Asthma Symptoms and Rhinovirus In A Longitudinal Children's Cohort

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<ul><li><p>Fluoroenzymeimmunoassay (IU/mL), enzyme-linked immunosorbent</p><p>assay (U/mL; OD value).</p><p>RESULTS: Total serum IgE levels were increased 3-fold in asthmatic</p><p>compared with non asthmatic children (323 + 217, 107 + 116, P5 0.0003).Further, IgE anti-RSV Ab levels were 1.5-fold higher in asthmatic</p><p>compared with nonasthmatic children (P5 0.01); IgG anti-RSVAb levelswere slightly increased in the asthmatic children (112 + 32, 81+ 59,</p><p>P50.003). Interestingly, values of IgE anti-RSVAbs positively correlatedwith IgG anti-RSVAbs.</p><p>CONCLUSIONS: The presence of IgE anti-RSV abs in older children</p><p>further lends support to an important role of RSV infection in asthma.</p><p>985 Asthma Symptoms and Rhinovirus In A LongitudinalChildren's CohortDr. Euan R. Tovey, PhD1,2, Dr. S. Stelzer-Braid, PhD3,4, Dr. B. G.</p><p>Toelle, PhD1, Ms. C. M. Willenborg, BSc3, Dr. H. K. Reddel, PhD,1,2 1,2 4,5</p><p>J ALLERGY CLIN IMMUNOL</p><p>VOLUME 133, NUMBER 2</p><p>Abstracts AB285</p><p>UESDAY983 Effect Of Prenatal Exposure To Indoor PM2.5 andEnvironmental Tobacco Smoke Affecting Lower RespiratoryTract Infection Was Modified By ROS Genes: Cocoa Study</p><p>Dr. Song I. Yang, MD1, Dr. Eun Lee, MD1, Dr. Young Ho Jung, MD1,</p><p>Kil-Yong Choi2, Mi-Jin Kang, MS2, Ho-Sung Yu2, Cheol Min Lee3,</p><p>Prof. Youn Ho Shin, MD, PhD4, Prof. Kangmo Ahn, MD, PhD5,</p><p>Prof. Kyung Won Kim, MD, PhD6, Prof. Soo-Jong Hong, MD, PhD1</p><p>Cocoa study Group7; 1Childhood Asthma Atopy Center, Department of</p><p>Pediatrics, Asan Medical Center, University of Ulsan College of Medi-</p><p>cine, Seoul, South Korea, 2Asan Institute for Life Sciences, University</p><p>of Ulsan College of Medicine, Seoul, South Korea, 3Institute of Environ-</p><p>mental and Industrial Medicine, Hanyang University, Seoul, South Korea,4Department of Pediatrics, CHAMedical Center, CHAUniversity College</p><p>of Medicine, Seoul, South Korea, 5Department of Pediatrics, Samsung</p><p>Medical Center, Sungkyunkwan University School of Medicine, Seoul,</p><p>South Korea, 6Department of Pediatrics, Yonsei University College of</p><p>Medicine, Seoul, South Korea, 7COCOA study group.</p><p>RATIONALE: Prenatal air pollution exposure associates with impaired</p><p>fetal growth, decreased lung function, respiratory morbidity and mortality.</p><p>We aimed to investigate the influence of prenatal indoor fine particulate</p><p>matter (PM2.5) and environmental tobacco smoke (ETS) exposure and ge-</p><p>netic polymorphisms on the susceptibility to respiratory tract infection</p><p>(RTI) in infancy.</p><p>METHODS: Demographic, environmental factors and diagnosis of RTI in</p><p>the first year of life were evaluated in 231 infants from a birth cohort of</p><p>general population in Korea between 2007 and 2011. Indoor PM2.5 was</p><p>measured during the third trimester and mothers were asked about ETS</p><p>exposure during pregnancy. Nuclear factor (erythroid-derived 2)-like 2</p><p>(NRF2, rs6726395), glutathione S-transferase pi 1 (GSTP1, rs1695) and</p><p>glutathione S-transferase mu 1 (GSTM1, copy number variation) genotyp-</p><p>ing was performed.</p><p>RESULTS: Prenatal indoor PM2.5 or ETS exposure increased the risk of</p><p>bronchiolitis and lower RTI (LRTI), but not upper RTI (URTI), in infancy.</p><p>They also synergistically increased susceptibility to bronchiolitis and LRTI</p><p>(aOR 5.20; 95% CI 1.10-24.61, aOR 5.18; 95% CI 1.40-19.24, respec-</p><p>tively), but not URTI. This synergistic effect was increased by Nrf2 GG</p><p>(aOR10.23; 95%CI 1.02-102.57, aOR 14.01; 95%CI 1.42-138.02, respec-</p><p>tively) and GSTM1 null genotypes (aOR 9.45; 95% CI 1.03-86.78, aOR5.70; 95% CI 1.02-31.95, respectively).</p><p>CONCLUSIONS: Indoor PM2.5 and ETS exposure during the prenatal</p><p>period synergistically increased susceptibility to bronchiolitis and LRTI,</p><p>but not URTI. This effect was modified by polymorphisms in reactive ox-</p><p>ygen species-related genes.</p><p>984 IgE Anti-Respiratory Syncytial Virus Antibodies In OlderAsthmatic ChildrenMira Mandal1, Dr. Rauno O. Joks, MD, FAAAAI2, Dr. Kevin</p><p>Norowitz, MD3, Dr. Diana Weaver, MD4, Dr. Helen G. Durkin, PhD5,</p><p>Dr. Martin H. Bluth, MD, PhD6, Dr. Stephan Kohlhoff, MD5, Dr. Tamar</p><p>A. Smith-Norowitz, PhD7; 1SUNY Downstate Medical Center,2SUNY-HSC, Brooklyn, NY, 3SUNY Downstate Medical Center, Center</p><p>for Allergy and Asthma Research, Brooklyn, NY, 4SUNY Downstate</p><p>Med. Ctr, Brooklyn, NY, 5Center for Allergy and Asthma Research, State</p><p>University of New York Downstate Medical Center, Brooklyn, NY,6Wayne State University Medicine, Detroit, MI, 7Center for Allergy and</p><p>Asthma Research at SUNY Downstate, Brooklyn, NY.</p><p>RATIONALE: Respiratory syncytial virus (RSV) causes severe lower</p><p>respiratory tract disease in infant and young children, and is a serious</p><p>public health concern, as is the increase in pediatric asthma in the United</p><p>States. However, it remains unknown if IgE and RSVantibodies (Abs) play</p><p>a role in development of asthma.</p><p>METHODS: Total serum IgE, and IgE and IgG anti-RSVAb responses</p><p>were studied in older asthmatic compared with non asthmatic children (M/</p><p>F, mean age: 14) (N5 30, N543, respectively) (UniCAP total IgEMBBS , Ms. F. L. Garden, MPH , Prof. A. Jaffe, PhD, MD , Ms. R.</p><p>Strachan5, Dr. B. G. Oliver, PhD1,2, Dr. Y. C. Belessis, MBBS4,5,</p><p>Prof. G. B. Marks, PhD, MD1,2, Prof. W. D. Rawlinson, PhD, MD3,4;1Woolcock Institute of Medical Research, Sydney, Australia, 2University</p><p>of Sydney, Australia, 3Virology Division, SEALS Microbiology, Prince</p><p>of Wales Hospital, Sydney, Australia, 4University of NSW, Australia,5Sydney Childrens Hospital, Randwick Australia.</p><p>RATIONALE: Emergency presentations for asthma are strongly associ-</p><p>ated with rhinovirus (HRV) infections in children. However, community</p><p>viral infections are common and often mild or asymptomatic, and the role</p><p>of viruses in changing daily symptoms is less clear. We hypothesized such</p><p>infections would be associated with increased symptoms.</p><p>METHODS: 67 children, aged 5-12, with moderately severe asthma, self-</p><p>collected nasal-wash and exhaled breath samples and recorded their recent</p><p>asthma and cold symptoms and current lung function, twice per week for</p><p>10 weeks. The presence of 8 viruses, including rhinovirus (HRV), was</p><p>analysed by PCR. A mixed model, to account for repeated measures, was</p><p>used to determine the current and delayed impact of viruses on symptoms</p><p>and lung function.</p><p>RESULTS: 25.5% of nasal samples and 11.5% of breath samples were</p><p>positive for HRV; only 1.8% were positive for other viruses. The presence</p><p>of HRVin nasal wash, but not in breath, was associatedwith the presence of</p><p>symptoms; adjusted odds ratios: cough 2.53, (95%CI 1.62-3.94), wheeze</p><p>3.05, (95%CI 1.89 to 4.93), self-reported febrile 2.07, (95%CI 1.17 to 3.64)</p><p>and coryzal symptoms 1.95, (95%CI 1.14 to 3.32) over the previous 3-4</p><p>days. These associations remained 3-4 days later, but generally not 7 days</p><p>later. There was no association between any virus positivity and changes in</p><p>electronically recorded PEFR and FEV1.</p><p>CONCLUSIONS: In a longitudinal study of children with moderate</p><p>asthma, the presence of rhinovirus in nasal wash, but not in exhaled breath,</p><p>was associated with worsening of several indices of respiratory symptoms</p><p>for up to a week.T</p></li></ul>