by the name of allah
DESCRIPTION
By the name of Allah. Report about: Bacterial Motility by : Islam Radi 220050911 Supervised by Dr. Abdelraouf A. Elmanama. introductio n. Most bacteria, but not all, that are motile, move by using structures call FLAGELLA - PowerPoint PPT PresentationTRANSCRIPT
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By the name of Allah
Report about:
Bacterial Motility by:
Islam Radi 220050911
Supervised byDr. Abdelraouf A. Elmanama
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introduction
Most bacteria, but not all, that are motile, move by using
structures call FLAGELLA
Flagella are long rigid rod-like structures made of repeating protein subunits
They are attached to a MOTOR located in the cell wall that turns them like a propeller.
The flagella motor is powered by a flow of PROTONS, sort acting like electricity
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•As flagella rotate they turn or rotate like a propeller and drive the bacterial cell through liquid
•We will use this ability to detect whether the test bacteria are motile or non-motile
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The following flagellar arrangements may be found
Monotrichous: a single flagellum at one pole
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amphitrichous : single flagella at both poles
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Lophotrichous: two or more flagella at one or both poles of the cell
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peritrichous : completely surrounded by flagella
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MOTILITY AGAR
** Motile bacteria require liquid to move .
Thus bacteria can propel themselves in broth or across the surface of a
wet agar plate.
**They will not however move when embedded in 1.5% agar, the
minimum concentration found in most agar media .
**Semisolid agar has a reduced agar concentration (0.4 %) that allows
flagellated bacteria to migrate from the site of inoculation..
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** Semisolid media are prepared in tubes and are inoculated through most of their length by stabbing with a needle.
** Thus after 48 hours of incubation, growth of a motile organism will be observed as a turbid region extending from the stab.
** Nonmotile bacteria will only grow along the stab line
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** inoculate the tube by stabbing with the needle to approximately three-quarters of its depth
Be careful to bring the needle into the center of
the medium and not to touch the side of the tube
•Incubate at room temperature for 48 hours
•** Examine for growth
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Motile
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•Non motile
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HANGING DROP SLIDE
**This method is commonly used to view living organisms for the rapid determination of motility.
** The hanging drop is prepared by suspending a fluid sample from a coverslip over a depression well in a specially designed microscope slide.
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MATERIALS
*Depression slides
Cover slips **
***Petroleum jelly
****Toothpicks
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Procedure
* With the toothpick , put a small amount of petroleum on
each corner of the cover slip
**place one or tow loopful of a culture in the center of
cover slip
***the depression slide is placed on the cover slip with the
depression over the frop of fluid ****Examine with low power
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