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DOI 10.13350 j.cjpb.180302 ·!"· )*+,- Ca ./0123$45678%9 :;<=>·:?@ABC :?@DE<·:?@ABC #FG HIJ ;KL·M<N OPQ· RS ;T=>·UVW XSYW·ZUW [$ 1. Þ+/%&ß¡¸&àà;Þáâãä 830011 2. uåæçè/&顸&àà;3. Þ+/%&'()*+,-./4. êëìíînB+,-./5. ïÏ2+&/&,ïÏ2ð¡ñàáD!"#$ _N¡¸óô&iA[õö÷=> CagA YZøùúûK9üuý%& ÿ NCBIãþÿ Y CagA YßKYZóô_N ProtParam SignaIP4.1 TMHMM iA[ CagA YZøùúûK´!"ó}#u $%J»Ný¶iúû®&9üÐ w úû '& 9 üd \'( CagA úû s 1172 t S Y U;? % p 5742915116151845 13 ¥n Alao7.3% Arg o9.9% Valo2.8% ¨u()YAsp+Glu 162t*u()YArg +Lys 167t;?"+ÂOp130747.31 ´!_uPp8.42 ,'³ã18.53 p,'úû-Þ½Ý`ãp0.752 å-â-Þ®&9ün α ÷.Hh 602to51.37% lmPEe 147to 12.54% β /0Tt 99to8.45% 12¹ó3Cc 324to27.65% ') ¡ ¸ óô &;ï õö÷ =>cagA YZ øùúû p , 'úû 4-â-Þåp¥·³àáu{5|O67Kà +,-.8p9:9;<=®³>;<Kò?+,ðK @=*+,õö÷=>CagA úû¡¸óô&-./01R378.91 23456278116735234 2018 03022105 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔.2018Mar 13 ): 221-225. 犅犻狅犻狀犳狅狉犿犪狋犻犮犪狀犪犾狔狊犻狊狅犳狋犺犲犆犪犵犃犵犲狀犲犻狀犎犲犾犻犮狅犫犪犮狋犲狉 狆狔 犾狅狉犻 AZIERGULI · abudoukerimu ABUDOUHABAER · abudoukerimu LIUYumei XUHaitao REBIYA · nuli ZILAIGULI · mijiti ZIBAIDAN · tuerxun DELIXIATI · yimiti YUTao 犇犲 犪狉狋犿犲狀狋狅 犕犻犮狉狅犫犻狅犾狅 犵狔 犡犻狀 犻犪狀 犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犠狌犾狌犿狌 830011 犆犺犻狀犪 犇犲 犪狉狋犿犲狀狋狅 犅犻狅犾狅 犵狔 犎狅狋犪狀犜犲犪犮犺犲狉狊犆狅犾犾犲 犇犲 犪狉狋犿犲狀狋狅 犆犾犻狀犻犮犪犾犔犪犫狅狉犪狋狅狉 犡犻狀 犻犪狀 犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犆犾犻狀犻犮犪犾犔犪犫狅狉犪狋狅狉 犆犪犾犪犿犪 犻犆犲狀狋犲狉犎狅狊 犻狋犪犾 犛犺犪狀犱狅狀 犐狀狊狋犻狋狌狋犲狅 犘犪狉犪狊犻狋犻犮犪犾犇犻狊犲犪狊犲犃犫狊狋狉犪犮狋 犗犫犼犲犮狋犻狏犲 TousebioinformaticsoftwaretopredictthestructureandfunctionoftheproteinthatCagAof 犎犲犾犻犮狅犫犪犮狋犲狉 狆狔 犾狅狉犻codesfor. 犕犲狋犺狅犱狊 BasicgeneticinformationonCagAwasobtainedfromtheNCBIdatabase.The softwareProtParam SignaIP4.1 andTMHMM wasusedtopredictthephysicochemicalproperties thesignalpeptide andthetransmembranedomainofthatprotein.Thesecondarystructureoftheprotein wasanalyzed andthetertiary structureoftheproteinthatCagAcodesforwas modeledusingExpasytoolsavailableonline. 犚犲狊狌犾狋狊 CagAprotein contains1172aminoacids.ItsmolecularformulaisC 5742915116151845 13 anditconsistsofAla 7.3% ), Arg 9.9% ), andVal 2.8% .Theproteinhas162negativelychargedresidues Asp+Glu and167positivelychargedresidues Arg +Lys .Itsmolecularweightwas130747.31 itsisoelectricpointwas8.42 anditsinstabilityindexwas18.53.The proteinisastableprotein itsaveragehydrophilicityindexwas-0.752 andthepredictedproteinisahydrophobicpro tein.Thesecondarystructureconsistedof602α helixes Hh ), accountingfor51.37%ofthestructure 99 β turns Tt ), accountingfor8.45% 147extendedstrands Ee ), accountingfor12.54% and324randomcoils Cc ), accountingfor 27.65%. 犆狅狀犮犾狌狊犻狅狀 ThesefindingsaresignificantforfurtherstudiesontheantigenicityofH.pyloricagAandthede velopmentofefficientepitopevaccines andthesefindingsprovideanewtargetforthetreatmentofgastriccancer gastric ulcers andduodenalulcers. 犓犲狔狑狅狉犱狊 犎犲犾犻犮狅犫犪犮狋犲狉 狆狔 犾狅狉犻 CagAprotein bioinformatics analysis õö÷ => 犎犲犾犻犮狅犫犪犮狋犲狉 狆狔 犾狅狉犻 Hp ( A å'BChð¡ý9>DnK-âî\¡¸&@P KKEFj GH ß Á I >ý J â 44 K Hp ij¢½Ýijcp 62.8% ¦§mÖ Hp ijc Jp 50% LM p 30% ijmã{ ¥ 7.68 KHp · · . /0123456 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔 2018ñò '13ó'h March2018 Vol.13 No.3 9:;#§qH/&YôõäNo.30860385 )。 <=>? NEmail yutao5816 126.com >?@AOPQRS·O¢TêUã1984- ), VÞ uåmûüWæàá}X>DÇ@K¼`Email arzu64 sina.com

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Page 1: CagA ./0123$45678%9

DOI:10.13350/j.cjpb.180302 ·!"

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[犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔.2018Mar;13(3):221-225.]

犅犻狅犻狀犳狅狉犿犪狋犻犮犪狀犪犾狔狊犻狊狅犳狋犺犲犆犪犵犃犵犲狀犲犻狀犎犲犾犻犮狅犫犪犮狋犲狉狆狔犾狅狉犻

AZIERGULI·abudoukerimu1,ABUDOUHABAER·abudoukerimu2,LIUYumei3,XUHaitao4,

REBIYA·nuli1,ZILAIGULI·mijiti1,ZIBAIDAN·tuerxun1,DELIXIATI·yimiti

1,YUTao5 (1.

犇犲狆犪狉狋犿犲狀狋狅犳犕犻犮狉狅犫犻狅犾狅犵狔,犡犻狀犼犻犪狀犵犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔,犠狌犾狌犿狌狇犻830011,犆犺犻狀犪;2.犇犲狆犪狉狋犿犲狀狋狅犳犅犻狅犾狅犵狔,

犎狅狋犪狀犜犲犪犮犺犲狉狊犆狅犾犾犲犵犲;3.犇犲狆犪狉狋犿犲狀狋狅犳犆犾犻狀犻犮犪犾犔犪犫狅狉犪狋狅狉狔,犡犻狀犼犻犪狀犵犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔;4.犆犾犻狀犻犮犪犾犔犪犫狅狉犪狋狅狉狔,

犆犪犾犪犿犪狔犻犆犲狀狋犲狉犎狅狊狆犻狋犪犾;5.犛犺犪狀犱狅狀犵犐狀狊狋犻狋狌狋犲狅犳犘犪狉犪狊犻狋犻犮犪犾犇犻狊犲犪狊犲.)

【犃犫狊狋狉犪犮狋】 犗犫犼犲犮狋犻狏犲 TousebioinformaticsoftwaretopredictthestructureandfunctionoftheproteinthatCagAof

犎犲犾犻犮狅犫犪犮狋犲狉狆狔犾狅狉犻codesfor. 犕犲狋犺狅犱狊 BasicgeneticinformationonCagAwasobtainedfromtheNCBIdatabase.The

softwareProtParam,SignaIP4.1,andTMHMMwasusedtopredictthephysicochemicalproperties,thesignalpeptide,

andthetransmembranedomainofthatprotein.Thesecondarystructureoftheproteinwasanalyzed,andthetertiary

structureoftheproteinthatCagAcodesforwasmodeledusingExpasytoolsavailableonline. 犚犲狊狌犾狋狊 CagAprotein

contains1172aminoacids.ItsmolecularformulaisC5742H9151N1615O1845S13,anditconsistsofAla(7.3%),Arg(9.9%),

andVal(2.8%).Theproteinhas162negativelychargedresidues(Asp+Glu)and167positivelychargedresidues(Arg

+Lys).Itsmolecularweightwas130747.31,itsisoelectricpointwas8.42,anditsinstabilityindexwas18.53.The

proteinisastableprotein,itsaveragehydrophilicityindexwas-0.752,andthepredictedproteinisahydrophobicpro

tein.Thesecondarystructureconsistedof602αhelixes(Hh),accountingfor51.37%ofthestructure,99βturns(Tt),

accountingfor8.45%,147extendedstrands(Ee),accountingfor12.54%and324randomcoils(Cc),accountingfor

27.65%. 犆狅狀犮犾狌狊犻狅狀 ThesefindingsaresignificantforfurtherstudiesontheantigenicityofH.pyloricagAandthede

velopmentofefficientepitopevaccines,andthesefindingsprovideanewtargetforthetreatmentofgastriccancer,gastric

ulcers,andduodenalulcers.

【犓犲狔狑狅狉犱狊】 犎犲犾犻犮狅犫犪犮狋犲狉狆狔犾狅狉犻;CagAprotein;bioinformatics;analysis

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March2018,Vol.13,No.3

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Ala 86 7.3

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Gly 68 5.8

His 13 1.1

Ile 61 5.2

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March2018,Vol.13,No.3