J ALLERGY CLIN IMMUNOL

VOLUME 133, NUMBER 2

Abstracts AB285

ESDAY

983 Effect Of Prenatal Exposure To Indoor PM2.5 andEnvironmental Tobacco Smoke Affecting Lower RespiratoryTract Infection Was Modified By ROS Genes: Cocoa Study

Dr. Song I. Yang, MD1, Dr. Eun Lee, MD1, Dr. Young Ho Jung, MD1,

Kil-Yong Choi2, Mi-Jin Kang, MS2, Ho-Sung Yu2, Cheol Min Lee3,

Prof. Youn Ho Shin, MD, PhD4, Prof. Kangmo Ahn, MD, PhD5,

Prof. Kyung Won Kim, MD, PhD6, Prof. Soo-Jong Hong, MD, PhD1

Cocoa study Group7; 1Childhood Asthma Atopy Center, Department of

Pediatrics, Asan Medical Center, University of Ulsan College of Medi-

cine, Seoul, South Korea, 2Asan Institute for Life Sciences, University

of Ulsan College of Medicine, Seoul, South Korea, 3Institute of Environ-

mental and Industrial Medicine, Hanyang University, Seoul, South Korea,4Department of Pediatrics, CHAMedical Center, CHAUniversity College

of Medicine, Seoul, South Korea, 5Department of Pediatrics, Samsung

Medical Center, Sungkyunkwan University School of Medicine, Seoul,

South Korea, 6Department of Pediatrics, Yonsei University College of

Medicine, Seoul, South Korea, 7COCOA study group.

RATIONALE: Prenatal air pollution exposure associates with impaired

fetal growth, decreased lung function, respiratory morbidity and mortality.

We aimed to investigate the influence of prenatal indoor fine particulate

matter (PM2.5) and environmental tobacco smoke (ETS) exposure and ge-

netic polymorphisms on the susceptibility to respiratory tract infection

(RTI) in infancy.

METHODS: Demographic, environmental factors and diagnosis of RTI in

the first year of life were evaluated in 231 infants from a birth cohort of

general population in Korea between 2007 and 2011. Indoor PM2.5 was

measured during the third trimester and mothers were asked about ETS

exposure during pregnancy. Nuclear factor (erythroid-derived 2)-like 2

(NRF2, rs6726395), glutathione S-transferase pi 1 (GSTP1, rs1695) and

glutathione S-transferase mu 1 (GSTM1, copy number variation) genotyp-

ing was performed.

RESULTS: Prenatal indoor PM2.5 or ETS exposure increased the risk of

bronchiolitis and lower RTI (LRTI), but not upper RTI (URTI), in infancy.

They also synergistically increased susceptibility to bronchiolitis and LRTI

(aOR 5.20; 95% CI 1.10-24.61, aOR 5.18; 95% CI 1.40-19.24, respec-

tively), but not URTI. This synergistic effect was increased by Nrf2 GG

(aOR10.23; 95%CI 1.02-102.57, aOR 14.01; 95%CI 1.42-138.02, respec-

tively) and GSTM1 null genotypes (aOR 9.45; 95% CI 1.03-86.78, aOR

5.70; 95% CI 1.02-31.95, respectively).

CONCLUSIONS: Indoor PM2.5 and ETS exposure during the prenatal

period synergistically increased susceptibility to bronchiolitis and LRTI,

but not URTI. This effect was modified by polymorphisms in reactive ox-

ygen species-related genes.

984 IgE Anti-Respiratory Syncytial Virus Antibodies In OlderAsthmatic Children

Mira Mandal1, Dr. Rauno O. Joks, MD, FAAAAI2, Dr. Kevin

Norowitz, MD3, Dr. Diana Weaver, MD4, Dr. Helen G. Durkin, PhD5,

Dr. Martin H. Bluth, MD, PhD6, Dr. Stephan Kohlhoff, MD5, Dr. Tamar

A. Smith-Norowitz, PhD7; 1SUNY Downstate Medical Center,2SUNY-HSC, Brooklyn, NY, 3SUNY Downstate Medical Center, Center

for Allergy and Asthma Research, Brooklyn, NY, 4SUNY Downstate

Med. Ctr, Brooklyn, NY, 5Center for Allergy and Asthma Research, State

University of New York Downstate Medical Center, Brooklyn, NY,6Wayne State University Medicine, Detroit, MI, 7Center for Allergy and

Asthma Research at SUNY Downstate, Brooklyn, NY.

RATIONALE: Respiratory syncytial virus (RSV) causes severe lower

respiratory tract disease in infant and young children, and is a serious

public health concern, as is the increase in pediatric asthma in the United

States. However, it remains unknown if IgE and RSVantibodies (Abs) play

a role in development of asthma.

METHODS: Total serum IgE, and IgE and IgG anti-RSVAb responses

were studied in older asthmatic compared with non asthmatic children (M/

F, mean age: 14) (N5 30, N543, respectively) (UniCAP total IgE

Fluoroenzymeimmunoassay (IU/mL), enzyme-linked immunosorbent

assay (U/mL; OD value).

RESULTS: Total serum IgE levels were increased 3-fold in asthmatic

compared with non asthmatic children (323 + 217, 107 + 116, P5 0.0003).

Further, IgE anti-RSV Ab levels were 1.5-fold higher in asthmatic

compared with nonasthmatic children (P5 0.01); IgG anti-RSVAb levels

were slightly increased in the asthmatic children (112 + 32, 81+ 59,

P50.003). Interestingly, values of IgE anti-RSVAbs positively correlated

with IgG anti-RSVAbs.

CONCLUSIONS: The presence of IgE anti-RSV abs in older children

further lends support to an important role of RSV infection in asthma.

985 Asthma Symptoms and Rhinovirus In A LongitudinalChildren's Cohort

Dr. Euan R. Tovey, PhD1,2, Dr. S. Stelzer-Braid, PhD3,4, Dr. B. G.

Toelle, PhD1, Ms. C. M. Willenborg, BSc3, Dr. H. K. Reddel, PhD,

MBBS1,2, Ms. F. L. Garden, MPH1,2, Prof. A. Jaffe, PhD, MD4,5, Ms. R.

Strachan5, Dr. B. G. Oliver, PhD1,2, Dr. Y. C. Belessis, MBBS4,5,

Prof. G. B. Marks, PhD, MD1,2, Prof. W. D. Rawlinson, PhD, MD3,4;1Woolcock Institute of Medical Research, Sydney, Australia, 2University

of Sydney, Australia, 3Virology Division, SEALS Microbiology, Prince

of Wales Hospital, Sydney, Australia, 4University of NSW, Australia,5Sydney Children’s Hospital, Randwick Australia.

RATIONALE: Emergency presentations for asthma are strongly associ-

ated with rhinovirus (HRV) infections in children. However, community

viral infections are common and often mild or asymptomatic, and the role

of viruses in changing daily symptoms is less clear. We hypothesized such

infections would be associated with increased symptoms.

METHODS: 67 children, aged 5-12, with moderately severe asthma, self-

collected nasal-wash and exhaled breath samples and recorded their recent

asthma and cold symptoms and current lung function, twice per week for

10 weeks. The presence of 8 viruses, including rhinovirus (HRV), was

analysed by PCR. A mixed model, to account for repeated measures, was

used to determine the current and delayed impact of viruses on symptoms

and lung function.

RESULTS: 25.5% of nasal samples and 11.5% of breath samples were

positive for HRV; only 1.8% were positive for other viruses. The presence

of HRVin nasal wash, but not in breath, was associatedwith the presence of

symptoms; adjusted odds ratios: cough 2.53, (95%CI 1.62-3.94), wheeze

3.05, (95%CI 1.89 to 4.93), self-reported febrile 2.07, (95%CI 1.17 to 3.64)

and coryzal symptoms 1.95, (95%CI 1.14 to 3.32) over the previous 3-4

days. These associations remained 3-4 days later, but generally not 7 days

later. There was no association between any virus positivity and changes in

electronically recorded PEFR and FEV1.

CONCLUSIONS: In a longitudinal study of children with moderate

asthma, the presence of rhinovirus in nasal wash, but not in exhaled breath,

was associated with worsening of several indices of respiratory symptoms

for up to a week.

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