annual report 2010-2011 - nutrahelix dr. v prakash, former director, central food technological...
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Corporate Information
Board of Directors (As on 1st October, 2010) Dr. Suman Preet Singh Khanuja Chief Mentor & Director
Mr. Shreenarayan Agrawal Chief Operator & Director
Mrs. Meena Agrawal Director - Corporate Social Responsibility
Mr. Nandan Agrawal Director - Business Development
Head Office 19-A Sita Estate, Aziz Baug
Mahul Road, Chembur
Mumbai 400074, India
Phone: +91 22 25545244
Fax: +91 22 25545864
Research Centre Knowledge Centre D/365, Nandan Impex Building, 101, Park View Apartments,
MIDC Turbhe, Building No.II,
Navi Mumbai 400705, Mandi Village Road, Mehrauli,
Maharashtra, India New Delhi 110030, India
Phone: +91 22 27614451 Phone: +91 11 26804923
Fax: +91 22 27614451 Fax: +91 11 26804923
Website www.nutrahelix.com
A word from Chief Mentor…
“The beginning of knowledge is the discovery of something we do not understand’’, was stated by the famous American author Frank Herbert, well known for his imagination in science. Today it seems true that food is something that we do not know well from the appropriateness perspective and hence it needs to be rediscovered. NutraHelix represents a unique industrial platform that was founded to create a discovery niche for future foods that would be able to enhance quality of life. That is why the mission slogan ‘making health sense in foods’ defines it. We began humbly through a science and business partnership in Mumbai
towards the end of 2009 as the foundation for Research Centre and also began the New Year with Knowledge Centre emerging with the ringing bells of 2010. The state-of-art laboratories at the Research Centre, Mumbai were launched on 16th February, 2011 by Dr. V Prakash, the torch bearer of food technology in the country and globe. NutraHelix Biotech Pvt. Ltd. has been initiated as the translational research venture aiming to learn from nature about nature for creation of knowledge based natural ingredients for functional foods with thorough scientific validation. It will not be inappropriate to say that the second ‘Green Revolution’ will happen only through quality of food and not by quantities. Linking agriculture with health sector is feasible when a strategic value chain is planned for meeting the nutritional requirements of life by identifying the essential health requirements of human body. NutraHelix has taken care of this aspect holistically while creating the infrastructure along with matching and competent scientific expertise. The projects that have begun, along with those planned for immediate future cover the aspects of bioprospecting, synbiotics, high value lipids, proteins and carbohydrates, food matrices, functional foods, nutraceutical and bioactive phytochemicals, enzymes, polysaccharides, biomolecules, food quality and safety standards. The skilled scientific team has blend of young and experienced members with sound training in field of agriculture, food science, microbiology, molecular biology, pharmaceutical science, nutrition, chemical engineering, formulation, product development and processing. Further to strengthen the ability of the organization, the management is represented by the competent Board of Directors namely Mr. Shreenarayan Agrawal (Industrial Business), Mrs. Meena Agrawal (Corporate Social Responsibility), Mr. Nandan Agrawal (Chemical Engineer with MBA) and lastly me (Science and Technology) as the base. The whole team and organization is committed on its mission of enhancing quality of life through science. This is the formative year but significant progress is visible from the first Annual Report itself which is an indicator that we will be the change that everyone wants to see in the world for health through foods and nature. With grateful thanks to all, I am happy to place the very first progress report to our catalysts, collaborators, promoters and clients for their knowledge inputs towards the build-up of NutraHelix
Dr. Suman Preet Singh Khanuja
Acknowledgement
Dr. V Prakash, Former Director, Central Food Technological Research
Institute (CFTRI/CSIR), Mysore, India
Director and Team, Indian Agricultural Research Institute (IARI/ICAR), New
Delhi, India
Director and Team, Central Institute of Post Harvest Engineering &
Technology (CIPHET/ICAR), Ludhiana, India
President, Director and Team, Indian Institute of Advanced Research (IIAR),
Gandhinagar, India
Co-Directors and Team, Joint Genomic Center (JGC), Sofia, Bulgaria
Director and Team, Centre for Advanced Research & Training in Biosciences
(CARTiB), Mumbai, India
Chairman and Team, Nandan Group, Mumbai, India
CONTENTS
Executive Summary
Organizational structure
Company Expertise
Research and Technology Areas
Target Products
Scientific Expertise
Projects in operation
Projects planned for immediate future
Infrastructure
Specialized Facilities
Linkages
Publications
Expert Services
Participation in National and International Events
Lead Lectures and Keynote Addresses by Chief Mentor
NutraHelix in Pictures
Statement of Assets and Costs
Executive Summary
Nutraceuticals are technically defined as foods or food derived substances in
extracted form, which carry the capability or ingredients to provide medicinal and
health benefits to living beings. Nutraceuticals may range from isolated nutrients,
herbal products, dietary supplements and diets to engineered “designer” foods and
processed products such as cereals, soups and beverages. Doubtlessly, many of these
products possess pertinent physiological functions and valuable biological activities.
Nutraceuticals are now rapidly emerging through scientific research with such leads
being generated all over the world. In many cases nutraceuticals offer an advantage
over the synthetic drugs under development by the pharmaceuticals industry. These
food derived pharmacological activities are making possible clinical use and thus
helping in disease prevention as well as therapeutics in several ailments and health
disorders. With availability of vast Indian knowledge of local health traditions and
food habits of communities, targeted R&D on their biological validation and quality
creates a great scope for novel functional foods, nutraceuticals and health
supplements from crops, plants and microflora.
India has all the advantages and potential of becoming the nutraceutical hub with
a long lasting tradition of maintaining health by the intake of appropriate food and
nutrition. India is home to almost all kinds of plants ranging from tropical, sub-
tropical and temperate zone plants. Moreover, India is blessed with abundant herbs;
the extracts of many of these herbs have been standardised and tested clinically for
its quality and efficacy. These extracts form an integral part of nutraceuticals and
functional foods that are available in developed countries. Additionally, India has a
lot of other advantages like qualified human resources, world class R & D facilities
and varied raw material-aspects that give our country a leading edge. Further, the
advantage of knowledge based remedies gives India tremendous leads in finding
newer applications (because of Ayurveda). Ayurveda, the five thousand year old
ancient Indian health science have mentioned benefits of food for therapeutic
purpose. Documents clearly show that the medicinal benefits of food have been
explored for thousands of years. This scenario puts India an advantage of Science
driven novel foods for health.
In this backdrop, NutraHelix Biotech Pvt. Ltd. was incorporated on 21st
December, 2009 as a result of joint initiative by Dr. Suman Preet Singh Khanuja and
Mr. Shree Narayan Agrawal on a Science and Business partnership platform as a
unique start up industry in the nutraceutical sector. Dr. Khanuja being former
Director of Central Institute of Medicinal and Aromatic Plants (CIMAP/CSIR),
Lucknow, India brings almost three decades of R&D experience and technology
application in the field of agriculture, biotechnology and natural products with IP
management. Parallel to it, Mr. Agrawal carries with him a rich industrial experience
from manufacturing to training in petrochemical sector involving import-exports in
global scenario. Dr. Khanuja was requested to lead and mentor this venture as R&D
based industry for creation of novel products, patents (IP) and technologies in the
nutraceutical and functional food sector to be translated into a real time business by
the expertise of Mr. Agrawal. Consequently, Dr. Khanuja has been leading
NutraHelix Biotech Pvt. Ltd. as its Chief Mentor with Mr. Agrawal leading as Chief
Operator since January, 2010. Nandan Group offered research labs available in their
building and hence research work on lead generation aspects began in February,
2010 itself. Further synergy was created by taking incubator space for food
microbiology work at Indian Agricultural Research Institute (IARI/ICAR), New
Delhi in October, 2010. This enhanced the pace of lead generation particularly pre-
and pro-biotics domains. Chief Mentor has been bringing visibility to the project by
delivering lead lectures in National Events like Nutra India Summit (2010, 2011);
Brainstorming sessions in National Academy of Agricultural Sciences (2010) and
Indian Horticultural Congress (2010) on the scope and opportunity aspects that have
been conceptualized by NutraHelix Biotech Pvt. Ltd. for the discovery platform on a
R2B mode (Research to Business).
Simultaneously, a state-of-art Research Centre with equipped labs was developed
on a leased out space covering more than 3000 square feet area at Turbhe in Navi
Mumbai. Launching of full-fledged Research Centre took place during 6th
Nutra
India Summit on 16th
February, 2011 by Dr. V. Prakash (then Director of Central
Food Technological Research Institute, CFTRI/CSIR, India). Today, NutraHelix
Biotech Pvt. Ltd. has full Research Centre with laboratories in Navi Mumbai and
Knowledge Centre in New Delhi. It is now developing Processing set-up for
translating the research leads from laboratories into manufacturing arena. This
venture has been an example of unique combination of Knowledge equity (Science)
and Financial equity (Business) on platform.
NutraHelix Biotech Pvt. Ltd. aims to develop well validated technologies for
functional foods and nutraceuticals as the sustainable IP and affordable healthcare
products, and its efforts in the area of research and development are directed to
achieve this goal. There is a dedicated Research team of scientists and associates for
developing nutraceuticals, which will have a tremendous potential for growth due to
the major factors such as ageing population, changing lifestyle, increasing medical
costs, high spending power, awareness of preventive medicine, and increasing
malnutrition led diseases. The Company has plans to further strengthen the R&D
base to cater to more healthcare needs and expanding niches in all nutraceutical
segments, both in domestic as well as international markets.
Organizational structure
Board of Directors (BOD)
Chief Operator Chief Mentor
Director-Business Development
Research & Development Process/Product Development Analytical Testing Business Development & Operations
Lead Scientist Lead Scientist Quality Manager Information Officer Business Manager
(Research Labs) (Process Technology) (Testing Labs) (Knowledge Centre) (Head Office)
Scientists & Associates Scientists & Associates Scientists & Associates
Industrial Microbiology Chemical engineering Analytical Chemist
Food Science & Technology Food &Nutrition Formulations/Pharmacy Assistants Assistants*
Biochemistry Post Harvest Technology* Dietician (F&N) (Office/Technical) (Office/Business)
Natural Prod Chemistry* Industrial Biotech* Microbiology (Med)*
Molecular Biology* Food Technology* Pharmacology/Toxicology*
Lab Technicians Plant Attendants Lab Technicians Liaison Staff Support Staff
Trainees/Fellows** Trainees/Assistants** Trainees/Assistants** Trainees** Trainees**
*To be appointed in due course, rests are already in place
**Partially filled, some vacancies to be filled later
Company Expertise
Agriculture, Food & Nutrition
Biotechnology (Plant/Animal/Microbial)
Biochemistry & Molecular Biology
Botany & Plant Sciences
Bio-Prospection & Conservation
Cell & Tissue Culture
Food Science & Technology
Medicinal & Aromatic Plants and Metabolomics
Food Processing & Product Development
Chemical Engineering & Pilot Plants
Research and Technology Areas
Food Science and Technology
Biochemistry and Nutrition
Food Quality Assays and Bio-testing
Biotechnology and Molecular Biology (Plant and Microbial)
Bio-prospecting & Conservation (Higher and Lower Plants)
Plant Metabolomics
Plant Tissue Culture & In vitro production
Microbiology and Algal technology
Botany and Pharmacognosy (Medicinal and Nutraceutical Plants)
Food ingredients (Nutraceuticals) and Supplements
Post-harvest technology
Food Processing and Product Development
Target Products
Novel Food Matrixes
Pre- & Pro-biotics
Dietary & Soluble Fibres
Antioxidants & Immunity Boosters
Lipids & Carbohydrates for Health
Millet Based Formulations
Gluten Free Formulations
Health Ingredients for Fast Foods
Health Beverage Formulations
Functional Foods for Liver & Cardiovascular Health
Metabolism Promoters & Gut Functioning Aids
Microbial & Enzymatic Supplements
Medicinal Plants based Phytoceuticals & Bioactives
Flavour & Fragrance of Nutraceutical Values
Scientific Expertise
Name Designation Specialization
Dr. Jai Shanker Arya
Lead Scientist Biotechnology
Industrial Microbiology
Plant bioactives and products
Bioreactors and Fermentation
Mr. Dharmendra Jain
Lead Scientist Process Technology (Pharma)
Chemical Engineering
Pilot and Manufacturing Plants
Fermentation Technology
Ms. Ashima Tewani*
Associate Scientist
Food Science and Technology
Food Microbiology
Food Analysis and Formulation
Mr. Yashaswi Singh
Scientist Trainee Plant Sciences
Biotechnology and Molecular Biology
Biochemistry
Mr. Anil Rambadan
Rajbhar
Research Associate Plant Taxonomy
Botany and Pharmacognosy
Biodiversity Survey
Herbaria and Repository
Ms. Sonali Avinash Dolas Research Associate Food Science and Nutrition
Dietetics
Home Science Applications (Foods)
Ms. Disha Gosalia Research Trainee Food Science and Nutrition
Dietetics
Home Science Applications (Foods)
Mr. Tirupati Babulal
Tatewar**
Associate Scientist Applied Chemistry
Analytical Instrumentation
Quality Testing (Natural products)
Mr. Jatinder Singh**
Technology
information Officer
(Scientist)
Biotechnology
Molecular Biology
Biochemical Technology
Pharmaceutical Sciences
Mr. Chandan Prakash** Technical Quality
Manager
(Scientist)
Business Administration
Pharmaceutical Sciences
Botany and Biotechnology
Formulation and Manufacturing
* Scientist Trainee till 28th
February, 2011 and Associate Scientist w.e.f. 1st
March, 2011
**To be shortly joining
Projects in operation
1. Bioprospection, documentation and repository of traditionally used plants
and foods
New research conducted among food scientists show that there is more to food
science than what was understood just a couple decades ago. Until just recently,
analysis of food was limited to the flavour of food (sensory taste and texture) and its
nutritional value (composition of carbohydrates, fats, proteins, water, vitamins and
minerals). However, there is growing evidence that other components of food may
play an integral role in the link between food and health. These chemical components
are derived from plant, food, and microbial sources, and provide medicinal benefits
valuable to long-term health. Examples of these nutraceutical chemicals include
probiotics, antioxidants, and phytochemicals. Nutraceutical products were considered
alternative medicine for many years. Nutraceuticals have become a more mainstream
supplement to the diet, now that research has begun to show evidence. India has all the
advantages of becoming a nutraceutical hub. India has a long lasting tradition of
maintaining health by the intake of appropriate food and nutrition. India is home to
almost all kinds of plants ranging from tropical, sub-tropical and temperate zone
plants. India is blessed with abundant herbs; the extracts of many of these herbs have
been standardised and tested clinically for its quality and efficacy. These extracts form
an integral part of nutraceuticals and functional foods that are available in developed
countries. Garcinia cambogia, Gymnema sylvestre, Phyllanthus embelica, Tribulus
terrestris, Momordica charantia, Curcuma longum, Green tea, Adathoda vasica,
Andrographis, Ginger, Tulsi, Phyllanthus niruri are quite a few of the original Indian
botanical products that are quite popular worldwide. These are used in a variety of
nutraceutical and functional food products. The world market for such products is ever
growing. Such products are being launched in India too. In fact, the use of these
products by the Indian consumers has been through the prescription channel. Some
examples of natural products used and exported from centuries in India are Aloe,
Green Tea, Withania, Chamomile, Ginger, Turmeric, Saffron, and Brahmi. Therefore,
this project aims at bioprospecting biodiversity and documenting the same while
creating real-time repository in and digital formats with following main objectives:
i. Collection of seeds and plant parts and reference food repository development
ii. Herbaria preparation and storage (real time and digitised)
iii. Monographs development and newsletter release
iv. Publications, Reports, Whitepapers, Brochure and Website/Digital Products
The collection has begun with weed species from road side, agricultural fields and
wild habitats without affecting the conservation status of the species. This will follow
their chemical/ biological analysis for food/ nutra values. The current status is as
follows:
Number of plant sources for which seeds are available: 70
Number of plant sources for which herbaria are available: 13
Number of plant sources for which both seeds and herbaria are available: 26
2. Novel synbiotic products development and process optimization.
Probiotics are referred to microflora consisting mainly the bacteria that help
maintain the natural balance of microflora in the human/ animal intestines. The normal
human digestive tract contains about 400 types of probiotic bacteria that as one of the
benefits reduce the growth of harmful bacteria and promote a healthy digestive
system. Experiments into the benefits of probiotic therapies suggest a range of
potentially beneficial medicinal uses for probiotics. An increased demand for nondairy
probiotic products comes from vegetarianism, milk cholesterol content, and lactose
intolerance. Therefore, the development of these products is a key research priority for
food design and a challenge for both industry and science sectors. Most of the
available products are some form of dairy, such as milk, ice cream, yogurt, cheese,
and frozen desserts, despite the continuously growth of the nondairy sector, with
products like soy-based drinks, fruit-based foods, and other cereal-based products.
In contrast prebiotics are non-digestible oligosaccharides particularly fructo-
oligosaccharides are known to stimulate the growth of endogenous and exogenous
bacteria into the gut. Combination of both probiotics and prebiotics known as
synbiotics represent the ultimate beneficial role into the host system. Still hardly any
non-dairy probiotic products are available which are stable on ambient temperature in
the market, it offers an R&D challenge for scientists to develop such stable non-dairy
products at ambient temperature. With this rationale, the project “Novel synbiotic
products development and process optimization” was taken up to develop stable
products with these main objectives:
i. Isolation and characterization of probiotic microflora from natural sources.
ii. Identification of prebiotic candidate materials from edible wastes of crops.
iii. Fermentation process for large scale probiotic cultures and stable synbiotic
formulations.
Around 52 strains were isolated from edible fermented food preparations involving
cereals, millets, fruits, vegetables and milk derivatives as follows:
S. No. Fermented preparation Number of Isolated
Probiotic strains
1 Fermented Dough (Cereals
and Millets)
27
2 Pickles (Fruits/ Vegetables) 4
3 Kimchi 3
4 Milk curd/Yoghurt 18
Biochemical Characterization of Isolates
S. No. Biochemical Characterization
Number of Positive Isolates
1 Intrinsic Antibiotic Resistance Pattern
(Ampicillin, Tetracycline, Rifampicin,
Ciprofloxacin, Gentamycin,
Erythromycin, Streptomycin,
Vancomycin, Penicillin, Nalidixic acid,
Linezoid, Kanamycin and Amoxicillin)
5 isolates were found to be resistant
against all tested antibiotics. All
resistant 5 isolates showed minimum
one positive character of probiotics
2 Bacteriocin production against E. coli, B.
Subtilis and P. aeruginosa
6 isolates showed antibacterial activity
against Escherichia coli
14 isolates showed antibacterial activity
against Bacillus subtilis
3 Lactose utilising capacity 11 isolates showed Lactose utilising
capacity
4 Proteolytic activity 25 isolates showed Proteolytic activity
5 Amylolytic activity 18 isolates showed Amylolytic activity
6 Lipolytic activity 20 isolates showed Lipolytic activity
3. Plant based sourcing for high value lipids, proteins and carbohydrates
Human beings evolved consuming a diet that contained approximately equal
amounts of omega-6 and omega-3 essential fatty acids. Over the past 100–150 years
there has been an enormous increase in the consumption of omega-6 fatty acids due to
the increased intake of vegetable oils from seeds of corn, sunflower, safflower, cotton
and soybeans. Today, in Western diets, the ratio of omega-6 to omega-3 fatty acids
ranges from 10 to 20:1 instead of the traditional range of 1–2:1. Studies indicate that a
high intake of omega-6 fatty acids shifts the physiologic state to one that is pro-
thrombotic and pro-aggregatory, characterized by increases in blood viscosity,
vasospasm, and vasoconstriction and decreases in bleeding time, whereas omega-3
fatty acids have anti-inflammatory, antithrombotic, anti-arrhythmic, hypolipidemic,
and vasodilatory properties. These beneficial effects of omega-3 fatty acids have been
shown in the secondary prevention of coronary heart disease and hypertension. Most
of the studies have been carried out with fish oils (eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA)). However, α-linolenic acid (ALA), found in green leafy
vegetables, flaxseed, rapeseed, and walnuts, desaturates and elongates in the body to
EPA and DHA and by itself may have beneficial effects in health and in the control of
chronic diseases.
Mushrooms are a good source of easily absorbed, high quality vegetable protein,
containing more than most other vegetables. Mushrooms are a good source of the B
vitamins - niacin, riboflavin, thiamin, folate, vitamin B6, biotin and pantothenic acid.
Mushrooms are a good source of the minerals potassium, selenium, copper and
phosphorus. Mushrooms contain more potassium than most other vegetables and
fruits. Mushrooms are also one of the few dietary sources of Vitamin D. Therefore,
this project aims at plant based sourcing for high value lipids, proteins and
carbohydrates with the following main objectives:
i. Screening higher plants for Omega 3/6 rich oils as healthy fats
ii. Analysing weed plant seeds for essential amino acid balanced proteins
iii. Mushroom sourcing and processing for protein and carbohydrates supplements
A total of 70 weeds have been collected so far under bioprospection activity and
are being analyzed in this project. Crude fat content has been determined for all the 70
weeds by a semi-continuous method using Soxhlet apparatus but total protein content
has been determined for 14 weeds by Bradford and Lowry method using UV-Visible
spectrophotometer
0
5
10
15
20
25
30
35
0-10 % 10 - 20 % 20-30 % 30-40 40-50
Nu
mb
er
of
Pla
nt
Sou
rce
s
Crude Fat Content
0
1
2
3
4
5
6
7
8
0-1 % 1-5 % 5-10 % 10-15%
Nu
mb
er
of
Pla
nt
Sou
rce
s
Total Protein Content
Initial leads for lipids and proteins (Quality aspect)
A. Lipids (Fatty acids)
S. No.
Fatty acid
Number of Plant sources
1 Eicosanoic acid 2
2 Linoleic acid 10
3 Linolenic acid 7
4 Oleic acid 10
5 Palmitic acid 9
6 Stearic acid 7
7 Arachidic acid 2
B. Protein (Amino acids)
S. No.
Amino acid
Number of Plant sources
1 Arginine 4
2 Histidine 2
3 Leucine 4
4 Isoleucine 3
5 Lysine 3
6 Methionine 3
7 Phenylalanine 4
8 Threonine 3
9 Cystine 1
10 Tyrosine 2
11 Valine 3
12 Asparagine 1
13 Ornithine 1
14 Canvanine 1
15 N-acetyl-L-djenkolic acid 1
4. Food matrix analysis and functional foods product development
Functional Foods which refer to processed foods containing ingredients that aid
specific bodily functions in addition to being nutritious offer great scope for
innovative products through R&D. Examples of functional foods include foods that
contain specific minerals, vitamins, fatty acids or dietary fibre, etc These can also be
the foods with added biologically active substances such as phytochemicals or other
antioxidants and even probiotics that have live beneficial cultures. The rise of
functional foods has occurred at the convergence of several critical factors, such as:
awareness of personal health deterioration, led by busy lifestyles with poor choices of
convenience foods and insufficient exercise; increased incidence and preferences of
self-medication; increased level of information from health authorities and media on
nutrition and the link between diet and health; scientific developments in nutrition
research; and a crowded and competitive food market, characterized by pressurized
margins. Food matrix is a carrier medium of nutrients/bioactive components/synbiotic
preparations and may also be a food in itself and therefore forms the core of any
functional food. With the aim to create dynamic platform of choices for food matrix,
the objective of this project aims for:
i. Screening and identification of new food matrices from millets and vegetable.
ii. Formulating functional foods by nutritional enrichment and balance.
iii. Process development and optimization for designer foods for common food
allergy suffering population
With respect to this, different nutritionally superior millets like jowar, bajra and
ragi in combination with cereals like rice and wheat and some horticultural biomass
categories are being explored to develop food matrices in the form of cookies, pasta
and noodles. The food matrices will be supplemented with bioactive components form
fruits and vegetable like Garcinia indica, Moringa oleifera, etc for its value addition
and further it will be explored as a carrier for probiotics. The prepared functional food
matrix will then be subjected to sensory, chemical and shelf life evaluation studies at
ambient temperature and conditions. The project also intends to develop and optimize
process for development of designer foods for the ailing population, i.e., gluten free
formulations for the gluten intolerant population, antioxidant and immunity boosting
functional foods for the immuno-compromised section of the society, etc. Initial leads
in such matrices and ingredient combinations are leading to value additions on cookie,
cracker, fruit bar and pasta formats as summarized below:
Category
Nutritional Value Addition
Cookie 1. Supplementation with a source of
bio-available vitamins and minerals which are the co-factors of
enzymes and co-enzymes
approximately 20 types of amino acids, 46 types of Anti-oxidants and
36 anti-inflammatory compounds
Vitamin A, B2, B3, B6, B9, B12, C, E, K, Calcium, Iron, Magnesium,
Phosphorus, Potassium, Sodium, Zinc
2. Providing a good source of calcium, manganese and iron.
3. Availability of two unique types of healing components: monoterpenes,
including carvone, limonene, and anethofuran; and flavonoids, including
kaempferol and vicenin.
4. Garlic advantage with Manganese, Vitamin B6, Vitamin C, Tryptophan,
Selenium, calcium, Phosphorus, Vitamin B1, Copper, Protein
5. Ginger flavour with contains Potassium, Magnesium, Copper, Manganese,
Vitamin B6
6. Selected seeds which are source of the minerals copper and manganese and
dietary fiber with monounsaturated fats have been included
7. Fruit sources for Vitamin A, Vitamin C, Thiamine, Riboflavin and Niacin have
been utilized
Cracker 1. Garlic advantage with Manganese, Vitamin B6, Vitamin C, Tryptophan,
Selenium, calcium, Phosphorus, Vitamin B1, Copper, Protein
Fruit Bar 1. Amaranthus as a very good source of vitamins including vitamin A, vitamin K,
vitamin B6, vitamin C, riboflavin, and folate, and dietary minerals including
calcium, iron, magnesium, phosphorus, potassium, zinc, copper, and
manganese
2. Plums as very good source of vitamin C, vitamin A, vitamin B2, potassium
and dietary fiber
3. Peach has potassium and vitamin C
Pasta 1. Millet included as good source of the minerals phosphorus, manganese and
magnesium
2. A refined cereal strips providing 66% fiber, 62% folate, 92% selenium and
99% phytochemicals that are essential for nutritional value
Projects planned for immediate future
1. Nutraceutical and bioactive phytochemicals from horticultural sources as
food ingredients and supplements
Beyond being the source of nutrients, foods, particularly derived from plant
sources, are a rich source of bioactive phytochemicals which are important for health
care. Research trends during the past 2-3 decades indicate that these phytochemicals
have an important role in preventing chronic diseases like cancer, diabetes, coronary
heart disease and hyper-cholesterolaemia. The major classes of phytochemicals with
disease-preventing functions are dietary fibre, antioxidants, detoxifying agents,
immunity-potentiating agents and neuropharmacological agents. Each class of these
functional agents consists of a wide range of chemicals with distinct biological
activities. For example, antioxidant function is exhibited by some nutrients, such as
vitamin E, vitamin C and provitamin A. Other phytochemicals that have antioxidant
properties are carotenoids, phenolic compounds, flavonoids and isothiocyanates. Some
of these phytochemicals show more than one functions. Foods rich in these chemicals
and exhibiting disease-protecting potential are called functional foods. Indian habitual
diets, which are based predominantly on plant foods like cereals, pulses, oils and
spices, are all good sources of these classes of phytochemicals, particularly dietary
fibre, vitamin E, carotenoids and phenolic compounds. Considering these points, this
project is designed with following objectives:
i. Analysis of horticultural crops, harvests and their wastes for antioxidants,
polyphenol and vitamin/micronutrient contents.
ii. Bioactive fractional component identification in fruits and vegetables to be
utilized as ingredients of foods for specific metabolic requirement.
iii. Process optimization and product development as food supplements and
functional foods
The project therefore intends to explore and analyze intelligently short-listed
horticultural crops like Bael, Garcinia, Moringa, Green tea, etc for their antioxidant,
polyphenol and vitamin/micronutrient content. The bioactive component from
different horticulture crops will be identified and subject to extraction and purification
for its utilization as ingredients of food supplements. The project also focuses on the
development and optimization of process for development of nutraceutical product.
With respect to this, till date different collections of Garcinia have been explored and
identified with respect to geographical locations. The main bioactive component in
Garcina, i.e. Hydroxy citric acid (HCA) primary useful for anti-obesity agent, has
been extracted and purified by different extraction method. A method for estimation of
HCA by TLC and colorimetric assay is under development and this will assist in
identification of Garcinia species with higher HCA concentration. Encapsulation of
bioactive component of fruit juices has been carried out with different mucilage in
spray dryer by optimizing different ratio of mucilage to juice concentrate.
2. Algal, fungal and microbial sourcing for enzymes, polysaccharides and
biomolecules
Recently, micro algae are emerging as a novel and renewable source of
biologically active compounds and nutritionally important macromolecules such as
Proteins, Carbohydrates, Lipids, Enzymes, Polysaccharides, Polyphenols,
Carotenoids, Vitamins and Minerals. However, the occurrence of bioactive
compounds in algal sources is less documented than that in higher plants. Therefore
micro-algae have a potential to explore the biologically active compounds which have
nutritional value and used as nutrient and micronutrients ultimately in the form of
functional food. As per the reported data Spirulina, Nostoc, Chlorella and Anabaena
have been exploited for nutritional and health values. Therefore it is needed to
explore the novel micro algae species from the natural sources which contain
biologically active compounds and useful nutritional macromolecules. Therefore the
project was taken up under these targeted objectives:
i. Screening of microalgae for nutritionally important proteins/enzymes and
carbohydrates
ii. Identification of new sources for oligosaccharides and lipids
iii. Biomolecules identification as nutraceuticals
Initially in a pilot study, 50 water samples were collected for Algae from water
bodies in Mumbai and Thane. Culturable algae found in only 6 water samples out of
random 15 water samples. These consisted of Chlorella colony, Oedogonium sps.,
Oedogonim like, Scenedesmus sps., Closterium sps., Microcystis sps. and Unidentified
sps. Simultaneous efforts were now on for survey and collection for lower plants with
focus on algae, mushroom and lichens towards nutraceutical value.
3. Food testing SOPs and safety standards diagnostics and QS
With rise of India as a resource hub of medicinal and nutraceutical plants and
establishment of FSSAI, it is not only desirable but mandatory requirement to validate
quality of functional foods and supplements in nutraceutical sector. The project
comprises of different analytical testing methods for the biochemical and thermal
analysis of different ingredients, food supplements and functional foods for quality
parameters from raw material and food products. The analytical methods involved
spectroscopic, chromatographic, titrimetric, gravimetric, and extraction techniques.
The analytical methods need optimization for extractions process, separation
techniques and quantification of food ingredients from crude materials. Also, the
determination of water quality parameters by the biochemical method is also
important for food manufacturing industries. The different SAPs and SOPs for the
analysis are under optimization/development. Project involves following objectives as
follow:
i. Development of analytical method for essential food ingredients such as;
Spectrophotometric method for protein, total carbohydrate,
hydroxycitric acid (HCA) analysis
Total fat by Soxhlet apparatus and fatty acid profile by GC
Sugar profile by HPLC
Polyphenols, carotenes, anthocyanins, and plant pigments etc. by
HPLC
Proximate analysis (ash, moisture, nitrogen etc.).
Water quality analysis for total dissolved solid, minerals, metals,
biochemical oxygen demand (BOD) and chemical oxygen demand
(COD)
Minerals and metals content by AAS/ICP
ii. Rapid diagnostics method for toxicity and adulteration analysis including
heavy metals and residues
iii. Microbial load and safety assurance analysis
Under these objectives, rapid spectrophotometric methods for total protein, total
carbohydrate, and hydroxycitric acid have been developed. The extraction process for
the protein, hydroxycitric acid, acidity, cholesterols, and nitrate and carotene from
solid/liquid food matrix is under optimization. The aim is to get NABL accreditation
of biological and chemical testing labs and be the analytical hub for nutraceutical food
testing as the recognized testing lab of Ministry of Food Processing.
Infrastructure
Research Centre
A. Research and Development
1. Plant Tissue Culture (Culture Handling)
2. Plant Tissue Culture (Growth Room)
3. Formulation Development (Ingredients)
4. Microbial Biotechnology (Fermentation and Culturing)
5. Media & Chemicals Handling (Sterilizers and Fumehood)
6. Molecular Biology (DNA, Protein, Lipid and Carbohydrate)
7. Fine Chemicals & Biologicals (Cryo- and Cold-storage)
8. Aseptic Room (Tissues and Cell Handling)
9. Herbarium (Plant collections)
10. Seed Repository (Crops and Weeds)
11. Crude Drug and Food materials repository
12. Bioinformatics (Database development and mining)
B. Analysis (Quality Testing)
1. Botany & Pharmacognosy (Analytical)
2. Bioprospection and Bioactivity Testing
3. Analytical Testing (Food and Chemical Testing Instrumentation)
4. Nutrition & Biochemistry (Analysis and method development)
5. Food Microbiology (Microbial Testing)
6. Food Analysis and Recipe Development
C. Processing (Bench scale - R&D)
1. Food Processing (Matrix and Products development)
2. Drying facility (Spray Dryer, Vacuum Dryer, etc.)
3. Baking and Steam processing
4. Oil expeller and extraction
5. Single screw extrusion for pasta and related products
6. Microencapsulation
7. Fruit processing (juices and pulps)
8. Cereal and pulse flours and dough making
Knowledge Centre
A. Learning through Resources
1. Library (Publications, Reports, Whitepapers, Brochure and Website/Digital Products)
2. Internet equipped computer systems
B. Facilitating through Technology
1. In-silico Research
2. Administration
C. Networking
1. Interaction zone
Specialized Facilities
In Operation
Analytical Instrumentation
Growth & Culture Rooms
Sterilization, Autoclaves & Quality Water
Chemical Handling Fume-hoods
Waste Recycling
Chemical Storage & Handling
Under Development
Cold Rooms & Conditioned Storage
Aseptic Clean Rooms/Labs
Controlled Condition Incubation
Biosafety Cabins
Dark Room & Photography
Distillation & Extraction
Incinerator & Decontamination
Linkages
1. Indian Agricultural Research Institute (IARI), New Delhi, India
NutraHelix Biotech Pvt Ltd. is a Corporate Member of Zonal Technology
Management and Business Planning and Development Unit, IARI and had also been
its business incubatee in the area of Nutraceuticals from October 1, 2010 to April 15,
2011.
2. Central Institute of Post Harvest Engineering & Technology (CIPHET),
Ludhiana, India
NutraHelix Biotech Pvt. Ltd. is registered with CIPHET for getting advisory
consultancy services and technologies on Post Harvest Agriculture and Processing of
Foods because CIPHET is a renowned institute and undertake lead researches in the
area of the post-harvest engineering and technology appropriate to agricultural
production catchment and agro-industries.
3. Indian Institute of Advanced Research (IIAR), Gandhinagar, India
NutraHelix Biotech Pvt Ltd. is collaborated with IIAR to conduct the applied and
analytical research in the frontier areas of science and to find application of the
research results for the benefit of mankind through nutraceutical products for
preventive healthcare. The two organizations are also willing and able to jointly carry
out technology development on the outcome research leads for industrial application
in food technology and products. This agreement describes those activities of interest
and priority areas that both signees have identified to focus in their collaboration, as
follows:
Special aim of the collaboration will be to identify novel plant sources for
Omega 3 and 6 fatty acid containing oil seeds to replace the fish oil need in
functional foods.
The second specific area identified is to develop various Aloe vera based safe
edible/skin care products ranging from juice/concentrates/gels/powders and
formulations.
The third area is of bioprospection to explore the new plant sources for
essential vitamins, amino acids and micronutrients and carry out research for
sustainable utilization of these resources.
4. Centre for Advanced Research & Training in Biosciences (CARTiB), Mumbai,
India
Both NutraHelix Biotech Pvt. Ltd. and CARTiBs have recognized their
complementary partnership feasible because of the common interest to carry out
research, offer skill development training and take up technology development in the
areas of "Bio-Science" and "Natural Products" leading to their use in the fields of
biotechnology, pharmaceuticals, health & nutrition, industrial applications, agriculture
and environment. Both organizations express their willingness to carry out
collaborative research in specific areas that will be identified time to time through
mutual discussion and consent on time bound projects mode.
5. Joint Genomic Center (JGC), Sofia, Bulgaria
NutraHelix Biotech Pvt Ltd and JGC have agreed to work together to advance the
scientific research through approaches intended to foster and stimulate additional
scientific exchange and collaboration between individual scientists and research
institutions in both countries. Jointly, they will identify broad areas of mutual interest,
and general activities that could be carried out in support of their shared priorities and
objectives. This agreement establishes the intent between NutraHelix Biotech Pvt Ltd
and JGC to work in a collaborative relationship for research and business activities of
interest and priority that both signees wish to focus are the following:
Priority to be given to the technology development through research related to
identification, characterization, studying of the properties, food ingredients and
health properties in traditional Bulgarian and Indian products, which play a
major role in maintaining human health.
Evolve technologies for world class safe and efficacious products of nutrition
using food/medicinal/cosmetic routes after thorough scientific validation
utilizing modern tools of molecular biology and biotechnology for biological
activity and toxicity/safety data generation.
Development of processes and products in functional foods and nutraceuticals
sectors the target of R&D and/or technology with business development.
Exchange of human resource skills, expertise, research materials and
information on specific topics of mutual interest and Identifying each other as
collaborators through their websites and print materials
Jointly prepare and submit technology based project schemes for international
support and commercialization routes
Publications
Monographs in Preparation
Aegle marmelos (Bael)
Aloe vera related species (Ghrita-kumari)
Amaranthus species (Ramdana)
Avena sativa L (Oats)
Bacopa monnieri (Brahmi)
Boerhavia diffusa (Punarnava)
Centella asiatica (Mandukparni)
Curcuma longa (Turmeric)
Euryale ferox (Makhana)
Fagopyrum esculentum (Buck wheat)
Garcinia species (Kokum)
Grewia species (Phalsa)
Juglans regia (Wallnut/Akhrot)
Linum usitatissimum L. (Flax)
Moringa oleifera (Drum Stick/Sehjan)
Ocimum species (Tulsi/Basil)
Phyllanthus emblica (Aonla)
Phyllanthus species (Bhui-amalki)
Psidium guajava (Guava)
Punica granatum (Pomegranate/Anaar)
Rhododendron species (Rose tree)
Stevia rebaudiana (Stevia)
Tamarindus indica (Imli)
Zingiber officinale (Ginger)
Ziziphus jujube (Ber)
Research and Reference Publications
Khanuja SPS, Jhang T, Shasany AK (2010) Medicinal and aromatic plants: A
case example of evolving secondary metabolome and biochemical pathway
diversity. In: Nature at work: Ongoing saga of evolution, (Editor: V.P. Sharma)
1st Edition, National Academy of Sciences, India, Springer (India) Private
Limited, New Delhi 110002, pp 355-368
Suman P.S. Khanuja (2010) Review of the book: Plants Affecting Human
Mind (Psycho Active Plants) by SK Jain. In: Proc Indian Natn Sci Acad 76 (1):
53-54
Khanuja SPS, Shukla AK (2011) Human health and nutrition: Functional
foods. In: Horticulture to Horti-Business (Editors: KL Chadha, AK Singh, VB
Patel), Proceedings Book of the Fourth Indian Horticulture Congress held at
New Delhi during 18-21 November, 2010, Westville Publishing House, New
Delhi, pp 433-445
Expert Services
Research Process Outsourcing (Food & Nutrition)
Novel Functional Foods and Nutraceutical Products (B2B)
Project Mentoring: Agri-food Biotech Innovations & Processes
Product Testing/Quality Validation & Bioassays Services
KPO (Literature / Feasibility/Projects/Information)
Concept to Co-development of products with Nutra Industry
Participation in National and International Events and Workshops
Training on “Microbiological and Biochemical Techniques involving
Isolation, Purification and Characterization of Microorganisms” on October
18-November 17, 2010 at Indian Agricultural Research Institute (IARI), New
Delhi
HPCL Congress India on December 16-17, 2010 at Grand Hayatt Hotel,
Mumbai
Training on “Molecular Biology and Biochemical Techniques involving DNA
Methods, PCR Tools and Molecular Markers and Diagnostic Probing of
microorganisms and higher plants” on January 3 – February 2, 2011 at Indian
Agricultural Research Institute (IARI), New Delhi
6th
Nutra India Summit 2011 on February 16-18, 2011 at World trade centre,
Mumbai
Session on "Ayurceuticals" The Marriage of Traditional Knowledge and
Modern Science on February 15-18, 2011 at World Trade Centre, Mumbai
Committed Participations:
Pharma-Nutrition conference on April 18-20, 2011 at Amsterdam, The
Netherlands
Bangalore INDIA BIO on May 4-6, 2011 at Bangalore International Exhibition
Centre, Bangalore
ICAR-CII Industry Meet on May 23, 2011 at NASC Complex, New Delhi
3rd
International Conference on "Nutraceuticals, Functional Foods and Dietary
Supplements" on June 23, 2011 at Intercontinental – The Lalit, Mumbai
National Conference on Career and Research Trends in Food Processing on
June 24-25, 2011 at Indian Institute of Crop Processing Technology (IICPT),
Thanjavur, Tamil Nadu
7th
Food & Technology Expo – 2011 on July 29-31, 2011 at Pragati Maidan,
New Delhi
Lead Lectures and Keynote Addresses by Chief Mentor
1. Phytochemicals for Human Health Care - Lead Lecture by Dr SPS Khanuja in the
session "Role of Phytochemicals and Nutraceuticals in human nutrition and health" of
the International Conference on Role of Biomolecules in Food Security and Health
Improvement, on February 17-20, 2010 at BHU, Varanasi
2. Aromaceuticals - the way forward for opportunities in essential oils and aromatics -
Keynote Address by Dr SPS Khanuja in the first Technical Session of Asian Aroma
Ingredients Congress & Expo 2010 on May 6-9, 2010 at Hotel Leela, Gurgaon
3. Phytonutraceuticals: the molecules for health from plant metabolism -Public Lecture
delivered by Dr SPS Khanuja at Centre for Converging Technologies (CCT) for
students and faculty on August 6, 2010 at University of Rajasthan, Jaipur
4. Genes to genomes: the discovery path of biotechnology" – Academic Lecture
delivered by Dr SPS Khanuja for students and faculty of JNU on August 7, 2010 at
Jaipur National University, Jaipur
5. Plant metabolome based strategic research need for designing and breeding
nutraceutical crops – Theme Lecture by Dr SPS Khanuja in NAAS Brainstorming
Session on “Prioritization and value addition of nutritionally important crops” on
September 18, 2010 at National Academy of Agricultural Sciences, National
Agricultureal science Complex, New Delhi
6. 4th
Indian Horticulture Congress 2010-Invited Lecture delivered by Dr SPS Khanuja
Organized by NSFI (National Skill Foundation of India) and HIS (Horticultural
Society of India) on November 18-21, 2010 at National Physical Laboratory, New
Delhi
7. Revisiting horticulture as health sector catalyst through fruit genomes and metabolites
- Lecture by Dr. SPS Khanuja. in the 6th
Nutra India Summit 2011 on February 16-18,
2011 at World Trade Centre, Mumbai
Launching of NutraHelix Research Centre at 6th Nutra India Summit 2011, World Trade Centre, Mumbai on 16th February 2011
Statement of Assets and Costs
Certificate for cost of Fixed Assets owned by the company
Certificate for Fixed Assets taken on lease by the company
Certificate for Preoperative Expenses and Other Advances and
deposits
SANJAY SAMDANI & ASSOCIATESChartered Accountants
r-- ~- --
iCA"""\, Ii / II . j! I\ J
v, --_ ..... /
106-D, Trade Corner,Sakinaka, Mumbai 400 072
2: 022-28526152, Tele-fax:022-28520621Email: [email protected]
ToNutrahelix Biotech Pvt. Ltd.19-A, Sita Estate,AzizBaug, Mahul Road,Chembur,Mumbai 400074
Date: 03/08/2011
Sub : Certificates
Please fmd attached herewith following three certificates as requested by you forapplication and registration purpose.
1. SSA/ 11-12/Nutra 02 Certificate for Cost of Fixed Assets owned by the company2. SSAj11-12 / Nutra 03 Certificate for Fixed Assets take on lease by the company3. SSA/ 11-12/Nutra 04 Certificate for Preoperative Expenses and Other Advances
and deposits
We hope you will find the above in order.
~~.SANJAYSAMDANIProprietor
SANJAY SAMDANI & ASSOCIATESChartered Accountants
------------\(CA,lI _ i
I :\ /\\~- ---~
106-D,Trade Corner,Sakinaka, Mumbai 400 072
if: 022-28526152, Tele-fax:022-28520621Email: [email protected]
Cert. No,:- SSA/ 11-12/Nutra 02
CERTIFICATE
This is to certify that we have verified the books of accounts and other relevant
records and documents of NUTRAHELIX BIOTECH PVT. LTD having its registered office
as at 19A, Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074 and based
on such verification and representation, we certify below the cost of Fixed Assets owned by the
Company as on 31.03.2011:
S.No, Fixed Assets Amount (Rs.l
1.
2.
3,
Lab Equipments
Computers
Office Equipments
1,76,390,00
2,00,851.00
11,243.00
Total 3,88,484.00
This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.
For SANJAYSAMOANI& ASSOCIATESChartered AccountantsFirm Registration No. 109183W
~,
SANJAYSAMOANIProprietorM.No.: 041057Place: MumbaiDate: 05. tJg'U'1/
SANJAY SAMDANI & ASSOCIATESChartered Accountants
(CA··-----\i . ~,i [\ j
\,.._--j
106-D, Trade Corner,Sakinaka, Mumbai 400 072
if: 022-28526152, Te1e-fax:022-28520621Email: [email protected]
Cert. No.:- SSAI 11-12/Nutra 03
CERTIFICATE
This is to certify that we have verified the books of accounts and other relevant records and
documents of NUTRAHELIX BIOTECH PVT. LTD having its registered office as at 19A,
Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074, and based on such
verification and representation, we certify the statement of Cost of Fixed Assets taken on lease
from Mis. Nandan Impex Private Ltd.as on 31.03.2011,which are as under:
S.No. Fixed Assets Amount (Rs.)
l. Lab Equipments 22,18,854.00
2. Computers 1,51,816.00
3. Office Equipments 68,33,720.00
4. Factory Building 38,99,42l.00
------------------Total 1,31,03,81l.00
=============This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.
For SANJAYSAMOANI& ASSOCIATESChartered AccountantsFirm Registration No. 109183W
~~~
SANJAYSAMOANIProprietorM.No.: 041057Place: MumbaiDate : 03· 0 g .')-c I I
SANJAY SAMDANI & ASSOCIATESChartered Accountants
(rl\--"\Iv~(!\ ./'-".. -
106-D,Trade Corner,Sakinaka, Mumbai 400 072
if: 022-28526152, Tele-fax:022-28520621Email: [email protected]
Cert. No.:- SSA/ 11-12/Nutra 04CERTIFICATE
This is to certify that we have verified the books of accounts and other relevant records anddocuments of NUTRAHELIX BIOTECH PVT. LTD having its registered office as at 19A,Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074 and based on suchverification and representation, we certify Pre-operative Expenses incurred and OtherAdvances & Deposits given by the Company as on 31.03.2011as under:
A)PR-EOPERA11VE EXPENSES:
S,No.1.2.3.4.5.6.7.8.9.10.11.12.13.14.
S.No.1.2.3.4.5.
.ParticularsSalary & Allowance to StaffStaff Welfare ExpensesConveyance & Travelling ExpensesVehicle ExpensesElectricity ExpensesConference ExpensesFees & Subscription ExpensesPostage & Telegram ExpensesOther Office & Admin. ExpensesBank ChargesPreliminary ExpensesLease RentLaboratory ExpensesRepair & Maintenance
BlaTHER ADVANCES& DEPOSITS
ParticularsAdvances against MachineryMVATCredit availableAdvances against expensesDeposit of VATStaff Advances
Total
Arnount(R3.}6,85,53G.tJO
28,089.0040,686.0077,985.0014,520.00
2,00,117.0058,876.2059,214.00
3,06,777.002,097.50
99,300.008,00,000.004,03,752.85
30,642.00
Amount(Rs.l4,27,500.00
69,643.0038,408.0025,000.004,000.00
Amc'untf.Rs.}
28,07,643.00
5,64,551.0033.72,194.00
This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.
SANJAYSAMDANIProprietorM.No.: 041057Place: Mumbai