color alert a carbon monoxide detecting paint nicole yu
TRANSCRIPT
CARBON MONOXIDE POISONING
leading cause of poisoning death odorless, tasteless, colorless =
extremely hard to detect high concentration are fatal within
minutes impair the blood’s ability to transport
oxygen suffocation
CURRENT TECHNOLOGY
carbon monoxide detectors are somewhat effective
must be installed at certain locations in order to remain accurate
cannot be exposed to sunlight, humidity, and other conditions
false positives
COLOR ALERT
Requires: E. Coli bacteria amplifier gate cutM-cutS-cutL gene cutM-cutS-cutL gfp reporter gene ampicillin resistance gene KODE technology paint
Plasmid inserted
into bacteria
Ampicillin
resistance gene
carbon monoxide activator
for amplifier
gate
cutM-cutS-cutL gene
cutM-cutS-
cutL gfp =reporter gene
HOW IT WORKS
plasmid inserted into E. Coli bacteria grown on Agar plates with ampicillin and Luria broth
eliminate any bacteria that has not taken in the plasmid CO will activate the amplifier gate which will activate the
transcription factor activate the open reading frame of cutM-cutS-cutL gene
produce carbon monoxide dehydrogenase = enzyme that catalyzes conversion from CO to CO2
CO + H2O --> CO2 + 2 H+ + 2e (Carbon Cycle)
activation of cutM-cutS-cutL -- cutM-cutS-cutL gfp GLOW GREEN!!
KODE TECHNOLOGY
contains amphipathic function-spacer-lipid constructs hydrophilic functional head hydrophilic spacer lipid tail
KODE TECHNOLOGY CONT. modify a surface through process known as koding lipid tail acts like an anchor
spontaneously integrates into the phospholipid bilayer of the cell membrane
functional head is interchangeable depending on what function something that can attach to material of the wall
being painted cells secured to the wall https://www.youtube.com/watch?t=53&v=U14EnuNyJZc
EXPECTED RESULTSCarbon
MonoxideCarbon
Monoxide Dehydrogenase
Green Flouresence
0
0 0
1
1 1
https://scratch.mit.edu/projects/71362336/#player
WHY COLOR ALERT?
can detect both the presence and origin of CO
beneficial to those with hearing impairments who cannot hear the traditional smoke alarms
simple incorporation into house = more accuracy, less mistakes from misplacement or sensitivity
FLAWS & RISKS OF THIS DESIGN uncertain how long bacteria can survive in
such environment system may become extremely impractical
if bacteria does not have a prolonged life span
how effective FSL constructs are over multiple generation
cannot be seen when asleep in laboratory, precautions must be taken to
prevent bacteria from escaping if bacteria gets into environment, could
evolve and horizontal gene transfer could occur with other bacteria
TESTING & POTENTIAL ensure the efficacy of the device indicate possible changes that could be
implemented to improve the system if effective, design could be applied to
other areas such as cars or mines could also lead to the development of
organisms to replace other alarm systems
SOURCES Anand, Ashima, T. Satyanarayana, and & Satyanarayana: Applicability Of Co Dehydrogenase. "Applicability
of Carboxydotrophic Bacterial Carbon Monoxide Dehydrogenase(CODH) in Carbon Sequestration and Bioenergy Generation." Journal of Scientific & Industrial Research (2012): 381-84. NISCAIR Online Periodicals Repository. 16 Apr. 2012niscair online periodicals repository. Web. 29 July 2015. <http://nopr.niscair.res.in/bitstream/123456789/14154/1/JSIR%2071%286%29%20381-384.pdf>.
"Biosurface Innovation Has Never Been Easier." KODE Biotech. KODE Biotech, 2012. Web. 29 July 2015. <http://www.kodebiotech.com/>.
"CO Dehydrogenase/Acetyl-CoA Synthase (CODH/ACS)." Chemwiki. UC Davis ChemWiki, n.d. Web. 29 July 2015. <http://chemwiki.ucdavis.edu/Wikitexts/UC_Davis/UCD_Chem_124A%3A_Berben/CO_dehydrogenase/CO_dehydrogenase_2>.
"Function-spacer-lipid Construct." Wikipedia. Wikimedia Foundation, 1 Jan. 2015. Web. 29 July 2015. <https://en.wikipedia.org/wiki/Function-spacer-lipid_construct>.
Kang, Beom S., and Young M. Kim. "Cloning and Molecular Characterization of the Genes for Carbon Monoxide Dehydrogenase and Localization of Molybdopterin, Flavin Adenine Dinucleotide, and Iron-Sulfur Centers in the Enzyme of Hydrogenophaga Pseudoflava." Journal of Bacteriology. American Society for Microbiology, Sept. 1999. Web. 29 July 2015. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94076/>.
"Kodecyte.com." Kodecyte.com. N.p., n.d. Web. 29 July 2015. <http://www.kodecyte.com/>. K.R. Tremblay, Jr. "Preventing Carbon Monoxide Problems." Preventing Carbon Monoxide Problems. Colorado
State University, 3 Mar. 2015. Web. 29 July 2015. <http://www.ext.colostate.edu/pubs/consumer/09939.html>.
"MSHA - Carbon Monoxide." United States Department of Labor. U.S Department of Labor, n.d. Web. 29 July 2015. <http://www.msha.gov/Illness_Prevention/healthtopics/carbonmonoxide.htm>.
IMAGE LINKS
http://www.fleetfarm.com/products/images/medium/0000000014635.jpg http://www.nfpa.org/~/media/images/research-and-reports/report-graphic
s/nonfirecarbonmonoxide.jpg?as=1&iar=1&la=en
http://brokelyn.com/wp-content/uploads/2012/09/carbon-monoxide-detector.gif
http://media.cleveland.com/insideout_impact/photo/first-alert-detectjpg-e6b0f063e76062e5.jpg
https://upload.wikimedia.org/wikipedia/commons/thumb/9/9f/Structural_analogy_of_a_sunflower_to_space_filling_models_of_selected_FSL_constructs.png/300px-Structural_analogy_of_a_sunflower_to_space_filling_models_of_selected_FSL_constructs.png
https://upload.wikimedia.org/wikipedia/commons/thumb/8/84/Koded_biomembrane.svg/2000px-Koded_biomembrane.svg.png
http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/Articleimage/2011/MT/c1mt00042j/c1mt00042j-f4.gif