contact: sabrina danilin [email protected] watch...s.danilin, g. bilger, h firat, e....

POSTER SESSION: Molecular Diagnostics, April 12-13, 2017 18340 Yorba Linda Blvd. Ste 107 PMB 427, Yorba Linda, CA 92886 LabRoots Corporation © 2017 T +1.714.463.4673 F +1.714.970.2173 [email protected] • labroots.com V a l i d a & o n o f o u r H T G E d g e S e q P l a 6 o r m f o r a c c u r a t e a n d s e n s i & v e b i o m a r k e r d i s c o v e r y S.Danilin, G. Bilger, H Firat, E. Schordan, Firalis SAS, Huningue, France F i r a l i s , l e a d i n g g r o u p i n B i o m a r k e r d i s c o v e r y a n d q u a l i fi c a & o n v a l i d a t e s i t s n e x t g e n e r a & o n g e n e e x p r e s s i o n H T G E d g e S e q p l a 6 o r m a t i t s s i t e i n H u n i n g u e , F r a n c e . V a s t fi e l d o f e x p e r & s e E x p e r & s e f r o m d i s c o v e r y t o m a r k e t S t a t e o f t h e a r t t o o l s S i m p l e w o r k fl o w S e n s i & v e c h e m i s t r y M i n i m a l s a m p l e i n p u t H T G E d g e S e q t e c h n o l o g y 8 patient samples were analyzed using both HTG WTA miRNA panel and qPCR Exiqon miRNA panel V4 (341 miRNA in common). Results show a good correlation between both methods. H T G e d g e s e q o ff e r s a c c u r a t e r e s u l t s H T G e d g e s e q o ff e r s h i g h r e p r o d u c i b i l i t y Comparison between 4 independant experiments (sets) on the same sample using the miRNA WTA assay Sets 1,2,3 : HTG (Tucson, USA) with different instruments/ days/operators Set 4 : Firalis, (Huningue, France) H T G m e a n b y m i R N A 0 2 4 6 8 1 0 1 2 q P C R m e a n b y m i R N A 4 0 3 5 3 0 2 5 2 0 Sample correla&on Set1/Set2 0.999 Set1/Set3 0.998 Set2/Set3 0.999 Set3/Set4 0.993 E x t r a c & o n f r e e Eliminates biases from RNA extracition S e n s i & v i t y o f n e x t g e n e r a & o n s e q u e n c i n g V a r i o u s p a n e l s a r e a v a i l a b l e W T A a s s a y : a s t u d y e x a m p l e Project outlines - Validation of miRNAs with predictive value previously identified. - Samples: 15 µl of plasma from 75 patients with negative outcome and 67 patients with positive outcome. - Screening of miRNAs 24 control with HTG EdgeSeq WTA Results: - 313 miRNAs are above the threshold in plasma samples. - Predictive value of selected miRNAs confirmed and new miRNAs identified. ! HTG Edgeseq platform available at Firalis offers a new cost effective and reliable tool for accurate and sensitive RNA expression measurement. ! Its low sample input requirements and extraction free process make it an invaluable tool for biomarker discovery. C o n t a c t : e r i c . s c h o r d a n @ fi r a l i s . c o m C o n c l u s i o n 2084 miRNAs (miR20 database) Oncology biomarkers Whole transcriptome miRNA assay 24 HK Immunoonco drug response and immuno response Lymphomaassociated genes assay WATCH: Poster Presentation https://youtu.be/eQBS0jGinwI CONTACT: Sabrina Danilin sabrina.danilin@firalis.com

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Page 1: CONTACT: Sabrina Danilin sabrina.danilin@firalis.com WATCH...S.Danilin, G. Bilger, H Firat, E. Schordan, Firalis SAS, Huningue, France Conclusion Firalis, elading rgoup ni Biomarker

POSTER SESSION: Molecular Diagnostics, April 12-13, 201718340 Yorba Linda Blvd. Ste 107PMB 427, Yorba Linda, CA 92886

T +1.714.463.4673F +1.714.970.2173

[email protected] • labroots.com

Valida&on of our HTG EdgeSeq Pla6orm for accurate and sensi&ve biomarker discovery

S.Danilin, G. Bilger, H Firat, E. Schordan, Firalis SAS, Huningue, France

Conclusion

Firalis, leading group in Biomarker discovery and qualifica&on validates its next genera&on gene expression HTG EdgeSeq pla6orm at its site in Huningue, France.

Vast field of exper&se Exper&se from discovery to market State of the art tools

Simple workflow

Sensi&ve chemistry

Minimal sample input HTG EdgeSeq technology

8 patient samples were analyzed using both HTG WTA miRNA panel and qPCR Exiqon miRNA panel V4 (341 miRNA in common).

Results show a good correlation between both methods.

HTG edgeseq offers accurate results

HTG edgeseq offers high reproducibility

Comparison between 4 independant experiments (sets) on the same sample using the miRNA WTA assay

Sets 1,2,3 : HTG (Tucson, USA) with different instruments/days/operators

Set 4 : Firalis, (Huningue, France)

HTG mean by miRNA 0 2 4 6 8 10 12

qPCR

mea

n by

miR

Sample correla&on

Set1/Set2 0.999

Set1/Set3 0.998

Set2/Set3 0.999

Set3/Set4 0.993

Extrac&on free Eliminates biases from RNA

extracition Sensi&vity of next genera&on sequencing

Various panels are available

WTA assay : a study example Project outlines - Validation of miRNAs with predictive value previously identified. - Samples: 15 µl of plasma from 75 patients with negative outcome and 67 patients with positive

outcome. - Screening of miRNAs 24 control with HTG EdgeSeq WTA

Results: - 313 miRNAs are above the threshold in plasma samples. - Predictive value of selected miRNAs confirmed and new miRNAs identified.

! HTG Edgeseq platform available at Firalis offers a new cost effective and reliable tool for accurate and sensitive RNA expression measurement.

! Its low sample input requirements and extraction free process make it an invaluable tool for biomarker discovery.

Contact : [email protected]

Conclusion

2084 miRNAs (miR20 database)

Oncology biomarkers

Whole transcriptome miRNA assay 24 HK

Immuno-‐onco drug response and immuno response

Lymphoma-‐associated genes assay

WATCH: Poster Presentation https://youtu.be/eQBS0jGinwI CONTACT: Sabrina Danilin [email protected]