Bio 525/ Spring, 2010

Nuclear Architecture and Genomic Function

Session 6, Part II: The Nucleolus: rRNA Gene Organization and Function: Background and Figures for Koberna et al.,2002; Kalmárová et al., 2007

Nucleolus

• The Nucleolus (‘tiny nucleus”) is a reticular fibrogranular structure in the nucleus that is specialized for transcription of ribosomal RNA and its packaging into pre-ribosomal subunits.

• The genes for ribosomal RNA are highly amplified and located on five different chromosomes called the nucleolar organizer regions (NOR).

• rDNA genes are concentrated within the numerous fibrillar centers (fc) that compose the nucleolus.

• rRNA is believed to be transcribed at the borders of fc and the dense fibrillar component (dfc) where ribosomal proteins associate to form pre-ribsosomal RNP particles but this is still controversial.

• Progressive processing of the pre–rRNP particles occurs within the granular component (gc) where mature ribosomal subunits are released for transport into the cytoplasm.

• The massive level of rRNA transcription is illustrated by the “Christmas tree” structures composed of repeating nascent rRNP strands that grow in assembly line fashion along the rDNA transcription units.

Raska, TRENDS in Cell Biology 2003, Figure 1. Schematic representation of human ribosomal RNA (rRNA) gene-array, rRNA gene and rRNA gene transcript.

Schematic representation of ribosome

Nissen et al., Science : Vol. 289, pp. 920 - 930

A Beautiful Complexity of the Ribosomal Structure

The Nobel Prize in Chemistry in 2009 was

awarded to Venkatraman Ramakrishnan,

Thomas A. Steitz and Ada E. Yonath for

having showed what the ribosome looks

like and how it functions at the atomic

level.

Mammalian Cell as Seen by Light Microscope

(courtesy to lamondlab.com)

Nuclear Transcription in Mammalian Cells

The RNA transcription signal is present in numerous sitesthroughout nucleus. The strongest signal is accumulated within nucleoli.

Nucleoli are sites of expression of specific genes: ribosomal genes (rDNA)

The ribosomal RNA genes are highly amplified (total of ~400 copies in human cells) at the p-arms (Nucleolar Organizer Regions) of these 5 human NOR-chromosomes

Courtesy to http://www.dundee.ac.uk

Visualization of Ribosomal Genes on Metaphase Chromosomes

Controversy behind the localization of nucleolar transcription sites

Transcription sites are located exclusively in FCs

Thiry et al; RNA. 2000, 12:1750-61. Thiry et al; Trends Cell Biol. 2001,

4:147

Cheutin et al; J Cell Sci. 2002, 115: 3297-307.

Mais et al; J Struct Biol. 2002, 140: 214-26.

Derenzini et al; J Histochem Cytochem. 2006, 54:131-45.

Transcription sites are located exclusively in DFCs

Melcak I et al; J Struct Biol. 1996, 116: 253-63.

Cmarko et al; Histochem Cell Biol. 2000, 113:181-7.

Gonzalez-Melendi et al; Plant J. 2001, 3: 223-33.

Stanek D et al; Chromosoma. 2001, 110: 460-70.

Koberna et al; J Cell Biol. 2002 157: 743-8.

Koberna et al., J Cell Biol. 2002;157(5):743-8.

Ribosomal Genes in Focus: New Transcripts Label the Dense Fibrillar

Components and Form Clusters Indicative of "Christmas Trees" in situ.

Major Conclusions of Koberna et al., 2002

1) At the fluorescence microscopic level, ribosomal genes are organized in discrete foci. # of foci varied from 10 to 40 in different cells (Fig 1 & 2).

2) rDNA transcription sites are strictly limited to nucleolar DFC and DFC/FC transition zones. This suggests a strict structure- function compartmentalization of the nucleolus (Fig 3).

3) (Correlation of fluorescence and electron microscopy)

Each fluorescent nucleolar transcription site (focus) at the ultrastructural level corresponds to group of rDNA genes located within DFC that surround individual FC. Since in HeLa cells ~ 150 of ribosomal genes are transcriptionally active (Jackson et al., 1993; EMBO J; 12: 1059-1065) each focus represents a group in average of ~4 to15 active genes (Fig 1- 4).

Conclusion 1 (Fig 1 & 2)

In HeLa cells ribosomal genes are organized in

discrete groups or foci. Number of foci varied

from 10 to 40 in different cells.

Koberna et al JCB 2002, Figure 1.

Localization of ribosomal genes in HeLa cells.

Fluorescence mapping of ribosomal genes (A and C, yellow), phase contrast image (B and C, blue), and the merge image (C) are shown. Ribosomal genes are located in a number of fluorescent foci. Most of the foci are situated in the nucleolus. Two foci are situated in the perinucleolar chromatin (arrowheads). (D) EM mapping of ribosomal genes. The signal (arrowheads) is located in DFC. (f, d and g) FCs, DFCs, and granular components, respectively

Bars: (A–C) 10 ; (D) 200 nm.

(C–E) Light microscopic image of ribosomal genes (C and E, red) and transcription sites (D and E, green). The merged image is E. Most of rDNA foci exhibit the transcription activity. An rDNA focus without transcription signal is indicated by the arrow. Bar: 5 m.

Koberna et al JCB 2002, Figure 2. LM mapping of nucleolar transcription in HeLa cells. High magnification is used to see details of nucleolar structure

Ribosomal Genes Transcription Merged

Conclusion 2

rDNA transcription sites are strictly limited to

nucleolar DFC and DFC/FC transition zones. This

suggests a strict structure functional

compartmentalization of nucleolus (Fig 3).

Koberna et al JCB 2002, Figure 3.

EM mapping of nucleolar transcription in HeLa cells. (A) Nucleolar EM transcription signal observed in hypotonically treated cells is confined to the DFC. (B–D) EM localization of transcription in thin-sectioned permeabilized cell. (C and D) Detailed parts of B. Nucleolar transcription signal consists of numerous clusters of gold particles accumulated in the DFC.

Conclusion 3 (Correlation of fluorescence and electron

microscopy)

Each fluorescent nucleolar transcription site on

ultrastructural level corresponds to group of rDNA

genes located within DFC that surround individual

FC. Since in HeLa cells ~ 150 of ribosomal genes

are transcriptionally active (Jackson et al., 1993;

EMBO J; 12: 1059-1065) each focus represents a

group in average of ~4 to 15 active genes (Fig 1-4).

Immunolabeling tools for Light and Electron Microscopy

Raska, TRENDS in Cell Biology 2003, Box 1

Concomitant LM and EM Mapping of Transcription Signal in a Thin Section of a Permeabilized Cell.

(B) Detailed part of A. The colored insert in A represents the wide-field LM picture of the same area depicting newly synthesized RNA (red) and DNA counterstained with YOYO (green). (C) Merge of an inverse EM image from A in cyan, and a transcription LM signal from the upper insert in red. Note the overlap of the LM and EM transcription signals (white areas). Bars: (A) 0.5 ; (B) 0.2 .

Koberna et al JCB 2002, Figure 4.

Proposed model of the arrangement of Christmas tree structures inanimal cells. This model suggests that FC represent sites of storage/ reassembly of the rDNA transcription machinery as well as inactive rDNA. DFC represent sites of rRNA synthesis/processing, while in GC the final steps take place for rRNA processing and assembly of pre-ribosomal particles.

Raska, TRENDS in Cell Biology 2003, Figure 5

Major Conclusions of Koberna et al., 2002

1) In HeLa cells ribosomal genes are organized in discrete groups or foci.

Number of foci varied from 10 to 40 in different cells (Fig 1 & 2).

2) rDNA transcription sites are strictly limited to nucleolar DFC and DFC/FC

transition zones. This suggests a strict structure functional

compartmentalization of nucleolus (Fig 3).

3) (Correlation of fluorescence and electron microscopy)

Each fluorescent nucleolar transcription site on ultrastructural level

corresponds to group of rDNA genes located within DFC that surround

individual FC. Since in HeLa cells ~ 150 of ribosomal genes are

transcriptionally active (Jackson et al., 1993; EMBO J; 12: 1059-1065) each

focus represents a group in average of ~4 to15 active genes (Fig1- 4).

Spatio-temporal dynamics at rDNA foci: Global switching between DNA replication

and transcription

Pliss et al., J Cell Bichem. 2005; 94: 554-565

Major Conclusion of Pliss et al., 2005

The rDNA foci are the functional units for coordinating replication and transcription of the rRNA genes in space and time. This involves a global switching mechanism, active from mid to late S-phase, for turning off transcription and turning on replication at individual rDNA foci. Once all the rRNA genes at individual foci are replicated, these higher order chromatin domains are reprogrammed for transcription.

Figure 2. Electron microscopic localization of replication, transcription, and fibrillarin in HeLa cells.

A: Electron microscopic localization of transcription in thin sectioned cells. B: Fluorescence microscopy image of nucleolar transcription pattern seen in cells synchronized in S-phase. C: Enlarged outlined area in (A). D: The nucleolar DNA replication: the signal is found mainly in the DFC (arrows). Some DNA replication occurs also in the perinucleolar chromatin (arrowheads). E: Fibrillarin –a marker of the rRNA processing is accumulated in the DFC.

Figure 1. Imaging of rDNA, nucleolar transcription, replication, and fibrillarin in HeLa cells. A-D: Mapping of rDNAs (A, green), transcription (B, red), and fibrillarin (C, blue); (D) merged image. Most rDNA foci co-localize with transcription signal and fibrillarin. Two rDNA foci (arrows) do not overlap with transcription and fibrillarin signals. E-G: Signals of replication (E, green) and rDNAs (F, red). One rDNA focus (arrow) overlaps with replication signal at the merged image (G). H-K: Mapping of replication (H, green), rDNAs (I, red), and transcription (J, blue) in the nucleolus. The rDNA focus, which co-localizes with replication signal (arrow), does not overlap with transcription in the merged image (K). Foci inactive in both DNA replication and transcription are occasionally observed (see arrowhead in K). L-O: Mapping of replication (L, green), rDNAs (M, red), and fibrillarin (N, blue) in the nucleolus. In the merged image (O), no fibrillarin signal is seen in the replicating rDNA foci (arrows), while magenta colored sites (arrowheads) co-localize with fibrillarin and do not replicate DNA .

Figure 3. Schematical representation of rDNA foci dynamically switched on and off for DNA replication or transcription.

In this model, individual rDNA foci are depicted as being along the borders of the nucleolar FC/DFC regions. Each focus is a functional unit containing multiple rDNA genes switched on for either DNA replication (green) or transcription (red). Following the completion of DNA replication at individual foci, transcriptional activity is switched back on.

Major Conclusion of Pliss et al., 2005

The rDNA foci are the functional units for coordinating replication and transcription of the rRNA genes in space and time. This involves a global switching mechanism, active from mid to late S-phase, for turning off transcription and turning on replication at individual rDNA foci. Once all the rRNA genes at individual foci are replicated, these higher order chromatin domains are reprogrammed for transcription.

Kalmárová et. al., J Struct Biol. 2007;160(1):49-56.

Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli.

Representation of chromosome territories in the fibroblast nuclei

Bolzer et al., PLoS Biol. 2005

Major Conclusions of Kalmárová et al., 2007

1)Majority of the NOR bearing chromosome territories are spatially associated with the nucleolus (Fig 1 and Fig 3).

2) NORs are usually located within the nucleolus regardless of their transcription activity (Fig 4)

3) NOR bearing chromosome territories which are not associated with the nucleolus may loop out rDNA sequences into the nucleolus (Fig.2 and Fig 5)

Conclusion 1 (Fig 1 and 3)

Majority of the NOR bearing chromosome territories are spatially associated with

the nucleolus

Kalmárová et. al., Fig. 1. Association of the chromosomes 13 and 15 with nucleoli in interphase HeLa (a) and LEP (b) cells. The transformed HeLa cells contain three homologs of the chromosomes 13 and four homologs of the chromosome 15. The primary LEP cells have normal karyotype with two chromosomes 13 and 15. In both HeLa and LEP cells, the chromosome homologs 15 appeared usually in close association with nucleoli, while one of the homologs 13 was often distanced from the nucleoli (arrow).

Fig. 3. Transcription competence of the NORs belonging to different chromosomes correlates with nucleolar associations of these chromosomes. The percentage of the nucleoli closely associated chromosomes was counted in HeLa and LEP cells.

Conclusion 2 (Fig 4)

NORs are usually located within the nucleolus regardless of their transcription activity

Fig. 4. Most HeLa and LEP cells contain no extranucleolar rDNA. (a) rDNA (red), fibrillarin (green) and merged image in a HeLa cell. No rDNA signals are present outside the fibrillarin-positive nucleoli. The arrows indicate Cajal bodies. Bar: 10 lm. (b) The percentage of extranucleolar rDNA foci in HeLa and LEP cells. The extranucleolar rDNA foci are absent in about 73% of HeLa cells and in about 87% of LEP cells.

Conclusion 3 (Fig.2 and Fig. 5)

NOR bearing chromosome territories which are not associated with the nucleolus may loop out rDNA sequences into the nucleolus

Kalmárová et. al., Fig. 2. A thin protrusion between the extranucleolar chromosome territory and nucleolus. Two chromosome homologs 14 (in red; arrows) in the HeLa cell are closely associated with fibrillarin-positive nucleoli (in green). The third chromosome homolog 14 (in red) is distanced fromnucleolus and connected to it via a long chromatin protrusion that endswith a thickening at the contact point with the nucleolus.

Fig. 5. Triple labeling of chromosomes 14, fibrillarin and rDNA in aHeLa cell. The chromosomes are shown in blue, fibrillarin in green, and rDNA in red. Two chromosome territories are closely associated with nucleoli (arrowheads). The third chromosome territory (arrow) is distanced from the nucleolus and is not colocalized with rDNA signal. Since no rDNA signals were found outside the nucleoli, the NORbelonging to this chromosome should be in the nucleolus. Bar: 10 lm.

Major Conclusions of Kalmárová et al., 2007

1)Majority of the NOR bearing chromosome territories are spatially associated with the nucleolus (Fig 1 and Fig 3).

2) NORs are usually located within the nucleolus regardless of their transcription activity (Fig 4)

3) NOR bearing chromosome territories which are not associated with the nucleolus may loop out rDNA sequences into the nucleolus (Fig.2 and Fig 5)