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BioAnalyzer; Agilent 2100 (Detect Instrument Picture to Jump to that section of SOP)

Prepared by: Bob Morrison

STLCC CPLC, Instrumentation Specialist

Initial Feb 2008, Latest Rev June 2013

IKA MS2 Minishaker

Vortexer for use with

Agilent 2100Bioanlyzer

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Agilent BioAnalyzer: Connections and Startup

The Agilent 2100 Bioanalyzer is very useful tool in analyzing RNA

quality for real-time PCR and microarray experiments. It combines

a miniature of gel electrophoresis and scanning spectrophotometry

to allow analysis of quality of RNA, DNA and protein samples using

very small amounts of material, i.e., nanograms or even picograms

of total RNA

2100 Bioanalyzer Specifications

Weight 10 kg (22 lbs)

Dimensions (w × d × h) 162 × 412 × 290 mm (6.4 × 16.2 × 11.4 in)

Line voltage 100-240 VAC

Line frequency 50-60 Hz

Power consumption 60 VA

Ambient operating temperature5-40 °C (41-104 °F)

Software: V B.02.08.SI48 SR1

1. Connect device(s) to host CPU via

RS232 directly or via RS232/USB

adapter

2. On/Off toggle (rear panel, extreme lower

center by power plug)

Electrode Cartridge Type Stamped:

(1) Electrophoresis

(2) Flow cytometry

3. Wait for light to reach steady green

Light: Green (ready), Blinking Green

(busy), Orange (diagnostics running),

Red (not ready)

4. Logon: Adminstrator/3000hanover

5. Start Expert 2100 Software after

green lights on all instruments

Serial Number of Instrument

Bioanalyzer: Agilent 2100, Bayonet Cartridge

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Link to Agilent Bayonet Removal

guide (pdf)

Users.stlcc.edu/Departments/fvbio/Bioanalyzer_Agilent_Changing_16-Pin_Bayonet_Cartridge.pdf

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BioAnalyzer: Agilent 2100 Expert Software Startup Screen

1. Wait for Expert2100 software to

detect each attached instrument

located at respective Com ports.

(will be Com1 if RS232 direct, or

Com >4 if RS232/USB is used)

2. Note Serial Numbers of instruments

detected and checklist on each.

DE 72901434

DE 54700853

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BioAnalyzer : Agilent 2100 Manuals, Reference Materials

Link to Agilent Protein 80 Kit Quick Start Guide (pdf)

Link to Agilent 2100 Expert Users Guide (pdf)

Link to U of Virginia Lab: Scanning Gels using GS-800 (pdf)

Link to Agilent Protein 230 Kit Quick Start Guide (pdf)

Link to Agilent Protein 80 Kit Ebook (pdf)

Link to Agilent 2100 Troubleshooting Guide (pdf)

Link to Agilent RNA 6000 Nano Kit (w BB notes) Quick Start Guide (pdf)

http://www.chem.agilent.com/Library/usermanuals/Public/G2938-90063_Protein80Assay.pdfUsers.stlcc.edu/Departments/fvbio/BioAnalyzer_Agilent2100_Expert_User_Manual.pdfhttp://www.itc.virginia.edu/achs/documents/ACHS-103.pdfUsers.stlcc.edu/Departments/fvbio/Bioanalyzer_Agilent2100_Protein230Kit_Analysis.pdfUsers.stlcc.edu/fvbio/Bioanalyzer_Protein80_ebook.pdfUsers.stlcc.edu/Departments/fvbio/BioAnalyzer_Agilent2100_Troubleshooting_Guide.pdfUsers.stlcc.edu/Departments/fvbio/BioAnalyzer_Agilent_RNA6000_Nanokit.pdf

BioAnalyzer: Agilent, Recent (June 2011) Error Message

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With the dual Bioanalyzers running on

USB/RS232 adapters, this error message

has been encountered. See the next slide

for a method to correct the problem.

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BioAnalyzer: Agilent, Error Message Potential Fix

Some errors have been corrected by

selecting this button and typing in the

Device ID name in the pop-up window

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BioAnalyzer: Agilent2100, Insert Sample Chip

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BioAnalyzer: Agilent Expert-Assay-Electrophoresis-Protein Options

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BioAnalyzer: Agilent-Expert2100-Proteinchip-Run Checklist completed

Select Start Run after the

Checklist is complete

Select Start Run after the

Checklist is complete

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BioAnalyzer: Agilent2100- Observations during the Chip Run

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BioAnalyzer: Agilent2100, Data context, Chip Summary

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BioAnalyzer: Agilent2100 During the Run -Measurement

Page 13

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BioAnalyzer: Agilent2100, Electropherogram tab

Page 14

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BioAnalyzer: AgilentExpert 2100,Example-Protein 80 Assay Results

Page 15

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BioAnalyzer: Agilent2100, Options after the Run

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BioAnalyzer: Agilent Expert-INSTRUCTOR Instrument-Diagnostics-Selectall-Start

Diagnostics Test requires two special

chips: Electrode Chip and Optics

Chip. Locate these before beginning

diagnostics. Also have a

blank/empty chip handy.

Follow software instructions during

test.

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BioAnalyzer: Agilent2100 INSTRUCTOR

Expert-Instrument-Diagnostics-Follow Instructions

Diagnostics Test requires two special

chips: Electrode Chip and Optics

Chip. Locate these before beginning

diagnostics. Also have a

blank/empty chip handy.

Follow software instructions during

test.

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BioAnalyzer: Agilent2100 Expert INSTRUCTOR

-Instrument-Diagnostics- Report

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BioAnalyzer: Agilent2100 Expert-Instrument-Diagnostics-LogBook-Print-pdf

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BioAnalyzer: Agilent2100 Expert-Assay-Electrophoresis-DNA Options

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BioAnalyzer: Agilent2100 Expert-Assay-Flow Cytometry

BioAnalyzer: Vortexer; IKA Works-MS2 Minishaker,

(use with Agilent 2100 Bioanalyzer)

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Universal, small-sized shaker. With an orbit

of 4.5 mm and a speed range of 0 - 2,500

rpm, the MS 2 is suitable for all small

vessels. - Electronic speed control -

On/Off

Set rpm using

analog dial

Fitting (not shown)

for Agilent 2100

Bioanalyzer Plates

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BioAnalyzer : Other Notes and Options

• Future home of other detailed procedures or printout options ….