areedpresentation
TRANSCRIPT
THE PREPARATION OF
Alys Reed Biological Engineering Major Louisiana State University Mentor: Dr. Ram Devireddy LA-SIGMA REU Summer 2012
Artificially prepared vesicle
Concentric bilayer phospholipid structures enclosing aqueous core
Lipid chains with surfactant properties
Surface ligands
Amphiphilic Contains both
hydrophilic and lipophilic components
Hydrophilic head Alcohol and Phosphate
group Hydrophobic tail Long fatty acid
hydrocarbon chains
Distinguished by number of lamellae and size
Small unilamellar vesicles – SUV
Large unilamellar
vesicles- LUV Multilamellar vesicles –
MLV Multivesicular vesicles -
MVV
SUV
Smaller than 50 nm LUV Larger than 50 nm
MVV 2,000 – 40,000 nm
GUV 10,000 – 10,000,000 nm
MLV
500 nm to 10,000 nm
Administration of nutrients and pharmaceutical
Vaccines
Vitamins and Dietary supplements
Delivery of dyes to textiles
Pesticides to plants
Enzymes and nutritional supplements to foods
Cosmetics to skin
Model for artificial cells
WHY THE HEATING APPROACH?
Simple and time efficient No organic solvent
residues Sterilization not required Stable product Large scale production
CONS
Population heterogeneous
SCANNING ELECTRON MICROSCOPE- SEM
Scans sample with a beam of electrons in a raster scan pattern
TRANSMISSION ELECTRON MICROSCOPE-TEM
Beam of electrons transmitted through ultra thin specimen