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CHOICE BASED CREDIT SYSTEM (CBCS)

SCHEME OF STUDIES AND EXAMINATIONS

B.Sc. (HONS.) – M.Sc. DUAL DEGREE IN BIOTECHNOLOGY

(EFFECTIVE FROM SESSION 2017-18)

DEPARTMENT OF BIOTECHNOLOGY

DEENBANDHU CHHOTU RAM UNIVERSITY OF SCIENCE &

TECHNOLOGY

Approved in 12th meeting of Academic Council (1st Year and 13th meeting of Academic Council (2nd Year)

MURTHAL (SONEPAT) HARYANA – 131039


DEENBANDHU CHHOTU RAM UNIVERSITY OF SCIENCE & TECHNOLOGY, MURTHAL (SONEPAT)

SCHEME OF STUDIES & EXAMINATIONSDepartment of Biotechnology

B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology(Effective from Session 2017-18)

Semester-I

Category Course Code Course Title

Teaching

SchemeExamination Marks

Duration of

ExamCredits

L PMarks

of class work

External Marks Total

Core Course

DBT101 Basics of Cell Biology 4 - 25 75 100 3 4DBT103 Botany-I 4 - 25 75 100 3 4

Generic Elective

DCH109 Chemistry-I 4 - 25 75 100 3 4DMT103 Mathematics-I 4 - 25 75 100 3 4

Core Lab DBT105 Cell Biology Lab - 4 20 30 50 3 2Generic Elective Lab

DCH 111 Chemistry Lab-I - 2 20 30 50 2 1

Ability Enhancement

DEN101 English-I 2 - 20 30 50 3 2

GES201B *Environmental Studies 3 - -- 75 75 3 -

GES203B *Environmental Studies Field Work - - 25 -- 25 - -

TOTAL 21 0 185 465 2550 25

* The Environmental Studies (GES201B) and Environmental Studies Field Work (GES203B) are compulsory but qualifying courses only.

DEENBANDHU CHHOTU RAM UNIVERSITY OF SCIENCE & TECHNOLOGY, MURTHAL (SONEPAT)SCHEME OF STUDIES & EXAMINATIONS

Department of BiotechnologyB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

(Effective from Session 2017-18)Semester-II

Category Course Course Title Teachi Examination Marks Dura- Credits


Code

ng Schem

e tion of exam

L PMarks

of class work

External

MarksTotal

Core Course DBT102 Basics of Biotechnology 4 - 25 75 100 3 4

DBT104 Zoology-I 4 - 25 75 100 3 4Generic Elective

DCH112 Chemisty-II 4 - 25 75 100 3 4DMT 104 Mathematics-II 4 - 25 75 100 3 4

Core Lab DBT106Laboratory Techniques in Biotechnology

- 4 20 30 50 3 2

Generic Elective Lab DCH114 Chemistry Lab-II - 2 20 30 50 2 1

Ability Enhancement

DEN102 English –II 2 - 20 30 50 3 2

DBT108Statistics & Computing Skills in Biotechnology

2 - 20 30 50 3 2

TOTAL 20 6 4 420 180 23



(Effective from Session 2017-18)Semester-III



Teaching

Scheme

Examination Marks Dura-tion of exam

Credits

L PMarks

of class work

External

MarksTota

l

Core Course

DBT201 Introduction to Biochemistry 4 - 25 75 100 3 4

DBT203 Introduction to Microbiology 4 - 25 75 100 3 4

DBT205 Environmental Biotechnology 4 - 25 75 100 3 4

DBT207 Botany- II 4 - 25 75 100 3 4Generic Elective DCH215 Chemisty-III 3 - 25 75 100 3 3

Core Lab DBT209 Biochemistry and Microbiology Lab - 4 20 30 50 3 2

Generic Elective Lab DCH217 Chemistry Lab-III - 3 20 30 50 3 1.5Ability Enhancement

DBT211 Seminar-I - 2 50 - 50 - 1

TOTAL 19 9 215 435 650 23.5



(Effective from session 2017-18)


Semester-IV


Teaching

SchemeExamination Marks Dura-

tion of exam

CreditsL P

Marks of class

work

External

MarksTotal

Core Course

DBT202Introduction to Genetics & Molecular Biology

4 - 25 75 100 3 4

DBT 204 Introduction to Immunology 4 - 25 75 100 3 4

DBT206 Instrumentation in Biotechnology 4 - 25 75 100 3 4

DBT208 Zoology II 4 - 25 75 100 3 4Generic Elective DCH214 Chemistry-IV 3 - 25 75 100 3 3

Core Lab DBT210Immunology & Molecular Biology Laboratory

- 4 20 30 50 3 2

Generic Elective Lab

DCH216 Chemistry Lab-IV - 3 20 30 50 3 1.5

Ability Enhancement

DBT212 Seminar-II - 2 50 - 50 - 1

TOTAL 19 9 21519 435 215 23.5


Department of Biotechnology


B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology (Effective from session 2017-18)

Semester-V


Teaching


tion of exam

CreditsL P

Marks of class

workExtern

al Total

Core CourseDBT301Plant Biotechnology-I 4 - 25 75 100 3 4

DBT303Animal Biotechnology-I 4 - 25 75 100 3 4

* Discipline Specific Elective

DBT Elective-I 4 - 25 75 100 3 4

DBT Elective-II 4 - 25 75 100 3 4

Core LabDBT305Plant Biotechnology-

Lab - 4 20 30 50 3 2

DBT307Animal Biotechnology Lab - 4 20 30 50 3 2

**Inter Disciplinary Elective

- - 3 - 25 75 100 3 3

Ability Enhancement

DBT309Self Study - - 50 - 50 3 1

TOTAL 19 8 3 360 215 4

*LIST OF DISCIPLINE SPECIFIC ELECTIVES-I: Elective-I Elective-II

DBT321 Diagnostic Techniques DBT327 IPR in BiotechnologyDBT323 Biopharmaceutical

TechnologyDBT329 Biofertilizers and

BiopesticidesDBT325 Introduction to

BioinformaticsDBT331 Biotechnology in Human

Welfare

**INTERDISCIPLINARY ELECTIVE: The students will opt for an interdisciplinary elective course offered by other departments in the ongoing semester.

LIST OF INTERDISCIPLINARY ELECTIVES: offered by Department of Biotechnology for students of other departments.

DBT351: Analytical TechniquesDBT353: Patenting in Research




(Effective from Session 2017-18)Semester-VI


Teaching


tion of exam

CreditsL P

Marks of class

workExtern

al Total

Core CourseDBT302 Industrial

Biotechnology 4 - 25 75 100 3 4

DBT304 Genetic Engineering-I 4 - 25 75 100 3 4

*Discipline Specific Elective

DBT Elective-III 4 - 25 75 100 3 4DBT Elective-IV 4 - 25 75 100 3 4

Core LabDBT306 Industrial

Biotechnology Lab - 4 20 30 50 3 2

DBT308 Genetic Engineering Lab-I - 4 20 30 50 3 2

**Inter Disciplinary Elective

- - 3 - 25 75 100 3 3

Ability Enhancement

DBT310 Literature Survey & Research Report - 4 50 - 50 - 2

TOTAL 19 16 2153 435 650 6505

*LIST OF DISCIPLINE SPECIFIC ELECTIVES:

Elective-III Elective-IVDBT322 Medical Microbiology DBT328 Developmental BiologyDBT324 AgricultureBiotechnology DBT330 Enzyme BiotechnologyDBT326 Downstream Processing DBT332 Virology

**INTERDISCIPLINARY ELECTIVE: The students will opt for an interdisciplinary elective course offered by other departments in the ongoing semester.

LIST OF INTERDISCIPLINARY ELECTIVES: offered by Department of Biotechnology for students of other departments.

DBT352: Principles of BionanotechnologyDBT354: Principles of Bioinformatics


DBT101 BASICS OF CELL BIOLOGY B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I

L P Credits Class Work : 25 Marks4 - 4 Examination : 75 Marks

Total : 100 MarksDuration of Examination

: 3 Hours

__________________________________________________________________________________________COURSE OBJECTIVES:

To understand the structures and purposes of basic components of prokaryotic and eukaryotic cells, especially macromolecules, membranes, and organelles.

To understand the fundamentals of cell organelles and their interactions.UNIT-I

Origin of Life through the cell theory, pre-cellular evolution, artificial creation of "cells", Theory of Chemical Evolution, Biochemical composition of cells (Protein, lipids, carbohydrates, nucleic acids), Cell shape & Morphology, Eukaryotic and Prokaryotic cells, cell types (Bacteria, Plant and Animal cells).

UNIT-IICell wall; Plasma membrane; Transport across membrane, Modification of plasma membrane and intracellular junctions; Cytoskeleton; Protoplasm; Mitochondria; Chloroplast; ER; Golgi complex; Lysosome, endosome and microbodies; Ribosome; Centriole; Nucleus.

UNIT-IIICell cycle and its regulation, Mitosis & Meiosis their mechanisms and differences, Different type of cells: Totipotent cell, Pluripotent cells, Stem cells, Blood cells. Cell locomotion(Amoeboid, Ciliary, & Flagellar).Cancerous cells: Differences between normal cells and cancerous cells.

UNIT-IVNature of genetic material, nucleic acids, Chromosomes: discovery, morphology, chemical composition, structural organization of chromatids, centromere, telomere, chromatin, nucleosome organization, eu- and heterochromatin, special chromosomes (polytene, lampbrush chromosomes), banding patterns in human chromosomes.

COURSE OUTCOMES: Students will be able to understand the concepts of cell biology including cell

cycle. Students will be able to differentiate between normal and cancerous cells.

TEXT / REFERENCE BOOKS: Cell Biology - S.C. Rastogi Cell and Molecular Biology - Lodish Biotechnical cell biology - Veer Bala Rastogi

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsory and comprising five short answer type questions of equal marks spread over the whole syllabus. The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.


DBT103 BOTANY-IB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I



: 3 Hours

COURSE OBJECTIVES: T o understand the taxonomic diversity and need for classification of plants. To develop scientific insights into the development of many plant products that

have shaped our society

UNIT- IAlgae: General characters, habit, habitat and distribution, classification and economic importance. Life histories of algae belonging to various classes:Chlorophyceae – Volvox,Phaeophyceae – Ectocarpus,Rhodophyceae-PolysiphoniaFungi: General characters, habit, habitat and distribution, classification and economic importance. Life histories of Fungi-Mastigomycontina- Phytophthora,Zygomycotina-Mucor,Basidomycotina-Agaricus.

UNIT- IILichens: General structure, habit, habitat and distribution, classification and economic importance. Plant diseases: Casual organism, symptoms and control of following plant diseases. Rust & Smut of Wheat, White rust of Crucifers, Late blight of Potato, Red rot of Sugarcane, Citrus Canker. Bryophytes: General characters, habit, habitat and distribution, classification & economic importance. Life histories of following: Marchantia andFunaria

UNIT- IIIPteridophytes: General characters of pteridophytes, habit, habitat and distribution, affinities with bryophytes & gymnosperms, classification, economic importance, study of life histories of fossil Pteridophytes – Rhynia andPterisGymnosperms: General characters, habit, habitat and distribution, classification, types of fossils, fossil gymnosperms- Williamsonia & Glossopteris. Gymnosperms: Study of life histories of CycasandPinus, economic importance of gymnosperms

UNIT- IVAngiosperms Origin and evolution, habit, habitat and distribution, Primitive and advanced characters of Angiosperms. Classification of angiosperms.Diagnostic features and brief description of flowering plants Ranunculaceae, Brassicaceae, Malvaceae, Rutaceae, Fabaceae, Apiaceae, Acanthaceae, Apocynaceae, Asclepiadaceae, Solanaceae, Lamiaceae, Chenopodiaceae, Euphorbiaceae, Asteraceae, Lilliaceae and Poaceae (Including economic importance).

COURSE OUTCOMES: Students will have a broad understanding of the economical uses of plants in mod-

ern society. Acquire an increased awareness of plants & plant products encountered in every-

day life.

TEXT / REFERENCE BOOKS: College Botany:Vol. 1, Vol. 2 and Vol.3: (Dec 2010) by Pandey B.P. (Author) and S.

Chand Publishing House, New Delhi. Bhojwani, S.S. and Bhatnagar, S.P. 2000. The Embryology of Angiosperms. 4th re-

vised and enlarge edition. Vikas Publishing House, Delhi.


http://www.amazon.in/s/ref=dp_byline_sr_book_1?ie=UTF8&field-author=Pandey+B.P.&search-alias=stripbooks


DCH 109 CHEMISTRY-IB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I



: 3 Hours

Course Objectives: The basic objective of this course is to provide a foundation of organic and inorganic chemistry for undergraduate students in Physics and Biotechnology. With the help of results from quantum mechanical computations, students will get the in-depth knowledge about the structure of atom. They would further learn the various aspects of the chemical bonding in ionic and covalent inorganic molecules. Students will be able to develop an understanding and appreciation of both structure and chemical transformations of organic molecules. Students will acquire basic concepts of electronic structure and be able to apply them to solve problems from various areas of organic chemistry, including stereochemistry, reactivity patterns and synthesis. Student will be proficient to drawing organic chemical structures from names (and vice-versa), naming structures including stereoisomers and geometric isomer, knowledge of the two models of bonding used in organic chemistry and understanding the basic synthesis of alkanes and their reactions.

UNIT-IAtomic Structure: Limitations of Bohr’s theory, dual behaviour of matter and radiation, de Broglie’s relation, Heisenberg Uncertainty principle. Hydrogen atom spectra.Time independent Schrodinger equation and meaning of various terms in it.Significance of ψ and ψ2, Schrödinger equation for hydrogen atom. Radial and angular parts of the hydogenicwavefunctions (atomic orbitals) and their variations for 1s, 2s, 2p, 3s, 3p and 3d orbitals (Only graphical representation). Radial and angular nodes and their significance.Radial distribution functions and the concept of the most probable distance with special reference to 1s and 2s atomic orbitals. Significance of quantum numbers, orbital angular momentum and quantum numbers ml and ms. Shapes of s, p and d atomic orbitals, nodal planes. Discovery of spin, spin quantum number (s) and magnetic spin quantum number (ms).Rules for filling electrons in various orbitals, Electronic configurations of the atoms.Stability of half-filled and completely filled orbitals, concept of exchange energy.Relative energies of atomic orbitals, Anomalous electronic configurations.

UNIT-IIChemical Bonding and Molecular Structure Ionic Bonding: General characteristics, lattice energy and solvation energy and their importance in the context of stability and solubility of ionic compounds. Born-Landé equation (derivation excluded), Born Haber cycle and its applications, polarizing power and polarizability. Fajan’s rules, ionic character in covalent compounds, bond moment, dipole moment and percentage ionic character. Covalent bonding: VB Approach: Shapes of some inorganic molecules and ions on the basis of VSEPR and hybridization with suitable examples of linear, trigonal planar, square planar, tetrahedral, trigonal bipyramidal and octahedral arrangements. MO Approach: Rules for the LCAO method, bonding and antibonding MOs and their characteristics for s-s, s-p and p-p combinations of atomic orbitals, nonbonding


combination of orbitals, MO treatment of homonuclear diatomic molecules of 1st and 2nd

periods (including idea of s-p mixing) and heteronuclear diatomic molecules (CO, NO and NO+). Comparison of VB and MO approaches.

UNIT-IIIStructure and bonding: Hybridization, bond lengths, bond angles, bond energy, Shape of molecules, localized and delocalized chemical bond, Van der Waals interactions, Electronic Displacements- Inductive, electromeric, Resonance and mesomeric effects, hyper conjugation, Hydrogen bonding. Reactive intermediates – carbocations, carbanions, free radicals, carbenes, arynes and nitrenes (with examples).Sterochemistry-I: Concept of isomerism, types of isomerism. Geometric isomerism – cis-trans, syn-anti and E/Z notation with C.I.P rules.

UNIT-IVStereochemistry-II: Optical isomerism – elements of symmetry, molecular chirality, enantiomers, stereogenic center, optical activity, properties of enantiomers, chiral and achiral molecules with two stereogeniccentres, diastereomers, threo and erythrodiastereomers, meso compounds, resolution of enantiomers, inversion, retention and racemization. Relative and absolute configuration, sequence rules, D & L and R & S systems of nomenclature.Conformational isomerism–conformational analysis of ethane and n-butane, conformational analysis of cyclohexane, axial and equatorial bonds, conformation of mono substituted and disubstituted cyclohexane derivative. Newman projection and Sawhorse formulae, Fischer and flying wedge formulae. Difference between Configuration and conformation

Books Suggested:1. Lee, J.D. Concise Inorganic Chemistry, Pearson Education 2010 2. Huheey, J.E., Keiter, E.A., Keiter, R. L., Medhi, O.K. Inorganic Chemistry, Principles

of Structure and Reactivity, Pearson Education 2006. 3. Douglas, B.E. and Mc Daniel, D.H., Concepts & Models of Inorganic Chemistry, Ox-

ford, 1970 4. Shriver, D.D. & P. Atkins, Inorganic Chemistry 2nd Ed., Oxford University Press,

1994.5. R. Sarkar, General & Inorganic ChemistryPart I, New Central Book Agency (P) Ltd.,

2012.6. Morrison R.T. and Boyd P.S., Organic Chemistry, 5th Edn., Pubs: Allyn and Bacon

Inc., Boston, 1992.7. Mukerji S. M., Singh S. P. and Kapoor R. P., Organic Chemistry Vol. I/II, Pubs:

Wiley Eastern Ltd., New Delhi, 1985.8. Loudon G. M., Organic Chemistry 4th edition, Oxford University Press.9. Wade L.G.Jr., Organic Chemistry, Pubs: Prentice-Hall,1990.10. Solomons G., Fundamentals of Organic Chemistry, Pubs: John Wiley,2002.11. Carey F.A., Organic Chemistry, Pubs: McGraw-Hill, Inc, 2003.12. Streitwisser A., Jr. and Heathcock C.H., Introduction to Organic Chemistry,

3rd Edn., Pubs: MacMillan Pub. Co., N.Y, 1992.

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsoryand comprising five short answer type questions of equal marks spread over the whole syllabus.The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.


DMT 103 MATHEMATICS-I B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I



: 3 Hours

Course Objectives: The objective of the course is to familiarize the students with basic Mathematics such as Trigonometric Functions, complex numbers, Matrices, Two & Three Dimensional Geometry.

Unit-IReview of trigonometric functions, sum and product formulae for trigonometric functions, Trigonometric Equations.

Unit-IIComplex Numbers and Quadratic Equations, Permutations and combinations, Binomial Theorem, sequences and series, Exponential and Logarithmic series.

Unit-IIIMatrices, Operations on Matrices, Determinants, singular and non-singular matrices, Adjoint and inverse of a matrix.

Unit-IVCo-ordinate Geometry: Rectangular Coordinate system, Straight lines, Circles and family of circles. Parabola, Ellipse and Hyperbola-their equations in standard form.

Three dimensional space, Coordinates of a point in three dimensional space. Distance between two points, Section Formula.

Suggested Books

Scope as in the relevant chapters of the books:1. Mathematics, A Text book for Class XI and XII, NCERT, New Delhi.2. Mathematics for class XI of Vol. I & II by R.D. Sharma.3. Mathematics Text Book for class XI by R.S. Aggarwal4. Comprehensive Mathematics for class XI volume I & II by Luxmi Publication5. Elements of Mathematics for class XI by M.L. Bhargava, Janardan Dinodia

Note: The question paper will have four parts and each part will have two questions. The candidate will be required to attempt five questions in all by selecting at least one question from each part.

DBT105 CELL BIOLOGY LABB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology


Semester-I

L P Credits Class Work : 20 Marks4 2 Examination : 30 Marks


: 3 Hours

COURSE OBJECTIVES: This course will describe different parts of microscope and cell staining techniques. To prepare and identify different types of slides.

LIST OF EXPERIMENTS 1. To study structure of Prokaryotic and Eukaryotic cells.2. To study ultra- structures of cell organelles.3. To isolate chloroplast from leaves.4. To isolate the mitochondria from given sample.5. To identify the blood cell types in human blood smear.6. To Stain different type of prokaryotic & Eukarytic cells.7. To perform Gram’s staining for bacteria.8. To identify the presence of Barr body in the Female buccal cavity.9. To study different stages of Mitosis in onion root tips.10. To study meiotic cell division in given sample (testis follicles)

COURSE OUTCOMES: Students will be familiarized with techniques used in cell identification,

cell culture and organelle staining.

TEXT / REFERENCE BOOKS: A laboratory handbook, 3rd edition, Julio E.Celis, 2006, Elsevier Academic Press, USA. Essential Cell Biology: A Practical Approach vol 1: Cell structure (Practical Ap-proach Series) Ist edition, John Davey, Oxford University Press, UK.

Note: The students will be required to perform 08 experiments/ exercises from the above list and the other two experiments may be designed by the department based on the related theory course.


DCH 111 CHEMISTRY LAB-IB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I

L P Credits Class Work : 20 Marks- 2 1 Examination : 30 Marks


: 3 Hours

Course Objectives: Objective of this course is to demonstrate safe laboratory practices through the use of appropriate personal protective equipment and proper handling of hazardous chemicals, including proper disposal of waste. Students will employ the major techniques used in chemistry laboratory for analyses such as melting point determination, boiling point determinations, viscosity, surface temsion and purification techniques. Students will demonstrate the ability to maintain a proper laboratory notebook, which includes clear descriptions of original data, observations and experimental procedures.

Experiments Preparation of solution: molar and normal Calibration and use of apparatus Preparation and standardization of solutions Determination of Melting points of unknown organic compounds Determination of Boiling Point of liquid compounds Purification of organic compounds by crystallization Determination of physical properties of pure liquids: Viscosity and surface tension

Note: The department can change any experiment depending on the availability of chemicals.

Books Suggested 1. Vogel’s Textbook of Quantitative Analysis, revised, J. Bassett, R. C. Denney, G.H.

Jeffery and J. Mendham, ELBS.2. Vogel’s Textbook of Macro and Semimicro Qualitative Inorganic Analysis, revised, G.

Svehla, Longman.3. Practical Inorganic Chemistry, Marr and Rocket. Applied Chemistry by O.P. Virmani

and A.K. Narula, New Age International.4. Organic Analytical Chemistry: Theory And Practiceby Jag Mohan, Narosa Publishing

House 5. Khosla, B. D.; Garg, V. C. & Gulati, A., Senior Practical Physical Chemistry, R. Chand & Co.: New Delhi (2011).6. Athawale, V. D. &Mathur, P. Experimental Physical Chemistry New Age International:

New Delhi (2001).



DEN 101 ENGLISH-I B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I



: 3 Hours

COURSE OBJECTIVES: To equip the students with the language skills needed in academic and professional world and inculcate in them the ability to critically read the literary texts so as to develop in them proficiency in reading along with a sensitivity towards the impact literary texts can have on their lives and also enhance their speaking and writing skills.

Unit-I: Reading Skills: Language through literature(i) “The Bet” by Anton Chekhov(ii) “Under the Banyan Tree’’ by R.K. Narayan(iii) “The Refugee” by Pearl S. Buck

Unit-II : Writing Skills: Basics of Grammar(i) Correct usage of Tenses(ii) Subject Verb Agreement

Unit-III: Speaking Skills: Pronunciation and Dialogues (i) IPA Symbols of ‘Received Pronunciation’: Identifying the symbols/sounds in the words

(ii) Phonetic Transcription of words using IPA symbols

Unit-IV: Written Communication and Composition(i) Paragraph Writing on current /social issues or given situations(ii) Letter Writing: Constituents of formal letter writing, writing formal letters in com-plete / semi blocked style; E-mail writing

Scheme of End Semester Examination (Major Test)1. The duration of examination will be 3 hours.2. Nine questions of six marks each will be set, out of which the students will have to attempt five questions.

3. First question of six marks will be compulsory. It will cover all the four units of the syllabus and will consist of either short answer questions or questions in ‘Do as Di-rected’ mode. Question No. 2 to 9 will be set from all the four units of the syllabus -- two from each unit of six marks each. Students will have to attempt four more ques-tions selecting one from each unit. 4. Recommended Pattern of questions in each unit will be as follows:

Unit-I: One question having subparts from the literary texts has to be answered in about 200 words each or may be set on vocabulary items from the texts themselves. The second question will be a comprehension passage from the texts or on explanation with reference to the context of the lines/ passage from the prescribed texts.

Unit-II: Two questions of six marks each will be set from this unit. They may be in the form of correcting the sentences / filling in the blanks / choosing the right alternative / matching the parts / completing the sentences or changing the form of verbs.

Unit-III: One question of six marks will be in the form of transcription of words into IPA symbols and the second question will be on recognizing the sound/ phoneme in underlined alphabet/s of given words.


Unit-IV: One question will be set on writing a paragraph of 200- 250 words on any one of the ideas / topics given. The second question will be set on writing a formal letter or an email.

TEXT/REFERENCE BOOKS:Bande, Usha and Krishan Gopal. Eds. The Pointed Vision. New Delhi: OUP, 2009. Bhatnagar, Nitin and Mamta Bhatnagar. Communicative English for Engineers and Profes-

sionals. New Delhi: Pearson Education, 2016.Konar, Nira. Communication Skills for Professionals. New Delhi: PHI Learning Pvt. Ltd.,

2009. Sinha, R.P. Current English Grammar and Usage with Composition. New Delhi: Oxford Uni-

versity Press, 2016. Hill, L.A. A Guide to Correct English. Oxford: OUP Bansal, R.K. and J.B. Harrison. Spoken English: A Manual of Speech and Phonetics. Orient

BlackSwan, 2013.Balasubramanian,T. A Textbook of English Phonetics for Indian Students. Macmillan,

2012.

NOTE: Evaluation for the internal marks will be as per the instructions given in the ordinance for the course.


GES201B ENVIRONMENTAL STUDIESB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IL P Credits Class Work : -3 - - Examination : 75 Marks


: 3 Hours

UNIT – IThe Multidisciplinary nature of environmental studies, Definition, scope and importance. Need for Public awareness

UNIT – IINATURAL RESOURCES:Renewable and non-renewable resources:Natural resources and associated problems.a) Forest resources: Use and over-exploitation: deforestation, case studies, Timber ex-ploitation, mining, dams and their effects and forests tribal people.b) Water resources: Use and over-utilization of surface and ground water, floods, drought, conflicts over water, dams-benefits and problems.c) Mineral resources: Use and exploitation, environmental effects of extracting and using mineral resources, case studies.d) Food resources: World food problems, changes, caused by agriculture and overgraz-ing, effects of modern agriculture, fertilizer-pesticide problems, water logging, salinity, case studies.e) Energy resources: Growing energy needs, renewable and non-renewable energy sources, use of alternate energy sources; case studies.f)Land resources: Land as a resource, land degradation, man induced landslides, soil ero-sion and desertification. Role of an individual in conservation of natural resources.Equi-table use of resources for sustainablelifestyles.

UNIT- IIIECOSYSTEMS:Concept of an ecosystem, Structure and function of an ecosystem, Producers, consumers and decomposers, Energy flow in the ecosystem, Ecological succession, Food chains, food webs and ecological pyramids.Introduction, types, characteristic features, structure and function of the following eco-system:a) Forest ecosystem, Grassland ecosystem, Desert ecosystem.b) Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries).

UNIT- IVBIODIVERSITY AND ITS CONSERVATIONS:Introduction – Definition: Genetic, species and ecosystem diversity.Biogeographically classification of India.Value of biodiversity: consumptive use, productive use, social, ethical, aesthetic and op-tion values.Biodiversity at global, National and local levels.India as a mega-diversity nation. Hot-spots of biodiversity.Threats to biodiversity: habitat loss, poaching of wildlife, man-wildlife conflicts.Endangered and endemic species of India.

UNIT – VENVIRONMENTAL POLLUTION:Definition, causes, effects and control, measures of:Air pollution, Water pollution, Soil pollution, Marine pollution, Noise pollution, Thermal Pollution, Nuclear hazards


Solid waste management: Causes effects and control measures of urban and industrial wastes.Role of an individual in prevention of pollution.Pollution case studies.Disaster management: Floods, earthquake, cyclone and landslides.

UNIT – VISOCIAL ISSUES AND THE ENVIRONMENT:a) From unsustainable to sustainable developmentb) Urban problems related to energyc) Water conservation, rain water harvesting, watershed managementd) Resettlement and rehabilitation of people; its problems and concerns, case studiese) Environmental ethics: Issues and possible solutionsf) Climate change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust, Case studiesg) Wasteland reclamation, Consumerism and waste productsh) Environment Protection Act, Air (Prevention and Control of Pollution) Act, Water (Pre-vention and Control of Pollution) Act, Wildlife Protection Act, Forest Conservation Acti) Issues involved in enforcement of environmental legislation, Public awareness

UNIT – VIIHuman population and the Environment., Population growth, variation among nations.Population explosion – Famility Welfare Programme, Environment and human health, Hu-man Rights, Value Education, HIV/ AIDS, Woman and Child Welfare.Role of Information Technology in Environment and human health. Case Studies.

TEXT/REFERENCE BOOKS:1. Agarwal, K.C. 2001, Environmental Biology, Nidi Pub. Ltd. Bikaner.2. Bharucha, Franch, The Biodiversity of India, Mapin Publishing Pvt. Ltd., India. 3. Brunner R.C. 1989, Hazardous Waste Incineration, Mc. Graw Hill Inc. 480p.4. Clark R.S., Marine Pllution, Slanderson Press Oxford (TB).5. Cunningham, W.P. Cooper, T.H. Gorhani, E & Hepworth, M.T. 2001, Environmental En-cyclopedia, Jaico Pub. House, Mumbai.1195p.6. De A.K., Environmenal Chemistry, Wiley Eastern Ltd.7. Down to Earth, Centre for Science and Environment ®.8. Gleick, H.P., 1993. Water in Crisis, Pacific Institute for Studies in Dev., Environment & Security, Stockholm Env. Institute, OxfordUniv., Press 473p.9. Hawkins R.E. Encyclopedia of Indian Natural History, Bomaby Natural History Scociety, Bombay. 10. Heywood, V.H. & Watson, R.T. 1995. Global Biodiversity Assessment.Cambridge Univ. Press 1140p.11. Jadhav, H & Bhosale, V.M. 1995, Environmental Protection and Laws, Himalaya Pub. House, Helhi 284p.12. Mckinney, M.L. & Schoch, RM 1996, Environmental Sciences Systems & Solutions, Web enhanced Edition 639p.13. Mhaskar A.K., Mater Hazardous, Tekchno-Sciences Publications (TB).14. Miller T.G. Jr. Environmental Science, Wadsoworth Publishing Co. (TB).15. Odum, E.P. 1971, Fundamentals of Ecology, W.B. Saunders Co. USA, 574p.16. Rao M.N. & Dutta, A.K. 1987, Waste Water Treatment. Oxford & IBH Publ. Co. Pvt. Ltd., 345p17. Sharma, B.K., 2001, Environmental Chemistry, Goel Publ. House, Meerut.18. Survey of the Environment, The Hindu (M).19. Townsend C., Harper J, and Michael Begon, Essentials of Ecology, Blackwell Sciences (TB).20. Trivedi, R.K., Handbook of Environmental Laws, Rules, Guidelines, Compliances and Standards, Vol. I and II Enviro Mdiea (R).21. Trividi R.K., Handbook of Environmental Laws, Rules, Guidelines, Compliances and Standards, Vol I and II Enviro Media (R).22. Trividi R.K. and P.K. Goel, Introduction to air pollution, Techno Sciences Pub. (TB).


23. Wagner K.D., 1998, Environmental Management, W.B. Saunders Co. Philadelophia, USA 499p.24. A text bok environmental education G.V.S. Publishers by Dr. J.P. Yadav.(M) Magazine (R) Reference (TB) TextbookNote: 1. Examiner will set eight questions. Students will be required to attempt five questions.2. The awards of this paper shall not be counted in the award of the Degree/DMC.

GES203B ENVIRONMENTAL STUDIES FIELD WORKB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-I

L P Credits Field work marks : 25- - - Examination : -


: -

FIELD WORK: Visit to a local area to document environmental assets – river/ forest/ grass-

land/ hill/ mountain. Visit to a local polluted site-Urban/ Rural/ Industrial/ Agricultural. Study of common plants, insects, birds. Study of simple ecosystems – pond, river, hill slopes, etc. (Field work equal to

5 lectures hours).

Note: The awards of this paper shall not be counted in the award of the Degree/DMC.


DBT102 BASICS OF BIOTECHNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 3 Hours

COURSE OBJECTIVES: To understand the fundamentals & principles of biotechnology. Students will be able to recognize the foundation of modern biotechnology.

Unit-IIntroduction to Biotechnology: What is Biotechnology? Historical perspectives, Modern and Old Biotechnology, Biotechnology an interdisciplinary pursuit. Branches of BiotechnologyScope and Future of Biotechnology.

Unit- IINucleic acids as genetic material, structure of A-, B- and Z-DNA, palindromic sequences, structure of RNA (t-RNA, m-RNA and r-RNA), DNA denaturation and renaturation. Overview of information flow in biology, central dogma.Basic concept of recombinant DNA technology, principles of gene cloning. Restriction modification systems, use of restriction enzymes in biotechnology, cloning vectors.

Unit -IIIIntroduction to Animal, Plant and Microbial Biotechnology; General characteristics of microorganisms, structure of bacteria and viruses, bacterial growth - bacterial growth curve, factors affecting bacterial growth; Plant tissue culture techniques, in vitro pollination and fertilization, embryo culture and its applications, embryogenesis and organogenesis, micropropagation, haploids and their applications, somaclonal variations and applications.Basic techniques in animal cell culture and organ culture.

Unit IVApplications of biotechnology in various fields: Agriculture (GM Foods, GM Crops), Environment (Waste water and sewage treatment), Medicines, Industry.

COURSE OUTCOMES: Student will understand the difference between old and modern biotechnology. Student will understand the principles that form the basis of DNA Technology.

TEXT / REFERENCE BOOKS: An Introduction to Biotechnology -Rup Lal A Textbook of Biotechnology - R C Dubey Cytology, Genetics and mol. Biology - P.K. Gupta Biotechnical cell biology - Veer Bala Rastogi


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DBT104 ZOOLOGY-IB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IIL P Credits Class Work : 25 Marks4 - 4 Examination : 75 Marks


: 3 Hours

COURSE OBJECTIVES: To understand the taxonomy, diversity, physiology and ecology of all major animal phyla. To understand the economical importance of chordates and non chordates.

Unit-IConcept of animal diversity and its significance: Introduction, Animal diversity (Genetic, Species and Ecological Diversity), Animal diversity at global and country level in brief; Protozoa : General characters, habit, habitat and distribution, Locomotion and osmoregulation, Economic importance; Porifera : General characters, habit, habitat and distribution, Coral and coral reefs, Economic importance; Coelenterata : General characters, habit, habitat and distribution, Polymorphism in hydrozoa, Economic importance.

Unit-IIHelminthes: General characters, habit, habitat and distribution, Economic importance; Annelida: General characters, habit, habitat and distribution, Economic importance; Arthropoda: General character, habit, habitat and distribution, Economic importance of silkworm, prawn and lac insect; Mollusca: General characters, habit, habitat and distribution, Economic importance, Pearl industry, shell industry, ornamental value of molluscs.

Unit-IIIEchinodermata: General characters, habit, habitat and distribution, Economic importance, Protochordata : General characters, habit, habitat and distribution of Hemichordates, Urochordates and Cephalochordates, Study of Foot in Mollusca, Affinities in Hemichordata, Amazing invertebrates- Offence and defence mechanism- Ink gland in Sepia, Bioluminescence- Firefly.

Unit-IV Economic Zoology: Economic Fishery : Food value of fishes, Fresh water Fish farming, Construction and Maintenance, Maintenance of glass aquarium and ornamental fishes; Sericulture: Types of Silk moth Morphology of mulberry silk moth, Life cycle, Rearing of silkworm, Economic importance; Apiculture: Types and caste, Honey comb , Bee keeping, Economic importance; Dairy Science: Economics importance-Milk and Milk Products, Poultry Science: Poultry breeds-Indigenous and exotic breeds, Feeding, Housing and Management, Food value-egg and meat, Poultry diseases-Small pox and Ranikhet; Goat Farming: Breeds, Feeding, Housing, Economic importance.

COURSE OUTCOMES:


Students will be able to identify and classify major groups of organisms.

TEXT/REFERENCE BOOKS:- Hyman, L.H.: The Invertebrates Vol. I & II ( McGraw Hill) Barnes, R.D: Invertebrate Zoology ( W.B.Saunders Co.) Jordan, E. L: Invertebrate Zoology ( S. Chand Co. New Delhi.)


DCH112 CHEMISTRY-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 3 Hours

Course Objectives: First two units of this course is designed to provide the fundamental concepts of physical chemistry, stressing thermodynamics and chemical equilibrium. Physical chemistry expands upon the elementary notions of interactions, equilibrium, bonding, heat, entropy and energy. This area of chemistry uses extensive mathematical tools (differential and integral calculus) to probe the intricate interactions involved in chemical systems. Thus, in order to succeed in this course, problem-solving skills must be practiced and developed.

Also in this course, students will learn the periodic variation of different physical and chemical properties of known elements. They will also understand the general chemistry of the compounds of different s- and p-block elements.

UNIT-IChemical Thermodynamics and Chemical Equilibrium: Objectives and limitations of Chemical Thermodynamics, State functions, thermodynamic equilibrium, work, heat, internal energy, enthalpy. First Law of Thermodynamics : First law of thermodynamics for open, closed and isolated systems. Reversible isothermal and adiabatic expansion/compression of an ideal gas.Irreversible isothermal and adiabatic expansion.Enthalpy change and its measurement, standard heats of formation and absolute enthalpies.Kirchoff’s equation. Second and Third Law: Various statements of the second law of thermodynamics. Efficiency of a cyclic process (Carnot’s cycle).Entropy. Entropy changes of an ideal gas with changes in P,V, and T. Free energy and work functions. Gibbs-Helmholtz Equation.Criteria of spontaneity in terms of changes in free energy. Third law of thermodynamics: Absolute entropies.

UNIT-IIChemical Equilibrium and Thermodynamics of Simple Mixtures: Partial molar quantities and their significance. Chemical potential and its variation with T and P. Fugacity function and its physical significance.Concept of activity and activity coefficient.General characteristics of chemical equilibrium, thermodynamic derivation of the law of chemical equilibrium, Van’t Hoff reaction isotherm. Relation between Kp, Kc and Kx. Temperature dependence of equilibrium constant-Van’t Hoff equation, homogeneous &heterogreneous equilibria, Le Chetalier’s principle.

UNIT-III


The Periodic Table and Chemical Periodicity: The relationship between chemical periodicity and electronic structure of the atom. The long form of the periodic table – Classification of elements in s, p, d and f block of elements. Periodicity in oxidation state of valence, metallic/non-metallic character, oxidizing or reducing behaviour; acidic and basic character of oxides; trends in bond type with position of element and with oxidation state for a given element; trends in the stability of compounds and regularities in methods used for extraction of elements from their compounds; Trends in the stability of coordination complexes. Anomalousbehaviour of elements of 2nd short period (Li to F) compared to other members in thesame groups of s & p block elements; The diagonal behaviour between elements, theinert pair effect; variability of oxidation states of transition elements, colour, magneticproperties and other characteristics of transition elements.

UNIT-IVThe s-block elements: Production and uses of metals; chemical reactivity and trends in alkali and alkaline earth metals; structure and properties of oxides, halides and hydroxides, coordination complexes, Organometallic compounds of alkali metals, Crown and Crypts, Behaviour of solutions in liquid ammonia.The p-Block Elements: Electronic configuration, atomic and ionic size, metallic/non-metallic character, melting point, ionization enthalpy, electron gain enthalpy, electronegativity, Allotropy of C, P, S; inert pair effect, diagonal relationship between B and Si and anomalous behaviour of first member of each group. Structure of oxides and oxiacids of N, P, S and Cl.

Suggested Books1. Mahan B.H., University Chemistry, Pubs: Norosa Publishing House, 1998.2. Puri B.R., Sharma L. R. and Pathania M. S., Principles of Physical Chemistry,

Pubs: Vishal Publishing Company, 2003.3. Sienko M.J. and Plane R.A., Chemistry principles and properties, Pubs: Mc

Graw-Hill, New York 1975.4. Lippincott W.T., Carett A.R. and F.H. Chemistry, A Study of Matter, Pubs:John-

Wiely, New York ,1977.5. Dickerson R.E., Gray H.B., Derensburg M.Y. and D.S. Darensbourg, Chemical

Principles, Pubs:Benjamin-Cummings Menlo Park ,1984.6. McQuarrie D.A. and Rock P., General Chemistry, Pubs:W.H. Freeman, New

York, 1984.7. Lee, J.D. Concise Inorganic Chemistry, Pearson Education 2010 8. Huheey, J.E., Keiter, E.A., Keiter, R. L., Medhi, O.K. Inorganic Chemistry, Princi-

ples of Structure and Reactivity, Pearson Education 2006. 9. Douglas, B.E. and Mc Daniel, D.H., Concepts & Models of Inorganic Chemistry,

Oxford, 1970 10. Shriver, D.D. & P. Atkins, Inorganic Chemistry 2nd Ed., Oxford University Press,

1994.11. R. Sarkar, General & Inorganic ChemistryPart I, New Central Book Agency (P)

Ltd., 2012.



DMT104 MATHEMATICS-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 3 Hours

Course Ojectives: In this course the students will learn about functions, limits, continuity, differentiability, integrability, differential equation which will be extremely useful to them.

Unit-IContinuity and Differentiability: Introduction, Limits, Continuity, Differentiability, Exponential and Logrithmic Differentiation, Derivative of a functions, Second order derivative. Mean Value Theorem.

Unit-IIApplication of derivative: Increasing and decreasing functions, Maxima and Minima, Rolle’s Theorem (without proof). Mean Value Theorem. Tangents and Normals. Indeterminate forms, L’Hospital’s Rule. Taylor and Maclaurin series (without proofs).

Unit-IIIIntegral Calculus: Integral as antiderivative. Integration by substitution, by partial fractions and by parts. Definite integral and its properties. Areas of bounded regions. The definition of integral of a real valued function of real variable as limit of sum motivated by the determination of area. Fundamental theorem of integral calculus.

Unit-IVDifferential Equations: Introduction & Basic concepts. General and particular solutions of a differential Equation. Formation of differential equation. Methods of solving First order and First degree Differential equations.

Suggested ReadingsScope as in the relevant chapters of the books:

1. Mathematics, A Text book for Class XI and XII (Parts I & II), NCERT, New Delhi.2. “Calculus” by Thomas & Finney, 9th Edition, Pearson Education. 3. Mathematics for class XII volume I & II by R.D. Sharma4. Comprehensive Mathematics for class XII volume I & II by Luxmi Publication5. Elements of Mathematics for class XII by M.L. Bhargava, Janardan Dinodia

Note: The question paper will have four parts and each part will have two questions. The candidate will be required to attempt five questions in all by selecting atleast one question from each part.


DBT106 LABORATORY TECHNIQUES IN BIOTECHNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 3 Hours

COURSE OBJECTIVES: Develop the basic laboratory techniques of a biotechnology lab. Develop critical thinking and practice accuracy in writing scientifically.

LIST OF EXPERIMENTS: 1. Safety measures in laboratories. 2. To learn Preparation and handling of solutions.3. To prepare the buffer at required pH. 4. To study different parts of Microscope.5. To study centrifugation for isolation of different fractions from cells/ tissues.6. To study the absorbance curve of a given sample.7. To study sterilization techniques.8. To prepare media for isolation of Bacteria and Fungi.9. To prepare media for invitro cultures of Plant cells and animal cells.10. To prepare dilutions for the isolation of microbes.

COURSE OUTCOMES: Developing multitasking skills by developing laboratory techniques. Create an accurate record of lab works and results of experiment.

TEXT / REFERENCE BOOKS: Lehninger: Principles of Biochemistry (2013) 6th ed., Nelson, D.L. and Cox, M.M.,

W.H. Freeman and Company (New York), ISBN:13:978-1-4641-0962-1 / ISBN:10:1-4641- 0962-1.

Introductory Practical Biochemistry (2014) .,S.K. Sawhney & R. Singh (Eds) Pub-lished by Narosa Publishing House,ISBN 10: 8173193029 / ISBN 13: 9788173193026.



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DCH 114 CHEMISTRY LAB-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 2 Hours

Course Objectives: This course would provide the students the practical knowledge about acid base titration, extra element detection and determination of physical properties of compounds.

Experiments Preparation of solutions: mole fraction Acid-Base Titrations

(i) Estimation of carbonate and hydroxide present together in mixture. (ii) Estimation of carbonate and bicarbonate present together in a mixture.

(iii) Estimation of free alkali present in different soaps/detergents To study the process of sublimation of organic compounds. Detection of extra elements using Lassaigne's extract (LE) Determination of physical properties of pure liquids: Refractive index and optical

activity


Books Suggested 1. Vogel’s Textbook of Quantitative Analysis, revised, J. Bassett, R. C. Denney, G.H.

Jeffery and J. Mendham, ELBS.2. Vogel’s Textbook of Macro and Semi micro Qualitative Inorganic Analysis, revised, G.

Svehla, Longman.3. Practical Inorganic Chemistry, Marr and Rocket. Applied Chemistry by O.P. Virmani and

A.K. Narula, New Age International.4. Organic Analytical Chemistry: Theory and Practiceby Jag Mohan, Narosa Publishing

House 5. Khosla, B. D.; Garg, V. C. & Gulati, A., Senior Practical Physical Chemistry, R. Chand & Co.: New Delhi (2011).6. Athawale, V. D. &Mathur, P. Experimental Physical Chemistry New Age International:

New Delhi (2001).



DEN 102 ENGLISH-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-II



: 3 Hours

COURSE OBJECTIVES:To enhance further writing and speaking skills of the students with an ability to critically read literary and other texts so as to improve their commutative and written proficiency along with reading competence through sensitive and analytical reading of the prescribed literary texts.

Unit-I: Reading Skills: Language through literature(i) “Benares” by Aldous Huxley(ii) “Experiment and Experience’’ by W.R. Niblett

Unit-II: Vocabulary Building (i) One Word Substitution(ii) Homonyms

Unit-III: Writing Skills: Basics of Grammar (i) Direct and Indirect Speech(ii) Correct usage of Conjunctions (Coordinating, Subordinating and Connecting Adverbs)

Unit-IV: Professional Communication(i) Report Writing :Format and Structure of report writing; Writing of reports(ii) Writing Notices/Circulars

Scheme of End Semester Examination (Major Test)1. The duration of examination will be 3 hours.2. Nine questions of six marks each will be set, out of which the students will have to attempt five questions. 3. First question of six marks will be compulsory. It will cover all the four units of the syllabus and will consist of either short answer questions or questions in ‘Do as Directed’ mode. Question No. 2 to 9 will be set from all the four units of the syl -labus -- two from each unit of six marks each. Students will attempt four more questions selecting one from each unit. 4. Recommended Pattern of questions in each unit will be as follows:Unit-I: One question having subparts from the literary texts has to be answered in about 200 words each or may be set on vocabulary items from the texts themselves. The second question will be a comprehension passage from the texts or on explanation with reference to the context of the lines/passage from the prescribed texts. Unit-II: Two questions of six marks each will be set from this unit. Questions may be in the form of filling in the blanks, using suitable words from those given in the brackets / supplying one word substitution for the phrases, expressions / matching the words and their meanings etc.Unit-III: Two questions of six marks each will be set from this unit. They may be in the form of correcting the sentences / filling in the blanks / choosing the right alternative / matching the parts / completing the sentences / changing the form of sentences /synthesis of sentences/framing sentences using connectives etc.


Unit-IV: One question will be set on writing a report on activities and issues related to campus/university life. The second question will be on writing a notice or a circular.NOTE: Evaluation for the internal marks will be based on the instructions as given in the Ordinance for the course.

TEXT/REFERENCE BOOKS: Roy, A. and P.L. Sharma. English for Students of Science. New Delhi: Orient

Longman, 1992. Bhatnagar, Nitin and Mamta Bhatnagar. Communicative English for Engi-

neers and Professionals. New Delhi: Pearson Education, 2016. Konar, Nira. Communication Skills for Professionals. New Delhi: PHI Learning

Pvt. Ltd., 2009. Sinha, R.P. Current English Grammar and Usage with Composition. New

Delhi: Oxford University Press, 2016. Hill, L.A. A Guide to Correct English. Oxford: OUP, 1968. Murali, Krishna C and Sunita Mishra.2nd edition. Communication Skills for En-

gineers, New Delhi: Pearson Education, 2016. Ashraf, Rizvi M..Effective Technical Communication. New Delhi: McGraw Hill Education Pvt. Ltd., 2014.


DBT 108 STATISTICS AND COMPUTING SKILLS IN BIOTECHNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IIL P Credits Class Work : 20 Marks2 - 2 Examination : 30 Marks


: 3 Hours

COURSE OBJECTIVES: The objective of the course is to give conceptual exposure of essential contents of

biocomputation to students. To familiarize the students with basic concepts in statistics.

Unit IStatistics: Definition, Importance & Limitation, Collection of data and formation of frequency distribution, Graphic presentation of Frequency distribution – Graphics, Bars, Histogram, Diagrammatic; Measures of Central Tendency – Mean, Median and Mode, Partition values – quartiles, deciles and percentiles; Measures of variation – Range, IQR, quartile, deciles and percentiles, quartile deviation and standard deviation and Lorenz Curve.

Unit IICorrelation Analysis: Correlation Coefficient; Assumptions of Correlation Analysis; Coefficients of Determination and Correlation; Measurement of Correlation- Karl Person’s Methods; Spearman’s Rank correlation; Concurrent Deviation the Correlation Coefficient; Real world Application using IT Tools.

Unit-IIIBasics of Computer and its EvolutionEvolution of Computer, Data, Instruction and Information, Characteristics of Computers, Various fields of application of Computers, Various Fields of Computer, Input-output Devices (Hardware, Software, Human ware and Firmware), Advantages and Limitations of Computer, Block Diagram of Computer, Function of Different Units of Computer, Classification of Computers. Different Number System (Decimal, Binary, Octal and hexadecimal) and their Inter Conversion

Unit – IVMemory & NetworkingPrimary Memory (ROM and it’s type – PROM, EPROM,EEPROM, RAM) Secondary memory- SASD, DASD Concept, Magnetic Disks – Floppy Disks, Hard Disks, Magnetic Tape, Optical Disks – CD ROM and it’s type (CD ROM, CD ROM-R, CD ROM-EO, DVD ROM, Flash Memory. Networking Concepts, Types of networking (LAN, MAN AND WAN), Analog and Digital Transmission.Different Topologies.

COURSE OUTCOMES: Students will understand the importance and value of statistical thinking, training,

and approach to problem solving, on a diverse variety of disciplines.

TEXT/REFERENCE BOOKS: The C Programming Language by Dennis M Ritchie, Brian W. Kernigham, 1988,

PHI. Information technology, Dennis P. Curtin, Kim Foley, Kunal Sen, Cathleen Morin,

1998, TMH An introduction to biostatistics, ed. Glover, T. and K. Mitchell, 2002, McGraw-Hill,

New York. Fundamentals of Biostatistics, ed. Bernard Rosner, 1999, Duxbury Press.

Fundamental of Biostatistics, ed. Veer Bala Rastogi, Rastogi Publication

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be


compulsory and comprising five short answer type questions of equal marks spread over the whole syllabus. The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.

DBBT201 INTRODUCTION TO BIOCHEMISTRY B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IIIL P Credits Class Work : 25 Marks4 - 4 Examination : 75 Marks


: 3 Hours

COURSE OBJECTIVES: T o explain various interactions involved in biological systems. To learn the structure, classification and chemical properties of carbohydrate pro-

teins, lipid, nucleic acids and porphyrins.UNIT-I

Introduction as a discipline-historical perspective, major landmarks in the development of biochemistry.Chemical Foundations of living systems: Molecular basis of life, Biological chemistry – Biomolecules, Interactions in biological systems: Intra and intermolecular forces, Electrostatic and hydrogen bonds, Disulfide bridges, Hydrophobic and hydrophilic molecules and forces, Water and weak interactions, Debye-Huckel Theory.

UNIT-IICarbohydrates: Classification, Monosaccharides – structures and function; reactions of monosaccharides- mutarotation, glycoside formation, reduction and oxidation, epimerization and esterification, polarimetry; important monosaccharides and disaccharide; Polysaccharides –overview, structure; important polysaccharide; plant polysaccharide; Glycosaminoglycans, Glycoproteins. Amino acids and Proteins: Amino acids as building blocks of proteins, their structure, classification and chemical properties; Forces stabilizing protein structure and shape. Different levels of structural organization of proteins; alpha- helix, beta pleated sheet, Ramachandran Plot.

UNIT-IIILipids: Fatty acids as building blocks of most lipids, their structure and properties, classification of lipids, General structure and function of major lipid subclasses: Acylglycerols, phosphoglycerides, sphingolipids, glycosphingolipids, terpenes, steroids, Prostaglandins

UNIT-IVNucleic Acids and Porphyrins: Structure and properties of nucleic acid bases, nucleosides and nucleotides, biologically important nucleotides, Physical and chemical properties of RNA/DNA. Hydrolysis of nucleic acids.Structure, properties and classification of porphyrins.

COURSE OUTCOMES: Understand the chemical constituents of cells and various types of weak interac-

tions between the biomolecules. Understand the synthesis of large biomolecules such as proteins, carbohydrates,

lipids, nucleic acids from simple precursors.

TEXT / REFERENCE BOOKS: Lehninger: Principles of Biochemistry (2013) 6th ed., Nelson, D.L. and Cox, M.M.,

W.H. Freeman and Company (New York), ISBN:13:978-1-4641-0962-1 / ISBN:10:1-4641- 0962-1. .


Biochemistry (2012) 7th ed., Berg, J.M., Tymoczko, J.L. and Stryer L., W.H. Free-man and Company (New York), ISBN:10:1-4292-2936-5, ISBN:13:978-1-4292-2936-4.

Principles of Biochemistry (2008) 3rd ed., Voet, D.J., Voet, J.G. and Pratt, C.W., John Wiley & Sons, Inc. (New York), ISBN:13: 978-0470-23396-2

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsory and comprising five short answer type questions of equal marks spread over the whole syllabus.The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.

DBT203 INTRODUCTION TO MICROBIOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IIIL P Credits Class Work : 25 Marks4 - 4 Examination : 75 Marks


: 3 Hours

COURSE OBJECTIVES: To introduce the microbial world to the students To investigate microbial origin, diversity & application of microbiology

UNIT-IIntroduction to the Microbial World: Discovery of microbial world, Controversy over origin of microorganisms- Spontaneous generation vs. Biogenesis, Germ theory of diseaseDevelopment of pure culture methods: Use of Petri-dish and agar, Isolation of pure cultures & its significance, Developments in microbiology before and after 20th century. Contributions of some eminent scientists

UNIT-IIMicrobial Evolution, systematics and taxonomy: Evolution of earth and earliest life forms, primitive organisms and their sequential evolution, Classification systems and their utility .Bionomial nomenclature, Whittakers five kingdom and Carl Woese’s three kingdom classification system, Criteria used for classification of organisms-phenotypic, Biochemical, ecological, cultural, immunological molecular etc. Common classification systems: Phenetic, Numerical Taxonomy, Phylogenetic.

UNIT- IIIMicrobial Diversity Bacteria: General Characteristics, Morphology & cell structure, concept of Eubacteria & Archaebacteria, classification of bacteria.Fungi : Introduction, habitat, cell structure, classification & economic importance of fungi.Viruses : General properties of viruses, viriods, virusoids, prions and satellite viruses. Structure, cultivation and taxonomy of viruses in brief. Algae & Protozoa: General characteristics, occurrence, morphology and classification.

UNIT-IVScope of Microbiology:Environmental microbiology: Microbes in air, water, soil, Biofertilizers, Biogeochemical cycling, Biodegradation, Biomagnification, Biofilms, Composting, Bioinsecticides, Biopesticides, Bioremediation, Sewage treatment etc.Food and Dairy Industrial Microbiology: Fermented food, alcoholic Beverages, Bread, cheese, other fermented milk products, SCP.Industrial: Fermentation, Alcoholic Beverages, Antibiotics, Amino acids, organic acids, biopolymers, microbial enzymes.Medical Microbiology: Important human disease and their microbial causative agents.COURSE OUTCOMES: Describe diversity of microorganisms Explain the basic genetic systems of bacteria, virus and fungi


TEXT / REFERENCE BOOKS: Microbiology,ed. Prescott etal. 2007,Mc Graw Hill, USA. Microbiology,ed. Pelczaretal.Tata Mc Graw Hill, New York. Principles of microbiology, edR.M . Atlas, Mac Millian Publishing Co. New York. Immunology, ed. Kubyetal W.H. Freeman &Company, New York.


DBT205 ENVIRONMENTAL BIOTECHNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-III



: 3 Hours

COURSE OBJECTIVES: Explain various interactions involved in environment. To study the role of microorganisms in nutrient cycling and biodegradation.

UNIT-IIntroduction: Basic components of Environment, historical aspects, climate change, energy from waste, , Environmental problems and Environmental monitoring through microorganism,Environment Microbiology: organisms in nature & their importance, sampling, culture & cultivation of microorganisms, microbes in service of nature and mankind, batch culture & continuous culture of microbes for commercial use, role of genetic engineering, development vectors for efficient degradation

UNIT-IIMicrobes and Environment: Role of microorganisms in natural system and artificial system; Influence of Microbes on the Earth's Environment and Inhabitants; Ecological impacts of microbes; Symbiosis (Nitrogen fixation and ruminant symbiosis)Microbes and Nutrient cycles: types of cycles, Microbial communication system; Quorum sensing

UNIT-IIIMicrobiology of Waste Water Treatment: Waste water collection, treatments – physical, chemical and biological process, aerobic and anaerobic process, activated sludge, treatment schemes for waste waters of dairy, distillery, tannery, etc industries.Bioremediation: Introduction, constraints and types of Bioremediation, Biostimulation, Bioaugmentation, in situ, ex situ, intrinsic & engineered bioremediation, Solid phase bioremediation - land farming, prepared beds, soil piles, Phytoremediation.

UNIT-IVGlobal Environmental Problems: Ozone depletion, UV-B, green house effect, acid rain, their impact and management. Novel Methods for Pollution Control: Vermitechnology, waste water treatment using aquatic plants, root zone treatment, aiming for biodegradable and ecofriendly products.

COURSE OUTCOMES: Students will be able to understand the basic components of environment. Students will acquire board knowledge of microbial interactions in air, water and

soil.


TEXT / REFERENCE BOOKS: Environmental Biotechnology by S. K. Agarwal Biodegradation & Bioremediation (1999), Martin Alexander, Academic press. Stanier R. Y., Ingram J.L., Wheelis M.L., Painter R.R., General Microbiology, McMil-

lan Publications, 1989. Foster C.F., John Ware D.A., Environmental Biotechnology, Ellis Horwood Ltd.,

1987.


DBT207 BOTANY-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-III



: 3 Hours

COURSE OBJECTIVES: To understand the morphology, structure and importance of the plants. To acquaint the students about the various life supporting processes in plants.

UNIT -IPlant structure and Development: Structural organization and function of plant cell Plant growth, differentiation, organogenesis, morphogenesis, Programmed cell death, aging and senescence and environmental influences such as light, temperature, and gravity, cell division and elongation, seed germination, senescence, and plant tissue culture.

UNIT -IIPlant physiology: Photosynthesis: brief history, pigments, mechanism of light absorption and energy transfer, Light and dark phase of photosynthesis, PSI & PSII e-transport, ATP synthesis C3 C4 & CAM Photorespiration. Solute transport: Transport of inorganic and organic components, transport pathways-xylem and phloem

UNIT -IIIPlant Hormones: Plant hormones- Auxin, Gibbrellins, Cytokinins, Ethylene, Abscissic acid and Polyamines. Ethylene in plant growth, development, and senescence; Polyamines as endogenous growth regulators; Gibberellins and plant cell elongation; Hormonal regulation of apical dominance; Role of hormones in photosynthate partitioning and seed filling; Role of hormones during seed development. Role of hormones in tissue culture and micropropagation.

UNIT- IVSecondary Metabolites: Introduction to alkaloids, phenolics, plant terpenes, phytoalexins, sesquiterpenes and sterols.Biosynthetic pathway of secondary metabolites, tracer techniques.

COURSE OUTCOMES: The course will enable students to know the plant structure. Understand the importance of nutrients and photosynthesis in plants.


TEXT/REFERENCE BOOKS: Plant Biology by Allison Smith. Botany: An Introduction to Plant Biology by James D. Mauseth. Plant Biochemistry -Hans-Walter Heldt in cooperation with Fiona Heldt. Plant Physiology by Taiz Zeiger.


DCH215 CHEMISTRY-IIIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-III



: 3 Hours

Unit I

Microwave Spectroscopy: Basic Introduction, Electromagnetic radiation, interaction of electromagnetic radiation with matter, regions of the Spectrum, the width and intensity of spectral transitions, Resolving power. Basics of spectroscopy. The rotation of molecules, rotational spectra of rigid diatomic molecules, intensities of rotational spectral lines, isotopic effect, non-rigid rotator, spectra of polyatomic linear molecules and symmetric top molecules.

Unit II Electronic Spectroscopy: Electronics Spectra: Electronic spectra of diatomic molecules, vibrational course structure, and rotational fine structure of electronic band, the Frank-Condon principle, intensity of vibrational electronic band, dissociation energy, organic charge transfer complexes.

Unit III

UV Spectroscopy: Types of electronic transitions, λmax, Chromophores and Auxochromes, Bathochromic and Hypsochromic shifts, Intensity of absorption; Application of WoodwardRules for calculation of λmax for the following systems: Conjugated dienes: alicyclic, homoannular and heteroannular; Extended conjugated systems dienes; distinction between cis and trans isomers.

Unit IV

IR Spectroscopy: Fundamental and non-fundamental molecular vibrations; IR absorption positions of O, N and S containing functional groups; Effect of H-bonding, conjugation, resonance and ring size on IR absorptions; Fingerprint region and its significance; application in functional group analysis.


Books Suggested: 1. Physical Chemistry by P.W. Atkins 7th Edn. (1994). 2. Physical Chemistry by I.N. Levine 4h ed. (1993). 3. Physical Chemistry by Donald C. McQuarre (1983). 4. Introductory Quantum Chemistry by A.K. Chandra (1988). 5. Fundamentals of Spectroscopy by C.N. Banwell 4th Edn.(1994). 6. Molecular Quantum Mechanics by P.W. Atkins and R. S. Friedman,Oxford University Press, 2004. 7. Introduction to Nuclear Chemistry by H.J.Arnikar, New Age Publishers (1981) 8. Fundamentals of Radiochemistry by D.D.Sood, A.V.R.Reddy and N.Ramamoorthy, Indian Association of Nuclear Chemists and Allied Scientists (2000)

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsory and comprising short answer type questions of equal marks spread over the whole syllabus. The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.

DBT209 BIOCHEMISTRY AND MICROBIOLOGY LAB B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-III



: 3 Hours

COURSE OBJECTIVES: Understand principle, theory and calculation of each experiment. Understand the applicability of the biochemical and microbiological methods.

List of Practical:

1. Preparation of normal, molar solutions and percent solutions and determination of their pH.

2. Determination of pKa of acetic acid and glycine.3. Qualitative tests for carbohydrates and lipids.4. Qualitative tests for proteins and nucleic acids. 5. Preparation of casein from milk and determination of its isoelectric point.6. To study parts & use of a compound microscope.7. To prepare & sterilize culture media: Nutrient Agar, Potato dextrose Agar.8. Subculturing of microbial cultures: Preparation of agar slants andagar deep tubes.9. To perform Gram staining of the given bacterial culture.10. To perform Lactophenol cotton blue staining of the test fungi11. To isolate a pure culture of bacteria by streak plate and spread plating method.

COURSE OUTCOMES: Get first-hand experience that will coincide with what is taught in the lecture

portion of the class. Acquire and demonstrate competency in laboratory safety and in routine and

specialized laboratory skills.


TEXT / REFERENCE BOOKS: Introductory Practical Biochemistry (2014) .,S.K. Sawhney & R. Singh (Eds)

Published by Narosa Publishing House,ISBN 10: 8173193029 / ISBN 13: 9788173193026.Practical Biochemistry (1990) David Plummer. Tata Mc-Graw Hill

Biochemical Methods (1996) Sadasivam& Manickam New Age International (P) Ltd.

Microbiology- A lab manual, ed. Cappuccino and Sherman, 4 th Edition, Addi-son Wesley California, USA, 1999.

Experiments in microbiology, plant pathology,tissue culture & mushroom production technology ed. Aneja K.R, New Age International publishers New Delhi.

Note: The students will be required to perform 08 experiments/ exercises from the above list and the other two experiments may be designed by the department based on the related theory course

DCH217 CHEMISTRY LAB-IIIDual Degree B.Sc. (Hons)-M.Sc. Semester-III

Credits: 1.503 Hrs/week External Marks: 30Duration of Exam: 03 Hrs Sessional Marks: 20

List of Experiments:

Study the hydrolysis of methyl/ethyl acetate in presence of hydrochloric acid. Study saponification of methyl/ethyl acetate by sodium hydroxide solution using

same initial concentration of both the reactants. Determination of heat of neutralization

(i) Strong acid and strong base (ii) Strong base and weak acid

One step organic preparations Estimation of (i) Mg2+ (ii) Zn2+ by complexometric titrations using EDTA. Estimation of total hardness of a given sample of water by complexometric titra-

tionNote: The department can change any experiment depending on the availability of chemicals.

Books Suggested:

1. Vogel’s Textbook of Quantitative Analysis, revised, J. Bassett, R. C. Denney, G.H. Jeffery and J. Mendham, ELBS.

2. Vogel’s Textbook of Macro and Semimicro Qualitative Inorganic Analysis, revised, G. Svehla, Longman.

3. Christian, G. D., Analytical Chemistry, 6th Ed., John Wiley & Sons, Inc. (2004).

4. Skoog, D. A., West, D. M., Holler, R. J &Nieman, T. A. Principles of Instrumental Analysis Saunders Golden Sunburst Series (1997).


5. Willard, H. H., Merritt, L. L., Dean, J. A. & Settle, F. A. (Eds.) Instrumental Methods of Analysis - 7th Ed., Wadsworth Publishing (1988) ISBN 0534081428

DBT211 SEMINAR-IB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester III

L P Credits Class Work : 50 Marks- 2 1 Examination : -

Total : 50 Marks

COURSE OBJECTIVES: To learn how to carryout literature search To learn the art of verbal communication with the help of modern presentation

techniques

A student will select a topic in emerging areas of Biotechnology and will carry out the task under the observation of a teacher assigned by the department.

He/ She will give a seminar talk on the same before a committee constituted by the chairperson of the department. The committee should comprise of three faculty members from different specializations. The teacher associated in the committee will be assigned 2 hours teaching load per week.However, guiding students’ seminar will not be considered towards teaching load.

The students will be required to submit a seminar report in a prescribed format as per the university guidelines.


DBT202 INTRODUCTION TO GENETICS & MOLECULAR BIOLOGY B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IVL P Credits Class Work : 25 Marks4 - 4 Examination : 75 Marks


: 3 Hours

COURSE OBJECTIVES: The objective of this course is to introduce to the undergraduate students the ba-

sic concepts of inheritance; chemical nature of the genetic material; replication, mutation & repair of the genetic material; and finally the expression of gene and its regulation.

UNIT-IHeredity, Linkage, Crossing Over and Mapping: Concept of inheritance, Mendel and his experiments, mono- and di-hybrid crosses, modifications of standard Mendelian ratios, epistasis, incomplete and co-dominance, lethal genes, multiple alleles; Sex-Linkage: sex-linked, sex-limited and sex-influenced inheritance; Linkage, crossing-over and genetic mapping: gene mapping by tetrad analysis, three-point test crosses, andsomatic cell hybridization.

UNIT-IIGenetic Material-Chemical nature,Replication, Mutation and Repair: DNA and RNA as the genetic material - experimental evidences, packaging/ genomic organization of genetic material in prokaryotes and eukaryotes; DNA Replication: mechanism of DNA replication in prokaryotes and eukaryotes, replication in phages and retroviruses; Gene mutation: spontaneous and induced mutation, physical and chemical mutagens, tautomeric shifts,chromosomal aberrations-variations in chromosome structure & number; DNA repair mechanisms.

UNIT-IIIGene Expression- Transcription and Translation: Transcription in prokaryotes and eukaryotes- initiation, elongation & termination, role of RNA polymerases, sigma factor, transcription factors,promoters and enhancers in transcription; post-transcriptional processing of rRNA, tRNA and mRNA- poly-A tailing, 5’-capping, nuclear splicing, RNA editing; Translation: Genetic code and wobble hypothesis, translational mechanism in prokaryotes and eukaryotes, post - translational modifications- a brief account.

UNIT-IV


Regulation of Gene Expression: Regulation of gene expression in prokaryotes: operon concept, lac, trpandara operon, catabolite repression, attenuation, gene regulation in eukaryotes- methylation & acetylation, hormonal control of gene expression, RNA silencing.COURSE OUTCOMES:

This course will help the students gain the knowledge required for understanding the basic concepts of genetics & molecular biology.

TEXT/ REFERENCE BOOKS: Genetics, ed. Russell, P.J. 1998, Addison Wesley Longman, Inc. California, U.S.A. Genetics, ed. Brooker. R.J. 1999, Analysis and Principles. AW. Longman, Inc, Cali-

fornia Basic Genetics, ed. Miglani, G.S.2000. Narosa Publishing House, New Delhi. Principles of Genetics, Gardner, Simmons and Snustad, John Wiley & Sons. Concepts of Genetics, W.S. Klug, M.R. Cummings, C.A. Spencer and M.A. Palladino.

Pearson Publications. Molecular Biology of Cell, ed. Bruce Alberts, James D.Watson, Garland Publising


DBT204 INTRODUCTION TO IMMUNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IV



: 3 Hours

COURSE OBJECTIVES: To review basic concept of infection and immunity. To study the mechanism of microbial pathogenesis & immune mechanisms.

UNIT-IIntroduction to immune system: History, Immunity types, Innate immunity(Mechanical & chemical Barriers etc.) , Inflammation, Acute phase proteins, Toll like Receptors, Factors affecting Innate immunity, Adaptive Immune Response, Active and Passive Immunity, and its types ( Natural and Artificial). Introduction to Humoral, Cell mediated Immune Response and Complement system.

UNIT-IICells,organs of immune system& Antigens: Cells of the immune system: Hematopoiesis and its regulation..Organs- Primary Lymphoid organs and Secondary Lymphoid Organs Antigens: General properties of Antigens (Size, complexity, Route of entry, degree of relatedness), B cell & T cell epitopes, Super antigens, Haptens and Adjuvants.

UNIT-IIIHumoral Immune Response: Antigen processing and presentation [MHC –Brief account], Thymus dependent and Thymus Independent Antigen, B cell activation and development, Primary and secondary Humoral Immune Response, significance of TH cells in Humoral response. Effector response of antigen elimination.Antibodies: Introduction, Structure, Classes of Immunoglobulins, Effector functions, Antibodies as Approved in 12th meeting of Academic Council (1st Year and 13th meeting of Academic Council (2nd Year)

Antigens.Antigen- Antibody interactions- Immunodiffusion, Immunoelectrophoresis, ELISA, RIA.

UNIT-IVCell mediated Immunity:Lymphocytes and cell mediated response, Activation of Tc lymphocytes and generation of cytotoxic T lymphocytes, Mechanism of killing of foreign molecules by cytotoxicity. Cell mediated killing by natural killer cells, Antibody dependent cell mediated toxicity. Delayed type hypersensitivity.

COURSE OUTCOMES: Students will have an overall concept of disease and immune response Students will be able to analyse the methods of disease causation and thus pre-

vention

TEXT / REFERENCE BOOKS: Essentials of Immunology, ed. Ivan Roitt etal. Blackwell Scientific Publications, Ox-

ford. Fundamentals of Immunology, ed. Paul WZ, Raven Press, New York. Immunology, ed. Kuby etal W.H. Freeman &Company, New York. Microbiology ,ed. Prescott etal. 2007,Mc Graw Hill, USA.


DBT206 INSTRUMENTATION IN BIOTECHNOLOGYB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology



: 3 Hours

COURSE OBJECTIVES: Enable the students to have a deep knowledge on the techniques used in biotech-

nology. Enable the students to get an insight on the usage of various techniques and their

application in research.UNIT-I

Microscopy: Principles of microscopy, Light, dark field, fluorescent,transmission and Scanning electron microscopy, Confocal microscopy, microtomy and analysis and measurement of images.Introduction to chromatographic techniques: Basic principles of chromatographic separations. Column, thin layer, Paper, Normal phase and reverse phase chromatography, Ion-exchange, Affinity and Gas Chromatography, High performance liquid chromatography (HPLC)

UNIT-IIElectrophoretic techniques: Theory and application of polyacrylamide and agarose gel electrophoresis, electrophoresis of protein and nucleic acids, Capillary electrophoresis.Centrifugation techniques: Introduction, Basic principle of sedimentation, Centrifuges and their uses, safety aspects in the use of centrifuges. Density gradient and analytical centrifugation


UNIT-IIISpectroscopic techniques: Theory and application of UV-VIS, IR, NMR, Fluorescence, Atomic absorption spectroscopy; X-ray diffraction. Introduction to mass spectroscopy

UNIT-IVRadioisotopic techniques: Introduction to radioisotopes, detection, measurement and uses of radioisotopes, counting efficiency and autoradiography, biotechnological applications

COURSE OUTCOMES: Know basic principles of different analytical techniques used in biotechnology Understand the application of different analytical techniques used in biotechnol-

ogy.

TEXT / REFERENCE BOOKS: Freifelder, D. (1982) Physical Biochemistry 2 nd edition, W.H. Freeman and Co.,

N.Y. USA. Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. 6th Edi-

tion. JohnWiley&Sons.Inc. De Robertis, E.D.P. and De Robertis, E.M.F.(2006)Cell and Molecular Biology.8th

edition.Lippincott Williams and Wilkins, Philadelphia. Cooper, G.M. and Hausman, R.E. (2009) The Cell: A Molecular Approach. 5th edi-

tion. ASM Press & Sunderland, Washington, D.C.; Sinauer Associates, MA. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009) The World of the

Cell.7thedition. Pearson Benjamin Cummings Publishing, San Francisco.


DBT208 ZOOLOGY-II B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IV



: 3 Hours

COURSE OBJECTIVES: To study the taxonomic principles and phylogeny of animals. To understand the morphology, structure and importance of the organisms.

UNIT- IGeneral account: Chordata; Hemichordata- Characteristics, Classification and Affinities and systematic position of hemichordata. Link between chordates and Non-chordates.Uchordata: Characteristics, Classification and Affinities, Herdmania. Cephalochordata: Characteristics, Classification and Affinities, Branchiostoma.Vertebrata; Characteristics and Classification, Pisces: Characteristics and Classification

UNIT- IIAmphibia: General Characteristics, Classification with suitable examples. Amphibians in ecological hotspots to be emphasizedReptilia: General Characteristics and Classification with emphasis on terrestrial adaptations.


Aves: General Characteristics and Classification. Birds as bio-indicators and threat to vultures to be emphasized.Mammalia: General Characteristics and Classification with suitable examples

UNIT- IIIVertebrate integument and its derivatives; Skeletal system- form, function, body size and skeletal elements of the body, comparative account of joint suspensorium, vertebral column; Digestive system- dentition, stomach, digestive glands. Blood and Evolution of Heart.

UNIT- IVRespiratory system-characters of respiratory tissue, external and internal respiration; comparative account of respiratory organs.Nervous system- comparative anatomy of brain and spinal cord.Nerves- cranial, peripheral and autonomic nervous system.Brief account of sensory organs.

COURSE OUTCOMES: Understand body construction from unicellular to multicellular organisms & its

evolution. Able to understand the social organization in different organisms.

TEXT/REFERENCE BOOKS: Modern Text Book of Zoology-Invertebrates by Kotpal R. L. A Text Book of Zoology. Vol II by Parker &Hasewell. Life of Vertebrates by Young J. Z. A Manual of Zoology. Vol II by Ayyar E.


DCH214 CHEMISTRY-IVB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology



: 3 Hours

Course Objectives: This course provides the discussion of different methods of chemical analysis. Along with the elementary knowledge of statistical aspect of analysis, theoretical background and principles involved in chromatography, ion-exchange and solvent extraction techniques are included in this course.Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsory and comprising short answer type questions of equal marks spread over the whole syllabus. The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.

UNIT -IAnalytical Chemistry


Sources of errors in chemical analysis, classification of errors, precision, accuracy, mean, median and mode, standard deviation, statistical evaluation and interpretation of results in analytical chemistry (with numericals).

UNIT -IIChromatographyClassification of chromatographic methods, chromatographic terminology - R f value, partition co-efficient, dynamics of chromatography, basic principles of adsorption and partition chromatography, applications.

UNIT -IIIIon - ExchangeCharacteristics of ion-exchangers, mechanism of ion-exchange, ion-exchange equilibra, plate theory for ion-exchange, techniques of ion-exchange and applications of ion exchange for separations.

UNIT -IVSolvent ExtractionBasic principles of solvent extraction, classification and mechanism of extraction, extraction equilibria, techniques of extraction and applications in analytical chemistry.

Books Suggested



3. Christian, G. D., Analytical Chemistry, 6th Ed., John Wiley & Sons, Inc. (2004). 4. Skoog, D. A., West, D. M., Holler, R. J &Nieman, T. A. Principles of Instrumental Analysis

Saunders Golden Sunburst Series (1997). 5. Willard, H. H., Merritt, L. L., Dean, J. A. & Settle, F. A. (Eds.) Instrumental Methods of

Analysis - 7th Ed., Wadsworth Publishing (1988) ISBN 0534081428

Note: The question paper will consist of nine questions. The candidates will be required to attempt five questions in all. The Question No. 1 will be compulsory and comprising short answer type questions of equal marks spread over the whole syllabus. The candidate shall attempt four more questions selecting at least one from each Unit. All questions will carry equal marks.

DBT210 IMMUNOLOGY AND MOLECULAR BIOLOGY LABORATORY B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IV



: 3 Hours

COURSE OBJECTIVES: To impart practical knowledge and methods in molecular biology. To understand the implications of the scientific and technical approaches in

immunological techniques.

LIST OF EXPERIMENTS:1. Introduction to Molecular Biology laboratory and use of basic equipments.2. Handling and disposal of hazardous reagents- acids, carcinogenic chemicals eg

EtBr etc.


3. Preparation of commonly used molecular biology buffers.4. Isolation of genomic DNA from bacterial cells.5. Quantification of DNA.6. Immunization of Laboratory Animals.7. Collection of blood and preparation of serum from the given blood sample.8. To prepare blood film and identify the blood cells.9. Lymphoid organs and their microscopic organization.10. To perform Immunodiffusion by Mancini and Ouchterlony techniques.11. To identify the human blood groups A, B, AB, O& Rh factor in the test population.12. To perform differential leucocyte count of the blood sample.

COURSE OUTCOMES: Technical know-how the basic molecular biology techniques. Proficiency in designing and conducting experiments of immunology.

TEXT/REFERENCE BOOKS: Molecular Cloning – A laboratory manual, ed. J. Sambrook and D.W. Russell, 2001, Cold Spring Harbor laboratory Press, New York Molecular Biology-Principles and practices, ed. Priyanka Siwach and Namita Singh.2009, Laxmi Publications, New Delhi Practical Immunology by G.P Talwar. Practical Immunology by Hudson & Hay.



DCH216 CHEMISTRY LAB-IV B.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester-IV

L P Credits Class Work : 20 Marks- 3 1.5 Examination : 30 Marks


: 3 Hours

LIST OF EXPERIMENTS: Determine the strength of strong acid by conductometric titration with strong

base. Determine the strength of weak acid by conductometric titration with strong base. Determine the strength of strong acid by pH-metric titration with strong base. Determine the strength of weak acid by pH-metric titration with strong base. Detection of functional group in the given organic compound To verify Beer-Lambert law for KMnO4, K2Cr2 O7 and determine the concentration

of the given KMnO4 , K2Cr2 O7 solution. Separation and estimation of Fe(II) (Estimation by colorimetrically) Preparation of (a) Copper tetraamine complex [Cu(NH3)4]SO4

(b) Mercuric tetrathiocyanatocobaltate (II), Hg[Co(SCN)4]


Books Suggested



3. Christian, G. D., Analytical Chemistry, 6th Ed., John Wiley & Sons, Inc. (2004).

4. Skoog, D. A., West, D. M., Holler, R. J &Nieman, T. A. Principles of Instrumental Analysis Saunders Golden Sunburst Series (1997).

5. Willard, H. H., Merritt, L. L., Dean, J. A. & Settle, F. A. (Eds.) Instrumental Methods of Analysis - 7th Ed., Wadsworth Publishing (1988).



DBT212 SEMINAR-IIB.Sc. (Hons)-M.Sc. Dual Degree in Biotechnology

Semester IV

L P Credits Class Work : 50 Marks- 2 1 Examination : -

Total : 50 Marks

COURSE OBJECTIVES: To learn the art of verbal communication with the help of modern presentation

techniques

A student will select a topic in emerging areas of Biotechnology and will carry out the task under the observation of a teacher assigned by the department.

He/ She will give a seminar talk on the same before a committee constituted by the chairperson the department. The committee should comprise of three faculty members from different specializations. The teacher associated in the committee will be assigned 2 hours teaching load per week.`However, guiding students’ seminar will not be considered towards teaching load.

The students will be required to submit a seminar report in a prescribed format as per the university guidelines.


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