b supplementary fig s1. (a) zr75- and mcf7-pelp1 knockdown cells were generated as described in...
If you can't read please download the document
DESCRIPTION
p53 pS15-p53 DAPI PELP1 Merge Supplementary Fig. S3. ZR75 breast cancer cells cultured on glass coverslips were treated with campothecin (1 M) for 2hr. Cells were then fixed and co-stained with antibodies against PELP1 (red) and p53 (green), pS15 p53 (green), and analyzed by confocal microscopy. DAPI was used to localize the nucleus. Co-localization PELP1 and p53 can be seen by yellow color. DMSO campothecinTRANSCRIPT
B Supplementary Fig S1. (A) ZR75- and MCF7-PELP1 knockdown cells were generated as described in methods section. Pooled colonies were analyzed for PELP1 expression by western blot analysis. (B) MCF7 cells were transiently transfected with PELP1-siRNA or control siRNA. After 48h, cells were exposed to indicated concentration of campothecin and cell viability was measured using MTT assay. (C) ZR75 cells transiently transfected with PELP1-siRNA or control siRNA. After 48h, cells were exposed to radiation (2gy) and clonogenic (survival) assay was performed after 10 days. Survival fraction is expressed as percentage of colony formed in their respective untreated control group. p53 non functional model cells HEK293T cells (D), IOMM-LEE (E) expressing PELP1-specific shRNA or control shRNA were treated with indicated concentrations of Mitomycin C and clonogenic survival was assayed after 10 days. Error bar depicts standard error from triplicate experiments. *P