Anaerobic digestion of algae biomass: A review
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ble substrate, recalcitrant substrate constituents, cell wall degradability, low carbon
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4. Problems with anaerobic digestion of microalgae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0
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Algal Research xxx (2014) xxxxxx
ALGAL-00107; No of Pages 11
Contents lists available at ScienceDirect
j ourna l homepage: www.e lsev ie r .com/ locate /a lga l6.3. Sulfur and its role in anaerobic digestion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 07. Bacterial consortium and its role in anaerobic digestion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 06. Inhibition of anaerobic digestion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6.1. Ammonia-nitrogen toxicity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6.2. Saline microalgae and the effect of salinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4.1. Low concentration of digestible substrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 04.2. Cell wall degradability and pre-treatment of microalgae biomass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 04.3. The carbon/nitrogen ratio associated with microalgae biomass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 04.4. Lipids and microalgae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0
5. Theoretical methane production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 08. Anaerobic digestion and nutrient recycling
Corresponding author at: The University of Adelaide410389528 (mobile); fax: +61 8 83134373.
E-mail address: Andrew.firstname.lastname@example.org (A.J. W
http://dx.doi.org/10.1016/j.algal.2014.02.0012211-9264/ 2014 Elsevier B.V. All rights reserved.
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3. Microalgae and anaerobic digestion . . .
3.1. Historical and current perspectivesContents
1. Introduction . . . . . . . . . .1.1. Algae based biofuels . . .1.2. Historical perspective of ana
2. Macroalgae and anaerobic digestionCurrent productionmethods for liquid biofuel production frommicroalgae produce approximately 6070% resid-ual biomass that is currently a byproduct. Anaerobic digestion provides biogas, but it can also provide essentialnutrient recovery from lipid extracted microalgae biomass. The biogas produced from the anaerobic digestionprocess can be used to generate onsite electrical power or thermal heat to offset biomass processing and extrac-tion processes. When both of these processes are integrated and operated simultaneously, the benets tomicroalgae biofuel production and wastewater treatment derived energy production are increased signicantly.To consider the integration of anaerobic digestion into a commercial-scale integratedmicroalgae production andbiofuel renery facility or wastewater treatment plant we present a review of the literature, the current state ofthe art and future directions for research.
2014 Elsevier B.V. All rights reserved.
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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0igestion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0Anaerobic digestionNutrient recovery
to nitrogen ratio, ammonia toxicity and effects from salinity and associated metal ions.
Microalgae tion of digestible biodegrada
presents an opportunity forgestion ofmicroalgae biomasReview article
Anaerobic digestion of algae biomass: A review
A.J. Ward a,, D.M. Lewis a, F.B. Green b
a Microalgae Engineering Research Group, The University of Adelaide, School of Chemical Engineering, Adelaide, South Australia, Australiab Oswald Green Technologies, Inc., USA
a b s t r a c ta r t i c l e i n f o
Article history:Received 22 July 2013Received in revised form 29 January 2014Accepted 1 February 2014Available online xxxx
The anaerobic digestion of microalgae is a prospective environmentally feasible option for creating a renewablesource of energy for industrial and domestic needs. Microalgae anaerobic digestion is a key unit process that in-tegrates efciency and benecially into the production ofmicroalgae derived biofuels. Anaerobic digestion culmi-nating in methane fermentation improves the economic viability of microalgae liquid biofuel production and
power generation from wastewater derived microalgae. However the anaerobic di-s is not straight forward due to several technical restraints including low concentra-. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0
, School of Chemical Engineering Gate 5, Frome Road, Adelaide, South Australia 5005, Australia. Tel.: +61 8 8313 3150, +61
Anaerobic digestion of algae biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
9. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0
1.1. Algae based biofuels
With the current increasing global population and the associated in-crease in fossil fuel use and demand, there has been an increased inter-est in renewable energy sources based on biomass transformation. The use of agricultural derived biomass to produce biofuels hasgained momentum [4,5]. This push for agricultural based biofuel pro-duction can lead to other less obvious problems for example eutrophica-tion, resource depletion, reduced biodiversity due to current farming
illustrates the anaerobic digestion of biomass after cell wall disruptionprior to conversion. The third pathway is the traditional biodiesel prac-tice where lipid is extracted and residual algal biomass is converted tobiogas by anaerobic digestion and methane fermentation.
1.2. Historical perspective of anaerobic digestion
Historically anaerobic digestion has been exploited for thestabilisation of raw, domestic sewage sludge that is typically removedfrom primary sedimentation basins . However anaerobic digestionfor bio-methane production has received renewed attention due to its
2 A.J. Ward et al. / Algal Research xxx (2014) xxxxxxAnaerobic Digestion
MethaneNutrients recyclepractices and the direct competition with current food crops [5,6].Microalgae offer an interesting alternative feedstock for the produc-
tion of biofuels. Microalgae have a high total yield and hence a lowerland use footprint and can utilise land areas that are unsuitable forfood production [1,7]. In additionmicroalgae production has the poten-tial to utilise CO2 emissions and offers the potential for a carbon neutralbiofuel .
Biofuel production frommicroalgal feedstock has several challengesto overcome before it can become a mainstream industry capableof producing the quantity of biofuel required at a competitive price.Challenges faced by the industry include demand for fertiliser due tomicroalgae's signicant utilisation of nutrients, high energy inputsrequired for harvesting and dewatering biomass and for the lipid ex-traction and conversion processes. Anaerobic digestion can offer a path-way to eliminate some of the overheads of the production cycle byproducing biogas for utilisation in electricity production or thermal en-ergy production. This benet is highly dependent on the biofuel plantprocess and location, and the costs associated with natural gas pricesand electricity prices will determine the efciency improvementresulting from the biogas utilisation. In addition, the recovery of valu-able nutrients from biomass via anaerobic digestion is essential for thesustainability of the algae biofuel industry. It is anticipated that the in-corporation of anaerobic digestion in microalgae biofuel productionand bio-renery processes will increase the cost effectiveness of theproductionmethods, helping it to becomeeconomically feasible and en-vironmentally sustainable. Fig. 1 illustrates the conceptual implementa-tion of anaerobic digestion into algal production processes. Threepathways have been dened: pathway 1 shows the direct anaerobic di-gestion after the biomass harvest and concentration step. Pathway 1could be utilised in a wastewater process where the cell wall is degrad-able by bacterial activity within the digester. The second pathway
1. Direct Conversion
Concentration DisrupFig. 1. Conceptual visualisation of anaerobic digesti
Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001viability as an alternative and renewable fuel source [10,11].Municipal and Industrial anaerobic digestion of organic waste
streams is widely practiced and recognised as a mature technology forproducing biogas . Anaerobic digestion has been included in therst generation of biofuels that have been developed in recent years.The rst generation biofuels have focused largely on the productionof biofuel from terrestrial plant crops . In these systems solar energyis used to drive the photosynthetic xation of carbon dioxide to organicmatter. The energy crop is harvested and then used directly as a com-bustible fuel or converted to another form such as ethanol, hydrogenor methane . These rst generation biofuels have been highlycriticised for their use of valuable food crops as feedstocks for fuel pro-duction. This criticism is due to the utilisation of valuable agriculturalland and scarce water resources for feedstock production. With the up-ward pressure exerted on food prices, biofuel production from foodcrops has been deemed unsustainable [6,16].
2. Macroalgae and anaerobic digestion
Interest in the cultivation of microalgae for the production of bio en-ergy, as one form of solar energy, was born in the 1950s, but when theglobal supply of oil was interrupted twice during the 1970s, interest inthe cultivation of highly productive macroalgae for bio energy produc-tion was accelerated . Macroalgae received a large amount of atten-tion as a biofuel feedstock due to its prolic growth in eutrophic coastalwater fouling beaches and coastal waterways. Anaerobic digestion hasbeen used to dispose and process thismaterial for the production of bio-gas [11,17]. Development of feedstocks for methane biogas productionfrom macroalgae biomass led to signicant plans for extensive marinefarms [18,19], but these failed as the economic and geopolitical climatebecame favourable once again for fossil fuels.
. Disruptediomass 3. Lipid Extracted
Biofuelon incorporation into algal biofuel production.
e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
Some of the problems that have been associated with anaerobicdigestion of marine macroalgae include recalcitrant material such aspolyphenols, cellulosic bres and lignin type components resulting inthe reduced biodegradability of the biomass by bacterial processes,hence limiting digestibility and gas production [20,11]. Also some highsulde containing macroalgae species have been found to inhibit theanaerobic digestion process . Other problems associated with theuse of macroalgae for biofuel production include the seasonal growthassociated with different types of macroalgae and hence variable feed-stock for biogas production [11,17]. Macroalgae are again receivingattention as a substrate for anaerobic digestion, but macroalage arenot discussed in the context of this review article.
3. Microalgae and anaerobic digestion
3.1. Historical and current perspectives
The focus on biofuel production is shifting towards what are knownas second and third generation biofuels . The second generation ofbiofuels utilise alternatives to food sourced biomass crops for feedstocksin biofuel production. The third generation or advanced biofuels aresourced from non-food crops, but the resulting fuel is indistinguishablefrom its petroleum counterparts . A promising approach within thesecond and third generations of biofuels is the use of microalgae as abiofuel feedstock . Microalgae are highly productive and are ableto produce large quantities of biomass more efciently than current
cultivation practices for terrestrial crops. The photosynthetic efciencyof microalgae in engineered systems can reach 45% of the solar energycompared to 12% for terrestrial plants .
The rst authors to report on the anaerobic digestion of microalgaebiomasswere Golueke et al. . They investigated the anaerobic diges-tion of Chlorella vulgaris and Scenedesmus, microalgae species grown aspart of a wastewater treatment process. Oswald, the co-author from theGolueke et al. [22,23] papers, continued research on anaerobic digestionof microalgae that is reported in a series of scientic publications de-scribing the role ofmicroalgae in sewage treatment using Advanced In-tegrated Wastewater Pond Systems .
This early work by Oswald and co-authors identied several key fac-tors that could hinder the digestion ofmicroalgae biomass; these factorsare discussed later in this review article. The data shown in Table 1 pro-vides a summary of the research that has been undertaken on severaldifferent microalgae species to date and highlights the gas potentialfrom microalgae as a viable process for the production of biogas.
The lowest gas production recorded from freshwater microalgaebiomass was ~70 mL g1 VS (volatile solids) for untreated Microcystissp. . The gas production reported in this experiment was low dueto the researcher's investigating inoculum start up volumes during thebio-methane potential assays rather than maximising gas productivity.The authors later recorded a gas production of 153 mL g1 VS for thesame microalgae species utilising an optimised inoculum ratio. The au-thors Lakaniemi et al.  reported a low production rate of 24 mL g1
VS for the saline microalgae species Dunaliella tertiolecta. This low
Table 1Methane production from the anaerobic digestion of microalgae biomass reported in scientic literature. (NR = Not reported).
Microalgae species C/N Ratio Methane yield Loading rate Reference
Arthrospira maxima 4.35.33 173 mL g1 VS 500 mg/TS/L Arthrospira platensis N/R 481 mL g1 VS 2000 mg/TS/L Blue green algae N/R 366 mL g1 VS 281.96 mg/VS/L Chlamydomonas reinhardtii N/R 587 mL g1 VS 2000 mg/TS/L Chlorella kessleri N/R 335 mL g1 VS 2000 mg/TS/L Chlorella sp., Pseudokirchneriella sp. and Chlaqmydomas sp. N/R 0.280.60 m3/kg/VS 402 mg VS Chlorella sp., Scenedesmus, Euglena and Oscillatoria N/R 300800 mL g1 VS N/R Chlorella sp., Scenedesmus N/R 170320 mL g1 VS 1.442.89 g/VS/L Chlorella sorokiniana N/R 212 mL g1 VS N/A 
3A.J. Ward et al. / Algal Research xxx (2014) xxxxxxChlorella vulgaris N/RChlorella vulgaris N/RChlorella vulgaris 6Chlorella vulgaris N/RChlorella vulgaris N/RDunaliella N/RDunaliella salina N/RDunaliella tertiolecta N/RDurvillea Antarctica N/REuglena gracilis N/RLake Chaohu natural population consortium N/RMacroystis pyrifera and Durvillea Antartica(50% blend) N/RMacroystis pyrifera N/RMicrocystis sp. N/RNannochloropsis oculata N/RNannochloropsis salina (lipid extracted biomass) 4.4Phaeodactylum tricornutun N/RScenedesmus obliquus N/RScenedesmus obliquus N/RScenedesmus sp. N/RScenedesmus sp. (single stage) N/RScenedesmus sp. (two stage) Note: 46 mL/g/VS Hydrogen N/RScenedesmus sp. and Chlorella sp. N/RScenedesmus sp. and Chlorella sp. 6.7Spirulina Leb 18 N/RSpirulina maxima 4.16Spirulina maxima N/RSpirulina maxima N/RSpirulina platensis UTEX1926 N/RTetraselmis 7.82C/N ratio-Waste water grown community N/RZygogonium sp. N/RPlease cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001403 mL g VS 2 g/VS/L 286 mL g1 VS 5000 mg/VS/L 240 mL g1 VS 1000 mg/VS/L 189 mL g1 VS N/R 0.400.45 L 26776714 mg (COD) 440 mL g1 VS 910 mg/VS/L 505 mL g1 TS 2000 mg/TS/L 24 mL g1 VS 5000 mg/VS/L 492 mL g1 VS 3000 mg/dry/TS/d 485 mL g1 VS 2000 mg/TS/L 295 mL g1 VS N/R 540 mL g1 VS 3000 mg/dry/TS/d 545 mL g1 VS 3000 mg/dry/TS/day 70.33153.51 ml 15006000 mg/VS 204 mL g1 VS N/R 130 mL g1 VS 2000 mg/l/VS 0.35 L g1 COD 1.3 0.45.8 0.9 287 mL g1 VS 2000 mg/TS/L 240 mL g1 VS 2000 mg/VS/L 170 mL g1 COD 1000 mg/COD/L 290 mL g1 VS 18,000 mg/VS/L 354 mL g1 VS 18,000 mg/VS/L 16.315.8 ft3 7.89.2 ft3/lb (VS) 143 mL g1 VS 4000 mg/VS/L 0.79 g/L 72,000 mg/L/TS 0.350.80 m3 20100 kg/m3 (VS) 320 mL g1 VS 910 mg/VS/L 330 mL g1 VS 22,500 mg/VS/L 0.40 m3 kg N/R 0.250.31 L g1 VS 2000 mg/VS 
497 mL g1 TS 2.16 g/L/TS 344 mL g1 TS N/R e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
production rate was attributed to the effects of salinity. The highestmethane production recordedwas by De Schamphelaire and Verstraete who recorded a gas production of 600 mL g1 VS for a mixed unde-termined freshwater microalgae consortium. The data shown in Table 1also highlights the difference in units and terminology used to reportgas production from microalgae. Units range from gas production pergrams of chemical oxygen demand (COD) destroyed, gas producedper gram of volatile solids loaded and gas produced per gram of totalsolids loaded. The standardisation of terminology and standard unitsto report biogas productivities are essential for comparing microalgaeand other digestible substrates.
Themethods used to determine volatile solids are also used to deter-mine the ash free dry weight (AFDW) of microalgae [36,37]. Ash freedry weight is used extensively by phycologists to report quantities ofmicroalgae biomass. When reporting microalgae biomass, the ash freedry weight or the volatile solids (digestible component) of themicroalgae biomass is a percentage of the total solids and varies be-tween species. The data in Table 2 includes the AFDW or VS of somecommon microalgae species. The variation in AFDW and VS can varyby up to 50% between species and can signicantly affect predictingthe theoretical biogas production potential for the anaerobic digestionof microalgae.
Sanchez-Hernandez and Trvieso  paper no concentrating step wasreported and the chlorophyll a ranged from 2.87 mg/L to 9.62 mg/L.This higher chlorophyll a content would indicate that the microalgaewere at a higher density, and the problem of low volatile solids mayhave not been evident in the experiment.
In the De Schamphelaire and Verstraete  experiment the authorscame to the conclusion that a concentrating step would be required foroptimal performance of the anaerobic digestion process. Results indicatethat the digester completely failed once during the experimental period.The authors observed that the required volatile solids loading rate com-prising of microalgae biomass was too dilute and contained excessivewater, leading to the washout of the anaerobic bacteria community.Bacterial washout is due to a low digestible content of the wastewateror digestible feedstock. Hence when the subsequent hydraulic retentiontime within the digester is shortened to less than the bacterial genera-tional time, the result is a decreased bacterial population [44,45].
McCarty  indicated that a settling tank could be utilised after thedigester to allow bacteria and solids to settle via gravity. These solidscould then be reintroduced to the digester for further processing. Thisstep is essential to reduce bacterial washout when the hydraulic reten-tion time (HRT) is lower than the solid retention time (SRT) of thesubstrate.
4 A.J. Ward et al. / Algal Research xxx (2014) xxxxxx4. Problems with anaerobic digestion of microalgae
4.1. Low concentration of digestible substrate
The majority of authors listed in Table 1 conclude that the concen-trating or harvesting of microalgae biomass presents a fundamentalchallenge to the nancial viability of an energy system usingmicroalgaebiomass as a substrate for anaerobic digestion or alternative biofuelproduction.
Gouleke et al.  identied the lowvolatile solids loading rate that isassociated with microalgae when used as a digestible substrate. The lowVS rate is due to the low concentration of microalgae biomass present inlarge volume of water. Signicant research has focussed on engineeringissues associated with the, harvesting, dewatering, and further concen-trating of the microalgae biomass energy. Engineering issues commonin microalgae production for biofuel are discussed by Benemann et al., Chen et al. , and Molina et al. . Regarding the data pre-sented in Table 1, all experiments except for the work published bySanchez-Hernandez and Trvieso  and De Schamphelaire andVerstraete  were performed using concentrated microalgae. In the
Table 2The volatile solids (VS) or ash free dry weights (AFDW) as a percentage of the total solids
Species Fresh or saltwater
Arthrospira maxima BrackishBlue green algae FreshChorella vulgaris FreshChorella vulgaris FreshChlorogloeopsis fritschii SaltDunaliella sp. SaltwaterIsochrysis galbana SaltwaterNannochloropsis oculata SaltwaterNannochloropsis salina FreshNannochloropsis sp. SaltwaterNitzschia closterium SaltwaterPhaeodactylum tricornutum FreshPorphyridium cruentum SaltwaterScenedesmus dimorphus FreshScenedesmus obliquus FreshScenedesmus sp. FreshSpirulina maxima SaltwaterSpirulina platensis SaltSpirulina platensis FreshWastewater consortium FreshWastewater consortium (lipid extracted) FreshPlease cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001Bacterialwashout can also be addressed by better anaerobic digesterdesign. Zamalloa  used a laboratory scale membrane reactor to an-aerobically digest Phaeodactylum tricornutum. The addition of themem-brane to the reactor gave a hydraulic retention time of 2.5 days, whilethe solids retention time was increased to between 10 and 20 daysdepending on the solids loading rate. The decoupling of the hydraulicretention times and the solids retention time can also be achieved byutilising upow anaerobic sludge blanket (USAB) reactors, anaerobicmembrane reactors (AnMBR), anaerobic lters (AF) and anaerobicuidised bed reactors (AFBR)  and by fermentation cells  or byin-pond digesters [27,28].
The paper by Collet et al.  reports a novel approach to concentrat-ing microalgae. The authors rst use a gravity settling-step to separatethe microalgae before transferring it into a centrifuge for dewateringand concentrating to a higher percent biomass solid. The results indicat-ed that by settling the culture for 1 h, 65% of the microalgae biomasswas separated into slurry with a concentration 20 times higher thanin the original culture stream. The authors then used centrifugationand reported a further concentration factor of ve times. However thisinitial settling step was more effective with non-motile microalgaespecies.
reported for different microalgae species.
VS and AFDW as % of TS Reference
8093% 94% 93% 90% 92% 82% 86% 45% 77% 93% 78% 82% 91% 88% 72% 60% 86% 92% 93% 88% 86% e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
5A.J. Ward et al. / Algal Research xxx (2014) xxxxxxThe publications by Golueke et al. , Benamann et al.  andHarun et al.  investigated the use of chemical coagulation, occula-tion and centrifugation as a means of harvesting, concentrating anddewatering microalgae. All three papers discuss the high energy costsassociated with the use of centrifugation and occulation harvestingtechniques. Golueke and Oswald  identied that digester perfor-mance is unaffected by the centrifugation or by alum addition as a oc-culant. Their work has shown that concentrations in sludge up to 4%aluminium have no effect on digester stability or gas production.Many new commercially formulated coagulants exist and are com-prised of cationic and anionic poly-electrolytes, synthetic polyacryl-amide polymers and starch-based polymer occulants . Mostof these occulants are currently utilised in the wastewater treatmentindustry, and their use has shownvery fewdetrimental effects to digest-er stability or gas production [50,51].
The authors Kalyuzhnyi et al.  and Callander  have reportedimproved anaerobic digester performancewhen commercially availablechemical coagulants have been utilised. The increased performance isdue to better solid retention times of particulate matter, allowingmore complete digestion of solids and resulting in higher conversionsto biogas. Barford et al.  also noted that the use of the chemical oc-culants resulted in an increased biomass concentration in the digestercompared to the control that did not utilise a occulant. The authornoted that this higher concentration of particulate matter enabled con-siderably higher solids loadings per unit volume to be applied to thedigester. However the authors also noted that the higher concentrationof biomass could induce ammonia inhibition due to the much higherloading rates that could be applied to a digester with a occulated bio-mass. Zhang et al.  reported that a high pH by chemical adjustmentassociated with struvite formation had no negative effect to the perfor-mance of anaerobic digestion.
With the high cost associated with these harvesting and dewateringstepsmanynew cost efcient laboratory and pilot scale technologies areunder development. Many of these technologies are still to be proven infull commercial scale settings, and their impact to anaerobic digestion isyet to be established.
4.2. Cell wall degradability and pre-treatment of microalgae biomass
Golueke et al.  demonstrated the ability of microalgae to passthrough an anaerobic digester intact and remain undigested. Theauthors noted that microalgal cells are known to be able to effectivelyresist bacterial attack and found intact microalgae cells in digestateleaving a digester after a 30-day hydraulic retention time. Sanchez-Hernandez and Trvieso-Cordoba  observed that when C. vulgariswas added to a digester the chlorophyll a concentrations increasedwithin the digester for the rst two weeks of the experimental periodbut was still detectable 64 days after the start of the experiment. Zhouet al.  also found intact cells in digestate from a digester after45 days. The longest duration reported for intact microalgae cells sur-viving within an anaerobic digester was reported by Mussgnung et al.. They identied viable Scenedesmus cells after 6 months that hadswitched to mixotrophic growth.
Work byMussgnung et al.  highlighted the role of the cell wall inthe digestion process. Their results indicated that the higher gas produc-tion reported was due to the microalgae species that had either no cellwall or a cell wall made from protein. Gas production was observed todecrease for microalgal species that had a carbohydrate-based cell wallcontaining hemicellulose. The lowest gas production came from the spe-cies S. obliquus that has a particular rigid cell wall containing sporopol-lenin like biopolymers. Little or no cell wall degradation was detectedin S. obliquus and very little gas was produced by the substrate. The au-thors concluded that the degradation of the cell wall was strongly corre-lated to the amount of gas produced during anaerobic digestion [10,57].The results reported byMussgnug et al.  also correlate to ndings by
Ras et al.  who noted changes in cell wall chemistry and its inuence
Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001on substrate degradability. Their results indicate the need for a pre-treatment step to disrupt the cell wall and increase bacterial hydrolysisbefore addition to the anaerobic digester [10,23,25,47,5760]. Cell lysisis also essential for solvent extraction of the lipid fraction in microalgaebiomass , allowing solvents to react with internal cell lipids. Hencemicroalgal cell wall disruption processes are essential for both lipid-based biofuel applications and for optimal microalgae anaerobic diges-tion or co-digestion processes.
Golueke and Oswald  investigated a thermal pre-treatment stepof microalgal biomass wherein the temperature was raised above thethermal limit of the microalgal species, resulting in cell disruption.Chen andOswald  undertook experiments that investigated thermalpre-treatment combined with chemical pre-treatment using sodiumhydroxide and variable exposure times. Their results demonstratedthat all pre-treatments tested produced better results than untreatedcontrol comparisons. It was demonstrated that the most efcient pre-treatment for microalgal biomass required heating to 100 C for 8 hwithout an increase in pH using the addition of sodium hydroxide.This treatment increased gas productivity by 33% as compared to un-treated microalgae biomass. This study also indicated that up to 60% ofthe untreated microalgae biomass added to the anaerobic digesterwill remain undigested due to the cell wall remaining intact throughoutthe digestion process . More recent studies by Gonzalez-Fernandezet al.  reported that thermal pre-treatment of Scenedesmus sp. in-creased methane potential. At 70 C, a 9% increase in methane produc-tion was reported, which increased to 57% at 90 C when compared tountreated microalgae biomass. Further work by Gonzalez-Fernandezet al.  investigated the effect of the organic loading rates and thethermal pre-treatment of biomass at 90 C for 1 h. Results indicatedthat a 2.9 and 3.4 fold increase in methane production for organic load-ing rates of 1 and 2.5 kg COD m3 day respectively. Research reportedby Alzate et al.  showed an increase of 46% to 62% in methaneproductivity utilising thermal hydrolysis. However results from DeSchamphelaire and Verstraete  reported no benet when pre-treating a mixture of Chlorella, Pseudokirchneriella and Chlamydomonasmicroalgae species at 80 C for 2.5 h.
Samson and Leduy  investigated thermo-chemical (heat andsodium hydroxide addition), mechanical and ultrasonic disintegrationpre-treatment methods. The authors reported that at a temperature of50 C there was a 20% increase in substrate solubilisation and at150 C there was a 43% increase in substrate solubilisation. Their exper-iments indicated that the ultrasonic treatment gave similar results asthe 150 C heat treatment. The time taken for the ultrasonic treatmentwas relatively short and only took 10 min compared to 1 h for the ther-mal pre-treatment. Paris and Oswald  and Samson and Leduy both indicated that the simple addition of sodium hydroxide was inef-cient as a pre-treatment step for the anaerobic digestion of microalgaebiomass or biosolids.
Gonzalez-Fernandez et al.  investigated sonic disruption pre-treatment of microalgae biomass. They utilised a frequency of 20 Hzbut at varying power levels. All sonicated biomass exhibited highermethane production during the rst days of digestion compared to un-treated biomass. Overall the highest microalgae biodegradability of 44%was recorded for the longest sonication treatment as compared to 23%for un-sonicated biomass.
Samson and Leduy  reported a 26% increase in the solubilisationof microalgal substrates by freezing the biomass. This was due to thedisruption of the microalgae cell wall by ice crystals. Keymer et al. adapted high pressure thermal hydrolysis (HPTH), a commerciallyavailable technology used for the disruption of waste activated sludgebiosolids for the purpose of pre-treatingmicroalgae biomass. HPTH pro-cesses heat substrate to approximately 160 C at a pressure of approxi-mately 6 bars. After these conditions have been maintained for2030 min the contents are then reduced in pressure via a ash drumwhere the pressure change causes the cells to rupture and release the
cell contents. Keymer et al.  reported that the process substantially
e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
57328 mL/day of biogas, whichwas about a 50% reduction in gas pro-
6 A.J. Ward et al. / Algal Research xxx (2014) xxxxxxincreased methane potential for lipid extracted and non-lipid extractedalgae. The authors also reported an extraction method using a soxhletapparatus with hexane to extract the lipid that increased the bio-methane potential of the microalgae biomass. When both lipid extrac-tion and HPTL were combined an increase in the digestibility of thelipid extracted and HPTL microalgae biomass of 110% was recordedcompared to untreated microalgae biomass. However this process isenergy intensive but energy balances demonstrate that HPTL coupledwith anaerobic digestion can be energy positive due to the increasedmethane potential from the substrate .
The various mechanical, physical, thermal and chemical methodsused to improve microalgae methane potential can have a high energyrequirement. Several authors have found that the energy consumptionfor the pre-treatment of microalgae biomass is equal to or higher thanthe energy gained from the microalgal cell [42,59,6669]. Due to thishigh energy demand alternative methods including enzymatic and bac-terial methods have also been investigated. Lu et al.  cited results bySander andMurthy  wherein they reported the cell walls of amixedmicroalgae culture to be susceptible to degradation by lipase and cellu-lase. Results reported by Ehimen et al.  showed an increase inmeth-ane production by treating Rhizoclonium biomass with the addition ofan enzymatic mixture. The greatest increase in gas production resultedfrom the addition of the single enzyme cellulase. Bacterial cell disrup-tion has also been shown to increase methane production . The au-thors Lu et al.  demonstrated an increase of 1724% in biogasproduction by adding the bacterium Clostridium thermocellum toC. vulgaris biomass.
4.3. The carbon/nitrogen ratio associated with microalgae biomass
Vergara-Fernandez et al. , Sialve et al.  and Yen and Brune identied further difculties with the anaerobic digestion ofmicroalgae biomass, due to the low carbon to nitrogen ratio present inmicroalgal species. Data reported in Table 1 shows that the carbon/ni-trogen (C/N) ratio varies from 4.16 to 7.82 for microalgal species thathave been investigated for anaerobic digestion. When the C/N ratio isbelow 20 there is an imbalance between carbon and nitrogen require-ments for the anaerobic bacterial community or consortia . This im-balance leads to nitrogen release in the form of ammonia duringdigestion, which can become inhibitory to methanogenic bacteria andresult in volatile fatty acids accumulating within a digester .Ammonia-nitrogen and volatile fatty acids (VFA) are important inter-mediates in anaerobic digestion processes but can also be potential in-hibitors when allowed to accumulate .
To overcome problematically low C/N ratios, several researchershave investigated co-digestion, were microalgae has been co-digestedwith other waste streams or biomass to increase the C/N ratio. Thesestudies include Gonzalez-Fernandez et al.  and Shouquan et al. who investigated the addition of microalgae to pig manure priorto digestion. Saxena et al.  recorded increased methane productionfor the anaerobic co-digestion of green lamentous microalgae andwater hyacinth supplemented with cow manure. Ramamoorthyand Sulochana  also investigated the addition of Zygogonium sp.with various quantities of cow manure. Shuchuan et al.  reported asignicant increase in methane potential when blue green algae wasco-digested with corn stalks. Samson and Leduy  undertook a co-digestion experiment wherein they blended sewage sludge with Spiru-lina maxima and observed a 2-fold increase in gas production when amixture of 50% by weight of sewage sludge to microalgae ratio wasused. Yaun et al.  reported increased methane potential in the co-digestion ofmunicipalwastewater solids with Chlorella sp. and Spirulinaplatensismicroalgae species respectively. Park and Li  investigatedco-digestion using Nannochloropsis salina and lipid-rich fats, oil andgrease. They observed higher methane production and were ableto use an increased organic loading rate due to a more balanced C/N
ratio with less inhibition and digester imbalance. An increase in gas
Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001duction compared to the more favourable C/N ratio treatment. Yen andBrune  concluded that the best C/N ratio for anaerobic digestion isin-between 20:1 and 25:1. This conclusion is similar to the C/N ratiodiscussed by the authors Parkin and Owen  wherein they indicatedan optimum C/N ratio range of between 20:1 and 30:1.
Yen and Brune  indicated that as the C/N ratio increased theamount of total ammonia-nitrogen decreased as the C/N ratio becamemore favourable thus reducing ammonia-nitrogen inhibition effect.Ehimen et al.  suggested that a C/N ratio of 15 or below can resultin a build up of free ammonia-nitrogen,which can be detrimental to an-aerobic digestion processes. The high C/N ratio treatment of 36.4/1 usedin the experiment was on the upper extreme for anaerobic digestion ashigh volatile fatty acid (VFA) concentrations can also become inhibitoryto anaerobic digestion. At a high C/N ratio, the amount of totalammonia-nitrogen can be too low for the cellular needs of the anaerobicmicroorganisms. It has been shown that aminimumconcentration of 50to 200mg/L of nitrogen as ammonia is essential for the requirements ofthe bacterial community associated with anaerobic digestion [44,84].The co-digestion of two substratesmay improve C/N ratio andVFA/alka-linity ratios and attenuate unfavourable ratios in a single substrate.
One problem thatmust be consideredwith the co-digestion of a sec-ondwaste streamor biomass is the seasonal availability of the feedstockand location of production. This problem was highlighted previouslywhere seasonal growth of macroalgae limited anaerobic digestion toonly six months of the year [11,17].
4.4. Lipids and microalgae
Lipids are an attractive substrate for anaerobic digestion and have ahigher theoretical methane potential compared to proteins and carbo-hydrates . However due to their low alkalinity and buffering capac-ity, lipids can cause inhibition due to their intermediate products suchas long chain fatty acids (LCFAs) and VFAs . It has been suggestedthat the conversion of microalgal biomass to methane rich biogas isenergetically more favourable than lipids removal frommicroalgae bio-mass with the total lipid content is lower than 40% . However, theremoval of lipids frommicroalgae biomass for liquid biofuel productionprior to anaerobic digestion of the residual microalgae biomass can bebenecial to anaerobic digestion processes, as high lipid concentrationscan be inhibitory [59,80,86].
Crine et al.  reported that there was no inhibition for lipid con-centrations of 5, 10 and 18% respectively. However inhibition was ob-served for lipid concentrations of 31, 40 and 47%, where inhibitionincreased due to higher lipid fractions. It is anticipated that the lipidproduction was also reported by Salerno et al.  by co-digesting do-mestic municipal wastewater grown microalgae and soybean oil.
Glycerol is a carbon-rich by-product of the trans-estericationconversion of lipids to biodiesel, and it can be used as a carbon sourceto maximise gas production in anaerobic co-digestion . Ehimenet al.  investigated using glycerol produced from transesteriedmicroalgae lipid. A slight increase in gas production rate was noted inthis study; however a low application rate of glycerol was utilised. Anincrease in gas production was also observed by Salerno et al. when co-digesting glycerol and domestic municipal wastewater de-rived microalgae biomass.
Yen and Brune  considered the addition of paper waste to im-prove the C/N ratio of the microalgae combination comprised ofScenedesmus sp. and Chlorella sp. Yen and Brune  illustrated thatwith the addition of waste paper there was an increase in the C/N ratiofrom 6.7 to 36.4. Results from this experiment showed that the bestco-digestion ratio was 50% paper and 50% microalgae. The nal C/Nratio of this combination was 18.0 with 1170 75 mL/day biogas pro-duced. In comparison themicroalgae-only anaerobic digestion producedconcentration for economically viable lipid-based biofuel microalgae
e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
species generally exceeds 30%, which could have negative conse-quences for anaerobic digestion if the lipids are not extracted.
Lipid extraction methods used on microalgal biomass can affect thedigestibility of residual microalgal biomass. Ehimen et al.  andThiel  reported a signicant decrease in gas production due to resid-ual chloroform from the Bligh and Dyer extraction process even thoughit had been heat treated to remove residual entrained solvents after theextraction process . Butanol, hexane and methanol have beenshown to have no detrimental effects on anaerobic digestion when re-sidual solvents are removed by heating .
5. Theoretical methane production
When the C, H, O and N composition of a wastewater or substrate is
release of ammonia-nitrogen during anaerobic digestion . Theequilibrium established between un-ionised ammonia- (NH3-N) andammonium- (NH4-N) nitrogen can be affected by a change in pH ortemperature within the anaerobic digester. An increase in temperatureor pH can be very detrimental to the bacterial community as the equilib-rium shifts to the more toxic un-ionised form of ammonia-nitrogenNH3-N [44,59,92].
McCarty  indicated that ammonia gas within the digester is in-hibitory at a much lower concentration than the aqueous ionised formof ammoniumnitrogen. Ammonia toxicity has been shown to affectmethanogenic bacteria in twoways: (1) the ammonium ionmay inhibitthe methane synthesising enzyme directly, and (2) the hydrophobicammonia-nitrogenmoleculemay diffuse passively into the cell, causingproton imbalance and/or potassium deciency [59,94]. Ammonia-
7A.J. Ward et al. / Algal Research xxx (2014) xxxxxxknown, the stoichiometric relationship reported by Buswell and Boruff can be used to estimate the theoretical gas composition on a per-centage molar basis.
CaHbOcNd 4ab2c 3d
4ab 2c 3d8
where a, b, c and d equal the carbon content, hydrogen content, oxygencontent and nitrogen molar composition respectively [59,89,90].
Methane yield L=g VS destroyed 4a b2d3d12a b 16d 14d
where Vm is the molar volume of methane or 22.14 L at 0 C and 1 atm. Eq. 2 is used to calculate the volume of methane gas depending onthe amount of volatile solids (VS) available in the substrate beingdigested.
The data shown in Table 3 illustrates the theoretical methane poten-tial for several microalgae species utilising Eq. 2 and values from litera-ture. However Eq. 2 overestimates the gas production as it assumes100% conversion of the volatile solids to biogas and also does not consid-er the needs for bacterial cell maintenance and anabolism [59,90].When the theoretical methane potential was calculated for lipid ex-tracted and non lipid extracted wastewater microalgae consortium re-ported in Chinnasamy et al. , a 13% decrease in the theoreticalmethane potential was reported highlighting the residual gas potentialof lipid-extracted microalgae biomass.
6. Inhibition of anaerobic digestion
6.1. Ammonia-nitrogen toxicity
Ammonia-nitrogen is produced from thebiological breakdownof ni-trogenousmatter, mostly in the form of proteins and urea . The highnitrogen and protein levels found in microalgae can lead to signicant
Table 3Biochemical analysis of microalgae species and their theoretical methane potentials calcul
Species Carbon Hydrogen N
Chlorogloeopsis fritschii 54.4 2.1 6.9 0.5Spirulina platensis 55.7 0.4 6.8 0.1Chlorella vulgaris 52.6 0.8 7.1 0.1Scenedesmus dimorphus 53.4 0.6 7.8 0.2Wastewater consortium 49.4 0.1 6.7 0.1Wastewater consortium (lipid extracted) 45.9 1.9 6.2 0.1Nannochlorospsis sp. (lipid extracted) 49.7 7.1Nannochlorospsis sp. 47.6 6.6Nannochlorospsis sp. (low lipid) 52.0 7.5Nannochlorospsis sp. (high lipid) 51.5 7.3Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001nitrogen is toxic at high levels and has a moderately inhibitive effectfrom 15003000 mg/L. Above 3000 mg/L there is a strong inhibitiveeffect associated with ammoniumnitrogen , which can lead to adrop in gas production. Inhibition of the methanogenic or acidogenicgroups of anaerobic microbes was not quantied in this study.
There is a large amount of conicting information in the literaturerelating to the ammonia-nitrogen tolerance of anaerobic microbes.Research based on methane production and growth rate comparisonsindicate that inhibitory effects are greater for the acidogenic bacteriacompared to the methanogenic bacteria [84,95100]. It has been ob-served that acetate consuming methanogens have relatively high resis-tance to high ammonia-nitrogen concentrations [84,94,100102].
This difference in opinion demonstrates the distinctive responses thatcan be associated with bacterial consortiums that are involved withanaerobic digestion. Among the methanogenic strains commonly foundin digesters (for example: Methanospirillum hungatei, Methanosarcinabarkeri,Methanobacterium thermoautotrophicum, and Methanobacteriumformicicum) the speciesM. hungatei was found to be the most sensitivemicrobe to ammonia-nitrogen, with inhibition observed at 4200 mg/Lcompared to the other three strains where inhibition did not occur untilammonia-nitrogen levels were above 10,000 mg/L . The inhibitionof M. hungatei at 4200 mg/L is the only methanogenic strain out of theve methanogenic strains tested that corresponds to the ndings ofParkin and Owen .
The utilisation of volatile fatty acids by methanogens must balancethe production of volatile fatty acids by hydrolytic and acetogenic bacte-ria in order tomaintain digestion stability. Efcient digester performanceis therefore dependent upon maintaining the ammonia-nitrogen con-centration below the inhibitory limits for all of the associated digestionbacteria .
A solution to the problem of digester stability and balancing the bac-terial populations and end products is to separate the bacterial commu-nities. This can be done by utilising a two-stage anaerobic digestionprocess . Themetabolic pathways of the two-stage anaerobic diges-tion processes are the same as those of single-stage anaerobic digestion.However the stages are physically separated with the hydrolytic andacetogenic bacteria in the rst stage and the methanogenic bacteria in
from the mean carbon, nitrogen, hydrogen and oxygen values.
en Oxygen Calculated methane potential (ml/g/VS) Reference
3 0.3 31.4 309 2 0.1 26.4 319 2 0.2 32.2 283 9 0.1 31.0 260 3 0.2 21.6 347 3 0.9 23.6 303 8 26.7 340 5 21.7 383 8 22.4 414 5 22.4 414 e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
microalgae loaded using a two-stage digestion process to obtain theseresults. However this work did not quantify the salinity of the concen-
8 A.J. Ward et al. / Algal Research xxx (2014) xxxxxxtrated paste that was used to feed the digester, and the actual salinityof the substrate was less than that of seawater salinities.
Asinari Di San Marzano et al.  showed that a gas production of310ml and 440mL g1 VS for dry anaerobically digested Tetraselmis sp.of saline microalgae. Both methane production rates are for Tetraselmissp. microalgae biomass that has a salinity of b1 g/L. However they alsorecorded 450 mL g1 VS added with a substrate salinity of 35 g/L. Theauthors did not indicate whether this is for dry or wet Tetraselmis sp.,and they did not indicate the source of inoculum used for this experi-ment. Buxy et al.  reported a methane potential of 204 mL g1
VS for the marine microalgae Nannochloropsis oculata. This microalgawas grown under seawater salinity conditions, harvested, concentratedto a paste and then used for the bio-methane potential experiments.Due to the use of concentrated paste and its addition to a freshwateranaerobic digester, the nal salinities of the digestion vessel are notreported.
High salinity levels have been shown to be inhibitory as it can causebacterial cells to dehydrate due to increased osmotic pressure .Salinity is made up of multiple elements and can vary depending onthe source of water and its associated environment . The lightmetal ions including sodium, magnesium, calcium and aluminium canthe second phase . Several authors have indicated that by usingtwo-stage digestion there is increased degradation of organic matter,improved biogas production and better control over the conditionsinside the digester limiting inhibition of the microbial populations[72,104106].
Yang et al.  demonstrated that by utilising a two-stagedigestion process an increased methane yield of 22% was achievedwhen anaerobically digesting Scenedesmus sp. The authors also recorded46 ml g1VS of hydrogen production in the rst stage of the two-stagedigestion process. The hydrogen production was in addition to themethane production recorded for the second stage. A soluble CODreduction of 75% was recorded for the single stage digestion, whereas asoluble COD reduction of 81.8% was recorded for the two stage process.
Recent work by Inglesby and Fisher  has shown that the integra-tion of microbial fuel cells can be benecial in decreasing ammonia-nitrogen inhibition during anaerobic digestion. Improved performanceis achieved by allowing the ammonium ion to migrate across the cationexchange membrane from the anode to the cathode. The use of themicrobial fuel cells decreases the chance of free ammonia-nitrogen inhi-bition ofmethanogenic bacteria improving the stability of the anaerobicdigestion process .
6.2. Saline microalgae and the effect of salinity
Some species ofmicroalgae that have been identied for their poten-tial as feedstock for liquid biofuels are grown in a saline environment.The use of saltwater for their production offers a sustainable alternativedue to the ability to use non-arable land and seawater, hence reducingpressure on current agricultural land and scarce freshwater resources.Several scientic publications cited in Table 1 have dealt with salinespecies of microalgae. The marine species Macrocystis pyrifera andTetraselmis sp. have been used as a substrate for anaerobic digestion.The cyanobacteria Spirulina sp. has also been identied for having thepotential to be grown in saline waters .
Alkaline earth metal salts are needed in very low concentrations forcellular metabolism in bacteria, and higher concentrations can be ex-tremely toxic to methanogenic bacteria . Salinity and more speci-cally sodium divalent cations do pose a problem to bacteria associatedwith anaerobic digestion [44,93]. Vergara-Fernandez et al.  demon-strated that the digestion of marinemicroalgae is possible. They record-ed a total biogas production of between 95 and 260 mL g1 TSall be toxic at high levels [84,93].
Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001The sodium ion is themost inhibitory to anaerobic digestion of thesemetal ions, and it makes up a larger percentage of the light metal ionsfound in seawater . However inhibition due to sodium varies de-pending on the source of inoculum and overall elemental compositionof the saline water and substrate being digested . Rinzema demonstrated that sodium concentrations of 5, 10 and 14 g/L caused10, 50 and 100% inhibition in acetoclastic methanogen bacteria respec-tively. These measured sodium inhibition rates were also observed byParkin and Owen  who reported a moderate inhibitory effect onanaerobic digestion at sodium concentrations ranging from 3.5 to5.5 g/L. Above 8.0 g/L of sodium can be extremely inhibitive.
However, Parkin and Owen  noted that amelioration of sodiumions and potassium ions was possible. When this amelioration occurredit could reduce the toxicity caused by light metal ions during anaerobicdigestion. In addition, the presence of sodium ions was found to be an-tagonistic to ammonia-nitrogen inhibition. Experiments by Kugelman demonstrated that at an ammonia-nitrogen concentration of0.15 mol/L, the methane production from acetic acid was reduced by20%. The addition of 0.0020.05 mol of sodium (Na+) produced 5%more methane compared to that of the control. This research indicateda further increase of 10% in methane production was achieved by usinga combination of sodium and potassium or sodium andmagnesium cat-ions .
Zhang et al.  identied that sodium induced build up of propio-nate to be problematic in their digester system, which became inhibito-ry and caused digester pH imbalance and digester failure. Zhang et al. offered a solution to overcome the inhibition from sodium insyntrophic acetogenic bacteria. To help address high sodium inhibition,Zhang et al.  utilised an electrical current delivered to the digestervia an iron anode and graphite cathode. They found by adding an elec-trical current to the digester enough free energy was produced to con-vert propionate acid to acetic acid allowing methanogens to furthertransform the acetic acid to methane .
6.3. Sulfur and its role in anaerobic digestion
Freshwater microalgal biomass contains low levels of sulfuratedamino acids and their digestion releases lower amounts of hydrogensulde than other types of substrates . However oxidised sulfurcompounds can be present in saline waters and saline substrates.These sulfur compounds can act as electron acceptors for sulfate-reducing bacteria that convert organic compounds in an anaerobic reac-tor and produce hydrogen sulde gas. Hydrogen sulde when presentin gas is corrosive and can cause damage to machinery, such as gas-engine power generators, and piping . Except for sulde, sulfurcompounds are not harmful to anaerobic bacteria unless at highconcentrations.
Sulde is needed for cellular metabolism in low concentrations bybacteria, but concentrations higher than 200 mg/L become extremelytoxic to methanogenic bacteria[44,114]. Also like un-ionised ammonia-nitrogen, un-ionised sulde is much more toxic than ionised sulde. Thespeciation between the two compounds is also dependent on tempera-ture and pH 
Sulfate reducing bacteria compete with methanogenic bacteria foracetate and hydrogen. The sulfate reducing bacteria have a higher afn-ity for acetate than methanogens, outcompeting them under low ace-tate concentrations . This competitive inhibition results in theshunting of electrons from methane generation to sulfate reduction.Sulfate reducers and methanogens are very competitive at COD/SO4 ra-tios of 1.7 to 2.7. An increase of this ratio is favourable to methanogens,whereas a decrease is favourable to sulfate reducers [90,114].
The conclusion on the optimum COD/SO4 ratio is supported by thepaper by Aspe et al. wherein they foundmethane fermentation in-hibition at a COD/SO4 ratio lower than 0.5. Aspe et al.  highlightedthe difference between inoculums used for seeding anaerobic digesters.
Their research focused on two inoculums: one was sourced from
e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
9A.J. Ward et al. / Algal Research xxx (2014) xxxxxxpiggery efuent and the other was sourced from a marine sediment in-oculum. The authors indicated that the sulfate reducing bacteria did notgrow as well in the marine sediment sourced inoculum as compared tothe piggery efuent sourced inoculum.
7. Bacterial consortium and its role in anaerobic digestion
Aspe et al.  highlighted the different results that can be obtain-ed from the source of the bacterial inoculum. Little work has been doneon bacterial communities within themicroalgal anaerobic digester, andthere is a lack of information in the scientic literature on this topic.
The authors Zhang et al.  and Patil et al.  used polymerasechain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) toprole the microbial communities associated with anaerobic digestion.This analysis revealed the presence of unknown and as yet unculturedmicroorganisms associated with the digestion process. Unknown or-ganisms and differences within anaerobic communities are problematicwhen comparing the variety of results from similar substrates for anaer-obic digestion. The inoculum source and the associated bacterial speciescan change over geographical and environmental locations.
The ability of bacteria to utilise environmental plasticity to changeand adapt to different substrates and environmental conditions overtime is reported by Buxy et al. , who reported an adaptation ofbacteria to saline conditions when undertaking bio-methane potentialexperiments shown by an increase in gas potential and a decrease inthe initial lag phase of the digestion process. Further investigation ofthe microbial community's adaptation and environmental plasticitycould potentially offer improvements in the bacterial metabolism andthe anaerobic digestion process under adverse conditions.
8. Anaerobic digestion and nutrient recycling
Nutrients are a large and expensive input into the mass productionof microalgal biomass. Large amounts of nitrogen and phosphorousare needed for algae biomass production.With the proposed expansionof the commercial algal biomass production industry, the competitionwith current agricultural industries for organic fertilisers is expectedto increase , which could increase fertiliser prices. Historicallyfertiliser prices are closely related to the cost of fossil fuel, and as fossilfuels becomes more expensive fertiliser prices will also increase[5,117]. This increase in oil prices combined with greater fertiliser andenergy demand from the agricultural sector could result in increasedoil prices making inorganic fertiliser un-competitive for algal biofuelproduction . Due to these compounding factors, nutrient recoveryfrom processed biomass via anaerobic digestion is highly desirable forthe sustainable growth of the algal biomass.
Lyovo et al.  indicated that 45 kg of nitrogen is needed for oneton of algal biomass based on a composition of CO0.48H1.83N0.11P0.01. When analysing this stoichiometric relationship, an 11:1 ratioof nitrogen to phosphorus can be determined. This nitrogen to phos-phorus ratio indicates that approximately 4 kg of phosphorous is re-quired for every one tonne of algal biomass grown. This ratio furtherhighlights the role that anaerobic digestion provides in the recyclingof nutrient that is vital to the sustainability and economic feasibility ofcommercial-scale algal biofuel industries [1,59,64,80].
Phosphorus as a nutrient has had very little research attentionwhencompared to other nutrients such as carbon and nitrogen . Vaccari stated, the on-going supply of phosphorus has become one themost signicant sustainability issues facing our future .
Several authors have highlighted that there are nite reserves ofrock phosphorus available and human civilisation is heading towardsa similar scenario as peak oil except with dwindling phosphorus re-serves. However the exact quantity of reserves and timing of the short-age is still highly disputed [117,119,120]. This future decline in availablephosphorus reserves must be addressed for the sustainable growth of
algae for commodity products such as biofuel .
Please cite this article as: A.J. Ward, et al., Anaerobic digestion of algaj.algal.2014.02.001Anaerobic digestion of algal biomass produces a nutrient-richdigestate containing both nitrogen and phosphorus nutrients. Digestatenutrient values of 2940 mg/L ammonia-nitrogen, 390 mg/L of totalphosphorous, and 320 mg/L of potassium have been reported by Colletet al. . However, these values are in the moderate to high inhibitionranges recorded for ammonia-nitrogen by Parkin and Owen .In contrast, studies conducted by Zamalloa  indicated a clear liquiddigestate was produced with a total ammonia-nitrogen (TAN) concen-tration of 546 48 mg/L and a phosphate concentration of 141 41 mg/L from anaerobically digested P. tricornutum. These results dem-onstrate the high strength nutrient-rich digestate that can be producedfrom the anaerobic digestion of microalgae biomass. Results from DeSchamphelaire and Verstraete  also indicate that the high strength,nutrient-rich digestate nitrogen to phosphorous ratio from several di-gestion experiments was in the range of 10 to 17:1 that is ideal for thecultivation of algal species.
The gross chemical composition of microalgae can be highly depen-dent upon environmental conditions such as light intensity, tempera-ture and nutrient availability. Generally microalgae contain varyingproportions of proteins, lipids, carbohydrates, nucleic acids, pigmentsand vitamins . The mineral composition of microalgae also meetsthe nutrient and mineral requirements for the microbial micro-orathat are associated with the anaerobic digestion process .
The use of digestate fromdigestedmicroalgae biomass is highlightedin the research by Asinari Di San Marzano et al. , Benemann et al., De Schamphelaire and Verstraete , Gonzalez-Fernandez but rst by Golueke and Oswald . Golueke and Oswald  andRas et al.  both setup a closed looped system where microalgae wasgrown and then harvested and immediately digested to produce biogas.The digestate from the anaerobic digester was then fed back into thehigh rate pond or photo-bioreactor and used as a nutrient source forfurther microalgae growth.
A further synergistic benet of integrating anaerobic digestion witha algal biofuel program is the ability to utilise themicroalgae cultures forpurifying the methane content of the biogas [12,59,85]. The concentra-tion of carbon dioxide derived biogas from anaerobically digestedmicroalgae is in the range of 30 to 50% . From an energy recoveryperspective the biogas CH4/CO2 ratio needs to be above 1  indicatingthat a gas purication step is required for microalgae derived biogas.Due to the low solubility of methane and high solubility of carbon diox-ide, uptake of carbon dioxide is high leaving high concentrations ofmethane after the purication step . Also during this process otherimpurities such as hydrogen sulde are removed from the biogas .Biogas CO2 bio-xation by microalgae is discussed by Green et al. .
Research undertaken by Converti et al.  also highlighted an in-creased methane percentage in the biogas produced by utilising themicroalgae culture to strip carbon dioxide gas from the biogas as it isproduced. This is similar to the highmethane gas composition recordedwith the advanced integratedwastewater pond systemutilising in pondfermentation pits . Methane has been shown to be non-detrimentalto microalgae growth and the upgrading of biogas via high densitymicroalgae cultures would be benecial due to the supply of carbon di-oxide to microalgae cultures. The carbon dioxide that is stripped fromthe biogaswould be utilised as a nutrient source by themicroalgae .
Early and more recent research have provided greater understand-ing of the complexity of individual algal species as a substrate for anaer-obic digestion or co-digestion. This knowledge will be extremelybenecial to the anaerobic digestion of algae andwill allow themethaneproduction rates from individual algae species to be increased andoptimised. Each individual species of algae must be treated differentlyand processed specically to optimise biogas yields. Anaerobic digestionof algal biomass is a key unit process that integrates efciently and ben-
ecially into the production of algae-based biofuels and algae-based
e biomass: A review, Algal Res. (2014), http://dx.doi.org/10.1016/
10 A.J. Ward et al. / Algal Research xxx (2014) xxxxxxwastewater treatment. The integration of algae-based biofuel produc-tion with the anaerobic digestion of algae residuals is the most applica-ble scenario for the maximisation of methane-rich biogas.
Several technical issues including the low concentration of digestible(biodegradable) algal substrates and cell wall disruption can be over-come by the pre-treatment methods used to process algae for liquidor gaseous biofuels. The integration of anaerobic digestion into pro-posed algae-derived biodiesel operations has the benet of being ableto utilise glycerol, a by-product, in a co-digestion with microalgae im-proving the carbon to nitrogen ratio and thus increasing gas production.Gas production can also be utilised for electrical or thermal energy pro-duction, while algal cultures can also be utilised for biogas upgrading.
The resulting digestate can improve efciency as it has been shownto be an ideal nutrient source for the production of algal biomass. Theutilisation of this digestate for regrowth of additional algal biomasswill help to close the nutrient loop associated with large scale algal bio-mass production and to achieve more widespread environmentalsustainability.
With a greater understanding of algal species and their growth andbiological characteristics, the anaerobic digestion of macroalgae andmicroalgae, and their residues, can be optimised to play a promisingrole in the sustainable future of clean energy derived from algalbiomass.
This researchwas conducted as part of an Australian Research CouncilLinkage Project (project number LP100200616). The views expressedherein are those of the authors and are not necessarily those of theAustralian Research Council.
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Anaerobic digestion of algae biomass: A review1. Introduction1.1. Algae based biofuels1.2. Historical perspective of anaerobic digestion
2. Macroalgae and anaerobic digestion3. Microalgae and anaerobic digestion3.1. Historical and current perspectives
4. Problems with anaerobic digestion of microalgae4.1. Low concentration of digestible substrate4.2. Cell wall degradability and pre-treatment of microalgae biomass4.3. The carbon/nitrogen ratio associated with microalgae biomass4.4. Lipids and microalgae
5. Theoretical methane production6. Inhibition of anaerobic digestion6.1. Ammonia-nitrogen toxicity6.2. Saline microalgae and the effect of salinity6.3. Sulfur and its role in anaerobic digestion
7. Bacterial consortium and its role in anaerobic digestion8. Anaerobic digestion and nutrient recycling9. ConclusionsAcknowledgementsReferences