BIO 185THE BIOLOGY OF THE CELL

Winter 2014  Instructors: Walter J. Bruyninckx  Office: Science Center,217  Phone - office: 866-7242 - home: 866-4522 (before 9:00 PM) e-mail: bruyninx@hanover.edu  Office Hours: Monday 9:00 - 10:00 am

Tuesday 12:00 - 5:00 pmWednesday 12:00 - 4:00 pm

Thursday 12:00 - 3:00 amFriday 9:00 – 10:00 am

Venus in front of the mirror (1613)

Bacchus (1638)

The Union of Earth and Water (1618)

Bio 185

Bio 185. Cell and Molecular Biology

Molecules of the living materialCellular organization of living materialEnergy metabolismMolecular biology: central dogmaCancer biology

Bio 185Cell and Molecular Biology

ACTIVITIES:

1.Lectures2.Preparing a Notebook3.Study 4.Readings5.Lab6.Exams.

Due AuthorsScientificAmerica

Title Pages

1/11Gerstein Mark and

Levitt MichaelNovember

1998Simulating water and molecules of life 100-105

1/18 Welch William J. May 1993 How cells respond to stress 56-64

2/01Goldberg Alfred L., Elledge

Stephen J. and Harper J. Wade

January 2001

The cellular chamber of doom 68-73

2/15Rothman James E. and Orci

LelioMarch 1996

Budding vesicles in living cells 70-75

2/22 McCarthy Richard E 1985H+ ATPases in oxidative and

photosynthetic phosphorylation

BioScience 35 p7-

30

3/15Lau Nelson C. and Bartel

David P.August 2003

Censors of the genome 34-41

3/22 Ast Gil April 2005 The alternative genome 58-65

4/05Scott John D. and Pawson

TonyJune 2000 Cell communication: the inside story 72-79

4/12 Weinberg Robert A.September

1996How cancer arises 62-70

Exam date Lectures points %

Exam 1 01/25 The molecular basis of Life (Lecture1-11) 80 13.33Exam 2 02/08 Cell structure (Lecture 12-20) 80 13.33Exam 3 03/08 Energy metabolism (Lecture 21-28) 80 13.33Exam 4 03/28 Molecular Biology (Lecture 29-40) 80 13.33Exam 5 04/15 Cancer (Lecture 40-49) 80 13.33

Cmprh. ex 04/15 Lectures (1-49) 50 8.33Lab exam 04/09 50 8.33

Lab reports 50 8.33Readings 50 8.33

Total 600

Bio 185. Molecular Cell BiologyGrading

Bio 185Cell and Molecular Biology

http://vault.hanover.edu/~bruyninx/

Bio185Bruyninckx

Bio185ContentBio185ExercisesBio185LabDataBio185PowerPointBio185ReadingAssignmentsBio185Syllabus

Bio 185. Molecular Cell Biology

Course policies

3. Course policies. 0. Do NOT wear a baseball cap (in whatever direction) in class or lab.1. Your active and attentive presence in class and lab is expected.2. Any work handed in after the time is due will not be graded.3. All written material turned in for credit must be typewritten and paper-copy (I am sorry, we cannot be your printer). Multiple pages need to be stapled together!4. All writing that is not your own independent work must be properly referenced. Please refer to the Student Handbook (pages 2 to 3) and the policy statement on academic dishonesty.4. There is no opportunity to make up quizzes, laboratories, assignments.5. For safety reasons, eating, drinking and smoking are not permitted in the laboratory or class. Shoes must be worn at all times in the laboratory, as there may be broken glass and other hazardous material on the floor.6. Turn off your cell phone or other similar devices when in class, lecture, or study session.7.Limit use of a computer in class or lab to the scheduled class activities.8.Please, no smelly food!

Wk Date Title Topic

1 01/08 Introduction Topic-12 01/15 Laboratory tools Topic-13 01/22 Introduction to bio-molecules Topic-24 01/29 Characterization of enzymes-1 Topic-35 02/05 Characterization of enzymes-2 Topic-36 02/12 Cell membrane and osmosis Topic 47 02/19 Cell Structure Topic 4

8 03/05 Respiration and fermentation Topic 59 03/12 Photosynthesis Topic 610 03/19 Analysis of proteins Topic 911 03/26 DNA and Cell cycle Topic 7, 812 04/02 Gene regulation: lac operon Topic 1013 04/09 Lab Exam

LAB REPORT FORMAT: (1 report per group!)

LAB TITLE: Properties and Function of Enzymes.Experiment 1.

Goal: one sentence.Method: Relevant.Results: (based on class data). A (mean and SEM) is significantly different

(P<0.05) from B (mean and SEM).Conclusion: A is different from B.Explanation.

Standard Curve Yellow Dye

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1 1.5 2 2.5 3 3.5 4

Concentration (drops)

Op

tica

l Den

sity

at

420

nm

Figure 1. Standard curve of yellow dye at 420 nm. We measured the optical density at 420 nm of four concentrations of yellow dye (1, 2, 3, and 4 drops per sample) using a Spectronix 21 spectrophotometer. The OD of Standard 1 ((0.217 ± 0.0070) was significantly smaller (P<0.05, Student’s t-test) than that of Standard 2 (0.410 ± 0.0057), the OD of Standard 2 was significantly smaller (P<0.05) than that of Standard 3 (0.619 ± 0.0045) and the OD of Standard 3 was significantly smaller (P<0.05) than that of Standard 4 (0.806 ± 0.0087). We calculated the standard curve (linear regression y = 0.1977x + 0.0186, R2 = 0.9996).We measured the optical density at 420 nm of an unknown concentration of yellow dye (OD equals 0.699 ± 0.0054) and used the standard curve to determine its concentration (3.44 drops per sample).