REPORT: 180993996

ANALYSIS RESULTS FEED - TOXINS - EXTENSIVE MONITOR

801.QRVC

remarks:

A S P E R G I L L U S

Aspergillus fumigatus var. fum. 1204 60200 60200

Aspergillus f. var. fum. Azol-resistant < 1 0 45

Other aspergillus fumigatus sc. 2496 124800 124800

Aspergillus terreus < 1 0 45

Aspergillus section nigri < 1 0 45

Aspergillus parasiticus < 1 0 45

Aspergillus spp. < 1 0 45

C L O S T R I D I A

Clostridium botulinum < 17 0 16

Clostridium perfringens < 17 0 16

2400 2400 2400

Other sulfite reducing clostr. 55 55 55

V A R I O U S F U N G I

Penicillium roquefortine group 250 250 250

Penicillium griseofulvum < 150 0 140

Penicillium spp. < 150 0 140

Monascus ruber < 150 0 140

Geotrichum candidum 37750 37750 37750

Fusarium spp. < 150 0 140

Other fungi 75925 75925 75925

Yeast and other fungi 16325 16325 16325

C A N D I D A

Candida albicans < 50 0 45

Candida krusei 5000 5000 5000

Candida glabrata < 50 0 45

Candida tropicalis < 50 0 45

Legend

Reportnumber: 180993996 4-Jun-2019Layoutnr.: 10-2017 8QRVC.XLTX

sample

analysis

interpretation more info:

VOORBEELD ANALYSE

Bodemroute 47

0123 NN BODEMDAM

Sample name Sample nr. Code analysis

Feed 93996

*)

*)

Clostridia spp.

increased / unfavorable

too high / great chance of issues caused by toxins and digestion problems digestion problems

extremely high, often the direct or indirect cause of health issues

*) Not detected

target value / good / acceptable

K o c h - E u r o l a b

L a b o r a t o r i u m c h e m i s c h e n m i c r o b i o l o g i s c h - A g r a r i s c h e k r i n g l o o p a n a l y s e sP r o d u c t t e c h n o l o g i e - D u u r z a a m h e i d m i l i e u a l g e m e e n

Postbus 21 7400 AA DEVENTER (NL) Tel. 0570 50 20 10 Fax 0570 652279 KvK. 38022558 E-mail info@eurolab.nl www.eurolab.nl BTW/VAT/ID nr.: nl 8032.19.398.B.01

INTERPRETATION ANALYSIS RESULTS FEED - TOXINS - EXTENSIVE MONITOR

Koch - Eurolab 4-Jun-2019

Layout: 08-2017 8QRVC.XLT Report: 180993996

Aspergillus spp. (selective microbiological analysis, microscopically confirmed by method KOCH) Results are shown in

ASP units. Other Aspergillus species that have not been mentioned above.

The quality of the forage consists not only of minerals and nutritional value, but also from the presence or absence of

harmful bacteria, yeasts and fungi, toxins and (natural) antimicrobials. Even though a good rumen digestion does break

some harmful substances, it is possible that the forage already contains toxins or natural antibiotic substances that are

absorbed by the animal. In practice we see that livestock fed with contaminated forage in about 65% of cases also contain

harmful fungi in the intestines, which can lead to intestinal inflammation (Source: Koch - Eurolab, research in

collaboration with Veterinary Medicine, Faculty of Utrecht). The detection of these harmful influences in feed and the

intestines makes it possible to resolve previously almost insoluble and unclear animal health problems by taking the

appropriate measures. In practice, these measures appear to lead more often to a reduction of costs rather than

additional costs, which makes these toxins analyzes suitable for routine purposes. In this way, any latent, subclinical

problems can be addressed in time and worse can be prevented. It is beneficial to perform this checkup 3-4 times a year

(up-to-date feed mix and faeces) in order to keep the cycle at your company clean in the future and to keep the ration

optimal.

General advice: The following measures can be taken if there was a contamination detected in the animal feed:

(1) Make sure there is a good feeding rate, i.e. for example an open pit roughage is broached very frequently. In case of

structural problems baled silage can be a solution.

(2) Ensure that by-products are sufficiently fresh and stored in a well-preserved manner.

(3) Have the feed analyzed that might be contaminated.

Aspergillus fumigatus sc. and terreus (selectieve microbiologische analyse, microscopically confirmed by method KOCH)

Results shown in ASP units. Both fungi secrete the immune system damaging gliotoxin and also several other mycotoxins.

As a result, the resistance to diseases of the animal is reduced, so that in the long run numerous problems can arise in the

cattle. These two Aspergillus species are often found in feed (grass and maize), byproducts and grains, in less quantities.

The contamination of the feed often turns into a total digestion infection. As a result, toxins are continuously produced

within the animal, in addition to those that are possibly already present in the feed. The internal contamination can

develop into an intestinal inflammation, in which the feed intake is disturbed. This means that the animal gets less from

the feed and also gives less milk than what can be expected according to the diet and the normal performance level. Over

time, this leads to more serious bowel inflammation, which can show symptoms of wear and tear. If there is a high

amount detected in the feed mixture, it is useful to check which feed material is responsible for this and to keep it outside

the diet. A faeces analysis is also necessary to check whether the contamination has continued within the digestion

system. By following the specific advice, the contamination can then be fought before further deteriorating of the

condition of the animals.

Aspergillus section nigri (selective microbiological analysis, microscopically confirmed by method KOCH) Results are

shown in ASP units. Less often occurred harmful fungal species.

Aspergillus parasiticus (selective microbiological analysis, microscopically confirmed by method KOCH) Results are

shown in ASP units. Less often occurred harmful fungal species. The Aspergillus parasiticus can produce the aflatoxin that

can be transferred to milk.

K o c h - E u r o l a b

L a b o r a t o r i u m c h e m i s c h e n m i c r o b i o l o g i s c h - A g r a r i s c h e k r i n g l o o p a n a l y s e sP r o d u c t t e c h n o l o g i e - D u u r z a a m h e i d m i l i e u a l g e m e e n

Postbus 21 7400 AA DEVENTER (NL) Tel. 0570 50 20 10 Fax 0570 652279 KvK. 38022558 E-mail info@eurolab.nl www.eurolab.nl BTW/VAT/ID nr.: nl 8032.19.398.B.01

INTERPRETATION ANALYSIS RESULTS FEED - TOXINS - EXTENSIVE MONITOR

Koch - Eurolab 4-Jun-2019

Layout: 08-2017 8QRVC.XLT Report: 180993996

Other fungi (microbiological analysis) It is not always possible to determine which species they are detected and whether

they are harmful. This is only an indication of the presence or absence of other fungi. It is possible to identify these within

a few days after the result, if ordered. The costs of (RNA) identification are € 240, - per species.

Yeasts (microbiological analysis) These are not harmful organisms by themselves, this is often the same type of yeast that

is used in beer. However, these are less desirable in pits because they increase the pH of the feed and thus make it

possible for fungi to grow in the silage, on the cutting edge of the silage and between silage and feeding.

Candida (microbiological analysis) Candida albicans, Candida krusei, Candida glabrata and Candida tropicalis are harmful

yeasts. They have been more frequently been detected in feed since a couple of years (result shown in k.v.e./g).

Clostridium botulinum (selective microbiological analysis, microscopically unconfirmed) This bacteria is better known as

Botulism. If desired, further research can be carried out by malditov € 90,- . An infection in the gut system can be

eliminated by adaption measures to the diet (result shown in k.v.e./g).

Clostridium perfringens conform NEN-EN-ISO 7937 The analysis detected suspicious Clostridia perfringens, which is yet to

be confirmed by malditov € 90,- (as additional optional research). This bacteria produces, for example, the nerve poison

lecithinase, an enzyme that affects the nerves. An infection in the gut system can be eliminated by adaption measures to

the diet (result shown in k.v.e./g).

Clostridium spp. conform NEN-EN-ISO 7937 (microbiological analysis) These are various Clostridia, no botulinum or

perfringens. Within this broad group of Clostridia not all species secrete toxins. If desired, further identification can be

determined.

Other sulfite reducing bacteria conform NEN ISO 15213:2003 (microbiological analysis) Clostridia botulinum, perfringens

and the Clostridium spp. are (also) sulphite reducing bacteria. Under the title of other sulphite reducing bacteria are only

the non-Clostridia shown. Sulphite reducing bacteria are an extensive group of bacteria which have the similarity that they

produce sulphides, which can fix trace elements such as zinc, manganese, cobalt and copper during the digestion process.

The origins of these bacteria, a low oxygen soil among other causes, is comparable to other Clostridia in the analysis list,

which also produce sulphides.

Heat forming bacteria (microbiological selective analysis, microscopically confirmed, method Koch) These are bacteria

that can accelerate the heating. If heat is forming by rapid speed, a high feed rate of the pit is desired, or a silage wall

treatment (optional determination).

Penicillium roquefortine group (selective microbiological analysis, microscopically confirmed, method Koch) Infections

caused by the Penicillinium roquefortine group are very common. These do not produce any truly toxic mycotoxins, but

they do cause a less optimal digestion. This can lead to other infections. It is common in this case to find a (slightly lower)

contamination in the feed in comparison to faeces (if a sample was provided).

K o c h - E u r o l a b

L a b o r a t o r i u m c h e m i s c h e n m i c r o b i o l o g i s c h - A g r a r i s c h e k r i n g l o o p a n a l y s e sP r o d u c t t e c h n o l o g i e - D u u r z a a m h e i d m i l i e u a l g e m e e n

Postbus 21 7400 AA DEVENTER (NL) Tel. 0570 50 20 10 Fax 0570 652279 KvK. 38022558 E-mail info@eurolab.nl www.eurolab.nl BTW/VAT/ID nr.: nl 8032.19.398.B.01