chem 217 metallobiochemistry t/th 8-9:20am, w 12-12:50 pat farmer psi 319 x6079, [email protected]...
TRANSCRIPT
Chem 217
Metallobiochemistry T/Th 8-9:20AM, W 12-12:50
Pat Farmer
PSI 319
x6079, [email protected]
•Office hours:– MW 8-9AM and by appointment
OverviewOverview
Emphasis will be on the structural motifs of metalloprotein centers and their associated reactivities. Course material will offer broad exposure to multidisciplinary topics and methodologies. Prerequisites include undergraduate inorganic chemistry, physical or analytical chemistry, some knowledge of biochemistry. The major goal of this course is to provide you with the background and tools necessary for a critical appraisal of current bioinorganic topics in the literature. One research project will be required, given as a 3-5 page
report at the end of the term.
Understanding Protein Structure
Looking at Protein Images– what do those squiggles mean
Downloading Structures
from the PDB– something you can do
through out the course
Imaging an Active Site in the Modeling Facility– make an image and see it on the web
Bioinorganic Motifs• Hemeproteins,
– the colors of life • FeS clusters,
– from a time before oxygen• Cu proteins,
– versatile redox centers• Zn, Ca
– signal transducers
Heme Proteins: The colors of life
– “Why the bloode is red and the grasse green, these are mysteries no Man can fathom” John Dunne 17xx?
heme are ubiquitous
– one structure/many functions
– function controled by • oxidation state• ligand binding
FeS clusters: an ancient motif
ubiquitous
in mitochondria self-assembly
of clusters optimized for fast electron transfer
– minimum reorganization– electron delocalization
Cu Proteins:
Controlled Redox– oxygen binding and activation– cyt c oxidase, THE ultimate
electron acceptor
Radical Generation – tyrosinase
Signal Transduction
Ca binding proteins– EF hand motif
• intercellular
signal amplification
Zn binding proteins– Zn fingers– DNA binding
• gene regulation
Procedures
Highlight any procedural differences from regular projects of this type
Discuss requirements, benefits, and issues of using new procedures
List location or contact for procedures document (or other related documents) here
FOR MORE INFO...
Schedule
Review high-level schedule milestones here
List location or contact for detailed schedule (or other related documents) here
FOR MORE INFO...
Current Status
High-level overview of progress against schedule– On-track in what areas– Behind in what areas– Ahead in what areas
Unexpected delays or issues
Related Documents
marketing plan– location or contact name/phone
budget– location or contact name/phone
post mortem– location or contact name/phone
submit questions– location or contact name/phone