chemical imaging of lipid domains by high-resolution secondary ion mass spectrometry (hr-sims)
DESCRIPTION
Chemical Imaging of Lipid Domains by High-Resolution Secondary Ion Mass Spectrometry (HR-SIMS) Mary Kraft † , Peter Webber § , Ian Hutcheon § Marjorie Longo ‡ & Steven Boxer † , † Stanford University, § Lawrence Livermore National Lab & ‡ UC Davis Stanford MRSEC, DMR-0213618. - PowerPoint PPT PresentationTRANSCRIPT
Chemical Imaging of Lipid Domains by High-ResolutionSecondary Ion Mass Spectrometry (HR-SIMS)
Mary Kraft†, Peter Webber§, Ian Hutcheon § Marjorie Longo‡ & Steven Boxer†, †Stanford University, §Lawrence Livermore National Lab & ‡UC Davis
Stanford MRSEC, DMR-0213618
SiO2 on Si
12C15N-
from15N-DLPC
12C15N-
+13C1H-
Phase separated supported lipid bilayers were chemically imaged by HR-SIMS performed with the NanoSIMS 50 (Cameca Instruments). A focused Cs+ ion beam is rastered across the sample, extensively fragmenting the surface components. Secondary ions of up to 5 different masses are simultaneously detected and a lateral resolution of 50 nm can be achieved. In our experiments, a unique stable isotope was selectively incorporated into each membrane component, and the intensity and location of the isotopically enriched secondary ions were used to create a component-specific image of the phase separated lipid bilayer.
AFM
13C1H-
from13C18-DSPC
DLPC (Tm = -5 ºC)fluid phase
15N-DLPC 12C15N-
DSPC (Tm = 55 ºC)gel phase
13C18-DSPC 13C1H-