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M.Kato & T.Aoki-1 `bekUEDr&>6'9 ekuUaJy RgknHK AiChzI[ ?!/%? ïʌ ɪȷŁ ʃ2ǨļŁķŃķŃˠ¯˜ƪóìƐȿŃȸɅȿNz]NQaœƣ 112-8610 ƪ®õķİ 2-1-1 ˬƻ Çĺ ưƽķŃȗȎʴǿȿŃˍ 252-0880 ȽĻşȱʌǫŦ§«˔ 1866 ƽȏ˨3ưƽǎȎŃ¹Ɉ 75 Ěķ¹2_`OĶǕ0ȎʶIȗ<àí -©Ǜµʭ2 ǚɲ.çŽ-2ÞōIɢʦ1,ĂD;.>&?2-2_`O3Ƹʿ2ǎȎȿŃ ȸɅ2ƔǮȓȐ°ź2Ś˛1*,ȨʒʠʰEɢʦȪƧɰȪ0_`O.,ư ƽǎȎŃ¹Ŭĭľĕ¹1B),¸ȞF;& *,ǎȎ2©Ǜµʭ3Ƃ˱2Ǜµʭ.3ȣ0DǎȎ2ȗdz13ȯƝƂʗ-30Ȏʶ 2µʭ-E.ʤʮF,;&ʿū2ȸɅƖʏ2˅Ǡ.Ȱ;),©ǛµʭȸɅ 3˷ʺȪ0Ś˛Iˆ©ǛµʭȘȎ2ɄǑȪ0ŸëǰȮIǵ5,;ȗ¿˞Ž2ƔǤ. ,ƲʍɊ2ʥěȎʶ.,_XoºˇȎʶ.,Ɋ;1˚ǡą2¡-ǎȎ Ïƚ*B10)&ȗłƑȡ1©Ǜµʭ3ķ0ʳȑI,;Ķŷ.ʟʆB¼ ĉ©ǛµʭȘȎ2ǔ˂2ĶǕƆ3ʕ˩1˅ó&ȗĉƐɓ2ˑɗ1ơCF$FȗȎĶ ǕƆ7.*0),;ƽ_`O-3&©ǛµʭȘȎI*D'þòí1 ȉȇI,;;0ʜůC©ǛµʭȸɅ1L|h,EʁƔ¡ĬȸɅɯIɑˉ ʬȂI˵;& ƽȏ˨3ʬȂɯ2ƸƬ2ȸɅƐDŽIȭDʾJ'ÞōI;.>;&íȪ0©Ǜµʭ n7ȫǕIʥ;ƽȏ˨I÷á1ǒ<&'ȫǕ2°ź2ȸɅ2ɒ.,' F4ŀƅ; ǎȎȿŃƸéɣ 2:1 2011 BSJ-Review 2:1 (2011)

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Page 1: `bekUEDr&>6' 9 ekuUaJy Rg k n HK AiC hz I[1 ø | } |2m [VU O } q2S ksNn ¹ Õ kN J Õ-16 nmol g-1 FW 9 µg g-1 FW1 0 t . - F, D Nakayama et al. 2006 F. æ ¼ , !F? A o - )& & ), FC2

M.Kato & T.Aoki-1

`bekUEDr&>6'�9� ekuUaJy�

�Rg�k��n�HK� �� AiC��hz I[�

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ïʌ� ɪȷŁ

�ʃ2ǨļŁķŃķŃˠ¯˜ƪóìƐȿŃȸɅȿ�Nz]NQ�aœƣ

112-8610� ƪ®õķİ 2-1-1

ˬƻ� Çĺ

ưƽķŃȗȎʴǿȿŃˍ

252-0880� ȽĻşȱʌǫŦ§«˔ 1866

� ƽȏ˨3ưƽǎȎŃ¹Ɉ 75 Ěķ¹2_��`O��ĶǕ0ȎʶIȗ<à í� -©Ǜµʭ2

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F4ŀ��ƅ�;

ǎȎȿŃƸéɣ 2:1 2011

BSJ-Review 2:1 (2011)

Page 2: `bekUEDr&>6' 9 ekuUaJy Rg k n HK AiC hz I[1 ø | } |2m [VU O } q2S ksNn ¹ Õ kN J Õ-16 nmol g-1 FW 9 µg g-1 FW1 0 t . - F, D Nakayama et al. 2006 F. æ ¼ , !F? A o - )& & ), FC2

N. Tanikawa-1

�}+2"Wek"> *:�}|s|hf�

ʱş ĻDz ʽǐ�˸ĔȘǐƖʏɢĉȸɅǚǔ ɽ�ȸɅƓ 305-8519� ʂĨȱ*�4Ŧʌƽ 2-1

The mechanism of yellow flower coloration of Camellia chrysantha

Key words: aluminum; Camellia chrysantha; flavonols; yellow flower.

Natsu Tanikawa National Institute of Floricultural Science, National Agriculture and Food Research Organization

2-1 Fujimoto, Tsukuba, Ibaraki 305-8519, Japan

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ǎȎȿŃƸéɣ 2:2 2011

BSJ-Review 2:2 (2011)

Page 3: `bekUEDr&>6' 9 ekuUaJy Rg k n HK AiC hz I[1 ø | } |2m [VU O } q2S ksNn ¹ Õ kN J Õ-16 nmol g-1 FW 9 µg g-1 FW1 0 t . - F, D Nakayama et al. 2006 F. æ ¼ , !F? A o - )& & ), FC2

N. Tanikawa-2

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ǎȎȿŃƸéɣ 2:3 2011

BSJ-Review 2:3 (2011)

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BSJ-Review 2:4 (2011)

Page 5: `bekUEDr&>6' 9 ekuUaJy Rg k n HK AiC hz I[1 ø | } |2m [VU O } q2S ksNn ¹ Õ kN J Õ-16 nmol g-1 FW 9 µg g-1 FW1 0 t . - F, D Nakayama et al. 2006 F. æ ¼ , !F? A o - )& & ), FC2

N. Tanikawa-4

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ǎȎȿŃƸéɣ 2:5 2011

BSJ-Review 2:5 (2011)

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ǎȎȿŃƸéɣ 2:6 2011

BSJ-Review 2:6 (2011)

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N. Tanikawa-6

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Development of efficient functional analysis of plant terpene biosynthetic genes:

mainly of sesquiterpene biosynthetic genes Key words: cytochrome P450; pathway engineering; sesquiterpene biosynthesis.

Hisashi Harada

Technology Development Dept., Biotechnology & Drug Discovery Div., KNC Laboratories Co., Ltd.

KNC Bioresearch Center, 1-1-1 Murodani, Nishi-ku, Kobe, 651-2241, Japan

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Diversity of enzymes in isoflavonoid pathway

Key words: isoflavonoid; P450; phytoalexin; prenyltransferase; soybean.

Tomoyoshi Akashi Department of Applied Biological Sciences, Nihon University

Fujisawa, Kanagawa 252–0880, Japan

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BSJ-Review 2:19 (2011)

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BSJ-Review 2:20 (2011)

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ƹǩȖĸƣêɁ2:21˩ 2011 ˪

BSJ-Review 2:21 (2011)

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T. Akashi - 5

�f~�aIƺȱ�˫50˨±�2Ć�ůIȑ 3*2Ëɲʴã˩PT1, PT2, PT3˪IʰŽ�&�2�( PT23gNbǻǽɣ�ŴƳ�&ȳɒ2 EST�N{���-Ş�Ǿǰ�,�D˫ɻ njǾǰȮIǵ�&DŽɓɼƯ1BD glyceollin I 2dząŵ1˅HE(-)-glycinol 4-dimethylallyltransferase˩ G4DT˪-�E�.�H�)&(Akashi et al. 2009)$2ŦgNb2Zs�ʴã�ƚC�1�F&�.�Cl�f~�aIÞŘƺȱ�˫Ɠ&1 3*2���X(PT4, PT5, PT6)Iɷ�'�&$F%FIʵnj-Ǿǰ�",DŽɓɼƯIɭ�.˫PT6 3 glyceollin II, III 2dząŵ1˅HE(-)-glycinol 2-dimethylallyltransferase˩ G2DT˪-�E�.�H�)&;& PT4, PT53$F%FNez��sNn2W�ak���ú5Nez���I|�p�2LWc|f�1 E�.�H�)& � ��Ȗ2W��(Sophora flavescens)�C3z�us�2 8 º1|�p�ĠIʚȘ EʵȰʱ¶ĵ(SfN8DT-1, SfN8DT-2)�Ćļ�F,�E(Sasaki et al. 2008)ȮȻǁ�-3 PT2, PT3, PT4, PT5, PT63 SfN8DT.3æ2W��n1ʼn�,�EgNb-3ʨô2ʬș-Nez��sNnIĠʓ1 E|�p�ĠʚȘʵȰ�dz�˫īƾ0DŽɓIƂ*B�10)&.Ųļ�FELWc|f

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Akashi, T., Sawada, Y., Shimada, N., Sakurai, N., Aoki, T. & Ayabe, S. 2003. cDNA cloning and biochemical characterization of S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway. Plant Cell Physiol. 44: 103-112.

Akashi, T., Aoki, T. & Ayabe, S. 2005a. Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis. Plant Physiol. 137: 882-891.

ė 6� ƹǩ2|�p�ĠʚȘʵȰ2âĵȮȻǁȮȻǁ3ClastalWIǵ�ʟˎȸąǔ-¾ŵ�&

ƹǩȖĸƣêɁ2:22˩ 2011 ˪

BSJ-Review 2:22 (2011)

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T. Akashi - 6

Akashi, T., Ishizaki, M., Aoki, T. & Ayabe, S. 2005b. Isoflavonoid production by adventitious root cultures of Irs germanica (Iridaceae). Plant Biotechnol. 22: 207-215.

Akashi, T., Sasaki, K., Aoki, T., Ayabe, S. & Yazaki, K. 2009. Molecular cloning and characterization of a cDNA for pterocarpan 4-dimethylallyltransferase catalyzing the key prenylation step in the biosynthesis of glyceollin, a soybean phytoalexin, Plant Physiol. 149: 683-693.

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Sawada, Y. & Ayabe, S. 2005. Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue. Biochem. Biophys. Res. Commun. 330: 907–913.

Shimada, N., Sato, S., Akashi, T., Nakamura, Y., Tabata, S., Ayabe S. & Aoki, T. 2007. Genome-wide analyses of the structural gene families involved in the legume-specific 5-deoxyisoflavonoid biosynthesis of Lotus japonicus. DNA Res. 14: 25-36.

ƹǩȖĸƣêɁ2:23˩ 2011 ˪

BSJ-Review 2:23 (2011)

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Anthocyanin modification catalyzed by vacuolar proteins.

Key words: anthocyanin; carnation; delphinium; glucosyltransferase.

Nobuhiro Sasaki Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of

Agriculture and Technology 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan

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BSJ-Review 2:24 (2011)

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BSJ-Review 2:25 (2011)

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BSJ-Review 2:26 (2011)

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BSJ-Review 2:27 (2011)

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k�p{ Abe, Y., Tera, M., Sasaki, N., Okamura, M., Umemoto, N., Momose, M., Kawahara, N., Kamakura, H.,

Goda, Y., Nagasawa, K. & Ozeki, Y. 2008. Detection of 1-O-malylglucose: pelargonidin

Figure 2� l�zMpO�˒ɠś1��,Ɔļ�FEL�m_Lp�ɟȰąŵȷʗė

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BSJ-Review 2:28 (2011)

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N. Sasaki-6

3-O-glucose-6''-O-malyltransferase activity in carnation (Dianthus caryophyllus). Biochem. Biophys. Res.

Commun. 373: 473-477.

D'Auria, J.C. 2006. Acyltransferases in plants: a good time to be BAHD. Curr. Opin. Plant Biol. 9:

331-340.

Gläßgen, W.E. & Seitz, H.U. 1992. Acylation of anthocyanins with hydroxycinnamic acids via

1-O-acylglucosides by protein preparations from cell cultures of Daucus carota L. Planta 186:

582-585.

Hashimoto, F., Tanaka, M., Maeda, H., Fukuda, S., Shimizu, K. & Sakata, Y. 2002. Changes in flower

coloration and sepal anthocyanins of Cyanic delphinium cultivars during flowering. Biosci. Biotechnol.

Biochem. 66: 1652-1659.

Hause, B., Meyer, K., Viitanen, P.V., Chapple, C. & Strack, D. 2002. Immunolocalization of 1-O-sinapoylglucose:malate sinapoyltransferase in Arabidopsis thaliana. Planta 215: 26-32.

Matsuba, Y., Sasaki, N., Tera, M., Okamura, M., Abe, Y., Okamoto, E., Nakamura, H., Funabashi, H.,

Takatsu, M., Saito, M., Matsuoka, H., Nagasawa, K. & Ozeki, Y. 2010. A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium. Plant Cell 22: 3374-3389.

Nakayama, M., Koshioka, M., Yoshida, H., Kan, Y., Fukui, Y., Koike, A. & Yamaguchi, M. 2000. Cyclic

malyl anthocyanins in Dianthus caryophyllus. Phytochemistry 55: 937-939.

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In: Ashihara, H., Crozier, A. & Komamine, A. (eds.) Plant Metabolism and Biotechnology. pp. 321-342.

John Wiley & Sons, Ltd, Chichester.

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carotenoids. Plant J. 54: 733-749.

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of Anthocyanins. In: Kevin, G., Kevin, D. & Chris, W. (eds.) Anthocyanins. pp169-190. Springar

Science+Business, New York.

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structure to cell physiology. Nat. Prod. Rep. 26: 884-915.

ƹǩȖĸƣêɁ2:29˩ 2011 ˪

BSJ-Review 2:29 (2011)

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Master genes for nicotine biosynthesis in tobacco; approach using low-nicotine mutants

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Takayama 8916-5, Ikoma, Nara 630-0101, Japan

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�ʶŭȤů2ƤǵdzDZǖůĭǣǩąŵȮ (Gundlach et al. 1992, Blechert et al. 1995, Yukimune et al.

1996)1?�W��m�F,�Eāɓů?�E

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M.Y.Hiraiˬ1

V�|�r���MwN����Og�v�_h�

Ŕ¨� ÔɈ DZôĸȍȝŸ� ƹǩȖĸȍȝc�f�� °ʌ_ak�ɼƯh��

�230-0045� ǀǘŐ˥ɷõƨŖǷ 1-7-22

Functional identification of secondary metabolism-related genes by coexpression analysis Keywords: coexpression analysis; functional genomics; metabolomics; secondary metabolite; transcriptomics.

Masami Yokota Hirai

Metabolic Systems Research Team, RIKEN Plant Science Center 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

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