Fig.S1 Putative cis-regulatory elements in promoter region of MdATG10, as predicted from PlantCARE database. Letters in color indicates the coding sequence of MdATG10.

Fig.S2 PCR confirmation of pro-MdATG10-GUS transferred Nicotiana plants. Lanes: M, molecular marker DL2000; V, positive vector containing pGWB433-MdATG10pro plasmid; WT, non-transformed wild-type; P-10 and -17, MdATG10pro-transgenic tobacco lines.

Supplemental Table1 Predicted cis-regulatory elements with putative functions identified in promoter of MdATG10 using PlantCARE database.

Cis-element Positions Sequences Putative functionARE 678 (-) AAACCA cis-acting regulatory element

essential for the anaerobic induction

1142 (+) AAACCA

Box 4 1288 (-) ATTAAT part of a conserved DNA module involved in light responsiveness1306 (-) ATTAAT

1302 (-) ATTAATBox II 1101 (+) TGGTAATAA part of a light responsive elementCAT-box 477 (+) GCCACT cis-acting regulatory element

related to meristem expressionCCAT-box 467 (+) CAACGG

MYBHv1 binding site1818 (-) CAACGG514 (+) CAACGG

G-box 1694 (+) CACGAC cis-acting regulatory element involved in light responsiveness1795 (+) CACGAC

MYB-r 467 (-) CCGTTGMYB recognition site1818 (+) CCGTTG

514 (-) CCGTTGMyb-b 1824 (+) CAACAG Myb-binding siteO2-site 320 (+) GATGATGTGG cis-acting regulatory element

involved in metabolism regulationTATC-box 1391 (-) CAACTG cis-acting element involved in

gibberellin-responsivenessTC-rich 1103 (-) GTTTTCTTAC cis-acting element involved in

defense and stress responsiveness1530 (-) GTTTTCTTACTCA-element 671 (-) CCATCTTTTT

cis-acting element involved in salicylic acid responsiveness

1133 (-) TCAGAAGAGG902 (+) TCAGAAGAGG1639 (+) CCATCTTTTT

TCCC-motif 585 (+) TCTCCCT part of a light responsive elementTCT-motif 97 (+) TCTTAC part of a light responsive element652 (+) TCTTACTGA-element 1777 (+) AACGAC auxin-responsive element

Supplemental Table2 Primers used in this study

Gene Sequence (5'-3') PurposeATG10 F: ATGGAGGTTTTGGGATGGGAT cloning Sequence

R: TCAATTACTAGTTTTATGATGATTC

sATG10 F: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGAGGTTTTGGGATGGGA

Vector construction for sub-cellular localization

R: GGGGACCACTTTGTACAAGAAAGCTGGGTTATTACTAGTTTTATGATGAT

ATG10pro F: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCCCTAGAATGAGGAACAGTA

Vector construction for the pro-MdATG10-GUS staining

R: GGGGACCACTTTGTACAAGAAAGCTGGGTTGATATCCGCTTGATTTCCTT

oeATG10 F: CGGGATCCATGGAGGTTTTGGGATG Vector construction for plant transformationR: GGGGTACCATTACTAGTTTTATGATGATTCAAC

dATG10 F: GAGAACACGGGGGACTCTAGA DNA confirmation of MdATG10 for transgenic identificationR: CGATCGGGGAAATTCGAGCTC

qATG10 F: GGTTTTGGGATGGGATGGA Quantitative expression of MdATG10R: AGAGATGAGGAGGCTGTTTG

MDH F: CGTGATTGGGTACTTGGAAC Reference gene used in real-time PCRR: TGGCAAGTGACTGGGAATGA

qSOS1 F: AGGAAACCATGAAATTGTGTGG Quantitative expression of MdSOS1R: GATCATGTCACAAATGTAGGGC

qSOS2 F: AATCAATGGGTCTCAAGGTC Quantitative expression of MdSOS2R: CCTCCTTCGGTTTCCAAATA

qSOS3 F: GGGGTTATTGAGTTTGGAGA Quantitative expression of MdSOS3R: GGATGCTTCGACACAAATTC

qNHX1 F: AAGCGACAGTCCTGGAACAT Quantitative expression of MdNHX1R: TATTATCACTTGCTGCCGGAG

qHKT1 F: TCGTTCGCTATTTCGTGTCCT Quantitative expression of MdHKT1R: TGGGCCTGAAAGAAGTGTTTG

qATG3a F: AAGGGGGCGGAGATGGTTC Quantitative expression of MdATG3aR: GCACTTAGAGACGAGGTTATCGC

qATG3b F: AGGGAGATGGTTTTGAAACAGA Quantitative expression of MdATG3bR: ACTTAGAGACGAGGTTATCGC

qATG5 F: GCAGGTCGTGTTCCAGTTC Quantitative expression of MdATG5R: CCTCCTCCTCCTTGTATCTCAA

qATG7a F: GCGGATATGAGCAACCTTGGC Quantitative expression of MdATG7aR: ATCAATAGGCGCAACGACATCA

qATG7b F: ATCGGTAACAGGAGTAAGTCGG Quantitative expression of MdATG7bR: TTTATCAAGCGCATGAAAGCCT

qATG8c F: GCGTTCAAGATGGAGCACCCTC Quantitative expression of MdATG8cR: CAGCCCTTTCCACAACCACTGG

qATG8f F: TCGTAGACAATGTCCTCCCAGC Quantitative expression of MdATG8fR: CCAAATGTGTTCTCGCCACTGT

qATG8i F: GCAGCAGGCTTCACTTGACTCC Quantitative expression of MdATG8iR: GGAATCCATGCGACTGGCTGTT

qATG18a F: ATGATTCCAGGCTTGCCTGCTTTG Quantitative expression of MdATG18aR: TGCAGCAAAGTTCCGTCGAGAGTA