Please refer disclaimer Overleaf.

M1661Caulobacter Medium

Ingredients Gms / LitrePeptone 2.000Yeast extract 1.000Magnesium sulphate. heptahydrate 0.200Agar 10.000Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters

DirectionsSuspend 13.10 grams (theequivalent weight of dehydrated powder per litre) in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.

Principle And InterpretationCaulobacter is a gram-negative bacterium, which resembles the aerobic, chemoheterotrophic Pseudomonades, with which they often share their natural habitats. Caulobacter generally live in a dilute aquatic environment where the most common limiting factor is phosphorus, an essential element for healthy growth. Caulobacter belongs to the group of dimorphic prosthecate bacteria (DPB) where reproduction takes place in an asymmetric manner rather than by binary fission. The daughter cells produced are morphologically and behaviorally different from each other, which makes them a suitable model to study regulation of cell cycle and cellular differentiation. Lack of nutrients makes Caulobacter to dramatically elongate its stalk up to 30 times longer than those in phosphrous-rich medium (5). They are tolerant to prolonged nutrient scarcity, which provides a dependable physiological basis for their enrichment (3).Caulobacter Medium was developed by using the formula of Poindexter (9), by addition of solidifying agent, agar. It is recommended for cultivation of Caulobacter species (1). This medium is supplied as Medium 28 for Caulobacter by Pasteur Institute (4). This medium was also used by Qi and Bernd (10) to study polyhydroxybutyrate biosynthesis. The importance of employing dilute media was discovered during the first reported isolation of Caulobacter by Loeffler (8).Caulobacter Medium is low in nutrient concentration. Growth of Caulobacter in rich media or in severely unbalanced media is extremely poor if it occurs and the cells are structurally fragile and morphologically aberrant. This medium has peptone and yeast extract as ingredients, which act as source of nitrogen, amino acids and vitamins for the growth of organisms. Magnesium sulphate supplies essential ions for Caulobacter growth.

Composition**

Intended Use:Recommended for cultivation of Caulobacter species.

Type of specimen Water samples

For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2) After use, contaminated materials must be sterilized by autoclaving before discarding.

Specimen Collection and Handling:

Warning and Precautions :Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium

2-Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.

Technical Data

Organism Growth

Caulobacter crescentusATCC 15252

good-luxuriant

Caulobacter fusiformisATCC 15257

good-luxuriant

Reference

5. Gonin M., Quardoleus E. M., ODonnol D., Maddock J., and Brun Y. V.,2000, J. Bacteriol., 182:337

9. Poindexter J. S., 1964, Bacteriol. Rev., 28:23110.Qi Qingsheng and Bernd H. A. Rehm, 2001, Microbiology, 147:3353

Revision : 02 / 2020Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.0% Agar gel.Colour and Clarity of prepared mediumLight to medium amber coloured, clear to slightly opalescent gel forms in Petri platesReactionReaction of 1.30 w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseCultural characteristics observed after an incubation at 30-35°C for 4-7 days.

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3. Further biochemical and serological tests must be carried out for further identification.

expiry period Performance and EvaluationPerformance of the medium is expected when used as per the direction on the label within thewhen stored at recommended temperature.

Storage and Shelf LifeStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).

Disposal

2. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C.

3. Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H., (Eds.), The Prokaryotes, 1992, 2nd Edition, Vol.III, Springer-Verlag.

1. Atlas R. M., 2004, Handbook of Microbiological Media, 3rd Edition, CRC Press.

4. Collection Institute Pasteur Medium Description, Institute Pasteur.

6. Isenberg, H.D. Clinica Microbiology Procedures Handbook 2nd Edition.7. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual

of Clinical Microbiology, 11th Edition. Vol. 1.8. Loeffler F., 1980, Bakteriol. Parasitenkd., 7:625-639.

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