bioprocessing media and buffers product catalog
TRANSCRIPT
Bioprocessing Media and BuffersProduct Catalog
– For cell line development and media selection k Standard off-the-shelf media High quality | high performance serum-free formulation for all cell lines in bottles and small bags
– For media optimization k Customized media optimization service and support R&D specialists can optimize or fully develop new custom media engineered to your unique cell culture specifications
– For process development k Fast and clean custom production An expedited production option for those that require high quality cell culture media for process optimization or process development as well as quick buffer delivery
– For production k Custom media production under cGMP Protocols Small to large batch production following Current Good Manu-facturing Practices (cGMP 21CFR820) in ISO certified facilities Manufacturing practices compliant with standard regulations
Complete Solution
Media | Buffer Preparation Upstream Process
Pre-Viral Downstream Process
Post-Viral Downstream Process
LevMixer
Protein A Chromatography
FlexAct®
Biostat® B, RM, UniVesselFlexAct®
Low-pH Virus Inactivation
UF | DF
Sterile Filtration
CEX Chromatography
Storage
Storage
Storage
Wash | Equilibration | Elution buffers
Media
pH Adjustments buffers
Media and Feeds
Flush | Equilibration buffers
Dilution | Stabilization buffers
Processing Path Showing USP | DSP Elements
Wash | Equilibration | Elution buffers
Pre-Clinical
Wash | Flush | tize | Diafiltration buffers
A Media Solution to Fit Your Cell Line, Schedule and Budget
Features Benefits
Media and buffers in Sartorius Stedim bags kSame material for single use bioreactor bags to minimize the risk of leachables
Full media qualification package and support. All liquid media filtered through Sartorius filters
kOptimized media filtration and guaranteed successful scale-up thanks to complete support provided
Preweighed powder buffer and media in dispensing bags k Simple, ready-to-use media and buffers fully compatible with mixing technology
Integrated media feed strategies and PAT automation k Fully monitored and controlled cell culture conditionsProducts fully integrated into the Sartorius upstream and downstream solutions
kOne-stop supplier gives you full support so you can focus on your core tasks
Complete Solution
All the equipment and solutions you may need for your media and buffer preparation steps can also be provided by Sartorius, please refer to Section Related Products page 100-108.
Sterile Filtration
Biostat STR®
Polishing
FlexAct®
Storage
Depth Filtration
FlexAct®
Polishing
Virus Filtration
Storage
Storage
Filling Freezing
Media and Feeds
Flush | Equilibration buffers
Dilution | Stabilization buffers
Buffer Flush buffers
Cell Growth Media Buffers
Wash buffers Wash | Equilibration | Elution buffers
Glossary for Pictograms | Product FeaturesThe following pictograms indicate the available features of our products presented in a format that allows you to easily identify the information that matters to you most.
Animal Origin: formulation contains animal or human origin components
Non Animal Origin: the formulation is entirely made from non-animal and non-human origin components
Chemically Defined: The exactconcentration and size of everycomponent is known
For Further Manufacturing Use
Research Use Only
Product available in liquid format
Product available in powder format
Protein-free*: the formulation does not contain any animal-derived proteins
As part of our high manufacturing standards, all the products we supply in liquid format have been sterile filtered through Sartorius and made according to cGMP (21CFR820) conditions at ISO facilities.
* human recombinant insulin is NOT considered a protein, both because it is fully characterized, and because of its size (<5kDa) per Lonza definition this means that it does not include any proteins/peptides >10 kDa.
Bioprocessing Media and Buffers
Table of Contents
Upstream ApplicationsMulti Purpose Media p.14
Ultra Culture™ p.14
Insect-Xpress™ p.15
Recombinant Proteins p.17
PowerCHO™ Protein Expression Media p.18
PowerCHO™ Advance™ p.20
ProCHO™ p.22
Feeds p.23
GS-CHO p.24
CHO Media – The Optimization Tool Box p.26
CHOptimizer – The Media Builder Box p.28
ProNSO™ p.30
UltraDoma™ p.32
UltraDOMA-PF™ p.33
ProDoma™ p.34
Viral Vaccines p.35
ProMDCK™ p.36
UltraMDCK™ p.37
ProVero™ 1 p.38
ProPer™ 1 p.39
PERMEXCIS® p.40
Pro293™ p.42
Regenerative Medicine p.45
X-Vivo™ p.46
L7™ hPSC Culture System p.48
FGM-CD™ (Fibroblast Growth Media) p.50
KGM-CD™ p.51
MSCGM-CD™ p.52
Bioprocessing Media and Buffers
Downstream StepsThe Downstream Buffer Offering p.54
Buffer Packaging and Options p.56
DSP Buffers p.57
PBS p.58
DPBS p.59
Hanks p.60
Hepes p.60
Earle’s p.61
Other Buffers p.61
WFI p.62
ReagentsSodium bicarbonate p.64
Sodium pyruvate p.64
Insulin p.65
Trypsin p.65
L-Glutamine p.66
Ultra Glutamine p.66
Penicillin p.67
Amphotericin p.68
Gentamycin p.68
ProFreeze™ p.69
Anticlumping Agents p.70
ServicesCustomization p.74
Support p.74
Validation p.74
Quality p.76
Packaging and Shipping p.80
Introduction p.8
Flexibility p.10
Custom Production p.11
Table of Contents
Related ProductsSolutions for Media Preparation p.100
Sartopore® 2 XL Filter Family p.102
Sartogard PreFilter Familyy p.103
Virosart® p.104
UVivatec® p.105
Flexel® for Mixing1 p.106
FlexAct® MP p.107
Which Bioreactor Fits your Needs? p.108
Classical MediaBME (Basal Medium Eagle) p.84
DMEM (Dulbecco’s Modified Eagle’s Medium) p.84
EMEM (Eagle Minimum Essential Medium) p.86
DMEM:F12 Medium p.88
GMEM (Glasgow Minimum Essential Medium) p.88
Grace’s Insect p.89
Ham’s p.89
IMDM (Iscove’s Modified Dulbecco’s Medium) p.90
L-15 (Leibovitz) p.90
Mc Coy’s p.91
M199 p.91
PC-1™ p.92
HL-1™ p.94
RPMI p.96
NCTC-109 p.97
William’s E p.97
Richter’s p.97
UltraMEM™ p.98
8
In January 2013, Sartorius Stedim Biotech and Lonza joined forces to supply high quality, innovative cell culture media and buffers as well as complementary single-use solutions for biopharmaceutical, vaccine and cell therapy development and production.
Lonza-Sartorius offers a wide range of liquid and powder media products taking advantage of their respective expertise to help you scale-up your project and optimize your processes thanks to our leading position in single-use applications such as fluid management, filtration and process analytical technology.
Lonza-Sartorius is a pioneer in state-of-the-art micronization and high-quality powder production and is ISO-certified with a successful audit track record following current Good Manufacturing Practices 21CFR820.
Introduction
Florenz Sartorius mechanical workshop is founded
Sartorius starts commercial-scale manufacturing of membrane filters
Sartorius is founded as the original manu- facturer of EVA plastic bags for total parental nutrition
1870 1922 1990 19941948 1970 1974 1979
Founded Media Production starts
Grand opening of the Walkersville facility in Maryland, USA
Initial manu- facture of liquid media in Verviers, Belgium, EU
Stedim creates the market for bag systems used in biopharmaceutical manufacturing processes
9
Introduction
1999 2000 20142007 2008
Acquisition of Cambrex Bioscience division by Lonza
Sartorius acquires B. Braun Biotech
Sartorius Stedim Biotech is established by the reverse merger of Sartorius AG‘s Biotech division into Stedim
Sartorius acquires Wave Biotech
Sartorius acquires
Sartorius acquires Vivascience
Complete offering with complementary product lines to address all your bioprocessing needs
Long-term Collaboration
2013 2015
Sartorius acquires UK-based CRO, BioOutsource
50 years of culture media experience backed by 150 years of pharma industry innovations have led to long-term relationships with dual sourced raw material suppliers which guarantees supply and quality as well as excellent regulatory support for all your projects.
The Cell Culture Media division of Sartorius is pleased to present to you this catalogue which displays the wide variety of products we offer.
Sartorius acquires Cellca – a cell line development company
10
Flexibility
Verviers, Belgium
Sartorius helps its customers develop and manufacture biotech medicines reliably, quickly, and economically. Sartorius operates as a global player in dynamic markets around the world. Yet the group has retained much of its home-grown identity – strong regional ties and a personal corporate culture that has remained strongly intact since the company’s origins as a family business.
Today, more than 6,000 people work together at Sartorius worldwide. They hail from a diversity of cultures with different traditions, work styles and languages. What unites all employees at Sartorius is their strong identification with the company and its common vision, mutual goals and strategies, as well as a common set of values they all share: Sustainability, Openness and Enjoyment.
The Sartorius Bioprocess Solutions Division is a leading international provider of integrated solutions for the biopharmaceutical industry. With our extensive portfolio of products, technologies and services, we cover wide areas of the biopharmaceutical process chain, ranging from fermentation, cell cultivation, filtration and
purification to media storage and transportation. In process filtration, fermentation and fluid management, our company holds leading positions in the markets it serves across the globe. Our single-use products that have meanwhile become established in nearly all process steps are playing an increasingly important role in the manufacture of biopharmaceuticals. As a result, we help our customers produce biological medications and vaccines safely and efficiently.
k Assurance of supply – dual production sitesk Batch size scalability – liquid media: 1 L to 10,000 L k Powder media: 7 kg to 7,000 kg k Wide range of primary and secondary packaging
to meet specific needs k Off-the-shelf and fully customized solutions
and formulations k Media formulation options include animal origin
(AO) and non-animal origin (NAO), serum-free, protein-free and chemically defined with full traceability to provide solutions for any type of process
k Media formulation options for a broad range of cells such as hybridoma, CHO, insect cells, kidney cells, primary cells, stem cells and microorganisms
Features
11
In addition to off-the-shelf media and buffers, Sartorius also offers tailor-made production of Lonzas' proprietary formulations or of our customers’s own formulations. We offer a wide array of liquid and powder solutions along with the expertise to help you develop your project from clinical trials to commercialization. Offered
Dedicated support from both Sartorius and Lonza teams are assigned for each custom manufacturing project. Each project is treated with high confidentiality and matches customer requirements.Specific documentation is available to help our customers build a regulatory dossier.* up to 4000 L in small bags or bottles for AO and NAO media and up to 10000 L in big bags (> 50 L size)
Please contact your local representative for more detailed information on how Sartorius can support your project.
Fully Traceable Raw Materials for Media and Disposable System Scalability from R&D to Commercialization
Process Development
Scale-Up | Pilot Plant
CTM (Clinical Trial)Launch Facility
Commercial Manufacturing
Walkersville, MD, USA
Walkersville MD, USA Verviers, Belgium
cGMP (21CFR820) based manufacturing facility ✓ ✓
ISO 9001:2008 certified ✓ ✓
ISO 13485:2008 certified ✓ ✓
Liquid media capabilities (per batch) Up to 10,000 L Up to 10,000 L*
Powder media capabilities (per batch) NAO Medium
Up to 700,000 L Up to 100,000 L
Batch capacity 7-7290 Kg 20-1200 Kg
products and services include specially engineered media and buffers for dedicated USP | DSP | F&F unit operations. Taking benefit of the 2 manufacturing sites of Lonza in Belgium (Verviers) and USA (Walkersville), we offer a capacity of liquid production up to 10,000 L per batch and up to 7 tons of powder.
Custom Production
Cell LineDevelopment
12
A broad range of off-the-shelf media are available in liquid and powder format to provide you with maximum flexibility during early development.We offer special kits for cell line and media development as liquid (1 – 20 L) and as powder versions (up to 5 kg).
Our Range of Cell Culture Media
Cells Medium Culture Primary application
CHO (e.g., DG44, CHO-S, CHO K1, DHFR–)
UltraCHO Suspension and Adherent Proteins
ProCHO®-5 Suspension Proteins
PowerCHO™-2 Suspension Proteins
PowerCHO™-3 Suspension Proteins
ProCHO®-AT Adherent Proteins
PowerCHO™ Advance Suspension Proteins
CHOK1SV (GS platform) PowerCHO™-GS Suspension Proteins
Lonza GS V8 Media and feeds* Suspension Proteins
Hybridoma HL-1 Suspension Proteins
ProDoma-3 Suspension Proteins
UltraDoma Suspension Proteins
UltraDoma PF Suspension Proteins
NS0 ProNS0 Suspension Proteins
Insect cells (e.g., Sf9, Hi 5) Insect Xpress Suspension Proteins | Vaccines
Vero ProVero-1 Adherent Vaccines
PC-1 Adherent Vaccines
ProMDCK Suspension and Adherent Vaccines
MDCK UltraMDCK Adherent Vaccines
ProMDCK Suspension and Adherent Vaccines
HEK 293 Pro293a Adherent Vaccines
Pro293s Suspension Vaccines
PerC6* Permexcis* Suspension Vaccines
PerC6 (and retinoblastoma cell lines) ProPER1 Suspension Vaccines
Hematopoietic cells (e.g., T, NK, DC)
X-Vivo 10 Suspension Cell therapy
X-Vivo 15 Suspension Cell therapy
X-Vivo 20 Suspension Cell therapy
HL-1 Suspension Cell therapy
Mesenchymal stem cells MSCGM CD Adherent Cell therapy
Adult dermal fibroblasts FGM CD Adherent Cell therapy
Adult and neonatal primary keratinocytes
KGM CD Adherent Cell therapy
NAO: Non-animal originCD: Chemically defined* License required
Upstream Applications
Sartorius can offer specialty media dedicated to vaccines and cell therapy applications depending on the cell lines used. Sartorius offers mainly serum-free media and some of them are non-animal origin (NAO) certified to ensure maximal safety for customers whose goal is to develop cell cultivation processes dedicated to dedicated to protein manufacturing, vaccines and regenerative medicine applications…
Upstream Processing
USP
Multi Purpose Media p.14
Ultra Culture™ p.14
Insect-Xpress™ p.15
Recombinant Proteins p.17
PowerCHO™ Protein Expression Media
p.18
PowerCHO™ Advance™ p.20
ProCHO™ p.22
Feeds p.23
GS-CHO p.24
CHO Media - The Optimization Tool Box
p.26
CHOptimizer: The Media Builder Box
p.28
ProNSO™ p.30
UltraDoma™ p.32
UltraDOMA-PF™ p.33
ProDoma™ p.34
Viral Vaccines p.35
ProMDCK™ p.36
UltraMDCK™ p.37
ProVero™ 1 p.38
ProPer™ 1 p.39
PERMEXCIS® p.40
Pro293™ p.42
Regenerative Medicine
p.45
X-Vivo™ p.46
L7™ hPSC Culture System p.48
FGM-CD™ Fibroblast Growth Media
p.50
KGM-CD™ p.51
MSCGM-CD™ p.52
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Ordering Information
UltraCULTURE™
UltraCULTURE™ Medium is a serum-free medium designed for the cultivation of a wide variety of mammalian cell types. The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/mL.
UltraCULTURE™ Medium does not contain L-glutamine. UltraCULTURE™ Medium has been used to grow a variety of cells of primary origin and established cell lines. The medium may be used to support the fusion of cells during the formation of hybridomas.
UltraCULTURE™ Medium has been used to grow cells of lymphoid origin including monocyte and macrophage cell lines. Epithelial and fibroblastic cells have been cultivated in UltraCULTURE™ Medium and endothelial cells have been cultivated under reduced serum concentration (5% fetal bovine serum using UltraCULTURE™ Medium as a base formulation).
UltraCULTURE™ Medium has also been used as a complete medium to support the generation of viral particles for use in vaccine production. UltraCULTURE™ Medium may be supplemented with cryoprotective medium to cryopreserve cells in a serum-free environment.
EB-3 Human Burkitt’s lymphoma
K-562 Human Chronic myelocytic leukemia
HNK Human Neonatal kidney (primary)
HTC29 Human Colon Cancer
TT Human Medulary thyroid tumor
MB231 Human Breast carcinoma
U138 Human Glioma
FM3A Mouse Breast cancer
NS-1 Mouse Myeloma
Cell line Source Cell type
HEL, N-10 Human Fetal lung diploid fibroblast
HeLa Human Uterine cancer
HuL-1,2 Human Liver (normal)
HuK-1 Human Kidney (normal)
HuS-1AT Human Skin
HEC Human Embryonic cancer
HL-60 Human Acute promyelocytic leukemia
Raji Human Burkitt’s lymphoma
Partial List of Cells Cultivated with UltraCULTURE™ Medium:
L Mouse Subcutaneous
P388D1 Mouse Macrophage-like
P815 Mouse Mast cell tumor
T3 Mouse Pituitary
B82 Mouse L cells –connective tissue
RPL-1 Rat Peritoneum
RSP-2 Rat Spleen
RLG-1 Rat Lung
Lym-1 Rat Lymph node
RCR-1 Rat Brain
235-1,MNQ Rat Pituitary
GC, GH3 Rat Pituitary
CA77 Rat Medulary thyroid tumor
Rat-1 Rat Fibroblast
JTC-12 Monkey Kidney
COS1, COS7 African green
monkey
SV40 transformed kidney
Features of the standard product
Storage 2ºC-8ºC
Upstream Applications
Cat. No. NA Cat. No. EU Product Description Size
BP12-725F BEBP12-725F UltraCULTURE serum-free w/o L-Gln 500 mL 500 mL
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Multi Purpose Media
Related Products
Ordering Information
Insect-XPRESS™ Medium was developed to support the growth of invertebrate cell lines, derived from the fall army worm, Spodoptera frugiperda mainly Sf9 and Sf21.The Insect-XPRESS™ Medium formulation is protein-free (all components are less than or equal to 10,000 mwt.) and has been designed to support the growth of insect cells under attachment-dependent or attachment-independent conditions.
Cell densities in excess of 8.3 + 106 cells/mL have been achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. Insect-XPRESS™ Medium may also be used for stationary monolayer cultures and shakeflask cultures. The Insect-XPRESS™ 1X liquid formulation contains L-glutamine and supports the production of recombinant proteins by cells infected with viral vectors such as the Baculovirus. Insect-XPRESS™ Medium may be used as a base for the cryopreservation of insect cells. We recommend that the Insect-XPRESS™ Medium liquid be mixed 50:50 with Cryoprotective Medium (Cat. No. 12-132) prior to cryopreservation.
Applicationsk Baculovirus propagationk Recombinant protein expression
Insect-XPRESS™
Product Name Page
ProFreeze™CD(2X) FreezeMedium – Chemically Defined 69
Grace‘s Insect Medium 89
Storage 2ºC-8ºC
Features of the standard product
Cat. No NA Cat. No EU Product Description Size
BE12-730P10 BE12-730P10 STD Insect-XPRESS w/L-Gln, 10 L bag 10 L
BP12-730Q BEBP12-730Q Insect-XPRESS w/L-Gln 1 L 1 L
BP12-730F BEBP12-730F Insect-XPRESS w/L-Gln, 500 mL 500 mL
16
Recombinant ProteinsRecombinant proteins are produced after insertion of the gene of interest into a suitable host cell genome. Cell culture media is needed in all upstream processes with animal cells as expression hosts. Choice of media depends on the cell line used, which itself is selected depending on the product of interest. In the early days of cell cultivation, the easiest way to promote cell growth was adding serum. Still today, in many lab applications, serum is a multipurpose supplement.
Serum is a natural product, which varies in composition and bears risk of infectious reagents, which is why serum-free media (SFM) are preferred. New generations of SFM contain no components of animal origin (classified as NAO). Downstream processing is simplified due to the medias' reduced protein content.
We offer a full range of products such as standard or customized cell culture media with various features, such as serum-free, protein-free, chemically defined and non-animal origin.Although the media is important, we also consider the entire process to bring you a complete and fit-for- purpose solutions through our fully complementary systems across the entire Sartorius portfolio.
Recombinant Proteins
PowerCHO™ Protein Expression Media
p.18
PowerCHO™ Advance™ p.20
ProCHO™ p.22
Feeds p.23
GS-CHO p.24
CHO Media – The Optimization Tool Box
p.26
CHOptimizer – MBB: The Media Builder Box
p.28
ProNSO™ p.30
UltraDoma™ p.32
UltraDOMA-PF™ p.33
ProDoma™ p.34
18
PowerCHO™ Chemically Defined, Serum-free CHO Media are the next generation in CHO media, optimized for cell growth and protein production. The PowerCHO™ Series are hydrolysate-free, serum-free and non-animal origin media for supporting high density CHO cells in suspension. For therapeutic bioprocessing applications, these protein-free formulations also facilitate both down-stream purification and regulatory compliance.
PowerCHO™ Protein Expression Media
Our years of extensive experience in providing serum-free CHO media to research and industrial customers provides the basis for making great media even better. Completely chemically defined and serum-free, these media outperform with multiple cell lines.
PowerCHO™ Media deliver many unique benefits including consistent results, simplified downstream purification, and maximized yield.
Whether or not you choose serum-free media for the convenience and consistency in your research applications, or if your work leads to downstream therapeutic or biopharmaceutical applications, PowerCHO™ Media offer the best choice for initiating your studies. To learn more about PowerCHO™ Media and other Specialty Media products, contact Sartorius.
Features of the standard product
Upstream Applications
Storage 2ºC-8ºC
19
Ordering Information
Recombinant Proteins
Cat. No. NA Cat. No. EU Product Description Size
12-771P10 BE12-771P10 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 10 L 10 L
12-771P20 BE12-771P20 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 20 L 20 L
15-771ND BE15-771ND STD PowerCHO™2 Powder kit 10 L 10 L
15-771NF BE15-771NF STD PowerCHO™2 Powder kit 50 L 50 L
15-771NJ BE15-771NJ STD PowerCHO™2 Powder kit 100 L 100 L
BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L
N/A BE15-770F STD PowerCHO™1 Powder kit 50 L 50 L
N/A BE15-771L STD PowerCHO™2 Powder kit 500 L 500 L
N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L
BP12-770Q BP12-770Q PowerCHO™-1 serum-free medium 1 L 1 L
BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1 L bottle 1 L
N/A BEBP02-042Q PowerCHO™-2 CD, w/o glucose, 1 L 1 L
BP12-771Q BEBP12-771Q PowerCHO™-2 CD, w/o L-Gln w/o PR or HT,1 L 1 L
BP12-772Q BEBP12-772Q PowerCHO™-3 CD, w/o L-Gln w/o PR or HT,1 L 1 L
Related Products
See list of feeds p.23.
20
The new PowerCHO™ Advance™ allows superior performance when compared to leading CHO media in the market. PowerCHO™ Advance™ provides optimal conditions in shake flasks as well as bioreactors to allow flexibility to all customers working with CHO cells. This new and improved version of PowerCHO™ allows for greater filterability and easy scale up with a regulatory friendly product.
Storagek 2°C to 8°C in a dark location is recommended before and
after L-glutamine supplementationk When stored in proper conditions this media can be used for
12 months from the date of manufacture
Upstream Applications
Key featuresk Chemically defined, no raw materials of animal origin,
serum-free and manufactured to regulatory standardsk Designed for growing and feeding CHO cells in serum-free
conditionsk Designed to work together as a complete system which allows
for easy scale upk PowerCHO™ Advance™ as a basal formulation and PowerFeed™
Advance™ as a feed for fed-batch culturesk Allows for easier filtration while maintaining cell growth and
viabilityk Provides protein titers equivalent or better compared to
competitorsk Labeled “For Further Manufacturing Use”k For cell culture use only and not approved for human or
veterinary use and neither for application in humans or animals, or for use in vitro diagnostic or clinical procedures
Advance Compared to Competitors
Storage 2ºC-8ºCPowerCHO™ Advance™
Features of the standard product
0%
20%
40%
60%
80%
100%
120%
0
50
100
150
200
250
300
350
400
PC Adv-PF Adv
A1-a1 A1-a2 A2-a1 A2-a2 B1-b1 B2-b1 C1-c1 D1-d1 D1-d2
Tota
l IgG
1
Tota
l cel
l (x
106 )
PowerCHO™ Advance with Feed Compared to Competitors in Shake Flasks - DP12 CHO
Day5 - total cells Day6 - total cells Day7 - total cells Day8 - total cells
Day9 - total cells Day10 - total cells Day12 - total cells
21
Recombinant Proteins
Upcoming new product:For more information about its availability please contact your local MPM or email [email protected]
Cat. No. NA Cat. No. EU Product Name Size Options on Request
12-927Q 12-927Q STD PowerCHO™ Advance™ 1 L
Ordering Information
Related Products
See list of feeds p.23.
22
Upstream Applications
The following media systems are available:
k ProCHO™ 4 Medium – For concurrent transition of adherent CHO cells to serum-free and suspension culture; supports faster doubling times. Cat. No. 12-029Q contains phenol red and 0.1% Pluronic® F-68. Does not contain L-glutamine, hypoxanthine, or thymidine. Cat. No. 04-919Q is similar to 12-029Q except that it does not contain phenol red.
k ProCHO™ 5 Medium – For CHO cells already growing in suspension; supports increased protein production. Contains 0.1% Pluronic® F-68. Does not contain L-glutamine, phenol red, hypoxanthine, or thymidine.
k ProCHO™ AT Medium – For adherent culture of CHO cells. Contains L-glutamine. Does not contain hypoxanthine or thymidine.
ProCHO™Non-animal Origin CHO Expression Media
Ordering Information
Features of the standard productProCHO™ Protein-free CHO Media were developed speci fically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These protein-free formulations support high-density cultures without the need for animal-derived components. Very low levels of recombinant insulin facilitate both downstream puri cation and regulatory compliance.
Storage 2ºC-8ºC
Cat. No. NA Cat. No. EU Product Description Size
15-766D BE15-766D STD ProCHO™ 5 Powder kit w/o PR 10 L 10 L
15-766F BE15-766F STD ProCHO™ 5 Powder kit w/o PR 50 L 50 L
15-766L BE15-766L STD ProCHO™ 5 Powder kit w/o PR 500 L 500 L
BE12-766P10 BE12-766P10 STD ProCHO™ 5w/Pluronicw/oL-Gln, HT,10 L 10 L
BE12-766P20 BE12-766P20 STD ProCHO™ 5w/Pluronicw/o L-Gln, HT,20 L 20 L
BP04-919Q BP04-919Q ProCHO™ 4 w/o Phenol Red, 1 L 1 L
BP12-029Q BEBP12-029Q ProCHO™ 4 w/o L-Gln, HT 1 L 1 L
N/A BEBP02-041Q ProCHO™ 5 w/o glucose, 1 L 1 L
BP12-766Q BEBP12-766Q ProCHO™ 5 w/Pluronic w/o L-Gln or HT, 1 L 1 L
BEBP02-016Q BEBP02-016Q ProCHO™ AT w/L-Gln 1 L, w/o HT 1 L
Related Products
See list of feeds p.23.
23
Product Lipids Glucose Trace Elements Amino Acids Vitamins Hydrolysates Proteins
ProCHO4 h h h h h h -
ProCHO5 h h h h h h -
PowerCHO™-1 h h h h h - -
PowerCHO™-2 h h h h h - -
PowerCHO™-3 h h h h h - -
PowerCHO™ Advance™
h h h h h - -
CHOptimizer* h h h h h - -
Which of our CHO Media better fits your requirements?
Recombinant Proteins
* Learn more about the CHOptimizer on page 26.
“ PowerFeed™ A and Xtreme™ feeds are formulated to maximize cell growth using Lonza CHO cell culture media ProCHO™ 4, -5 and PowerCHO™ 2.
PowerFeed™ A and Xtreme™ feeds are Chemically Defined and Protein-free feeds. Both products are formulated using all Non Animal Origin components.”
Feeds
Cat. No. NA Cat. No. EU Product Description Size
02-044Q BE02-044Q STD PowerFeed A, 1L bottle 1 L
15-044D BE15-044D STD PowerFeed A Powder kit 10 L 10 L
15-044F BE15-044F STD PowerFeed A Powder kit 50 L 50 L
15-044J BE15-044J STD PowerFeed A Powder kit 100 L 100 L
15-044Q BE15-044Q STD PowerFeed A Powder kit 200 L 200 L
BE02-056Q BE02-056Q STD CHO Xtreme Feed™ CD - 1 L bottle 1 L
24
Upstream Applications
Lonza’s powerful proprietary GS System™ uses a robust viral promoter and selection via glutamine metabolism to provide extremely rapid development of high-yielding, stable, cGMP-compatible mammalian cell lines.
The GS System™ is successfully used by over 85 global pharmaceutical and biotechnology companies and is familiar to all worldwide regulatory authorities.
The international use of the GS System™ by both commercial organizations and academic institutions has led to a large body of published information. New users can draw upon this material to expand their knowledge and skill base and improve their use of the GS System™.
GS-CHO™Version 8 (v8) Commercial Platform ProcessLatest version of the cell culture media and feed platform for the GS Gene Expression System™. This new offering includes Lonza’s proprietary chemically defined, animal component-free (CDACF) Version 8 (v8) Media and Feeds Platform which was designed in conjunction with the GS-CHO cell line.
The new v8 Platform incorporates GS-CHO specific CDACF cell culture medium, feed base powders and supplements and is globally manufactured and supplied by our custom media production facilities. The CDACF v8 Platform has been shown to reach yields up to 9.6 g/L and has also been used successfully with DHFR-CHO cell lines. This media is free from both royalty and license fees and is available to all holders of the GS Gene Expression System™.
The Version 8 AdvantageChemically defined and animal component-free (CDACF)Developed by Lonza specifically for GS-CHO cell linesNo royalties or license fees addedIncreased antibody yieldImproved cellular metabolism
Features of the standard product
25
Recombinant Proteins
Cat. No. NA Cat. No. EU Product Description Size
BE02-043E BE02-043E STD FeCitrate1000x PowerCHO™-GS kit,100 mL 100 mL
BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L
N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L
BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1 L bottle 1 L
BESP904 BESP904 Lonza medium base 1 20,18 g
BESP905 BESP905 Feed Base K 10,23 g
BESP906 BESP906 Feed Base L 28,7g
BESP907 BESP907 Feed Base M 2,36 g
BESP908 BESP908 Feed Base N 0,18 g
BESP799 BESP799 Feed base F powder 1,75 g
BESP862 BESP862 Feed base E powder 1,72 g
BESP808 BESP808 CHO Vitamin Mix 0,18 g
BESP794 BESP794 Feed Base A 43,06 g
BESP795 BESP795 Feed Base B 112,2 g
BESP734 BESP734 NMB1 22,04 g
BESP735 BESP735 NMB2 18,34 g
BESP1023 BESP1023 Feed Base R 114,52 g
BESP876 BESP876 Feed Base 2 6,47g
BESP887 BESP887 MEM Vitamin Powder 9,31 g
BESP899 BESP899 Feed Base G 42,63 g
BESP900 BESP900 Feed Base H 69,04 g
BESP901 BESP901 Feed Base J 133,87 g
BESP1021 BESP1021 LMdB2 19,97 g
BESP1022 BESP1022 Feed Base P 72,17 g
BESP797 BESP797 Feed Base D 51,88 g
BESP531E BESP531E SPE 100 mL
BESP531F BESP531F SPE 500 mL
BESP531Q BESP531Q SPE 1000 mL
BESP909E BESP909E LTEM ONE 100 mL
BESP909Q BESP909Q LTEM ONE 1000 mL
BESP910E BESP910E LTEM TWO 100 mL
BESP910Q BESP910Q LTEM TWO 1000 mL
BESP969E50 BESP969E50 SP6 50 mL
BESP969F BESP969F SP6 500 mL
BESP970E50 BESP970E50 SP7 50 mL
BESP970F BESP970F SP7 500 mL
BESP971E20 BESP971E20 SP8 20 mL
BESP972E50 BESP972E50 SP9 50 mL
BESP972F BESP972F SP9 500 mL
BESP807E BESP807E CHO Trace Element Stock Solution 100 mL
BESP807Q BESP807Q CHO Trace Element Stock Solution 500 mL
Ordering Information
CHO Media – The Optimization Tool Box
26
Benchmarking KitStandard Media Testing
Standard solutionsFast and EasyNo Optimization
CHOptimizer
The Media Builder BoxFast Optimization processFinal Formulation under controlFit-for-purposeProcess Optimization
Do It Yourself
State of the art multi-bioreactor (ambr) linked to DoE software under AS support
Fully outsourced process bringing you a turnkey solution
Upstream Applications
The biopharmaceutical industry uses mainly animal cell cultures for the production of proteins targeting human and animal diseases, the most commonly used cell line being CHO. These processes are highly challenging due to multiple factors, such as:
– Regulatory authorities, such as the FDA and the EMA, that supervise production processes and products to ensure the safety of commercial products
– Variability of cells and proteins that are produced– Variability of culture conditions and parametersIt is impossible to have one fit-for-all media, as cell lines all have their specificities and all need different feeding routines to outperform.
The best approach for you depends on several parameters such as your timeline, your target market, your cells, your product lifecycle, etc. We strongly believe in a systemic approach, giving you the flexibility to navigate between standard media to fully optimized custom media.
27
Media OptimizationService Project
Start from “scratch”Full serviceNeed to ship your cellsFully CustomizedOutsource and save
Recombinant Proteins
28
CHOptimizerThe Media Builder BoxBiopharmaceutical industry uses mainly animal cell cultures for the production of proteins targeting human and animal diseases. New development processes are complex and highly challenging due to multiple factors as:
k Variability of the cell lines, vectors and proteins to produce and other requirements such as quality attributes
k Variability of the culture conditions and parameters k Quantities needed are variable, application dependent, with focus on
quality, time lines and cost-effectivenessk Regulatory authorities, e. g. FDA, EMA, supervise production processes
and products to ensure compliance and safety of the commercial product
A Service k Application Specialist – Full customer support
4 basal media k DoE run of 21 blends + 3 controls/center points
Full Protocols k Optimized process parameters
A 3 phases approach k Media Composition linked to Supply Agreement
Results in a unique custom medium and feed with a custom feed strategy.
CHOptimizer includes:– ~10 days of Application Specialist work– Media supplies including optimized media
and feeds– DoE software analytics– Media Spent Analysis (96 tests)– ambr15 and consumables
Featuresh Coupled DOE softwareh Reduce time to market with fully
optimized mediah Platform approach with control on process
and costs.h State-of-the-art multiple parallel single
use automated stirred-tank bioreactorsystem (ambr)
h No need of shipping cell line
Complementary products– Benchmarking Kit: Standard product
testing– Application Specialist Support– TAP ambr system– Media Optimization Tool Box– Cellca offerings
CHOptimizer
First run 24 blends from
4 base solutions
First run 16-39 blends from
4 base solutions
Use of ambr Second run in house
YES
NO
PHASE I
Upstream Applications
28
29
CHOptimizerThe Media Builder Box
How does it fit strategically?
Second run 3-4 blends from DoE
simulation 2-3 replicates
Process Optimization
Second run 3-4 blends from DoE
simulation 2-3 replicates
Third run in house
Third run Final solutions with
feedsProcess/Feed
StrategyOptimization
Third run Final solutions with
feeds
Media Optimized
Custom Optimized Media
PHASE II PHASE III
Recombinant Proteins
Value Added Media Optimization Service Media Benchmarking Kit CHOptimizer
Flexibility - ++ ++
Platform Approach -- -- +++
Plug and Play -- + +++
Time to market --- ++++ ++
Optimized Media +++ -- +++
DoE Approach (++) -- +++ Ambr + MODDE
30
ProNS0™ Protein Expression MediaThe NS0 cell line is widely used for recombinant mammalian protein expression. Some of the reasons to select this mouse myeloma platform are:– Forms stably producing hybrid cells with high levels of protein
production– Lacks ability to secrete endogenous antibody or antibody
fragments– Resists aggregation clumping in suspension culture– Lacks adverse protease activity on recombinant protein product
(in many cases)
BioWhittaker® ProNS0™ 1 Chemically Defined, Protein-free Media, together with ProNS0™ Lipid CD Supplement, are designed to meet a growing demand for optimized NS0 formulations.
Ordering Information
Cat. No. NA Cat. No. EU Product Description Size
BP12-773Q BP12-773Q ProNS0™ 1 CD 1 L
BP12-775J BP12-775J ProNS0™ Lipid CD 5 mL
ProNS0™ Mediak Two optimized formulas are available to cover the
broadest range of NS0 nutritional needsk Further maximize protein production by titration with
ProNS0™ Lipid CD Supplementk Protein-free and chemically defined formulationsk Product purification is simplifiedk Lot-to-lot consistency ensures dependable performancek Non-animal origin components reduce regulatory
burdens
This trio of NS0 products provides the answer to maximal protein productivity.
ProNS0™ Chemically Defined, Protein-free MediaTwo distinct formulas are offered to cover a broad range of nutritional needs. Titering the separate lipid supplement permits further optimization that can have a significant positive impact on protein production. Suggested starting concentration for optional addition of ProNS0™ Lipid CD Supplement is 5 mL per liter.
Basal Media 2ºC-8ºCLipid Supplement -10ºC--20ºC
Features of the standard product
Upstream Applications
31
Day 6 mg/mL
0
0.05
ProNS00 ™ 1 CD
Competitor 1 Competitor 2 Competitor 3
0.1
0.15
0.2
Figure 1. ProNS0™ Media outperform competitive media for recombinant IgG production in NS0 cells. Duplicate 125 mL shaker flasks were seeded at a density of 200,000 NS0 cells per mL in a 30 mL volume. Shake rate was 100 rpm. Cells were cultured in their respective test media for one passage prior to test initiation.
IgG
(mg/
mL)
ProNS0™ Media
Comparative protein production data are presented in Figure 1. All media were prepared according to manufacturer instructions. ProNS0™ Media were supplemented with 5 mL per liter of ProNS0™ Lipid CD. Competitive media either contained lipid or were supplemented with manufacturer’s companion lipid according to manufacturer recommendations. The batch cultures were not fed or further supplemented.
Samples were taken daily to measure viable cells and IgG concentration. Cell counts and viability were measured using a Vi-Cell™ Viable Cell Analyzer (Beckman Coulter). IgG concentrations were measured by HPLC.
The data show that both ProNS0™ 1 and 2 Chemically Defined, Protein-free Media yield superior protein production compared to competitive media.
Recombinant Proteins
32
UltraDOMA™
UltraDOMA™ Serum-free Hybridoma Medium is a formulation designed for the cultivation of murine, human, and chimeric hybridomas in batch culture and in hollow fiber bioreactors.UltraDOMA™ Medium is supplemented with recombinant human insulin, bovine transferrin and bovine albumin. The total protein concentration is 30 μg/ml. UltraDOMA™ Medium does not contain L-glutamine.Cells that are adapted for growth in UltraDOMA™ Medium can be maintained in the medium indefinitely and can be cryopreserved in UltraDOMA™ Medium supplemented with Cryoprotective Freezing Medium.
Applicationsk Hybridoma cell growthk Monoclonal antibody production
Partial List of Cell Types Cultivated with UltraDOMA™ Medium
Cell Type
Murine hybridomas
NS-1 derived myelomas
SP-2 derived myelomas
Human hybridomas (with 0.5% FBS)
Performance and Quality TestsUltraDOMA™ Medium contains no human-derived proteins
Ordering Information
Storage 2ºC-8ºC
Features of the standard product
Upstream Applications
Cat. No. NA Cat. No. EU Product Description Size
N/A BEBP12-723F UltraDOMA™ serum-free w/o L-Gln 500 mL 500 mL
BP12-723B BP12-723B UltraDOMA™ SF hybridoma medium, 500 mL 500 mL
33
UltraDOMA-PF™Partial List of Cell Types Cultivated with UltraDOMA-PF™ Medium
UltraDOMA-PF™ Protein-free Hybridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF™ Media are completely defined media and do not contain peptides or tissue extracts. The use of UltraDOMA-PF™ Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells.UltraDOMA-PF™ Media are designed for lab scale or industrial scale use. It is available as a 1X liquid or as a powder.L-glutamine and HEPES buffer are included in the formulation.
Applicationsk Hybridoma and myeloma growthk Monoclonal antibody production
Cell Type
Murine hybridomas
NS-1 derived myelomas
SP-2 derived myelomas
Human hybridomas (with 0.5% FBS)
Rat hybridomas
Some transfected Chinese Hamster Ovary (CHO) cell lines
Human lymphoid origin cells
Murine lymphoid origin cells
Ordering Information
Storage 2ºC-8ºC
Recombinant Proteins
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BP12-727F BP12-727F UltraDOMA-PF™ Hybridoma Medium, 500 mL 500 mL
34
ProDoma™
ProDoma™ Serum-free Hybridoma Media is designed for cultivation of murine, human, and chimeric hybridomas.
ProDoma™ Media are protein-free with a low amount of human recombinant insulin. All ProDoma™ Media include HEPES as well as sodium bicarbonate in the formulation.
Applicationsk Hybridoma cell growthk Monoclonal antibody production
Ordering Information
Storage 2ºC-8ºC
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BEBP02-029Q BEBP02-029Q ProDoma™ 1 serum-free medium, 1 L 1 L
BEBP02-032Q BEBP02-032Q ProDoma™ 3 woL-gln,PR w 0.1% Pluro F68 1 L 1 L
Upstream Applications
35
Viral vaccines are produced by viral infection of susceptible animal cells or by recombinant expression in suitable host cells. A variety of adherent cell lines are used for production of viral vaccines, mostly cells derived from kidneys, such as HEK293, Vero, MDCK, and BHK21, although others cell types can also be used (EB66, PerC6). Specialty media offered in this category have been designed to allow maximum cell growth and infection rate.
Viral Vaccines
Viral Vaccines
ProMDCK™ p.36
UltraMDCK™ p.37
ProVero™ 1 p.38
ProPer™ 1 p.39
PERMEXCIS® p.40
Pro239™ p.42
36
Ordering Information
Related Products
Cat. No. NA Cat. No. EU Product Name Size
12-749Q 12-749Q UltraMDCK™ 1 L
17-605 17-605 L-glutamine, 200 mM 100 mL
ProMDCK™ are serum-free media dedicated to the growth and infection of Madin-Darby Canine Kidney (MDCK) cells in all conditions, either for research of for further manufacturing purposes. ProMDCK™ has been optimized for both expansion and virus infection of MDCK cells in planar culture conditions (2D version) and in stirred bioreactors, bound onto microcarriers (3D version).
Applicationsk 2D version: MDCK cells growth in planar culture for research
(cell biology studies such as the mechanisms of passive salt, protein, lipid and drug transports, in vitro growth regulation and cytotoxicity studies), bioreactor seeding or QC tests
k 3D version: MDCK cells infection with virus (Influenza strains, poliovirus…) for vaccines manufacturing
ProMDCK™ media – 2D and 3D versions
Featuresk Easy to scale-up: Provides a suitable environment for MDCK
cells for all upstream steps (seeding, growth, infection) and manufacturing scales (T-flask to bioreactor) without any transition phase or adaptation needs.
k Efficiency: Increases doubling time and virus productivityk Regulatory-friendly: Serum-free media manufactured
following cGMP (21CFR820 guidelines) for maximum safety
Storage 2ºC-8ºC
Features of the standard product
Upstream Applications
NEW!
Cat. No. NA Cat. No. EU Product Description Size
12-924Q N/A ProMDCK™ (2D) 1 L
12-925Q N/A ProMDCK™ (3D) 1 L
37
UltraMDCK™
UltraMDCK™ Medium is a serum-free and chemically defined medium designed to support the growth of MDCK cells at low and high plating densities. UltraMDCK™ is an optimized basal medium supplemented with only two proteins – recombinant human insulin and bovine transferrin, manufactured following cGMP guidelines and yielding a very low protein content formulation.
The MDCK (Madin Carby Canine Cells) cell line was originated by S.H. Madin and N.B. Darby in 1958 from a kidney of an apparently normal, adult, female cocker spaniel. The cells are heteroploid with an epithelial like morphology.
MDCK cells grown in UltraMDCK™ medium are smaller and more densely packed than cells grown in the presence of serum. Moreover, cultures can stay confluent for at least two weeks without medium change. Cells will continue to grow from the monolayer forming spherical structures called “floaters”. “Floaters” can be harvested, pelleted by centrifugation and plated into fresh medium. They will re-attach and grow into a new monolayer.
A monolayer of MDCK cells is difficult to trypsinize especially when grown in a serum-supplemented medium. However, when grown in UltraMDCK™ medium, trypsinization becomes less difficult. UltraMDCK™ medium is offered in 1 liter plastic bottles as a “complete” medium. UltraMDCK™ medium has a shelf-life of one year.
Featuresk Efficiency
Superior cell growth characteristics beyond confluence and high viral productivity
k Regulatory-friendly A defined serum-free medium environment without the inconsistencies and concerns of serum and manufactured following cGMP with selected raw materials
k Simplified downstream process Easy purification procedures in manufacturing scale as it is chemically defined and contains only one protein and one peptide
ApplicationsMDCK cells are used in the isolation of influenza A and influenza B viruses. They have been found to be susceptible to vesicular stomatitis (Indiana strain) virus, vaccina, Coxsackie B-5, reovirus types 2, 3 and adenovirus types 4 and 51. MDCK cells have been used in functional studies such as the mechanisms of passive salt transport2, protein3, lipid4 and drug5 transport or in vitro growth regulation studies6 and cytotoxicity tests7. MDCK cells are currently used for marketed polio and flu vaccines manufacturing.
Ordering Information
Storage 2ºC-8ºC
Features of the standard product
Viral Vaccines
Cat. No. NA Cat. No. EU Product Description Size
BP12-749Q BEBP12-749Q UltraMDCK™ serum-free 1 L 1 L
38
ProVero™ 1 Serum-free Media
ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of Vero and MDCK kidney cells.ProVero™ 1 Medium includes HEPES and sodium bicarbonate buffer. The absence of proteins and the very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.Some Vero cell strains require additional supplementation with 5.0 μg/L rhEGF for optimal cell growth.
Applicationsk Virus productionk Recombinant protein production
Ordering Information
Storage 2ºC-8ºC
Features of the standard product
Upstream Applications
Cat. No. NA Cat. No. EU Product Name Size
12-749Q UltraMDCK™ 1 L
12-769E ProFreeze™ NAO 2X 100 mL
Related Products
Cat. No. NA Cat. No. EU Product Description Size
BEBP02-030Q BEBP02-030Q ProVero™ 1 NAO Medium for Vero Cells, 1 L 1 L
39
Related Products
Cat. No. NA Cat. No. EU Product Name Size
L-Glutamine 100 mL
ProFreeze™ CD (2X) Freeze Medium – Chemically 100 mL
Ordering Information
ProPer™ 1 Serum-free Medium – Chemically Defined, is an animal-origin component-free, chemically defined, serum-free medium for growth of human embryonic retinoblast cells (PER.C6® and related cell lines) in suspension. The medium contains a low amount of human recombinant insulin. HEPES and sodium bicarbonate are also present in the formulation.
Applicationsk Recombinant protein and virus productionk Growth of human embryonic retinoblast cells (PER.C6® and
related cell lines) in suspension
ProPer™ 1 Serum-free Media
PER.C6® Cells growing in ProPer™ 1 Medium.
Storage 2ºC-8ºC
Viral Vaccines
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BEBP02-028Q BEBP02-028Q ProPer™ 1 wo L-gln, PR w 0.1% Pluro F68 1 L 1 L
40
PER.C6® Technology is a Crucell proprietary human cell-based platform designed for large-scale production of recombinant proteins, including antibodies, vaccines and gene therapy products, under serum-free culture conditions. Cell lines have been immortalized with specific adenovector genes, leading to high viable cell densities and high PCDs (picogram/cell/day), resulting in high yields. PERMEXCIS® Virus Production Medium has been optimized for use with the PER.C6® Cell Line. PERMEXCIS® Medium is chemically defined, serum-free, low protein (<200 ng/mL), with Pluronic® F-68, and without L-glutamine or phenol red.
Ordering Information
Related Products
PERMEXCIS® Medium for Per.C6® cells
Featuresk Reduces regulatory hurdles with a non-animal origin statusk Maintains high viability (> 90%) at high cell densities for
superior performancek Achieves a maximum culture performance through a balanced
formulationk Saves validation time with no weaning required during growth
transition
Applicationsk Virus productionk Growth of human embryonic retinoblast cells (PER.C6® and
related cell lines) in suspension
Cat. No. NA Cat. No. EU Product Name Size
02-028Q 02-028Q ProPER-1 100 mL
17-605E 17-605E L-Glutamine 200 mM solution 100 mL
17-605F 17-605F L-Glutamine 200 mM solution 500 mL
17-605E/U1 17-605E/U1 UltraGlutamine™ | 200 mM 100 mL
17-605F/U1 17-605F/U1 UltraGlutamine™ | 200 mM 500 mL
12-769E 12-769E ProFreeze™ NAO 2+ 100 mL
Storage 2ºC-8ºC
Upstream Applications
PERMEXCIS® medium use is restricted only to PER.C6® Cell License Holders. Shipping will not be possible without license justification (please add license number on order or inform Sartorius customer services).
Per. C6® is a trademark of Crucell.
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BE02-039Q BE02-039Q GLS PERMEXCIS® virus production med, 1 L 1 L
41
Permexcis® Medium Provides Superior Growth and Cell Density with Lowest Population Doubling Times
Figure 1 PER.C6® Cells were cultured in 10 L roller bottles in several types of serum-free media. Cultures grown in Permexcis® Medium displayed a higher cell growth and the lowest population doubling time as compared to the competition.
0
1
1 2 3 4 5 6 7 8 9
2
3
4
5
6
7
Media Type Average PDT (h)Permexcis® Medium 23
Competitor A 27
Competitor B 26
Competitor C 28
Competitor D 30
Viab
le C
ell D
ensi
ty (1
06 vc/
mL)
Time (days)
Permexcis® Medium
Competitor A
Competitor B
Competitor C
Competitor D
Total cells Viability
PER.C6® Cells in Permexcis® Medium: 2 L iCeP PER.C6® Cells in Permexcis® Medium: 10 L iCeP
0
25 25
2.5 5.0 7.5 10.0 12.5 15.0
50 50
75 75
100 100
Figures 2 and 3 PER.C6® Cells grown in an intensified cell production process with Permexcis® Medium achieved a cell density above 100 ×106 (Vc/mL). PER.C6® Cells were grown in a 2 L bioreactor via perfusion method for 13 days and a 10 L bioreactor for 15 days respectively.
Cell
Den
sity
(×10
6 cel
ls/m
L)
Viability (%)
Time (days)
0
50 25
2.5 5.0 7.5 10.0 12.5 15.0
100 50
150 75
200 100
Cell
Den
sity
(×10
6 cel
ls/m
L)
Viability (%)
Time (days)
Viable Cell Density
Viral Vaccines
42
Pro293™ Serum-free MediaPro293™ Serum-free Media – Chemically Defined were optimized to support high-density growth and recombinant protein production in 293 neo-natal kidney cells (HEK293). They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal origin components.
Ordering Information
Applicationsk Pro293™ s Medium – for 293 cells growing in suspension culturek Pro293™ a Medium – for 293 cells growing in adherent culturek Recombinant protein production in 293 cells
Storage 2ºC-8ºC
Upstream Applications
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BP12-764Q BEBP12-764Q Pro293™a-CDM, for adherent cells, 1 L 1 L
N/A BEBP02-025Q Pro293™s-CDM, 1 L bottle 1 L
BP12-765Q BP12-765Q Pro293™s-CDM, for suspension cells, 1 L 1 L
43
Pro293™s easy adaptation to suspension culture
Pro293™s-CDM Commercial supplier A
12
10
8
6
4
2
0
Viab
le C
ells
/mL
(×10
5 )
0 1 2 3 4 5 6 7 8Days
Pro293™a supports adherent high-density cell growth
Pro293™a-CDM Commercial supplier B
60,000
50,000
40,000
30,000
20,000
10,000
0
Viab
le C
ells
(RFU
)
0 2 3 4 5Days
Viral Vaccines
44
In cell therapy treatments, also called regenerative medicine, cells are treated as the medical product, replacing missing or dysfunctional patient´s cells. Cells are taken from the donor, cultivated, expanded and eventually manipulated ex vivo before administrated to the patient. The provided cells can either be autologous (where the recipient is also the donor) or allogeneic (where the donor and recipient are distinct individuals). The cells used in cell therapy can be primary cells or from cell lines. The most important cells are hematopoietic cells (T or B lymphocytes, Dendritic or Natural killer cells), tissue cells (skin, etc.), and stem cells isolated from bone marrow, fat tissue or in very rare cases embryos.
Regenerative Medicine
Regenerative Medicine
X-Vivo™ p.46
L7™ hPSC Culture System
p.48
FGM-CD™ p.50
KGM-CD™ p.51
MSCGM-CD™ p.52
46
USP
Applicationsk Proliferation of PBL, TIL, LAKk Cultivation of human monocytes, macrophages, NK and
dendritic cellsk Cultivation of stem cellsk Cryopreservation and transplantation of human tissues
and organs Cell and Gene therapies
Featuresk Provides nutritionally complete and balanced environments
for a variety of primary and immortalized hematopoietic cells (PBL, LAK, TIL…)
k More efficient than classical media with serumk Serum free and defined media – regulatory friendly – for cell
or gene therapy applications
Proven efficiency on:k Human and murine LAK cellsk Peripheral blood monocytesk Human and murine TILk Human T cellsk Human and murine macrophagesk Human and murine hemotopoietic stem cells
Other human tissues
Technical Specifications:– Does not contain serum (serum free), exogenous growth
factors, artificial stimulators or undefined components– Contains human albumin but at a high quality level
(for injection) and licensed as excipient– Contains human transferrin but available also in a recombinant
form and from non-animal origin– Manufactured under current GMPs and listed with the FDA
in a product Master File
X-Vivo™ Media
The X-Vivo™ media formulations have been designed to support the generation of hematopoietic cells including like peripheral blood lymphocytes (PBL), lymphokine activated killer (LAK) cells and tumor infiltrating lymphocytes (TIL) from healthy donors or patients. These media can also support the ex vivo growth of human monocytes, macrophages, granulocytes, natural killer (NK) cells or other hematopoietic cells. They are a suitable serum free environment for primary cells but also for stem cells, cancer cells or different but related hematopoietic cell lines.
The three different formulations of X-Vivo™ (X-Vivo™ 10, 15 and 20) are similar and can be suitable for the different applications. We recommend testing samples of the different formulations for each application. These media have been referenced in a lot of scientific publications and some comparative studies have proven that X-Vivo™ is a high efficiency serum free medium suitable for numerous different cell types.
Defined media specifically designed by Lonza for high efficiency hematopoietic cell cultivation
Storage 2ºC-8ºC
Upstream Applications
Features of the standard product
47
Ordering Information
X-VIVO™ product range (with native human transferrin)
Products with recombinant transferrin
Cat. No. NA Cat. No. EU Product Name Product Description Size
BE02-055Q BE02-055Q X-Vivo™ 10 without gentamicin nor red phenol (04-743) with recombinant
transferrin
1 L Bottle
BE02-053Q BE02-053Q X-Vivo™ 15 with gentamicin and phenol red (04-418) with recombinant
transferrin
1 L Bottle
BE02-054Q BE02-054Q X-Vivo™ 15 without gentamicin nor phenol red (04-744) with recombinant
transferrin
1 L Bottle
Regenerative Medicine
Cat. No. NA Cat. No. EU Product Description Size
08-879H 08-879H STD X-Vivo 15 w/o gentamicin and pr, 5 L 5 L
BEBP04-743Q BEBP04-743Q X-Vivo™ 10 w/o Gentamycin w/o PR 1 L 1 L
BP04-380Q BEBP04-380Q X-Vivo™ 10 w/ Gentamycin and PR, 1 L 1 L
BP04-418Q BEBP04-418Q X-Vivo™ 15 w/ Gentamycin and PR, 1 L 1 L
N/A BEBP04-418F X-Vivo™ 15 w/ Gentamycin and PR, 500 mL 500 mL
BP04-744Q BEBP04-744Q X-Vivo™ 15 w/o Gent or Phenol Red, 1 L 1 L
BP04-448Q BEBP04-448Q X-Vivo™ 20 w/ Gentamycin and PR, 1 L 1 L
BEBP02-055Q BEBP02-055Q X-Vivo™ 10 (04-743) contains rec transf -1 L 1 L
BEBP02-053Q BEBP02-053Q X-Vivo™ 15 (04-418) contains rec transf, 1 L 1 L
BEBP02-054Q BEBP02-054Q X-Vivo™ 15 (04-744) contains rec transf, 1 L 1 L
48
L7™ hPSC Culture System
L7™ system is a culture platform (medium, matrix, subculture reagent, and cryopreservation medium) that supports every-other-day feeding of human Pluripotent Stem Cells (PSCs) in a defined system. The L7™ System is a xeno-free and fully defined system for the maintenance and expansion of human ESCs and iPSCs. The L7™ hPSC Culture System is a robust culture system that supports every other day feeding of pluripotent stem cells. As a complete solution, L7™ includes different products that are very safe and efficient if used together:
– L7™ hPSC Matrix– L7™ hPSC Medium BulletKit™ – L7™ hPSC Passaging Solution– L7™ hPSC Cryosolution
Ordering Information
Featuresk Robust performance and compatibility with most linesk Reduced workload and increased flexibility by supporting
every-other-day feedingk Efficient differentiation into all three germ layersk Easy transition to clinical applicationsk Complete system for the maintenance of human ESCs
and iPSCs
Upstream Applications
Use all of the L7™ PSC Culture System components for proven performance with PSC growth, expansion, and maintenance.
L7™ hPSC Basal Medium and Medium SupplementThis medium was specifically designed and formulated with a basal medium and a supplement of growth factors to support long-term growth and maintenance of undifferentiated PSCs.
L7™ hPSC MatrixLonza’s recominant hPSC Matrix is xeno-free and defined. When used in conjunction with L7™ hPSC Basal Medium, it is proven to support efficient attachment, maintain pluripotency and stable karyotype for over 40+ passages in many hPSC lines.
L7 hPSC Passaging SolutionLonza’s L7™ hPSC Passaging Solution is a chemically-defined, non-enzymatic cell detachment formulation that gently dislodgesmulticellular colony fragments without the need for mechanical manipulation. This user friendly method simplifies routine maintenance of your valuable hESC and iPSC lines.
L7™ hPSC Cryopreservation SolutionLonza’s Cryopreservation Solution supports efficient recovery of cryopreserved hPSCs under xeno-free and defined conditions.
L7™ hPSC Medium BulletKit™ L7™ hPSC Matrix L7™ hPSC Passaging Solution L7™ hPSC Cryosolution
Colony Appearance
Colony Selection and Expansion
Banking Characterization
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
04-1193E N/A L7™ hPSC Passaging Solution 100 mL
00215550 N/A L7™ hPSC Cryosolution 50 mL
00215549 N/A L7™ hPSC Matrix 1 mg
00215506 N/A L7™ hPSC Media BulletKit™ 500 mL Basal medium + 5 mL Supplement
04-1191F N/A L7™ hPSC Basal Medium 500 mL
04-1192J N/A L7™ hPSC Supplement 5 mL
49
Regenerative Medicine
Reviving and Priming Reprogramming
Colony Appearance
Colony Selection and Epansion
Banking and Characterization
1 Workflow
7 Components
1 Provider
L7™
1 Clonetics™ and Poietics™ Primary Cells
2 L7™ Reprogramming Bundles
3 Nucleofector™ Technology
4 L7™ hPSC Medium BulletKit™
5 L7™ hPSC Matrix
6 L7™ hPSC Passaging Solution
7 L7™ hPSC Cryosolution
50
FGM-CD™ Fibroblast Growth MediaFeaturesk Serum-free and chemically defined medium for expansionk Maintain the fibroblasts characteristicsk Promote collagen production
Ordering Information
Collagen Production by NHDF-Ad grown in FGM-CD™
140
120
100
80
60
40
20
0Donor I
Colla
gen
(μg)
FGM-CO™ DMEM+10%FBS
Donor II Donor III Donor IV
FGM-CO™
Fibroblast
CD90™
Collagen I
DMEM+10%FBS
Basal medium: 2°C to8°C
Supplements: -10°C to -20°C
Upstream Applications
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
199019 199019 FBM-CD™
199020 199020 FGM-CD™ SinglQuots kit Kit
00199041 00199041 FBM-CD™ Bullet Kit Kit
51
TheraPEAK™ KGM-CD™ Keratinocyte Growth Media
Featuresk Contains only non-animal orgin components
k KGM-CD™ medium supports both neonatal and adult keratinocytes
k Supports formation of an excellent barrier resulting in high TEER values
k Coating plates with fibronectin or other non-animal derived matrix
material is necessary for successful keratinocyte isolations with KGM-CD™
medium
Ordering Information
Basal medium: 2°C to8°C
Supplements: -10°C to -20°C
Regenerative Medicine
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
CC4455 CC4455 BulletKit KGM-CD™ Kit
CC-4456 CC-4456 BulletKit KGM-CD™ EA CC3255+CC4455 Kit
52
TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth Media
TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth
Medium – Chemically Defined (with L-glutamine, without
phenol red and antibiotics).
Ordering Information
Featuresk Supports human mesenchymal stem cell growth
(multi-potent adult stem cells)
k Differentiation into osteoblasts, chondrocytes and adipocytes
k Chemically defined and serum-free
k For further manufacturing or laboratory use
Poietics™ hMSC grown in
MSCGM-CD™ Medium
Poietics™ hMSC grown in DMEM
with 10% FBS
Basal medium: 2°C to 8°C,
Supplements: -10°C to -20°C
Regenerative Medicine
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
190632 190632 MSCGM-CD™ bulletkit Kit
192125 192125 MSCGM-CD™ SingleQuots Kit Kit
190620 190620 MSCBM-CD™ 500 mL 500 mL
Sartorius Lonza offers a full portfolio of standard or custom buffer products to optimize your process needs in all key DSP steps and applications including:– Cell harvest– Concentration | Diafiltration– Capture– Polishing – Virus clearance | Inactivation– Bulk drug substance preparation– Final fill
As well as:– Organ preservation and transport – Tissue conservation
While the emphasis in upstream cell culture processing is growth and titer optimization, the downstream focus is purification, concentration, and preservation. These process demands can result in the need for very large buffer volumes and Sartorius Lonza is well equipped to work with you on scalability and minimizing the burden associated with transport logistics. Custom buffers will be prepared to your exact specifications of composition, molarity, pH, and quality. Global manufacturing facilities follow cGMP (21CFR820) and ISO (9001:2008) regulatory protocols to ensure a consistent, reliable, and uninterrupted supply of your powdered and liquid buffer in your preferred packaging. Whether you need a standard off-the-shelf buffer or customized solution, Sartorius Lonza has an experienced team to help guide your selection and development.
Downstream Steps
Downstream Steps
The Downstream Buffer Offering
p.54
Buffer Packaging and Options
p.56
DSP Buffers p.10
PBS p.58
DPBS p.59
Hanks p.60
Earle’s p.60
Hepes p.61
Other Buffers p.61
WFI p.62
54
Downstream Steps
Sartorius offers a wide range of process buffers for purification of monoclonal antibodies, recombinant proteins, vaccine and antibody drug conjugates.
The Downstream Buffer Offering
Buffer needs and volumes can become very large as processes grow and Sartorius Lonza is well equipped to work with you on minimizing the burden associated with transport logistics.Sartorius offers an extensive array of downstream processing buffers for protein, cellular, and viral-based therapeutics. Appropriate for the techniques you perform and for each steps of your techniques (see above).
Any buffers can be prepared to your exact specifications of composition, molarity, pH… This includes:
Harvest Concentration | Diafiltration Capture Polishing Virus Clearance | InactivationBulk Drug Substance Preparation
Fill and Finish
Centrifugation Crossflow Filtration Affinity Chromatography CHR CEX VR Filtration Formulation Final FormulationWFI Equilibration Buffer Equilibration Buffer WFI Formulation Buffer Formulation Buffer
Dynamic Body Feed Flushing Buffer Wash Buffer Wash Buffer Post-Flush Buffer WFI
Washing Buffer Elution Buffer Elution Buffer Sterile FiltrationCrossflow Filtration Regeneration Buffer Regeneration Buffer UV-C Innactivation WFI
WFI UF | DF Sanitization Buffer Sanitization Buffer Flush Buffer Post-Flush Buffer
Flushing Buffer WFI Storage Buffer Storage Buffer
Washing Buffer Equilibration Buffer Dilution Buffer Dilution Buffer Solvent | Detergent Innactivation Controlled Freeze and ThawDiafiltration Buffer Solvent Buffer Freezing Buffer
Depth Filtration Sanitization Buffer Low-pH Virus Inactivation Membrane Adsoption AEX Flush Buffer WFI
WFI Acid Sanitization Buffer
Post-Flush Buffer Base Flushing Buffer UF | DFDilution Buffer Equilibration Buffer WFI
Post-Flush Buffer Diafiltration Buffer
Strip Buffer Sanitization Buffer
mAbs Generic Downstream Process Buffer Usage
k Water for injection (WFI) k Strong Acids and Bases k Balanced Salt Solutions (BSS) – Acetate buffers – Citrate buffers – Potassium buffersk Tris Formulations k Phosphate Buffered Saline (PBS)
and DPBSk Cell Harvesting buffers k Glycine
k Freezing mediak Dedicated formulation bufferk Physiological solutionsk …
As well as other buffers:k Hepesk Earlesk Hanksk D Glucose …
55
Buffers
Harvest Concentration | Diafiltration Capture Polishing Virus Clearance | InactivationBulk Drug Substance Preparation
Fill and Finish
Centrifugation Crossflow Filtration Affinity Chromatography CHR CEX VR Filtration Formulation Final FormulationWFI Equilibration Buffer Equilibration Buffer WFI Formulation Buffer Formulation Buffer
Dynamic Body Feed Flushing Buffer Wash Buffer Wash Buffer Post-Flush Buffer WFI
Washing Buffer Elution Buffer Elution Buffer Sterile FiltrationCrossflow Filtration Regeneration Buffer Regeneration Buffer UV-C Innactivation WFI
WFI UF | DF Sanitization Buffer Sanitization Buffer Flush Buffer Post-Flush Buffer
Flushing Buffer WFI Storage Buffer Storage Buffer
Washing Buffer Equilibration Buffer Dilution Buffer Dilution Buffer Solvent | Detergent Innactivation Controlled Freeze and ThawDiafiltration Buffer Solvent Buffer Freezing Buffer
Depth Filtration Sanitization Buffer Low-pH Virus Inactivation Membrane Adsoption AEX Flush Buffer WFI
WFI Acid Sanitization Buffer
Post-Flush Buffer Base Flushing Buffer UF | DFDilution Buffer Equilibration Buffer WFI
Post-Flush Buffer Diafiltration Buffer
Strip Buffer Sanitization Buffer
With our downstream offerings the priority is on quality, flexibility, and the assurance that there are no interruptions to supply so your required product is there when you need it, in your preferred format.
The process begins with approved and specified raw materials obtained from qualified vendors. Our insistence on superior-quality materials includes complete NAO (non-animal origin) traceability and our Water for Injection (WFI) systems meet both US and European Pharmacopoeia standards. Standard testing on finished media products includes tests for sterility, osmolality, pH, and endotoxin content.
Global manufacturing facilities follow cGMP (21CFR820) and ISO (9001:2008) regulatory protocols to ensure a consistent, reliable supply of your powdered and liquid media.
Every product we manufacture is backed by the expertise of our professional customer service, technical service and field representatives. Our staff has been trained to meet your service needs whether they are in custom packaging, specialized delivery, follow-up or consulting. We will help our clients match their formulations with the packaging they need to fit their unique system. We can also aid in addressing our client's specific inventory needs and issues. From the time of purchase to the day of shipment, our team will stand by our clients and their product.
Buffer quality systems
56
Downstream Steps
Buffer solutions are available in both liquid or powder formats and a corresponding range of convenient packaging options. Whether you need a standard off-the-shelf buffer or a customized solution, Sartorius Lonza has an experienced team to help guide your selection and development.
Buffer Packaging and Options
The Processk Transfer the pre-weighted dispensing bags or liner
in the mixing room (they are wrapped in overlay to facilitate access to class 8 room)
k Add the desire water quantity to get a required volume of buffer
k Check the pHk Filter as recommended in the protocol
The Benefitk Do not have to purchase individual raw materials
and store itk Do not have to qualify raw material suppliers and
run QC/QA test of new raw materials batchesk Will not have to weigh the powder at the desired
quantityk Will not have to mix the various components of
the buffers in a container to be transported in your mixing room
k Will not have to adjust pH (just validate it)k Will not have to define a filtration protocol
(it will be provided to you)
k A custom buffer composition at your exact specification
k pH pre-adjustedk Powder quantity adjusted to the exact quantity
required for your desired liquid volumek Fully qualified raw material
k Buffer in powder format from 100 g bottles to 20 KKgs drums, for you to weigh, rehydrate, adjust pH and filter in your premises
Enhanced Buffer Formatsk Powder buffers ready for rehydratation either:– Packaged in dispensing bags to be used with
closed mixing system– Or packaged in “liners” for mixing drums to be
used with an open mixing solution
k Ready-to-use liquid buffers manufactured under cGMP (21CFR820) and ISO (9001:2008) available in bottles from 0,5 L to 5 L as well as in bags from 1 L to 1000 L
The Traditional Offering
NEW!
57
Buffers
Buffer Powder in Liners for Open Drum Mixing Solutions
Pre-weighed, pre pH-adjusted powder buffer in a liner
Liner | bag for powder transportation:– Connection to common mixing systems– Tubings for sterile filtration and final fill– Predetermined pH and osmolarity
Installation of the liner into a mixing drum and removal of the clamp
Installation of the liner into a mixing drum and removal of the clamp
Final Storage Bag for the sterile buffer
Powder Bag:– Connection to Lonza Filling Station– Robust for transportation– Connection to common mixing Systems
Closed Mixing System:– Semi-sterile preparation of the medium– No pH adjustment + osmolarity
Optional transfer to storage bag
Storage bag if required:– Customer decision– Quality control of the
final product (sterility, pH, osmolarity, conductivity, endotoxin)
Buffer Powder in Dispensing Bags for Closed Mixing Solution
Features of pre-weighed buffer in dispensing bags and liners:Reduce QA for raw material inspection as well as finished goods k Risk avoidance and cost saving
No powder weighing steps k No need of weighing room, saves time and reduces errors
Complete solution =Full re-suspension | pH and osmolarity adjustment | filtration protocol provided
k Result consistency
Less dust or no dust k Safer
Fully compatible solution for either open drum mixing or closed mixing systems k Ease of use
Easy to store k Storage at ambient | lower storage space up to the mixing step
Same buffer available in liquid (0,5 L to 10 L) or in small drums k Consistency from early PD to large manufacturing scale
58
Phosphate Buffered Salines
Cat. No. Size Without Phenol Red Without Calcium or Magnesium With Hepes
17-516F 500 mL • •
17-516Q 1 L • •
17-517Q 1 L • •
17-737E 100 mL •
17-737F 500 mL •
BE02-017F 500 mL
12-624E 100 mL
17-711E 100 mL •
Ordering Information
Storage 15ºC-30ºC
Downstream Steps
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
17-517Q BE17-517Q PBS-10x w/o Ca, Mg 1 L 1L
17-516Q/12 BE17-516Q/12 PBS-1X w/o Ca, Mg 12 × 1 L 12 × 1 L
17-516F/12 BE17-516F/12 PBS-1X w/o Ca, Mg 12 × 500 mL 12 × 500 mL
17-516Q BE17-516Q PBS-1X w/o Ca, Mg 1 L 1 L
17-516F BE17-516F PBS-1X w/o Ca, Mg 500 mL 500 mL
17-516F/24 17-516F/24 PBS-1X, w/o Ca, Mg 24 × 500 mL 24 × 500 mL
BE02-017F BE02-017F PBS-EDTA 500 mL 500 mL
59
Buffers
Dulbecco’s Phosphate Buffered Saline
Cat. No. Size Without Phenol Red With Calcium and Magnesium With Glucose With Sodium Pyruvate
17-513F 500 mL • •
17-513Q 1 L • •
17-512F 500 mL •
17-512Q 1 L •
04-479Q 1 L • • • •
17-515F 500 mL (10 ×) •
17-515Q 1 L (10 ×) •
Ordering Information
Storage 15ºC-30ºC
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BE15-512D BE15-512D STD DPBS Powder w/o Ca, Mg 10 L 10 L
N/A BE15-512F STD DPBS Powder w/o Ca, Mg 50 L 50 L
N/A BE15-512K STD DPBS Powder w/o Ca, Mg 1000 L 1000 L
17-512Q/12 BE17-512Q/12 DPBS 0.0095M(PO4) w/o Ca and Mg, 12 × 1 L 12x1 L
17-512Q BE17-512Q DPBS 0.0095M(PO4) w/o Ca and Mg, 1 L 1 L
17-512F BE17-512F DPBS 0.0095M(PO4) w/o Ca and Mg, 500 mL 500 mL
17-512F/12 BE17-512F/12 DPBS 0.0095M(PO4) w/o Ca&Mg, 12 × 500 mL 12x500 mL
17-515Q BE17-515Q DPBS-10x w/o Ca, Mg 1 L 1 L
17-515F BE17-515F DPBS-10X w/o Ca, Mg 500 mL 500 mL
04-479Q 04-479Q DPBS-1X w/ Ca & Mg Glucose and Pyr, 1 L 1 L
17-513Q BE17-513Q DPBS-1X w/ Ca, Mg 1 L 1 L
17-513F BE17-513F DPBS-1X w/ Ca, Mg 500 mL 500 mL
N/A BE17-513F/12 DPBS-1X w/ Ca, Mg BOX of 12 bottles 12 × 500 mL
17-512F/24 17-512F/24 DPBS-1X w/o Ca++, Mg++ 24 × 500 mL 24 × 500 mL
60
Balanced Salt Solutions
Earle’s Balanced Salt Solution
Hank’s Balanced Salt Solution
Cat. No. Size With Phenol Red With Calcium and Magnesium
10-508F 500 mL • •
10-508Q 1 L • •
10-543F 500 mL •
10-543Q 1 L •
10-527F 500 mL •
10-547F 500 mL
04-315Q 1 L
Ordering Information
Ordering Information
Storage 15ºC-30ºC
Storage 15ºC-30ºC
Downstream Steps
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
08-003A 08-003A STD HANKS’ BSS, W/O CA,MG OR PR, 4 L BG 4 L
10-527F/12 10-527F/12 HANKS’ BSS, W/O Phenol Red 12 × 500 mL 12 × 500 mL
10-508Q 10-508Q Hanks’ BSS, with Phenol Red, 1 L 1L
10-508F BE10-508F HBSS w/ Phenol Red 500 mL 500 mL
10-543F BE10-543F HBSS w/ PR w/o Ca, Mg 500 mL 500 mL
N/A BE10-543F/12 HBSS w/ PR w/o Ca, Mg BOX of 12 bottles 12 × 500 mL
10-527F BE10-527F HBSS w/o Phenol Red 500 mL 500 mL
10-547F BE10-547F HBSS w/o PR, Ca or Mg 500 mL 500 mL
04-315Q 04-315Q HBSS, w/o Ca++,Mg++ or Phenol Red, 1 L 1 L
10-543Q 10-543Q HBSS, w/o Ca++,Mg++ w/ phenol red, 1 L 1 L
Cat. No. NA Cat. No. EU Product Description Size
N/A BE02-027F EBSS w/ HEPES low NaHCO3, 500 mL 500 mL
N/A BE10-502F EBSS w/ Phenol Red 500 mL 500 mL
61
Buffers
HEPES BufferOrdering Information
Ordering Information
Other Buffers
Storage 15ºC-30ºC
Cat. No. NA Cat. No. EU Product Description Size
BE17-737E 17-737E HEPES Buf,1M Stk in normal saline, 100 mL 100 mL
17-737F 17-737F HEPES Buffer,1M in normal saline, 500 mL 500 mL
Cat. No. NA Cat. No. EU Product Description Size
12-747F 12-747F UltraSaline A without Phenol Red, 500 mL 500 mL
10-539B 10-539B Dextrose Gelatin Veronal Buffer, 500 mL 500 mL
12-624E 12-624E Veronal Buffer (5X) 100 mL 100 mL
17-711E BE17-711E Versene (EDTA) 0.02% 100 mL 100 mL
Citrate buffer
Acetate Buffer
Potassium phosphate buffer
Glycine buffer
D Glucose buffer
Tris buffer
Ammonium Sulfate
Sodium Hydroxide
Histidine
Sodium chloride
62
Buffers
WFIDefinitionOur off-the-shelf WFI products are available in 500 mL or 1 L bottles, 20 L and 200 L bags. We can also provide made to-order WFI in custom packaging options to fit your application or direct ready-to-use solutions (Cell Culture Media, Buffers, Organ Transplant Solutions). Delivery time: 24 hours to 1 week from reception of Purchase Order (Add an extra week for Asia). Facilities follow cGMP 21CFR820 and are certified ISO9001 and ISO13485.
Product DescriptionWater For Injection produced in bulk; meeting USP and EP Pharmacopeia criteria; filtered using Sartopore II filters into primary packaging (bottles and | or bags)– Available in 500 mL or 1 L bottles, in 20 L or 200 L Bags off
the shelf
Bulk packaged for commercial use elsewhere, not for parenteral administration. Our WFI Quality Water is aseptically processed from a validated Water For Injection System that meets current USP and EP specifications in the loop. Our WFI Quality Water is not to be confused with Sterile Water for Injection which is intended for use in extemporaneous prescription compounding, and as a diluent for injectable parenteral products. In addition, Sterile Water for Injection must be packaged in single dose containers not larger than 1 liter in size.
This product is manufactured aseptically under cGMP 21CFR820 conditions by a validated sterile filtration method and tested according to current U.S.P. and E.P. This product has been produced for laboratory | manufacturing use. Use for in vitro diagnostic procedures has not been established. It is the end user’s responsibility to ensure that the final products meets the requirements of the application.
FeaturesVery Low Endotoxin level and
Sterilek Minimization of Contamination
High Quality made under cGMP
21CFR820 conditionsk Regulatory Friendly and Risk
Mini mization
No need for internal installation of
WFI loopk Significant Savings
ApplicationSubject to the proviso, customer checks quality according to CoA for suitability to his | her needs UpStream: Media Preparation and HydrationDownstream: Buffer PreparationUpStream & Downstream: Cleaning, Washing, Rinsing (filters, equipment)
Features of the standard product
Ordering Information
Cat. No. NA Cat. No. EU Product Description Size
08-199D N/A STD WFI Comm. Use Elsewhere, 100 L bag 100 L
08-199E N/A STD WFI Comm. Use Elsewhere, 200 L drum 200 L
08-199ES N/A STD WFI Sample Bag – 2 L bag 2 L
08-199F N/A STD WFI Comm. Use Elsewhere, 20 L bag 20 L
N/A BE02-038P0.5 Bulk Packaged ster.filtered WFI – 500 mL 500 mL
N/A BE02-038P20 Bulk Packaged sterile filtered WFI-20 L 20 L
N/A BE02-038P200 Bulk Packaged sterile filtered WFI-200 L 200 L
17-724Q BE17-724Q USP WFI bulk Sterile filtered 1 L 1 L
17-724F BE17-724F USP WFI bulk Sterile filtered 500 mL 500 mL
N/A BE17-724F/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 500 mL
N/A BE17-724Q/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 1 L
Reagents
ReagentsThe Sartorius Lonza Cell Culture Reagents portfolio includes products such as amino acids, antibiotics, and assorted processing agents. These products, which can be manufactured under the same cGMP conditions as our other products, are routinely used in both research and manufacturing applications.
Chemicals used to prepare cell culture reagents are purchased according to raw material qualifications from approved suppliers. Each lot must meet established component specifications before Quality Assurance release. All liquid cell culture reagents are manufactured using Water for Injection (WFI) quality water and all liquid products are filtered through pharmaceutical grade sterilizing filters. If there is a desired cell culture reagent that is not listed in our standard portfolio, we can create a custom reagent specific for your requirements.
Sodium bicarbonate p.64
Sodium pyruvate p.64
Insulin p.65
Trypsin p.65
L-Glutamine p.66
Ultra Glutamine p.66
Penicillin p.67
Amphotericin p.68
Gentamycin p.68
ProFreeze™ p.69
Anticlumping Agents p.70
64
Reagents
Buffering Solutions or Chemicals
Sodium Bicarbonate
Sodium Pyruvate
Ordering Information
Ordering Information
Storage 15ºC-30ºC
Storage 2ºC-8ºC
Cat. No. NA Cat. No. EU Product Description Size
15-613I BE15-613I STD Sodium Bicarbonate powder 500 g 500 g
17-613E BE17-613E Sodium Bicarb. 7,5% sol 100 mL 100 mL
Cat. No. NA Cat. No. EU Product Description Size
13-115E BE13-115E Sodium Pyruvate Solution 100 mM, 100 mL 100 mL
65
Reagents
Amino-Acids, Peptides, Proteins & Enzymes
Insulin
Trypsin
Ordering Information
Ordering Information
Storage -10ºC--20ºC
Storage -10ºC--20ºC
Cat. No. NA Cat. No. EU Product Description Size
02-033E BE02-033E STD Insulin Human recomb. 5 mg/mL 100 mL 100 mL
02-033E20 BE02-033E20 STD rh Insulin, 5 mg/mL, 20 mL bottle 20 mL
02-033Q N/A STD Human Recombinant Insulin 5 mg/mL, 1 L 1 L
BE02-033E80 N/A Human Rec Insulin 5 mg/mL 80 mL 80 mL
Cat. No. NA Cat. No. EU Product Description Size
17-160F 17-160F Trypsin(10 +) 2.5% /HBSS w/o Ca, Mg, 500 mL 500 mL
BE02-007E BE02-007E Trypsin/EDTA 10 × 100 mL 100 mL
N/A BE02-034E Trypzean/EDTA solution 100 mL bottle 100 mL
17-160E BE17-160E Trypsin(10 +) 2.5% in HBSS w/o Ca, Mg 100 mL 100 mL
N/A BE17-161E/12 Trypsin-Versene (EDTA) 12 × 100 mL 12 × 100 mL
17-161E BE17-161E Trypsin-Versene (EDTA) Mix (1 +) 100 mL 100 mL
17-161F BE17-161F Trypsin-Versene (EDTA) Mix (1 +) 500 × mL 500 mL
66
Reagents
Vitamins & Other Nutrients
Ultra Glutamine
L-GlutamineOrdering Information
Ordering Information
Storage -10ºC--20ºC
Storage 15ºC-30ºC
Cat. No. NA Cat. No. EU Product Description Size
15-605G BE15-605G L-GLUTAMINE POWDER 25 G 25 g
N/A BE15-605X(3000) STD L-Glutamine Powder 3000 g 3000 g
17-905C 17-905C L-Glutamine 200mM Hybridoma test, 50 mL 50 mL
17-605E BE17-605E L-Glutamine 200mM 100 mL 100 mL
17-605F BE17-605F L-Glutamine 200mM 500 mL 500 mL
17-605C 17-605C L-Glutamine 200mM 50 mL 50 mL
N/A BE17-605E/12 L-Glutamine 200mM BOX of 12 bottles 12 × 100 mL
Cat. No. NA Cat. No. EU Product Description Size
BE17-605E/U1 BE17-605E/U1 Ultraglutamine I 200mM 100 mL 100 mL
N/A BE17-605E/U1/12 Ultraglutamine I 200mM BOX of 12 bottles 12 × 100 mL
N/A BE04-684E UltraGlutamine II 100 mL 100 mL
N/A BE04-684E/12 UltraGlutamine II BOX of 12 bottles 12 × 100 mL
67
Reagents
Antibiotics
Cat. No. Size 5,000 Units Penicillin
–5,000 μg Streptomycin
10,000 Units Penicillin
–10,000 μg Streptomycin
20,000 Units Penicillin
–20,000 μg Streptomycin
25,000 Units Penicillin –
25,000 μg Streptomycin
25 μg/mL
Amphotericin B
With
L-glutamine
17-603E 100 mL •
17-602E 100 mL •
17-602F 500 mL •
17-718R 25 ×
4.5 mL
• •
09-757F 500 mL •
17-745H 20 mL • •
17-745E 100 mL • •
Related Products Page
MycoZap™ Antibiotics 164
MycoAlert™ PLUS Mycoplasma Detection Kit 161
Penicillin-Streptomycin Mixtures
Ordering Information
Related Products
Storage -10ºC--20ºC
Cat. No. NA Cat. No. EU Product Description Size
17-602E DE17-602E Pen 10.000 UI/mL Strep 10.000 UI/mL 100 mL
17-602F DE17-602F Pen 10.000 UI/mL Strep 10.000 UI/mL 500 mL
17-603E DE17-603E Pen 5.000 U/mL Strep 5.000 U/mL 100 mL
09-757F 09-757F Pen/Strep stock, 20K/20K 500 mL 500 mL
17-745E 17-745E Pen/Strep/Fungizone 10K/10K/25 μg, 100 mL 100 mL
17-745H 17-745H Pen/Strep/Fungizone 10K/10K/25 μg, 20 mL 20 mL
17-718R 17-718R Pen/Strep/L-Gln 25K/25K/200 mM, 25 + 4.5 mL 25 + 4.5 mL
N/A DE17-603E/12 Penicillin/Strep 5000U/mL BOX of 12 BOT 12x100 mL
N/A DE17-602E/12 Penicillin10.000 UI/mL BOX of 12 bottles 12x100 mL
68
Reagents
Antibiotics
AmphotericinOrdering Information
GentamicinOrdering Information
Storage -10ºC--20ºC
Storage 15ºC-30ºC
Cat. No. NA Cat. No. EU Product Description Size
17-836R 17-836R Amphotericin B, antifungal 250 ug/mL, 20 mL 20 mL
17-836E 17-836E Amphotericin B, antifungal 250ug/mL, 100 mL 100 mL
Cat. No. NA Cat. No. EU Product Description Size
17-519Z 17-519Z Gentamicin 10 mg/mL 1 × 10 mL screw cap 10 mL
17-519L 17-519L Gentamicin 10 mg/mL 10 × 10 mL screw cap 10 × 10 mL
17-528Z 17-528Z Gentamicin 50 mg/mL 1 × 10 mL crimp-top 10 mL
17-518Z 17-518Z Gentamicin 50 mg/mL 1 × 10 mL screw cap 10 mL
17-518L 17-518L Gentamicin 50 mg/mL 10 × 10 mL screw cap 10 × 10 mL
N/A BE02-012E Gentamycin Sulfate 100 mL, 10 mg/mL Gent 100 mL
69
Reagents
Additives & Freezing Media
ProFreeze™ CD (2X), NAO Freeze Medium Chemically Defined, Non-animal Origin Freezing Medium
ProFreeze™ CD (2X), NAO Freeze Medium – Chemically Defined is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS). However, it is used to greatest advantage with cells cultured in a serum-free and animal component-free environment. This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use. One bottle will make 117.6 mL of complete 2X concentrated freezing medium after the addition of 17.6 mL DMSO. For best results, keep on ice during use.
Ordering Information
Storage 2ºC-8ºC
Features of the standard product
Cat. No. NA Cat. No. EU Product Description Size
BP12-769E BP12-769E ProFreeze-CDM, NAO, 2x Freeze Med, 100 mL 100 mL
70
Reagents
Additives & Freezing Media
Anti-Clumping Agents
Chinese Hamster Ovary (CHO) cells are widely used in large-scale culture. These cells have conventionally been grown as attached cultures, but they will also grow in suspension, a property which has allowed for relatively simple scale up in stirred tank and airlift bioreactors. Unfortunately, some CHO-derived lines which have been adapted to suspension growth can form large, tightly bound aggregates in culture.
Why is clumping a problem?– For batch suspension cultures, cell aggregation hinders accurate cell counting, monitoring and control of the cellular environment– Transport of nutrients to, and products from, the cells may be impaired– Cluster formation dramatically influences the growth behavior of the cells– Cells within aggregates show a strongly reduced specific proliferation rate– Shear forces exerted on large aggregates cause a considerably higher specific death rate than those exerted on single cells, reducing
the specific growth rate up to 50 %
Approaches to solving the problem of cell aggregation have proven to be less than satisfactory, until now… Lonza introduces Anti-clumping Agents A and B, two ready-to-use solutions designed to minimize CHO cell clumping in suspension cultures. Both solutions are made exclusively of non-animal origin components.
– Anti-clumping Agent A is a buffered, concentrated solution of Dextran sulfate, and recommended usage is at 100 × dilution– Anti-clumping Agent B is a buffered, concentrated solution of ferric citrate, and recommend use it at 100 × or 200 × dilution
As a result of using Anti-clumping Agents from Lonza, clumping of cells cultured in serum-free medium is minimized, leading to a significant increase in cell density and productivity.
Control image shows clusters of CHO CRL-11397 cells grown in suspension culture.
CHO CRL-11397 cells, grown with Anti-clumping Agent A (Dextran sulfate),do not aggregate in suspension culture.
CHO CRL-11397 cells, grown with Anti-clumping Agent B (Ferric citrate), do not aggregate in suspension culture.
Features of the standard product
71
Reagents
Resolution of Clumping Problems in CHO Cells Leads to a Signicant Increase in the Cell Density and Productivity
Units/m
L
0 0
5,0000.2
0.4
1.4
1.6
1.8
2.0
0.6
0.8
1.0
1.2
15,000
10,000
20,000
25,000
30,000
35,000
Avg.
RFU
PowerCHO™ 3(12-772Q)
Day 3 – growth Day 4 – growth Day 5 – growth Day 6 – growth Day 7 – prod
FeCit – 1/200(BERDO48E)
FeCit – 1/100(BERDO48E)
Dextran Sulfate – 1/100(BERD047E)
Ordering Information
As noted in the graph, the effect of both agents on CHO cells seeded in PowerCHO™ 3 Medium is obvious. Cell density is significantly increased by the addition of Anti-clumping Agent B after just three days in culture, while, after seven days on culture, both Anticlumping Agents A and B exhibit increased productivity of 20 up to 35 %.
Cat. No. NA Cat. No. EU Product Description Size
BE02-058E BE02-058E STD Anti clumping agent A 100 mL 100 mL
Store at 15° – 30° C
72
Services
Media Services
In addition to a broad standard classical and chemically-defined product portfolio, the Sartorius Lonza media services team is able to provide cell culture solutions specific to a client’s unique processing needs. These services leverage the extensive knowledge base of our commercial, regulatory, and scientific experts and are available across the entire spectrum of bioprocessing unit operations (USP/DSP/FF), formats (liquid/powder), and clinical applications.
Media ServicesCustomization p.74
Support p.74
Validation p.74
Quality p.76
Packaging and Shipping p.80
Services
Sartorius Lonza will work collaboratively with clients to produce unique media solutions. This service option is production-focused and the perfect choice for clients who have known, desired media composition and outcome expectations. Additionally, clients may request specific suppliers for critical raw materials or rely upon Sartorius Lonza’s established global network of qualified suppliers. Sartorius will follow the clients requested parameters and project scope. It is also possible to request customized packaging, media formats, and release assays.
Customization
ValidationProducts that are supplied for further manufacturing use are manufactured in qualified and validated equipment. Most standard catalogue items have a specific shelf life indicated on the Certificate of Analysis. For products that do not have shelf life data (custom products), we offer shelf life studies (standard or accelerated) on request.Other services like filtration studies, leachable | extractable studies, can be offered with support from our Confidence® group.
The combined resources of the Sartorius | Lonza collaboration allow for a dedicated team of media professionals with a broad range of expertise including R&D, clinical manufacture, product commercialization, engineering, and media development. Certificates of Analysis (C of A’s) for standard products are available online at http://www.lonza.com/coaTechnical questions on standard media products can be directed to Scientific Support at [email protected]
Custom and specialized media projects will be assigned a project team composed of a Sales Representative, a Media Project Manager, and a Media Application Specialist.
Support
75
Services
Services
Quality
Sartorius’ manufacturing partners, Lonza Walkersville, Inc. and Lonza Verviers, Inc. are currently engaged in the manufacture of over 1,500 products for pharmaceutical and biotechnology applications such as disease diagnosis, biomedical research, production of diagnostics and therapeutics, and quality control testing of pharmaceuticals and medical devices for the presence of bacterial contaminants.Manufacture of these products, specifically cell culture media, reagents, and buffers, follow the protocols and compliance specifications of the following industry regulations:– 21CFR820– ISO 9001: 2008 certified– ISO 13485: 2003 certified
Further information on these protocols and document management are available upon request. Quality agreements and audits are available at the customer’s discretion.
USP Sterility – Tests are performed on representative samples using the membrane filtration procedure in accordance with the US Pharmacopoeia or EP Pharmacopoeia. Culture media used are fluid thioglycollate medium (FTM) and trypticase soy broth (TSB). All tested media must pass growth promotion testing before use. The test samples are filtered and the filters are immersed in FTM and TSB. TSB cultures are incubated at 22.5°C +/- 2.5°C. FTM cultures are incubated at 32.5°C +/- 2.5°C. The cultures are incubated for 14 days, during which they are periodically examined and sterility results are recorded.
Moisture Content –This is a powder specific test. We are committed to offering the same high quality powdered media as you have come to expect with our liquid media. We utilize a unique micronization manufacturing process, which produces powder in a controlled environment, guaranteeing maximum stability and minimum degradation due to chemical oxidation.
Endotoxin – Products are tested for endotoxin content using the Kinetic-QCL™ Assay. Test dilutions used are screened for inhibition and enhancement in the Kinetic-QCL™ Assay. Endotoxin levels for these products are available on Certificates of Analysis.
Available final product testing includes the following:
Cell Growth Promotion – Media and supplements are tested for their growth-promoting properties using primary, diploid, and heteroploid cell lines unless other cells are appropriate based on the product use. Results are obtained using the Lowry protein method or manual cell count to assess cell replication. Visual screening of cultures is also done to ensure characteristic cell morphology and lack of toxicity. Additional testing for toxicity, function and/or activity may also be conducted if one or more is required in the product release specifications.
Chemistries (pH and Osmolality) – Tests are performed on representative samples from each lot using routinely calibrated equipment. Osmolality is determined by means of the highly repeatable freezing point method.
Shelf Life –Samples from each lot of standard product are retained and stored at label temperature in the event follow-up testing is required. Subsequent testing may be in response to your inquiry or for shelf life studies. Shelf life data is not included but is available for some products and upon request. Shelf life is generally between 6 and 24 months for liquid products and 24 months for powder products.
76
Quality
Release Testing and Documentation
Liquid media Powder media
Testing Options
Sterility Standard N/A
pH Standard N/A
Osmolality Standard Standard
Endotoxin Standard N/A
Cell Growth Promotion Standard on CHO medias N/A
Mycoplasma On Request N/A
Moisture N/A Standard
Bioburden N/A Standard
Shelf Life On Request On Request
Documentation
CofA and raw material CoA Standard Standard
In order to maintain consistent quality in sterile cell culture media products, strict control of each production lot is essential. Written procedures in accordance with current Good Manufacturing Practices (cGMPs) provide quality control from start to finish for each product.
To verify the conformity of material to established specification throughout the manufacturing process, inspections and testings occur during the following four stages: – Raw material receipt– In-process manufacturing, labeling and packaging– Finished product– Post-manufacture for shelf life studies.
77
78
Services
Attributes Product for research Non-GMP (“Pilot”) catalogue products and custom Protein | Vaccine production media for cGMP production
Product use For research use onlyFor upstream | downstream biopharma process development
For upstream | downstream Biopharma clinical phase 3 or commercial production
Quality Assurance
QA System ISO 9001 ISO 9001, ISO 13485 ISO 9001, ISO 13485 and cGMP 21CFR821DHR–Device History Record – – h
PI Sheet–Process Instruction Sheet (in SAP) – – h
MSDS–Material Safety Data Sheet h h h
CoA– Certificate of Analysis h h h
TSE | BSE statement On request h h
CoO– Certificate of Origin On request h h
Master Table– Theoretical data with all approved sources. – On request h
Batch Table– Production real data – On request h
Specification sheet– Internal Testing References N.A. N.A. –
Labels Request (for custom products) N.A. N.A. h
CPR– Custom Product Request N.A. h h
Change Notification None Systematic and proactive (timelines 6 weeks) Systematic and proactive (timelines 6 weeks)
Quality Control Product Approval and Release No QA inspection of documentsAll products are released following an approved | controlled an inspection of all quality documents harmonized between both sites
All products are released following an approved | controlled QA inspection of all quality documents harmonized between both sites
Product Storage and Shipment
Shipment conditions Theoretical shipment conditionsHarmonized shipping condition between sites supported by validation data
Harmonized shipping condition between sites supported by ASTM validation data
Handling of customer demands
Handling of Customer Quality Agreements N.A. 21 day Lonza Turnaround Time 21 day Lonza Turnaround TimeCustomer Complaints N.A. 7 day Lonza Turnaround Time 7 day Lonza Turnaround Time
Handling of Customer Audits N.A.
Upon receipt of customer’s audit request Lonza/Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days
Upon receipt o customer’s audit request Lonza | Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days
Confidentiality N.A. Confidential information shared under CDA Confidential information shared under CDA
Quality Charter
79
Quality
Attributes Product for research Non-GMP (“Pilot”) catalogue products and custom Protein | Vaccine production media for cGMP production
Product use For research use onlyFor upstream | downstream biopharma process development
For upstream | downstream Biopharma clinical phase 3 or commercial production
Quality Assurance
QA System ISO 9001 ISO 9001, ISO 13485 ISO 9001, ISO 13485 and cGMP 21CFR821DHR–Device History Record – – h
PI Sheet–Process Instruction Sheet (in SAP) – – h
MSDS–Material Safety Data Sheet h h h
CoA– Certificate of Analysis h h h
TSE | BSE statement On request h h
CoO– Certificate of Origin On request h h
Master Table– Theoretical data with all approved sources. – On request h
Batch Table– Production real data – On request h
Specification sheet– Internal Testing References N.A. N.A. –
Labels Request (for custom products) N.A. N.A. h
CPR– Custom Product Request N.A. h h
Change Notification None Systematic and proactive (timelines 6 weeks) Systematic and proactive (timelines 6 weeks)
Quality Control Product Approval and Release No QA inspection of documentsAll products are released following an approved | controlled an inspection of all quality documents harmonized between both sites
All products are released following an approved | controlled QA inspection of all quality documents harmonized between both sites
Product Storage and Shipment
Shipment conditions Theoretical shipment conditionsHarmonized shipping condition between sites supported by validation data
Harmonized shipping condition between sites supported by ASTM validation data
Handling of customer demands
Handling of Customer Quality Agreements N.A. 21 day Lonza Turnaround Time 21 day Lonza Turnaround TimeCustomer Complaints N.A. 7 day Lonza Turnaround Time 7 day Lonza Turnaround Time
Handling of Customer Audits N.A.
Upon receipt of customer’s audit request Lonza/Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days
Upon receipt o customer’s audit request Lonza | Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days
Confidentiality N.A. Confidential information shared under CDA Confidential information shared under CDA
Services
Individually, our Business Units are industry-leading suppliers for the application they serve, such as filtration, fluid handling and fermentation. Combining forces collectively enhances our strength in production while reinforcing Sartorius Stedim Biotech | Lonza as a global supplier for the biopharmaceutical industry. We can therefore assist in designing and providing the best products for our customers.With decades of experience and a rich heritage in engineering, we offer packaging solutions answering your specific needs providing excellence in customer service, product quality and innovation solutions.We want to protect our products in the best possible way with high-quality packaging – on the inside as well as the outside. We always place particular value on the highest standards of safety and security, in order to offer efficient and flexible solutions in a market that is both challenging and constantly changing.
Leveraging Sartorius and Lonza Strengths
Packaging
Media & Buffer product storage and shipping conditions vary. While some can be stored at ambient temperature (uncontrolled), others might need to be stored at Room Temperature (15-30°C), Refrigerated Temperature (2-8°C) or Frozen Temperature (-10°C/-20°C).
Appropriate packaging is provided to maintain optimal shipping conditions.We have years of experience in shipping small volumes as well as large volumes of our products (in liquid or powder form) at different temperatures all around the globe. Dedicated transport can be offered at specific costs. Please contact your Sartorius representative or Media & Buffer Specialist for further information.
Shipping
Features and Benefits Skilled staff, a careful choice of materials, pioneering infrastructure, efficient internal process management and a transparent communication policy guarantee reliability, delivery times, the highest quality standards and strict cost control.
Liquids With a comprehensive range of bottles and bags we can offer standard off-the-shelf or customized containment from 0.5 liters up to 1000 liters.
Powders We offer a wide range of powder containers from 10g bottles up to several 100 kg. We also provide ready-to-use solutions such a pre-weighted powder in bags, media kits, etc.
Industry leader in batch size scalability
k Liquid: 0.5 L to 10,000 L batch sizePowder: 7 kg to 7,000 kg batch size
Dual global manufacturing sites
k Assurance of supply
Sartorius Lonza k Fit-for-purpose and ready to use solutions adding value to your processes
Wide range of primary and secondary packaging
Answering your specific needs
80
81
Shipping & Packaging
Shipping Solutions for Media & Buffers | Liquid Form
0,5 to 1 L 1 to 2,5 L 2 to 20 L 50 L 100 L 200 L 500 L 1000 L
Bottle h h
LDPE 2D Pillow Bag h h
Drum h h h
LDPE 3D Bag in Container h h h h
Stainless Steel Palletank h h h h
Plastic Palletank h h
82
Packaging
Shipping Solutions for Media & Buffers | Powder Form
10 g to 2 kg 5 kg to 20 kg 5 kg to 20 kg 2 kg to 20 kg 500 g to 2 kg
Bucket h
Drum h
Powder Bag h h
Flexel Tankliner h
Applicable for Media & Buffers
Media & Buffers
Media & Buffers
NEW!
Buffers (pre-weighted
solution for closed mixing
systems)
NEW!
Buffers (pre-weighted
solution for open mixing
systems)
Classical Media
Classical MediaClassical media are produced using well-known public recipes (published formulations). Nevertheless, Lonza manufactures this type of media with great care, following cGMP guidelines and in accordance with ISO:9001. A Device Master Record (DMR) is prepared for every medium produced. It defines the production procedures–from receipt of raw materials to final product release, the environmental and processing controls required, as well as product specifications (including packaging and labeling), ensuring batch-to-batch consistency. Like all other BioWhittaker™ Media, classical media are manufactured with the highest-grade raw materials, most often EP or USP compliant and sourced from qualified and approved suppliers. Water for Injection (WFI) quality water is consistently used to produce any liquid media product. Indeed, we rely on our excellence and expertise to offer you the safest, most reliable and most consistent products.
BME (Basal Medium Eagle)
p.84
DMEM (Dulbecco’s Modified Eagle’s Medium)
p.84
EMEM (Eagle Minimum Essential Medium)
p.86
DMEM:F12 Medium p.88
GMEM (Glasgow Minimum Essential Medium)
p.88
Grace’s Insect p.89
Ham’s p.89
IMDM (Iscove’s Modified Dulbecco’s Medium)
p.90
L-15 (Leibovitz) p.90
M199 p.91
Mc Coy’s p.91
PC-1™ p.92
HL-1™ p.94
RPMI p.96
William’s E p.97
Richter’s p.97
NCTC-109 p.97
UltraMEM™ p.98
84
Classical Media
Basal Medium Eagle (BME)A minimal medium suitable for a variety of cell types. It is a historical precursor to Minimum Essential Medium (MEM).
Cat. No. NA Cat. No. EU Product Name Product Description Size
BE12-105F BE12-105F Basal Medium Eagle (BME) With Earle’s BSS, without L-glutamine 500 mL
Ordering Information
DMEM is used in a wide range of mammalian cell culture applications. The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.
Dulbecco’s Modified Eagle’s Medium (DMEM)
Cat. No. Size Without
L-Glutamine
Without Sodium
Pyruvate
Without
PhenolRed
With HEPES With 1.0g/L
glucose
With 4.5g/L
glucose
Powder Hybridoma
Screened
With UG I*
12-614F 500 mL • • •
12-614Q 1 L • • •
12-604F 500 mL • •
12-604Q 1 L • •
BE12-604F/U1 500 mL • • • •
12-914F 500 mL • • • •
12-917F 500 mL • • • •
12-707F 500 mL • • •
12-708F 500 mL • • • •
12-709F 500 mL • • • •
12-733F 500 mL • •
12-733Q 1 L • •
12-741F 500 mL •
* With UltraGlutamine I
Storage 15ºC-30ºC
Storage 2ºC-8ºC
Features of the standard product
Features of the standard product
85
Classical Media
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-614F BE12-614F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine 500 mL
12-614Q 12-614Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine 1 L
12-604F BE12-604F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine 500 mL
12-604Q 12-604Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine 1 L
BE12-604F/U1 BE12-604F/U1 Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with UltraGlutamine™ I 500 mL
12-914F BE12-914F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine, hybridoma tested 500 mL
12-917F BE12-917F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or phenol red 500 mL
12-707F BE12-707F Dulbecco's Modified Eagle Medium (DMEM) With 1.0 g/L glucose, without L-Glutamine 500 mL
12-708F BE12-708F Dulbecco‘s Modified Eagle Medium (DMEM) With 1.0 g/L glucose, without L-Glutamine, with 25 mM HEPES 500 mL
12-709F BE12-709F Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine, with 25 mM HEPES 500 mL
12-733F BE12-733F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or sodium pyruvate 500 mL
12-733Q 12-733Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or sodium pyruvate 1 L
12-741F BE12-741F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine, without sodium pyruvate 500 mL
Ordering Information
86
Classical Media
Eagle Minimum Essential Medium (EMEM) Storage 2ºC-8ºC
Cat. No. Size W/
L-glutamine
W/
Sodium
Pyruvate
W/o
Phenol
Red
W/
HEPES
W/
UG I*
W/
HBSS
W/
EBSS
W/
NEAA
W/
Pen-strep-
amph B
W/o Na-
bicarbonate
W/o
Calcium
W/
Nucleosides
12-169F
(Alpha)
500 mL • • •
BE02-002F
(Alpha)
500 mL • • • • •
12-611F 500 mL • •
12-611Q 1 L • •
12-125F 500 mL •
12-125Q 1 L •
12-662F 500 mL • • •
12-662Q 1 L • • •
12-136F 500 mL • •
12-136Q 1 L • •
12-736E 100 mL • • • • •
12-736F 500 mL • • • • •
12-684F
(10x)
500 mL •
12-668E
(2x)
100 mL • •
06-174G 450 mL • • • •
12-127F 500 mL •
12-137F 500 mL • •
04-719Q
(Joklik’s)
1 L • • •
* With UltraGlutamine I
Features of the standard product
MEM Eagle is suitable for a diverse spectrum of mammalian cell types. It is available with either Hanks’ or Earle’s salts. MEM-Hanks’ (12-127F or 12-137F) does not require a CO2 enriched atmosphere. Joklik’s modification is intended for suspension culture.
87
Classical Media
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-169F BE12-169F Minimum Essential Medium -
Alpha Eagle with Earle‘s BSS
Without L-Glutamine, deoxyribonucleosides and ribonucleosides 500 mL
BE02-002F BE02-002F Minimum Essential Medium –
Alpha Eagle
With UltraGlutamine™ I, deoxyribonucleoside and ribonucleosides 500 mL
12-611F BE12-611F Minimum Essential Medium –
Eagle with Earle‘s BSS
With L-Glutamine 500 mL
12-611Q 12-611Q Minimum Essential Medium –
Eagle with Earle‘s BSS
With L-Glutamine 1 L
12-125F BE12-125F Minimum Essential Medium –
Eagle with Earle‘s BSS
Without L-Glutamine 500 mL
12-125Q 12-125Q Minimum Essential Medium –
Eagle with Earle‘s BSS
Without L-Glutamine 1 L
12-662F BE12-662F Minimum Essential Medium – Eagle Without L-Glutamine, with non-essential amino acids and sodium pyruvate 500 mL
12-662Q 12-662Q Minimum Essential Medium – Eagle Without L-Glutamine, with non-essential amino acids and sodium pyruvate 1 L
12-136F BE12-136F Minimum Essential Medium –
Eagle with Earle‘s BSS
Without L-Glutamine, with 25 mM HEPES 500 mL
12-136Q BE12-136Q Minimum Essential Medium –
Eagle with Earle‘s BSS
Without L-Glutamine, with 25 mM HEPES 1 L
12-736E 12-736E Minimum Essential Medium –
Eagle with Earle‘s BSS
Cell culture maintenance medium 12-136 with non-essential amino acids,
L-Glutamine, 25 mM HEPES, 10 μg/mL gentamicin, 50 units/mL penicillin,
50 μg/mL streptomycin, 2.5 μg/mL amphotericin B, and 2.0% heat-inactivated
FBS
100 mL
12-736F 12-736F Minimum Essential Medium –
Eagle with Earle‘s BSS
With L-Glutamine 500 mL
12-684F BE12-684F Minimum Essential Medium –
Eagle with Earle‘s BSS (10X)
Without L-Glutamine or NaHCO3 500 mL
12-668E 12-668E Minimum Essential Medium –
Eagle with Earle‘s BSS (2X)
Without L-Glutamine or phenol red (virus plaquing medium) 100 mL
BE12-668F BE12-668F Minimum Essential Medium –
Eagle with Earle‘s BSS (2X)
Without L-Glutamine or phenol red (virus plaquing medium) 500 mL
06-174G BE06-174G Minimum Essential Medium –
Eagle with Earle‘s BSS
With non-essential amino acids, and L-Glutamine, without calcium 450 mL
12-127F BE12-127F Minimum Essential Medium –
Eagle with Hank's BSS
Without L-Glutamine 500 mL
12-137F 12-137F Minimum Essential Medium – Eagle Without L-Glutamine, with 25 mM HEPES 500 mL
04-719Q 04-719Q Minimum Essential Medium – Eagle
Joklik’s Formulation
For suspension cultures, with L-Glutamine, without calcium 1 L
Ordering Information
88
Classical Media
DMEM:F12 MediumDMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well – suited for clonal density cultures.
Cat. no. Size Without L-
Glutamine
With HEPES With 3.151g/L
Glugose
Powder With UG I*
12-719F 500 mL • •
12-719Q 1 L • •
BE04-687Q 1 L •
BE04-687F/U1 500 mL • • •
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-719F BE12-719F Dulbecco‘s Modified Eagle Medium:F12
(DMEM:F12)
1:1 mixture with 3.151 g/L glucose, L-Glutamine and 15 mM
HEPES
500 mL
12-719Q 12-719Q Dulbecco‘s Modified Eagle Medium:F12
(DMEM:F12)
1:1 mixture with 3.151 g/L glucose, L-Glutamine and 15 mM
HEPES
1 L
BE04-687F/
U1
BE04-687F/
U1
Dulbecco‘s Modified Eagle Medium:F12
(DMEM:F12)
1:1 mixture with 3.151 g/L glucose, with UltraGlutamine™ I,
without HEPES
500 mL
BE04-687Q BE04-687Q Dulbecco‘s Modified Eagle Medium:F12
(DMEM:F12)
1:1 mixture with 3.151 g/L glucose, with UltraGlutamine™ I,
without HEPES
1 L
Ordering Information
Glasgow Minimum Essential Medium (GMEM)Designed to support BHK-21 cells.
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-739F BE12-739F Glasgow Minimum Essential Medium With L-Glutamine 500 mL
Ordering Information
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Features of the standard product
89
Classical Media
Grace’s Insect Medium
Ham’s Medium
Classical insect cell media.
Developed for low density (clonal) growth of CHO cells.
Cat. No. NA Cat. No. EU Product Name Product Description Size
04-457F 04-457F Grace‘s Insect Medium Without FBS, with yeastolate, Lactalbumin hydrolysate or gentamicin 500 mL
04-501Q 04-501Q Grace‘s Complete Insect Medium
(TNM-FH)
With 10% heat-inactivated FBS, yeastolate, Lactalbumin hydrolysate, and
gentamicin
1 L
04-649F 04-649F Grace‘s Insect Medium Without FBS, with yeastolate, Lactalbumin hydrolysate, and gentamicin 500 mL
Ordering Information
Cat. No. NA Cat. No. EU Product Name Product Description Size
BE02-014F Ham‘s F10 Medium With UltraGlutamine™ I, without thymidine 500 mL
12-615F BE12-615F Ham‘s F12 Medium With L-Glutamine 500 mL
12-618F BE12-618F Ham‘s F10 Medium With L-Glutamine 500 mL
Ordering Information
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Features of the standard product
Features of the standard product
90
Classical Media
Iscove’s Modified Dulbecco’s Medium (IMDM)IMDM is suitable for fast growing cells. All formulas contain HEPES for added buffering.
Cat. No. Size Without L-Glutamine With HEPES Hybridoma Screened
12-722F 500 mL •
12-722Q 1 L •
12-726F 500 mL • •
12-726Q 1 L • •
12-915F 500 mL • •
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-722F BE12-722F Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES 500 mL
12-722Q 12-722Q Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES 1 L
12-726F BE12-726F Iscove‘s Modified Dulbecco‘s Medium (IMDM) Without L-Glutamine, with 25 mM HEPES 500 mL
12-726Q 12-726Q Iscove‘s Modified Dulbecco‘s Medium (IMDM) Without L-Glutamine, with 25 mM HEPES 1 L
12-915F BE12-915F Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES, hybridoma tested 500 mL
Ordering Information
L-15 (Leibovitz) MediumDeveloped for fast growing tumor cells, this formula does not require a CO2 enriched atmosphere. The bicarbonate free medium is buffered with elevated levels of amino acids.
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-700Q 12-700Q L-15 Leibovitz's Medium Without L-Glutamine 1 L
12-700F BE12-700F L-15 Leibovitz's Medium Without L-Glutamine 500 mL
12-669E 12-669E L-15 Leibovitz's Modified
Medium (2X)
(2X) except L-tyrosine (1X), without L-Glutamine or phenol red (virus plaquing
medium)
100 mL
Ordering Information
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Features of the standard product
Features of the standard product
91
Classical Media
McCoy’s 5A MediumDesigned for human lymphocyte culture.
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-168F BE12-168F McCoy‘s 5A Medium With L-Glutamine and 25 mM HEPES 500 mL
12-688F BE12-688F McCoy‘s 5A Medium With L-Glutamine 500 mL
Ordering Information
Medium 199Medium 199 was originally formulated for chick embryo fibroblast culture. These four formulations require a CO2
enriched atmosphere.
Cat. No. Size With L-Glutamine With HEPES With HBSS With EBSS With 1.4g/L Na-
bicarbonate
With 2.2 g/L
Na-bicarbonate
12-117F 500 mL • • • •
12-117Q 1 L • • • •
12-118F 500 mL • • • •
12-119F 500 mL • • •
12-109F 500 mL • • •
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-109F BE12-109F Medium 199 With Hank‘s BSS, L-Glutamine, and 1.4 g/L NaHCO3 500 mL
12-117F BE12-117F Medium 199 With Earle‘s BSS, L-Glutamine, 25 mM HEPES, and 2.2 g/L NaHCO3 500 mL
12-117Q 12-117Q Medium 199 With Earle's BSS, L-Glutamine, 25 mM HEPES, and 2.2 g/L NaHCO3 1 L
12-118F BE12-118F Medium 199 With Hank's BSS, L-Glutamine, 25 mM HEPES, and 1.4 g/L NaHCO3 500 mL
12-119F BE12-119F Medium 199 With Earle‘s BSS, L-Glutamine, and 2.2 g/L NaHCO3 500 mL
Ordering Information
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Features of the standard product
Features of the standard product
92
Classical Media
PC-1™ Serum-free Medium Chemically Defined, General Purpose Serum-free Medium
PC-1™ Serum-free Medium – Chemically Defined is a low-protein medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1™ Medium contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ Medium is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
Cell Line Source Cell Type
HeLa Human Epithelial carcinoma, cervix
HTB-72 Human Malignant melanoma, epithelial-like
HTB-4 Human Bladder tumor
WI-38 Human Lung, diploidw
MRC-5 Human, male Embryonal lung, fibroblast-like
BHK-21 Syrian hamster Kidney, fibroblast-like
CHO-K1 Chinese hamster Ovary, epithelial-like
NRK Rat Normal kidney, epithelial/fibroblast-like
C6 Rat Glioma, primary
T9 Rat Glioma
ARL6T Rat Normal liver
3T3 Mouse Embryonic, fibroblast-like
STO Mouse Transformed fibroblast
VERO African green monkey Fibroblast
MDCK Dog Madin Darby canine kidney, epithelial-like
SIRC Rabbit Cornea
Partial List of Cell Cultures Cultivated with PC-1™ Serum-free Medium – Chemically Defined
Cultivation of primary and anchorage-dependent cells
Applications
Medium: 2°C to 8°C,
Supplement: -10°C to 20°C
Features of the standard product
93
Classical Media
Other Cell Types/Sources
Source Cell Type
Human
Neuroblastoma, foreskin fibroblast, bladder carcinoma, renal papillary collecting tubule (primary), colon epithe lium (primary), colon
carcinoma (primary)
Rat
Dermal fibroblast (primary), mammary carcinoma (primary), neonatal normal cardiac muscle (primary), thyroid epithelium (primary),
astrocytes (primary)
Baboon (Paprocynocephalus) spinal ganglia
Swine Testes cell
Bovine: Kidney
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L-Glutamine 146
ProFreeze™ CD (2X) Freeze Medium – Chemically Defined 132
Cat. No. NA Cat. No. EU Product Name Product Description Size
77232 77232 PC-1™ Serum-free Medium –
Chemically Defined
Complete medium system, including frozen supplement, without
L-Glutamine. For primary adherent cells.
2 × 500 mL
Ordering Information
94
Classical Media
Partial lists of cell cultures cultivated with HL-1™ Serum-free Medium – Chemically Defined
Cell Line Source Cell Type
U937 Human Macrophage
RaJi Human B lymphoblastic
MCF-7 (NIH) Human Breast carcinoma
MCF-7 (MCF) Human Breast carcinoma
NIH ZR-75 Human Breast carcinoma
COLO 302 HSR Human Colon carcinoma
J82 Human Bladder carcinoma
SW 1738 Human Bladder carcinoma
SW780 Human Bladder carcinoma
CCL 119 Human Lymphoid
CCL 213 Human Burkitt lymphoma
C91 / PL Human T lymphoma
Undesignated Human Astrocytoma
Undesignated Human Hepatoma
MOLT-3 Human Acute lymphoblastic leukemia
MOLT-4 Human Acute lymphoblastic leukemia
NAMALWA Human Burkitt lymphoma
THP-1 Human Monocytic leukemia
BB88 Mouse Erythroid (leukemia)
P815 Mouse Macrophage
Transformed and Established Cell Lines
Cell Line Source Cell Type
WeHi3 Mouse Monocyte
JLS-V5 Mouse Spleen cell
70Z-3 Mouse Pre-B lymphoma
70Z / 3.12 Mouse B lymphoma
S49 and variants Mouse T lymphoma
RAW309F1.1 Mouse T lymphoma
WeHi7 Mouse T lymphoma
I-10 Mouse Leydig-tumor
EL4 Mouse T lymphoma
RL1 Mouse T lymphoma
BW5147.3 Mouse T lymphoma
LBRM-33 Mouse T lymphoma
Friend leukemia Mouse Leukemia
C57BL6 Mouse Embryo (C57 × DBA)
L5178Y Mouse Lymphoma (DBA / 2)
VERO African green
monkey
Fibroblast
MDCK Dog Madin Darby canine kidney
CHO K1 Hamster Chinese hamster ovary
(epithelial-like)
GCL2 Hamster | mouse B lymphoma × Normal B
HL-1™ Serum-free MediumChemically Defined Hybridoma Medium
HL-1™ Serum-free Medium – Chemically Defined is a culture medium containing less than 30 μg protein per mL. Components of HL-1™ Medium include HEPES buffer, insulin, transferrin, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids and proprietary stabilizing proteins. HL-1™ Medium contains no bovine serum albumin or other undefined protein mixtures. HL-1™ Medium supports the serum-free growth of various hybridomas, including those derived from P3X63Ag8.653 and Sp2/0-Ag14 myelomas, as well as other differentiated cells of lymphoid origin.
Serum-free growth of hybridomas and differentiated cells of lymphoid origin
HL-1™ Chemically Defined FBS Substitute (100X) is a chemically defined medium additive that can be used to replace serum or significantly reduce its concentration in a variety of basal media. It contains less than 30 μg protein per mL when diluted 1:100 in medium and it does not contain bovine serum albumin or other undefined protein ingredients.
HL-1™ FBS Substitute (100X) – Chemically Defined
Applications
Serum-free 2ºC-8ºCHL-1™ Fetal Bovine Serum 15°C to 30°C
Features of the standard product
95
Classical Media
Cat. No. NA Cat. No. EU Product Name Product Description Storage Conditions Size
77227 77227 HL-1™ Fetal Bovine Serum Substitute (100X) –
Chemically Defined
15°C to 30°C 10 mL
77201 BE77201 HL-1™ Serum-free Medium for Hybridoma and
Hematopoietic Cells – Chemically Defined
Without L-Glutamine 2°C to 8°C 2 × 500 mL
CE marked according to IVD Directive 98 / 79 / EC.
Related Products Page
L-Glutamine 146
ProFreeze™ CD (2X) Freeze Medium – Chemically Defined 132
Ordering Information
Hybridoma Source Fusion Partner
8A1 Human CLLC
undesignated Human WI-L2-729-HF2
undesignated Human LICR-LON-HMY2
HB44 Mouse Sp2 / 0-Ag14
HB45 Mouse Sp2 / 0-Ag14
HB56 Mouse NS-1
HB59 Mouse NS-1
HB60 Mouse P3X63Ag 8.653
53-7.313 Mouse NS-1
MI / 9.3.4HL-2 Mouse NS-1
MI / 70.15.1 Mouse NS-1
ARB Mouse Hybridoma
P3U Mouse P3X63Ag 8.653
BCS12 Mouse P3X63Ag 8.653
BCS 2002 Mouse P3X63Ag 8.653
undesignated Mouse NS-1
undesignated Mouse P3X63Ag 8.653
Hybridomas
Primary Cells
Cell Type
Human peripheral blood mononuclear cells
Mink lymphocytes
Human fetal adrenal
Human blood monocytes
Human peripheral blood T lymphocytes
Hybridoma Source Fusion Partner
TIB 175 Rat/mouse S194
TIB 104 Rat/mouse NS-1
TIB 105 Rat/mouse NS-1
TIB 109 Rat/mouse NS-1
TIB 128 Rat/mouse NS-1
TIB 166 Rat/mouse NS-1
TIB 168 Rat/mouse NS-1
RS Rat/mouse P3X63Ag 8.653
96
Classical Media
RPMI 1640 MediumRPMI is a general purpose media with a broad range of applications for mammalian cells, especially hematopoietic cells. RPMI with MOPS (04-525F) can be used with certain mycological assays.
Cat. No. Size Without L-GLutamine
Without Phenol Red
With HEPES With Penicillin Streptomycin
Without Na-Bicarbonate
With MOPS Buffer
With Ultra-Glutamine I
Without D-Glucose
12-702F 500 mL •
12-702Q 1 L •
BE12-702F/U1 500 mL •
12-167F 500 mL
12-167Q 1 L
BE12-115E 100 mL • •
12-115F/U1 500 mL • •
12-115F 500 mL • •
12-115Q 1 L • •
04-525F 500 mL • • •
BE04-558F 500 mL •
12-918F 500 mL •
09-774E 100 mL • • •
09-774F 500 mL • • •
BE12-752F 500 mL • •
Cat. No. NA Cat. No. EU Product Name Product Description Storage
Conditions
Size
BE12-702F/U1 RPMI 1640 With UltraGlutamine™ I 15°C to 30°C 500 mL
BE12-115F/U1 RPMI 1640 With UltraGlutamine™ I and 25 mM HEPES 15°C to 30°C 500 mL
04-525F 04-525F RPMI 1640 For certain mycological assays. With L-Glutamine and 165 mM MOPS, without sodium
bicarbonate
2°C to 8°C 500 mL
BE04-558F RPMI 1640 Without L-Glutamine, with 25mM HEPES 15°C to 30°C 500mL
09-774E 09-774E RPMI 1640 With L-Glutamine, 25 mM HEPES, 100 units/mL penicillin, and 50 μg/mL streptomycin 2°C to 8°C 100 mL
09-774F 09-774F RPMI 1640 With L-Glutamine, 25 mM HEPES, 100 units/mL penicillin, and 50 μg/mL streptomycin 2°C to 8°C 500 mL
12-115F BE12-115F RPMI 1640 With L-Glutamine and 25mM HEPES 2°C to 8°C 500 mL
12-115Q 12-115Q RPMI 1640 With L-Glutamine and 25mM HEPES 2°C to 8°C 1 L
12-167F BE12-167F RPMI 1640 Without L-Glutamine 15°C to 30°C 500 mL
12-167Q 12-167Q RPMI 1640 Without L-Glutamine 15°C to 30°C 1 L
12-702F BE12-702F RPMI 1640 With L-Glutamine 2°C to 8°C 500 mL
12-702Q 12-702Q RPMI 1640 With L-Glutamine 2°C to 8°C 1 L
12-918F BE12-918F RPMI 1640 Without L-Glutamine or phenol red 15°C to 30°C 500 mL
15-702D 15-702D RPMI 1640 With L-Glutamine 2°C to 8°C 10 L
BE12-752F BE12-752F RPMI 1640 With L-Glutamine, without D-glucose 2°C to 8°C 500 mL
Ordering Information
Features of the standard product
97
Classical Media
Cat. No. NA Cat. No. EU Product Name Product Description Storage Conditions Size
12-923E 12-923E NCTC-109 Medium With Earle‘s BSS and L-Glutamine, hybridoma screened 2°C to 8°C 100 mL
Cat. No. NA Cat. No. EU Product Name Product Description Size
BE02-019F BE02-019F William‘s Medium E Without L-Glutamine or phenol red 500 mL
BE12-761F William‘s Medium E Without L-Glutamine 500 mL
NCTC-109 MediumA complex formula used to supplement hybridoma medium.
William’s Medium E
Richter’s CM MediumA complex formula used to supplement hybridoma medium
Cat. No. NA Cat. No. EU Product Name Product Description Size
04-648F 04-648F Richter’s CM Medium With L-Glutamine 500 mL
Ordering Information
Ordering Information
Ordering Information
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Storage 2ºC-8ºC
Features of the standard product
Features of the standard product
Features of the standard product
98
Cat. No. NA Cat. No. EU Product Name Product Description Size
12-743F BE12-743F UltraMEM™ Reduced Serum Medium – Chemically Defined Low protein medium with ITES and L-Glutamine 500 mL
UltraMEM™ Reduced Serum MediaChemically Defined, General Purpose Serum-free Media
UltraMEM™ Reduced Serum Media – Chemically Defined is designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations (see table). When supplemented with 2–4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages.
Growth and maintenance of anchorage-dependent cell types
Applications
Reduced Serum Medium – Chemically Defined
Cell Type Recommended % Serum
Diploid
CEF, chicken fibroblast 2% FBS
WI-38, human fibroblast 3% FBS
RHMK, monkey kidney 2–4% FBS
MRC-5, human fibroblast 3% FBS
RK, rabbit kidney 3% FBS
BGMK, monkey kidney 2–3% FBS
HEL, human fibroblast 2–3% FBS
CMK, monkey kidney 3–4% FBS
Heteroploid
VERO, monkey kidney 3% FBS
CRFK, cat kidney 2–3% FBS
L929, mouse fibroblast 4% FBS
MDCK, dog kidney 2–3% FBS
McCOY, mouse fibroblast 4% FBS
3T3, mouse fibroblast 3–4% FBS
CHO-K1, hamster ovary 3% FBS
PK-15, pig kidney 3–4% FBS
MK2, monkey kidney 3–4% FBS
Ordering Information
Classical Media
Partial List of Cell Cultures Cultivated with UltraMEM™
Storage 2ºC-8ºC
Features of the standard productIn addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all. Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, and selenium to the basal medium UltraMEM™ Reduced Serum Medium, offered as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/mL.
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Related ProductsSolutions for Media Preparation
p.100
Sartopore® 2 XL Filter Family
p.102
Sartogard PreFilter Familyy
p.103
Virosart® p.104
UVivatec® p.105
Flexel® for Mixing1 p.106
FlexAct® MP p.107
Which Bioreactor Fits your Needs?
p.108
Cell culture media are produced in large volumes and aseptically transferred into the bioreactor. Key process steps involved in media preparation are the mixing of the powder media with water for injection (WFI), the sterilizing-grade and mycoplasmaretentive filtration and the media storage before use. Continuous cost pressure, increased titers and reduced production volumes have led to the adoption of single-use technologies – both for bioreactors and media preparation.
Sartorius has therefore developed the fully automated, easy-to-use FlexAct® MP system that helps to reduce manpower even further. As your leading partner for upstream processing, Sartorius you with integrated solutions that comprise cell culture media, bags, sterilizing grade filters, mycoplasma retentive filters and virus risk mitigation tools.
Mixing Bags
Sterilizing Grade Filters
Mycoplasma Retentive Filters
Virus Retention
Filters
UV Virus Inactivation
Storage Bags
Automated Media Preparation
Cell Culture Media
99
100
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Single-use Media Preparation
Today, single-use systems for media preparation are available up to 3.000 L volume. While users initially converted individual production components to single-use, the industry is now moving to completely integrated systems for media preparation. Sartorius FlexAct® MP media preparation platform is a highly automated, fully closed system: It starts with powder dissolution in a single-use mixing system followed by sterile filtration and the transfer of the ready-to-use media into a storage bag.
Virus Risk Mitigation in Media Preparation
Virus risk mitigation in cell culture media preparation is a hot topic right now. Almost all product contaminations during the past several years were caused by small, nonenveloped viruses derived from raw materials. As a result, virus removal technologies that are effective for small, non-enveloped viruses gain importance in upstream processing. Technologies such as high Temp short time treatment HTST (Hight Temperature Short Time), UV-C inactivation as well as virus filtration have been employed for virus risk mitigation in cell culture media preparation. UV-C inactivation is also applicable for virus inactivation of serum and serum-containing media. Virosart filters have been successfully applied at low flow rates for chemically defined media filtration in continuous perfusion processes.– Broad range of virus filters for different media filtration applications.– Safe virus inactivation in serum and serum containing media by UV-C inactivation.– Orthogonal and robust technologies prevent contaminations.
Powder Media Mixing Filtration Storage› › ›
Solutions for Media Preparation
In order to avoid errors, the entire media preparation process is supervised by a multifunctional central control unit. It monitors and records all relevant process data, such as operating pressure, pH, pump speed and fluid level. Sartorius filters and single-use bags are supported by an industry-leading documentation package, including validation and extractables guides. Our validation experts support the customers during qualification of single-use systems for biopharmaceutical production.
– Complete range of single-use bags for media preparation, mixing and storage from 50 L up to 3.000 L volume for scale-up from R & D to production with proven performance.
– Magnetic mixing technology with universal mobile drive unit
– Fully integrated media preparation platform FlexAct® MP.
– Comprehensive validation support for extractables | leachables by dedicated CONFIDENCE validation team.
Media Filtration Solutions
The choice of an appropriate filtration solution for media preparation highly depends on the composition of the cell culture medium. Often 0.2 μm-rated sterilizing-grade filters are used for media preparation. However, reports of mycoplasma contamination of cell culture processes have put 0.1 μm-rated mycoplasma-retentive filters into the spotlight, again. Typical sources for mycoplasma contamination are animal-derived materials such as trypsin or serum, plant-derived culture media supplements such as soy-based supplements as well as contaminations caused by the operators themselves. Producers of cell culture media have to assess their potential risk for mycoplasma contamination in order to decide which filtration method to use. Sartorius media filtration solutions range from prefilters to sterilizing grade and mycoplasma-retentive filters – tailored to the specific filtration needs of the various types of cell culture media.
101
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Serum, soy oryeast hydrolysatesupplementedcell culture media
Chemicallydefinedcell culturemedia
Serum orhydrolysatesupplementedmedia
Complex additives withbroad molecular weightdistribution and potentialkolloid and particle load
Might contain rec. proteinadditives such as insulin,transferin or othergrowth factors
Potential mycoplasmacontamination risk
Sartoguard GFGlas fibre fleece0.2 μm | 0.2 μm
Sartopore® 2XLG0.8 μm | 0.2 μm
Sartoguard NFNanofleece0.2 μm | 0.2 μm
Sartopore® 2XLG0.8 μm | 0.2 μm
Sartoguard PES0.2 μm | 0.1 μm
Sartopore® 2XLM0.2 μm | 0.1 μm
Media Type
Characteristics
Prefilter Options
Final Filter Options
Which Filter Solution Best Fits Your Needs?Today’s industrial cell culture media formulations can be very different, from serum and hydrolyzate supplemented to chemically defined media. Based on our long standing experience in media filtration, we can recommend the best filter solution for your needs. Our international team of application specialists can help you optimize your specific filtration process – just contact us today.
Sartorius offers:
– A broad portfolio of final membrane filters for various media types, including chemically defined or protein, serum or hydrolysate supplemented with highest total throughput and economic filtration system design.
– Secure solutions for mycoplasma risk mitigation combining high retention efficiency with optimal throughput for economic processing.
– A refilter portfolio with optimal protection of final filters to further reduce filtration costs– All media filtration solutions are available for single use processing or media production in stainless steel equipment.
102
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Sartopore® 2 XL Filter FamilyBest Filtration Solutions for Cell Culture Media
The Sartopore® 2 XL filter family is the best choice for sterilizing-grade filtration and mycoplasma-retentive filtration of cell culture media.
The unique range of PES double-layer membrane combinations perfectly serves the different cell culture media requirements. Sartopore® 2 XLM filters use double-layer PES membranes with pore size combination of 0.2 | 0.1 μm for reliable mycoplasma retention with an LRV of 7/cm2 filtration area.
They have additionally been validated for sterilizing grade filtration according to ASTM F 838-05. Sartopore. XL ensures unmatched filtration performance along with the highest microbial safety.
Unique combination of different pre- and final filter membranes
k Ensures highly economic filter sizing and minimum filtration costs for all kind of media
Low unspecific protein binding k Excellent and reproducible cell growth
Reliable retention of mycoplasma by Sartopore 2XLM 0.1μm k Prevents mycoplasma contamination of your cell culture process
Available in a broad range of sizes and formats to provide linear scale-up from development to commercial-scale production
k Identical filtration performance at all scale-up levels of your media preparation process
Applications
– Sterilizing-grade filtration and mycoplasma-retentive filtration of cell culture media
– Sartopore 2 XLG (08 | 0.2 μm) designed for complex. serum or hydrolyzate supplemented media and for chemically defined media
– Sartopore. 2 XLM (0.2 | 0.1 μm) designed for mycoplasma retention (0.1 μm)
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Sartoguard Prefilter FamilyOptimal Protection for Sartopore® 2 XL Filter Elements
The Sartoguard family consisting of PES, GF and NF versions are all designed to extend the lifetime of Sartopore® 2 XL final filters by a factor of 3 to 4 – for cost-effective cell culture media prefiltration. Sartoguard filters provide the most cost-effective solutions to ensure your final filter achieves the highest throughput. Sartoguard PES contains a PES double layer, available in both 0.1 and 0.2 μm nominal pore size configurations. They are designed for cost-effective prefiltration of chemically defined media.Sartoguard NF is a gamma-irradiatable prefilter which contains a PES double layer covered with a modern PES naNofleece material. Sartoguard GF contains a PES double layer covered with a glass fiber fleece. It effectively protects the filter from clogging, especially when you need to filter serum and hydrolysate supplemented cell culture media.
Heterogeneous PES membrane double layer with or without fleece material− provides optimal prefiltration for all types of
cell culture media− protects subsequent sterile filters and extend
filtration capacity
Sartoguard prefilter family k Protects subsequent sterile filters and extend filtration capacity
Sartoguard PES k For prefiltration of chemically defined media
Sartoguard NF k For prefiltration of serum-free media with higher tendency of clogging final filter
Staroguard GF k For prefiltration of complex media containing serum of hydrolysates
– Prefiltration of cell culture media– Protection of Sartopore XL final filters
Applications
104
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Virosart®
Optimal Protection for Sartopore® 2 XL Filter Elements
Virus-retentive filtration is a highly efficient method for viral risk mitigation of cell culture media as these filters target both Non-enveloped viruses, such as MVM and PPV, and large enveloped viruses, such as MLV. Sartorius’ robust virus removal technology has been proven throughout the industry as providing the highest safety for your cell culture without impacting their performance.
You can choose from three virus-retentive membranes to suit your specific media composition, e.g., hydrolysate and protein content.
Unlike most other virus retentive filters, all Sartorius virus-retentive filters reliably and robustly provide > 4 LRV for small non-enveloped viruses over the entire flow decay profile.
Robust performance over a wide range of flow rates and process conditions
k The highest possible safety
Sterile and gamma-irradiated virus removal filter set-ups (e.g. Virosart® HF)
k – Ready-to-use– No need for preflushing
High-performance PES membranes k – Capacity from 1,000 to 10,000 L/m2 achieved for in-line virus-retentive filtration in perfusion cultures
– No impact on cell culture performance
Integrity testing with Sartocheck® 4 Plus Filter tester (based on diffusion)
k Easy, safe, fast, cost-effective
– Viral risk mitigation of chemically defined media– Viral risk mitigation of perfusion media feeds– Sterile media– Low protein and hydrolysate content cell culture media
Applications
105
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UVivatec®
Virus Inactivation Based on UV-C Irradiation
Virus inactivation by UV-C irradiation is a highly efficient technology that specifically targets small, non-enveloped viruses (e.g., PPV, MVM) at low doses.
Difficult-to-filter and heat-instable media components like serum can be safely inactivated by UV-C (254 nm) without impacting cell growth or cell viability.
Even highly turbid media or serum can be easily exposed to the UV source without any risk of blocking the flow path. Small, non-enveloped viruses like MVM show good clearance of six LRV already at low doses of around 100–150 J/m2 while maintaining cell growth performance.
– Serum virus inactivation– Virus inactivation of serumcontaining media– Virus inactivation of heat sensitive components
Inactivation based on UV-C (254 nm) irradiation
k Especially effective for small, non-enveloped viruses (e.g., MVM)
Novel helical module with optimized hydrodynamic design
k – Uniform irradiation through efficient radial mixing based on Dean vortices– Narrow residence time distribution– Effective inactivation at low doses
Single-use module concept k – Quick and easy to install and maintain– Easy to scale
Applications
106
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Flexel® for Mixing1Single-Use Media Mixing Solution
Flexel® for Magnetic Mixer® 1 is a compact and non-invasive single-use mixing system for manual media dissolution and mixing.The system is based on magnetic coupling, e.g., no shaft-through or dynamic seal is used, providing a completely closed mixing assembly.Agitation rates of up to 300 rpm can be reached, allowing highly efficient mixing from 50 – 3,000 L scale.
Flexel® for Magnetic Mixer. 1 with its strong magnetic coupling is the ideal solution for:− Powerful dissolution of powder media with high
torque and high rotation speed− Effective mixing of highly viscous solutions,
such as media and hydrolysate mixtures− Large volumes (up to 3,000 L)
– Media | feed preparation– Buffer preparation
Cubical shape with front access system and large 8” diameter top port k Bag installation, filling, powder transfer and draining steps easy to perform
Baffle-like effect with strong top to bottom flow pattern thanks to the centered impeller in combination with the cubical design
k High performance proven from 50-3,000 L
Pre-assembled and presterilized single-use sensors for pH, conductivity and temperature
k In-line monitoring and control of critical process parameters
Palletank® for mixing with heat exchange jacket and integrated weighing functions
k Product temperature and volume control
Dust-free powder addition via closed addition bag k Protection of operators, meeting current health and safety regulations
1 This product uses Pall patented Magnetic Mixer technology.All information on patents can be found at Pall.com/patents.
Applications
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FlexAct® MPConfigurable Single-Use Solution for Media Preparation
The FlexAct® MP is a standardized configurable system for convenient media preparation in biopharmaceutical processes. The FlexAct® central operation module enables you to control and monitor all relevant process parameters during media preparation.Integrated weighing capabilities permit precise media dissolution using our Magnetic Mixer®1 technology. A fully automated, temperature-controlled pH adjustment is performed via a jacketed Palletank® for mixing and a temperature control unit (TCU).During fluid transfer and sterile filtration, pressure and flow are monitored and controlled to ensure safe operation.
Multi-functional central operating module k Operator-friendly
Tailored bag configuration k Flexible media supply
50-3,000 L working volume k Fully scalable
Quick system set-up k Increased efficiency
Integrated disposable sensors (pH, conductivity, temperature) k Reliable monitoring of important parameters
Bi-directional operation k Highly flexible
1 MagMixer® is a trademark of Pall Corporation and this product uses Pall patented MagMixer® technology.All information on patents can be found at Pall.com/patents.
– Automatic, contained media preparationApplications
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Which Bioreactor Fits your Needs?
Available for Cell Culture (CC) or Microbial (MO)
Culture
Screening ofMedia, Clonesor ExpressionConstructs
Small ScaleProteinSupply
ProcessDevelopmentOptimization,Characteri-
sation
Seed Expansion Production Product Product
Vessel TypeNumber
of ParallelVessels
Max. VesselWorking VolumeSingle-Use Glass Stainless Steel
CC ☐ (☐)ambr® 15 ambr® 15
h24 | 48 10 – 15 mL
MO
CC ☐ (☐) ☐ ambr® 250 ambr® 250
h12 | 12 100 – 250 mL
MO ☐ (☐) ☐ h
CC ☐ ☐ (☐) ☐CERTOMAT® CTplus CERTOMAT® CTplus
h6 | 98 12.5 mL – 2.5 L
MO
CC (☐) ☐ (☐) ☐BIOSTAT® A BIOSTAT® A
h h1 1 – 5 L
MO (☐) ☐ (☐) ☐ h
CC ☐ ☐ ☐ ☐BIOSTAT® B BIOSTAT® B
h h2 1 – 10 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ ☐ ☐BIOSTAT® B-DCU II BIOSTAT® B-DCU II
h h6 0.5 – 10 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ ☐ (☐)BIOSTAT® Cplus BIOSTAT® Cplus
h1 5 – 30 L
MO (☐) ☐ ☐ ☐ (☐) h
CC ☐ ☐ ☐ ☐BIOSTAT® D-DCU BIOSTAT® D-DCU
h2 10 – 200 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ Customized Stainless Steel Fermenters
Customized Stainless Steel Fermenters
hmultiple 200 – 2,000 L
MO ☐ ☐ h
CC ☐ ☐ (☐)BIOSTAT® RM BIOSTAT® RM
h2 0.5 – 100 L
MO (☐) (☐) (☐) h*
CC ☐ ☐ ☐ ☐BIOSTAT STR® BIOSTAT STR®
✓2 50 – 2,000 L
MO (☐) (☐) (☐) (☐) ✓*
☐ Typical Applications(☐) Selected Applications
ambr® 15 ambr® 250 CERTOMAT® CTplus Biostat® A Biostat® B
109
Related Products
Available for Cell Culture (CC) or Microbial (MO)
Culture
Screening ofMedia, Clonesor ExpressionConstructs
Small ScaleProteinSupply
ProcessDevelopmentOptimization,Characteri-
sation
Seed Expansion Production Product Product
Vessel TypeNumber
of ParallelVessels
Max. VesselWorking VolumeSingle-Use Glass Stainless Steel
CC ☐ (☐)ambr® 15 ambr® 15
h24 | 48 10 – 15 mL
MO
CC ☐ (☐) ☐ ambr® 250 ambr® 250
h12 | 12 100 – 250 mL
MO ☐ (☐) ☐ h
CC ☐ ☐ (☐) ☐CERTOMAT® CTplus CERTOMAT® CTplus
h6 | 98 12.5 mL – 2.5 L
MO
CC (☐) ☐ (☐) ☐BIOSTAT® A BIOSTAT® A
h h1 1 – 5 L
MO (☐) ☐ (☐) ☐ h
CC ☐ ☐ ☐ ☐BIOSTAT® B BIOSTAT® B
h h2 1 – 10 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ ☐ ☐BIOSTAT® B-DCU II BIOSTAT® B-DCU II
h h6 0.5 – 10 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ ☐ (☐)BIOSTAT® Cplus BIOSTAT® Cplus
h1 5 – 30 L
MO (☐) ☐ ☐ ☐ (☐) h
CC ☐ ☐ ☐ ☐BIOSTAT® D-DCU BIOSTAT® D-DCU
h2 10 – 200 L
MO ☐ ☐ ☐ ☐ h
CC ☐ ☐ Customized Stainless Steel Fermenters
Customized Stainless Steel Fermenters
hmultiple 200 – 2,000 L
MO ☐ ☐ h
CC ☐ ☐ (☐)BIOSTAT® RM BIOSTAT® RM
h2 0.5 – 100 L
MO (☐) (☐) (☐) h*
CC ☐ ☐ ☐ ☐BIOSTAT STR® BIOSTAT STR®
✓2 50 – 2,000 L
MO (☐) (☐) (☐) (☐) ✓*
✓ Available✓* Available for low cell density applications
BIOSTAT® B-DCU II BIOSTAT® Cplus BIOSTAT® D-DCUCustomized Stainless Steel
FermentersBIOSTAT® RM BIOSTAT STR®
110
AO Animal or human Origin
BHK Baby Hamster Kidney
BME Basal Medium Eagle
CD Chemically Defined
cGMP Current Good Manufacturing Practices
CHO Chinese Hamster Ovary
CoA | C of A Certificate of Analysis
CRO Contract Research Organization
CTM Clinical Trial Management
DF Dia Filtration
DMEM Dulbecco's Modified Eagle Medium
DOE Design of Experiment
DPBS Dulbecco's Phosphate Buffered Saline
DSP Down Stream Processing
EMA European Medicine Agency
ESC Embryonic Stem Cell
F&F Fill and Finish
FBS Fetal Bovine Serum
FDA Food and Drug Agency
FGM Fibroblast Growth Medium
FMU For Further Manufacturing Use
FTM Fluid Thioglycollate Medium
GMEM Glasgow Minimum Essential Medium
HEK Human Embryonic Kidney
hPSC Human Pluripotent Stem Cell
Glossary
Comprehensive Glossary for All Abbreviations
111
Glossary
IMDM Iscove's Modified Dulbecco's Medium
iPSC Induced Pluripotent Stem Cell
KGM Keratinocyte Growth Medium
LAK Lymphocyte Activated Killer
MDCK Madin Carby Canine Kidney
MEM Minimal Essential Medium
MSCBM Mesenchymal Stem Cell Basal Medium
MSCGM Mesenchymal Stem Cell Growth Medium
NAO Non-Animal and non-human Origin
NK Natural Killer
PBL Peripheral Blood Lymphocyte
PBS Phosphate Buffered Saline
PDT Population Doubling Time
PF* Protein Free
R&D Research and Development
RUO Research Use Only
Sf Spodoptera frugiperda
SSB Sartorius Stedim Biotech
TIL Tumor Infiltrating Lymphocyte
TSB Trypticase Soy Broth
UF Ultra Filtration
USP Up Stream Processing
USP | EP US Pharmacopeia | European Pharmacopeia
WFI Water for Injection
*human recombinant insulin is NOT considered a protein, both because it is fully characterized, and because of its size (<5kDa)
112
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
BE12-730P10 BE12-730P10 STD Insect-XPRESS w/ L-Gln, 10 L bag 10 L 1) Multi Purpose Media
BP12-730Q BEBP12-730Q Insect-XPRESS w/ L-Gln 1 L 1 L 1) Multi Purpose Media
BP12-730F BEBP12-730F Insect-XPRESS w/ L-Gln, 500 mL 500 mL 1) Multi Purpose Media
BP12-725F BEBP12-725F UltraCULTURE serum-free w/o L-Gln 500 mL 500 mL 1) Multi Purpose Media
04-501Q 04-501Q Grace’s Insect Medium (TNM-FH) w/FBS 1 L 1 L 1) Multi Purpose Media
04-649F 04-649F Grace’s Insect Medium w/o FBS, 500 mL 500 mL 1) Multi Purpose Media
04-457F 04-457F Grace’s Insect w/o FBS, Gent. LAH, 500 mL 500 mL 1) Multi Purpose Media
04-351Q 04-351Q Schneider’s Drosophila Medium, Mod., 1 L 1 L 1) Multi Purpose Media
N/A BE02-011F TC100 Insect Medium 500 mL 500 mL 1) Multi Purpose Media
02-044Q BE02-044Q STD PowerFeed A, 1 L bottle 1 L 2) Proteins
02-059F BE02-059F STD FeCitrate100x-PowerCHO™-2KIT, 500 mL bt 500 mL 2) Proteins
02-059Q BE02-059Q STD FeCitrate100x-PowerCHO™-2KIT, 1 L bt 1 L 2) Proteins
12-771P10 BE12-771P10 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 10 L 10 L 2) Proteins
12-771P20 BE12-771P20 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 20 L 20 L 2) Proteins
15-044D BE15-044D STD PowerFeed A Powder kit 10 L 10 L 2) Proteins
15-044F BE15-044F STD PowerFeed A Powder kit 50 L 50 L 2) Proteins
15-044J BE15-044J STD PowerFeed A Powder kit 100 L 100 L 2) Proteins
15-044Q BE15-044Q STD PowerFeed A Powder kit 200 L 200 L 2) Proteins
15-766D BE15-766D STD ProCHO5 Powder kit w/o PR 10 L 10 L 2) Proteins
15-766F BE15-766F STD ProCHO5 Powder kit w/o PR 50 L 50 L 2) Proteins
15-766L BE15-766L STD ProCHO5 Powder kit w/o PR 500 L 500 L 2) Proteins
15-771ND BE15-771ND STD PowerCHO™2 Powder kit 10 L 10 L 2) Proteins
15-771NF BE15-771NF STD PowerCHO™2 Powder kit 50 L 50 L 2) Proteins
15-771NJ BE15-771NJ STD PowerCHO™2 Powder kit 100 L 100 L 2) Proteins
12-927Q 12-927Q STD PowerCHO™ Advance™ 1 L bt 1 L 2) Proteins
BE02-043E BE02-043E STD FeCitrate1000x PowerCHO™-GS kit, 100 mL 100 mL 2) Proteins
BEBP02-052Q BEBP02-052Q STD Power Feed A with Lipids – 1 L bottle 1 L 2) Proteins
BE02-056Q BE02-056Q STD CHO Xtreme Feed™ CD – 1 L bottle 1 L 2) Proteins
BE12-766P10 BE12-766P10 STD ProCHO5w/Pluronicw/oL-Gln, HT, 10 L 10 L 2) Proteins
BE12-766P20 BE12-766P20 STD ProCHO5w/Pluronicw/o L-Gln, HT, 20 L 20 L 2) Proteins
BE15-771D BE15-771D STD PowerCHO™2 Powder kit 10 L 10 L 2) Proteins
BE15-771F BE15-771F STD PowerCHO™2 Powder kit 50 L 50 L 2) Proteins
BE15-771J BE15-771J STD PowerCHO™2 Powder kit 100 L 100 L 2) Proteins
BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L 2) Proteins
N/A BE15-770F STD PowerCHO™1 Powder kit 50 L 50 L 2) Proteins
N/A BE15-771L STD PowerCHO™2 Powder kit 500 L 500 L 2) Proteins
N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L 2) Proteins
BP12-770Q BP12-770Q PowerCHO™-1 serum-free medium 1 L 1 L 2) Proteins
BEBP17-855E BEBP17-855E ProHT Supplement 100 × 100 mL 100 mL 2) Proteins
BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1L bottle 1 L 2) Proteins
N/A BEBP02-042Q PowerCHO™-2 CD, w/o glucose, 1 L 1 L 2) Proteins
BP12-771Q BEBP12-771Q PowerCHO™-2 CD, w/o L-Gln w/o PR or HT, 1 L 1 L 2) Proteins
113
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
BP12-772Q BEBP12-772Q PowerCHO™-3 CD, w/o L-Gln w/o PR or HT, 1 L 1 L 2) Proteins
BP04-919Q BP04-919Q ProCHO 4 w/o Phenol Red, 1 L 1 L 2) Proteins
BP12-029Q BEBP12-029Q ProCHO4 w/o L-Gln, HT 1 L 1 L 2) Proteins
N/A BEBP02-041Q ProCHO5 w/o glucose, 1 L 1 L 2) Proteins
BP12-766Q BEBP12-766Q ProCHO5 w/Pluronic w/o L-Gln or HT, 1 L 1 L 2) Proteins
BEBP02-016Q BEBP02-016Q PROCHO-AT w/L-Gln 1L, w/o HT 1 L 2) Proteins
BEBP02-029Q BEBP02-029Q ProDoma 1 serum-free medium, 1 L 1 L 2) Proteins
BEBP02-032Q BEBP02-032Q ProDoma 3 woL-gln,PR w 0.1% Pluro F68 1L 1 L 2) Proteins
BP12-724Q BP12-724Q UltraCHO w/L-Gln 1 L 1 L 2) Proteins
N/A BEBP12-723F UltraDOMA serum-free w/o L-Gln 500 mL 500 mL 2) Proteins
BP12-723B BP12-723B UltraDOMA SF hybridoma medium, 500 mL 500 mL 2) Proteins
BP12-727F BP12-727F UltraDOMA-PF Hybridoma Medium, 500 mL 500 mL 2) Proteins
BESP904 BESP904 Lonza medium base 1 20,18 g 2) Proteins
BESP905 BESP905 Feed Base K 10,23 g 2) Proteins
BESP906 BESP906 Feed Base L 28,7 g 2) Proteins
BESP907 BESP907 Feed Base M 2,36 g 2) Proteins
BESP908 BESP908 Feed Base N 0,18 g 2) Proteins
BESP799 BESP799 Feed base F powder 1,75 g 2) Proteins
BESP862 BESP862 Feed base E powder 1,72 g 2) Proteins
BESP808 BESP808 CHO Vitamin Mix 0,18 g 2) Proteins
BESP794 BESP794 Feed Base A 43,06 g 2) Proteins
BESP795 BESP795 Feed Base B 112,2 g 2) Proteins
BESP734 BESP734 NMB1 22,04 g 2) Proteins
BESP735 BESP735 NMB2 18,34 g 2) Proteins
BESP1023 BESP1023 Feed Base R 114,52 g 2) Proteins
BESP876 BESP876 Feed Base 2 6,47 g 2) Proteins
BESP887 BESP887 MEM Vitamin Powder 9,31 g 2) Proteins
BESP899 BESP899 Feed Base G 42,63 g 2) Proteins
BESP900 BESP900 Feed Base H 69,04 g 2) Proteins
BESP901 BESP901 Feed Base J 133,87 g 2) Proteins
BESP1021 BESP1021 LMdB2 19,97 g 2) Proteins
BESP1022 BESP1022 Feed Base P 72,17 g 2) Proteins
BESP797 BESP797 Feed Base D 51,88 g 2) Proteins
BESP531E BESP531E SPE 100 mL 2) Proteins
BESP531F BESP531F SPE 500 mL 2) Proteins
BESP531Q BESP531Q SPE 1000 mL 2) Proteins
BESP909E BESP909E LTEM ONE 100 mL 2) Proteins
BESP909Q BESP909Q LTEM ONE 1000 mL 2) Proteins
BESP910E BESP910E LTEM TWO 100 mL 2) Proteins
BESP910Q BESP910Q LTEM TWO 1000 mL 2) Proteins
BESP969E50 BESP969E50 SP6 50 mL 2) Proteins
BESP969F BESP969F SP6 500 mL 2) Proteins
BESP970E50 BESP970E50 SP7 50 mL 2) Proteins
114
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
BESP970F BESP970F SP7 500 mL 2) Proteins
BESP971E20 BESP971E20 SP8 20 mL 2) Proteins
BESP971E BESP971E SP8 100 mL 2) Proteins
BESP972E50 BESP972E50 SP9 50 mL 2) Proteins
BESP972F BESP972F SP9 500 mL 2) Proteins
BESP807E BESP807E CHO Trace Element Stock Solution 100 mL 2) Proteins
BESP807Q BESP807Q CHO Trace Element Stock Solution 500 mL 2) Proteins
12-924Q N/A ProMDCK (2D) 1 L 3)Vaccines
12-925Q N/A ProMDCK (3D) 1 L 3)Vaccines
BE02-039Q BE02-039Q GLS PERMEXCIS® virus production med, 1 L 1 L 3)Vaccines
BEBP02-028Q BEBP02-028Q ProPer-1 wo L-gln,PR w 0.1% Pluro F68 1 L 1 L 3)Vaccines
BP12-764Q BEBP12-764Q Pro293A-CDM, for adherent cells, 1 L 1 L 3)Vaccines
N/A BEBP02-025Q Pro293s-CDM, 1 L bottle 1 L 3)Vaccines
BP12-765Q BP12-765Q Pro293S-CDM, for suspension cells, 1 L 1 L 3)Vaccines
BEBP02-030Q BEBP02-030Q ProVero-1 NAO Medium for Vero Cells, 1 L 1 L 3)Vaccines
BP12-749Q BEBP12-749Q UltraMDCK serum-free 1 L 1 L 3)Vaccines
08-879H 08-879H STD X-Vivo 15 w/o gentamicin and pr, 5L 5 L 4) Regenerative Medicine
BEBP04-743Q BEBP04-743Q X-VIVO 10 w/o Gentamycin w/o PR 1L 1 L 4) Regenerative Medicine
BP04-380Q BEBP04-380Q X-Vivo 10 w/ Gentamycin and PR, 1L 1 L 4) Regenerative Medicine
BP04-418Q BEBP04-418Q X-Vivo 15 w/ Gentamycin and PR, 1 L 1 L 4) Regenerative Medicine
N/A BEBP04-418F X-Vivo 15 w/ Gentamycin and PR, 500 mL 500 mL 4) Regenerative Medicine
BP04-744Q BEBP04-744Q X-VIVO 15 w/o Gent or Phenol Red, 1 L 1 L 4) Regenerative Medicine
BP04-448Q BEBP04-448Q X-Vivo 20 w/ Gentamycin and PR, 1 L 1 L 4) Regenerative Medicine
BEBP02-055Q BEBP02-055Q X-VIVO10(04-743) contains rec transf -1 L 1 L 4) Regenerative Medicine
BEBP02-053Q BEBP02-053Q X-Vivo15(04-418) contains rec transf, 1 L 1 L 4) Regenerative Medicine
BEBP02-054Q BEBP02-054Q X-Vivo15(04-744) contains rec transf, 1 L 1 L 4) Regenerative Medicine
CC4455 CC4455 BulletKit KGM-CD Kit 4) Regenerative Medicine
CC-4456 CC-4456 BulletKit KGM-CD EA CC3255+CC4455 Kit 4) Regenerative Medicine
199019 199019 FBM-CD 4) Regenerative Medicine
199020 199020 FGM-CD SinglQuots kit Kit 4) Regenerative Medicine
190620 190620 MSCBM-CD™ 500 mL 500 mL 4) Regenerative Medicine
00199041 00199041 FBM-CD Bullet Kit Kit 4) Regenerative Medicine
190632 190632 MSCGM-CD bulletkit Kit 4) Regenerative Medicine
192125 192125 MSCGM-CD SingleQuots Kit Kit 4) Regenerative Medicine
CC-4126 CC-4126 FGM-2 SingleQuotKit Suppl&Growth Factors Kit 4) Regenerative Medicine
CC-3131 CC-3131 FBM Fibroblast Basal Medium, 500 mL 500ml 4) Regenerative Medicine
CC-3132 CC-3132 FGM-2 BulletKit (CC-3131 & CC-4126) Kit 4) Regenerative Medicine
04-1193E N/A L7™ hPSC Passaging Solution 100 mL 4) Regenerative Medicine
00215550 N/A L7™ hPSC Cryosolution 50 mL 4) Regenerative Medicine
00215549 N/A L7™ hPSC Matrix 1 mg 4) Regenerative Medicine
00215506 N/A L7™ hPSC Media BulletKit™ 500 mL Basal medium +
5 mL Supplement
4) Regenerative Medicine
115
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
04-1191F N/A L7™ hPSC Basal Medium 500 mL 4) Regenerative Medicine
04-1192J N/A L7™ hPSC Supplement 5ml 4) Regenerative Medicine
N/A BE02-038P200 Bulk Packaged sterile filtered WFI-200 L 200 L 5) Buffers
N/A BE15-512F STD DPBS Powder w/o Ca, Mg 50 L 50 L 5) Buffers
N/A BE15-512K STD DPBS Powder w/o Ca, Mg 1000 L 1000 L 5) Buffers
BE02-017F BE02-017F PBS-EDTA 500 mL 500 mL 5) Buffers
12-747F 12-747F UltraSaline A without Phenol Red, 500 mL 500 mL 5) Buffers
10-548E 10-548E ACK Lysing Buffer 100 mL 100 mL 5) Buffers
10-539B 10-539B Dextrose Gelatin Veronal Buffer, 500 mL 500 mL 5) Buffers
17-512Q/12 BE17-512Q/12 DPBS 0.0095M(PO4) w/o Ca and Mg, 12 × 1 L 12 × 1L 5) Buffers
17-512Q BE17-512Q DPBS 0.0095M(PO4) w/o Ca and Mg, 1 L 1 L 5) Buffers
17-512F BE17-512F DPBS 0.0095M(PO4) w/o Ca and Mg, 500 mL 500 mL 5) Buffers
17-512F/12 BE17-512F/12 DPBS 0.0095M(PO4) w/o Ca&Mg, 12 × 500 mL 12 × 500 mL 5) Buffers
17-515Q BE17-515Q DPBS-10x w/o Ca, Mg 1 L 1 L 5) Buffers
17-515F BE17-515F DPBS-10X w/o Ca, Mg 500 mL 500 mL 5) Buffers
04-479Q 04-479Q DPBS-1X w/ Ca & Mg Glucose and Pyr, 1 L 1 L 5) Buffers
17-513Q BE17-513Q DPBS-1X w/ Ca, Mg 1 L 1 L 5) Buffers
17-513F BE17-513F DPBS-1X w/ Ca, Mg 500 mL 500ml 5) Buffers
N/A BE17-513F/12 DPBS-1X w/ Ca, Mg BOX of 12 bottles 12 × 500 mL 5) Buffers
17-512F/24 17-512F/24 DPBS-1X w/o Ca++, Mg++ 24 × 500 mL 24 × 500 mL 5) Buffers
N/A BE02-027F EBSS w/ HEPES low NaHCO3, 500 mL 500 mL 5) Buffers
N/A BE10-502F EBSS w/ Phenol Red 500ml 500 mL 5) Buffers
10-527F/12 10-527F/12 HANKS’ BSS, W/O Phenol Red 12x500ml 12 × 500 mL 5) Buffers
10-508Q 10-508Q Hanks’ BSS, with Phenol Red, 1 L 1 L 5) Buffers
10-508F BE10-508F HBSS w/ Phenol Red 500ml 500 mL 5) Buffers
10-543F BE10-543F HBSS w/ PR w/o Ca, Mg 500ml 500 mL 5) Buffers
N/A BE10-543F/12 HBSS w/ PR w/o Ca, Mg BOX of 12 bottles 12 × 500 mL 5) Buffers
10-527F BE10-527F HBSS w/o Phenol Red 500 mL 500 mL 5) Buffers
10-547F BE10-547F HBSS w/o PR, Ca or Mg 500 mL 500 mL 5) Buffers
04-315Q 04-315Q HBSS, w/o Ca++,Mg++ or Phenol Red, 1 L 1 L 5) Buffers
10-543Q 10-543Q HBSS, w/o Ca++,Mg++ w/ phenol red, 1 L 1 L 5) Buffers
BE17-737E 17-737E HEPES Buf,1M Stk in normal saline, 100 mL 100 mL 5) Buffers
17-737F 17-737F HEPES Buffer,1M in normal saline, 500 mL 500 mL 5) Buffers
17-517Q BE17-517Q PBS-10x w/o Ca, Mg 1 L 1 L 5) Buffers
17-516Q/12 BE17-516Q/12 PBS-1X w/o Ca, Mg 12 × 1 L 12 ×1 L 5) Buffers
17-516F/12 BE17-516F/12 PBS-1X w/o Ca, Mg 12 × 500 mL 12 × 500 mL 5) Buffers
17-516Q BE17-516Q PBS-1X w/o Ca, Mg 1 L 1L 5) Buffers
17-516F BE17-516F PBS-1X w/o Ca, Mg 500 mL 500 mL 5) Buffers
17-516F/24 17-516F/24 PBS-1X, w/o Ca, Mg 24 × 500 mL 24 × 500 mL 5) Buffers
17-724Q BE17-724Q USP WFI bulk Sterile filtered 1 L 1 L 5) Buffers
17-724F BE17-724F USP WFI bulk Sterile filtered 500 mL 500 mL 5) Buffers
12-624E 12-624E Veronal Buffer (5X) 100 mL 100 mL 5) Buffers
116
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
N/A BE17-724F/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 500 mL 5) Buffers
N/A BE17-724Q/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 1 L 5) Buffers
02-033E BE02-033E STD Insulin Human recomb. 5 mg/ml 100 mL 100 mL 6) Reagents
02-033E20 BE02-033E20 STD rh Insulin, 5mg/ml, 20ml bottle 20 mL 6) Reagents
02-033Q N/A STD Human Recombinant Insulin 5mg/ml, 1 L 1 L 6) Reagents
13-668C BE13-668C STD Lipids 2x concentr. 35 mL 35 mL 6) Reagents
13-668Z BE13-668Z STD Lipids 2x concentr. 7 mL 7 mL 6) Reagents
15-605G BE15-605G L-GLUTAMINE POWDER 25 G 25 g 6) Reagents
15-613I BE15-613I STD Sodium Bicarbonate powder 500 g 500 g 6) Reagents
BE02-033E80 N/A Human Rec Insulin 5 mg/ml 80 mL 80 mL 6) Reagents
BE02-058E BE02-058E STD Anti clumping agent A 100 mL 100 mL 6) Reagents
BE13-668F BE13-668F STD Lipids 2x concentr. 500 mL 500 mL 6) Reagents
BE13-668Z BE13-668Z STD Lipids 2x concentr. 7 mL 7 mL 6) Reagents
N/A BE15-605X(3000) STD L-Glutamine Powder 3000 g 3000 g 6) Reagents
BP12-769E BP12-769E ProFreeze-CDM, NAO, 2x Freeze Med, 100 mL 100 mL 6) Reagents
BP12-773Q BP12-773Q ProNS0 1 CD 1 L 6) Reagents
BP12-775J BP12-775J ProNS0™ Lipid CD 5 mL 6) Reagents
BP12-774Q BP12-774Q ProNSO 2 CD 1 L 6) Reagents
17-905C 17-905C L-Glutamine 200mM Hybridoma test, 50 mL 50 mL 6) Reagents
17-160F 17-160F Trypsin(10x) 2.5% /HBSS w/o Ca,Mg,500 mL 500 mL 6) Reagents
BE02-007E BE02-007E Trypsin/EDTA 10 × 100 mL 100 mL 6) Reagents
N/A BE02-034E Trypzean/EDTA solution 100 mL bottle 100 mL 6) Reagents
BE17-605E/U1 BE17-605E/U1 Ultraglutamine I 200mM 100 mL 100 mL 6) Reagents
17-836R 17-836R Amphotericin B,antifungal 250 ug/ml,20 mL 20 mL 6) Reagents
17-836E 17-836E Amphotericin B,antifungal 250ug/ml,100 mL 100 mL 6) Reagents
12-132A 12-132A Cryoprotective Freezing Medium, 100 mL 100 mL 6) Reagents
17-519Z 17-519Z Gentamicin 10 mg/ml 1 × 10 mL screw cap 10 mL 6) Reagents
17-519L 17-519L Gentamicin 10 mg/ml 10 × 10 mL screw cap 10 × 10 mL 6) Reagents
17-528Z 17-528Z Gentamicin 50 mg/ml 1 × 10 mL crimp-top 10 mL 6) Reagents
17-518Z 17-518Z Gentamicin 50 mg/ml 1 × 10 mL screw cap 10 mL 6) Reagents
17-518L 17-518L Gentamicin 50 mg/ml 10 × 10 mL screw cap 10 × 10 mL 6) Reagents
N/A BE02-012E Gentamycin Sulfate 100 mL, 10 mg/ml Gent 100ml 6) Reagents
17-839Z 17-839Z ITES (500 × stock) 5 mL 5 mL 6) Reagents
17-838Z 17-838Z ITS (500 × stock) 5 mL 5 mL 6) Reagents
17-605E BE17-605E L-Glutamine 200mM 100 mL 100 mL 6) Reagents
17-605F BE17-605F L-Glutamine 200mM 500 mL 500 mL 6) Reagents
17-605C 17-605C L-Glutamine 200mM 50 mL 50 mL 6) Reagents
N/A BE17-605E/12 L-Glutamine 200mM BOX of 12 bottles 12x100ml 6) Reagents
17-602E DE17-602E Pen 10.000 UI/ml Strep 10.000 UI/ml 100ml 6) Reagents
17-602F DE17-602F Pen 10.000 UI/ml Strep 10.000 UI/ml 500ml 6) Reagents
17-603E DE17-603E Pen 5.000 U/ml Strep 5.000 U/ml 100ml 6) Reagents
09-757F 09-757F Pen/Strep stock, 20K/20K 500 mL 500ml 6) Reagents
17-745E 17-745E Pen/Strep/Fungizone 10K/10K/25ug, 100 mL 100ml 6) Reagents
117
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
17-745H 17-745H Pen/Strep/Fungizone 10K/10K/25ug, 20 mL 20ml 6) Reagents
17-718R 17-718R Pen/Strep/L-Gln 25K/25K/200mM, 25x4.5 mL 25x4.5ml 6) Reagents
N/A DE17-603E/12 Penicillin/Strep 5000U/ml BOX of 12 BOT 12x100ml 6) Reagents
N/A DE17-602E/12 Penicillin10.000 UI/ml BOX of 12 bottles 12x100ml 6) Reagents
17-613E BE17-613E Sodium Bicarb. 7,5% sol 100ml 100ml 6) Reagents
13-115E BE13-115E Sodium Pyruvate Solution 100 mM, 100ml 100ml 6) Reagents
17-942E 17-942E Trypan Blue 0.4% 0.85% NaCl,100ml 100ml 6) Reagents
17-160E BE17-160E Trypsin(10x)2.5% in HBSS w/o Ca, Mg100ml 100ml 6) Reagents
N/A BE17-161E/12 Trypsin-Versene (EDTA) 12 × 100ml 12x100ml 6) Reagents
17-161E BE17-161E Trypsin-Versene (EDTA) Mix (1X) 100ml 100ml 6) Reagents
17-161F BE17-161F Trypsin-Versene (EDTA) Mix (1X) 500ml 500ml 6) Reagents
N/A BE17-605E/U1/12 Ultraglutamine I 200mM BOX of 12 bottles 12x 100ml 6) Reagents
N/A BE04-684E UltraGlutamine II 100ml 100ml 6) Reagents
N/A BE04-684E/12 UltraGlutamine II BOX of 12 bottles 12x100ml 6) Reagents
17-711E BE17-711E Versene (EDTA) 0.02% 100ml 100ml 6) Reagents
08-012B N/A STD RPMI-1640 w/o L-Gln 10 L Bag 10L 7) Classical Media
08-143F N/A STD IMDM W/O L-GLUT, 20L bag 20L 7) Classical Media
15-604D BE15-604D DMEM 4.5 g/L Glucose Powder, w/Gln, 10L 10L 7) Classical Media
15-702D 15-702D STD RPMI 1640 w/L-Gln Powder, 10L 10L 7) Classical Media
BE15-604F BE15-604F STD DMEM high glucose w/ L-Glutamine 50L 50L 7) Classical Media
N/A 08-858Q STD TheraPEAK™ DMEM KIT for HS-12 7) Classical Media
N/A BE15-375D STD EMEM w/oL-Glut,NaHCO3,NaH2PO4,PR 10L 10L 7) Classical Media
N/A BE15-375F STD EMEM w/oL-Glut,NaHCO3,NaH2PO4,PR 50L 50L 7) Classical Media
N/A BE15-375J STD EMEMw/oL-Glut,NaH2PO4,NaHCO3,PR 100L 100L 7) Classical Media
N/A BE15-375J300 STD EMEMw/oL-Glut,NaHCO3,NaH2PO4,PR 300L 300L 7) Classical Media
N/A BE15-398D STD RPMI 1640 w/o PR NaHCO3, L-Glut 10L 10L 7) Classical Media
N/A BE15-398F STD RPMI 1640 w/o PR NaHCO3, L-Glut 50L 50L 7) Classical Media
N/A BE15-398K STD RPMI 1640 w/o PR NaHCO3,L-Glut 1000L 1000L 7) Classical Media
N/A BE15-399D STD DMEM powder 10L 10L 7) Classical Media
N/A BE15-399F STD DMEM Powder 50L 50L 7) Classical Media
N/A BE15-399J STD DMEM Powder 100L 100L 7) Classical Media
N/A BE15-408D STD IMDM Powder w/o HEPES, PR L-Glut 10L 10L 7) Classical Media
N/A BE15-408F STD IMDM Powder w/o HEPES, PR L-Glut 50L 50L 7) Classical Media
N/A BE15-408J STD IMDM Pwd w/o HEPES, PR L-Glut 100L 100L 7) Classical Media
N/A BE15-408Q STD IMDM Pwd w/o HEPES, PR L-Glut 200L 200L 7) Classical Media
N/A BE15-604D STD DMEM high glucose w/l L-Glu 10L 10L 7) Classical Media
N/A BE15-611D MEM Eagle powder w/ EBSS - 10L 10L 7) Classical Media
N/A BE15-611F MEM Eagle powder w/ EBSS - 50L 50L 7) Classical Media
N/A BE15-611K MEM Eagle powder w/ EBSS - 1000L 1000L 7) Classical Media
N/A BE15-611X MEM Eagle powder w/ EBSS - vrac 7) Classical Media
BP77227 BP77227 HL-1 chemically defined FBS subst. 10 mL 10ml 7) Classical Media
BP77201 BEBP77201 HL-1 Kit 2x BE344017 Kit 7) Classical Media
BP344022 N/A PC-1 Supp 10mL A Component of 77232 10ml 7) Classical Media
118
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
BP77232 BP77232 PC-1 w/o Gln 2x500 mL 2x344018; 2x344022 2x500ml 7) Classical Media
BP12-743F BEBP12-743F UltraMEM w/ ITES 500 mL 500ml 7) Classical Media
12-615Q 12-615Q Ham’s F-12 Medium w/ L-Gln, 1L 1L 7) Classical Media
BE02-002F BE02-002F Alpha MEM Eagle w/ UGln1 & nucleo,500 mL 500ml 7) Classical Media
BE12-604F/U1 BE12-604F/U1 DMEM 4.5g/L Glucose w/ UltraGlut I 500ml 500ml 7) Classical Media
BE04-687F/U1 BE04-687F/U1 DMEM:F12 1: 1 w/ UGln 1, 500ml 500ml 7) Classical Media
BE04-687Q BE04-687Q DMEM:F12 1:1 Mixture 1L 1L 7) Classical Media
BE12-668F BE12-668F EMEM (2x) w/o L-Glu, PR 500 mL bottle 500ml 7) Classical Media
12-736E 12-736E MEM Eagle EBSS, Maint., w/FBS+, 100 mL 100ml 7) Classical Media
N/A BE12-169F/12 Alpha MEM Eagle w/o L-Gln BOX of 12 BOT 12x500ml 7) Classical Media
12-169F BE12-169F Alpha MEM Eagle w/o L-Glutamine 500ml 500ml 7) Classical Media
12-105F BE12-105F BME w/ EBSS w/o L-Glutamine, 500ml 500ml 7) Classical Media
12-709F BE12-709F DMEM 4,5g/L Glu,25mM HEPE,w/oL-Gln 500ml 500ml 7) Classical Media
N/A BE12-709F/12 DMEM 4,5g/L Gluc,25mM BOX of 12 bottles 12x500ml 7) Classical Media
12-733Q 12-733Q DMEM 4.5 g/L Gluc w/o L-Gln or Pyr, 1 L 1L 7) Classical Media
12-604Q/12 12-604Q/12 DMEM 4.5 g/L Glucose w/ L-Gln, 12 × 1L 12x1L 7) Classical Media
12-604Q 12-604Q DMEM 4.5 g/L Glucose w/ L-Gln, 1L 1L 7) Classical Media
12-614Q 12-614Q DMEM 4.5 g/L Glucose w/o L-Gln, 1 L 1L 7) Classical Media
12-614Q/12 12-614Q/12 DMEM 4.5 g/L Glucose w/o L-Gln, 12 × 1 L 12x1L 7) Classical Media
12-604F/12 BE12-604F/12 DMEM 4.5g/L Glucose w/ L-Glut 12 × 500ml 12x500ml 7) Classical Media
12-604F BE12-604F DMEM 4.5g/L Glucose w/ L-Glutamine 500ml 500ml 7) Classical Media
N/A BE12-604F/U1/12 DMEM 4.5g/L Glucose w/ U1 BOX of 12 BOT 12x500ml 7) Classical Media
12-917F BE12-917F DMEM 4.5g/L Glucose w/o L-Gln, PR, 500ml 500ml 7) Classical Media
N/A BE12-741F/12 DMEM high glucose BOX of 12 bottles 12x500ml 7) Classical Media
12-741F BE12-741F DMEM high glucose w/o Na Pyruvate 500ml 500ml 7) Classical Media
12-708F BE12-708F DMEM w/ 1,0 g/l Gluc w/ 25mM Hepes,500ml 500ml 7) Classical Media
N/A BE12-708F/12 DMEM w/ 1,0 g/l Gluc,25mM Hepes,12x500ml 12x500ml 7) Classical Media
12-614F/12 BE12-614F/12 DMEM w/ 4.5g/L gluc w/o L-Glut 12x500ml 12x500ml 7) Classical Media
12-614F BE12-614F DMEM w/ 4.5g/L glucose w/o L-Glut 500ml 500ml 7) Classical Media
N/A BE12-733F/12 DMEM w/ 4.5g/L glucose,BOX of 12 bottles 12x500ml 7) Classical Media
N/A BE12-707F/12 DMEM w/1,0 g/L glucose BOX of 12 bottles 12x500ml 7) Classical Media
12-707F BE12-707F DMEM w/1,0 g/L glucose w/o L-Glut, 500ml 500ml 7) Classical Media
12-733F BE12-733F DMEM w/4.5g/L gluc w/o L-Gln, Na Pyruvat 500ml 7) Classical Media
N/A BE12-719F/12 DMEM:F-12 1:1 Mixture BOX of 12 bottles 12x500ml 7) Classical Media
12-719F BE12-719F DMEM:F-12 1:1 w/15mM Hepes, L-Gln 500ml 500ml 7) Classical Media
12-719Q 12-719Q DMEM:F12 1:1 with HEPES, L-Gln, 1 L 1L 7) Classical Media
12-914F BE12-914F DMEM4.5g/L Gluc w/oL-Gln Hybrid tst 500 mL 500 mL 7) Classical Media
12-668E 12-668E EMEM EBSS (2x), w/o Gln, Phen Red,100 mL 100 mL 7) Classical Media
12-662Q 12-662Q EMEM EBSS w/ NEAA, Pyr, w/o L-Gln, 1 L 1 L 7) Classical Media
12-739F BE12-739F Glasgow-MEM w/ L-Glutamine 500 mL 500 mL 7) Classical Media
12-618F BE12-618F Ham’s F-10 Medium w/ L-Glutamine 500 mL 500 mL 7) Classical Media
N/A BE02-014F Ham’s F10 w/ UGln I 500 mL, w/o Thymidine 500 mL 7) Classical Media
119
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
12-615F BE12-615F Ham’s F-12 w/ L-Glutamine 500 mL 500 mL 7) Classical Media
N/A BE12-615F/12 Ham’s F-12 w/ L-Glutamine BOX of 12 BOT 12 × 500 mL 7) Classical Media
N/A BE12-618F/12 Ham’sF-10 Medium w/ L-Gln BOX of 12 BOT 12 × 500 mL 7) Classical Media
12-722F BE12-722F IMDM w/ 25 mM HEPES 500 mL 500 mL 7) Classical Media
N/A BE12-722F/12 IMDM w/ 25 mM HEPES BOX of 12 bottles 12 × 500 mL 7) Classical Media
12-915F BE12-915F IMDM w/ L-Glutamine w/ 25mM HEPES 500 mL 500 mL 7) Classical Media
12-726F BE12-726F IMDM w/o L-Glut w/25 mM HEPES, 500 mL 500 mL 7) Classical Media
12-726F/12 BE12-726F/12 IMDM w/o L-Glutamine BOX of 12 bottles 12 × 500 mL 7) Classical Media
12-722Q 12-722Q IMDM with HEPES and L-Gln, 1 L 1 L 7) Classical Media
12-726Q/12 12-726Q/12 IMDM with HEPES w/o L-Gln 12 × 1 L 12 × 500 mL 7) Classical Media
12-726Q 12-726Q IMDM with HEPES w/o L-Gln, 1 L 1 L 7) Classical Media
12-700Q 12-700Q L-15 (Leibovitz), without L-Gln, 1 L 1 L 7) Classical Media
12-700F BE12-700F L-15 (Leibovitz), without L-Gln, 500 mL 500 mL 7) Classical Media
12-669E 12-669E L-15(2x) w/o L-Gln or Phenol Red, 100 mL 100 mL 7) Classical Media
N/A BE12-700F/12 L-15(Leibovitz) w/o L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media
N/A BE02-015E Lymphochrome w/L-Gln w/ Gent PHA-P 100 mL 100 mL 7) Classical Media
12-688F BE12-688F McCoy’s 5A Medium w/ L-Glutamine 500 mL 500 mL 7) Classical Media
12-168F BE12-168F McCoy’s 5A w/ Gln w/ 25mM Hepes 500 mL 500 mL 7) Classical Media
12-118F BE12-118F Med 199 w/ HBSS,L-Gln, w/25mM Hepe 500 mL 500 mL 7) Classical Media
12-117F BE12-117F Med 199 w/EBSS, L-Gln w/25 mM Hepe 500 mL 500 mL 7) Classical Media
12-117Q 12-117Q Med199 w/EBSS,Gln, HEPE2.2g/L NaHCO3 1 L 1 L 7) Classical Media
N/A BE12-117F/12 Med199 w/EBSS,L-Gln,HEPES BOX of 12 BOT 12 × 500 mL 7) Classical Media
12-119F BE12-119F Medium 199 w/ EBSS 500 mL 500 mL 7) Classical Media
12-109F BE12-109F Medium 199 w/ HBSS 500 mL 500 mL 7) Classical Media
12-611Q 12-611Q MEM Eagle EBSS w/ L-Gln 1 L 1 L 7) Classical Media
12-611Q/12 12-611Q/12 MEM Eagle EBSS w/ L-Gln 12 × 1 L 12 × 1L 7) Classical Media
12-125Q 12-125Q MEM Eagle EBSS w/o L-Gln 1 L 1 L 7) Classical Media
12-125Q/12 12-125Q/12 MEM Eagle EBSS w/o L-Gln 12 × 1 L 12 × 1 L 7) Classical Media
12-736F 12-736F MEM Eagle EBSS, Maint., w/FBS+, 500 mL 500 mL 7) Classical Media
12-137F 12-137F MEM Eagle HBSS w/o L-Gln w/HEPES, 500 mL 500 mL 7) Classical Media
04-719Q 04-719Q MEM Eagle Joklik, w/L-Gln w/o Ca++, 1 L 1 L 7) Classical Media
13-114E BE13-114E MEM eagle NEAA (100X) 100 mL 100 mL 7) Classical Media
13-607C 13-607C MEM Eagle Vitamin mix (100x), 50 mL 50 mL 7) Classical Media
12-125F/12 BE12-125F/12 MEM Eagle w/EBSS, w/o L-Gln, 12 × 500 mL 12 × 500 mL 7) Classical Media
12-125F BE12-125F MEM Eagle with EBSS w/o L-Gln, 500 mL 500 mL 7) Classical Media
12-684F BE12-684F MEM Eagle-EBSS (10x) w/o L-Gln, 500 mL 500 mL 7) Classical Media
12-611F/12 BE12-611F/12 MEM Eagle-EBSS 12 × 500 mL 12 × 500 mL 7) Classical Media
12-611F BE12-611F MEM Eagle-EBSS 500 mL 500 mL 7) Classical Media
N/A BE12-662F/12 MEM Eagle-EBSS w/ NEAA BOX of 12 bottles 12 × 500 mL 7) Classical Media
12-662F BE12-662F MEM Eagle-EBSS w/ NEAA w/o L-Gln 500 mL 500 mL 7) Classical Media
06-174G BE06-174G MEM Eagle-EBSS with NEAA w/o CaCl2 450 mL 450 mL 7) Classical Media
12-127F BE12-127F MEM Eagle-HBSS w/o L-Gln, 500 mL 500 mL 7) Classical Media
120
Complete Product List
Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE
N/A BE12-136F/12 MEM-Eagle-EBSS w/HEPES BOX of 12 bottles 12 × 500 mL 7) Classical Media
12-136Q BE12-136Q MEM-Eagle-EBSS w/HEPES w/o L-Glut 1 L 1 L 7) Classical Media
12-136F BE12-136F MEM-Eagle-EBSS w/HEPES w/o L-Glut 500 mL 500 mL 7) Classical Media
12-923E 12-923E NCTC109 w/EBSS,Gln hybridoma, 100 mL 100 mL 7) Classical Media
344018 344018 PC-1 Serum Free Media Component of 77232 500 mL 7) Classical Media
04-648F 04-648F Richter’s CM Medium 500 mL 500 mL 7) Classical Media
12-115Q/12 12-115Q/12 RPMI 1640 w/ 25mM HEPES 12 × 1 L 12 × 1 L 7) Classical Media
12-115F/12 BE12-115F/12 RPMI 1640 w/ 25mM HEPES 12 × 500 mL 12 × 500 mL 7) Classical Media
12-115F BE12-115F RPMI 1640 w/ 25mM HEPES 500 mL 500 mL 7) Classical Media
N/A BE12-115F/U1 RPMI 1640 w/ 25mM HEPES and U1 500 mL 500 mL 7) Classical Media
12-702Q 12-702Q RPMI 1640 w/ L-Gln 1 L 1 L 7) Classical Media
12-702Q/12 12-702Q/12 RPMI 1640 w/ L-Gln 12 × 1 L 12 × 1 L 7) Classical Media
BE12-752F BE12-752F RPMI 1640 w/ L-Glut w/o glucose 500 mL 500 mL 7) Classical Media
12-702F/12 BE12-702F/12 RPMI 1640 w/ L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media
12-702F BE12-702F RPMI 1640 w/ L-Glutamine 500 mL 500 mL 7) Classical Media
N/A BE12-702F/U1 RPMI 1640 w/ U1 500 mL 500 mL 7) Classical Media
N/A BE12-702F/U1/12 RPMI 1640 w/ U1 BOX of 12 bottles 12 × 500 mL 7) Classical Media
N/A BE12-115F/U1/12 RPMI 1640 w/ U1 BOX of 12 bottles 12 × 500 mL 7) Classical Media
12-167Q 12-167Q RPMI 1640 w/o L-Gln 1 L 1 L 7) Classical Media
12-167F/24 12-167F/24 RPMI 1640 w/o L-Gln 24 × 500 mL 24 × 500 mL 7) Classical Media
12-918F BE12-918F RPMI 1640 w/o L-Gln and PR (500 mL) 500 mL 7) Classical Media
12-167Q/12 12-167Q/12 RPMI 1640 w/o L-Glutamine 12 × 1L 12 × 1 L 7) Classical Media
12-167F/12 BE12-167F/12 RPMI 1640 w/o L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media
12-167F BE12-167F RPMI 1640 w/o L-Glutamine 500 mL 500 mL 7) Classical Media
12-115Q 12-115Q RPMI 1640 with HEPES and L-Gln, 1 L 1 L 7) Classical Media
N/A BE04-558F RPMI1640 w/ 25mM HEPES w/o Gln, 500 mL 500 mL 7) Classical Media
09-774E 09-774E RPMI1640,HEPES, Pen, Strep&Glut, 100 mL 100 mL 7) Classical Media
09-774F 09-774F RPMI1640,HEPES, Pen, Strep&Glut, 500 mL 500 mL 7) Classical Media
04-525F N/A STD RPMI1640w/MOPSw/L-Glnw/oNaHCO3,
500 mL
500 mL 7) Classical Media
BE02-019F BE02-019F William’s Med E w/o PR, L-Gln 500 mL 500 mL 7) Classical Media
N/A BE12-761F William’s Medium E w/o L-Gln 500 mL 500 mL 7) Classical Media
02-059E BE02-059E STD FeCitrate100x-PowerCHO™-2KIT,100 mL bt 100 mL Proteins + Reagents
BE02-040E BE02-040E Glucose stock solution 100g/L , 100 mL 100 mL Reagents + ProCHO +
PowerCHO™
121
Complete Product List
Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com
EuropeGermanySartorius Stedim Biotech GmbHAugust-Spindler-Strasse 1137079 Goettingen
Phone +49.551.308.0Fax +49.551.308.3289
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Phone +86.20.37618687 | 37618651Fax +86.20.37619051
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Phone +91.80.4350.5250Fax +91.80.4350.5253
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Phone +60.3.8996.0622Fax +60.3.8996.0755
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Phone +82.31.622.5700Fax +82.31.622.5799
www.sartorius-stedim.comFor more information, you can contact us at [email protected]/media-buffer
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