to its point of initiation to form the comma-shaped body (Figure 1). The distal
portion of the comma-shaped body further elongates and forms the S-shaped body (Figure 1)
(Georgas et al. 2009). The molecular mechanisms involved in segmentation and maturation of the
S-shaped body into proximal, glomerular, and distal portions are not completely understood. It is
known that Notch2 signaling in nephron progenitors is required for establishing proximal-distal
patterning of the developing nephron, but is less important in differentiation of the glomerulus
itself (Cheng et al. 2007).
1.2 Stroma
1.2.1 Cellular Organization and Function of Renal Stroma
In addition to the nephrogenic mesenchyme and ureteric epithelium, a third major cell type
is present during early kidney organogenesis – the stromal cell population (Figure 3). A member
of Forkhead transcription factors, Foxd1, is the first gene that distinguishes stromal cells from the
nephrogenic mesenchyme as early as E10.5 (Figure 3). It was shown that Foxd1+ cells are first
observed anterior to the MM and as development progresses, Hox10 mediates posteriorization and
integration of these cells into the periphery of nephrogenic zone (Yallowitz et al. 2011). Foxd1+
cells are also observed interior to the nephrogenic zone, occupying spaces between ureteric bud
branches and induced nephrons (Figure 4A). Until recently, the origin and interrelationship
between these stromal components were poorly understood. However, fate-map analysis of
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Foxd1+ cells established that this population contains multipotent self-renewing progenitors
giving rise to renal capsule, cortical and medullary interstitial cells, mesangial cells, and pericytes
of the kidney (Kobayashi et al. 2014; Hum et al. 2014). It is still not clear whether Foxd1+
population in the capsule and nephrogenic zone is a homogenous population or contains different
domains with distinct differentiation capacities, similar to the subdomains in the CM (Mugford
et al. 2009; Kobayashi et al. 2014).
The embryonic kidney is characterized by an inner medullary and outer cortical zone, both
of which contain stromal elements derived from Foxd1+ stromal progenitors (Figure 4A). Stromal
cells of the renal capsule envelop the kidney as a continuous layer of flattened cells, and strongly
express Sfrp1, a secreted frizzled-related protein that antagonizes Wnt4 signalling, preventing the
self-renewing nephron progenitors from epithelialization (Levinson et al. 2005; Yoshino et al.
2001). Directly beneath the renal capsule lie round-shaped cortical stromal cells that intercalate
and surround the induced nephrogenic structures (Figure 4A). In addition, vascular smooth muscle
cells, pericytes, and mesangial cells are essential components of the glomerular and peritubular
vessels surrounding endothelial cells throughout the kidney (Figure 4B). Pericytes and mesangial
cells are involved in the glomerular vasculature response to various physical stimuli, and in the
regulation of renal hemodynamics. Lastly, resident fibroblasts are located in both the outer
nephrogenic zone and inner medullary region interspersed between tubules and nephrons, and
contain supportive fibroblast cells and ECM components.
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Figure 4 Structural Organization of the Prenatal Embryonic Kidney and Development of the Renal Stroma
Stromal cells are present in various locations in the prenatal kidney (E18.5). A. A schematic showing the
outer nephrogenic zone and inner medullary zone containing stromal elements including cortical stroma
(pink), renal capsule (dark purple) and medullary stroma (light purple). A glomerulus is outlined in the
dotted black box. B. The glomerulus contains stromal derivatives including pericytes (pink), mesangial
cells (brown) and endothelial cells of the renal vasculature (black). Abbreviations: NP = nephron
progenitors, NZ = nephrogenic zone.
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Stromal progenitors play essential roles in nephron progenitor differentiation through non-
cell autonomous signalling pathways (Reviewed in (Li et al. 2014)). Homozygous deficiency of
Foxd1 causes kidney capsule defects characterized by a thickened, histologically abnormal renal
capsule, decreased expression of the stromal/capsule markers Raldh2 and Sfrp1, expansion of the
nephron progenitor population, and perturbed nephrogenesis (Levinson et al. 2005; Hatini et al.
1996). It has been suggested that failure to form a discrete nephrogenic zone and juxtamedullary
region (as illustrated in Figure 4A) in Foxd1 mutants is causative in delaying nephron
differentiation (Levinson et al. 2005). In addition, a more recent study demonstrated the non-cell
autonomous role of FOXD1 in BMP7-mediated transition of nephron progenitors to WNT-induced
differentiating state by supressing Decorin, a small antagonizer of BMP/SMAD signaling (Fetting
et al. 2014). Furthermore, loss of capsular expression of Sfrp1, a secreted WNT inhibitor, leads to
a slight increase in the number of glomeruli, suggesting that stromal Sfrp1 may also act to restrict
WNT4-induced nephron progenitor differentiation to generate the appropriate number of nephrons
(Trevant et al. 2008).
Reduced UB branching and abnormal branch patterning in Foxd1-deficient mice suggested
a non-cell autonomous role of stromal signalling in regulating renal branching morphogenesis
(Hum et al. 2014). Similarly, mice lacking Pbx1, a transcription factor expressed highly in the
stroma, share phenotypic characteristics with Foxd1-deficient mice, demonstrating abnormal
branching due to the expansion of Ret expression from the ureteric tip to the stalk (Schnabel et al.
2003). Since Foxd1 expression is not affected in Pbx1-deficient mice, it is likely that Pbx1 plays
a role in renal branching morphogenesis and ureteric tip specification through mechanisms similar
to but independent from Foxd1 (Schnabel et al. 2003). Cortical stromal RA, synthesized by the
enzyme RALDH2, is also an important mediator of ureteric branching through Rarβ2-dependent
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regulation of Ret expression in the UB. Rarβ2-deficient mice demonstrated a reduction in the
number of branches in addition to a mis-patterned capsular layer similar to Foxd1 and Pbx1
deficient mice (Mendelsohn et al. 1999; Batourina et al. 2001). Although, as discussed above,
many models of stromal disruption have been shown to display overlapping phenotypes (i.e.
expanded nephron progenitor domain), little has been done to investigate coordination or
integration of various signalling pathways (Bagherie-Lachidan, Reginensi, Pan, et al. 2015;
Ohmori et al. 2015).
1.2.2 Developmental Origin of Renal Stroma
The molecular mechanisms underlying differentiation of the distinct stromal and
nephrogenic lineages from the common progenitor lineage remain poorly defined. However,
studies defining T-box transcription factor 18 (Tbx18) expression in the urogenital region have
provided some insight into the spatiotemporal pattern of stromal lineage specification. Tbx18
was previously known as a marker of the undifferentiated ureteric mesenchyme. Careful analysis
of Tbx18 expression at several developmental stages demonstrated that it is also present in a
narrow band of cells between the mesenchyme surrounding the ND and the metanephros at
E11.5 (Figure 3B) (Airik et al. 2006; Bohnenpoll et al. 2013a). Lineage tracing of Tbx18+ cells
in addition to the spatial localization of Tbx18+ cells demonstrates a contribution of a spatially
restricted population of Tbx18+ cells to the descendants of the Foxd1+ lineage (Bohnenpoll et al.
2013b). Hence, it was postulated that at E10.5, in addition to the already specified Foxd1+ and
Six2+ committed lineages, there are uncommitted Tbx18+ cells within the Osr1+ MM that can
give rise to either stromal or ureteric mesenchymal lineages. According to Bohnenpoll et al
(2013), it is likely that Tbx18+ cells that do not differentiate into the mesenchymal cell types of
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the ureter lose Tbx18 expression between E10.5-E11.5 either die or contribute to the renal stroma
through an as yet undefined mechanism (Bohnenpoll et al. 2013b). However, the mechanism by
which lineage decisions are made within the common Osr1+ IM to separate nephrogenic and
ureteric mesenchyme/stromal progenitors remains to be determined.
Studies in the chick and mouse embryo suggest the existence of a distinct progenitor of
renal stromal cells residing outside the IM. Retroviral gene transfer techniques followed by fate-
mapping analysis in the chick embryo identified the adjacent PM as the principle source of the
renal stroma. This was evident by localization of β-gal+ cells to the Foxd1+ stromal zone of the
kidney only when LacZ-encoding retrovirus was injected to the PM (Guillaume et al. 2009).
Moreover, a recent high-throughput gene expression analysis in mice lacking Pax2 provided
indirect evidence that lower Pax2 expression in the mammalian PM might govern the
contribution of the PM to the renal stroma (Ranghini et al. 2015). When Pax2 is deficient in the
IM, IM cells are characterized by a pattern of gene expression that is more consistent with the
PM, and genes normally expressed in common derivatives of Foxd1+ progenitors are
upregulated (Ranghini et al. 2015). Therefore, it was hypothesized that after Osr1 expression is
restricted to nephron progenitors at E11.5, new stromal progenitors likely arise from the PM, and
migrate to the induced MM. Yet, to date, there has been no direct in vivo evidence demonstrating
the contribution of PM cells to the renal stroma.
In summary, the published body of work strongly suggests that a Osr1+ progenitor pool
derived from the T+ PSM has the potential to give rise to both stromal and nephron progenitors.
Further studies have shown the presence of a subpopulation of Osr1+ cells expressing Tbx18 that
contributes to both ureteric mesenchyme and the renal stromal lineage. It is also likely that
stromal progenitors are a heterogeneous population of cells, a subset of which are derived from
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the PM. Nevertheless, the molecular mechanisms and signalling pathways regulating the
separation of the stromal lineage from either nephrogenic lineage or ureteric mesenchyme has
remained poorly understood.
1.2.3 Contribution of the Stroma to Renal Pathologies
1.2.3.1 Horseshoe Kidney
During normal renal development kidneys flank the midline of the embryo before
separating from the body wall, rotating and then separating from each other (Fotter
2008).Kidneys maintain their connection to the dorsal body wall through a thin layer of
connective tissue until approximately E14.5 (Levinson et al. 2005; Kobayashi et al. 2014). In
Foxd1-null mutants where the capsule does not form properly, kidneys remain attached to the
body wall and do not ascend to the lumbar region (Hum et al. 2014; Hatini et al. 1996). Given
the strong Foxd1 expression in the connective tissue surrounding the kidney and the renal
capsule, it is plausible that FOXD1-mediated capsule formation results in kidneys detaching
from the dorsal body wall (Levinson et al. 2005). A similar phenomenon occurs in human
patients where failure of proper kidney ascension results in ‘horseshoe kidneys’ with an
incidence rate of one in 400-600 live births. Horseshoe kidneys are fused at the lower pole,
forming a U or horseshoe shape. Failure of ascension also results in the ‘pelvic kidney’ in which
the kidney fails to ascend from the pelvis (Nemes et al. 2015; Weizer et al. 2003). In some cases
the horseshoe kidneys can present with ureteropelvic obstruction leading to urinary tract
infections, abdominal mass, and hematuria (Rodriguez 2014).
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1.2.3.2 Renal Fibrosis
Chronic kidney diseases (CKD) regardless of their primary cause, lead to the loss of renal
function, and are histologically characterized by fibrosis where functional renal tissue is replaced
by permanent interstitial fibrotic tissue (Boor et al. 2012). Fibrotic scar tissue disrupts normal
organ structure and hinders regeneration and normal function. The key characteristics of renal
fibrosis are the extensive deposition of ECM and expansion of a distinct α-SMA+ population of
fibroblasts called myofibroblasts (Grgic et al. 2014; Falke et al. 2015). By definition,
myofibroblasts are contractile cells that are the principle source of interstitial collagens,
including fibrillar collagens I and III (Grgic et al. 2014).
Studying the exact origin of myofibroblasts during CKD can have implications for anti-
fibrotic therapies, but is also challenging due to the lack of markers specific to myofibroblasts. In
vitro studies initially suggested that myofibroblasts form via transition of epithelial cells to a
mesenchymal phenotype, a process termed epithelial to mesenchymal transition (EMT) (Cheng
et al. 2010; Iwano et al. 2002). Expression of αSMA has been detected in tubular and glomerular
epithelia in association with disease progression, in both a remnant kidney model and during
experimental glomerulonephritis (Iwano et al. 2002). However, lineage tracing studies using an
epithelial cell specific γ-glutamyl transpeptidase (GT)-driven GFP and Cadherin(Cdh)16
promoter in a model of obstructive nephropathy failed to detect the contribution of epithelial
cells to the myofibroblast pool, raising doubt as to the epithelial origin of the myofibroblasts (Li
et al. 2010; Iwano et al. 2002). More recently, studies by Humphreys et al (2010) reported a
marked expansion of pulse labelled interstitial cells that expressed α-SMA, following either
complete unilateral ureteric obstruction (UUO) or ischemia-reperfusion injury (Grgic et al. 2012;
Humphreys et al. 2010). In fact, an elegant lineage-tracing analysis by Humphreys et al (2010)
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revealed that majority of myofibroblasts originate specifically from Foxd1+ derived percicytes
(Humphreys et al. 2010). Interestingly, lineage tracing of both nephrogenic and UB-derived
elements using Six2Cre and Hoxb7Cre demonstrated no contribution to myofibroblasts in both
UUO and IR model of fibrosis, contradicting the initially thought contribution of EMT process to
the myofibroblast pool (Humphreys et al. 2010).
Although Foxd1+ stromal progenitors are potentially important contributors to the
fibrotic response, the factors regulating the differentiation of pericytes and interstitial fibroblasts
to myofibroblasts in response to injury are not clear. Recent studies suggest that activated
PDGFR signalling in response to injury leads to proliferation and differentiation of the pericytes
to myofibroblasts, and that TGFβ1 may be an important contributing cytokine in transducing
activated PDGFR signalling (Chen et al. 2011). Moreover, it is shown for the first time in an in
vivo model of fibrosis that anti-PDGFR antibody administration markedly decreases the number
of a-SMA+ myofibroblasts (Chen et al. 2011). Contrary to the aforementioned findings, other
studies using more mature pericyte markers, PDGFRβ- and NG2- promoter–driven mice to
functionally deplete pericytes in models of kidney injury did not result in any significant
reduction in fibrosis (LeBleu et al. 2013). Similarly, lineage tracing of NG2+ and PDGFRβ+
pericytes did not show a predominant contribution of labeled cells to the myofibroblast
population in the fibrotic kidney (LeBleu et al. 2013).
New evidence has implicated Hedgehog (HH) signalling as a major contributor to the
fibrotic response. It was shown that pericytes express HH effectors Gli1 and Gli2, and that Gli1+
pericytes proliferation is great enhanced (11-fold greater) during fibrosis (Fabian et al. 2012).
Further evidence to support HH signalling as a key contributor to fibrosis was shown using
lineage tracing of Gli1+ cells in the kidney after acute injury (Kramann, Schneider, et al. 2015).
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Genetic ablation of tissue-resident Gli1+ mesenchymal stem cells protected against injury-
induced renal fibrosis (Kramann, Schneider, et al. 2015). It was further shown that GLI2
inhibition prevented myofibroblast progression and limited the fibrotic response (Kramann,
Fleig, et al. 2015). These data suggest a key regulatory role for GLI2 in inducing myofibroblast
formation from pericytes in response to injury. Further studies are needed to fully delineate the
mechanism and interactions of HH signalling in the fibrotic response.
Overall, there are discrepancies concerning the origin of myofibroblasts in CKD most
likely due to the limitations in the available studies, including the use of overlapping markers,
different mouse strains, choice of injury model and the duration and severity of the injury
affecting the outcome (Falke et al. 2015). A thorough and detailed analysis of the lineage and
signalling mechanisms of the stroma may allow for a greater understanding of the functional
significance of this population. These new insights could provide a foundation for the
development of more effective treatment options and outcomes of fibrosis in patients.
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1.3 Hedgehog Signalling
1.3.1 Hedgehog Signalling Overview
Hedgehog family of proteins are a family of secreted molecules that play an evolutionarily
conserved role in patterning and development of many metazoans. Hedgehogs were first identified
in the fruit fly Drosophila melanogaster (Chen et al. 1996). It is now established through an
extensive array of studies that HH is also important in vertebrates for fundamental processes such
as patterning the left-right body axis, AP skeletal patterning, angiogenesis, hematopoiesis,
chondrogenesis, and development of the cerebellum, craniofacial structures, limbs, lung, kidney
and gastrointestinal tract (Reviewed in (Ingham et al. 2001; Briscoe 2013)).
Drosophila HH ligand binds to its receptor, Patched (Ptc), on the plasma membrane,
relieving PTC-mediated inhibition of Smoothened (Smo), a transmembrane protein expressed on
the cell surface (Hooper et al. 1989; Rohatgi et al. 2007). In the presence of HH, SMO interacts
with a molecular complex consisting of Costal-2 (Cos2), Fused (Fu), and Suppressor of Fused
(SuFu). Interaction of SMO with COS2 confers FU activity and SUFU inactivity (Méthot et al.
2000). In this state, full-length Cubitus interruptus (Ci), the Drosophila GLI ortholog, translocates
to the nucleus where it acts as a transcriptional activator (Jiang 2006). In the absence of HH, PTC
inhibits the interaction between SMO and COS2, allowing COS2 to bind and retains Ci in the
cytoplasm. The presence of active SUFU in the COS2-FU-SUFU-Ci complex permits cleavage of
Ci to its N-terminal form, which localizes to the nucleus and acts as a transcriptional repressor
(Galimberti et al. 2012).
In vertebrates there are three different homologs of Ci protein – GLI1, GLI2 and GLI3 –
which are members of the glioblastoma family of transcription factors and contain zinc finger
DNA binding domains to activate and repress transcription of target genes (Figure 5) (Mo et al.
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1997). Vertebrate GLI proteins are regulated through similar cleavage and truncation processes
as Ci protein in Drosophila. Moreover, a dynamic interplay between GLI activators and
repressors is critical during mammalian organogenesis (Buttitta et al. 2003; Hu et al. 2006).
GLI1 and GLI2 exist predominantly as full length proteins in cultured mammalian cells and act
as transcriptional activators during murine embryogenesis (Bai et al. 2002). In contrast, GLI3
exists primarily in its N-terminal truncated form which acts as transcription repressor; thus, the
predominant activity of GLI3 is to repress expression of HH dependent genes (Bai et al. 2002;
Park et al. 2000). Analysis of mice genetically engineered to express the truncated form of GLI3
has demonstrated the pathogenic role of constitutive GLI3 repressor activity during murine
embryogenesis (Grotewold et al. 2002). In support of a deleterious role of GLI3R in
embryogenesis, studies have reported that genetic elimination of Gli3 in the Shh-null background
rescues HH mutant phenotypes in embryonic tissues including neural tube, limb, face, forebrain
skin, and kidney (Litingtung et al. 2000; Litingtung et al. 2002; Rallu et al. 2002; Mill et al.
2005; Hu et al. 2006).
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Figure 5 Canonical Mammalian Hedgehog (HH) Signalling.
In the presence of HH ligand, SMO translocates to the primary cilium. Activation of SMO inhibits the
proteolytic processing of GLI proteins and allows full length GLIfl proteins to enter the nucleus to activate
transcription of HH target genes. GLI1 and GLI2 have predominant activator roles while GLI3 acts as the
main transcriptional repressor of HH signalling. In the absence of HH ligand, the receptor PTC represses
the activity of the transmembrane protein SMO and its localization to the primary cilium. Full length GLI
proteins (GLI FL) associate with the proteolytic complex, which promotes the proteasome-dependent
processing or complete degradation of the GLI transcription factors. Truncated forms of GLI proteins
(GLIR) translocate to the nucleus and act as transcription inhibitors.
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1.3.2 Hedgehog Ligand in Vertebrates
Unlike the single HH gene found in Drosophila (Hh), three different HH genes have been
found in vertebrates. These include Desert hedgehog (Dhh), Indian hedgehog (Ihh), and Sonic
hedgehog (Shh) (Astorga et al. 2007). Diverse expression patterns of these three HH genes results
in their specialized functions via signalling pathway highly similar to that in Drosophila signalling
pathway. During early embryogenesis, the role of Dhh is largely restricted to germ cell
development, as it is expressed in Sertoli cells in the testes and granulosa cells in the ovaries (Yao
et al. 2002). Shh is the most broadly expressed homolog and plays a well- defined role in patterning
of the neural tube, as well as in limb bud, olfactory pathway, eye, heart, lung and gut development
(Ingham et al. 2001; Jr 2010). Ihh, the most closely related homolog to Shh, is expressed in the
primitive endoderm and plays a role in bone and skeletal development.
Of the three mammalian HH members, only Shh and Ihh expression has been identified
in the embryonic kidney (Fabian et al. 2012). SHH is found in the urothelium and the
presumptive ureter as early as E11.5, and in the distal collecting duct at E14.5 (Yu et al. 2002;
Hu et al. 2004). In newborn kidneys, Shh continues to be expressed in the collecting duct in
addition to the inner medulla, and renal pelvis (Fabian et al. 2012; Hu et al. 2004). On the other
hand, Ihh expression is reported to be restricted to the nephron epithelia in the cortex and outer
medulla of the newborn kidney (Yu et al. 2002; Fabian et al. 2012).
1.3.3 Patched Receptor in Vertebrates
Patched (Ptc) in Drosophila encodes a transmembrane receptor protein containing 12
hydrophobic membrane-spanning domains, with two large hydrophilic extracellular domains
where HH ligand binding occurs (Figure 5) (Zaphiropoulos 2004). In zebrafish, mice, and
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humans two Ptc homologs (Ptc1, Ptc2) are found , both of which are direct transcriptional
targets of HH, and as in Drosophila, act to repress the HH signalling pathway (Goodrich et al.
1997; Pearse et al. 2001; Lewis et al. 1999). PTCH1 and PTCH2 proteins share 56% aminoacid
similarity, with the key difference being that PTCH2 is more stable due to a truncated C-
terminus (Holtz, K. A. Peterson, et al. 2013). While Ptc2 expression is not reported in the
embryonic kidney, Ptc1 is detected in the epithelium of the presumptive ureter and the distal
collecting ducts as early as E13.5 (Fabian et al. 2012; Cain et al. 2009). Interestingly,
unpublished data from Joshua Blake (Previous Graduate Student, Rosenblum Lab) has also
demonstrated Ptc1 expression in the capsular and cortical stroma at E15.5 in mice expressing a
Ptc1LacZ reporter.
Whether the vertebrate Ptc1 and Ptc2 genes serve distinct roles in HH regulation,
particularly during embryonic development is not fully understood. Due to the overlapping
expression of Ptc2 and Dhh in the testis, it was previously thought that PTCH2 might interact
with DHH to control germ cell development. However, germline deletion of PTCH2
demonstrated only a relatively mild phenotype, with the mutants being viable and fertile,
suggesting that PTCH2 is dispensable for embryonic development (Zaphiropoulos 2004). In
contrast, PTCH1 deletion in mice leads to embryonic lethality at E9.0-10.5, and the embryos
were found to have open and overgrown neural tubes (Nieuwenhuis et al. 2006). Interestingly,
the combined loss of PTCH1 and PTCH2 results in a yet more significant phenotype and
expansion of HH signalling activity domain, indicating that PTCH1 and PTCH2 are not
completely redundant (Holtz, K. A. Peterson, et al. 2013). Recent in vitro evidence showed that
Ptc1-null cells remain partly dependent on SHH for activation of the SHH response, supposedly
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due to SHH signalling via Ptc2 (Alfaro et al. 2014). However, there is no compelling in vivo
evidence in support of the compensatory role of Ptc2 in the absence of Ptc1.
1.3.4 Advances in Defining the Role of Hedgehog Signalling in Kidney Development
Mutations that are predicted to generate a truncated protein similar in size to GLI3
repressor have been reported in humans with Pallister-Hall Syndrome (PHS). PHS is
characterized by multiple CAKUT phenotypes with variable penetrance (Hall et al. 1980; Kang
et al. 1997). Generation of several genetically engineered mouse models has provided a strong
basis for understanding the importance of HH signalling in human diseases, particularly
congenital renal diseases. Homozygous Gli3∆699 mutant mice, which express only the truncated
form of Gli3, exhibit features similar to human PHS characterized by multi-tissue abnormalities
including renal agenesis or dysplasia, renal hypoplasia and hydronephrosis (Kang et al. 1997).
Moreover, mice with mutations in the HH signalling pathway (ie. Shh-,Gli1-,Gli2-null mice),
exhibit anomalies known to occur in VACTERL syndrome in humans characterized by
dysplastic and pelvic kidney (Kim et al. 2001). Specifically, Shh -/- kidneys are characterized by
sustained activity of GLI3-repressor and decreased levels of GLI1 and GLI2, such that upon
genetic removal of Gli3 in Shh null background, kidney induction is restored (Hu et al. 2006).
Together, these studies suggest the critical importance of SHH-GLI pathway in renal health and
disease.
A growing body of literature has provided further insight into our understanding of the
embryological mechanisms controlled by HH signalling during renal development. The spatial
restriction of Shh and its receptor, Ptc1, to the distal ureteric epithelium suggests a role for HH
signalling during branching morphogenesis. In fact, Shh mutation targeted to the ureteric lineage
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(using Hoxb7Cre) causes hydronephrosis in adults, hydroureter and hypoplasia at birth (Yu et al.
2002). Further analysis of the mutants demonstrated that SHH promotes proliferation,
establishment and maintenance of sub-epithelial ureteric mesenchyme adjacent to SHH-
producing cells of the ureter, while it inhibits smooth muscle differentiation (Yu et al. 2002).
Manipulation of HH activity through Ptc1- or Smo-deficient mice suggested that while SHH,
emitted by the collecting duct, is not required in the medullary ureteric cells themselves, the
absence of the signal is critical for proper ureteric tip-specific gene expression, and also
functions in nephron induction and branching morphogenesis (Cain et al. 2009). Moreover
conditional expression of GLI3R in nephrogenic mesenchyme and ureteric lineage using Six2Cre
and Hoxb7Cre, demonstrated a cell-autonomous role of GLI3R in controlling self-renewal of
nephron progenitors and regulating branching morphogenesis, respectively (Blake et al. 2015).
1.4 Ureteropelvic Junction (UPJ) Obstruction
1.4.1 Ureteropelvic Junction Obstruction in Humans
Effective urinary transport from the kidneys to the bladder requires connectivity between
the collecting ducts to the renal pelvis and from the pelvis to the ureter. Obstruction of the
urinary tract during fetal development causes congenital obstructive nephropathy (CON), which
is a common cause of chronic kidney disease (CKD) and end stage renal disease (ESRD) in
children. Urinary tract blockage leads to hydronephrosis, a swelling of the kidney due to
accumulated urine in the renal pelvis, which is detected by antenatal ultrasound in 1:100 to 1:500
pregnancies (Williams et al. 2007). The underlying etiologies of antenatal hydronephrosis are
diverse in nature. While the majority of cases are non-obstructive a significant proportion of
cases exhibit obstruction at the junction of the ureter and pelvis (Thom et al. 2013; Rodriguez
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2014). The gold-standard therapy for UPJ obstruction remains surgical excision of the strictured
UPJ (Klein et al. 2011; Oliveira et al. 2016). Although not all cases of UPJ obstruction require
surgical intervention, the ability to define which cases are self-resolving or will benefit from a
surgical procedure remains elusive (Oliveira et al. 2016). Moreover, severe UPJ obstruction can
result in irreversible modifications of the renal parenchyma (Zhang et al. 2000). Therefore, it is
imperative to identify predictive markers for different clinical outcomes in UPJ obstruction and
to develop curative non-invasive therapies.
1.4.2 Underlying Causes of UPJ Obstruction
Human renal biopsies of patients with intrinsic UPJ obstruction demonstrate dilation of
the proximal and distal tubules and collecting ducts, chronic tubulointerstitial injury,
glomerulosclerosis, fibrosis, and aberration of glomerular development (Ekinci et al. 2003).
Classical analyses of neonatal UPJ tissues with intrinsic obstruction, attempting to determine
developmental causes of UPJ obstruction, have associated defective UPJ with abnormal smooth
muscle arrangement. Moreover, comparison of surgically removed obstructed UPJ specimens
with autopsies from age-matched healthy cadavers revealed an increased index of smooth muscle
apoptosis and a higher level of elastin and collagen in the obstructed UPJ tissue (Reviewed in
(Williams et al. 2007)). Yet, these studies do not determine whether smooth muscle apoptosis is
primary or secondary to the obstruction. More recently, a number of genetic mutations (such as
Tbx18 and ATGR2) that are likely to cause delayed apoptosis of the undifferentiated ureteric
mesenchyme have been identified in association with UPJ obstruction (Vivante et al. 2015;
Nishimura et al. 1999).
The two mouse models of obstructive hydronephrosis published to date are both
characterized by developmental defects in urothelial and smooth muscle differentiation.
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Urothelium-specific deletion of Sec10, the central unit of exocyst complex, causes disruption of
urothelial cell differentiation and formation of the hydrophobic barrier in the ureter (Fogelgren et
al. 2015). The resulting urinary damage to ureteric mesechencymal and smooth muscle cells was
suggested to cause over proliferation of the surrounding cell population, eventually blocking
urine outflow (Fogelgren et al. 2015). In a separate study, it was demonstrated that loss of the
core component of TGFβ signalling pathway, Smad4, in the lower urinary tract mesenchyme
initially results in disruption of smooth muscle differentiation, and lack of peristalsis (Yan et al.
2014; Vrljicak et al. 2004). This functional impairment was shown to progress into a permanent
physical obstruction of the UPJ and hydronephrosis, due to a kink in the ureter and epithelial
crowding.
Unpublished work performed by Lijun Chi (Previous Fellow, Rosneblum Lab) and
Marian Staite (Previous Graduate Student, Rosenblum Lab) has uncovered a novel role of HH
signalling in UPJ development in mice. HH signalling functions in MM were investigated by
Rarb2Cre-mediated deletion of the HH cell surface receptor Ptc1 in the metanephric lineage
including stromal and nephrogenic cells. The resultant increase in HH signalling activity in the
MM produced an expanded cortical stromal domain marked by Foxd1 and Raldh2, and ectopic
expression of stromal markers at the presumptive UPJ as early as E13.5 (Figure 6A-D).
Moreover, an ectopic cell cluster expressing Ptc2, a homolog of Ptc1, causes physical
obstruction of the UPJ and marked hydronephrosis prior to birth (Figure 6E-J). Remarkably,
constitutive expression of GLI3 repressor in Ptc1-deficient background rescues stromal
expansion in the outer cortex as well as the discontinuous UPJ phenotype, indicating the
essential role of GLI3R in UPJ development (data not shown). On the contrary, Ptc2 deficiency
in Ptc1-null background does not reduce the severity of the phenotype suggesting that Ptc2 does
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not play a compensatory role to inhibit HH signalling activity. Together, these data demonstrate
that elevated HH signalling in the MM results in increased in cortical stromal gene expression
and prenatal UPJ obstruction caused by cells of stromal origin. These results raise the possibility
that HH signalling acts in the precursors of cortical stromal cells to influence their specification
and provide a basis for understanding the role of HH signalling in early lineage decisions as well
as pathogenesis of obstructive hydronephrosis.
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Figure 6 Activated HH signalling in metanephic mesenchyme affects stromal gene expression and leads to obstructive hydronephrosis
In situ hybridization demonstrates that Foxd1 and Raldh2 mRNA expression domain is broader in the
cortex of Ptc1 mutant kidneys (A-D). At E18.5, Ptc1 mutant kidneys exhibit a dilated renal pelvis and
thinning of the cortex. Intrapelvic dye injections demonstrate a blockage of flow at E18.5 compared to
WT, illustrating an absence of a patent lumen connecting the ureter and pelvis. (G-J). Beta-galactosidase
staining at E13.5 using LacZ as a reporter of Ptc2 expression in WT and Ptc1 mutant kidneys shows
ectopic Ptc2 expression between the ureter and the presumptive pelvis (E-F).
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1.5 Concluding Remarks, Hypothesis and Objectives
Stromal cells are one of the earliest lineages specified in the mammalian embryonic
kidney. Embryonic stromal progenitor cells are marked by expression of the transcription factor
Foxd1 as early as E 10.5-E11.5, and play crucial roles in normal renal development. The
published body of work strongly suggest that an Osr1+ progenitor pool derived from the T+
PSM has the potential to give rise to both stromal and nephron progenitors. Further studies have
shown the emergence of a subpopulation of Osr1+ cells expressing Tbx18 that contributes to
both ureteric mesenchyme and the renal stromal lineage. It is also likely that stromal progenitors
are a heterogeneous population of cells, a subset of which is derived from the PM. The molecular
mechanisms and signalling pathways regulating the separation of the stromal lineage from either
nephrogenic or ureteric mesenchymal lineages has remained poorly understood.
Understanding the temporal pattern of specification, and identifying novel molecular
markers of the common precursor population of the stromal and nephrogenic lineages is likely to
lead to a more comprehensive view of the origin of stromal cells. Sall1, a zinc-finger
transcription factor functioning downstream of Osr1, is highly expressed in both nephrogenic
and stromal progenitors in the developing kidney (Osafune et al. 2006a; James et al. 2006) .
Sall1 is expressed in the posterior trunk mesoderm (ie. PSM), and was recently shown to be
actively involved in the transcriptional network that regulates stem cell pluripotency during
embryoid body formation (Buck et al. 2001; Karantzali et al. 2011). Yet, whether early Sall1
expression is restricted to any of the earliest committed populations or represents common
precursors of stromal and nephrogenic lineages remains to be elucidated.
Moreover, previous work conducted in the Rosenblum lab demonstrated that
constitutively active HH signalling during renal development (through Rarb2Cre; Ptc1flox/-)
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results in an expanded stromal gene expression and prenatal UPJ obstruction caused by
ectopically located stromal cells. However, Rarb2-Cre transgenic mice were generated using a
4.8kb Rarb2 sequence fused with Cre that does not faithfully mimic the endogenous activity of
Rarb2 promoter (Kobayashi et al. 2005). Therefore, the exact origin of the stromal cell types
observed in this model remained elusive. Delineating the spatial and temporal role of HH
signalling in early renal development will help us gain further insights into the mechanisms by
which stromal versus nephrogenic cell fate decisions can be modulated, and will in turn reveal
how early activation of HH-GLI pathway leads to congenital UPJ obstruction.
Based on our previous unpublished data and the available literature, I hypothesized that
HH signalling controls the specification of nephrogenic and stromal progenitors within the
uncommitted multipotent precursor compartments marked by Osr1 and Sall1. This hypothesis is
examined here through the three objectives outlined below:
1. Determine the multipotent potential of Sall1+ cells prior to complete segregation of the
stromal and nephrogenic lineages at the onset of ureteric branching.
2. Examine the time dependent effects of activated HH signalling in Sall1+ and Osr1+ cells
on nephrogenic and stromal cell specification.
3. Define the contribution of stromal cells and HH signalling to the formation of UPJ
obstruction in mouse and human models of the disease.
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Chapter 2: Activated HH Signalling in
Multipotent Renal Precursors Promotes Stromal
Cell Specification
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2.1 Introduction
Studies have identified an abundant presence of Sall1-expressing cells in both condensing
mesenchyme, and nephrogenic structures (Nishinakamura et al. 2005). Colony formation studies
have shown that isolated E11.5 Sall1+ cells give rise to all the tubular structures of the nephron
in vitro (Osafune et al. 2006a). Although molecular markers of the stromal lineage (Foxd1 and
Raldh2) were not detected in the colonies formed, data from microarray analysis on Sall1+ cells
indicated expression of Foxd1 and Raldh2 in this population (Osafune et al. 2006b). Therefore, it
is likely that Sall1+ cells are multipotent in nature and are not fully committed to the
nephrogenic lineage prior to the onset of kidney development.
Osr1 is a definite marker of the nephrogenic mesenchyme with broader differentiation
capacity, giving rise to stromal, nephrogenic and endothelial progenitors of the kidney, as well as the
ureteric mesenchyme between E9.5-E11.5 (Mugford et al. 2008). Previous results generated by
Marian Staite demonstrated that aberrant HH activity in the MM leads to the expansion of cortical
stromal gene expression. Whether HH signalling activation specifically plays a role in perturbing the
fate of the Sall1+ cells and/or their upstream precursors to become a stromal or nephrogenic
progenitor requires further investigation.
Here I addressed the lineage of Sall1+ cells using a temporal fate-mapping approach. Cell
fate analysis reveals the contribution of Sall1+ cells at E9.5 to all the stromal and nephrogenic
cells, whereas Sall1+ cells at E10.5 are restricted to nephrogenic and capsular stromal cell fate.
Temporal activation of HH signaling in Sall1+ cells and their upstream Osr1+ cells at E9.5 leads
to an early increase in stromal cells, disorganization of the cortical stroma and ectopic presence
of cortical stromal cells in the medulla. Since there is no change in the number of nephron
progenitors or Six2 expression, our findings suggest a stage- and lineage- specific effect of HH
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signaling in promoting stromal cell specification from their common precursor cells marked by
either Sall1 or Osr1.
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2.2 Experimental Methods
2.2.1 Mouse Models
Mice used in all experiments were housed at the Toronto Center for Phenogenomics
(TCP) animal facility (Toronto, Canada). All experimental protocols using mice were approved
by the Animal Committee at TCP and were carried out in accordance with the regulations of the
Canadian Council on Animal Care (CCAC). For staging of embryos, the morning of vaginal plug
was designated as embryonic day 0.5 (E0.5). Littermates were used for all experiments in which
normal and mutant embryos were compared.
Osr1EGFPCreERt2 and Sall1CreERt2 mice were mated to Ptc1+/- mice to generate
Sall1CreERt2;Ptc1+/- or Osr1EGFPCreERt2; Ptc1+/- males, which were then crossed with homozygous
Ptc1 conditional (Ptc1flox/flox) mice to generate Sall1CreERt2;Ptc1flox/- or Osr1EGFPCreERt2;Ptc1flox/-
mutant progeny, respectively (Mugford et al. 2008; Inoue et al. 2010; Ellis et al. 2003). Ptc1 is
specifically mutated in Sall1+ or Osr1+ cells of the progeny when Cre recombinase is activated
within 24 hours after tamoxifen (TM) administration to a pregnant female. TM (Sigma) was
dissolved in Sesame oil (Sigma) and administered interaperitoneally at a single dose of
1mg/40mg body weight at noon of the desired embryonic day.
Analysis of HH activity was achieved by mating Sall1CreERt2 mice to Ptc1LacZ/+ mice to
generate Sall1CreERt2; Ptc1LacZ/+ males (Goodrich et al. 1997). These males were crossed with
homozygous Ptc1 conditional mice to generate Sall1CreERt2;Ptc1LacZ/flox mutant progeny carrying a
LacZ reporter allele. Cre-dependent recombination is activated in Sall1+ cells upon TM injection
according to the above procedure.
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Temporal fate mapping was performed by crossing the R26-tdTomato reporter strain with
Sall1CreERt2 mice. tdTomato heterozygote progeny express a bright red fluorescence protein (RFP)
upon TM-induced Cre-recombination (Kobayashi et al. 2012).
Table 1. List of primers for PCR analysis of mouse genomic day
2.2.2 Whole Mount B-Galactosidase Staining
Whole kidneys were briefly fixed in lacZ fix solution (25% gluteraldehyde, 100 nM EGTA,
1 M MgCl2, 0.1 M sodium phosphate) and rinsed in wash buffer (0.1 M sodium phosphate buffer,
2% nonidet-P40), 1 M MgCl2). Kidneys were then placed in lacZ staining solution (25 mg/ml X-
gal, potassium ferrocyanide, potassium ferricyanide) at 37 ͦ C overnight in the dark. Once staining
had occurred, the reaction was terminated in wash buffer and tissue was post-fixed in 10% buffered
formalin at 4 ͦ C. Stained tissues were then processed for embedding in paraffin wax followed by
midsagittal sectioning at intervals of 5 µm. Sections were then counterstained with eosin.
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2.2.3 Histology and Immunohistochemistry
To harvest embryos from timed pregnancies, pregnant mice were sacrificed using a CO2
chamber. Embryonic tissues were fixed in 4% Paraformaldehyde (PFA) at 4 ͦ C overnight, and
were dehydrated in 70% ethanol prior to paraffin embedding and further processing. Midsagittal
tissues sections were generated in 5µm intervals by the CMHD’s Core Pathology Lab at TCP,
followed by hematoxylin and eosin (H&E) staining using standard methods.
To visualize tdTomato expression, fixed kidneys were cryopreserved using a sucrose
gradient (gradual increase from 10 to 30%), embedded in OCT (Sakura Finetek, Torrance, CA),
and sectioned at 8 µm. Cryosectioning of the OCT embedded tissue was conducted by the TCP.
Immunofluorescence staining was carried out on either paraffin embedded or frozen
sections according to the following procedure. Sections were first deparaffinised in xylene and
rehydrated through ethanol gradient. Subsequently, antigen retrieval was performed on paraffin-
embedded tissues using citrate buffer (pH=6) in a pressure cooker. Non-specific antigens were
blocked at room temperature using DAKO serum-free protein block prior to incubation with
primary antibodies. Primary antibodies were diluted in protein block (DAKO) and incubated
overnight at 4o C. Primary antibodies were: R&D Mouse monocolonal anti-SALL1 (PP-K9814-
002), Abcam Rabbit polycolonal anti-SALL1 (ab31526), Cell Signalling Rabbit polycolonal
anti-PBX1 (4342), Santa Cruz Mouse anti-PBX1 (sc-889x), Abcam Rabbit polycolonal anti-
RALDH2 (ab96060), Rockland Rabbit polycolonal anti-RFP (600-401-379), Cell Signalling
mouse monocolonal anti- PHH3 (Ser10) (9706), Sigma mouse monocolonal anti- NCAM
(C9672), Proteintech rabbit polyclonal anti-SIX2 (11562-1-AP), and Dako mouse monocolonal
anti- WT1 (Clone 6F-H2). Secondary antibodies used for immunofluorescence application were
Alexa-Fluor 488 goat anti-mouse and Alexa-Fluor 568 goat anti-rabbit (Molecular Probes,
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1:1000 dilution). Nuclei were visualized using DAPI counterstain (Life Technologies, 1:2000).
Slides were mounted using liquid mounting medium (Vectashield) and imaged using an
epifluorescence microscope (Zeiss). Images were captured at the same exposure for all samples,
and 3 sections per kidney were used in the assessments.
2.2.4 In situ mRNA Hybridization
In situ hybridizations were performed on paraffin embedded embryonic sections using
digitoxin-labelled RNA probes encoding Ptc1 as described (Mo et al. 1997).
2.2.5 RNA Isolation and Quantitative Real-Time Reverse Transcriptase- PCR
To evaluate Cre-recombinase efficiency after TM administration, forelimb tissues were
harvested from embryos and preserved in RNAlater® (Sigma) at 4 ͦ C. RNA was isolated from
the ruptured tissue using a RNeasy micro kit (Qiagen). cDNA was subsequently synthesized
from the extracted RNA using oligo-dT primers and superscript(II) (Invitrogen). Real-time PCR
reaction mix contained 1ng of cDNA sample, SYBR green master mix (Invitrogen) and primers
to a total volume of 10 µl. All primers were designed using Primer Blast and verified through
UCSC in silico PCR (Table 2). Quantitative real-time PCR (qRT-PCR) amplification was carried
out using the Applied Biosystems 7900 HT fast RT-PCR system. Relative levels of mRNA
expression were determined using the ΔΔCT method, where individual expression values were
normalized to Gapdh.
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Table 2 Primer sequences used for qRT-PCR (Mus-musculus genes)
2.2.6 Flow Cytometry
To analyse gene profiles of Osr1+ cells in a state of increased HH signalling,
Osr1EGFPCreERt2 transgenic mice was used to isolate GFP+ cells via fluorescence activated cell
sorting (FACS). Osr1+ specific conditional knock outs (Osr1EGFPCreERt2; Ptc1flox/-) and
heterozygote littermate controls (Osr1EGFPCreERt2; Ptc1flox/+) were collected at E11.5 and
processed individually for FACS as below. Isolated metanephric rudiments were dissected in
HBSS:BSA and digested in 2 mg/mL of Collagenase (Roche) in DEPC/Ringer’s solution for 15
minutes at 37°C. Kidneys were dissociated into a single-cell suspension by triturating the sample
with a 1mL pipette. After centrifuging the cell suspension for 5 minutes at 4000rpm, cells were
re-suspended in sorting media (HBSS/BSA containing 1% heat inactivated FCS and 1 mM
EDTA). The cell suspension was filtered through a 40 μm nylon mesh cell strainer (BD Falcon)
into 5mL polystyrene round bottom tubes (Falcon). Propidium Iodide (Sigma) was added at a
final concentration of 0.5mg/mL to label dead cells. FACS analysis was carried out using the
FACS Vantage (Becton Dickinson Immunocytometry Systems) in the flow cytometry facility at
the Sickkids Research Institute. The gate for the Osr1-GFP+ population was set to exclude all
the cells falling into the fluorescing range of the negative control cells and to include only the
highly fluorescing population. Cells were directly collected in 350 µL of buffer RLT (Qiagen)
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and frozen on dry-ice. RNA extraction from sorted cells was performed according to the
RNeasy® Micro-kit instructions (Qiagen).
2.2.7 Automated Cell Quantification in Tissue Sections
To quantitate the number of cells, ImageJ® “analyze particles” command was used
according to published protocols(Gering et al. 2004). In summary, the image was first converted
to 8-bit black and white, followed by threshold level adjustment to mark only the cells to be
counted. The optimal size of the particles to be counted was set to be 0.01 to infinity, with no
preference for shape, and the counted particles were outlined with dashes. The area of the kidney
section was measured by ImageJ® and the total number of cells in each section was normalized to
the area of the corresponding section prior to any comparison. Wherever only the cortical stromal
or nephrogenic cells were counted, the number of cells was normalized to the number of UB tips
in the cortex rather than the area of the whole section.
2.2.8 Data Analysis
All statistical and mathematical analyses of data were done using Microsoft Excel.
Student’s t-test (with unequal variances) was used to determine the statistical significance of any
changes observed. A p-value of less than 0.05 was considered statistically significant.
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2.3 Recombination in Sall1CreERt2 Mice is Tamoxifen Dependent and Specific
to Sall1+ Cells
I first confirmed that without TM, there is no leakiness of Cre activation by assessing
tdTomato expression in Sall1CreERt2;R26-tdTomato mice that were not exposed to TM at any time
in their development (data not shown). In the developing kidney, Sall1 has been shown to be
expressed in multipotent nephron progenitor cells and CM-derived differentiating structures
(PTA, RV, comma and S- shaped bodies), as well as the cortical stromal cells (Osafune et al.
2006a; Ohmori et al. 2015). I confirmed Cre specificity in Sall1CreERt2;R26-tdTomato by
demonstrating that when TM is injected after the onset of ureteric branching (E11.5), tdTomato
expression is detected in nephron progenitors, nephrogenic intermediates and the cortical stroma,
but not in the epithelial UB-derived cells (Figure 7).
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Figure 7 Recombination Specificity in Sall1CreERt2 Mice
A-B. Immunostaining for tdTomato (red) and Sall1 (green) expression in E16.5 Sall1CreERt2;tdTomato
kidneys upon tamoxifen treatment at E11.5. C. Merged image showing yellowish colour in cells co-
expressing Sall1 and tdTomato. Inset shows the outlined region in higher magnification showing the
overlap of tdTomato expression with that of Sall1, including in the cap mesenchyme (CM), intermediate
nephrogenic structures, such as S-shaped nephrons, and cortical stroma (CS). White arrowheads point to
the Sall1+ cells in the CM and the intermediate nephrogenic structures that are not marked by tdTomato.
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2.4 Sall1+ Cells in the Caudal IM Contribute to Stromal and Nephrogenic
Lineages
Previous lineage tracing studies have demonstrated the multipotent capacity of Osr1+
cells between E7.5 and E10.5 to give rise to all the cortical progenitor compartments of the
metanephric kidney, including both stromal and nephron progenitors (Mugford et al. 2008). Osr1
has been shown to be functionally upstream of a number of genes required for initial MM
establishment including Sall1 (James et al. 2006). In the absence of Sall1, initial ureteric
branching fails to occur and the MM undergoes apoptosis (Nishinakamura et al. 2001;
Nishinakamura et al. 2005). Due to the expression of Sall1 in both nephrogenic and stromal
progenitors in the developing kidney, Sall1 expression prior to the onset of UB induction is
likely to mark the precursor of these two lineages. I addressed this hypothesis, and determined
the developmental time window during which Sall1+ cells have multipotent capacity, using a
temporal fate mapping approach. I inter-crossed Sall1CreERt2 with R26-tdTomato mice to
permanently label descendants of the Sall1+ population via TM (1mg) –mediated induction of
Cre recombination (Kobayashi et al. 2008). I traced the fate of Sall1+ cells marked at 24-hour
intervals from E9.5 through E11.5.
I first marked Sall1+ cells by injecting TM at E9.5, and subsequent analysis was
performed at E14.5-E16.5 –a stage at which full spectrum of nephrgenic and stromal cells are
developed to detect the cell types that are descended from Sall1+ cells marked at E9.5-E10.5.
TdTomato-labelled cells were found in both cortex and medulla. Immunostaining for Pbx1,
which has a low expression in nephron progenitors and high expression in stromal cells
(Schnabel et al. 2003), demonstrated that nephron progenitors and their derivatives, including
podocytes and tubular structures, were tdTomato+ (Figure 8A-C). In addition, tdTomato
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expression co-localized with cortical stromal cells marked by high expression of Pbx1 (Figure
8A-C). I also found traces of tdTomato expression in the medullary stroma intercalating the renal
tubules and collecting ducts (Figure 8A-C). These data suggest that Sall1+ cells between E9.5
and E10.5 have the potential to contribute to the stromal progenitor compartment in addition to
the nephrogenic lineage.
Surprisingly, when TM was injected at E10.5, tdTomato+ cells were exclusively found in
the CM surrounding the UB tips, and the flat layer of capsular stroma (Figure 8D,white
arrowheads). No tdTomato expression was detected in other cortical and medullary stromal cells
(Figure 8D, white arrows). Together, the results of our lineage tracing experiments suggest a
gradual restriction in the fate of Sall1+ precursors between E9.5 and the onset of ureteric
branching at E11.5, such that these cells initially have the capacity to contribute to both
nephrogenic and stromal lineages, but lose their ability to contribute to the majority of stromal
cells (except for the capsular stroma) at E10.5.
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Figure 8 Sall1+ cells transiently contribute to cortical stromal cells between E9.5 and E10.5
A-C. Immunostaining of E16.5 Sall1CreERt2;tdTomato kidneys for tdTomato and Pbx1 after Cre activation
at E9.5. Low Pbx1 expression marks nephron progenitors (yellow arrows). tdTomato expression co-
localizes with Pbx1 expression, in the capsule, sub-capsular stroma and the medullary stroma (white
arrowheads) upon tamoxifen administration at E9.5 (n=4). D-F. Immunostaining of E16.5
Sall1CreERt2;tdTomato kidneys for tdTomato and Pbx1 after Cre activation at E10.5. tdTomato expression
is found in the nephron progenitors and their derivatives. Pbx1 expression colocalizes with tdTomato
expression only a subset of cells in the inner layer of capsular stroma (arrows). There is no tdTomato
expression in the medullary stroma and the cortical stromal cells in between nephron progenitors
(asterisks) (n=3).
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2.5 Increased Hedgehog Signalling in Sall1+ Cells at E9.5
To investigate the role of increased HH signalling in lineage specification within Sall1+
cells I used Sall1CreERt2;Ptc1+/- mice and crossed them with Ptc1flox/flox mice to conditionally
delete Ptc1 in a temporally controlled fashion between E9.5 and E10.5. Following loxP site
recombination, Exon 3 of the Ptc1 gene is excised to generate a non-functional PTC1 protein
(Ellis et al. 2003). Since Sall1 is also strongly expressed in the developing limb bud (Buck et al.
2001), I used RNA extracted from the limb tissue of each embryo to confirm that a single dose
TM injection activated Cre recombination. Results of qRT-PCR analysis using Ptc1 primers
designed for the disrupted region of Ptc1 demonstrated a 50% decrease in Ptc1 mRNA in E13.5
mutants (Sall1CreERt2;Ptc1flox/-) compared to the heterozygote controls after TM injection at E9.5
(Figure 9B). Next, I confirmed that Ptc1 inactivation in Sall1+ precursor cells results in
increased HH signalling activity in mutant mice by analyzing Ptc1-lacZ expression as a readout
of HH signalling. In the embryonic kidney, Ptc1 is normally expressed in the mesenchyme
surrounding the presumptive ureter, and the urothelium. Up-regulated Ptc1 mRNA expression as
a consequence of activated HH signalling in Sall1+ precursor cells is predicted to result in
ectopic lacZ expression in the cortex. Our data confirmed that while Ptc1-lacZ expression was
maintained in the urothelium and medullary stroma (similar to controls), it was ectopically
expressed, albeit in a mosaic pattern, in the condensing mesenchyme and the stroma surrounding
the UB tips in mutant kidney tissue (Figure 9C-F).
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Figure 9 Schematic of Ptc1 gene and confirmation of Ptc1 deletion and HH activity
A. Schematic of murine Ptc1 gene demonstrating the location of loxP allele in relation to Exon 3. B. At
E3.5 kidneys were dissected from ureteric tissue after tamoxifen administration at E9.5, and mRNA levels
of the excised region of Ptc1, exon3, were measured. Ptc1-mutant kidneys exhibited a significant
decrease in Ptc1 (50%) compared to the heterozygote controls. C-F. β-galactosidase staining at E13.5 and
E16.5 for LacZ as a reporter of Ptc1 expression shows persistant HH activity in the medullary stroma
around the ureter and ectopic activity in the cortex, compared to control expression only in the medullary
stroma surrounding the ureter.
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Histological examination followed by TAM administration at E9.5 did not show a
marked phenotypical difference in mutant kidneys compared to the wild-type littermate controls
at E13.5. However, immunostaining for CM marked by Sall1, and cortical stroma marked by co-
expression of Pbx1 and Sall1, revealed an expansion of Sall1+ cells and aberrant localization of
cortical stromal cells at E14.5 (Figure 10A,B). In wild-type kidneys, cortical stromal cells are
arranged in a very organized pattern consisting of a layer of capsular stroma and 1-2 layers of
sub-capsular cells (Figure 10A). In contrast, in mutant mice some stromal cells surrounded the
CM in an unusual multi-layer pattern, and some formed a single layer in between multiple Sall1+
cell layers (Figure 10B). To further investigate the disrupted arrangement of cortical stromal
cells, I examined the expression of Raldh2, a specific marker of cortical stromal cells through
immunostaining. In mutant kidneys, Raldh2 was expressed ectopically in the junction of the
ureter and pelvis, an area normally occupied by the medullary interstitial cells at both E13.5 and
E16.5 (Figure 10C-E). These data raise the possibility that the disrupted arrangement and ectopic
localization of cortical stromal cells may be due to abnormal expansion of stromal progenitors.
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Figure 10 Ptc1 mutant kidneys display disorganized cortical stroma
Ptc1 mutant kidneys display disruption in arrangement of the cortical stromal cells marked with Pbx1
(red) and Sall1 (green) compared to the typical 1-2 organized cell layers around the CM. Insets show the
higher magnification of the dashed areas (A-B).Raldh2, a specific marker of cortical stromal cells is
ectopically expressed at the junction of the ureter and the presumptive pelvis at both E16.5 (C-D) and
E13.5 (E-F).
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To determine whether there is a difference in the number of stromal cells in mutant
kidneys, three midsagittal sections (80 μm apart), from four different E13.5 mutants and
littermate controls, were immunostained to mark stromal cells by high Pbx1 expression (Pbx1Hi)
(Figure 11A,B). Low Pbx1 expression marks the nephron progenitor population. Next, the
average number of Pbx1Hi cells across different kidney layers were counted using ImageJ®
software. This analysis indicated a 78% increase in the total number of Pbx1+ cells, including
the cortical and medullary interstitial cells, per unit area in Sall1CreERt2;Ptc1flox/- kidneys (n=4,
p<0.005) (Figure 11C). Next, I examined the number of stromal progenitors, by exclusively
counting Pbx1+ cells in the renal cortex, including the capsular and sub-capsular stroma, and the
cells intercalating the UB tips. The number of cortical stromal cells per UB tip was found to be
increased by 20% in the mutants (n=4, p<0.05) (Figure 11D). Taken together, our quantitative
analyses demonstrated that HH signalling activation in Sall1+ precursors at E9.5 leads to a
significant increase in the number of stromal progenitors and their derived medullary stromal
cells at E13.5.
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Figure 11 Increased number of cortical and medullary stromal cells in Ptc1 mutant kidneys
A-B. Immunostaining of E13.5 kidneys for stromal cells marked by Pbx1 expression (dashed area marks
renal cortex). Both cortical and medullary zones, separated by dashed line, are populated with more
Pbx1Hi cells in mutants. C. Quantitation of the number of Pbx1Hi stromal cells in sections across the
kidney shows a 78% increase in total number of stromal cells per unit area in mutants compared to
controls. (n=4, P<.005). D. Quantitation of the number of Pbx1Hi stromal cells exclusively in the cortex
(outlined area), reveals a modest but significant increase in the number of stromal progenitors in mutants
compared to controls (n=4, P<0.05). CS=Cortical Stroma, MS=Medullary Stroma, UB=Ureteric Bud.
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Next, I examined whether the increased number of stromal cells in mutant kidneys is
caused by increased cell proliferation in the stromal compartment, since HH signalling was
previously shown to promote proliferation of the ureteric and medullary mesenchyme (Yu et al.
2002). I used two different cell cycle markers, PHH3 and Ki-67, to mark proliferating cells at
E13.5, when an increase in stromal cells is observed (Figure 12A-E). Ki-67 marks cells in all
active phases of cell cycle, whereas PHH3 specifically marks mitotic cells in G2-M phase at the
time of analysis. Statistical analysis of the proportion of Pbx1+ cells expressing Ki-67, or PHH3
per unit area did not indicate a significant difference between mutants and controls (n=4). These
results indicate that Pbx1+ stromal cells do not undergo increased proliferation in mutant
kidneys, and that increased cell proliferation does not underlie the expansion of stromal cells
observed in the mutants.
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Figure 12 Proliferative capacity of Pbx1+ cells is not changed in Ptc1 mutants at E13.5
Cell proliferation was measured by immunostaining for PBX1, co-localizing with Ki-67, a specific
nuclear marker for proliferating cells (red) (A-B), or PHH3 (red) (D-E). Quantitation of both markers
shows that proliferative index in mutant stromal cells is comparable to that of the WT controls (C-F).
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In summary, our data showed that activation of HH signalling in Sall1+ cells at E9.5
leads to an increased number of stromal progenitors, disorganization of the progenitor
compartments (nephrogenic and stromal) around the UB tips, and ectopic localization of the
cortical stromal cells in the medullary kidney. At the same time, the Sall1CreERt2; Ptc1flox/-model
has limitations since the earliest stage when immunofluorescence staining allows for reliable
quantitation of the number of Pbx1Hi cells across serial sections is E13.5. At E13.5, stromal
progenitors have already started to contribute to differentiated stromal derivatives. Thus, the
magntitude of the effect of HH signalling activation in Sall1+ precursors on initial stromal
progenitor specification may have been masked. Also, the fact that Sall1+ cells in Sall1CreERt2;
Ptc1flox/- mice are not marked with a reporter allele in this model hinders our ability to examine
whether the ectopic stromal cells descend from the precursor population within which HH
signalling was activated. It could be possible that non-cell autonomous effects of HH signalling
perturb the fate of the surrounding Sall1-negative cells such that they gain stromal
characteristics.
2.6 Increased Hedgehog Signalling in Osr1+ Cells at E9.5
Osr1 expression has been shown previously to mark multipotent renal progenitors, whose
developmental potential is less restricted than that of Sall1+ cells, and can give rise to nephrons,
stroma and capillary endothelial cells between E9.5 to E11.5 (Mugford et al. 2008). To address
the cell-autonomous function of activated HH signalling within the common precursor of the
nephrogenic and stromal lineages, I took advantage of endogenous GFP expression in
Osr1EGFPCreERt2 mouse strain, and continuous Osr1 expression in the IM, stromal and
nephrogenic progenitors between E9.5- E11.5 (Mugford et al. 2008). Fluorescence-activated cell
sorting (FACS) was used to isolate Osr1+ cells from E11.5 Osr1EGFPCreERt2;Ptc1flox/- and
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Osr1EGFPCreERt2;Ptc1flox/+ (Ptc1 heterozygote control) MM after TM administration at E9.5
(Figure 13A). This allowed us to quantitatively measure the contribution of activated HH
signalling in promoting specification of stromal progenitors from their Osr1+ precursor
population at the onset of renal development.
After optimization of flow-sort conditions, sample collection and subsequent RNA
extraction from small cell volumes, I isolated an average of ~13,800 GFP+ MM cells per pair of
E11.5 metanephric rudiment (Figure 13B). qRT-PCR analysis was performed to confirm the
relative purity of the FACS-isolated Osr1-GFP+ population by comparing Osr1 mRNA levels in
GFP+ to that in the GFP- cell fraction. Results of our analysis on 10 pairs of metanephroi
demonstrated 12-fold greater Osr1 expression in GFP+ cells compared to the GFP- cell fraction
(Figure 13C). I subsequently confirmed that Cre-induced recombination resulted in deletion of
Ptc1 (73% decrease) in Osr1EGFPCreERt2;Ptc1flox/- cells using qRT-PCR with primers specific for
the loxP-flanked exon 3 of the Ptc1 gene (Figure 13D). Next, I compared the mRNA expression
levels of stromal and nephron progenitors specific markers, Foxd1 and Six2 respectively, in
control (Ptc1 heterozygous) and Ptc1-null Osr1+ cells. qRT-PCR analyses demonstrated a 60%
increase in Foxd1 expression in the Ptc1-null Osr1+ cells (n=5, p<0.05), while Six2 expression
remained relatively unchanged (Figure 13E,F). These data in conjunction with our previous
findings from Sall1CreERt2 mice suggest that HH signalling activation within the common
precursors of stromal and nephrogenic cells at E9.5 promotes specification of the stromal cell
lineage.
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Figure 13 Foxd1 is increased in Ptc1 null Osr1+ cells
A. The inset shows a representative Osr1EGFPCreERt2+ metanephros at E11.5, where GFP expression marks
Osr1+ cells. The plot represents FACS analysis of GFP labeled Osr1+ MM cells B. The table represents
the average number of Osr1+ cells sorted from 10 pairs of metanephroi (5 mutants and 5 controls). C.
Quantitative real-time PCR (qRT-PCR) of E11.5 FACS-isolated cells confirmed significantly higher
expression of Osr1 in GFP+ compared to GFP- cell fractions. D. qRT-PCR analysis showed a 70%
decrease in Ptc1 exon3 of mutant Osr1+ cells compared to controls, confirming Cre dependent
recombination upon tamoxifen administration at E9.5. E-F. Foxd1 expression is increased by 60% in Ptc1
null Osr1+ cells, whereas Six2 expression is relatively unchanged in mutant and control cells.
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To investigate how the increase in stromal gene expression influences development of the
kidney in Osr1EGFPCreERt2;Ptc1-null mice, I examined stromal and nephrogenic progenitors using
immunostaining at advancing stages in development (Figure 14 A-H). Consistent with our
finding in Sall1CreERt2;Ptc1-null kidneys, immunostaining for nephron progenitors marked by
Sall1, and cortical stroma marked by co-expression of Pbx1 and Sall1, revealed an expansion of
Sall1+ cells and abnormal localization of cortical stromal cells at E14.5 in mutants (Figure 14B).
In addition to the ectopic Raldh2+ cells in the medullary region similar to Sall1CreERt2; Ptc1-null
mutants, E16.5 kidneys exhibited an ectopic population of Raldh2+ cells within the ureteric
lumen, more distal to the kidney (Figure 14C,D). Previous studies have suggested the
establishment of a Tbx18+ population within the Osr1+ metanephric field at E10.5, that lies in
close proximity to the ND, and contains both ureteric mesenchyme and renal stromal
progenitors, distinct from the already specified Foxd1+ cells (Bohnenpoll et al. 2013a). It is
likely that aberrant activation of HH signalling in Osr1+ cells impact the fate of Tbx18+ cells to
further contribute to the stromal progenitor population in the kidney-proper/ureter.
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Figure 14 Stromal progenitor marker, Raldh2, is ectopically expressed in Osr1EGFPCreERt2; Ptc1-null mutants
A. Histological comparison of E16.5 mutant (Osr1CreErt2;Ptc1 flox/-) with control (Ptc1 flox/+) shows
the onset of renal pelvis and tubular dilation in addition to the aberrant presence of cell clusters
inside the ureteral lumen (black arrows). B. Immunostaining of E14.5 kidneys shows disruption in
regular organization of nephron progenitors, marked by Sall1 (green) and stromal cells, marked by
Sall1 and and Pbx1 (yellow) around the UB tips in mutants compared to controls. C.
Immunostaining for Raldh2 (red) shows ectopic expression in the medullary stroma in E15.5
mutants (dashed circles). D. Immunostaining of E16.5 kidneys demonstrates that the cell clusters
inside the ureteral lumen also display Raldh2 (red) expression (white arrow).
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2.7 Defect in Nephrogenesis in Ptc1 Mutant Kidneys
Various studies have established a molecular signalling crosstalk between cortical
stromal cells and nephron progenitors, important for regulating nephron number(reviewed in (Li
et al. 2014)). Since lineage tracing results showed that Sall1+ cells in the IM and un-induced
MM contribute to both stromal cells and nephron progenitors, I investigated how HH signalling
activation in Sall1+ cells at E9.5 affects nephron progenitors and their differentiation.
Analysis of nephron number, marked by WT1 immunostaining, revealed a 24 % decrease
in the number of mature glomeruli in Sall1CreERt2;Ptc1flox/- mutant kidneys compared to littermate
controls (n=4, p<0.01) (Figure15). To investigate the underlying mechanism, I first determined
whether activated HH signalling in Sall1+ progenitors leads to an increase in the number of
stromal progenitors with the concomitant negative effect on nephron progenitors. Quantitation of
the number of Six2+ cells surrounding each UB tip demonstrated no significant difference
between the mutants and controls at E13.5, the stage at which I measured 20% increase in
cortical stromal cells (Figure 16A-C). This finding is consistent with the qRT-PCR analysis of
Osr1+ cells FACS-isolated at E11.5, which demonstrated no significant difference in Six2
expression in mutant and heterozygote controls (Figure 13F). Next, I examined the patterning of
the CM surrounding UB tips at E18.5 through immunofluorescence staining of PAX2 and SALL,
additional markers of nephron progenitors. All the 5 E18.5 mutants exhibited a continuous CM
along the outer cortex comparable to the CM arrangement in WT kidneys (Figure 16D-G). These
data suggest that the lower number of mature glomeruli measured at birth are likely not the result
of defects in nephron progenitor specification or maintenance.
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Figure 15 Ptc1 mutant kidneys demonstrate a significant decrease in the number of glomeruli at E18.5.
A,B. Immunostaining of E18.5 kidneys for WT1 (green) marking mature glomeruli (grey arrows). C.
Quantification of the number of WT1+ structures demonstrates a significant decrease in the number of
glomeruli in Ptc1 mutant kidneys (B) compared to controls (A).
Figure 16 Ptc1 mutant kidneys exhibit no marked defect in nephron progenitor specification and maintenance
A,B. SIX2 staining of the nephron progenitors does not show any marked defect in nephron progenitor
domain at E13.5. C. Quantitation of the Six2+ cells confirms that the number of nephron progenitors per
UB tip in mutants is comparable to controls (n=4, p>0.05). D-G. Staining with PAX2 and SALL1 shows
an apparently normal-sized nephron progenitor compartment (arrowheads) later in development (E18.5)
(n=5).
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To determine whether reduction in glomeruli number is due to a defect in nephron
formation, I quantified the number of developing nephrons marked by NCAM immunostaining
at E13.5. Interestingly, our analysis demonstrated a 28% decrease in the intermediate
nephrogenic structures in mutants (n=5, p<0.005) (Figure 17), which closely corresponds to the
observed 24% decrease in mature glomeruli at E18.5 (reported above). A growing body of
literature as well as unpublished studies by Winny Li, Hovhannes Martyrosian and Chris Rowan
from the Rosenblum lab have established the non-cell autonomous effects of stromal population
on the MET process and nephron differentiation (Mao et al. 2015; Bagherie-Lachidan,
Reginensi, Zaveri, et al. 2015; Fetting et al. 2014; Phua et al. 2015). Thus, it is likely that
perturbations of the stromal lineage discussed above are the underlying cause of the observed
defects in nephrogenesis.
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Figure 17 Nephron formation is modestly decreased in Ptc1 mutant kidneys
A, B. Immunostaining of E13.5 kidneys for NCAM (green) marking the intermediate nephrogenic structures including, renal vesicles, comma-shaped and S-shaped nephrons. C. Quantitation of the NCAM+ structures reveals a modest (28%) but significant decrease in the number of nephrogenic intermediate structures per UB tip.
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2.8 Discussion
The majority of adult kidney cellular components are derived from two distinct self-
renewing progenitors: the stromal and nephron progenitors. Although these two populations are
spatially and molecularly distinct at the onset of metanephric development, they arise from a
common Osr1+ precursor population. Yet, labeling studies have suggested that the Osr1+ IM
mesenchyme is predominantly a heterogeneous population of cells already committed to either
cap mesenchyme or interstitial progenitor cell. Other lineage tracing studies have indicated that
Eya1+ cells contribute only to the metanephric nephron-forming cell fate, but not stroma as early
as E8.75 (Jinshu Xu et al. 2014). Despite the in vivo and in vitro advances made in understanding
the signalling mechanisms involved in establishing the nephron progenitor compartment, the
regulation of stromal cell fate from the Osr1+ progenitor pool has remained unknown (Taguchi
et al. 2014). Neither Osr1 nor Foxd1 expression are required for specification of stromal
progenitors (Yallowitz et al. 2011; Hum et al. 2014). Thus, identification of other genes marking
the common precursor population and a detailed understanding of temporal fate restriction in
precursors of the stromal and nephron progenitors will allow deeper insight the origin of stromal
cells.
2.8.1 Sall1 is expressed in the Precursors of Stromal and Nephron progenitors Between
E9.5 and E10.5.
Results of our lineage tracing experiments suggest a gradual restriction of Sall1
expression in renal precursors between E9.5 and the onset of ureteric branching at E11.5, such
that Sall1+ cells initially have the capacity to contribute to both nephrogenic and stromal
lineages, but lose their capacity to contribute to the majority of stromal cells (except for the
capsular stroma) at E10.5. It is likely that Sall1 expression is transiently turned off in the
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majority of stromal progenitors and their precursors between E10.5-E11.5, and is maintained
only in the progenitors of the renal capsule, supporting the idea that stromal progenitors are a
heterogeneous population of cells with distinct differentiation capacities, previously proposed by
Kobayashi et al (Kobayashi et al. 2014). These data also suggest a potential temporally-specific
role for Sall1 in development of the renal capsule, upstream of Foxd1 and Hoxd11. This can be
examined in more detail using Sall1CreERt2/flox mice upon TM injection at E10.5 (Levinson et al.
2005; Yallowitz et al. 2011; Inoue et al. 2010).
Moreover, SALL1 in the nephrogenic mesenchyme has previously been shown to be
involved in initial UB outgrowth (E10.5-E11.5) possibly through binding to a nucleosome
remodeling and deacetylase (NuRD) complex, and repressing unknown inhibitors of UB
outgrowth (Nishinakamura et al. 2001; Kiefer et al. 2010). Our findings raise the possibility that
lack of Sall1 expression in the precursors of stromal cells between E10.5 and E11.5 may be
required for disinhibition of genes involved in establishing the initial signalling interaction
between the MM and the growing UB. Overexpressing Sall1 in Sall1+ cells at E10.5 using
R26Sall1 mice, which allow exogenous Sall1 expression in a Cre-dependent manner, will be of
interest for future investigation (Jiang et al. 2010).
2.8.2 Activation of HH Signalling in Precursors of Stromal and Nephron Progenitors
Promotes Stromal Cell Specification
The requirement for HH signalling during murine kidney development has previously
been established by analyzing the renal phenotype in Shh-null mice, 50% of which display
bilateral renal aplasia as early as E14.5 (Hu et al. 2004). Moreover, Marian Staite previously
showed the importance of the SHH effector protein, GLI3R in regulating stromal progenitors
gene expression. To specifically address the role of HH signalling in early precursors of stromal
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progenitors, I first investigated whether constitutively active HH signalling (via Ptc1 deletion) in
Sall1+ cells had an effect on stromal and nephrogenic progenitor populations. Activation of HH
signalling at E9.5, a developmental stage prior to emergence of Foxd1+ cells anterior to the MM
(Yallowitz et al. 2011), led to disorganization of the cortical stroma at E14.5 and ectopic
expression of Raldh2, a specific marker of cortical stromal cells, at the junction of the ureter and
the presumptive pelvis at E16.5. This finding led us to examine earlier developmental stages for
the possibility of an abnormally higher number of stromal cells in the mutants. Results of our cell
quantitation analysis demonstrated a significant increase in the number of cortical stromal cells,
containing self-renewing stromal progenitors, as well as the total number of stromal cells marked
by Pbx1. Proliferation assays (using PHH3 and Ki-67 staining) confirmed that the increase in
stromal cells is not a consequence of increased proliferation in these cells. However, the earliest
stage at which the number of stromal cells and stromal progenitors can be reliably examined in
serial sections through immunostaining with Pbx1, without encountering sectioning bias, is
E13.5. Since stromal progenitors at this stage have already began to differentiate to their
derivative components our results may not truly reflect the change in stromal progenitors caused
by activation of HH signalling in their precursors at E9.5. Moreover, using Sall1CreERt2, it is not
possible to examine whether the extra stromal cells in the mutants originate from Sall1+
precursor cells with activated HH signalling. It is possible that paracrine effects of HH signalling
perturb the fate of a Sall1-negative population such that it gains stromal characteristics and
integrate into the developing kidney.
Previous lineage tracing and gene expression analysis has demonstrated Osr1 expression
in both stromal and nephrogenic progenitors at E11.5, after which Osr1 is exclusively expressed
in the nephrogenic lineage (Mugford et al. 2008). I took advantage of GFP expression in Osr1+
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cells of the Osr1EGFPCreERt2 mice to address the cell-autonomous effect of constitutively active
HH signalling on specification of Foxd1+ stromal progenitors from Osr1+ precursors. In support
of our previous findings, qRT-PCR analysis of the FACS isolated GFP-tagged Osr1+ cells at
E11.5 demonstrated a significant increase in expression of Foxd1 in Osr1+ cells in an activated
state of HH signalling. Moreover Osr1EGFPCreERt2;Ptc1flox/- mutants exhibited disorganization of
cortical stroma and ectopic Raldh2+ cells in the medulla and the presumptive UPJ area at later
stages of development. These data suggest that the increased Foxd1 expression in Osr1+ cells at
E11.5 is reflective of an aberrant increase in stromal progenitor cells caused by HH signalling
activation in either Osr1 or Sall1+ precursor cells. Analyzing the number of cells in the stromal
fraction isolated from E11.5 Osr1+ cells would add further validation to our findings (Ohmori et
al. 2015). .
2.8.3 Nephron Progenitor Specification and Maintenance are not affected by Induction of
Hedgehog Signalling Activity at E9.5
In addition to defects in arrangement and organization of stromal progenitors in the
nephrogenic zone, ectopic presence of stromal progenitor markers in the renal medulla and
abnormal increase in the number of stromal cells, Ptc1-null kidneys also exhibited lower
glomerular number at E18.5. However, both qualitative and quantitative analyses of nephron
progenitors in the developing kidney did not show any marked decrease in this cell population,
suggesting that nephron progenitors survival and self-renewal is not affected by activated HH
signalling. This data implies that the number of stromal cells in our mutants is not increased at
the expense of a reduction in Six2+ nephron progenitors, and perturbation of HH signalling does
not change the fate of Osr1+Sall1+ cells already committed to nephrogenic fate. This is in
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agreement with previous published studies indicating the segregation of nephrogenic lineage,
marked by Eya1 expression, from the Osr1+ IM as early as E8.75 (Jinshu Xu et al. 2014).
Quantifying the number of nephron intermediate structures marked by NCAM revealed a
significant decrease in these structures. However, whether the decrease in NCAM+ structures
results from increased apoptosis of the developing nephrons or a defect in nephron
differentiation requires further investigation. Thus, it is necessary to examine the expression of
genes involved in nephron differentiation, such as wnt4,wnt9b and Lim1, and to determine the
apoptotic index within the nephron forming structures (Yu et al. 2009; Kobayashi et al. 2005).
Regardless of the mechanism underlying reduced nephron formation, it is very likely that the
changes in stromal compartment caused by activation of HH signalling affect the process of
nephrogengesis in a non- cell autonomous manner (Review of the signalling mechanisms in (Li
et al. 2014)).
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2.9 Conclusion
In recent years, there have been remarkable advances in our knowledge of the functional
role of stromal cells during kidney development. This has changed the historical view of the
stroma as merely a supportive cell compartment to recognize embryonic stromal cells as active
regulators of ureteric branching morphogenesis and nephron differentiation (Hatini et al. 1996;
Fetting et al. 2014; Bagherie-Lachidan, Reginensi, Pan, et al. 2015; Levinson et al. 2005). In the
adult kidney, the stromal lineage contributes to fibrosis in models of kidney injury, indicating the
critical role of stromal-derived cells beyond development(Humphreys et al. 2010; Gomez et al.
2014; Fabian et al. 2012). Despite the critical importance of stromal cells in normal renal
development, and the knowledge that Foxd1+ self-renewing stromal progenitors have the same
origin as nephron progenitors in Osr1+ posterior IM, there exists a lack of knowledge regarding
how the stromal lineage segregates from its common precursor population (Kobayashi et al.
2014; Mugford et al. 2008).
In this thesis, I present a novel stage-specific role for HH signalling in early renal
precursor cells expressing Sall1 and Osr1. Winny Li and Hovhannes Martyrosyan from the
Rosenblum lab have previously demonstrated the requirement for HH signalling in stromal cells
for proper renal capsule formation and regulation of nephrogenesis through a crosstalk with
nephron progenitors. Moreover, constitutive HH signalling activity in the metanephric field
resulted in an increase in stromal gene expression. The body of work presented here
demonstrates the effect of aberrant HH signalling activity in Sall1+ and Osr1+ renal precursors
between E9.5 and E10.5 in promoting stromal cell specification without affecting cell
proliferation/self-renewal in either the stromal or nephrogenic progenitor pools. I propose the
following model of stromal cell specification: an uncommitted pool of Sall+Osr1+ precursor
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cells exists in the posterior IM that either contributes to the stromal progenitor lineage or
undergoes apoptosis (or contributes to cell types other than nephrogenic or stromal). Activation
of HH signalling in this precursor population favors specification of more stromal cells. The
abnormally high number of stromal progenitors leads to disruption in their normal cortical
arrangement around the CM, and some of these cells migrate to the ectopic medullary location
close to the ureter. Yet, the body of work presented here does not fully define the molecular
mechanisms regulated by HH signalling that could potentially be involved in the specification of
stromal cells from their uncommitted precursor population.
Much information is yet to be revealed about the molecular pathways involved in
segregation of various renal lineages, particularly stromal progenitors from their common
precursors in the IM and un-induced MM. Considering the critical role of stromal cells in
regulating nephron differentiation and ureteric branching, understanding the pathways involved
in their initial specification have important implications for multiple renal disease processes and
for ongoing attempts at de novo kidney tissue regeneration, all of which have failed to generate
native stromal tissue thus far.
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2.10 Future Directions
To further verify the role of activated HH signalling in promoting stromal progenitor
specification from the Osr1+ precursor pool, it is necessary to complement our qRT-PCR
analysis on FACS-isolated Osr1+ cells by quantifying the number of stromal cells to that of
nephron progenitors through FACS analysis of control and HH-activated mutants. It was recently
shown by Ohomori et al. (2015) that stromal cells can be specifically isolated from nephron
progenitors through fractioning the FAC-sorted Osr1+ cells based on Pdgfrβ and Integrinα8
expression, where the Pdgfrβ + /Integrinα8− contains stromal cells, while Pdgfrβ − /Integrinα8+
population contains nephron progenitors (Ohmori et al. 2015; Taguchi et al. 2014).
To begin to elucidate the molecular mechanisms through which HH signalling activation
in the renal precursors enhances stromal lineage specification, it is necessary to perform a high-
throughput gene expression analysis on FACS-isolated Osr1+ cells. RNA sequencing can be
performed on total RNA extracted from Osr1+ cells sorted from caudal nephrogenic
mesenchyme of the Osr1EGFPCreERt2;Ptc1flox/- and heterozygous controls
(Osr1EGFPCreERt2;Ptc1flox/+) as early as E10.5, 24 hours after TM injection to the pregnant dam.
This will generate a validated set of differentially regulated genes that provides the basis for
functional analysis in controlling stromal cell specification and migration in vitro. A novel study
by Taguchi et al., 2014 established a protocol to derive MM from pluripotent stem cells (PSC) in
vitro, by using information about early renal development in vivo (Taguchi et al. 2014). These
progenitors were reported to reconstitute the 3D structures of the kidney in vitro, including
glomeruli with podocytes and renal tubules, but they completely lacked Foxd1+ stromal
compartment (Taguchi et al. 2014). Using data from the proposed RNA sequencing experiment,
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it is possible to generate novel protocols that direct cells toward both nephron and stromal
progenitor lineages.
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Chapter 3: Activated Hedgehog Signalling in Multipotent Renal Progenitors Leads to Prenatal
Hydronephrosis
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3.1 Introduction
Although congenital UPJ obstruction is a major cause of neonatal hydronephrosis, little is
known about in utero development of this disease. Previous work done in the Rosenblum lab has
demonstrated that increased HH signalling in the MM using a Rarb2Cre mouse model, leads to the
formation of a Ptc2+ ectopic cell mass at the junction of the ureter and pelvis as early as E16.5. UPJ
obstruction in these mice leads to antenatal hydronephrosis and death of the embryos following birth.
Remarkably, gene expression analysis on the isolated ectopic cell mass showed a significant increase
in cortical stromal markers in addition to downstream targets of HH signalling. Yet, the early cellular
origin of the ectopic cell mass, and the embryonic stage at which HH signaling activity in the cells of
origin leads to UPJ obstruction and hydronephrosis remained elusive.
Here, I addressed the temporal role of HH signalling specifically within Sall1+ and Osr1+
precursors of the kidney lineages in pathogenesis of UPJ obstruction. Activation of HH signaling in
these precursor population between E9.5-E10.5 leads to prenatal hydronephrosis caused by physical
obstruction of the UPJ by Raldh2+ cells. Analysis of Ptc1 expression as a readout of HH signaling
activity demonstrated that the obstructing cell mass in these models are also HH responsive.
Moreover, analysis of biopsies from human neonates with UPJ obstruction indicated a remarkable
increase in Ptc2, and Foxd1 expression in a subset of patients. Although the true etiology of UPJ
obstruction is multifactorial, our findings suggest a stage-specific pathogenic role of HH signalling in
the early precursor cells of the kidney marked by Osr1 and Sall1 to cause UPJ obstruction by a
cortical stromal cell mass.
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3.2 Experimental Methods
3.2.1 Intrapelvic Dye Injections
Visualization of the ureteropelvic lumen was performed as previously described (Airik et
al. 2006). Briefly, bromo-phenol blue dye was injected into the renal pelvis of isolated urogenital
systems at E18.5 using a pulled-out Pasteur glass pipette.
3.2.3 Patients’ Specimens Handling
For the patient group, I used excisional UPJ biopsies from 11 patients with the mean age
of 7.8 months (2 months- 3 years) who underwent pyeloplasty at the Hospital for Sick Children,
Toronto due to congenital UPJ obstruction. No patients had a known urinary tract infection prior
to the surgery. Studies were approved by the Research Ethics Boards (REB), and clinical data
was stored according to REB-approved procedures.
Specimens removed from patients were kept in normal saline on ice prior to being
sectioned into two identical halves. Half of the tissue was preserved in RNAlater®(Sigma) for
subsequent RNA extraction, and the other half was fixed in 4%PFA for histological examination.
The samples were then processed for paraffin-embedded sectioning and H&E staining as
discussed in the previous chapter. RNA extraction from the samples stored in RNAlater® was
carried out by combining TRIzol® RNA extraction protocol with QIAGEN RNeasy® micro kit
for RNA purification. Detailed description of cDNA synthesis and qRT-PCR methods can be
found in the previous chapter, while the primer sequences are listed in table 3.
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Table 3. Primer sequences used for qRT-PCR (Homo Sapiens)
3.2.4 Chromogenic Immunohistochemistry (IHC)
Immunohistochemical staining of human specimens were performed according to
published protocols (Orlando et al. 2013). Briefly, after tissue rehydration, antigen retrieval and
protein blocking steps, slides were incubated with primary antibodies, including rabbit
polyclonal anti-human PTCH2 antibody (LS-B301) or goat polyclonal anti human FOXD1
antibody (sc-47585). Endogenous peroxidase activity was blocked by incubating in hydrogen
peroxide for 15 minutes. For horseradish peroxidase (HRP) detection, sections were incubated
with appropriate biotinylated- secondary antibody (Vector) for 1 hour at room temperature. Next,
in a separate reaction, avidin and biotinylated peroxidase (Vector ABC Kit) were mixed
according to manufacturer instructions such that some of the binding sites on avidin were left
unoccupied. This complex was then incubated with the tissue sections. The unoccupied biotin-
binding sites on the complex bind to the biotinylated secondary antibody, amplifying the signal.
The brown product of the enzyme was developed using 3,3'- Diaminobenzidine (DAB) substrate
(Vector). Cell nuclei were counterstained with hematoxylin.
3.2.5 Quantification of IHC Staining
Intensity of the DAB staining, was quantitated and analyzed using ImageJ® software
following the instructions below (Jensen 2013; Safadi et al. 2010):
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In each image, the FOXD1 positive area (brown stain) and total area in the analyzed field
were automatically measured using color deconvolution plugin (Ruifrok AC, 2001). In the
present study, color deconvolution was used to separate the DAB stain from hematoxylin which
represents the whole nucleated area. Each image was changed into an 8-bit type (gray) and then
processed into binary (black and white) image. The measurement criteria was set to calculate the
area of the field (total nucleated area) and area fraction (area of black color representing DAB
stain). A score for FOXD1 expression in the analyzed field was calculated by dividing the
positively stained area over the total tissue area. For each immunostained UPJ sample, 5-6
different areas were analyzed independently to reach an average FOXD1 expression score.
3.2.6 Acknowledgement of Contributing Colleagues
The majority of the work discussed in this thesis was done by the author. However, I
would like to acknowledge technical assistance of Lijun Chi, who guided me through and
conducted the intrapelvic dye injection experiment (Figure 18K,L).
3.3 UPJ Obstruction and Hydronephrosis in Ptc1 Mutant Mice
When TM was administered to a Ptc1flox/flox dam bred with a Sall1CreERt2;Ptc1+/-male at
E10.5, no marked abnormalities were observed in kidneys at birth (Figure 18A,B). In contrast,
TM administration at E9.5 caused marked bilateral hydronephrosis and a thin renal cortex,
mimicking the phenotype observed in Rarb2-Cre; Ptc1-null mutants (Figure 18C,D). Moreover,
histological analysis revealed that the renal pelvis is not developed in Sall1CreERt2;Ptc1flox/-mice at
E16.5, whereas it is fully formed and connected to the ureter in the littermate controls
(Figure18E, F). To determine whether a physical obstruction of the urinary tract was associated
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with hydronephrosis in mutant mice, blue dye was injected into the renal pelvis of the dissected
urogenital region. In control Ptc1flox/+ kidneys (n = 9), dye traversed the length of the ureter and
entered the bladder (Figure18K). However, in all the mutant kidneys (n = 4), dye accumulated in
the renal pelvis, failing to pass this structure (Figure 18L). Together, these data demonstrated
that deletion of Ptc1 in Sall1+ cells starting at E9.5 causes an obstruction at the junction of the
ureter and pelvis.
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Figure 18 Activation of HH signalling in Sall1+ cells at E9.5 leads to abnormal pelvis and UPJ development
A,B. H&E staining showing when TM is injected at E10.5, Sall1CreERt2;Ptc1flox/- kidneys look
comparable to WT controls E18.5. C, D. When TM is injected at E9.5 mutant kidneys exhibit a
dilated renal pelvis and thinning of the cortex. E,F. Incomplete formation of renal pelvis, and an
absent connection with the ureter in mutants at E16.5 (marked by stars). G,H. Whole mount
preparations of E18.5 kidneys demonstrates comparable morphology of the mutants and controls
when TM is injected at E10.5.I,K. When TM is injected at E9.5 there is a marked
hydronephrosis in mutants, characterized by the accumulation of urine in the pelvis. Intrapelvic
dye injection demonstrates a blockage of flow in E18.5 mutant kidneys at the junction of the
ureter and pelvis in mutant mice.
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Osr1+ cells are functionally upstream of Sall1+ cells in the IM and have a broader
differentiation capacity. When I examined renal phenotypes of Osr1EGFPCreERt2;Ptc1flox/-embryos
at E18.5 following TM injection at E9.5, a phenotype similar to Sall1CreERt2;Ptc1-null model,
with marked hydronephrosis and a thin renal cortex was observed (Figure19A-D). Strikingly, in
Osr1EGFPCreERt2; Ptc1flox/- mice, hydronephroses had a unilateral occurrence, with the abnormal
kidney on the left side (n=5). This is consistent with the left-sidedness of the UPJ obstruction
occurrences in the majority of the patients diagnosed with congenital hydronephrosis (Zhang et
al. 2000). Immunostaining for Raldh2, a marker of cortical stroma, demonstrated ectopic
presence of Raldh2+ cells at the obstructed UPJ of the hydronephrotic mutants. In contrast,
Raldh2 expression was restricted to the renal cortex in the control kidneys (Figure 20E, F). This
data agrees with the expanded Raldh2 expression domain to the medullary stroma and ectopic
localization of Raldh2+ cells in the ureteral lumen observed at E15.5 (Figure 14). These results
confirm that increased HH signalling specifically during lineage segregation of stromal
progenitors leads to congenital UPJ obstruction associated with other renal abnormalities
(discussed before). Although the mechanisms underlying migration of the expanded and
disorganized stromal progenitors (shown in Figure 10) to the UPJ area remains to be elucidated,
it is likely that continuous activity of HH signalling plays an important role in this process.
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Figure 19 Activated HH signalling in Osr1+ cells at E9.5 leads to unilateral hydronephrosis
A,B. Whole mount preparations of E18.5 kidneys demonstrates unilateral(left-sided)
hydronephrosis in Osr1 EGFPCreERt2;Ptc1flox/- mutants. C,D. Mutant kidneys display a dilated renal
pelvis and thinning of the renal cortex at E18.5. E,F. Higher magnification of the ureteropelvic
junction (UPJ) area immunostained for Raldh2 (red), a cortical stromal marker, and DAPI (blue),
marking nuclei. There is a disconnection between the ureter (u) and pelvis in the mutants, with
ectopic expression of Raldh2 at the UPJ (arrow).
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3.4 HH Signalling is Active in UPJ Obstruction in Mutant Mice
Ptc1 is directly downstream of HH signalling, and is reported as a robust readout of HH
transcriptional activity (Pearse et al. 2001). To further explore HH signalling activity in UPJ
development, I examined Ptc1 expression in Osr1CreERt2;Ptc1 mutant kidneys using RNA in situ
hybridization at E18.5, when hydronephrosis and UPJ obstruction is observed. Ptc1 in control
kidneys is largely expressed in the medullary stroma surrounding the pelvis, and in the
developing nephrons (Figure 20C)(Yu et al. 2002). In mutant mice, Ptc1 expression was
increased in the expression domain observed in control mice, but was also expanded to the
nephrogenic zone, confirming the activation of HH signaling in Osr1+ cells descendants (Figure
20D). Closer examination of Ptc1 expression in mutants, revealed abnormal presence of Ptc1-
expressing cells at the UPJ area in the tissue between the ureter and pelvis (Figure 20E, F). This
suggests that the obstructing cells in UPJ are in activated state of HH signalling. Analysis of
other genes downstream of HH signalling such as Gli1, and Ptc2, the Ptc1 homolog exclusively
expressed in the obstructed UPJ in Rarb2Cre;Ptc1-null mice, will further validate this finding.
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Figure 20 Ectopic Ptc1 expression in the obstructed UPJ
In situ hybridization of Ptc1 RNA probe on E17.5 control and mutants with Ptc1 mutation targeted to
their Osr1+ cells at E9.5. A, B. Ptc1 is expressed in the inner and outer medullary stroma, and in the
glomeruli of the control kidney. Ptc1 is upregulated in the medullary stroma and is highly expressed in
the cortex of the mutant kidney. The circled area shows open UPJ in the control, and the obstructed UPJ
in the mutant. C, D. Higher magnification of the cortex, showing no Ptc1 expression in the nephrogenic
zone (arrowheads) of the control kidney. Ptc1 is highly expressed in the nephrogenic zone (arrowheads)
of the mutant. E, F. Higher magnification of the ureter showing Ptc1 expression in the cell layer adjacent
to the urothelium in both controls and mutants. There are Ptc1 expressing cells in the ureteric lumen at the
UPJ in the mutant (black arrows)
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3.5 Stromal Progenitors and Hedgehog Signalling Contribute To UPJ
Obstruction in a Subset of Human Neonates
Congenital UPJ obstruction is the most common cause of neonatal obstructive
hydronephrosis, with the prevalence of 1 in 1000 to 2000 neonates (Klein et al. 2011). However,
the molecular pathogenesis of this disease is poorly understood. Unpublished data from Lijun
Chi demonstrated higher mRNA expression of Ptc1, and Ptc2, two distinct readouts of HH
signalling, in addition to Foxd1, a stromal progenitor marker, in the UPJ obstructing cell mass
isolated from Rarb2-Cre;Ptc1flox/- kidneys. This, together with the findings discussed in the
preceding sections, raises the possibility that aberrant HH signalling activity may similarly lead
to congenital UPJ obstruction in human neonates through a defect in migration/differentiation of
cells of cortical stromal origin. To examine this hypothesis I first asked whether genes
differentially expressed in the obstructed UPJ in our mouse models are also mis-regulated in
human neonates diagnosed with congenital UPJ obstruction.
Due to extensive genetic heterogeneity and variable expressivity among individuals with
respect to clinical features, and perhaps also with age, I compared the expression of stromal
progenitor markers in UPJ tissue from each patient to that of the adjacent normal ureter from the
same patient by qRT-PCR (Vivante et al. 2015). Gene expression analysis demonstrated between
a 2.2-fold and a 12-fold increase in Foxd1 mRNA in the obstructed UPJ tissue, compared to the
adjacent control tissue, in patients 3, 5, 7, 10 and 11 (Figure 21A). Tbx18 is a marker of the
ureteral mesenchyme, and is essential for ureteral smooth muscle cell development (Airik et al.
2006). Moreover, lineage tracing studies has previously shown the contribution of Tbx18+ cells
to a subset of Foxd1+ stromal progenitors in mice (Bohnenpoll et al. 2013a). Our qRT-PCR
analysis did not show a marked change in Tbx18 expression in UPJO tissue compared to the
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ureter in 4 out of 5 patients with increased Foxd1, suggesting that ureteral mesenchymal cells
most likely do not contribute to the UPJ obstruction in these patients (Figure 21A). I further
validated our qRT-PCR results in patients 3 and 7, with the biggest change in Foxd1 expression,
by performing IHC on paraffin-embedded UPJ and their adjacent ureteric tissues (Figure 21 D-
H). Moreover, our IHC results demonstrated a significant increase in FOXD1 in patient 9, for
whom I did not obtain enough RNA to perform qPCR analysis (Figure 21H). On the contrary,
quantitative analysis of our IHC results did not show a significant change in FOXD1 in patients 5
and 7, possibly due to the low sensitivity of IHC assay to quantitatively detect smaller changes.
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Figure 21 Expression of stromal progenitors markers in the obstructed UPI compared to the adjacent normal ureter in human neonates.
A. Foxd1 mRNA is increased in the obstructed UPJ compared to the normal ureter in patient number 3, 5, 7, 10 and and 11 (Individual patients labelled U1-U10). B,C. H&E staining of a representative midsagittal section of a UPJ specimen and its adjacent ureteric tissue. D-G. Immunohistochemical staining using anti-FOXD1 antibody. U2 is a representative sample in which there is no increase in FOXD1 (D,E) ,whereas U3 demonstrates increased FOXD1 expression(F,G). Yellow arrowheads point to the FOXD1 expressing nuclei stained with both DAB and haematoxylin. Orange arrowheads point to the nuclei not expressing FOXD1. H. Semi-quantitative analysis of immunohistochemical staining for patients with increased Foxd1 mRNA. There is a significant increase in DAB intensity in U3, U7 and U9, for which I did not have enough RNA to perform qPCR. No significant change was detected in U5 and U7.
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As discussed above, the obstructing cells in Rarb2Cre;Ptc1flox/- mutants exhibited higher
Ptc1 and Ptc2 mRNA expression in compared to the non-obstructing cells, in response to HH
signaling activity. When I examined expression of Ptc1 and Ptc2 using qRT-PCR analysis, the
obstructed UPJ in patients 3, 5 and 10 were found to show a 5.6-, 2- and 3- fold increase in Ptc2
expression respectively (Figure 22). Interestingly, Ptc1 was not detected in patient 4 with highest
change magnitude in Ptc2 expression, whereas it was up-regulated in patient 6 with no change in
Ptc2 expression. Thus, it is likely that Ptc1 and Ptc2 expression provide distinct readouts of HH
signalling depending on particular genetic makeup of the patient. I further assessed
transcriptional activity in response to HH signalling by analyzing two other HH target genes,
Gli1 and Hhip (Holtz, K. a Peterson, et al. 2013). Based on our qRT-PCR analyses, Gli1 and/or
Hhip were increased by different magnitudes in patients with increased Ptc2 or Ptc1 (Figure 22).
Taken together, our data demonstrate that the obstructed UPJ in patients 3, 5 and 10
exhibit an increase in both Ptc2 and Foxd1 expression. Notably, although there was no increase
in Foxd1 or Ptc2 expression in patient number 6, Ptc1 expression was dramatically increased.
Overall, although there is a high variation in gene expression among different patients, our
findings indicate a preliminary correlation between activated HH signalling or Foxd1 expression
with UPJ obstruction, at least at the transcription level, in a subset of the patients analyzed here.
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Figure 22 Expression of HH signalling response genes in the obstructed UPI compared to the normal adjacent ureter in human neonates.
A. The graph shows an increase in Ptc2 expression in the obstructed UPJ compared to the normal ureter in patient number 3, 5 and 10 (Individual patients labelled U1-U10). In patient number 6, there is an increase in Ptc1 expression, but no change in Ptc2. B-E. Immunohistochemical analysis of PTCH2 expression in the obstructed UPJ. Yellow arrowheads point to the Ptc2-expressing cells stained with DAB in brown in conjunction with haematoxylin in purple. U6 represents a sample with no change in PTCH2 (B,C), whereas U10 demonstrates an increase in PTCH2 protein (D,E).
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3.6 Discussion
HH signalling pathway is crucial for development, and regulates cell fate determination,
proliferation and tissue patterning(Goodrich et al. 1997; Litingtung et al. 2000; Litingtung et al.
2002). HH signalling is particularly important in the urogenital system development as Shh
deficiency in the ureteric bud lineage, and truncating mutations in Gli3, are shown to cause renal
apalasia and hypoplasia (Hu et al. 2004; Grotewold et al. 2002; Blake et al. 2015). Moreover, it
was shown that aberrant activation of HH signalling, through targeting Ptc1 deficiency to the
ureteric epithelium, causes renal hypoplasia due to abnormal branching morphogenesis (Cain et
al. 2009). Work done by Lijun Chi from our lab demonstrated the pathogenic role of
constitutively active HH signalling in the MM induced by Rarb2Cre, resulting in prenatal UPJ
obstruction and hydronephrosis. However, the Rarb2 promoter is active in the nephrogenic
mesenchyme beginning at least from E9.5, and maintained in the MM-derived cells, excluding
the medullary stroma. Therefore, the spatial and temporal effect of Ptc1 deficiency cannot be
delineated using this model (Kobayashi et al. 2005). Here, I reported that that activation of HH
signalling in the renal precursor population marked by either Sall1 or Osr1 at E9.5 leads to the
same phenotype as Rarb2-Cre;Ptc1-null mutants. Yet, conditional activation of the pathway at
E10.5 does not lead to the same phenotype. Also, Ptc1 deletion in MM after the onset of ureteric
branching using Tcf21Cre/Pod1Cre has been previously shown to only cause mild cystic kidneys
and no prenatal hydronephrosis (Maezawa et al. 2012). Together, these data suggest that
abnormal activation of HH signalling prior to fate restriction in the renal precursors is a
contributing factor to pathogenesis of UPJ obstruction.
I showed the presence of Ptc1+Raldh2+ cells in the obstructing UPJ tissue in the mutants.
This coupled with the findings discussed in the previous chapter indicating the effect of activated
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HH signalling in promoting stromal lineage specification, further explains the contribution of
renal stromal progenitors to the pathogenesis of UPJ obstruction. There are currently two major
models of obstructive hydronephrosis that are both associated with developmental defects in
urothelial and smooth muscle differentiation (Fogelgren et al. 2015; Yan et al. 2014). Yet,
Marian Staite previously demonstrated no defect in the smooth muscle cells (marked by SMA)
and ureteric epithelium (marked by UPKIII) in Rarb2-Cre;Ptc1-null hydronephrotic kidneys,
which is consistent with the absence of hydroureter in these mutants. Moreover, since there is no
evidence in favor of contribution of Foxd1+ cells to ureteric smooth muscle cells, it is unlikely
that smooth muscle cells are affected in our models of UPJ obstruction (Kobayashi et al. 2014).
Thus, in cases where there are no defects in ureteric epithelium or smooth muscle cells, I propose
that an abnormally expanded stromal progenitor population (caused by aberrant activation of HH
signalling in their precursor compartment) could explain the pathogenesis of UPJ obstruction and
hydronephrosis.
Evidently, the true etiology of this complicated congenital abnormality in humans is
multifactorial and therefore has remained largely unknown. Here, I examined our proposed
model in 11 neonates who underwent pyeloplasty for constricted UPJ removal. I compared the
expression of Ptc1, Ptc2 and other HH signalling response genes as well as Foxd1, a marker of
stromal progenitors, through IHC and qRT-PCR. Our results demonstrated increased expression
of Ptc2 and Foxd1 in 3 patients at least at the RNA level. Although, I were not able to
quantitatively compare the expression of these genes at the protein level, our semi-quantitative
IHC measurements validates the increase in Foxd1 expression in least 2 of the patients identified
through qRT-PCR. Analyzing a larger patient sample population and using more sensitive
protein expression assays are necessary to confirm the contribution of migration/differentiation
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defects in stromal progenitors under abnormally activated HH signalling condition in human
neonates.
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3.7 Conclusion and Future Directions
Despite many rodent genetic models with adult-onset hydronephrosis, there are very few
models with prenatal development of congenital obstructive nephropathy, and among the latter
group conditional knockouts have mainly targeted the smooth muscle cells surrounding the
ureter or urothelium (Klein et al. 2011). Here, I present two UPJ obstruction models with over-
activation of HH signalling targeted to the renal precursors and their descendants within the
kidney proper. UPJ obstruction and severe hydronephrosis in these models are observed
secondary to the renal abnormalities discussed in the previous chapter. In addition, I identified
cells expressing markers of renal stromal progenitors in the obstructing UPJ, suggesting potential
migration/ differentiation defect in this lineage caused by over-activity of HH signalling in their
precursors. Future studies should focus on candidate molecular mechanisms involved in proper
migration of stromal progenitors and their cell-cell interactions with the surrounding
mesenchyme that might be regulated by HH signalling.
Interestingly, our mutants exhibited abnormal positioning of the gonads, suggesting
potential abnormalities in the entire urogenital system including the mesonephros (Figure 1).
Although the anatomy and gene expression patterns in the mesonephros are well characterized,
the mechanisms that regulate gonadal development, including the interactions of early
mesodermal tissues, are poorly understood (Murashima et al. 2014). It has recently been shown
that midline structures including the notochord and floor plate are the source of SHH essential
for mesonephros development (Murashima et al. 2014). Further investigation of our newly
identified mouse models in which the timing of HH signaling activation can be temporally
regulated would provide deeper insight into the role of HH signalling in mesonephros and
gonadal development.
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Building on the foundation of the work described in this thesis, future work should focus
on whole exome sequencing to discover mutations associated with abnormal HH signalling
activity or specification of renal stromal cells from the IM in patients with congenital UPJ
obstruction and hydronephrosis. This will provide further insights into the underlying causes of
human UPJ obstructions, characterization of the progression of the disease in utero, and perhaps
novel bio- markers for UPJO progression and severity.
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