d e n t a l m a t e r i a l s 3 0 S ( 2 0 1 4 ) e1–e180 e151

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Effects of light-cured MTA like material ondirect pulp capping

F. Petrolo ∗, A. Comba, M. Scansetti, M.Alovisi, D. Pasqualini, E. Berutti, N. Scotti

University of Turin, Department of SurgicalSciences, Turin, Italy

Purpose: To evaluate the effects and the success of Ther-aCal, a new light-cured MTA as a pulp capping materialcomposed of calcium silicate (Portland cement) and resin,compared to reference pulp capping material (self-etchingadhesive system and glass ionomer cement).

Methods and materials: 60 teeth were involved in thisin vivo study, 20 for each pulp capping material. The expo-sure of pulp, that occurred during deep caries excavation, wasrandomly with: (G1) self-etching adhesive system (Protect-Bond, Kuraray), (G2) CVI (Fuji IX, GC) and (G3) TheraCal (Bisco)were applied according to the manufacturer’s instructions.Direct composite restoration was then performed. Patientswere recalled at one week, six months, one and two yearsfollow-up.

Results: After 2 years, in Group 1 teeth were vital at 83.3%,in Group 2 at 66.6% and in Group 3 at 93.3%.

Conclusion: Teeth with pulp exposure may be cappedsuccessfully with a light-cured MTA-like material. Furtherfollow-up controls and an increased number of patients arenecessary to confirm the results.

http://dx.doi.org/10.1016/j.dental.2014.08.308

308

Biocompatibility of EDC cross-linkeddemineralized dentin collagen matrix

G. Turco 1,∗, E. Marsich 1, A. Mazzoni 1, R. DiLenarda 1, M. Cadenaro 1, F.R. Tay 2, D.Pashley 2, L. Breschi 3

1 University of Trieste, Trieste, Italy2 Georgia Regents University, College of DentalMedicine, Augusta, GA, USA3 University of Bologna, Bologna, Italy

Purpose: The dentin organic matrix has a specific nanos-tructural organization characterized by a complex network oftype I collagen fibrils representing approximately 90 wt% ofthe dentin organic phase. Collagen fibrils can be cross-linkedto become more resistant to collagenolysis. In particular, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride(EDC), an imide-based zero-length cross-linking agent, hasbeen shown to be effective for cross-linking dentin collagenand altering the three-dimensional structure of endogenousdentin proteases. The purpose of this study was to investigatein vitro biocompatibility (cell adhesion and proliferation) ofEDC cross-linked collagen matrix.

Methods and materials: Dentin slabs (1.0 ± 0.1 mm thick)were obtained from the mid-coronal portion of noncarioushuman molars using a slow-speed diamond saw (Isomet 5000,

Fig. 1 – Cellular viability as a function of time. MTS assaywas performed at days 0, 1 and 7 after initial seeding. Dataare normalized on the initial signal (day 0).

Buehler Ltd.) under continuous water-cooling. Dentin slabswere completely demineralized in 10 wt% phosphoric acid(pH = 1) at 25 ◦C for 24 h. Demineralized dentin slabs were thor-oughly rinsed in deionized water under constant stirring at4 ◦C for 72 h. Collagen slabs were then cut into circular disks(diameter 4.0 ± 0.2 mm; thickness 1 mm) by means of a surgi-cal biopsy punch. Collagen disks were then treated with 0.5 MEDC solution (pH = 6.3) for 60 s and rinsed with distilled waterfor 10 min. Dry collagen specimens were then reconditionedin complete medium (DMEM) for 24 h. A cell suspension of3000 osteosarcoma cells (MG63) was loaded over the wholeupper surface of collagen disks. After 4 h, the scaffolds wereplaced into fresh, sterile 24-well culture plates and 1 mL ofcomplete medium was added. Viability and growth rate ofMG63 cell were assessed as a function of time using the MTSassay according to the protocol provided by the manufacturer.

Results: The preliminary results of the viability and growthrate of MG63 cells are shown in Fig. 1. Proliferation up to oneweek clearly shows that cells remained viable on the EDCcross-linked collagen matrix. Cell number increased on EDC-treated disks more than on control disks.

Conclusion: Collagen treatment using an EDC solution didnot alter the cellular proliferation rate. Further studies areneeded to prove the biocompatibility of EDC treated collagenwith culture times longer than one week.

Keywords: Collagen; Cross-linkers; EDC

http://dx.doi.org/10.1016/j.dental.2014.08.309