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Designed to deliver precise, robust results, the Cell DIVE™ multiplexed imaging solution is an integrated system for imaging 60+ biomarkers from one tissue sample at a single-cell level. The solution consists of a fully customizable assay design with tissue-preserving staining protocol and a precision-engineered imager with intuitive, scalable acquisition and analysis software. These elements come together to form a multiplexing tool tested over 10 years to deliver accurate results across multiple studies (Fig 3).

The Cell DIVE multiplexed imaging solution is:

• Customizable: design and configure experiments exactly how you want

• Precise: collect the data valuable to you, and trust in your results

• Proven: years of experience plus three tested and refined technologies are merged into one solution

Cell DIVE multiplexed imaging solution

Fig 1. The Cell DIVE multiplexed imaging solution is fully customizable and includes a precision-engineered imager with intuitive, scalabale acquisition and analysis software.

Fig 2. Cancer images obtained using the Cell DIVE multiplexed imaging solution.

Colon cancer

Lung cancer

Breast cancer

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The three-part characterization process tests for specificity, sensitivity, and compatibility with the Cell DIVE two-step antigen retrieval process, as well as antigen effects due to the dye inactivation solution. To date, over 2000 clones have been tested to create the list of 350+ validated antibodies. This list will save months of experiment set-up time and minimize project expenses related to antibody characterization, all while ensuring robust and accurate results.

Table 1. Validated antibody list.

Item Details

List of validated antibodies

14-3-3 sigma, 3R-Tau, 4E-BP1, 4E-BP1_pT37_pT46, 4R-Tau, ABCB1, ABCG2, Abeta40, Abeta42, ADRP, AIBC1, Akt, Akt_pS473, Albumin, ALDH1, ALDH1L1, Alix, ALK, AMACR, AML3, AMPH2, Amphiregulin, AMPK-beta1, Amyloid_beta, Ang2, Ang4, APAF-1, API3, APOE, APOE-E4, Apo-J, AQP1, AQP4, AR, Arc, Arg1, ARGEF17, ATM_pS1981, ATP5A, ATR, AuroraA, AuroraB, BACE1, Bak, Bax,Bcl-2, Bcl-xL, BDNF, Beclin1, beta-actin, Beta-Catenin, BID, Bim, B-III-Tubulin, BMI1, BOB1, BRCA1, C3, C4d, C9orf72_GA, C9orf72_GP, C9orf72_GR, CA9, Cadherin_pan, Cadherin-1, Cadherin-1, Cadherin-3, CALB1, Calretinin, CAMK2, Caspase-3, Caspase-3_cleaved, Caspase-6_cleaved. Caspase-9, CD10, CD11b, CD11c, CD137, CD15, CD16, CD163, CD20, CD200R, CD206, CD21, CD274, CD3, CD30, CD31, CD32, CD34, CD38, CD39, CD4, CD44, CD44v6, CD45, CD45RA, CD45RO, CD45RO_CC, CD5, CD56, CD64, CD68, CD79, CD8, CD83, Cdc25b, Cdc25c, Cdc7,, CDCP1, Cdk6, Cdx2, CEACAM5, cFos, Chk2_pT68, c-IAP1, c-IAP2, Claudin-1, cMet, cMet_pY1234, cMet_pY1349, c-Myc, CNPase, Cofilin, Collagen IV, Cox2, Creb, Creb_pS133, CSF1R, CX3C1R, Cyclin A2, Cyclin B1, Cyclin D1, Cyclin E1, Cytokeratin 5_6, Cytokeratin 8_18, Cytokeratin_AE1, Cytokeratin_AE1_AE3, Cytokeratin_AE3, Cytokeratin_PCK26, Cytokeratin-15, Cytokeratin-18, Cytokeratin-19, Cytokeratin-20, Cytokeratin-5, Cytokeratin-6, Cytokeratin-7, DAPPER1, DBF4, Desmin, DJ-1, DLL4, Doublecortin, DPYD, DR5, DUSP5, e1F4B, e1F4B_pS422, E2F1, eEF2K, Eg5, EGFR, EGFR_pY1068, eIF4E, eIF4E_pS209, eIF4G, EpCAM, EphB2, Epiregulin, ER, ERG, Erk1_2, Erk1_2_pT202_pY204, EZH2, FADD, FADD, FAK, FAP, FAS, FGFR1, FGFR2, FGFR3, Fibronectin, FKBP5, FLIP, FOXO1, FOXO3a, FOXP3, FSP, FTH1, FTL, FUS, G6PD, GADD45B, Galectin-3, GAPDH, GAP43, GATA3, GDNF, GFAP, Gli1, Glut-1, Granzyme_B, GRP78, GSK-3a_pS21, GSK3b, GSK-3B_pS9, H2AX, Hepsin, Her2, Her2_pY1221_pY1222, Her3, Her3_pY1289, Her4, HIF1- alpha, Histone H3_pS10, HK2, HLA_class1, HSP27, HSP27_pS15, HSP27_pS78, HSP27_pS78, HSP27_pS82, HSP70, HSP90, HTF9C, HUD, Iba1, IDH1, IGF1R, IHH, IL6, ILK, iNOS, IR, IRS1, ITGA6, Jagged, Ki67, LAG3, Laminin_A_C, LAMP1, LC3A, LC3B, LDH-A, Lgr5, Mage-A1, Mage-A2, MAP2, MAPKAPK2, MAPKAPK2_pT334, MBP, MCL1, MCM2, MEK5, MHC_II, MLH1, MLKL, MNK_pT197, MPO, Msh2, Msh6, MSR1, MsrA, mTor, mTor_pS2448, MUC1, MUC2, NAKATPase, NapsinA,Ncadherin, NDRG1, NDRG1_pT346, Nestin, NeuN, Neuregulin-3, Neuregulin-4, NF, NFkb_p105_pS933, NFKb_p65_pS276, NFP, NG2, NKG2D, NKX3-1, Notch1, Notch3, NR1, NRP1,NRP2, Oct-2,Oct-2, Osteonectin, p120_catenin, p21, p38MAPK_pT180_Y182, p53, p62, p63, p70S6K_pT389, Parvalbumin,Pax-5, PD1, PDCD4, PDCD4_pS67, PDGFR-alpha, PDGFR-beta, PDK1, PDK1_pS241, Peripherin, PGE2, PGP9.5, PGRN, PHF-tau_pS202_pT205, PHF-tau_pThr231, Phosphotyrosine, PI3K_p110a, PKM2, PLAC8, PLK-1, PMS2, PNDRG1, pNF, PR, PRAS40, PRAS40_pT246, PRKCA, PRKCB, PSA, PSD95, PSMA,PSP94, PTEN, PTP4A2, Rab5, RB, RB_pS608, RB-pS780, RIP1, RIP3, Robo2, Ron, RRM1, RSK, RSK1_pT359_363, S100-A10, S100A4, S100-A7, S100-B, S6, S6_pS235_pS236, SDHA, Semaphorin 4D, Serpin-B5, SFRP4, SKP2, SLC7A5, SMA, Smac, Smad3, Smad3_pS423_S425,Smad4, SOCS-2, SorLA, Sox2, Spinophilin, SR-2B, Src_kinase, Stat3, Stat3_pY705, Stat5, Stat5_pY694, Stathmin, Survivin, Synaptophysin, Synuclein_a_pS129, Tau, TauC3, TDP-43, TDP43_pS409/410, TDP43_pS409/410, Tenascin, TGFB1, TGFBRAP1, ThymidylateSynthetas, Tie2, TIGAR, TIGAR, TIM3, TIMP4, TKTL1, TMPRSS2, TOP2a, TRADD, Transferrin_R_I, TRIM29, TRKB, TSC2_pT1462, TSPO, TTF-1, TYROBP, Ubiquitin, UBQLN2, VACV, VEGF-A, VEGF-C, VEGFR1, VEGFR2, VEGFR3, Vimentin, vonWillibrand_Factor, Vps35, Wnt5A, XBP1, xCT, Zeb1

Fig 3. Cell DIVE multiplexed imaging solution.

Cell DIVE licenseThe Cell DIVE license is an integral part of the overall Cell DIVE solution and includes access to the validated antibody list, antibody validation protocol, patented staining protocol, and Cell DIVE acquisition software.

Validated antibody list

The Cell DIVE license provides access to an extensive list of primary and directly conjugated antibodies that have been rigorously characterized to provide robust multiplexing results. Biomarker panels are completely customizable with Cell DIVE — choose your panel from the list of 350+ validated antibodies, or use the comprehensive characterization protocol to validate new antibodies.

Cell DIVE multiplexed imaging solution

Validated antibodies

Patented workflow

Acquisition software

Automated imager

Analysis software

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Patented staining protocol

The Cell DIVE staining protocol is based on a patented chemical dye inactivation process compatible with certain cyanine dyes. After staining and imaging, the dye inactivation solution quenches the dye molecules in the sample, enabling the sample to be probed repeatedly with new dye-conjugated antibodies. The gentle dye inactivation solution does not damage tissue, preserving tissue and antigen integrity over multiple staining rounds.

Cell DIVE acquisition software suite

Cell DIVE acquisition software is a purpose-built multiplexing software suite that integrates image acquisition, imaging correction and stitching, quality control, and workflow management for a seamless multiplexed imaging experience.

The Cell DIVE software suite gives you the flexibility to choose which regions of interest to image. Images can be acquired from the whole slide or from multiple smaller regions of interest, each comprised of many singular fields of view. For each field of view (FOV), channel, and Cell DIVE imaging round, raw images are saved along with post-processed stitched images.

Image acquisition is controlled with Cell DIVE acquisition software v1.0 or higher and Mx Workflow software v1.0 or higher. These software packages enable the following functionalities:

Purpose-built software suite

• At the end of an imaging round, a corrected, stitched image is automatically created — no user intervention required.

• Because multiplexed imaging is all the software does, there is a balance between flexibility and ease of use. The user has just the right amount of choice, and most expected functions are done automatically.

• Fully integrated with the Cell DIVE imager and designed expressly for the Cell DIVE staining process, the Cell DIVE software brings all elements of the solution together for superb results.

Flexibility

• Choose your biomarkers, choose the number of biomarkers per round, and choose the number of rounds.

• Select a single FOV, a stitched area up to 45 mm × 20 mm, or any number or combination of the two for comprehensive sampling.

• Streamlined import to the HALO™ image analysis platform and TIFF file type compatibility with other image analysis software packages.

Accurate downstream single-cell analysis

• State-of-the-art automatic calibration routine and customized calibration plate allow for confident correlation of data across multiple samples, multiple studies, and multiple imagers.

• Fully integrated flat field correction, background glass subtraction, distortion correction, and autofluorescence removal result in high-quality, seamlessly stitched images.

• Patented image alignment routine using DAPI staining precisely overlays images from all imaging rounds for accurate spatial mapping.

Scalability

• Identify regions of interest in the first round, then for each subsequent round just load the sample and walk away — imaging proceeds automatically and you remain productive.

• Increase throughput by integrating robotic plate handlers into the workflow.

• Advanced calibration enables imaging of samples and correlation of data across multiple imagers to increase throughput.

Integrated multiplexed imaging management

• Barcode-based sample recognition enables progression through the multiplexed imaging workflow without user intervention.

• Manage concurrent studies with no interruption in workflow.

• Integrated quality control (QC) steps ensure successful imaging prior to moving on to the next protocol step.

Table 2. Cell DIVE staining and imaging process

Protocol step

Description Details

Initial sample preparation

Prepare formalin-fixed, paraffin-embedded tissue slides for imaging

Only performed once at the beginning of the Cell DIVE process for each slide

Includes slide baking, clearing, antigen retrieval, and blocking

2× tissue location

Acquire 2× DAPI overview image of entire slide

Identify what part of the slide to image at 10× for higher resolution region selection

10× region selection

Acquire 10× virtual H&E image(s)

10× images are acquired based on the regions selected in the 2× scan

Images are automatically corrected and stitched then opened in a viewer for user QC

Select regions of any shape or size — these regions will be used for all downstream imaging

20× auto-fluorescence imaging

Acquire 20× images for DAPI and all biomarker channels

Images are automatically acquired based on the regions selected in the 10× scan

Images are automatically corrected and stitched then opened in a viewer for user QC

Images are used for autofluorescence removal in biomarker imaging step

Biomarker staining

Stain sample with DAPI and up to four dye-conjugated antibodies

Pick antibodies from the validated antibody list, or validate your own

20× biomarker imaging

Acquire 20× images for DAPI and all biomarker channels

Images are automatically acquired based on the regions selected in the 10× scan

Images are automatically corrected and stitched then opened in a viewer for user QC

Dye inactivation

Incubate sample with chemical dye inactivation solution

Patented dye inactivation process quenches dyes without tissue damageReturn to 20× autofluorescence imaging to begin the next imaging and staining round for the next set of biomarkers

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Cell DIVE imagerEngineered for precision, speed, and sensitivity, the Cell DIVE imager is a purpose-built, fully-automated tissue imaging system. Building on the proven capability of the IN Cell Analyzer family of high-content imaging systems, the Cell DIVE imager is a robust automated imager for tissue samples. Multiple hardware and software innovations increase speed and sensitivity, allowing tissue slides to be imaged with confidence.

The Cell DIVE imager is supplied with 2×, 10×, and 20× objectives that fully support the Cell DIVE imaging workflow. Controlled with custom image acquisition software, the Cell DIVE imager enables 2D hyperplexed imaging of both coverslipped and non-coverslipped slides. Equipped with a five-channel solid-state illumination (SSI) source and a penta-band polychroic, the Cell DIVE imager supports imaging DAPI, Cy™2, Cy3, Cy5, and Cy7-like fluorophores.

IlluminationThe Cell DIVE imager has an SSI light engine with five independently controlled light sources that provides extremely bright and long-lasting illumination for fluorescence imaging. Exceptional stability over time and uniform output intensity for all biomarker channels1 ensures experimental reproducibility and quantitative results. Light is delivered to the sample through a 1.5 mm fiber optic cable that provides uniform illumination across the specimen.

1. Excepting the near-infrared (IR) channel: at near-IR wavelengths, higher output is required to compensate for lower camera quantum efficiency (QE).

Table 3. SSI general specifications

Parameter Specification

Wavelength switching time

< 1 ms

Optical fiber 1.5 mm diameter, 0.39 numerical aperture (NA)

Table 4. Optical power ratings for SSI module

Channel Minimum power* (mW)

Blue 85

Green 100

Orange 100

Far red 100

Near IR 150

* Power measured from the end of the fiber optic cable.

Filters and polychroicsFor optimal illumination efficiency, the Cell DIVE imager includes a penta-band pass polychroic mirror and five single band pass emission filters matched to the SSI channels.

Single band pass excitation filters Channel band

Band (nm)

Center wavelength (nm)

Bandwidth (nm)

Blue 379–401 390 22

Green 458–482 470 24

Orange 525.5–558.5 542 33

Far red 617–645 631 28

Near IR 710–750 730 40

Single band pass emission filters Channel band

Band (nm)

Center wavelength (nm)

Bandwidth (nm)

Blue 411–446 428.5 35

Green 495–512 503.5 17

Orange 574–599 586.5 25

Far red 664–690 677 26

Near IR 772–814 793 42

Polychroic beam splitter Channel band

Band (nm)

Center wavelength (nm)

Bandwidth (nm)

Blue EX 378–401 390 23

Blue EM 409–448 428.5 39

Green EX 457–483 470 26

Green EM 493–515 504 22

Orange EX 526–558 542 32

Orange EM 571–603 587 16

Far red EX 617–645 631 28

Far red EM 660–695 677.5 35

Near IR EX 711–750 730 39

Near IR EM 767–820 793.5 53

Fig 4. The Cell DIVE imager is an automated widefield imaging system for whole slide tissue analysis.

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ObjectivesThe Cell DIVE imager comes with a four-position motorized objective turret. Three objective lenses are routinely used in the Cell DIVE imaging workflow: 2× for overall tissue location, 10× for region selection, and 20× for high-resolution images to be used for downstream single-cell analysis. The standard 2×, 10×, and 20× objective lenses can be used for samples that must be imaged through the coverslip or glass slide. Additionally, an optional high numerical aperture (NA) 20× objective can be purchased explicitly for through-coverslip imaging.

Table 7. Specifications of available objectives

Magnification NA Optical

correctionsWD

(mm)Resolution

(nm)† Image through

Cover-slip

Glass slide

2×* 0.1 CFI Plan Apo Lambda

8.5 3050 √ √

10×* 0.45 CFI Plan Apo Lambda

4.0 678 √ √

20×* 0.70 CFI S Plan Fluor LWD

2.3–1.3 436 √‡ √‡

20× 0.75 CFI Plan Apo Lambda

1.0 407 √

Key: NA = numerical aperture; WD = working distance; CFI = chromatic aberration free infinity; Plan = flat field correction; Apo = apochromat, indicating color correction; S Plan = super plan; LWD = long working distance* Supplied as standard with Cell DIVE imager† Resolution values are calculated and represent maximum theoretical resolution at 500 nm.

Actual resolution values will vary based upon the wavelength, imaging conditions and sample characteristics.

‡ The correction collar on the 20× 0.70 NA objective can only be set in an optimized position for either through coverslip or through slide imaging. Switching between the two might require a service visit to adjust the correction collar.

StageThe Cell DIVE imager stage balances speed with accuracy and repeatability to enable precise imaging without sacrificing throughput.

Table 8. Stage specifications

X- and Y-axes Z-axis

Speed (mm/s) 60 2.8

Supported sample types 75 × 25 mm slides

Table 9. Sample imaging times*

Sample size Objective FOVs ChannelsExposure time (ms)

Imaging time (min)

Whole slide 2× 36 1 100 2

45 × 20 mm area

10× 646 2 10 17

20× 2584 2 10 79

20× 2584 3 10 110

20× 2584 4 10 135

20× 2584 5 10 167

Quantity 10 2.4 × 2.4 mm areas

20× 160 2 10 5

20× 160 3 10 6

20× 160 4 10 8

20× 160 5 10 10

* Imaging times are included as benchmarks; performance will vary based upon sample and protocol specifications. Imaging time does not include tilt check or auto-align procedures, which will add 1 to 5 minutes per acquisition.

Focus maintenanceThe Cell DIVE imager is equipped with both hardware and software autofocus features. The laser-based hardware autofocus utilizes an 850 nm laser to determine the interface between air and the bottom of the slide or coverslip. Software autofocus captures multiple images in different z-planes and determines the focus position with maximal contrast. Both hardware and software autofocus features are used automatically during Cell DIVE imaging to ensure in-focus signal is captured during image acquisition.

Table 10. Hardware autofocus specifications

Parameter Specification

Wavelength (nm) 850

Power (mW) 40

CameraA scientific complementary metal-oxide semiconductor (sCMOS) camera delivers the large FOV essential for multiplexed imaging, without sacrificing resolution. Additionally, the high quantum efficiency and low noise that are characteristic of sCMOS detectors ensure excellent image quality and maximum sensitivity. Finally, the 16-bit dynamic range allows confident detection of both bright and dim structures in the same image.

Table 11. Camera specifications

Parameter Specification

Image sensor sCMOS

Chip size 2040 × 2040 pixels

Sensor size 13.3 × 13.3 mm, 18.8 mm (diagonal)

Pixel size 6.5 × 6.5 μm

Readout speed 272.3 mHz

Cooling Air

Dynamic range 16 bit

Spectral range 370 to 1100 nm

Quantum efficiency Up to 82%

Full well capacity (e-) 30 000

Readout noise e- (median/rms) 0.9/1.4

Operating environment 10°C to 60°C; 10% to 80% humidity

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WorkstationThe Cell DIVE imager is supplied with an optimized workstation that controls image acquisition. The workstation runs Windows® 10 and includes a large hard drive for data storage. Cell DIVE Acquisition software is compatible with network associated storage devices and USB devices.

Table 12. Acquisition workstation specifications*

Parameter Specification

CPU Intel Xeon™ W-2145

Number of cores 8

CPU speed 3.7 GHz

RAM 64 GB

Primary hard disk 4TB 7200 RPM SATA

Network interface Intel X550-T2 10GbE Dual Port Ethernet

Operating system Windows 10 Pro 64 Workstations Plus US

* Workstation specifications are subject to change without notice.

Table 13. Monitor specifications*

Parameter Specification

Panel size (diagonal) 27 in

Aspect ratio 16:9

Optimal resolution 2560 x 1440 pixels

Contrast ratio (typical) 1000:1 static

Dynamic contrast ratio (max) 10 000 000:1

Brightness (typical) 350 cd/m2

Response time (gray to gray) 5 ms

Viewing angle 178° horizontal; 178° vertical

* Monitor specifications are subject to change without notice.

Sample cassettesThe Cell DIVE imager supports two sample cassettes to accommodate slide-based imaging. The dual slide cassette (Fig 5A) enables imaging of two coverslipped 75 × 25 mm microscope slides. The open well cassette (Fig 5B) allows for through-slide imaging of one microscope slide, eliminating the need for coverslipping and reducing potential tissue loss. One of each sample holder type is included with the Cell DIVE imager. Additional sample cassettes can be purchased separately.

System specificationsThe Cell DIVE imager is fully enclosed, enabling installation in a standard laboratory setting. The enclosure provides isolation from vibration and air flow fluctuations to create a stable imaging environment. The Cell DIVE imager fits on a standard laboratory bench.

Table 14. System specifications

Parameter Specification

Illumination modalities Widefield fluorescence

System enclosure Total system enclosure with laser interlock for safer operation

Width 112.8 cm / 44.4 in

Height 66 cm / 26 in

Depth 63.5 cm / 25 in

Weight 105.7 kg / 233 lb

Operating temperature Stable from 18 – 24° C (64 – 75° F)

Temperature fluctuation No more than ± 2°C over 4 h with an hourly variation of no more than 1° C

Relative humidity 10% to 80%, noncondensing

Heat output 500 W

Power requirements 100–127/200–240 VAC, 5A/2.5 A 50–60 Hz

Power consumption (max) 500 W

Overvoltage category II

Manufacturing site Cytiva, Issaquah, WA, USA

Fig 6. Dimensions of the Cell DIVE imager.

112.8 cm / 44.4 in

66

cm /

26 in

(A) (B)

Fig 5. Image of (A) dual slide cassette and (B) open well cassette.

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Fig 7. HALO software interface.

HALO image analysis platform Cytiva has partnered with Indica Labs, maker of the HALO image analysis platform, to offer the HALO platform as our Cell DIVE image analysis solution. The HALO image analysis platform is the gold standard for quantitative tissue analysis in digital pathology.

With ease of use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, pharmaceutical, healthcare, and research organizations worldwide are using the HALO imaging analysis platform. The platform delivers high-throughput, quantitative tissue analysis in oncology, neuroscience, metabolism, toxicology, and more.

The HALO modules that are most relevant to the Cell DIVE workflow are detailed below. This is not a comprehensive list of all available HALO analysis modules.

• Highplex FL: Quantify expression of an unlimited number of biomarkers in any cellular compartment (membrane, nucleus, or cytoplasm).

• Tissue Classifier: Separate multiple tissue classes across a tissue using a learn-by-example approach. This module can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

• Spatial Analysis: Plot cells and objects from one or more images and perform nearest neighbor, proximity, and tumor infiltration analyses.

Table 15. Recommended HALO workstation* and monitor specifications

Parameter Specification

Operating system

Windows 7 SP1 or laterWindows Server 2008 R2 or laterOnly 64 bit operating systems are supported

CPU Single or dual Intel Xeon 8 core plus Hyper-Threading2.0 GHz or fasterCPUs with more cores will enable higher analysis throughput

Memory 16 GB or moreA higher amount of memory will improve HALO performance

Storage SSD drives recommended1 TB local or network storage recommended for markup images500 GB+ local storage for MySQL database

Monitor 24-inch or more recommended1920 × 1080 resolution4K resolution currently not supported

* A separate workstation is needed for HALO analysis and is not provided as part of the Cell DIVE imaging solution. For optimal performance of HALO AI specifically, more robust specifications may be required.

AutomationThe Cell DIVE imager can be integrated with many commercially available automation/robotic systems to enable true unattended imaging.

Preferred vendor Peak Analysis and Automation (PAA)

Preferred models S-Lab™ automated plate handlerGX™ robot

Warranty informationThe Cell DIVE imager includes a one-year warranty that covers all base system components and optional accessories described in this document, subject to terms and conditions.

Ordering informationDescription Product code

Cell DIVE imager (includes acquisition workstation) 29429159

Cell DIVE license (1 year) 29262872

20× 0.75 NA CFI Plan Apo Lambda 28953478

Open well cassette ×1 29463259

Open well cassette ×10 29463260

Dual slide cassette ×1 29431398

Dual slide cassette ×10 29431399

PAA S-LAB plate handler 29376735

PAA GX robot 29376736

For local office contact information, visit cytiva.com/contact

Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate. Cell DIVE and Cy are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

HALO is a trademark of Indica Labs, Inc. Windows is a registered trademark of Microsoft Corporation. All other third-party trademarks are the property of their respective owner.

Cell DIVE Staining Methods. Methods relating to sequentially staining and probing samples to obtain multiplexed images is covered by US patent numbers 7,629,125; 9,164,015; 8,568,991; 9,176,032; 9,250,245; 9,677,125 and equivalent patents in other countries owned by Cytiva. The purchase of a CellDIVE License provides users with the rights to practice these patents under the terms of the license. For more information regarding the license and permitted uses contact lslicensing@cytiva.com Cell DIVE Software. Software for multiplexed imaging, including methods for acquisition, analysis and data visualization for multiplexed imaging is covered by US patent numbers 8,131,476; 8,880,351; 8,189,884; 8,135,187; 8,995,740; 8,639,013; 9,164,015; 8,873,827; 9,135,694; 9,607,375; 9,613,254; 9,778,263; 10,019,796; US Patent Appls 14/730032 and 14/730037and equivalent patents in other countries owned by Cytiva. The purchase of a CellDIVE License provides users with the rights to practice these patents under the terms of the license. For more information regarding the license and permitted uses contact lslicensing@cytiva.com The Cell DIVE imager is a Class 1 laser product.

All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

CY11898-09Apr20-DF

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